PMID(sorted descending)
[localization on the shigella flexneri f6s chromosome of a rfa gene implicated in the biosynthesis of bacterial cell wall lipopolysaccharide]. 197558625
a general method for the isolation of non-sense suppressor strains in gram-negative bacteria. 197558594
[sensitivity of dysentery bacteria to furazolidone and phthalazol according to the materials of medical epidemiological station of odessa-kishinev railroad]. 197658090
method for identifying salmonella and shigella directly from the primary isolation plate by coagglutination of protein a-containing staphylococci sensitized with specific antibody.a technique is described that allows presumptive identification of either salmonella or shigella organisms directly upon the original isolation plate, in this case, macconkey agar. this was accomplished by applying a drop of specifically sensitized protein a-containing staphylococcus aureus over a "suspected" colony or several colonies of organisms grown on macconkey agar. the plate is tilted to and fro to allow mixing of the particles with specific antigen that ir readily solubilized from the c ...197657969
ultrastructural localization of viral antigens using the unlabeled antibody-enzyme method.employing the unlabeled antibody enzyme method at the ultrastructural level, a comparison was made between preembedding staining and postembedding staining for the detection of viral antigens. the bacteriophage p1 absorbed to the surface of shigella dysenteriae was used as a model system. preembedding staining resulted in the specific deposition of peroxidase-antiperoxidase (pap) complexes as an electron-dense coating around the viral heads. disadvantages of the preembedding staining method incl ...197657192
role of a lipopolysaccharide gene for immunogenicity of the enterobacterial common is known that only certain strains of the family of enterobacteriaceae, notably rough (r) mutants with the type r1 or r4 core, evoked antibodies in high titers against the common enterobacterial antigen (ca) after immunization of rabbits with heated cell suspensions. the present investigation deals with genetic and immunochemical aspects of certain r1 and r4 mutants isolated from escherichia coli 08 and various shigella serotypes which, unexpectedly, do not induce ca antibody formation. immun ...197657114
antigenic determinants of murein lipoprotein and its exposure at the surface of enterobacteriaceae.murein lipoprotein from the outer membrane of escherichia coli could be fixed to erythrocytes without pretreatment of the erythrocytes. passive hemagglutination or immune hemolysis could thus be used as sensitive assays to determine antibodies against lipoprotein. in rabbit antisera prepared against whole cells of e. coli, salmonella, arizona, and shigella antibodies against lipoprotein were present. the respective titers were lowest in encapsulated smooth strains and highest in rough mutants. a ...197657056
the unfortunate role of precedent in bacteriology. i. the main antigens of salmonellae: the proteins.proteins from s. paratyphi b were found to be better immunogens, in rabbits, than the bacteria from which they were extracted. likewise, proteins from s. newport induced antibodies which reacted in agar gel, against proteins from many species of salmonella. the immunogenicity of the bacterial proteins, as well as their many biological activities are ignored because the classic techniques used lose these main antigens of enterobacteriaceae. the contrasting overemphasized role attributed to the li ...197656833
[obtaining purified preparations of individual shigella newcastle k- and o-antigens by using an ultracentrifugation method].a modified method of obtaining the purified k- and o-antigens of sh. newcastle with the use of ultracentrifugation is described. serologically individual k- and o-antigens were obtained and their chemical composition was studied. polysaccharide of the k-antigen consisted of the galacturonic acid, galactose, glucose, rhamnose xylose and hexosamine; it contained no heptoses. the o-antigen contained galactose, glucose, mannose, rhamnose, heptose and hexosamine; in difference to k-antigen was equal ...197555023
[study of the role of type-specific antigen in the process of shigella flexneri interaction with epithelial cell and macrophage cultures].epithelial cells of the hep-2 line infected in parallel by genetically connected strains of sh. flexneri, differing in the capacity to synthesize the type antigen were studied morphologically. the type antigen proved to show no significant influence on the penetration of dysentery bacilli into the cell. a study of the process of phagocytosis of the mentioned strains by a culture of peritoneal macrophages of guinea pig demonstrated that the presence of a type antigen communicated to the bacteria ...197555022
[characteristics of colicinogenotypes in shigella sonnei]. 197554507
[combined use of rivanol in the media with antibiotics in the isolation of shigella]. 197554496
[study of the ability of shigella sonnei and flexneri to inactivate antibiotics]. 197554495
[beta-galactosidase activity of shigella]. 197554494
[procedures for selecting the ingredients to increase nutrient medium electiveness]. 197552754
[comparative study of nutrient media for detecting shigella in food products]. 197552752
[bacteriological studies of butirosin sulfate, a new aminoglycoside antibiotic (author's transl)]. 197551941
antigenic, antibiotic and phage receptor activity of proteins from sh. boydii 15.the analyzed strain of sh. boydii 15 induced antibodies against the somatic antigen conjugate, as in most shigella. except for the proteinic carrier of the somatic antigen, only a few of the homologous proteinic determinants induced corresponding antibodies; these were illustrated with the help of a serum to proteins from sh. sonnei. the proteins of sh. boydii 15 displayed a wide range of antibiotic activity against shigella, e. coli and salmonella. another aspect concerning the biological activ ...197549990
[improvement of the method of isolation of the causative agents of acute intestinal diseases]. 197548601
[plasmocoagulation of pathogenic enteric bacteria]. 197548600
[study of the virulence of shigella in the reaction with tripaflavin with the aid of kertoconjunctival test]. 197548599
[lysogenicity of shigellae with polyvalent dysentery bacteriophage (according to materials of a hospital for infectious diseases for 1960-1971)]. 197548598
[use of passive hemagglutination inhibition test for diagnosis of sonne dysentery under conditions of practical laboratory]. 197548595
[method of preparation and use of paper bands with levomycetin for isolation of shigella]. 197548593
importance of a new virus in acute sporadic enteritis in a year-long survey of children admitted with acute enteritis to hospital in melbourne, australia, an aetiological agent was found in approximately 75% of patients. during most months of the year a newly described virus belonging to the family reoviridae was detected by electron microscopy of faecal extracts from more than 50% of patients. recovery of this virus reached a peak (73%) during the winter. all age-group up to five years of age were susceptible to infection, which was occasionally f ...197546387
shigellosis in day-care centres.increasing numbers of outbreaks of shigellosis in day-care centres have been reported to the center for disease control since 1972. investigations reveal certain unique epidemiological features of shigellosis in this setting. attack-rates tend to be higher than in outbreaks in primary schools, and epidemiologically these outbreaks resemble those in custodial institutions. person-to-person transmission is the usual mode of spread; secondary spread within households is common, and there may also b ...197546035
[differentiation of shigella sonnei by the method of determination of its enzyme and colicinogenic properties]. 197545980
importance of colicinogeny for the course of acute bacillary dysentery.the development of acute bacillary dysentery was followed in 23 patients involved in two outbreaks and in three sporadic, mutually unrelated cases. repeated cultivations performed at 2-day intervals for 10 days yielded 386 identifiable strains of "opportune intestinal flora". escherichia coli colicinogenic activity is one of the significant factors of gastrointestinal tract protection. the period of shigella excretion is significantly reduced (p less than 0.02) if an appropriate colicinogenic e. ...197944941
[an oral enteritis-vaccine composed of twelve heat inactivated enterobacteriaceae. 1. communication: theoretical and epidemiological considerations (author's transl)].the infectious diseases of the human intestinal tract which are caused by bacteria must be distinguished into two groups on account of their different pathogenesis: the cyclic infections (typhoid fever, parathyphoid fever) and the local infections (cholera, dysentery, salmonella enteritis, dyspepsia coli infections). the local infections of the intestine do not cause a systemic but only a local immunity of the intestinal mucosa. it is necessary therefore to induce local immunity as active immuno ...197944783
[comparative study of interferon production in mice with the graft versus host reaction].mice with graft versus host reaction (gvhr) show a decreased production of serum interferon and that produced by the bone marrow and spleen cells and blood leukocytes in vitro upon inoculation with newcastle disease virus. interferon induction with lipopolysaccharide of flexner bacteria resulted in activation of production of serum interferon and that induced in spleen cell and blood leukocyte suspensions. serum interferon production after administration of poly(i) . poly(c) was similar in mice ...197834929
diagnosis and treatment: drugs five years later. amoxicillin.amoxicillin is an aminopenicillin available in the united states only for oral use. it has an antibacterial activity and spectrum similar to that of ampicillin and is destroyed by gram-positive and gram-negative beta-lactamases. it is more active against enterococci and salmonellae than ampicillin, but less active against shigella. it is better absorbed than ampicillin from the gastrointestinal tract with blood levels two to two and one half times those of ampicillin. amoxicillin is an excellent ...197934342
[role of volatile fatty acids and ph of the gastrointestinal contents in shigella infection in starved monkeys (author's transl)]. 197830852
volatile fatty acids and ph in the gastrointestinal contents of normal and shigella-infected monkeys. 197827663
effect of ph upon the activity of cefoxitin. 197723373
the ability of enteric bacteria to catalyze the covalent binding of bile acids and cholesterol to dna and their in ability to metabolize benzo(a)pyrene to a binding product and to known metabolites.the capacity of enteric bacteria (e. coli, salmonella, pseudomonas, shigella and klebsiella) to catalyze the covalent binding of benzo(a)pyrene (bp), cholic acid, deoxycholic acid and cholesterol was investigated. in general, these bacteria were incapable of activating bp to a covalently bound product with calf thymus dna. metabolism studies of bp by fluorometric assay failed to indicate any accumulation of bp-3-hydroxy in the incubation medium. detailed metabolic investigation with high-pressur ...197719150
detection of salmonella antigens in the neutralization reaction (anr). 197717975
the unusual case of isolation of the sonne dysentery agent from carious teeth. 197715383
new sugars from antigenic lipopolysaccharides of bacteria: identification and synthesis of 3-o-(r)-1-carboxyethyl-l-rhamnose, an acidic component of shigella dysenteriae type 5 lipopolysaccharide.a new acidic sugar, 3-o-(r)-1-carboxyethyl-l-rhamnose (1), has been identified as a constituent of the o-antigenic lipopolysaccharide of sh. dysenteriae type 5. the structure of 1 has been established by physico-chemical methods and by synthesis. alkylation of methyl 2,5-di-o-benzyl-alpha-l-rhamnofuranoside (6) with (s)- or (r)-2-chloropropionic acids, followed by removal of the protecting groups, afforded 3-o-(r)-1-carboxyethyl-l-rhamnose (9) and 3-o-(s)-1-carboxyethyl-l-rhamnose (10), respecti ...197612866
comparative in vitro studies of cinoxacin, nalidixic acid, and oxolinic acid.cinoxacin and nalidixic acid were found to be similar in in vitro activity against 138 shigella isolates and somewhat less active than oxolinic acid on a weight basis. cross-resistance developed when 10 shigellae were transferred on increasing amounts of the respective agent contained in mueller-hinton agar. plate dilution studies of the effect of changes in agar ph on the minimum inhibitory concentration revealed that the antibacterial activity increased with decreasing ph. protein binding inve ...197612717
rapid diagnosis of enterobacteriaceae. i. detection of bacterial glycosidases.the paper describes a number of tests for the rapid detection of glycosidases including alpha-glucosidase, beta-glucosidase, beta-glucuronidase, beta-xylosidase and alpha-fucosidase. the methods use heavy suspensions of viable but non-multiplying bacteria in a buffered solution of a chromogenic substrate. the results of the tests are readable within 4 h. the application of these tests to a collection of 633 strains of enterobacteriaceae and vibrionaceae demonstrates that some of the tests may be ...19769774
significance of starter cultures for raw sausage aging in view of food and nutrition hygiene.the authors studied the effects of liquid starter cultures on the survival of pathogenic germs. it was found that the foreign bacteria tested differed in growth limitation which is obviously dependent on acidity and the amount of lactic acid produced by fermentation. since the pathogenic bacteria differ in the ability to survive, it is imperative to observe strict hygienic measures in preparing starter cultures and to use absolutely sterile monocultures for raw sausage ageing.19768718
somatic antigens of shigella. structural investigation on the o-specific polysaccharide chain of shigella dysenteriae type 1 lipopolysaccharide.the o-specific polysaccharide obtained from the lipopolysaccharide of shigella dysenteriae type 1 (shigella shiga) by mild acid hydrolysis followed by fractionation on sephadex g-50 was found to be identical to that desribed by morgan's group and was composed of l-rhamnose, d-galactose and n-acetyl-d-glycosamine in a ratio 2:1:1. on the basis of methylation analysis data the polysaccharide was proved to be a linear chain of monosaccharide residues in pyranose forms substituted at position 3, exc ...19768314
kinetic properties of serratia marcescens adenosine 5'-diphosphate glucose pyrophosphorylase.the regulatory properties of partially purified adenosine 5'-diphosphate-(adp) glucose pyrophosphorylase from two serratia marcescens strains (atcc 274 and atcc 15365) have been studied. slight or negligible activation by fructose-p2, pyridoxal-phosphate, or reduced nicotinamide adenine dinucleotide phosphate (nadph) was observed. these compounds were previously shown to be potent activators of the adpglucose pyrophosphorylases from the enterics, salmonella typhimurium, enterobacter aerogenes, e ...19766432
selective enrichment of shigella in the presence of escherichia coli by use of 4-chloro-2-cyclopentylphenyl beta-d-galactopyranoside.a procedure involving the use of citrate-buffered lactose broth (ph 6.5) containing an analogue of a beta-galactoside (4-chloro-2-cyclopentylphenyl beta-d-galactopyranoside) has been developed for the enrichment of shigella in competition with a 100-fold higher population of escherichia coli. the system makes use of the beta-galactosidase activity of e. coli which hydrolyzes the phenolic derivative of beta-galactoside to galactose and an aglycone moiety (4-chloro-2-cyclopentylphenol) which is to ...19766138
bacteriological studies on cefatrizine (s-640 p) (author's transl). 19764635
lactulose therapy in shigella carrier state and acute dysentery.antibiotic-resistant shigella are increasingly prevalent. lactulose, a non-absorbable disaccharide, was investigated as an alternative therapy for shigella infection on the hypothesis that the short-chain fatty acids (inhibitory to shigella) resulting from metabolism of lactulose by normal colonic flora would diminish shigella excretion. a long-term antibiotic-refractory carrier (large bowel) excreting 10(4) to 10(7)shigella sonnei/g of feces was given two courses of lactulose (of 24 and 16 days ...19752098
use of lincomycin, methicillin and ristomycin in the nutrient media for isolating pathogenic intestinal microorganisms.elective-differentiating solid nutrient media for simultaneous isolation of vibrioes, salmonella and shigella were developed. antibiotics active against grampostive microflora and dry bile salts inhibiting the growth of proteus were used as the inhibitors of the growth of the accompanying microflora. the medium was lincomycin and the bile salts may be prepared in a dry form.19752096
past and present aspects of diarrheal disease in childhood. clinical study and treatment (author's transl).the etiologic and pathophysiologic findings described in the first part of this paper have important consequences: the recognition of the specific etiology of diarrhea requires new laboratory methods: most of these, however, are technically easy to perform and do not require a large laboratory. a long-ranging consequence of this changed concept is a well-founded modification of therapy. the most important discovery was, that in a well balanced glucose electrolyte solution sodium and glucose enha ...19751346
ph stability of sugar media in the process of determining shigella sonnei biotypes. 1975876
the cathode bound group antigen of dysentery-provoking escherichieae (author's transl).antigens from disrupted cells of dysentery-provoking and of non-enteropathogenic escherichieae were submitted to immunoelectrophoresis on cellulose acetate stripes at ph 8.0. among 6 immune sera produced for this purpose by immunizing rabbits against desintegrated dysentery bacteria, only one contained a precipitine reacting with an antigen similar to the "generic antigen" of belaya. this - at ph 8.0 - cathode-bound group antigen (kga) could not only be found in virulent but also in 5 attenuated ...1975873
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