microbiological methods. methodology for enteropathogenic escherichia coli.pathogenic biotypes of escherichia coli grow poorly at temperatures greatly different from that of the host. percentages quantitatively recovered at 42.0, 44.0, 44.5, and 45.5 degrees c in lauryl tryptose broth were 100, 76, 76, and 42, respectively. corresponding values for 175 strains of varied origin were 98, 89, 82, and 65%. maximal growth temperature is dependent upon medium. lauryl tryptose and elevated coliform broths were equivalent in the recovery of small inocula (100 cells/ml) at 41.5 ...19751095543
[antibiosis shown by a strain of byssochlamys nivea westling, 1909. iii. chromatographic characteristics and isolation of the active fraction].early papers revealed that culture filtrate of byssochlamys nivea has antibiotic properties. active filtrate of b. nivea is extracted with ethyl acetate. paper chromatography is carried out on whatman no. 1; thin-layer chromatography is carried out on kieselgel; visualization with bioautographic technique reveals a single spot. ethyl acetate extract is passed through silicic acid-celite column. each isolated fraction is tested for antibiotic activity. only one supports this activity; a crystalli ...19751095929
shigella outbreak in amos cottage, hospital for retarded children. 19751095939
diarrhoea: a traveller's tale.travellers' diarrhoea is a result of travelling overseas. proper diagnosis can only be made when a full microbiological investigation is carried. a case due to shigella flexneri is described.19751095981
[hygienic assessment of unboiled milk purchased in the szczecinek county in 1973]. 19751096281
[characteristics of serum immunoglobulins in sonne dysentery in children]. 19751096467
studies on the energetic metabolism of shigella flexneri times escherichia coli hybrids devoid of penetration ability i. glycolysis.the aerobic and anaerobic (in presence of kcn) glycolysis is studied of avirulent hybrids of shigella flexneri according to the keratoconjunctivitis test of sereny with genetic characteristics lac-+kcp-minus devoid of penetration ability. it is established that lac-+kcp-minus hybrids which have lost their ability to penetrate the cells due to replacement of kcp-gene of the chromosome of donor strain e. coli k-12 p4 times, are characterized with a steep decrease of their glycolitic activity in bo ...19751096507
effect of microorganisms isolated from the upper gut of malnourished children on intestinal sugar absorption in vivo.the effect of microorganisms isolated from the upper gastrointestinal tract of malnourished children on intestinal sugar absorption was studied in rats in vivo. pure cultures of organisms were grown overnight in a nutrient broth and the resultant supernatant fluid which contained microorganisms in similar numbers to those found in the patients was used as the basic solution for jejunal perfusions which were done in anesthetized adult wistar rats. the substrate used was arbutin (p-hydroxphenyl-be ...19751096588
comparative efficacy of cephalexin and ampicillin for shigellosis and other types of acute diarrhea in infants and children.most ampicillin-resistant shigella are susceptible to cephalexin. randomized treatment with cephalexin or ampicillin was given to 154 infants and children with acute diarrhea. rectal swab cultures revealed shigella in 42%, salmonella in 6%, enteropathogenic escherichia coli in 2%, and no pathogen in 50%. cephalexin failed to eradicate shigella after 5 days of treatment in 76% of patients as contrasted with 28% of ampicillin-treated patients with susceptible organisms. shigella persisted in 78% o ...19751096800
proceedings: dissociation of bacterial pyrexia from prostaglandin e activity. 19751097014
[physical and chemical properties of tryptophenases of five species of enterobacteriaceae].the molecular weight, sedimentation coefficient, and amino acids composition were determined on five tryptophanases (tpases) from escherichia coli b and e. aurescens, shigella alkalescens, and proteus vulgaris and p. morganii. these tpases have identical sedimentation profile and coefficient (9.6 s), and the same molecular weight (220 000). each enzyme is constituted of four identical subunits having a molecular weight of 55 000. the amino acids composition of these tpases is very similar, with ...19751097068
[differentiation of tryptophanases of five species of enterobacteriaceae by sulfhydryl groups].we have studied the sulfhydryl groups (-sh) on the tryptophanases (tpases) from escherichia coli b. and e. aurescens, shigella alkalescens, and proteus vulgaris and p. morganii. the coli group and the p. morganii apo tpases have 20 -sh groups per mole of enzyme, where as p. vulgaris apotpase has 16. in coli group tpases, there are 16 -sh groups on the mole surface and they are all implicated in the activity and the enzyme-substrate bond. proteus morganii tpase has 8 surface -sh groups, 4 of whic ...19751097069
selective cleavage of glycosidic linkages: studies with the o-specific polysaccharide from shigella dysenteriae type 3.treatment of the o-specific polysaccharide from shigella dysenteriae type 3 with hydrazine in the presence of hydrazine sulphate resulted in quantitative n-deacetylation with the formation of a modified polysaccharide containing free amino groups. oxidation of the modified polysaccharide with periodate did not destroy the 2-amino-2-deoxygalactose residues, thus indicating that they were substituted at position 3. acid hydrolysis of the modified polysaccharide afforded 3-o-(2-amino-2-deoxy-beta ...19751097111
shigellosis in a neonate: successful treatment with an oral elemental diet. 19751097146
comparative in vitro acitvity of chloramphenicol and thiamphenicol on common aerobic and anaerobic gram-negative bacilli(salmonella and shigella excluded).the antibacterial activity of thiamphenicol was compared to that of chloramphenicol against 313 strains of gram-negative bacilli isolated from various clinical specimens. these two antiboitics were equally active against the 106 isolates of haemophilus mic equals 0.1 minus 1.56 mu g/ml) and against 40 strains of bacteroides fragilis (almost all strains being inhibited by 12.5 mug/ml of the two drugs). in contrast, when compared with chloramphenicol, 2-16 times as much of thiamphenicol was requir ...19751097209
shigella strains associated with infantile diarrhea in the kaohsiung 1969 and 1974, 375 shigella strains were isolated in the kaohsiung area, and their serotypes and in vitro susceptibility to antimicorbial agents were studied. in 1969, 249 (16.2%) strains were isolated from 1,476 specimens collected from infantile and young children dirrheal cases, whereas 126 strains (11.6%) were isolated from 1,086 specimens in 1969. the isolation rates were higher in may (21.2%), october (20.8%), april (20.6%), august (19.7), november (18.8%) and january (18.5%), than in o ...19751097211
antibiotic resistance of escherichia coli from non-hospital environments. i. frequency and determinants of ampicllin resistance. 19751097362
chronic vulvovaginitis in children due to shigella flexneri.although previous reports have implicated shigella flexneri in resistant or chronic cases of vulvovaginitis in children, no authors have described the clinical findings of this condition. the report presents four cases of persistent vulvovaginitis in prepubertal indian girls from different reservation communities in arizona. s. flexneri was isolated in pure culture from the vaginal discharge of each patient. all four cases were characterized by a prolonged vaginitis with a bloody, purulent disc ...19751098002
studies on the energetic metabolism of shigella flexneri and escherichia coli hybrids devoid of penetration ability. ii. oxygen uptake and dehydrogenase activity.oxygen uptake and dehydrogenase activity are studied with lac+ kcpminus hybrids of shigella flexneri, being avirulent to the eyes of guinea pigs, because of having lost ability for penetration in epithelial cells. these strains were obtained by hybridization of the virulent strain shigella flexneri 222 5 a with the hfr strain escherichia coli k-12 p4x. it is established that lac+ kcp minus hybrids are characterized by significant decrease of respiratory activity. this decrease is outlined as a t ...19751098322
[the anodic-moving thermolabile antigen (ata) of gram-negative bacteria: its occurrence in rough mutants of shigella flexneri and escherichia coli(author's transl)].the thermolabile antigen (ata) moving to the anode, discovered by us (9) and also described by larsson et al. (8), could be detected in ra-, rb-, re- and rd-mutants of sh. flexneri and, in smaller amounts, in rd- and re- mutants of e. coli o8:k27:h. so it proves to be different from kunin's common antigen (7). our results lead to the conclusion that ata is located in a more inner part of the bacterial cell wall and is fixed by noncovalent linkages.19751098323
[dynamics of dysenteric diseases, caused by sh. sonnei of different enzymatic and conditional colicinogenotypes, in kharkov in 1965-1972].results of studying the enzymatic (by maltose, rhamnose and xylose) properties of 9277 freshly-located sh. sonnei strains isolated in kharkov in 1965-1972 showed that they were referred to 14 enzymatic types (a, b, c, d, e, f, g, k, l, m, n, o, p, q). the strains under study were additionally--within the range of each of the enzymatic type-divided into 8 conditioned coli phi,cinogen types (i-viii) by their sensitivity to colicines of the indicator e. coli, k12 and b cultures. subdivision of the ...19751098335
enteroinvasive and enterotoxigenic escherichia coli. occurrence in acute diarrhea of infants and children.the abilities of escherichia coli to induce diarrhea by enterotoxin production and by intestinal cell penetration have not been explored simultaneously in children with diarrheal disease. in this study, we investigated 36 infants and children with acute diarrhea and 17 healthy controls. from each patient's rectal swab culture, e coli colonies were tested for enterotoxin production by intragastric inoculation in suckling mice and screened for cell penetration with hep-2 cells. colonies showing in ...19751098446
bactericidal and opsonic activities of normal sheep serum against gram-negative bacteria.the bactericidal and opsonic activities of normal sheep serum against 9 smooth and 4 rough strains of gram-negative bacteria were measured. three smooth strains-escherichia coli 3662, salmonella typhimurium, and salmonella gallinarum-were resistant to the bactericidal action of normal sheep serum with or without complement. six smooth strains-e coli o78:k80(b), salmonella arizonae, proteus inconstans, klebsiella pneumoniae, salmonella stanley, and salmonella abortus-equi-were moderately sensitiv ...19751098532
[antigenic relationships between "levinea amalonatica" and "shigella dysenteriae" and "boydii" (author's transl)].twenty-eight strains among thirty-five levinea amalonatica show o antigenic relationships with shigella as follows: with s. dysenteriae 3 (5 strains), s. dysenteriae 4 (1 strain), s. dysenteriae 5 (3 strains), s. dysenteriae 7 (11 strains), s. boydii 6 (7 strains), and with both s. boydii 15 and s. dysenteriae 8 (1 strain). these strains do not induce purulent keratogenous conjunctivitis in guinea pigs as shigella do.19751098541
[survival of microorganisms of the intestinal group and staphylococcus aureus on synthetic polymeric materials]. 19751098966
an outbreak of dysentery caused by shigella dysenteriae type 1 on a coral island in the bay of epidemic of severe dysentery occurred on st. martin island in the bay of bengal at a time when the island, with a population of 1,318, was isolated from the mainland because of the monsoon season. there were 434 cases and 28 deaths within a three-month period. mortality (2%) was limited to the very young and the elderly. shigella dysenteriae type 1 (shiga bacillus) was isolated from 12 of 65 rectal swab specimens. all strains gave similar biochemical reactions and were resistant to tetracycli ...19751099145
colicin types of shigella sonnei in relation to antibiotic resistance.colicin typing of 436 strains of shigella sonnei isolated in dallas during a 10-year period was performed to determine whether resistance to ampicillin was associated with a single strain or was widespread among all s. sonnei types. one hundred ninety-three strains collected during a period of eight and one-half years when ampicillin resistance was 1.6% were available for retrospective review. during the last one and one-half years of study, 20.6% of the 243 strains that represented almost all ...19751099149
specific antibodies and heterologous antigensin monofactor sera.a concentrated monofactor serum 9 (wellcome lot k 8028) precipitated against proteins from 2 different strains of salmonella enteritidis and from a strain of s. dublin, as well as against the proteinic moiety of the somatic antigen from s. typhi 0 901; no polysaccharide from the strains, sharing factor 9, precipitated against this serum. a concentrated monofactor serum 20 (wellcome lot k 6733) precipitated against the proteins from s. emek (8, 20), but not against its polysaccharide. a monofacto ...19751099401
principal component analysis of infraspecific variation in certain types of ecological investigations it may be desirable to investigate infraspecific variation in bacteria. principal component analysis is demonstrated to be satisfactory for this purpose. hypothetical bacterial populations were used to show that such analysis can be used to compare collections of bacterial isolates taken at different times or from different sources. alternatively, given n isolates, whether they represent a single bacterial population can be determined. the method is ...19751099988
surveillance of some infectious diseases among aircrew personnel in southeast asia.a 2-year analysis of specimens among aircrew personnel in southeast asia is reported. stool specimens were examined for intestinal parasites and enteric bacteria, blood smears for blood parasites, and sera tested for transaminases (sgot) and antibodies to entamoeba histolytica, toxoplasma gondii, chikungunya and japanese encephalitis viruses, rickettsia tsutsugamushi, leptospira sp. and pseudomonas pseudomallei. one to four specimens each were obtained from 537 adult males and 20 females. there ...19751100040
[prevalence of biochemical variants of shigella flexneri 6 in poland]. 19751101312
complement-mediated bactericidal system: evidence for a new pathway of complement action.the early components of human complement (c1, c4, and c2) plus certain serum euglobulins will kill pathogenic strains of shigella sonnei. serum from patients with hereditary c3 deficiencies and specific antiserums to c3, c5, and c6 were utilized to demonstrate the absence of requirements for late-acting complement components in this unusual bactericidal system.19751101380
epidemiologic investigation of an outbreak of shiga bacillus dysentery in an island epidemic of dysentery broke out in st. martin island during may through july 1973. the epidemic was caused by shigella dysenteriae type 1. the dysentery could not be controlled by conventional antibiotics and other antidysenteric drugs. the average attack rate was 32.9%. the age specific attack rate was highest in the age group 1-4 years (52.2%). the attack rates were higher in smaller families. the rates were not greatly different amongst people using different sources of water. the overall ...19751101385
[sensitivity of bacterial flora to antibiotics according to data from a clinical hospital].antibiotic sensitivity of e. coli o-124, salmonella and shigella sonnei isolated from patients during 1968-1972 was studied with the disc method. increase in the resistance levels to monomycin and neomycin in e. coli o-124 was observed. sensitivity of salmonella and shigella sonnei to some antibiotics did not practically change.19751101809
hemagglutination test in 150 children with diarrhoea and 100 normal children. 19751102449
some immunochemical and chemical aspects of s and r shigella dysenteriae 1 endotoxins.endotoxins of s and r forms of shigella dysenteriae 1 were prepared by nacl-na citrate extraction, purified by gel chromatography on sephadex g 200 and on sepharose 4b and subjected to immunochemical and chemical analysis. the toxins contained 25--30% of lipids, 40--50% of carbohydrates and 14--24% of protein. the lipid and protein moieties of the lipopolysaccharide-protein complexes exhibited no significant difference, whereas the sugar moieties differed markedly (both qualitatively and quantit ...19751102598
superinfection immunity and prophage repression in phage p1. ii. mapping of the immunity-difference and ampicillin-resistance loci of p1 and phi amp. 19751103441
selective cleavage of glycosidic linkages: studies with the polysaccharide component of shigella dysenteriae type 6 lipopolysaccharide.the polysaccharide component obtained from the lipopolysaccharide of shigella dysenteriae type 6 was subjected to milk hydrolysis with acid, and the products were fractionated on sephadex g-50. an acidic hexosaminoglycan and a core oligosaccharide fraction were obtained, the former containing d-glucose, d-galactose, 2-acetamido-2-deoxy-d-galactose (in the ratios 1:1:1), and an unidentified acidic component (x). the hexosaminoglycan was n-deacetylated and then hydrolysed and deaminated to give 3- ...19751104165
r-factors in urinary tract infection. 19751104976
epidemiological and bacteriological studies on an outbreak of sh. sonnei. 19751104983
[biological peculiarities of the etiology in connection with various clinical manifestations of sonne dysentery in children]. 19751105369
[certain cytochemical indicators of neutrophils in acute dysentery sonnei in children]. 19751105382
[occurrence of the cathode-bound group antigen of the dysentery bacilli in escherichieae types of intermediate character (author's transl)].protoplasmatic antigens of 28 strains of 27 escherichieae types not accepted as shigellae have been examined for the occurrence of a cathode-bound group antigen (kga). from the tested cultures of non-dysentery-provoking types e. coli o28 e1073(z) taylor, e. coli o32, e. coli o115 (27w), alkalescens-dispar o1, a.-d. o1 var. koji, a.-d. o2, a.-d. o3, a.-d. o4, a.-d. o5, a.-d. o6, a.-d. o7, and the intermediate serotypes h62 and 6275-52 no one possessed kga, and likewise one of the two examined e. ...19751106061
typing of shigella sonnei colicins by means of specific enlarged and perfected scheme of typing of shigella sonnei colicins is based on the knowledge of types of colicins produced by abbott's types as well as by newly discovered and determined colicin types. a set of specific indicators prepared from e. coli k13 hfrr, e. coli c6 and shigella sonnei by a selection of suitable mutants and recombinations was used for the typing. seven new colicin types were determined on typing shigellae found in the field. type 9a produces colicins e3, ia, type 16 p ...19751106068
[comparative study of the preventive properties of sera obtained by immunization with shigella flexneri strains of varying degrees of virulence]. 19751106084
[immune serum globulins in acute dysentery in children]. 19751106085
[study of the sensitivity of shigella to ectericide]. 19751106086
[study of the virulence of shigella sonnei isolated from persons with different forms of dysenteric infection].possible ranges of virulence of sh. sonnei strains isolated from the patients and carriers in the foci of sonne dysentery, supposedly with the same source of infection, were studied. sh. sonnei strains isolated from carriers proved to be less virulent than the strains isolated from patients with the clinical manifestation of the infection. a hypothesis was put forward explaining some aspects of carrier state in sonne dysentery, which most probably was explained by the refracterity of the macroor ...19751106091
[intracellular parasitism of shigella in patients with dysentery].bioptic specimens of the mucosa of the distal portion of the colon in patients with dysentery, caused by flexner and sonne shigellas, were studied by luminescent-serological and electron-microscopy methods. in the cells with unchanged ultrastructure no shigellas were detected. intracellular localization of shigellas was observed only in cases of deep irreversible alterations in the ultrastructure of the cells.19751106366
[pathogenicity factors of gramnegative enteropathogenic bacteria and pathogenesis of intestinal diseases (author's transl)].enterotoxins as produced by the majority of enteropathogenic bacteria play a central role in the pathogenesis of cholera and e. coli enteritis but are obviously of little significance as to the development of salmonella enteritis and dysentery. pathology of salmonella enteritis and dysentery results from multiplication of bacteria within the bowel wall and from the damage of tissue caused thereby. the lipopolysaccharides (endotoxins) of invasive enteropathogenic bacteria do not possess the chara ...19751106437
colicin typing of shigella sonnei isolated in northern taiwan.eighty-eight strains of shigella sonnei isolated in northern taiwan during the period of 1970-1974, were studied for their colicin types by a modified method of abbott and shannon. as a result, 67(76%) typable and 21(24%) non-typable strains were found. the prevalent colicin types of s. sonnei in northern taiwan were types 15(52%) and 6(15%), followed by types 8(3%), 11(3%) and 12(3%). other types were not found.19751106960
[morphologic characteristics of lysogenic phages with conversion activity in shigella flexneri].the morphology of three lysogenic bacteriophages which induce antigenic conversion in various shigella flexneri serotypes (ii and v type antigen and 7.8 group antigen) has been investigated. a quite different morphology appeared with respect to that of salmonella.19751107127
pathogenesis of shigella diarrhea. serum anticytotoxin antibody response produced by toxigenic and nontoxigenic shigella dysenteriae 1.the serum antitoxin response to the cytotoxin contained in preparations of shigella dysenteriae 1 (shiga's bacillus) exotoxin was studied in natural and experimental infections of man. natural infection resulted in the rapid appearance of toxin-neutralizing antibody, which disappeared some time between 9 and 18 mo after infection. experimental infection of human volunteers provided the opportunity to study immunoglobulin class of the antibody in sera obtained serially from 7 to 50 days after inf ...19761107354
observations on environmental contamination in a microbiological laboratory.contamination of a laboratory environment with pathogenic or non-pathogenic micro-organisms may be relevant to safety of technicians and quality of technical performance. two widely separated incidents in 1968 and 1974 initiated a study of aspects of the laboratory environment. water-baths, water of syneresis and portions of salmonella cultures spurting out of the sterilizing flame were examined. the water of water-baths was shown to be contaminated from the fluid cultures incubated in them. thi ...19761107415
[antimicrobial and antineoplastic properties of alkaloids of greater celandine and their thiophosphamide derivatives]. 19751107767
[an analysis of meat products as possible factors responsible for transmission of dysenterial toxic infections].the paper carries experimental data derived from a bacteriological analysis of infected minced meat products for the presence of shigella sonnei and for ascertaining technological stages non-compliance with whose proper course presents the greatest epidemiological hazards. the procedures employed in analyzing infected meat products are shown to be sufficiently sensitive, for they enable one to count shigella dysenteriae in numbers of 102-106/g, depending upon the degree of total bacterial contam ...19751108439
[biochemical and genetic characteristics of enteropathogenic escherichia coli and shigella. v. the biological properties of auxotrophic mutants of shigella flexneri 2a and of escherichia coli serogroup 0124:k72]. 19751108515
[adjuvant action of nonspecific inflammation in immunizing guinea pigs vis the keratoconjunctival sac with shigella sonnei somatic antigens]. 19751108517
[genetic control of the capacity of sh. flexneri to exert a lethal effect on macrophages. ii. mapping of the cyt-determinant on the shigella chromosome].crossing experiments showed independence of the genetic determinants controlling the capacity of sh. flexneri to synthesize the primary s-specific side chains (antigen 3,4) and to produce a lethal action on macrophages cultivated in vitro. cytotoxicity was restored only in transmission to the r-strain of shigellae of the capacity to synthesize the antigenic factor 3,4 from the cyt+, but not from the cyt-- donor of sh. flexneri. the determinant responsible for the synthesis of cytotoxin designate ...19751108529
[biological characteristics of biochemical variants of sh. sonnei]. 19751108532
[characteristics of serological group o144:k? escherichia isolated in acute intestinal diseases].a study of 14 strains of e. coli belonging to serological group o144: k? isolated in clinical dysentery (bacteriologically unconfirmed), from contacts in the foci of clinical dysentery and acute enteric disease of unknown etiology, and also in prophylactic examination, was made. among these cultures there were strains with shigella characteristics. along with the serological type o144: k?: h--described earlier there was determined serological type o144: k?: h4 differing in the enzymatic activity ...19751108539
[preparation and properties of some derivatives of methyl-6-amino-6,8 dideoxy-1-thio-d-erythro-alpha-d-galacto octopyranoside]. 19751108598
changes in the biochemical properties of shigella flexneri la as a result of recombination with escherichia coli k12.the fermentative properties of recombinants obtained after conjugation between e. coli k12 hfec and s. flexneri la lac phe mutant were investigated. the recombinants showed the antigenic structure of s. flexneri but their biochemical characteristics differed from the properties typical for this genus. the recombinants were found to acquire the ability to ferment several carbohydrates not degraded by the s. flexneri recipient strain.19751108604
[shigella in seawater and in an estuary. iii. determination in vitro of the survival time of s. sonnei ycd in seawater]. 19751108731
[prodigiosin in the overall therapy of dysentery and in the prevention of intrahospital viral respiratory infection in children].prodigiozan was tested in complex therapy of children with acute dysentery. comparison of clinical symptoms, specific immunogenesis and child sanation periods from shigella in the child groups treated (80) and non-treated (74) with prodigiozan showed that prodigiozan lowered the rate of intrahospital acute respiratory virus infection, provided more favourable dysentery progression, more tensed specific immunity and the patient clearance from shigella.19751108774
subdividing of phage types of shigella flexneri by means of bacteriocins. 19751108842
serum factors capable of opsonizing shigella for phagocytosis by polymorphonuclear neutrophils.twenty-five shigella strains were tested for their susceptibility to phagocytosis and killing by polymorphonuclear neutrophils (pmn). the studies identified several serum factors that could participate in opsonization. the strains varied remarkably in their susceptibility to killing when heat-stable opsonins were employed, but all strains were killed when exposed to heat-labile opsonins and pmn. the heat-stable opsonin was shown to be igg, whereas igm was ineffective in the absence of complement ...19751132888
the incidence and antibiotic sensitivity of shigella bacteria isolated from newly imported macaque monkeys. 19751137805
ocular responses and the release of prostaglandin-like substances by shigella endotoxin. 19751142255
[influence of tetracycline on the antibiotic sensitivity of aerobic enteric bacteria]. 19751144980
systemic aspirin and indomethacin do not prevent the response of the monkey eye to trauma.the stability of the blood-aqueous barrier of the monkey eye was challenged by three different methods: anterior chamber paracentesis, intravitreal shigella endotoxin, and subconjunctival arachidonic acid. systemic aspirin and indomethacin were ineffective in stabilizing the blood-aqueous barrier in all three of these systems.19751150400
antimicrobial resistance of shigella isolated in new york city in hundred and two shigella sonnei and fourteen s. flexneri strains isolated from individual patients in new york city hospitals were investigated for antibiotic resistance. the s. sonnei showed 60% resistance to ampicillin and 58% to tetracycline and streptomycin. s. flexneri showed no ampicillin resistance but 50% resistance to tetracycline. there were marked differences in resistance of s. sonnei between hospitals which were not explainable by ethnic or socio-economic differences in the pati ...19751155925
[sanitary-bacterial study of the effectiveness of decontamination of the waste water of voroshilovgrad in agricultural field irrigation]. 19751158158
xylose lysine deoxycholate agar: a useful enrichment subculture medium for salmonellae. 19751160817
[selection, among children in early infancy, of patients or carriers of salmonella and shigella, by means of coproculture at the time of admission to the hospital]. 19751160853
comparative evaluation of desoxycholate citrate medium and xylose lysine desoxycholate medium in the isolation of shigellae.studies of the relative efficacies of different modes of transport of fecal specimens intended for culture for the isolation of shigellae have been carried out. when the delay between collection and culture was long, transportation in liquid nitrogen was slightly but not significantly better than transportation on ice. when the delay was short (10 hours or less) transportation at ambient temperature was as satisfactory as that on ice. a comparative study of the efficacies of three media for the ...19751163491
antimicrobial activities of 81.723 hfu, a new pleuromutilin derivative.the new pleuromutilin derivative 81.723 hfu is extremely active against gram-positive organisms such as streptococci, staphylococci, and against mycoplasmas. a number of shigella, klebsiella, and escherichia coli strains were also found to be quite susceptible to this new agent, whereas other gram-negative organisms like pseudomonas aeruginosa, proteus species, and alcaligenes faecalis proved to be naturally resistant to 81.723 hfu. the new compound acts bacteriostatically. bactericidal effects ...19751170807
release of prostaglandin-like substances by shigella endotoxin and its inhibition by non-steroidal anti-inflammatory compounds.1. the effects of intravenous and intraocular injections of shigella endotoxin on the inflammatory response, the release of prostaglandin-like substances into the aqueous humour, and the effects of indomethacin on the inflammatory response were studied in the rabbit eye. 2. both intravenous and intraocular injection of endotoxin released 12.8 ng to 72 ng of prostaglandin-like substances per ml of aqueous humour and increased the permeability of the blood-aqueous barrier as shown by the rise in a ...19751174763
use of bile-esculin agar for rapid differentiation of enterobacteriaceae.bile-esculin agar has been used for several years for the presumptive identification of group d streptococci. all members of the enterobacteriaceae family will also grow on this medium, but only certain ones can hydrolyze esculin to 6,7-dihydroxycoumarin, which reacts with iron to produce a characteristic blackening of the medium. one thousand and six cultures from clinical specimens representing 20 genera were isolated and identified. heavy inocula from fresh pure culture isolates on heart infu ...19751176613
motility-indole-lysine medium for presumptive identification of enteric pathogens of enterobacteriaceae.detection of lysine decarboxylase activity is a useful supplement to reactions on triple sugar-iron (tsi) and urea agars in the initial examination of suspected pathogenic isolates from fecal cultures. owing to the added value of motility and indole production in the differentiation of enteric pathogens, we prepared and evaluated a motility-indole-lysine (mil) medium. the following 890 organisms were tested: 264 shigella, 2 edwardsiella, 182 salmonella enteritidis, 235 s. typhi, 3 arizona, 32 ye ...19751176632
motility-indole-lysine-sulfide medium.a medium designed for the detection of motility, indole, lysine decarboxylase and deaminase reactions, and h2s production was devised and evaluated. results, using 157 strains of enteric pathogens, were in agreement with reference methods. when 300 isolates from fecal cultures were screened using this medium, shigella was easily differentiated from escherichia and more of the proteus species, especially p. morganii, could be eliminated from further study.19751176633
effect of temperature on transport and plating media for enteric pathogens.the effect of wide variations in incubation temperatures and long periods of incubation on transport and enrichment broths and plating media was determined by exhaustive analysis of 132 diarrheal stools for salmonellae and shigellae. homogenized stools were streaked onto eosin methylene blue (emb), salmonella-shigella (ss), and xylose lysine deoxycholate (xld) agar plates, and into saline, cary-blair (cb) transport medium, and selenite f and gram-negative (gn) enrichment broths. incubation tempe ...19751184731
discriminant analysis of antibiotic susceptibility as a means of bacterial identification.this study shows that antibiotic susceptibility data can be used effectively in the presumptive identification of bacteria. using 12 antibiotics and determining the zone sizes for each, 82% of the isolates considered were correctly identified without any other information. if the inability to distinguish between escherichia coli and shigella is disregarded, the percentage of correct identification is 92%. the method involves determining a set of discriminant functions and defining each taxon by ...19751194406
four hour-test for the identification of enterobacteriaceae.a set of 12 rapid biochemical tests--lysinedecarboxylase, ornithinedecarboxylase, beta-galactosidase, urease, hydrogensulphide, indole, acetoin, deoxyribonuclease, esculin, mannitol, raffinose and sorbitol--were selected from an original set of 13 tests and were found to give 98% accurate reactions within 4 hrs of incubation for the identification of bacteria belonging to enterobacteriaceae. this set permits identification on the genus and/or species level for escherichia, shigella, citrobacter, ...19751196260
[shigella in seawater and in an estuary. i. frequency of bacteriophages in polluted water]. 19751211728
[shigella in seawater and in an estuary. ii. tentative inhibition of adsorption of shigaphages and studies of shigella in a selective milieu]. 19751211729
the effect of macrophage hydrolytic enzyme levels on the uptake and degradation of antigen and immune complexes. 19751214262
[shigellosis in the province of seville]. 19751215811
[some controversial questions on the epidemiology of intestinal infections. report i. significance of the dose and the biological properties of the causative agent in the epidemiology and the clinical picture of salmonelloses and dysentery].on the basis of analysis of literature and personal observations and studies the authors explain a number of epidemiological and clinical peculiarities of intestinal infections with various pathogenic doses of the causative agents; under the term pathogenic dose is understood the minimal amount of microbes necessary for the onset of the disease with the natural mechanism of transmission of the infection. regularities of the effect of the pathogenic dose of the causative agent on the epidemic pro ...19751217345
[nutrition and taxonomy of "enterobacteriaceae" and related bacteria. ii. general results and classification (author's transl)].a study of 186 strains belonging to eleven genera of the family enterobacteriaceae and to three genera of the family vibrionaceae has been carried out in order to determine their "versatility" towards 146 organic substrates tested as sole source of carbon and energy. glucose was the only substrate used by all the strains; gluconate and glycerol were used by respectively 184 and 185 strains; 55 substrates were used by no one strains. the 90 substrates which were used by a fraction of the strains ...19751217783
[nutrition and taxonomy of "enterobacteriaceae" and related bacteria. iii. nutritional characters and differentiation of the taxonomic groups (author's transl)].a batch of 186 strains belonging to the families enterobacteriaceae or vibrionaceae has been studied by determination, for each strain, of the "versatility" towards 146 organic substrates tested as sole source of carbon and energy. this study allowed to work out a classification of these strains into the 32 classes which have been previously described. in the present paper the nutritional characters of these classes are reported. on the basis of these characters, the 32 classes may be regrouped ...19751217784
a method for concentrating bacteriophages using polyethylene glycol. 19751217972
[classification and nomenclature of large--sachs dysentery bacteria]. 19751221688
[possibility of using preservatives and media enrichment for the simultaneous isolation of vibrios, shigella and salmonella]. 19751221721
[salmonella and shigella findings in a large city (1968-1972)]. 19751226913
[comparative evaluation of the methods of treatment of acute dysentery]. 19751231016
[hygenic quality of the cream sold by mobile markets in the federal district of mexico. study of 95 samples]. 19751243203
temperature-dependent cultural and biochemical characteristics of rhamnose-positive yersinia enterocolitica.clinical isolates of rhamnose-positive yersinia enterocolitica (y.e.rh+) were compared with typical rhamnose-negative y. enterocolitica (y.e.rh-) and with yersinia pseudotuberculosis. the y.e.rh+ differed from the y.e.rh- and y. pseudotuberculosis in their ability to ferment raffinose and lactose, utilize citrate and in their inability to grow on hektoen enteric agar at 22 or 37 c, on salmonella-shigella agar at 37 c, and scant on xylose-lysine-deoxycholate agar at 37 c. an extensive temperature ...19761254709
salmonellosis and shigellosis in togo (west africa), 1971-1973. i. carrier rates in the rural population.carrier rates of salmonella and shigella in the rural population of togo were investigated between october 1971 and january 1973. a total of 6051 stool specimens from 5075 persons living in 13 rural communities of all regions of togo was examined. each village was investigated twice, i.e. in the dry and rainy season, respectively. the overall carrier rate was 7.2% (salmonella 6.6%, shigella 0.6%). there was no significant difference in carrier rates of males and females. there was a higher preva ...19761258137
drug resistance and distribution of r factors in salmonella strains.drug resistance and the distribution of r factors in salmonella strains were surveyed using 1,980 strains isolated in japan from 1955 to 1973. resistances were mostly restricted to sulfanilamide (sa), tetracycline (tc), and streptomycin (sm), and combinations thereof. the demonstrated frequency of strains resistant to chloramphenicol (cm) was very low as compared with that in escherichia coli and shigella strains. in relation to resistance to tc, cm, sm and sa, the frequency of isolation of sing ...19761259392
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