the cathode bound group antigen of dysentery-provoking escherichieae (author's transl).antigens from disrupted cells of dysentery-provoking and of non-enteropathogenic escherichieae were submitted to immunoelectrophoresis on cellulose acetate stripes at ph 8.0. among 6 immune sera produced for this purpose by immunizing rabbits against desintegrated dysentery bacteria, only one contained a precipitine reacting with an antigen similar to the "generic antigen" of belaya. this - at ph 8.0 - cathode-bound group antigen (kga) could not only be found in virulent but also in 5 attenuated ...1975873
ph stability of sugar media in the process of determining shigella sonnei biotypes. 1975876
past and present aspects of diarrheal disease in childhood. clinical study and treatment (author's transl).the etiologic and pathophysiologic findings described in the first part of this paper have important consequences: the recognition of the specific etiology of diarrhea requires new laboratory methods: most of these, however, are technically easy to perform and do not require a large laboratory. a long-ranging consequence of this changed concept is a well-founded modification of therapy. the most important discovery was, that in a well balanced glucose electrolyte solution sodium and glucose enha ...19751346
use of lincomycin, methicillin and ristomycin in the nutrient media for isolating pathogenic intestinal microorganisms.elective-differentiating solid nutrient media for simultaneous isolation of vibrioes, salmonella and shigella were developed. antibiotics active against grampostive microflora and dry bile salts inhibiting the growth of proteus were used as the inhibitors of the growth of the accompanying microflora. the medium was lincomycin and the bile salts may be prepared in a dry form.19752096
lactulose therapy in shigella carrier state and acute dysentery.antibiotic-resistant shigella are increasingly prevalent. lactulose, a non-absorbable disaccharide, was investigated as an alternative therapy for shigella infection on the hypothesis that the short-chain fatty acids (inhibitory to shigella) resulting from metabolism of lactulose by normal colonic flora would diminish shigella excretion. a long-term antibiotic-refractory carrier (large bowel) excreting 10(4) to 10(7)shigella sonnei/g of feces was given two courses of lactulose (of 24 and 16 days ...19752098
bacteriological studies on cefatrizine (s-640 p) (author's transl). 19764635
selective enrichment of shigella in the presence of escherichia coli by use of 4-chloro-2-cyclopentylphenyl beta-d-galactopyranoside.a procedure involving the use of citrate-buffered lactose broth (ph 6.5) containing an analogue of a beta-galactoside (4-chloro-2-cyclopentylphenyl beta-d-galactopyranoside) has been developed for the enrichment of shigella in competition with a 100-fold higher population of escherichia coli. the system makes use of the beta-galactosidase activity of e. coli which hydrolyzes the phenolic derivative of beta-galactoside to galactose and an aglycone moiety (4-chloro-2-cyclopentylphenol) which is to ...19766138
kinetic properties of serratia marcescens adenosine 5'-diphosphate glucose pyrophosphorylase.the regulatory properties of partially purified adenosine 5'-diphosphate-(adp) glucose pyrophosphorylase from two serratia marcescens strains (atcc 274 and atcc 15365) have been studied. slight or negligible activation by fructose-p2, pyridoxal-phosphate, or reduced nicotinamide adenine dinucleotide phosphate (nadph) was observed. these compounds were previously shown to be potent activators of the adpglucose pyrophosphorylases from the enterics, salmonella typhimurium, enterobacter aerogenes, e ...19766432
somatic antigens of shigella. structural investigation on the o-specific polysaccharide chain of shigella dysenteriae type 1 lipopolysaccharide.the o-specific polysaccharide obtained from the lipopolysaccharide of shigella dysenteriae type 1 (shigella shiga) by mild acid hydrolysis followed by fractionation on sephadex g-50 was found to be identical to that desribed by morgan's group and was composed of l-rhamnose, d-galactose and n-acetyl-d-glycosamine in a ratio 2:1:1. on the basis of methylation analysis data the polysaccharide was proved to be a linear chain of monosaccharide residues in pyranose forms substituted at position 3, exc ...19768314
significance of starter cultures for raw sausage aging in view of food and nutrition hygiene.the authors studied the effects of liquid starter cultures on the survival of pathogenic germs. it was found that the foreign bacteria tested differed in growth limitation which is obviously dependent on acidity and the amount of lactic acid produced by fermentation. since the pathogenic bacteria differ in the ability to survive, it is imperative to observe strict hygienic measures in preparing starter cultures and to use absolutely sterile monocultures for raw sausage ageing.19768718
rapid diagnosis of enterobacteriaceae. i. detection of bacterial glycosidases.the paper describes a number of tests for the rapid detection of glycosidases including alpha-glucosidase, beta-glucosidase, beta-glucuronidase, beta-xylosidase and alpha-fucosidase. the methods use heavy suspensions of viable but non-multiplying bacteria in a buffered solution of a chromogenic substrate. the results of the tests are readable within 4 h. the application of these tests to a collection of 633 strains of enterobacteriaceae and vibrionaceae demonstrates that some of the tests may be ...19769774
comparative in vitro studies of cinoxacin, nalidixic acid, and oxolinic acid.cinoxacin and nalidixic acid were found to be similar in in vitro activity against 138 shigella isolates and somewhat less active than oxolinic acid on a weight basis. cross-resistance developed when 10 shigellae were transferred on increasing amounts of the respective agent contained in mueller-hinton agar. plate dilution studies of the effect of changes in agar ph on the minimum inhibitory concentration revealed that the antibacterial activity increased with decreasing ph. protein binding inve ...197612717
new sugars from antigenic lipopolysaccharides of bacteria: identification and synthesis of 3-o-(r)-1-carboxyethyl-l-rhamnose, an acidic component of shigella dysenteriae type 5 lipopolysaccharide.a new acidic sugar, 3-o-(r)-1-carboxyethyl-l-rhamnose (1), has been identified as a constituent of the o-antigenic lipopolysaccharide of sh. dysenteriae type 5. the structure of 1 has been established by physico-chemical methods and by synthesis. alkylation of methyl 2,5-di-o-benzyl-alpha-l-rhamnofuranoside (6) with (s)- or (r)-2-chloropropionic acids, followed by removal of the protecting groups, afforded 3-o-(r)-1-carboxyethyl-l-rhamnose (9) and 3-o-(s)-1-carboxyethyl-l-rhamnose (10), respecti ...197612866
the unusual case of isolation of the sonne dysentery agent from carious teeth. 197715383
detection of salmonella antigens in the neutralization reaction (anr). 197717975
the ability of enteric bacteria to catalyze the covalent binding of bile acids and cholesterol to dna and their in ability to metabolize benzo(a)pyrene to a binding product and to known metabolites.the capacity of enteric bacteria (e. coli, salmonella, pseudomonas, shigella and klebsiella) to catalyze the covalent binding of benzo(a)pyrene (bp), cholic acid, deoxycholic acid and cholesterol was investigated. in general, these bacteria were incapable of activating bp to a covalently bound product with calf thymus dna. metabolism studies of bp by fluorometric assay failed to indicate any accumulation of bp-3-hydroxy in the incubation medium. detailed metabolic investigation with high-pressur ...197719150
effect of ph upon the activity of cefoxitin. 197723373
volatile fatty acids and ph in the gastrointestinal contents of normal and shigella-infected monkeys. 197827663
[role of volatile fatty acids and ph of the gastrointestinal contents in shigella infection in starved monkeys (author's transl)]. 197830852
diagnosis and treatment: drugs five years later. amoxicillin.amoxicillin is an aminopenicillin available in the united states only for oral use. it has an antibacterial activity and spectrum similar to that of ampicillin and is destroyed by gram-positive and gram-negative beta-lactamases. it is more active against enterococci and salmonellae than ampicillin, but less active against shigella. it is better absorbed than ampicillin from the gastrointestinal tract with blood levels two to two and one half times those of ampicillin. amoxicillin is an excellent ...197934342
[comparative study of interferon production in mice with the graft versus host reaction].mice with graft versus host reaction (gvhr) show a decreased production of serum interferon and that produced by the bone marrow and spleen cells and blood leukocytes in vitro upon inoculation with newcastle disease virus. interferon induction with lipopolysaccharide of flexner bacteria resulted in activation of production of serum interferon and that induced in spleen cell and blood leukocyte suspensions. serum interferon production after administration of poly(i) . poly(c) was similar in mice ...197834929
[an oral enteritis-vaccine composed of twelve heat inactivated enterobacteriaceae. 1. communication: theoretical and epidemiological considerations (author's transl)].the infectious diseases of the human intestinal tract which are caused by bacteria must be distinguished into two groups on account of their different pathogenesis: the cyclic infections (typhoid fever, parathyphoid fever) and the local infections (cholera, dysentery, salmonella enteritis, dyspepsia coli infections). the local infections of the intestine do not cause a systemic but only a local immunity of the intestinal mucosa. it is necessary therefore to induce local immunity as active immuno ...197944783
importance of colicinogeny for the course of acute bacillary dysentery.the development of acute bacillary dysentery was followed in 23 patients involved in two outbreaks and in three sporadic, mutually unrelated cases. repeated cultivations performed at 2-day intervals for 10 days yielded 386 identifiable strains of "opportune intestinal flora". escherichia coli colicinogenic activity is one of the significant factors of gastrointestinal tract protection. the period of shigella excretion is significantly reduced (p less than 0.02) if an appropriate colicinogenic e. ...197944941
[differentiation of shigella sonnei by the method of determination of its enzyme and colicinogenic properties]. 197545980
shigellosis in day-care centres.increasing numbers of outbreaks of shigellosis in day-care centres have been reported to the center for disease control since 1972. investigations reveal certain unique epidemiological features of shigellosis in this setting. attack-rates tend to be higher than in outbreaks in primary schools, and epidemiologically these outbreaks resemble those in custodial institutions. person-to-person transmission is the usual mode of spread; secondary spread within households is common, and there may also b ...197546035
importance of a new virus in acute sporadic enteritis in a year-long survey of children admitted with acute enteritis to hospital in melbourne, australia, an aetiological agent was found in approximately 75% of patients. during most months of the year a newly described virus belonging to the family reoviridae was detected by electron microscopy of faecal extracts from more than 50% of patients. recovery of this virus reached a peak (73%) during the winter. all age-group up to five years of age were susceptible to infection, which was occasionally f ...197546387
[method of preparation and use of paper bands with levomycetin for isolation of shigella]. 197548593
[use of passive hemagglutination inhibition test for diagnosis of sonne dysentery under conditions of practical laboratory]. 197548595
[lysogenicity of shigellae with polyvalent dysentery bacteriophage (according to materials of a hospital for infectious diseases for 1960-1971)]. 197548598
[study of the virulence of shigella in the reaction with tripaflavin with the aid of kertoconjunctival test]. 197548599
[plasmocoagulation of pathogenic enteric bacteria]. 197548600
[improvement of the method of isolation of the causative agents of acute intestinal diseases]. 197548601
antigenic, antibiotic and phage receptor activity of proteins from sh. boydii 15.the analyzed strain of sh. boydii 15 induced antibodies against the somatic antigen conjugate, as in most shigella. except for the proteinic carrier of the somatic antigen, only a few of the homologous proteinic determinants induced corresponding antibodies; these were illustrated with the help of a serum to proteins from sh. sonnei. the proteins of sh. boydii 15 displayed a wide range of antibiotic activity against shigella, e. coli and salmonella. another aspect concerning the biological activ ...197549990
[bacteriological studies of butirosin sulfate, a new aminoglycoside antibiotic (author's transl)]. 197551941
[comparative study of nutrient media for detecting shigella in food products]. 197552752
[procedures for selecting the ingredients to increase nutrient medium electiveness]. 197552754
[beta-galactosidase activity of shigella]. 197554494
[study of the ability of shigella sonnei and flexneri to inactivate antibiotics]. 197554495
[combined use of rivanol in the media with antibiotics in the isolation of shigella]. 197554496
[characteristics of colicinogenotypes in shigella sonnei]. 197554507
[study of the role of type-specific antigen in the process of shigella flexneri interaction with epithelial cell and macrophage cultures].epithelial cells of the hep-2 line infected in parallel by genetically connected strains of sh. flexneri, differing in the capacity to synthesize the type antigen were studied morphologically. the type antigen proved to show no significant influence on the penetration of dysentery bacilli into the cell. a study of the process of phagocytosis of the mentioned strains by a culture of peritoneal macrophages of guinea pig demonstrated that the presence of a type antigen communicated to the bacteria ...197555022
[obtaining purified preparations of individual shigella newcastle k- and o-antigens by using an ultracentrifugation method].a modified method of obtaining the purified k- and o-antigens of sh. newcastle with the use of ultracentrifugation is described. serologically individual k- and o-antigens were obtained and their chemical composition was studied. polysaccharide of the k-antigen consisted of the galacturonic acid, galactose, glucose, rhamnose xylose and hexosamine; it contained no heptoses. the o-antigen contained galactose, glucose, mannose, rhamnose, heptose and hexosamine; in difference to k-antigen was equal ...197555023
the unfortunate role of precedent in bacteriology. i. the main antigens of salmonellae: the proteins.proteins from s. paratyphi b were found to be better immunogens, in rabbits, than the bacteria from which they were extracted. likewise, proteins from s. newport induced antibodies which reacted in agar gel, against proteins from many species of salmonella. the immunogenicity of the bacterial proteins, as well as their many biological activities are ignored because the classic techniques used lose these main antigens of enterobacteriaceae. the contrasting overemphasized role attributed to the li ...197656833
antigenic determinants of murein lipoprotein and its exposure at the surface of enterobacteriaceae.murein lipoprotein from the outer membrane of escherichia coli could be fixed to erythrocytes without pretreatment of the erythrocytes. passive hemagglutination or immune hemolysis could thus be used as sensitive assays to determine antibodies against lipoprotein. in rabbit antisera prepared against whole cells of e. coli, salmonella, arizona, and shigella antibodies against lipoprotein were present. the respective titers were lowest in encapsulated smooth strains and highest in rough mutants. a ...197657056
role of a lipopolysaccharide gene for immunogenicity of the enterobacterial common is known that only certain strains of the family of enterobacteriaceae, notably rough (r) mutants with the type r1 or r4 core, evoked antibodies in high titers against the common enterobacterial antigen (ca) after immunization of rabbits with heated cell suspensions. the present investigation deals with genetic and immunochemical aspects of certain r1 and r4 mutants isolated from escherichia coli 08 and various shigella serotypes which, unexpectedly, do not induce ca antibody formation. immun ...197657114
ultrastructural localization of viral antigens using the unlabeled antibody-enzyme method.employing the unlabeled antibody enzyme method at the ultrastructural level, a comparison was made between preembedding staining and postembedding staining for the detection of viral antigens. the bacteriophage p1 absorbed to the surface of shigella dysenteriae was used as a model system. preembedding staining resulted in the specific deposition of peroxidase-antiperoxidase (pap) complexes as an electron-dense coating around the viral heads. disadvantages of the preembedding staining method incl ...197657192
method for identifying salmonella and shigella directly from the primary isolation plate by coagglutination of protein a-containing staphylococci sensitized with specific antibody.a technique is described that allows presumptive identification of either salmonella or shigella organisms directly upon the original isolation plate, in this case, macconkey agar. this was accomplished by applying a drop of specifically sensitized protein a-containing staphylococcus aureus over a "suspected" colony or several colonies of organisms grown on macconkey agar. the plate is tilted to and fro to allow mixing of the particles with specific antigen that ir readily solubilized from the c ...197657969
[sensitivity of dysentery bacteria to furazolidone and phthalazol according to the materials of medical epidemiological station of odessa-kishinev railroad]. 197658090
a general method for the isolation of non-sense suppressor strains in gram-negative bacteria. 197558594
[localization on the shigella flexneri f6s chromosome of a rfa gene implicated in the biosynthesis of bacterial cell wall lipopolysaccharide]. 197558625
[receptor sites for phages specific for r mutants of shigella flexneri]. 197558628
letter: transfer of ampicillin resistance from shigella dysenteriae type 1 to escherichia coli. 197659229
[genetic control of the synthesis of newcastle shigella type-specific vi antigen].the authors studied the localization of a determinant of the vi type antigen, sh. newcastle being used in the capacity of donors and e. coli k12 as a recipient. the determinant of the vi type-specific antigen was apparently controlled by the chromosomal gene localized in the region of the histidine operon (on the left from it; 45% of linkage with the his marker).197660854
[antigenic composition of different forms of sh. sonnei].data are presented on the study, with the aid of immunophoresis, of the antigenic composition of the i, ii phases and r-forms of sh. sonnei. from 5 to 7 antigens differing by thermoresistance, electrophoretic and diffuse mobility were found in the composition of microbial cells of various forms sh. sonnei. differences between the i phase on the one hand and cells of the ii phase and r-form, on the other hand, consisted both in the quantitative composition of components of their thermostable o-an ...197660855
[determination of the immunodominant monosugars of newcastle shigella surface k-antigen]. 197662466
[electron microscopic study of the i, ii phases and r-forms of sh. sonnei].electron microscopic study of the microbial cells of the i, ii phases and the r-form was carried out. intact cells were examined by negative contrasting, and morphological differences of various bacterial phases were shown: cells of the i phase had a relatively smooth surface, bacteria of the ii phase had a smooth surface, but many cell wall fragments were split from them; the surface of the r-form cells was coarse, folded, and cell wall fragments were split from the majority of bacteria. antige ...197662474
studies on the pathogenesis of enteric infections caused by invasive bacteria.salmonellae, shigellae and some escherichia coli must invade the intestinal epithelial cell and multiply within the mucosa to cause disease. although the bacterial cell most likely possesses several properties essential to this invasive ability, the nature of the cell envelope complex is at present the only characteristic which has been implicated in this process. while a number of pathophysiological events result from invasion, some of our recent efforts have concerned the site and mechanism of ...197662646
sh. dysenteriae serotypes2,4,8-immunochemistry and phage receptor activity.among three analyzed serotypes of shigella dysenteriae, namely, the serotypes 2,4 and 8, the serotype 2 proved to be a strong immunogen in rabbits, inducing anti-polysaccharide antibodies as well as antiprotein antibodies in all the animals. in contrast, the serotypes 4 and 8 were weak immunogens and among the rabbits some have synthesized only anti-proteins while others had antibodies against the somatic conjugate. aside from the somatic antigens, large amounts of proteins were isolated from al ...197663197
[method of intraspecies serotyping of shigella sonnei according to the determinants of antibiotic resistance and their transmissiveness]. 197663635
[microorganisms of the plesiomonas genus and their significance in the etiology of acute diarrheal diseases]. 197664093
colitic arthropathies."colitic arthropathy" is commonly used to describe the arthritis associated with ulcerative colitis or regional enteritis. the term also describes the acute sterile arthritis which infrequently follows infection of the intestinal tract with salmonella, shigella, or yersinia. sterile arthritis is the most common extraintestinal manifestation of whipple's disease, in which an unidentified bacteria is present in greatest concentration in the small bowel. although arthritis and intestinal symptoms a ...197764976
immunogenic dna of shigella sonnei bacteriophage. 197665323
[several allergic reactions under conditions of functioning of an inhibitor of antibody activity in the body].allergization under conditions of functioning an inhibitory factor in the organism was studied by the reaction of leukocytolysis with tsuverkalov's dysenterin and in the reproduction of hypersensitivity of the immediate type on the schultz-dale's model. it was shown that in functioning an inhibitory factor in the organism the degree of leukocytolysis reaction with the dysentery allergen decreased in the immunized animals in parallel with the fall in the serum antibody activity. a depression of t ...197765882
[method of pouring-out of ploskirev's bactoagar with levomycetin in bacteriological diagnosis of dysentery]. 197666412
[method of colicin typing of enterobacteriaceae]. 197666413
[role of several functional groups in the biological activity of allergenic preparations]. 197666777
[study of genetic control of synthesis of type iii and 6th group-specific antigens of flexner shigellae].as revealed in experiments of interspecific and intraspecific crosses, the determinant responsible for the group-specific 6, as well as controling the synthesis of type iii antigens of sh. flexneri were localized near the lac-pro markers on the chromosome. in intraspecies crosses of bacteria of y variant with the donor sh. flexneri 3c strain the percentage of linking of lac+a6+ was 32, and of lac+aiii+ --24. genetic confirmation of the dependence of the function of the a6 (defined as att6) deter ...197768643
[evaluation of a differential-diagnostic medium with the preparation "progress" for bacteriological diagnosis of dysentery]. 197769763
haemolytic anaemia in bacillary dysentery. 197770682
imported shiga dysentery. 197771589
[passive hemolysis test as a method of serological diagnosis of sonne dysentery]. 197774451
investigation of the antigenic composition of the cells and the exotoxin of sh. dysenteriae (shiga) by agar immunoelectrophoresis and polyacrylamide gel disc electrophoresis.using the method of immunoelectrophoresis in agar, we established the presence of 12 soluble antigens in sh. dysenteriae (shiga). we differentiated among them 3 specific antigens situated in the cathodic region. one of them is the somatic o antigen while the other two are thermolabile surface k antigens of these bacteria. k antigens of grigoryev-shiga bacteria differ from the earlier described k antigens of the newcastle and boyd shigellae in their positive electric charge, in greater lability i ...197775222
[increasing the effectiveness of seeding of shigellae]. 197876719
[use of a standard for colicin genotyping]. 197876720
[evaluation test of electroimmunodiffusion as a method of determining proteins in biological substrates]. 197876728
shigellosis and haemolytic uraemic syndrome. 197876973
synthetic disaccharide-protein antigen for production of specific o2 antiserum for immunofluorescence diagnosis of salmonella.antisera from rabbits immunized with the synthetic disaccharide paratose 1 leads to alpha 3 mannose, representive of salmonella o-antigen 2, covalently linked to bovine serum albumin (bsa), were used in indirect immunofluorescence studies for the identification of salmonella serogroup a (o-antigen 1,2,12) bacteria. among 1311 enteric bacteria tested, 497 were salmonella. the anti-paratose 1 leads to alpha 3 mannose-bsa serum identified correctly all the 63 serogroup a strains tested. no positive ...197878659
whipple's disease: case report with immunological studies.a patient with whipple's disease is described whose small bowel biopsy demonstrated antigenicity to rabbit antisera to groups a, b, c, and g streptococci and shigella flexneri, but not to antisera to pneumococcus or shigella sonnei by immunofluorescent techniques. in addition, the patient's lymphocytes responded normally to phytohemagglutinin and to concanavalin a. these studies support the idea what whipple's disease is mediated by a bacteria-like agent which shares certain antigenic similariti ...197878871
[detection of shigella antibodies in the saliva as an additional method of diagnosis of dysentery]. 197879021
[immunoelectrophoretic analysis of the antigenic structure of sh. sonnei].the authors studied the antigenic composition of 105 sh. sonnei strains freshly isolated from patients suffering from acute dysentery and carriers. immunophoregrams of pure s-and r-forms species were obtained. up to 13 antigens differing by electrophoretic and diffusion mobility and immunological specificity were revealed among soluble sh. sonnei antigens the position of common and specific antigens was determined on the immunophoregram. along with the thermostable somatic o-antigen detected at ...197879280
[biology of shigella stutzeri-schmitzii]. 197880487
lipopolysaccharides of shigella flexneri 6 as phage bacteriophages specifically lytic for representative strains of shigella flexneri serotype 6 were studied. cross hemagglutination experiments and chemical analysis of isolated lipopolysaccharides showed that strains 975 and 323 are smooth and strains 288, 551 and 488--rough. lipopolysaccharides isolated from the smooth strains inactivated phages f10 and "a" whereas those from the rough strains demonstrated poor inactivation of specific bacteriophages. the lipopolysaccharides of both the sm ...197880932
[production of antibodies against shigella flexneri antigen types i and ii by means of affinity chromatography]. 197882304
[effective method of sensitization of fixed erythrocytes with immune sera]. 197882647
synovial inflammation in shigella infection. 197985222
[relationship of the structure of specific somatic antigen polysaccharides in shigella dysenteriae bacteriae to the immunochemical properties]. 197985516
immunochemical characteristics of shigella sonnei and serotype 6 shigella flexneri lipopolysaccharides and enterobacterial common antigen.immunochemical studies on shigella sonnei and serotype 6 shigella flexneri 0 antigens (lipopolysaccharides) and enterobacterial common antigen (eca) isolated from shigella sonnei were carried out. oligosaccharide structure of 0-specific chain of serotype 6 shigella fiexneri lipopolysaccharide was defined and beta-l-rhamnosyl-1,3-n-acetyl-d-galactosamin as immunodeterminant of type vi specificity was recognized. the structures of core regions of lipopolysaccharides isolated from r mutants of both ...197886336
[immunogenic complexes of the soluble surface antigens of sh. sonnei].the comparative study of the immunogenic properties of sh. sonnei (phases i and ii) soluble surface antigens obtained by the modified method of aqueous-saline extraction and sh. sonnei (phase i) antigen obtained by boivin's method was made with the use of the keratoconjunctival test in guinea pigs. the protective activity of a high molecular fraction obtained by the fractionation of phase i soluble surface antigens in sepharose 4b was studied. boivin's antigen, when used for immunization in opti ...197987068
simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions.a differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle.197988460
[use of the er1 65m densitometer for quantitative evaluation of electrophoretograms during the study of amino acid decarboxylases in microorganisms]. 197992601
[modified method of obtaining washings of the large intestine for determining coproantibodies and immunoglobulins in the dynamics of acute dysentery].the study of the washings obtained during rectoscopy from the mucous membrane of the large intestine with the use of a special attachment for a rectoscope revealed that this method had a number of advantages when compared with the study of coprofiltrates; in particular, this method allowed to obtain pure secretions of the mucous membrane free of fecal admixtures and to concentrate the gamma globulin fraction. the study of the dynamics of coproantibody secretion in acute dysentery revealed that c ...197992867
antigenic similarity of morphologic type ciii1 bacteriophages.the aim of the study was to demonstrate antigenic differentiation among bacteriophages belonging to the same morphologic type--ciii1 according to krzywy and slopek or a2 according to ackermann. twenty-six bacteriophages which multiplied on various strains of bacteria of the genera escherichia, shigella and klebsiella, were studied. serologic tests were done by the quantitative complement fixation test. immune sera against 5 phi e. coli. d8 sh. flexneri, g35 sh. sonnei and kl7 klebsiella bacterio ...197992976
identification of bacteroides fragilis by indirect immunofluorescence.rabbit antiserum against live or heat-killed bacteroides fragilis nctc 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'b. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the b. fragilis nctc 9343 polysaccharide. when the antiserum was used in indirect immunofluorescence, 97.1% of 244 b. fragilis strains were correctly identified. only 8 of the other 312 bacteroides strains were stained ...197994651
[disinfection of gutta-percha cones]. 197796557
[specific antibodies bound to secretory iga in the sera of patients with intestinal infections].specific iga and siga antibodies were studied in the sera of patients suffering from various intestinal diseases (dysentery, salmonellosis, typhoid fever, chronic typhoid carrier state) and in the sera of healthy persons immunized by parenteral route with typhoid alcohol vaccine. the nature of antibodies was identified in coombs' test, using monospecific antisera to alpha-chain and to the secretory component. iga and siga antibodies were revealed most frequently in the sera of dysentery patients ...197896639
[quarantine and health control of the squirrel monkey (saimiri sciureus) (author's transl)].a total of two hundreds and seventy squirrel monkeys (saimiri sciureus) of both sexes were imported from south america through an animal dealer of the u. s. a. in eight lots from may, 1973 to july, 1974. they were investigated for the presence of the parasites for a maximum of fifteen months including nine weeks of quarantine period from the time of import. the mortality rate before or immediately after arrival at our laboratory reached 56.7% in the first two lots. this, however, could be reduce ...197897090
[prospects for the use of blood substitutes made in the ussr with the expired date of validity].as revealed, blood substitutes--aminopeptide, caseine hydrolysate, and hydrolysine with the expired date of validity could be used as the basis of nutrient media for the cultivation of bacteria. bacterial growth proved to be the optimum in addition to the blood substitutes of a 0.25--0.5% of yeast extract.197897885
[leg ulcer caused by a monkey's bite].35 days following a monkey's bite, the ulcer observed showed total necrosis associated with spotted zones of necrosis due to the deep penetration of the animal's teeth. it was treated like a regular ulcer and healed within seven weeks without complications. one should keep in mind that such ulcers involve infectious episodes due to the transmission of a pathogenic human-type flora through a septic orodental milieu: pathogenic cocci; pasteurella, shigella, salmonella; polio viruses or microbial a ...197899755
[studies of antibody against shigella in bacillary dysentery patients and carriers. report 4. comparison of the results obtained from igg antibody absorption with protein a-sepharose cl-4b and from those with indirect radioimmunoassay (author's transl)]. 1977101599
enterobacterial common antigen: isolation from shigella sonnei, purification and immunochemical the studies presented the effective procedure of isolation and purification of enterobacterial common antigen from shigella sonnei has been elaborated. the method is based on sonification of bacterial suspension in the presence of lysozyme and edta and subsequent extraction of the pellet with boiling water. the crude extract of common antigen was purified by fractionation with ethanol and chromatography on silica gel and sephadex lh-20. the comparison of several extraction procedures of enter ...1978102511
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