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kinetic properties of serratia marcescens adenosine 5'-diphosphate glucose pyrophosphorylase.the regulatory properties of partially purified adenosine 5'-diphosphate-(adp) glucose pyrophosphorylase from two serratia marcescens strains (atcc 274 and atcc 15365) have been studied. slight or negligible activation by fructose-p2, pyridoxal-phosphate, or reduced nicotinamide adenine dinucleotide phosphate (nadph) was observed. these compounds were previously shown to be potent activators of the adpglucose pyrophosphorylases from the enterics, salmonella typhimurium, enterobacter aerogenes, e ...19766432
approach to a practical method for screening and identifying microorganism genera from urine (author's transl).in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ...197611179
cyclic guanosine-3',5'-monophosphate--mediator of the action of the hormaone erythropoietin. 197718327
role of gamma-glutamyl-transpeptidase in the course of viral hepatitis: its comparison with other indices of hepatic function. ii. 197826903
delayed response of ankle jerk time changes in decreasing thyroid function in comparison with the serum cholesterol changes.1. serum cholesterol, pbi and ankle jerk time (ajt) were measured simultaneously in a group of not yet treated patients, where a relatively steady state of thyroid function was expected, and in a treated group, where change to lower thyroid function was supposed (thyrostatic drugs, near total thyroidectomy, radioablation of thyroid). 2. serum cholesterol correlated poorly with pbi (r = --0,4427), better correlation was achieved after logarithmic transformation of pbi values (r = --0,6839). 3. re ...197558781
[use of chromolymphotrast for color lymphography]. 197984768
the humoral and cell-mediated immune responses to somatic and metabolic antigens in rats infected with angiostrongylus cantonensis. 197886211
the phenomenon of differentiation in murine erythroleukemic cells. 197886529
moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules. 197987439
the role of p23,30-bearing human macrophages in antigen-induced t lymphocyte responses. 197988270
antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion. 197989825
alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability.the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ...197989900
the indirect rat mast cell degranulation test reconsidered.the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ...197990655
[erythrocyte diagnostica made from salmonellal phagolysates].for the first time o antigens obtained from phagolysates were proved to be suitable for use as material for the production of highly specific erythrocyte diagnostic preparations. o antigens obtained from salmonella by two methods, i.e. phage disintegration and grasset's method, were subjected to comparative chemical analysis and found to have no essential difference. nevertheless, the sensitizing potency of o antigens obtained from phagolysates were experimentally shown to be 3 times greater tha ...197991283
occurrence of beta 2-microglobulin in mammalian lymphocytes and erythrocytes.cell-associated beta 2-microglobulin (beta 2m) has been studied by quantitative techniques in lymphocytes and erythrocytes of guinea pig, rabbit, rat and man. beta 2m was solubilized by sonication followed by treatment with acid, deoxycholate or thiocyanate, and then determined by radioimmunoassay. the average number of beta 2m molecules per lymphocyte, estimated after acidification, varied between 0.89 x 10(5) and 7.1 x 10(5). rat erythrocytes contained 3.0 x 10(3) molecules per cell, whereas n ...197991519
morphochemic studies on histamine and monoaminooxidase in the liver and studies on histaminolytic activity of guinea pig plasma in the course of sensitization with heterogenic protein. 197991564
on the development of the two components of glycogen synthase phosphatase in foetal rat liver [proceedings]. 197992285
nonspecific light loss and intrinsic dna variation problems associated with feulgen dna cytophotometry.nonspecific light loss by the cell-wall-plus-cytoplasm (cwc) can cause a 50% increase in feulgen absorption units in peanut root-tip nuclei as determined by scanning at 450 nm, whereas this phenomenon is not evident with chicken erythrocytes. a two wavelength scanning method of subtracting nonspecific 450 nm absorption from 550 nm feulgen absorption values eliminated the nonspecific light loss in cwc, however, the two wavelength scanning method is time consuming and somewhat impractical with a r ...197992496
dissociation and exchange of the beta 2-microglobulin subunit of hla-a and hla-b antigens.human histocompatibility antigens hla-a, hla-b, and hla-c are a complex of two noncovalently associated subunits: a heavy chain glycoprotein (alpha) carrying the genetic polymorphism and an invariant light chain, beta 2-microglobulin (beta 2m). upon incubation of papain-solubilized hla with radiolabeled urinary beta 2m, the latter is incorporated into hla, where it substitutes for the preexisting beta 2m that has dissociated from the complex. the association-dissociation equilibrium that governs ...197993282
the effect of crude bursa of fabricius extracts on the humoral immune response and its recovery in bursectomized chickens. 197993554
symposium on uropathology. 197993566
genetic variation in the m antigen of group a streptococci: reassortment of type-specific markers and possible antigenic drift.the phylogenetic relationships of 73 strains of group a streptococcus isolated over a six-year period from a population with a high prevalence of streptococcal infections were analyzed with use of four serotype-specific markers: the serum opacity factor (of), the t antigen, the determinant of the m antigen that precipitates with antibody, and the m antiphagocytic determinant. of inhibition tests divided the strains into five subtypes: a, b, c, d, and e. strains within subtypes a, b, and c shared ...197993611
location of bladder preganglionic neurons within the sacral parasympathetic nucleus of the cat.sacral parasympathetic preganglionic neurons innervating the urinary bladder were labelled by applying horseradish peroxidase (hrp) to branches of the pelvic nerve at the neck of the bladder. bladder preganglionic cells were located primarily in the intermediolateral grey matter of sacral cord segments 1, 2 and 3 and extended from the ventral end of the central canal to the inferior margin of the dorsal horn. dendrites extended into the dorsolateral funiculus, toward the lateral edge of the dors ...197993725
study of effectiveness of bleomycin in rat brain tumor model intravenously and intracerebrally. 197994056
[histochemical studies on the structure and the age changes of descemet's membrane in cattle (author's transl)].descemet's membrane of 15 cattle-corneas, aged 6 month to 13 years was studied by various histochemical and morphometric methods. 3 zones could be differentiated. zone 1 is relatively small and does not show significant changes with age; zone 2 comprises the main part of descemet's membrane, its diameter increased with age. zone 2 is rich in sulfated glycosaminoglycans. zone 3, below the endothelial lining, does not develop before the 3rd and 5th year of life and stains different from zone 2.197994246
the identification of human h-y antigen and testicular transformation induced by its interaction with the receptor site of bovine fetal ovarian cells.beta 2m(-), hla (-) daudi human male burkitt lymphoma cells excreted a group of protein subunits that shared three distinctive characteristics; their conspicuously longer half-lives compared to more hydrophilic daudi excreted proteins, their tendency to form progressively larger polymers by means of interchain disulfide bridges, and the extreme hydrophobicity of these polymers. the plasma membrane of extragonadal somatic cells absorbed 1.2 to 2.8% of these hydrophobic proteins. the unoccupied h- ...197994287
comparison of the antibodies elicited by the individual structural polypeptides of foot-and mouth disease and polio viruses.antibody produced against preparations of vp1, one of the four structural polypeptides of foot-and-mouth disease virus, neutralized the virus and reacted with both full and empty particles in radioimmunoassays (ria). antiserum against vp2 reacted with artificial empty particles of the virus but not with full particles. in contrast, none of the individual polypeptides of poliovirus produced antisera which neutralized the virus nor reacted with it in ria. however, antisera produced with vp1 and vp ...197994352
studies on the role of vasopressin in blood pressure control of spontaneously hypertensive rats with established hypertension (shr, stroke-prone strain).we investigated the role of arginine-vasopressin (avp) in maintaining the blood pressure of spontaneously hypertensive (sh) rats (stroke-prone strain) with established hypertension (22--28 weeks of age). in comparison with normotensive wistar kyoto (wky) rats, plasma avp concentrations of sh rats with benign hypertension (bh) were elevated twofold and in rats with severe or malignant hypertension (s-mh), fourfold. the height of the blood pressure was quantitatively related to plasma avp in both ...197994626
new surgical management in the cleft palate repair and its histological aspects. 197994731
[studies of acid-soluble antigens in several strains of the genus bacillus for orienting purposes (author's transl)].by acid hydrolysis (0.2 n hcl) it was possible to obtain extracts from nonsporulated cells of different strains of 5 species of the genus bacillus which were composed mainly or solely of species-specific antigens. this was the result of experiments using the immunodiffusion technique after having prepared antisera from rabbits with the aid of hcl extracts. cross-reacting antibodies were adsorbed easily. the species-specific reacting antisera revealed the existence of group- or strain-specific an ...197995076
fibrinogen turnover in pregnant rabbits during the first and last thirds of gestation.although thrombin-mediated fibrinogen derivatives have been observed in normal pregnancy, an increased turnover of fibrinogen has been suspected but has not yet been demonstrated. fibrinogen turnover was studied in 14 pregnant and 10 nonpregnant female rabbits by use of purified rabbit 125i-labeled fibrinogen. in the last third of gestation the half life of fibrinogen was shortened by 45% and the fractional catabolic rate increased by 44% in comparison to the values for the first third of gestat ...197899036
evidence for a c-terminal tyrosine residue in human and mouse b-lymphocyte membrane mu chains. 197899661
murine macrophage-lymphocyte interactions: scanning electron microscopic study.light and scanning electron microscopic observations revealed murine macrophage-lymphocyte interactions involving the initial contact of peritoneal, spleen, or thymus lymphocytes with peritoneal macrophage processes or microprocesses followed by clustering of lymphocytes over the central nuclear area of the macrophages. lymphocyte-lymphocyte clustering was not observed in the absence of macrophages. attachment and subsequent clustering appeared not to require the presence of serum or antigen; th ...1978101458
dominant constitutive mutations in malt, the positive regulator gene of the maltose regulon in escherichia coli. 1978101676
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...1979104958
[chemotaxonomic markers of several coryneform bacteria and the "rhodochrous" group].chemotaxonomic characters (the monosaccharide composition and diaminopimelic acid (dap) of cell hydrolysates, the type of lipid lcn-a) were studied with bacteria of the "rhodochrous" complex and the arthrobacter genus isolated from natural substrates as well as with collection strains of the genera arthrobacter, brevibacterium and corynebacterium. if arabinose, galactose and meso-dap were present in cell hydrolysates of these bacteria, they always contained lipid lcn-a. bacteria of the "rhodochr ...1978106219
thyroid function studies in the nephrotic syndrome.total serum and urinary thyroxine (t4), triiodothyronine (t3), and thyroxine-binding globulin (tbg) as well as serum free t4, thyroid-stimulating hormone (tsh), and t3 resin uptake (t3ru) were measured in seven patients with the nephrotic syndrome. the nephrotic syndrome was defined by proteinuria exceeding 3 g/24 h. all patients were clinically euthyroid. most values for total serum t4, free t4, t3, t3ru, tbg, and tsh were within normal limits. however, the mean serum t3 and tbg values were sig ...1979106751
microtubules and the regulation of 3-hydroxy-3-methylglutaryl coenzyme a reductase.cultured c-6 glial cells were utilized to evaluate the effect of antimicrotubular drugs on 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase and cholesterol synthesis. colchicine, colcemid, and vinblastine (1.0 mum) caused a marked reduction in hmg-coa reductase activity and, as a consequence, the rate of cholesterol synthesis in these cells. no effect was observed with lumicolchicine, a mixture of colchicine isomers with no effect on microtubules. the effect of colchicine was apparent w ...1979107160
prolongation of islet allograft survival following in vitro culture (24 degrees c) and a single injection of als.isolated rat islets remain morphologically and functionally intact during a 7-day period of in vitro culture at 24 degrees c. in vitro culture of islets at 24 degrees c for 7 days prior to transplantation, in conjunction with a single injection of antiserum to lymphocytes into the diabetic recipient, results in islet allograft survival of 100 days when the islets are transplanted across a major histocompatibility barrier.1979107588
pituitary-thyroid function in spironolactone treated hypertensive women.four weeks high dose spironolactone treatment (aldactone searle, 100 mg q. i. d.) significantly enhanced the tsh (delta max. 8.5 +/- 4.1 vs. 4.6 +/- 3.1 microunits/ml, p less than 0.05) and t3 (delta max. 32 +/- 27 vs. 11 +/- 16 ng/100 ml, p less than 0.05) responses to an intravenous trh/lh-rh bolus injection in 6 eumenorrhoeic euthyroid hypertensive women, without affecting basal serum tsh, t3 or t4 levels or the basal and stimulated lh, fsh and prolactin values (p greater than 0.10). the mean ...1979107696
experimental transmission of wuchereria bancrofti to monkeys.infective larvae of wuchereria bancrofti from laboratory-raised culex pipiens fatigans and aedes togoi mosquitoes fed on human volunteers in jakarta, indonesia (j strain) and kinmen island, china (k strain) were introduced into taiwan monkeys (macaca cyclopis) by subcutaneous inoculation, by foot puncture, or by permitting infected mosquitoes to feed weekly on the monkeys. some animals were splenectomized and others were treated with varying regimens of immunosuppressants. necropsy was done on m ...1979107818
hallucinogen-induced rotational behavior in rats. 1979111284
inhibitory effect of normal mammalian sera on the rosette formation of human lymphocytes with sheep red blood cells. 1975123840
[immunoprofile of lmif in immunologic deficiency syndromes. ii. mediator production by isolated subpopulations of human lymphocytes]. 1978149303
impaired dopamine function and muscular rigidity induced by 6-aminonicotinamide in rats.in corpus striatum, 6-aminonicotinamide (6-an), 10 mg/kg i.p. lowered the concentration of dopamine and markedly reduced the disappearance of dopamine after synthesis inhibition with alpha-methyl-p-tyrosine. in the dopamine-rich part of the limbic system, the formation of dopa after decarboxylase inhibition with 3-hydroxybenzylhydrazine was decreased. in diencephalon, 6-an altered neither the steady-state level nor the utilization of noradrenaline. the data suggest that the muscular rigidity ind ...1978152412
cyclic adenosine 3':5'-monophosphate and inhibition of deoxyribonucleic acid synthesis in vitro.results are presented which suggest that inhibition of tritiated thymidine incorporation into the deoxyribonucleic acid of bone marrow in vitro by high concentrations of cyclic adenosine 3':5'-monophosphate is pharmacological in nature, and thus apparently unrelated to the physiological actions of camp.1975165140
a simple radioimmunoassay for 3',5', cyclic adenosine monophosphate. 1975165745
studies of quartz cytotoxicity on peritoneal macrophages of guinea pigs pretreated with polyvinylpyridine n-oxide. 1975169129
ultrastructure of human bronchiolo-alveolar cell carcinoma.ultrastructural features were correlated with a series of special staining reactions in eight cases of bronchiolo-alveolar carcinoma. ultrastructural, all tumors were similarly composed of large cells with abundant cytoplasm and small nuclei in close contact with each other. straight membranes or complex interdigitations occurred within adjacent tumor cells, attached to each other by scattered desmosomes. microvilli or cilia abutted from free surfaces of the cells, and were noted in different st ...1975169987
d-galactosamine hepatotoxicity. iv. further studies of the pathogenesis of fatty liver. 1976179848
[changes in the functional state and structure of the adrenals in cc14 poisoning].introduction of cci4 in doses of 0.1 and 0.2 ml/100 g to albino rats reduced the steroid production generated by adrenal sections. in 24 and 48 hours after poisoning with a small dose of cci4 (0.1 ml/100 g) there could be seen an increased concentration of 11-oxycorticosteroids in the plasma. morphological signs of a damage suffered by the gland were observed only in poisoning with a large dose of cci4 (0.2 ml/100 g).1975179852
regulation of pyruvate dehydrogenase in rat heart. mechanism of regulation of proportions of dephosphorylated and phosphorylated enzyme by oxidation of fatty acids and ketone bodies and of effects of diabetes: role of coenzyme a, acetyl-coenzyme a and reduced and oxidized nicotinamide-adenine dinucleotide.the proportion of active (dephosphorylated) pyruvate dehydrogenase in perfused rat heart was decreased by alloxan-diabetes or by perfusion with media containing acetate, n-octanoate or palmitate. the total activity of the dehydrogenase was unchanged. 2. pyruvate (5 or 25mm) or dichloroacetate (1mm) increased the proportion of active (dephosphorylated) pyruvate dehydrogenase in perfused rat heart, presumably by inhibiting the pyruvate dehydrogenase kinase reaction. alloxan-diabetes markedly decre ...1976180974
effectiveness of orally administered bordetella pertussis vaccine in mice.oral administration of killed bordetella pertussis organisms to mice results in increased resistance to an intracerebral infection with virulent b. pertussis cells. the rate of survival is dependent on the dose of antigen. but besides specific systemic immunity, which is persistent over a long period, also transient non-specific resistance is increased. these effects are evidently induced without penetration of bacterial substances into the circulation.1976182602
carbamoylphosphate synthetase i of rat-liver mitochondria. purification, properties, and polypeptide molecular weight. 1978206435
genetic studies of the lac repressor. v. repressors which bind operator more tightly generated by suppression and reversion of nonsense mutations. 1978211238
transcription of endogenous c-type viruses in resting and proliferating tissues of balb/mo mice. 1979228474
the identification of new rna-binding proteins in the escherichia coli ribosome. 1977321250
new culture medium for the presumptive identificaion of candida albicans and cryptococcus neoformans.a new medium composed of tween 80, oxgall, caffeic acid, and davis agar (toc) that provides for the rapid presumptive identification of candida albicans and cryptococcus neoformans is described herein. c. albicans is differentiated from other yeasts by the sequential production of germ tubes and chlamydospores. in a comparison with cormeal agar control plates, there was an increase of chlamydospore-forming strains of c. albicans (97.1% versus 87.2%) and a decrease in the time required for chlamy ...1977321472
differentiation of serratia marcescens and serratia liquefaciens by tests for lipase and phospholipase production.the production of lipase and phospolipase by certain members of the enterobacteriaceae was examined by thin-layer chromatography of resting-cell suspensions incubated with triolein or lecithin. most strains of serratia marcescens produced both enzymes while most strains of serratia liquefaciens exhibited strong lipase but only a minor phospholipase activity. enterobacter spp. (25 strains), klebsiella pneumoniae (20 strains), escherichia coli (15 strains), citrobacter freundii (7 strains) and pro ...1978355631
replacement of pseudouridine in transfer rna by 5-fluorouridine does not affect the ability to stimulate the synthesis of guanosine 5'-triphosphate 3'-diphosphate.the requirement for ribothymidine and pseudouridine in the tpsicg loop of trna for its activity in the ribosome and trna-stimulated synthesis of guanosine 5'-triphosphate 3'-diphosphate (pppgpp) by stringent factor has been tested by the use of a purified trnaphe (883 pmol of phenylalanine incorporated/a260 unit) in which 92% of the pseudouridine, 98% of the ribothymidine, 98% of the dihydrouridine, and 88% of the uridines were substituted by 5-fluorouridine. this trna was quantitatively as acti ...1978356873
infective keratitis in soft contact lens wearers.eight cases of infective keratitis are reported in wearers of soft contact lenses. four of them had normal eyes and were wearing lenses on a continual basis. one was wearing a lens continually for therapeutic reasons. three others were wearing lenses daily or intermittently. the four latter cases were using contaminated lens solutions. two of the continual lens wearers lost vision to the point of blindness. a significant factor in their bad outcome was the fact that both lived in areas remote fr ...1977403931
spinal analgesia and sedation of goats with lignocaine and xylazine.spinal anaesthesia of 20 does undergoing laparotomies was accomplished with epidural injections of 2 per cent lignocaine at a dosage of 1 ml per 4.55 kg in 16 goats. epidural injection resulted in paralysis lasting 198.5 minutes +/- 36.6 minutes. subarachnlid injection resulted in paralysis lasting 180.8 minutes +/- 44.9 minutes. xylazine was given intramuscularly at the dosage of 0.11 mg per kg after posterior paralysis was complete. these treatments resulted in effective analgesia for surgical ...1979516312
[problems of the disease due to mycobacteria other than tubercle bacilli: surgical treatment and indication (author's transl)]. 1979522352
[electoneuromyography in the study of diseases of the neuromuscular system].results of electroneuromyographic examinations of 207 patients with hereditary neuromuscular diseases are presented. patients with neural amyotrophies showed a sharp slowdown of the speed of impulse transmission along the median and the tibial nerves, a lowering of the induced potentials of the muscle and the nerve, and an increase of the m-response. in patients with spinal amyotrophies a slight lowering of the speed of the impulse transmission along the tibial nerves with the transmission speed ...1979525113
inositol as a selective substrate for the growth of klebsiellae and serratiae.a synthetic basal salts medium containing inositol as the sole source for carbon and energy and bromothymol blue as ph indicator, selectively supports the growth of klebsiella pneumoniae and serratia spp. on this medium incubated at 37 degrees c for 24 hr, all cultures of kl. pneumoniae, serratia liquefaciens and s. marcescens form yellow colonies. the medium may be used selectively for the primary isolation of the aforementioned bacteria from faeces and as a differential medium for their isolat ...1976790856
[hbbrnoag as a product of serratia liquefaciens treatment of hbsag-positive plasma]. 1977880596
[taxonomic study of enterobacteria belonging or related to the genus enterobacter (author's transl)].the classification of 175 strains related to the genus enterobacter has been carried out by numerical procedure, with usual biochemical and nutritional characters as shown by the utilization of substrates tested as sole sources of carbon and energy. the taxonomic position of the strains is discussed in relation to the species e. cloacae, e. hafniae, e. aerogenes, e. agglomerans and serratia liquefaciens. by the system of data analysis used (hierarchical agregation method) five principal classes ...19761020871
fowl plague virus adapted to human leukemia cells: interaction with normal human leukocytes and plastic surfaces.an avian influenza a virus which grows well in human leukemic myeloblasts was unable to replicate in normal human leukocytes. the virus adhered during the first hours of incubation to plastic surfaces and to leukocytes and was then released into the supernatant; care should be taken not to confuse this with viral growth.19751063990
proceedings: the effect of tris on the response of various isolated, mammalian preparations to nerve and drug stimulation. 19751151799
proceedings: effects of histamine, 2-methylhistamine and 4-methylhistamine on blood pressure and vascular resistance in the cat. 19751151865
water contents of various regions of the feline nervous system.a systematic analysis of the regional water content of the feline nervous system was made. in general, areas rich in grey matter contained about 10-15% more water than white matter areas. percentage water values were lowest for cranial and peripheral nerves and highest for cerebral cortex, cerebellar cortex, olfactory bulb and other grey matter regions. values for cerebral cortex varied from about 81.5% for suprasplenic gyrus to over 84% for frontal gyrus. it is suggested that the effective conc ...19751214540
[antimicrobic properties of facultative anaerobes isolated from oil-bearing wells]. 19751214635
[effect of x-rays on human adult skin explants]. 19751233920
renaturation of bacteriophage lambda dna. determination of the optimal renaturation conditions using a single-strand-specific dnase and alkaline-sucrose-gradient assay system.reannealed hybrid molecules of wild-type bacteriophage lambda dna were prepared in aqueous solutions of formamide at a variety of nacl concentrations at both room temperature ( 22 degrees c) and 37 degrees c. treatment of the hybrid dna molecules with the single-strand-specific nuclease s1 from aspergillus oryzae followed by alkaline sucrose gradient sedimentation was used to monitor the extent and fidelity of hybridization. the optimal renaturation conditions at room temperature were found to b ...19761248479
the lecithin: cholesterol acyltransferase reaction, lysolecithin and red cell ageing in blood stored under normal transfusion service conditions.the lecithin:cholesterol acyltransferase (lcat) activity and lysolecithin content of human blood stored under standard blood transfusion service conditions at 4 degrees c for 6 weeks has been investigated. cooling the blood to 4 degrees c rapidly inactivates the lcat reaction, but the enzyme is not denatured during storage under these conditions. citrate in the anticoagulant did not activate the lcat reaction in freshly-taken whole blood. the total phospholipid and total lysolecithin content of ...19761251568
dna relatedness among species of enterobacter and serratia.species of enterobacter and serratia were examined for deoxyribonucleic acid relatedness to klebsielleae, to atypical erwiniae, and to other members of enterobacteriaceae. deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. strains of enterobacter cloacae formed two separate hybridization groups that correlate with the presence or absence of yellow pigment. pigmented e. cloacae were 75-100% related, but they were only 40-50% re ...19761260520
the clinical significance of in vitro cytomegalovirus susceptibility to antiviral drugs. 19921317603
phenotypic and functional studies on ocular t cells during herpetic infections of the eye.herpetic stromal keratitis an inflammatory disease of the eye resulting from herpes simplex virus type 1 infection, is a common cause of blindness. the disease is generally considered to represent an immunopathologic response, but the exact mechanism remains in doubt and is subject to debate. we have investigated the nature of inflammatory cells in the eye and have isolated ocular cells to establish their phenotype and determine some of their functions. by means of immunocytochemistry and cytofl ...19921347309
synthesis, cardiac electrophysiology, and beta-blocking activity of novel arylpiperazines with potential as class ii/iii antiarrhythmic agents.a series of novel arylpiperazines have been prepared in an attempt to incorporate both class ii (beta-receptor blocking) and class iii antiarrhythmic properties in a single molecule. the key step in the preparation of the new compounds involves a regioselective heterocyclic ring formation. all but four compounds significantly prolonged action potential duration in canine cardiac purkinje fibers (class iii activity). all but one of the compounds demonstrated beta-receptor affinity in a competitiv ...19921347318
isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting.the carotenoid pigment astaxanthin (3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) is an important component in feeds of aquacultural animals. it is produced as a secondary metabolite by the yeast phaffia rhodozyma, and the isolation of rare mutants that produce increased quantities is limited by the lack of genetic selections. as a model system for enriching mutants increased in production of secondary metabolites, we have used quantitative flow cytometry/cell sorting (fccs) to isolate astaxanth ...19911367215
activation of t cells in vivo using anti-cd3 and staphylococcal enterotoxins.one of the major problems in clinical immunity is that neoplastic and virally infected cells are insufficiently immunogenic to trigger an immune response. during the past several years, our laboratory has explored the use of t-cell-specific reagents including monoclonal antibodies (mabs) and staphylococcal enterotoxins to amplify immune responses. this report summarizes our efforts to augment immunity with these agents.19921428401
ventilator-assisted patient vocalization with positive end-expiratory pressure and tracheostomy cuff leak: a brief report.to enable vocalization in a tracheostomized ventilator-assisted patient without compromising pulmonary status.19921447005
analysis of polar lipids from some representative enterobacteria, plesiomonas and acinetobacter by fast atom bombardment-mass spectrometry.fast atom bombardment-mass spectrometry (fab-ms) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. the following micro-organisms were tested: acinetobacter calcoaceticus, acinetobacter sp., citrobacter freundii, enterobacter cloacae (2 strains), escherichia coli (3 strains), hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, morganella morganii, plesiomonas shigelloides, proteus mirabilis (3 strain ...19921447059
evaluation of the autoscan-w/a rapid system for identification and susceptibility testing of gram-negative fermentative bacilli.the autoscan-walk-away (w/a) system for identification and susceptibility testing was evaluated for 400 gram-negative fermentative bacteria by using the api 20e (366 isolates) and/or tube biochemical tests as the reference identification system and a frozen microdilution mic tray system for susceptibility testing. the w/a system performed well for identification of this group of organisms representing 14 genera and 30 species, showing a sensitivity of 96% and results available in 2 h. of the 16 ...19921452661
[kinetics of bactericidal and sporicidal effects of a disinfectant against bacteria isolated from hospital units].the bactericidal and sporicidal activities of a peracetic acid disinfectant commonly used in hospitals (acetoper 200) was studied using three bacterial species recovered in water from hemodialysis machines and humidifiers, i.e., pseudomonas aeruginosa, serratia liquefaciens, and bacillus subtilis (spores). the method used was derived from afnor norms nf t 72-151 and nf t 72-231. for each strain, three concentrations of disinfectant were tested. counts were performed every five minutes for one ho ...19921495838
iron transport systems of serratia marcescens.serratia marcescens w225 expresses an unconventional iron(iii) transport system. uptake of fe3+ occurs in the absence of an iron(iii)-solubilizing siderophore, of an outer membrane receptor protein, and of the tonb and exbbd proteins involved in outer membrane transport. the three sfuabc proteins found to catalyze iron(iii) transport exhibit the typical features of periplasmic binding-protein-dependent systems for transport across the cytoplasmic membrane. in support of these conclusions, the pe ...19921531225
the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes.chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ...19921594593
neuromuscular complications of human immunodeficiency virus infection.as the lifespan of patients with hiv infection is prolonged with more effective antiviral therapies, the prevalence of neuromuscular disorders is likely to increase. the understanding of the pathogenesis and optimal therapy of these illnesses has advanced during the past 5 years. however, there are still significant gaps in our knowledge. it is important that neurologists remain at the forefront of investigation and treatment of these complex disorders.19921615238
eosinophil chemotactic peptide sequences in rat alpha-cgrp. activation of a novel trophic action by neutral endopeptidase 24.11.rat alpha- and alpha-cgrp are substrates for endopeptidase 24.11 in vitro. cleavage of both peptides occurs at several points, including an unusual substrate recognition site to the amino side of ala36. in alpha-cgrp this resulted in the early formation of val32-gly-ser-glu35, a sequence previously reported to be a component of the eosinophil chemotactic factor of anaphylaxis (ecf-a). the biological activity of this peptide fragment was confirmed by bioassay. chemotactic activity in other hydrol ...19921637096
expression of extracellular phospholipase from serratia liquefaciens is growth-phase-dependent, catabolite-repressed and regulated by anaerobiosis.many members of the genus serratia synthesize and excrete a number of extracellular hydrolytic enzymes. one of these is the phospholipase a1 from serratia liquefaciens, the expression of which is growth-phase-dependent. through the use of gene fusions and primer extension analysis we show that the expression of phospholipase is subject to positive transcriptional regulation of a dual promoter system; one promoter positioned approximately 600bp upstream from the phla gene is responsible for the i ...19921640837
nicotine, acetylcholine and bombesin are trophic growth factors in neuroendocrine cell lines derived from experimental hamster lung tumors.neuroendocrine hamster lung tumors, induced by exposure to 60% hyperoxia and subcutaneous administration of the tobacco-specific nitrosamine 4-(methylnitrosamino)-l-(3-pyridyl)-l-butanone (nnk) for 12 weeks, were placed in cell culture. by subsequent selective transfer of epithelial cells and maintenance in an atmosphere of 8% co2, cell lines with characteristics of neuroendocrine cells were established. the neuroendocrine markers expressed by these cell lines included electron dense neuroendocr ...19901698239
monoclonal antibodies to quebec strain (q17) of bovine rotavirus.fourteen monoclonal antibodies (mabs) against "quebec" strain (q17) of bovine rotavirus were isolated and characterized. four were specific for viral protein vp7 and ten were specific for viral protein vp6. five different isotypes were represented by this group of antibodies. all of the anti-vp6 and none of the anti-vp7 antibodies were sensitive to the effects of periodate on their antigen. the antibodies could be separated into three groups based on their relative resistance to the dissociation ...19901698323
desensitization of the dopaminergic system in bovine retina following incubation with high potassium.the effect of potassium depolarization on dopamine d1 receptor activity in bovine retina was investigated. preincubation of bovine retinas in buffer containing high kcl (56 mm) as compared to a low kcl control buffer resulted in a significant decrease in dopamine-stimulated adenylate cyclase activity with no change in basal or gtp-stimulated adenylate cyclase activity. the apparent vmax for dopamine was decreased from 102 +/- 15 pmol/min/mg protein in retinas preincubated in high kcl to 71 +/- 1 ...19901698510
[diagnosis and therapy of viral hepatitis]. 19911713243
comparative biotyping, bacteriocin typing, and serogrouping (o-antigens) of serratia liquefaciens.a total of 83 clinical isolates of serratia liquefaciens from 81 patients were biotyped, bacteriocin typed (with group a phage tail bacteriocins from s. marcescens), and serogrouped. biotyping afforded least discrimination, because 71 of the 83 isolates (85.5%) comprised 2 biotypes; the remainder were of 5 different biotypes. bacteriocin typing differentiated 70 of the 83 isolates (84.3%) into 20 types. polyclonal rabbit anti-o immune sera identified 16 o-antigens, and all 83 isolates could be s ...19911718303
serotyping of serratia liquefaciens: h-antigens.polyclonal anti-h rabbit immune sera differentiated 12 flagellar (h) antigens among 79 motile of 83 clinical isolates of serratia liquefaciens from 81 patients. seven of the anti-h s. liquefaciens sera cross-reacted with h-antigens of s. marcescens, specifically anti-s. liquefaciens h6, h7, h8, h9, h10, h11, and h12 with s. marcescens h-antigens h7, h8, h12, h14, h5, h3, and h17, respectively. these cross-reactions were reciprocal.19911718304
metalloproteases of serratia liquefaciens: degradation of purified human serum proteins.two representative strains of serratia liquefaciens, sl 5 (serotype o5:h1) and sl 11 (serotype o1:h1), produced proteases characterized by molecular weights of 52.5 kilodaltons and isoelectric points of 6.2; both enzymes were inhibited by 50 mm edta. as demonstrated with sds-page electrophoresis, the two metalloproteases attacked the following purified human serum proteins: complement components c3, c4, c5, c6, c7, c8, and c9, transferrin, alpha 1-antitrypsin, alpha 2-macroglobulin, fibronectin, ...19911724153
combination of long and short term gnrh analogue protocols: a new therapeutic approach to persistent high progesterone levels in ivf cycles.thirty-four patients who had had persistently high progesterone (p) levels during menotrophin therapy in previous in-vitro fertilization (ivf) cycles, despite pretreatment with a long acting gonadotrophin-releasing hormone analogue (gnrha), were randomly divided into two groups according to the mode of ovulation induction used. in 16 women (group 1), induction of ovulation was performed with decapeptyl (dtrp6)/pure follicle-stimulating hormone (pfsh)/human menopausal gonadotrophin (hmg)/human ch ...19911829091
influence of dietary alpha-tocopherol on tocopherol concentrations in chick tissues.1. the effect of feeding alpha-tocopherol (5 to 180 micrograms/g diet) for 24 days on the concentrations of alpha-tocopherol in various chicken tissues was investigated. 2. tissue alpha-tocopherol concentrations responded to dietary intake in the order: heart congruent to lung greater than liver greater than thigh muscle greater than brain, and in all cases the relationship between the concentrations of dietary and tissue alpha-tocopherol was highly significant (0.997 less than or equal to r les ...19911868377
intravenous ciprofloxacin for the treatment of severe infections.intravenous ciprofloxacin at a daily dosage of 400 mg divided in two doses was administered to 19 patients with severe infections caused by ciprofloxacin-susceptible bacteria. these infections included: 11 surgical would infections, 5 soft tissue infections, 2 respiratory tract infections, 1 urinary tract infection. the offending pathogens were: 8 coagulase-negative staphylococci, 3 staphylococcus aureus, 3 pseudomonas aeruginosa, 2 proteus spp., 1 escherichia coli, 1 branhamella catarrhalis, 1 ...19911875231
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