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construction of an aroa mutant of salmonella serotype gallinarum: its effectiveness in immunization against experimental fowl typhoid.an aroa mutant was produced from a virulent strain of salmonella gallinarum, the causative agent of fowl typhoid in poultry, by tn10 insertional inactivation and deletion. the mutant was highly attenuated for chickens. a single intramuscular immunization of 2-week-old chickens with 10(7) mutant organisms reduced mortality following oral challenge with 10(8) organisms of the parent strain from 63 to 30%. in a second experiment multiple immunizations with 10(8) mutant organisms reduced mortality a ...19938470431
serological monitoring of 40 swiss fancy breed poultry flocks.rapid serum agglutination, haemagglutination inhibition and enzyme-linked immunosorbent assays were used to screen swiss fancy breed chicken flocks for antibodies against 12 avian infectious agents. for this purpose, 1,002 blood samples from 40 flocks were collected and tested. ten percent of the samples were positive for salmonella gallinarum-pullorum and 62.5% of the flocks were affected. more than 75% of the flocks had antibodies against mycoplasma gallisepticum/mycoplasma synoviae, infectiou ...200212396360
[pharmacological, toxicological, microbiological and pathohistological studies of gentamycin complex ii].the acute toxicity was determined of the gentamycin complex ii (gmc-ii) for albino mice, containing gentamycin components in a varying ratio. the ld50 at intraperitoneal application was 347.7 mg/kg body mass, while at oral application it was 5,180 mg (4933 divided by 5439) mg/kg body mass. histopathologically, (mice killed with high doses of gmc-ii, given orally) there were diffuse hyperemia of the liver, parenchymal degeneration of the kidney tubuli epithelium, and edema and emphysema of the lu ...19836623911
reduction in incidence of experimental fowl typhoid by incorporation of a commercial formic acid preparation (bio-add) into poultry feed.the experiment described evaluated the effect of a commercial in-feed preparation (bio-add) involving a mixture of formic acid and propionic acid on the incidence of experimental fowl typhoid in groups of 41 and 42 1-wk-old rhode island red chickens. the chickens were infected through contact with 12 identical chickens that had been inoculated orally with 10(8) cfu of salmonella gallinarum strain 9. the incidence of mortality and morbidity due to fowl typhoid was 31/41 (76%) in birds given untre ...19968778726
immunoprophylaxis of salmonella gallinarum infection by salmonella enteritidis-immune lymphokines in broiler chicks.research on the control of intestinal and tissue colonization of breeder and table-egg producing flocks by invasive salmonella enteritidis (se) has focused on the advancement of anti-salmonella feed additives, microbiological strategies, and the development of vaccines. recent investigations in our laboratories have concentrated on the development of immunoprophylactic measures to control salmonella infections. we have found an increased resistance to salmonella enteritidis (se) organ infectivit ...19979192048
food as a vehicle of infection; the effect of variations in the diet on the induction of salmonella gallinarum infection. 195413208934
lead disrupts eicosanoid metabolism, macrophage function, and disease resistance in birds.lead (pb) affects elements of humoral and cell-mediated immunity, and diminishes host resistance to infectious disease. evidence is presented supporting a hypothesis of pb-induced immunosuppression stemming from altered fatty acid metabolism, and mediated by eicosanoids and macrophages (mo). chronic pb exposure increases the proportion of arachidonate (ara) among fatty acids in lipid from avian tissues, and this change provides precursors for eicosanoids, the oxygenated derivatives of ara that m ...19979404672
[salmonella gallinarum-pullorum infection of poultry: experiences in switzerland].37 salmonella gallinarum-pullorum-strains were isolated from dead poultry between 1986 and 1996. all strains except one belonged to the biovar pullorum. 33 isolates were from fancy poultry flocks, four from backyard flocks. 10 of 12 chicks and 10 of 25 adult birds showed clinical signs of disease. the growing significance of fancy and backyard flocks as a reservoir for s. gallinarum-pullorum is emphasized in view of the increase in free range management of commercial layer and broiler flocks.19979480540
antigenic studies using ammonium sulfate. iv. the sedimentation effect of ammonium sulfate on salmonella gallinarum. 195613340122
variants of smooth salmonella enterica serovar enteritidis that grow to higher cell density than the wild type are more virulent.salmonella enterica serovar enteritidis that grows to a higher cell density (se-hcd) than wild type while retaining o-chain lipopolysaccharide was isolated by transforming wild type serovar enteritidis with the cell density sensor plasmid psb402 and selecting for bioluminescence. a luminescent strain, se-hcd, that emitted light in proportion with cell density and opacity through stationary phase was isolated. after a peak cell density of 1.5 x 10(11) cfu/ml was observed, luminescence decreased, ...19989603830
[immunochemical study of salmonella. ii. analysis of precipitins of some anti-salmonella gallinarum and anti-salmonella typhi sera]. 195613363029
protection of chickens against experimental fowl typhoid using a nuog mutant of salmonella serotype gallinarum.a nuog mutation in nadh dehydrogenase i was introduced into a virulent strain of salmonella serotype gallinarum, the causative agent of fowl typhoid, using gene replacement with a nuog open reading frame inactivated by insertion of dna encoding a kanamycin resistance determinant. the s. gallinarum nuog mutant, named sg9ngk, was highly attenuated in chickens. sg9ngk colonized the caeca of chickens less efficiently than the s. gallinarum parental strain, was less invasive and showed no evidence of ...19989682335
effect of a commercial competitive exclusion culture (preempt) on mortality and horizontal transmission of salmonella gallinarum in broiler chickens.a commercial competitive exclusion (ce) culture (preempt) was evaluated for its ability to decrease mortality during the first 10-12 days posthatch resulting from the causative agent of fowl typhoid, salmonella gallinarum. in the first experiment, chicks provided the ce culture on day of hatch and challenged with 10(5) s. gallinarum on day 3 had a significant decrease in mortality compared with non-ce-treated s. gallinarum-challenged chicks in all four replicates. mortality for control chicks wh ...19989876831
antigenic analysis of d-valine adapted salmonella gallinarum. 195613376505
salmonella pullorum in the common pheasant (phasianus colchicus).in 1996, pullorum disease due to salmonella enterica serovar gallinarum biovar pullorum (salmonella pullorum) was diagnosed in pheasants on a gamebird rearing enterprise in south-west scotland. the gross pathology and bacteriological findings are described, as are the results of screening for s pullorum on the site in 1997. the causal organism was readily isolated from the lung, liver, yolk sac and heart blood on direct culture, but less readily from the digestive tract or by the use of selectiv ...199910204223
attempt to stimulate lymphatic gland changes of fowl typhoid with adrenal cortex extract. 195713465781
[investigation on antigenic value of isolated in bulgaria strains of salmonella gallinarum (syn. salmonella pullorum) in diagnosis of pullorosis before incubation]. 195813549099
pathological changes in the blood of chickens infected with salmonella gallinarum. 196013806562
the in vivo sensitization of avian erythrocytes with salmonella gallinarum polysaccharide. 195913806563
[on the nutrition of salmonella pullorum and salmonella gallinarum. 3. on the purine requirement of salmonella pullorum strain no. 21]. 196213938795
vaccination for control of salmonella in poultry.salmonella spp. are facultative intracellular pathogens causing localised or systemic infections, in addition to a chronic asymptomatic carrier state. they are of worldwide economic and public health significance. in poultry, which represent important sources of cheap protein throughout the world, fowl typhoid and pullorum disease continue to cause economic losses in those parts of the world where the poultry industries are continuing to intensify and where open sided housing is common. a number ...199910418900
studies on immunity and pathogenesis of salmonellosis. i. antigen-antibody reactions on circulating leucocytes of chickens infected with salmonella gallinarum. 196314088890
studies on immunity and pathogenesis of salmonellosis. ii. antibody production and accumulation of bacterial polysaccharide in the tissues of chickens infected with salmonella gallinarum. 196314088891
phosphine generation by mixed- and monoseptic-cultures of anaerobic bacteria.a microbial basis for bioreductive generation of phosphine is proposed, which could account at least in part for the presence of this toxic gas in natural anaerobic environments and in sewage and landfill gases. phosphine generation under anaerobic growth conditions was dependent upon both the culture inoculum source (animal faeces) and enrichment culture conditions. phosphine was detected in headspace gases from mixed cultures under conditions promoting fermentative growth of mixed acid and but ...200010811253
fowl typhoid and pullorum disease.fowl typhoid (ft) and pullorum disease (pd) are septicaemic diseases, primarily of chickens and turkeys, caused by gram negative bacteria, salmonella gallinarum and s. pullorum, respectively. clinical signs in chicks and poults include anorexia, diarrhoea, dehydration, weakness and high mortality. in mature fowl, ft and pd are manifested by decreased egg production, fertility, hatchability and anorexia, and increased mortality. gross and microscopic lesions due to ft and pd in chicks and poults ...200010935271
attempts to purify the salmonella gallinarum complex o-antigen by fractionation over ion-exchange deae-cellulose and gel-filtration. 196414182620
effect of salmonella gallinarum on certain krebs cycle intermediates of domestic fowl.freeman, b. m. (houghton poultry research station, huntingdon, england), and l. g. chubb. effect of salmonella gallinarum on certain krebs cycle intermediates of domestic fowl. j. bacteriol. 88:93-95. 1964.-in chickens infected with salmonella gallinarum, the citric acid content of the blood rose but that of the liver fell significantly. the former may be due solely to anorexia; the latter is suggested to be a result of the inhibition of the reaction between pyruvic and oxaloacetic acids. pyruvi ...196414197910
differential cytokine expression in avian cells in response to invasion by salmonella typhimurium, salmonella enteritidis and salmonella gallinarum.salmonella enterica is a facultative intracellular pathogen that is capable of causing disease in a range of hosts. although human salmonellosis is frequently associated with consumption of contaminated poultry and eggs, and the serotypes salmonella gallinarum and salmonella pullorum are important world-wide pathogens of poultry, little is understood of the mechanisms of pathogenesis of salmonella in the chicken. type iii secretion systems play a key role in host cell invasiveness and trigger th ...200011101679
[studies on toxic properties and composition of complex salmonella gallinarum antigens obtained from bacterial suspension treated and untreated with ultrasonics]. 196414266482
[comparative study of water-soluble proteins of initial and phageresistant variants of salmonella gallinarum-pullorum by paper electrophoresis]. 196414301106
[lysogenic conversion of the 01 antigen of the salmonella gallinarum-pullorum serotype]. 196414305626
bacterial colonization and endotoxin activity during experimental acute fowl typhoid in chickens.bacterial colonization and endotoxin production were investigated before and after experimental salmonella gallinarum infection in 8-week-old female broiler chickens. these parameters were assayed by means of colony forming units test (cfu) and the limulus amebocyte lysate test (lal), respectively. birds were infected per os with 1,5 x 10(9) cfu/ml of wild strain of s. gallinarum isolated from a dead hen. approximately 1,5 x 10(2); 1,3 x 10(2) and 1,2 x 10(2) cfu of s. gallinarum were recorded f ...200011206397
studies of the phenomenon of host adaptation in salmonella.to study the phenomenon of host adaptation in salmonella, a mathematical model has been developed which permits a definition and experimental investigation of the specific interaction between the adapted serovar and the adequate host. after experimental infection using a mixture of equal parts of two salmonella strains, a and b, the bacterial concentrations ca and cb were determined in the organs of the animals infected. if an animal of species a and an animal of species b are infected with the ...200011244872
comparative activity of nihydrazone and furazolidone against salmonella gallinarum and escherichia coli in chickens. 196514340751
vaccination against salmonella enteritidis in dutch commercial layer flocks with a vaccine based on a live salmonella gallinarum 9r strain: evaluation of efficacy, safety, and performance of serologic salmonella tests.this study describes a field trial in which 80 commercial layer flocks, with an increased risk of salmonella enteritidis (se) infection and placed on farms with a certified standardized biosecurity programme (sbp) or a request for a sbp certificate, were vaccinated with a vaccine based on a live attenuated salmonella gallinarum (sg) 9r strain. an evaluation is presented of the efficacy of the vaccine against se infections, the effect on the performance of serologic salmonella tests, and the spre ...200111332503
salmonella enterica serovar gallinarum requires the salmonella pathogenicity island 2 type iii secretion system but not the salmonella pathogenicity island 1 type iii secretion system for virulence in chickens.salmonella enterica serovar gallinarum is a host-specific serotype that causes the severe systemic disease fowl typhoid in domestic poultry and a narrow range of other avian species but rarely causes disease in mammalian hosts. specificity of the disease is primarily at the level of the reticuloendothelial system, but few virulence factors have been described other than the requirement for an 85-kb virulence plasmid. in this work, by making functional mutations in the type iii secretion systems ...200111500419
[transduction of a somatic antigen during infection of salmonella gallinarum with a temperate phage of salmonella typhimurium]. 195914412466
evaluation of a new chromogenic medium for the isolation and presumptive identification of salmonella species from stool specimens.the performance of bbl chromagar salmonella (becton dickinson, france), a new selective chromogenic medium for the isolation and presumptive identification of salmonella spp., was evaluated. on this medium, which is a modification of chromagar salmonella (chromagar microbiology, france) with enhanced selectivity, the colonies of salmonella are stained in mauve (rose-violet), while those of other organisms appear in blue-green or are not stained by any of the chromogens of the medium. the medium ...200111681435
classification of grossly detectable abnormalities and conditions seen at postmortem in canadian poultry abattoirs according to a hazard identification decision tree.this study was designed to review all grossly detectable abnormalities and conditions (gdacs) encountered in poultry in canadian abattoirs to determine which have potential to cause adverse health effects for the consumer. review of the literature and consultation with scientists in the field of microbiology, epidemiology, poultry pathology, chemistry, and meat inspection served to generate an inventory of gdacs, and a decision tree containing algorithms was developed to identify gdacs potential ...200111770626
adhesion of probiotic lactobacilli to chick intestinal mucus.in the present work, interactions between three lactobacillus strains (lactobacillus fermentum crl1015, lactobacillus animalis crl1014, and lactobacillus fermentum crl1016) and chicken small intestinal mucus were determined. three lactobacilli isolated from chicken and selected by their potentially probiotic properties were able to grow in mucus preparations. three peaks from gel filtration chromatography of intestinal mucus were obtained. the adhesion to three mucus fractions (i, ii, and iii), ...200314569288
the spread of salmonella gallinarum 9r vaccine strain under field conditions.a live vaccine based on an attenuated salmonella gallinarum 9r strain is in use in a salmonella enteritidis control program in commercial layer flocks in the netherlands. in a field study, the potential spread of the vaccine strain from vaccinated flocks to nonvaccinated flocks has been studied after both the primary and the booster injection at four different rearing farms and at one layer farm. the vaccinated and the nonvaccinated flocks were monitored at regular intervals by bacteriologic and ...200111785873
competitive exclusion against salmonella gallinarum of salmonella enteritidis infected chickens.to evaluate the degree of competitive exclusion against salmonella gallinarum(s. gallinarum) of salmonella enteritidis(s. enteritidis) infected chickens, fifty-six, 4-week old hyline layer suspected of s. enteritidis infection were challenged with s. gallinarum. all chickens were tested for s. enteritidis isolation using cloacal swabs and serum plate agglutination test using s. enteritidis ag. before challenge and classified into four groups(se isolated, se nonisolated, se seropositive and se se ...200114614291
fowl typhoid. i. comparison of antigenicity of sixteen gallinarum antigens. 195114801734
the effect of aureomycin against salmonella gallinarum in vivo and in vitro. 195114829770
the effect of sulfonamides on the control of fowl typhoid in chickens. 195214927495
efficacy of crude extract of aloe secundiflora against salmonella gallinarum in experimentally infected free-range chickens in tanzania.the ethnoveterinary use of aloe species extract in free-range local chickens against fowl typhoid was investigated. five-months-old local chickens, free of antibodies against fowl typhoid were used. the chickens were randomly assorted into five groups including pretreated and infected (g1, n=21), infected and untreated (g2, n=21), infected and treated (g3, n=21), untreated and uninfected (g4, n=10) and treated uninfected (g5, n=10). groups 1, 2 and 3 were inoculated with 5.0 x 10(8) c.f.u/ml of ...200211849835
nucleotide and amino acid sequences of orit-tram-traj-tray-traa-tral regions and mobilization of virulence plasmids of salmonella enterica serovars enteritidis, gallinarum-pullorum, and typhimurium.the virulence plasmid of salmonella enterica serovar gallinarum-pullorum (pspv) but not those of salmonella enterica serovars enteritidis (psev) and typhimurium (pstv) can be readily mobilized by an f or f-like conjugative plasmid. to investigate the reason for the difference, the orit-tram-traj-tray-traa-tral regions of the three salmonella virulence plasmids (psvs) were cloned and their nucleotide and deduced amino acid sequences were examined. the cloned fragments were generally mobilized mor ...200212003924
allele-specific pcr method based on rfbs sequence for distinguishing salmonella gallinarum from salmonella pullorum: serotype-specific rfbs sequence polymorphism.cloning and sequence analysis of rfbs gene identified two polymorphic nucleotides, one at position 598 (salmonella gallinarum-specific) and other at position 237 (salmonella pullorum-specific). based on s. gallinarum-specific nucleotide found at position 598, an allele-specific pcr method was developed for serotype-specific detection of s. gallinarum. this pcr method was able to discriminate pure cultures of s. gallinarum from s. pullorum and other salmonella serotypes from serogroup d in less t ...200515590091
comparative pathogenesis of experimental infections with salmonella gallinarum in local and commercial chickens.the pathogenicity of a virulent strain of salmonella gallinarum was studied in local chickens and commercial layers. sixty 4-month-old chickens were used. of these, 50 chickens were orally infected with s. gallinarum, comprising 25 commercial layers and 25 local chickens. five chickens in each group were used as uninfected negative controls. the clinical signs and pathological features of acute, subacute and chronic fowl typhoid were observed in both groups. chickens in both groups seroconverted ...200212094675
distribution and characterization of class 1 integrons in salmonella enterica serotype gallinarum biotype gallinarum.fowl typhoid caused by salmonella enterica subsp. enterica serotype gallinarum biotype gallinarum is the most important chicken disease in korea. due to appearance of new or multiple antibiotics resistances in the recently isolated strains, it was difficult to control the disease using antibiotics in our country. therefore, the prevalence and genetic contents of class 1 integrons in biotype gallinarum isolated between 1992 and 2001 were investigated by pcr and direct sequencing, respectively. ou ...200212383639
the genome of salmonella enterica serovar gallinarum: distinct insertions/deletions and rare rearrangements.salmonella enterica serovar gallinarum is a fowl-adapted pathogen, causing typhoid fever in chickens. it has the same antigenic formula (1,9,12:--:--) as s. enterica serovar pullorum, which is also adapted to fowl but causes pullorum disease (diarrhea). the close relatedness but distinct pathogeneses make this pair of fowl pathogens good models for studies of bacterial genomic evolution and the way these organisms acquired pathogenicity. to locate and characterize the genomic differences between ...200515995186
comparison of the immune responses against salmonella enterica serovar gallinarum infection between naked neck chickens and a commercial chicken line.the immune responses of indigenous naked neck (nana and nana) and normally feathered (nana) chickens against a salmonella gallinarum (sg) infection were evaluated and compared with those of a commercial line (b-380). groups of 28-day-old chickens (nana, nana, nana, and b-380) were immunized orally and subcutaneously with 50 microg of sg antigens. control non-immunized animals were inoculated with sterile saline solution. all chickens were challenged with 1 ld(50) of sg and mortality was recorded ...200312745372
the isolation of salmonella gallinarum in wild birds. 195513242673
the longevity of salmonella gallinarum in the faeces of infected chickens. 195513242676
differentiation of salmonella gallinarum biovar gallinarum from salmonella gallinarum biovar pullorum by pcr-rflp of the fimh gene.in our studies on fimh adhesins expressed by different salmonella serovars, we cloned and sequenced the fimh genes from salmonella enterica ssp. enterica ser. gallinarum biovar gallinarum and s. enterica ssp. enterica ser. gallinarum biovar pullorum. comparison of the nucleotide sequences revealed the presence of a single-nucleotide polymorphism (snp) at position 544 bp from the a of the start codon of the fimh open reading frame (orf). further analysis of the restriction enzyme sites in fimh ge ...200516115094
[invalidness of the distinction between salmonella gallinarum and salmonella pullorum and their mutual biochemical variants]. 195613340328
the use of live vaccines in experimental salmonella gallinarum infection in chickens with observations on their interference effect. 195613367409
safety and efficacy of salmonella gallinarum 9r vaccine in young laying chickens.salmonella enterica subsp. enterica serovar gallinarum (s. gallinarum) is the agent of fowl typhoid, and the 9r vaccine is a commercially available, live vaccine for the prevention of fowl typhoid. the aim of this study was to assess the safety and efficacy of the 9r vaccine in young chickens. the mean weights of 5-week-old chickens vaccinated with one and 10 doses at 2 weeks old were 450.3+/-33.83 g and 446.8+/-35.68 g, respectively, which were statistically lower (p<0.05) than the mean weight ...200516147574
the immunity to salmonella gallinarum infection in chickens produced by live cultures of members of the salmonella genus. 195613367410
the effect of feeding different levels of protein concentrates on the susceptibility of chickens to salmonella gallinarum infection. 195713406119
the differentiation of salmonella pullorum and salmonella gallinarum by biochemical methods. 196213993927
fructose-6-phosphate reductase from salmonella gallinarum.zancan, glaci t. (universidade do paraná, curitiba, paraná, brazil), and metry bacila. fructose-6-phosphate reductase from salmonella gallinarum. j. bacteriol. 87:614-618. 1964.-a fructose-6-phosphate reductase present in cell-free extracts of salmonella gallinarum was purified approximately 42 times. the optimal ph for this enzyme is 8.0. the enzyme is specific for fructose-6-phosphate and reduced nicotinamide adenine dinucleotide (nadh). the dissociation constants are 1.78 x 10(-4)m for fructo ...196414127579
[a case of salmonella gallinarum-pullorum infection]. 196414138791
[purification of complex salmonella gallinarum o-antigen by the fractionation on ion-exchange deae-cellulose and gel filtration]. 196414266475
[studies on the antigenic structure and serologic behavior of salmonella gallinarum. 1. studies on the distribution of individual antigens in various salmonella gallinarum strains]. 196514314882
biochemical differentiation of salmonella gallinarum and salmonella pullorum cultures. 196514334406
cultural and antigenic studies on salmonella gallinarum and salmonella pullorum. 192716559234
the reduction of nitrates to nitrites by salmonella pullorum and salmonella gallinarum. 193016559426
a comparative study of the nutritional requirements of salmonella typhosa, salmonella pullorum and salmonella gallinarum. 194316560616
cytotoxic and antimicrobial benzophenones from the leaves of tovomita longifolia.bioassay-guided fractionation of the chloroform and ethanol extracts of tovomita longifolia leaves using cytotoxic and antimicrobial assays resulted in the isolation of four new benzophenones, (e)-3-(2-hydroxy-7-methyl-3-methyleneoct-6-enyl)-2,4,6-trihydroxybenzophenone (1), (e)-3-(6-hydroxy-3,7-dimethylocta-2,7-dienyl)-2,4,6-trihydroxybenzophenone (2), 8-benzoyl-2-(4-methylpenten-3-yl)chromane-3,5,7-triol (3), and 5-benzoyl-1,1,4a-trimethyl-2,3,4,4a,9,9a-hexahydro-1h-xanthene-6,8-diol (4), and ...200616562847
[variations in carbohydrate metabolism by salmonella gallinarum and salmonella pullorum; their diagnostic value]. 195414350347
influence of high levels of vitamins on resistance of chicks to fowl typhoid. 195514371690
water-soluble nitrofuran therapy in pullorum and fowl typhoid in chicks. 196214491993
occurrence and regulation of the multicellular morphotype in salmonella serovars important in human disease.multicellular behavior in salmonella typhimurium atcc14028 called the rdar morphotype is characterized by the expression of the extracellular matrix components cellulose and curli fimbriae. over 90% of s. typhimurium and s. enteritidis strains from human disease, food and animals expressed the rdar morphotype at 28 degrees c. regulation of the rdar morphotype occurred via the response regulator ompr, which activated transcription of csgd required for production of cellulose and curli fimbriae. s ...200314503792
biochemical characteristics and antimicrobials susceptibility of salmonella gallinarum isolated in korea.fowl typhoid (ft) reported since 1992 in korea is a septicemic disease of domestic birds caused by salmonella gallinarum (s. gallinarum). the purpose of this study was to investigate the biochemical characteristics and antimicrobials susceptibility of field isolates of s. gallinarum isolated by year in korea. a total of 258 isolates of s. gallinarum from 1995 to 2001 showed the same pattern in the majority of biochemical test such as imvic (indole, methyl red, voges-proskauer and citrate utiliza ...200314610370
typing of salmonella gallinarum and salmonella pullorum by means of bacteriophage. 195214933053
fowl typhoid in a small backyard laying flock. 200717351184
salmonella gallinarum gyra mutations associated with fluoroquinolone resistance.salmonella enterica subsp. enterica serovar gallinarum (s. gallinarum) is the causative organism of fowl typhoid, and an outbreak of fowl typhoid in korea was confirmed in 1992. the aim of this study was to investigate possible changes in fluoroquinolone susceptibility among s. gallinarum isolates from 1995 to 2001, and to analyse mutations of the gyra gene in fluoroquinolone-resistant isolates. among 258 s. gallinarum isolates tested by the disk diffusion method, isolates from 1995 (n=18) were ...200415276996
characterization of a broad range antibacterial substance from a new bacillus species isolated from amazon basin.a bacillus sp. strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16s rdna sequencing. the phylogenetic analysis indicated that this strain has low sequence similarity with most bacillus spp., suggesting a new species was isolated. the antimicrobial activity was detected starting at the exponential growth phase, and maximum activity was observed at stationary phase. the substance was inhibitory to a broad range of indicator strains, including pathogenic ...200717534601
influence of antibiotics on growth dynamics and movement ability of salmonella rods.variety of traits important in diagnostics and epidemiology of pathogenic microorganisms may change due to antibiotics. movement ability, that is characteristic for every serovar except from salmonella gallinarum-pullorum, is important to salmonellas. in own experiments using semi-fluid msrv medium, it was found that a decrease in salmonella sensibility to selected antibiotics and chemiotherapeutics due to passage might lead to weakening of its movement ability. movement ability of all strains ( ...200415633786
development of a pcr system for the characterisation of salmonella flagellin genes.analysis of flagellin genes was carried out on strains of salmonella typhimurium, salmonella hadar, salmonella abortusequi, salmonella enteritidis and salmonella gallinarum serovars, using a pcr system designed in this study. the purpose of these studies was to explore the flagellin genes of biphasic and monophasic salmonellae for future targeted genetic interventions. the pcr primers were designed for two different structural genes of flagellin (flic, fljb), for the repressor of flic (flja), fo ...200515959975
control of salmonella gallinarum in swiss fancy breed flocks. 200717720967
differential diagnosis of salmonella gallinarum and s. pullorum using pcr-relp.salmonellosis in poultry of korea is a significant health problem, which causes substantial economic losses. the most common causative agents of chicken salmonellosis ar s. gallinarum and s. pullorum. traditional methods used to detect salmoenella spp. in chicken are tedious, time consuming and confer little guarantee of sensitivity and species specificity. therefore, a rapid and sensitive method for the differentiation of salmonella serogroup d was assessed. we first amplified the rfbs genes by ...200112441691
comparison of intestinal invasion and macrophage response of salmonella gallinarum and other host-adapted salmonella enterica serovars in the avian host.the purpose of this investigation was to study the host specific infection of salmonella gallinarum in chickens and to determine the contribution of intestinal invasion and macrophage survival in relation to systemic infection in the host. this was carried out by comparing the kinetics of infection of s. gallinarum to that of other salmonella host-adapted (s. cholerae-suis, s. dublin and s. typhimurium) and host-specific (s. pullorum and s. abortus-ovis) serovars. establishment of the rate of co ...200312488070
effects of fusarium moniliforme culture material containing known levels of fumonisin b1 on progress of salmonella gallinarum infection in japanese quail: clinical signs and hematologic studies.to study the individual and combined effects of fumonisin b1 (fb1) toxicity and salmonella serotype gallinarum infection, japanese quail (coturnix coturnix japonica) were fed fusarium moniliforme culture material (2.5%), 150 mg fb1/kg ration, and were subsequently challenged orally with salmonella gallinarum organisms (2 x 10(4) colony-forming units) at 21 days of age. the chicks were fed culture material containing fb1 from day 5 till the end of the experiment. after being infected with salmone ...200516094834
characterization of fimh adhesins expressed by salmonella enterica serovar gallinarum biovars gallinarum and pullorum: reconstitution of mannose-binding properties by single amino acid substitution.recombinant fimh adhesins of type 1 fimbriae from salmonella enterica serovar gallinarum biovars gallinarum and pullorum, in contrast to those of salmonella enterica serovar typhimurium, did not bind to high-mannose oligosaccharides or to human colon carcinoma ht-29 cells. however, mutated fimh proteins from biovar gallinarum and biovar pullorum, in which the isoleucine at position 78 was replaced by the threonine found in s. enterica serovar typhimurium, bound well to glycoproteins carrying hig ...200516113346
fowl typhoid in caged layer birds. 200516127143
comparison of a dot immunobinding assay and the serum agglutination test for detecting serological responses in vaccinated and unvaccinated chickens following challenge with salmonella gallinarum.a dot immunobinding assay (dia) was compared with the standard tube agglutination test (sat) for detecting antibodies in sera from vaccinated and unvaccinated chickens challenged with salmonella gallinarum var duisburg . the virulence plasmid-cured s. gallinarum vaccine was given at 7 and 21 days, and the challenge at 35 days. the dia was very sensitive and detected antibodies to high titres in challenged birds. it also detected high titres in vaccinated, unchallenged birds which failed to react ...199916147555
[specified pathogen-free poultry flocks: the current situation].before the implementation of strategies to establish specified pathogen-free commercial poultry flocks, the ultimate goals need to be identified: 1) consumer protection by minimizing the risk for zoonotic diseases and food-borne pathogens, and/or 2) animal health protection against primary and secondary pathogens. the success for the establishment of specific pathogen-free poultry flocks depends on the biological characteristics, the epidemiological distribution and the transmission route of eac ...200516218186
oral infection with the salmonella enterica serovar gallinarum 9r attenuated live vaccine as a model to characterise immunity to fowl typhoid in the chicken.salmonella enterica serovar gallinarum (s. gallinarum) is the causative agent of fowl typhoid, a severe systemic disease of chickens that results in high mortality amongst infected flocks. due to its virulence, the immune response to s. gallinarum is poorly characterised. in this study we have utilised infection by the live attenuated s. gallinarum 9r vaccine strain in inbred chickens to characterise humoral, cellular and cytokine responses to systemic salmonellosis.200516221297
effect of ochratoxin a on broiler chicks challenged with salmonella gallinarum.1. a study was conducted to evaluate the effects of ochratoxin a (oa) on broiler chicks challenged with salmonella gallinarum. 2. one hundred and seventy-six 1-d-old broiler chicks were divided into two groups of 88 chicks each, with one group fed on a control mash diet and the other given a mash diet containing 2 ppm oa. on d 14, each group was further subdivided into two groups with one group infected with s. gallinarum and the other uninfected. 3. following s. gallinarum inoculation on d 14, ...200516268101
[the antibiotic sensitivity patterns of salmonella pullorum and salmonella gallinarum in in vitro studies].the antibiotic sensitivity patterns of 100 strains of s. pullorum and 24 strains of s. gallinarum against 19 antibiotics and 3 antibiotic mixtures were determined by the broth dilution method. the strains studied were isolated from enzootic outbreaks of pullorum disease and fowl typhoid. it was found that all the strains were sensitive to ampicillin, streptomycin, neomycin, kanamycin, oxytetracycline, chloromycetin, gentamycin, signamycin, polzomycin, and neotarchocin. besides, s. pullorum was s ...197516296026
examination by elisa of sera obtained from chicken breeder and layer flocks showing evidence of fowl typhoid or pullorum disease.an indirect elisa using soluble whole cell antigen was used to screen serum samples obtained from breeder and layer flocks some of which had shown clinical or bacteriological evidence of infection with salmonella gallinarum or s. pullorum. there was good correlation between salmonella infection and the presence of serum samples showing high optical density (od) values. sera from seven flocks showing high values were retested using group d (0-1, 9, 12) lipopolysaccharide (lps) and g,m-h flagella ...199518645798
identification of salmonella gallinarum virulence genes in a chicken infection model using pcr-based signature-tagged mutagenesis.salmonella gallinarum (sg) is a non-motile host-adapted salmonella that causes fowl typhoid, a severe systemic disease responsible for significant economic losses to the poultry industry worldwide. this study describes the application of a pcr-based signature-tagged mutagenesis system to identify in vivo-essential genes of sg. ninety-six pools representing 1152 sg mutants were screened in a natural-host chicken infection model. twenty presumptive attenuated mutants were identified and examined f ...200516339940
efficacies of novel n-halamine disinfectants against salmonella and pseudomonas species.six novel n-halamine compounds of potential importance as disinfectants to the food-processing industry were tested against salmonella enteritidis, salmonella gallinarum, salmonella typhimurium, and pseudomonas fluorescens in aqueous solution. inactivation times for 10-fold reductions were determined as a function of water quality at ph 6.5 and 25 degrees c. phenol coefficients for the efficacies of the compounds against s. enteritidis have been reported also. when both stability and efficacy da ...199216348692
individual and combined effects of fusarium moniliforme culture material, containing known levels of fumonisin b1, and salmonella gallinarum infection on liver of japanese quail.three hundred day-old japanese quail (coturnix coturnix japonica) were divided into two groups with 150 quail in each group. one group was maintained on quail mash alone, while fusarium moniliforme culture material was added to quail mash in the second group from day 5 of age and was supplied at a rate of 150 ppm fumonisin b1 (fb1)/kg mash. at day 21, each group was further subdivided into two groups, yielding four groups with 75 birds apiece, which served as the control (group cx), the salmonel ...200516405006
cell-mediated and humoral immune responses to a virulent plasmid-cured mutant strain of salmonella enterica serotype gallinarum in broiler chickens.one-week-old salmonella-free broiler chicks were subcutaneously immunized and subsequently boosted two weeks later with 2x10(7)cfu 0.5ml(-1) of an 85-kb virulent plasmid-cured spectinomycin-resistant mutant strain (sg9vp(-)spc(r)) of salmonella gallinarum 9 (s. enterica serotype gallinarum 9) along with a control group of mock-immunized chickens. the chicks were subcutaneously challenged at 5 weeks of age with 5x10(10)cfu 0.5ml(-1) (5xld(50)) of wild-type s. gallinarum var. duisburg (s. enterica ...200616510254
ribotypes of salmonella enterica serovar gallinarum biovars gallinarum and pullorum.ninety-four isolates of salmonella enterica serovar gallinarum biovar pullorum and forty-one isolates of biovar gallinarum were ribotyped using the enzymes, hindiii, ecori and smai, and a digoxigenin-labelled e. coli-derived rrna probe. using hindiii, 13 profile types were observed within biovar gallinarum and 12 within biovar pullorum. the most common types accounted for 39% of biovar pullorum isolates and 47% for biovar gallinarum. ecori digests revealed two profile types within biovar pulloru ...199318671057
[rapid identification of riemerella anatipestifer on the basis of specific pcr amplifying 16s rdna].riemerella anatipestifer (ra) infection is the main disease causing severe losses in duck production. because ra is characterized more by the absence than by the presence of specific phenotypic properties and different scholar had the different results of biochemical detection, it can't always be identified quickly and correctly only by the phenotypic properties or biochemical characteristics. the research object was to develop a species specific pcr method for ra detection. because of the conse ...200616579457
pulsed-field gel electrophoresis genotyping of salmonella gallinarum and comparison with random amplified polymorphic dna.salmonella gallinarum is gram-negative bacteria that cause fowl typhoid (ft) in chickens. since the first outbreak of ft reported in 1992 in korea, it has widely spread throughout the country. today, ft is one of the most devastating diseases of poultry. the aim of the present study was to ascertain a genetic relationship among s. gallinarum isolates collected from different regions of korea over a 10-year period. we examined a total of 38 isolates of s. gallinarum obtained in 29 regions of kore ...200616584852
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