| cell-mediated and humoral immune responses of cattle to brucella abortus, mycobacterium bovis, and tetanus toxoid: evaluation of immunization and assay techniques. | a concentration of 2.5 x 10(-5) m 2-mercaptoethanol (2-me) added to the medium in lymphocyte blastogenesis assays increased both the uptake of [3h]thymidine in unstimulated lymphocyte cultures and the probability of detecting antigen-sensitized cattle. the use of 2-me did not cause lymphocytes from unsensitized cattle to react positively in blastogenesis assays. a crude brucella lysate prepared from brucella abortus strain 19 was compared with a well-characterized brucella protein allergen prepa ... | 1978 | 104645 |
| activity of bepridil and other anti-anginals on cardiovascular modifications engendered by conditioned anxiety in the dog. | the action of bepridil (5 mg/kg), perhexilene (5 mg/kg) propranolol (0.5 mg/kg), dipyridamole (0.5 mg/kg), amiodarone (10 mg/kg), pentaerythritol tetranitrate (40 microgram/kg) and nitroglycerine (40 microgram/kg) on cardiovascular manifestations of auditory-inducted emotional stress has been investigated in the conscious dog. bepridil, perhexilene and propranolol considerably reduce tachycardia provoked by the anxiogenic stimulus, with nitroglycerin exerting an attenuated effect and the others ... | 1978 | 104675 |
| high affinity uptake sites for taurine in the retina. | | 1978 | 104877 |
| bronchial mucosal permeability. | the tracheobronchial epithelium has well-developed tight junctions which on a morphologic basis should be markedly resistant to penetration by protein molecules. despite this, antigen inhalation in monkeys allergic to ascaris suum results in the rapid onset of pulmonary physiologic changes. recent studies in man and animals have shown that a substantial number of mast cells exist in the bronchial lumen and epithelium. we suggest that antigen-antibody interaction initially occurs on these superfi ... | 1979 | 104888 |
| p-hydroxyphenylacetaldoxime, an inhibitor of beta-galactosidase, produced by actinomycetes. | | 1979 | 104950 |
| transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli. | the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ... | 1979 | 104958 |
| isolation of an iron-binding compound from pseudomonas aeruginosa. | an iron-binding compound was isolated from ethyl acetate extracts of culture supernatant fluids of pseudomonas aeruginosa and was purified by successive paper and thin-layer chromatographic procedures. the purified compound was characterized by uv, visible, infrared, and fluorescence spectroscopy. the compound possesses phenolic characteristics, with little or no similarity to dihydroxybenzoates and no indication of a hydroxamate group. p. aeruginosa synthesized the compound during active growth ... | 1979 | 104968 |
| leukemia in workers exposed to ethylene oxide. | ethylene oxide, a gaseous sterilant extensively used within health care facilities, is known to be a mutagen in bacteria and in human lymphocytes. the environmental protection agency as well as the national institue of occupational safety and health have recently stipulated certain conditions for the use of ethylene oxide despite the lack of case reports or epidemiologic studies concerning carcinogenicity. we report three cases of leukemia that occurred between 1972 and 1977 in a relatively smal ... | 1979 | 105157 |
| [experimental cholelithiasis in animals]. | this paper reviews the most relevant animal-based knowledge in gallstone formation, and its contribution to our improved understanding of the human illness. the importance of diet, age, sex and hormones has been proved in both animals and humans. nevertheless, there are clear cut differences among species which make general conclusions hard to obtain. although there is no ideal experimental animal, primates and hamsters seem to be close. guinea-pigs, rats, mice, rabbits and dogs have been useful ... | 1978 | 105392 |
| [isolation of treponemas from the colon of pigs with clinical dysentery]. | optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ... | 1979 | 105448 |
| [b. cereus count in meat and dairy food products]. | studies were carried out to establish the contamination of some meat and dairy food products with b. cereus. a total of 48 heat-treated sausages (32 perishable and 16 durable) and 64 batches of pasteurized milk were sampled. it was found that 25 per cent of investigated sausage samples contained b. cereus. perishables proved to a considerable extent more frequently contaminated (33.3 per cent). the count of b. cereus in such products ranged from 10(1) to 10(3) per g. however, pasteurized milk wa ... | 1978 | 105462 |
| [some notes on the specifity of the fluorescence histochemical demonstration of gaba (author's transl)]. | the distribution of the gaba in the purkinje cells of the rat cerebellum was demonstrated by means of modified fluorescence histochemical method according to wolman (1971). three types of purkinje cells, with respect to their fluorescence intensity and gaba distribution, were evident. the gaba-fluorescence was strikingly by pharmacological pretreatment. isonicotinic acid hydrazide, penicillamine and chlorpromazine cause a granulation and marked reduction of the fluorescence compound, being norma ... | 1978 | 105559 |
| [kinetics of the serological response in the experimental primary hydatid disease of mice infected with echinococcus granulosus embryophores (author's transl)]. | a serological response kinetics study was carried out in cf-1 mice orally infected with echinococcus granulosus embryophores using immuno-electrophoresis, double diffusion, latex agglutination and passive hemagglutination. starting from the 76th day after the infection, serum antibodies reach the minimum detectable level by immunoelectrophoresis test. band number 5, now considered as specific for e. granulosus, was proved to be the first precipitating system exhibited in this test. double diffus ... | 1978 | 105664 |
| the necessity of magnesium cation for acid assistance aglycone departure in catalysis by escherichia coli (lacz) beta-galactosidase. | 1. removal of mg2+ from escherichia coli (lacz) beta-galactosidase slightly increases the rate of hydrolysis of galactosyl pyridinium salts, but decreases the rate of hydrolysis of arylgalactosides. 2. fair correlation of logkcat. and log (km) with the pka of aglycone is now observed for arglygalactosides, as well as for glycosyl pyridinium salts. 3. degalactosylation of mg2+-free enzyme is the rate-limiting step in the hydrolysis of 2,4-dinitrophenyl galactoside. 4. alpha-deuterium kinetic isot ... | 1978 | 105722 |
| mutual homology of mouse immunoglobulin gamma-chain gene sequences. | we have assessed the relative homology of mouse immunoglobulin heavy-chain gene sequence using complementary dnas (cdnas) synthesized against gamma-chain mrnas (gamma 1, gamma 2a, gamma 2b, and gamma 3) purified from mouse myelomas. cdnas complementary to the gamma-chain mrnas did not cross-hybridize with the mu- and alpha-chain mrnas, whereas they cross-hybridized to significant extents (22--66%) with the gamma-chain mrnas of other subclasses. the heterologous hybrids formed, however, melt at 5 ... | 1979 | 105754 |
| production and characterization of two hemolysins of bacillus cereus. | bacillus cereus strain b-48 produced two hemolysins with molecular weights of 52,000 (h-i) and 31,000 (h-ii). a mutant was isolated that produced only h-ii but was identical with the wild type in all other respects. we exploited this mutant to produce h-ii for study that was free of contamination by h-i. by manipulation of media composition, we produced h-i in the absence of h-ii. the hemolysins were precipitated differently by ammonium sulfate, and both exhibited the arrhenius effect when heate ... | 1978 | 105792 |
| animal models for the comparative assessment of neurotoxicity following repeated administration of vinca alkaloids. | neurotoxicity is the major side effect occurring during the clinical use of vincristine (vcr). animal models predictive of potential neurotoxicity would be very useful in the preclinical development of new vinca compounds. to simulate conditions in which neurotoxicity is produced during the clinical use of vcr, experimental animals (except the guinea pig) were given the test compounds by the iv route over a prolonged time period. doses were selected based on the production of leukopenia. vindesi ... | 1979 | 105807 |
| insulin and glucagon binding and degradation by kidney cell membranes. | the binding and degradation of insulin and glucagon to kidney cell membranes was examined. time- and temperature-dependent specific binding of [125i]iodo-insulin to kidney cell membranes was demonstrated. the membranes also degraded insulin in a time-, temperature-, and protein concentration-dependent manner. the apparent km of the degradation was 2.7 x 10(-7) m. glucagon degradation by the kidney membranes was extremely active. per milligram of protein the kidney membrane was over 20 times as a ... | 1978 | 105882 |
| testosterone and the control of gonadotropin secretion in the male rhesus monkey (macaca mulatta). | | 1978 | 105894 |
| effect of continuously increasing concentrations of plasma ketone bodies on the uptake and oxidation of glucose by muscle in man. | muscle metabolism in man was studied by measuring the arterial and deepvenous concentrations of glucose, lactate, pyruvate, free fatty acids, beta-hydroxybutyrate and aceacetoacetate, and forearm blood flow. after the subjects had fasted overnight, their arterial free fatty acid and ketone levels rose continuously during a period of 90 min, leading to increased ketone body uptake by muscle. hence, for each subject, a relation was obtained between arterial concentrations and arterial-deepvenous d ... | 1978 | 105915 |
| defective cellular immunity to gram-negative bacteria in cystic fibrosis patients. | in vitro lymphocyte responses to pseudomonas aeruginosa have been found to be impaired in cystic fibrosis patients with advanced clinical disease. the responses to klebsiella pneumoniae, serratia marcescens, and proteus mirabilis were studied in a similar group of cystic fibrosis patients and normal individuals. cystic fibrosis patients found to be unresponsive to pseudomonas were also unresponsive to klebsiella, serratia, and proteus. responsiveness to staphylococcus aureus was not impaired in ... | 1979 | 106011 |
| thyroid function studies in the nephrotic syndrome. | total serum and urinary thyroxine (t4), triiodothyronine (t3), and thyroxine-binding globulin (tbg) as well as serum free t4, thyroid-stimulating hormone (tsh), and t3 resin uptake (t3ru) were measured in seven patients with the nephrotic syndrome. the nephrotic syndrome was defined by proteinuria exceeding 3 g/24 h. all patients were clinically euthyroid. most values for total serum t4, free t4, t3, t3ru, tbg, and tsh were within normal limits. however, the mean serum t3 and tbg values were sig ... | 1979 | 106751 |
| trans-stilbene oxide: a new inducer of rat liver microsomal udp-glucuronyltransferase. | | 1979 | 106858 |
| association of beta-glucosidase with intact cells of thermoactinomyces. | the location of the b-glucosidase activity in a whole culture broth of the thermophilic organism thermoactinomyces has been studied. little beta-glucosidase activity was found in the culture filtrate, while the culture solids contained the major part of the activity of the whole culture broth. the activity does not appear to be adsorbed to the culture solids; rather there is evidence that it is an intracellular soluble enzyme(s). the ph and temperature optima for a crude beta-glucosidase prepara ... | 1979 | 106906 |
| [perhexiline-maleate-induced lipidosis in mouse spinal ganglia tissue culture. preliminary results on the acute toxicity on the drug]. | | 1978 | 106980 |
| effects of nitroglycerin on echocardiographic measurements of left ventricular wall thickness and regional myocardial performance during acute coronary ischemia. | the effects of nitroglycerin on regional left ventricular performance, assessed by echocardiographic techniques, were investigated in anesthetized, open-chest dogs during acute myocardial ischemia. during transient occlusion of the left anterior descending coronary artery, there was end-diastolic thinning and marked reduction in systolic thickening in the central ischemic zone. similar changes of lesser degree were noted in the border zone. the normal zone was unaffected. infusion of nitroglycer ... | 1979 | 106986 |
| penicillin-resistant gonorrhea. | | 1979 | 106997 |
| monooxygenase and glucuronyltransferase activities in short term cultures of isolated rat hepatocytes. | | 1979 | 107042 |
| effect of active and passive antibacterial immunization on skin allograft survival in rabbits. | the effect of active and passive antibacterial immunization on transplantation immunity was investigated in rabbits. immunization with dead bacterial vaccines prepared from e. coli, proteus mirabilis and pseudmonas aeruginosa had no effect on skin allograft survival, whereas immunization with vaccines prepared from streptococcal and staphylococcal strains shortened allograft survival. similar results were obtained with specific antisera against individual microbial species. skin allografts survi ... | 1979 | 107050 |
| carbonic anhydrase of human endometrium: biochemical & clinical aspects. | | 1978 | 107115 |
| electron capture gas-liquid chromatographic-mass spectral identification of acids produced by neisseria meningitidis in a defined medium. | the acid metabolites and the cellular fatty acids of three strains of neisseria meningitidis grown in a chemically defined liquid medium were determined with computerized frequency-pulsed electron capture gas-liquid chromatography. five acids not previously reported were subsequently identified: isobutyric, octanoic, decenoic (c10:1), dodecenoic (c12:1), and tetradecenoic (c14:1). these acids were produced during active metabolism and were not detected as cellular constituents. the frequency-pul ... | 1979 | 107187 |
| [antibacterial activity of gentamicin, tobramycin, amikacin, and sisomicin in vitro (author's transl)]. | | 1978 | 107386 |
| [stability of streptomyces griseus carboxypeptidase]. | carboxypeptidase of str. griseus strain 32-13 is stable during storage at a temperature of 10-15 degrees c. carbohydrates and glycerol in a concentration of 40-80% as well as 0.01 m of l-amino acid prevent the enzyme from thermoinactivation. anions, such as phosphate, borate, carbonate and acetate, have their inhibitory effect on carboxypeptidase. after the effect of chelate reagents the enzyme activity restores completely only in the presence of co2+ and partially in the presence of ni2+ greate ... | 1978 | 107641 |
| pituitary-thyroid function in spironolactone treated hypertensive women. | four weeks high dose spironolactone treatment (aldactone searle, 100 mg q. i. d.) significantly enhanced the tsh (delta max. 8.5 +/- 4.1 vs. 4.6 +/- 3.1 microunits/ml, p less than 0.05) and t3 (delta max. 32 +/- 27 vs. 11 +/- 16 ng/100 ml, p less than 0.05) responses to an intravenous trh/lh-rh bolus injection in 6 eumenorrhoeic euthyroid hypertensive women, without affecting basal serum tsh, t3 or t4 levels or the basal and stimulated lh, fsh and prolactin values (p greater than 0.10). the mean ... | 1979 | 107696 |
| constant and flickering light stimulations produce similar effects on rna content in visual cells. | adult male rats were illuminated for 2 h with a constant or flickering light of 40 lx intensity; frequency of flickering was 2 hz. by means of two-wave-length visible cytospectrophotometry of gallocyanin-stained sections, it was shown that the light stimulation resulted in a marked rna accumulation in retina ganglion neurons and in the neurons of all the cell layers of visual cortex (with the only exception of the layer vi). in the cells of perineuronal glia, a decrease of the rna content per ce ... | 1978 | 107713 |
| iron and the antibotulinal efficacy of nitrite. | combination of nitrite, isoascorbate, and ethylenediaminetetraacetic acid were compared for their antibotulinal efficacy in perishable canned cured meat. a dose response relationship of available iron to the antibotulinal efficacy of nitrite was demonstrated. | 1979 | 107856 |
| preparation of monospecific antibodies against two forms of rat liver cytochrome p-450 and quantitation of these antigens in microsomes. | | 1979 | 107862 |
| isolation of listeria monocytogenes from animals in the sudan. | | 1979 | 107996 |
| transport of neisseria meningitidis cultures: growth-supporting media and freezing as an alternative. | the abilities of transgrow (tg), thayer-martin (tm), and new york city (nyc) solid media to maintain the viability of 12 strains of neisseria meningitidis under various controlled conditions were assessed. the effects of charcoal impregnation of swabs, temperature, and an enriched co2 atmosphere were examined with holding for up to 21 days. recovery from samples held at 35 degrees c was, in almost all instances, greater than at 22 or 4 degrees c. a strong requirement for added co2 was demonstrat ... | 1979 | 108008 |
| limitations of aeruginocine typing of ps. aeruginosa as an epidemiological marker. | | 1978 | 108218 |
| [investigations on the aminoglycoside inactivating enzyme aac-(6') iv (author's transl)]. | in serratia marcescens and pseudomonas aeruginosa from clinical specimen acetyltransferase aac-(6') iv leads to varying phaenotypically expressed resistance to aminoglycoside antibiotics. the substrate profile and the rate of inactivation of the enzymes investigated are almost identical with each other and with the enzyme of p. aeruginosa gn 315. the enzymes cannot be associated with r factors by the standard methods for conjugation, transformation, density gradient centrifugation and agarose g ... | 1979 | 108221 |
| antibiotic resistance in various medical services. | the analysis of differences in occurrence of antibiotic-resistant strains belonging to ten species of "problem" bacteria was performed by means of a computer program in more than 150,000 bacterial strains isolated in slovakia in 1977. in all respects, strains isolated in general medical practice were considerably more sensitive to classical as well as to newer antibiotics. marked differences in resistance within individual medical services could be a reflection of both quantitative and qualitati ... | 1979 | 108222 |
| demonstration and characterization of specific binding sites for factor viii/von willebrand factor on human platelets. | the presence of specific factor viii/von willebrand factor (fviii/vwf) binding sites on human platelets has been demonstrated by using 125i-fviii/vwf and washed human platelets. binding is ristocetin-dependent and increases in proportion to the concentration of ristocetin from 0.2 to 1 mg/ml. binding of 125i-fviii/vwf to platelets can be competitively inhibited by unlabeled human or bovine fviii/vwf, but not by human thrombin, fibrinogen, alpha 2-macroglobulin, equine collagen, or a lectin of ri ... | 1979 | 108291 |
| minimal antibiotic concentrations of aminoglycosides and beta-lactam antibiotics for some gram-negative bacilli and gram-positive cocci. | the minimal antibiotic concentration (mac) is the lowest concentration of an antibacterial agent that produces a decrease of 1 log in the number of organisms/ml as compared with a control culture in drug-free medium. various gram-negative bacilli and gram-positive cocci were grown in the presence of amikacin, gentamicin, tobramycin, ampicillin, amoxicillin, oxacillin, carbenicillin, ticarcillin, and cefamandole at concentrations varying from eight times the minimal inhibitory concentration (mic) ... | 1979 | 108345 |
| biosynthesis of glycosylated hemoglobins in the monkey. | we have investigated the in vivo biosynthesis of the minor hemoglobin components in the rhesus monkey. the elution profile of rhesus hemolysate on biorex 70 cation exchange resin was analogous to that of human hemolysate. the rhesus hb alc peak was identified with the tba test, which revealed a carbohydrate content identical to that of human hb a1c. a rhesus monkey was injected with autologous 55fe-bound transferrin, and the specific activity of each of the minor and major components was followe ... | 1979 | 108346 |
| epidemic occurrence of neonatal necrotizing enterocolitis. | in case-control studies of three epidemics of neonatal necrotizing enterocolitis (nec) in three different high-risk nurseries in three states, no particular risk factor was associated with affected infants or their mothers. epidemic cases had higher birth weights and apgar scores and fewer perinatal difficulties than those previously reported for sporadic cases. seven infants fed primarily breast milk were not protected against disease. early antibiotic therapy was associated with a significantl ... | 1979 | 108997 |
| a new principle of injectable depot contraceptives. i drug selection and studies in monkeys. | the aim of the investigations was to develop a long-acting depot contraceptive on the basis of norethisterone or levonorgestrel. disappointing results with levonorgestrel nonanoate and levonorgestrel undecylate showed that elongation of the fatty acid, esterified with the steroid, decreased the bioavailability of the latter due to incomplete hydrolysis of the ester. therefore, several new compounds were synthesized which contained a bifunctional molecule between the steroid and the fatty acid. i ... | 1979 | 110513 |
| [antibacterial in-vitro activity of pipemidic acid and nalidixic acid (author's transl)]. | the in-vitro activity of nalidixic acid and pipemidic acid was investigated in a combined study in 450 freshly isolated bacterial strains using the agar dilution test. gram-positive cocci were resistant to both substances except for a few pipemidic acid sensitive staphylococci. both substances had good antibacterial activity in the gram-negative spectrum. however, the mic values of pipemidic acid were generally clearly lower than those of nalidixic acid. only pipemidic acid showed activity again ... | 1979 | 110574 |
| [adjuvant effect of tuberculin on sensitivity to gram negative bacteria]. | | 1979 | 111205 |
| [rapid methods useful for determination of streptococcal groups with special reference to streptococci of groups b and d]. | a study was carried out on the efficiency of certain rapid methods for the identification of streptococcal groups, especially groups b and d, methods based upon physiologic properties such as hippurate hydrolysis, production of pigment, esculin hydrolysis, tolerance to salt and bilolysis, associated with the classical tests, for instance the hemolysis type and sensitivity to bacitracin and optochin. by these tests, accessible to hospital laboratories, a number of 3083 streptococcal strains were ... | 1979 | 111335 |
| in vitro activity of piperacillin compared with that of carbenicillin, ticarcillin, ampicillin, cephalothin, and cefamandole against pseudomonas aeruginosa and enterobacteriaceae. | piperacillin (t-1220), a semisynthetic derivative of aminobenzylpenicillin, was more active than either carbenicillin or ticarcillin against pseudomonas aeruginosa; over 60% of isolates were inhibited at a concentration of 6.3 mug/ml. piperacillin was bactericidal for 84% of pseudomonas strains at 100 mug/ml, carbenicillin killed 60%, and ticarcillin killed 68% at that concentration. piperacillin was also more active than the other penicillins against isolates of escherichia coli, enterobacter, ... | 1979 | 111609 |
| comparative in vitro activity of azlocillin, ampicillin, mezlocillin, piperacillin, and ticarcillin, alone and in combination with an aminoglycoside. | the in vitro activities of the newer semisynthetic penicillins azlocillin, mezlocillin, and piperacillin were compared with those of ampicillin and ticarcillin by using 290 clinical laboratory isolates. piperacillin and mezlocillin were the most active against escherichia coli, proteus mirabilis, klebsiella spp., and enterobacter spp. when pseudomonas aeruginosa was tested, piperacillin and azlocillin were more active than either mezlocillin or ticarcillin. streptococcus pneumoniae and haemophil ... | 1979 | 111616 |
| a comparison of the in vitro activity of tobramycin and gentamicin against 6,042 clinical isolates. | 6,042 strains of staphylococcus aureus, streptococcus faecalis, proteus mirabilis, escherichia coli, klebsiella sp. and pseudomonas sp. were tested against tobramycin and gentamicin. among all species studies, except pseudomonas sp, gentamicin turned out to be slightly superior to tobramycin. | 1979 | 113179 |
| [effect of combination of hetacillin, cephapirin and amikacin (author's transl)]. | with the investigations of the effect of combination of hetacillin, cephapirin and amikacin, following experimental results were obtained; 1. effect of combination of amikacin, cephapirin and hetacillin was differed depending upon the genus and species of bacteria. however, in general, in the cases showing effective results there was a relation in the concentration of these antibiotics. with strains of escherichia coli amikacin and cephapirin was cooperative, but not with other combinations and ... | 1979 | 114683 |
| pharmacological modification of experimental tardive dyskinesia. | cebus apella monkeys subjected to chronic haloperidol administration develop neurologic disturbances very similar to neuroleptic-induced acute dystonia human beings. after varying lengths of time, certain monkeys develop a prolonged dyskinetic syndrome resembling tardive dyskinesia (td), as seen clinically. two monkeys with signs of td were given single intramuscular injections of various compounds with known effects on the catecholaminergic, cholinergic, serotoninergic and gaba-ergic neurotrans ... | 1979 | 115227 |
| medical ethics and controlled clinical trials. | the controlled clinical trial is a relatively new phenomenon; the first large clinical trial on evaluation of streptomycin in therapy of pulmonary tuberculosis occurred in 1946. in such a study, two or more groups of patients with similar characteristics are chosen by random allocation to receive one or more therapies. the essential ethical dilemma is based on the risk-benefit ratio of the new therapy. ethical factors that must be considered in the design of controlled clinical trials include pr ... | 1979 | 115367 |
| renal malakoplakia with renal vein thrombosis. | | 1979 | 116513 |
| [epidemiology of hospital-acquired urinary tract infections (author's transl)]. | among hospital-acquired infections, urinary tract infections are the widest spread. the most common pathogenic agents are e. coli, enterococci and proteus mirabilis. indwelling bladder catheters account for about 70% of infections. suprapubic urine drainage can reduce the incidence of urinary tract infections by approximatively 50%. | 1979 | 117318 |
| an in vitro chromosome assay using cultured rat-liver cells. | an in vitro chromosome assay has been developed which utilises an epithelial-like cell line derived from rat liver. the cell line, designed rl1, retains sufficient metabolic enzyme activity to detect chromosome damage induced by a variety of chemical mutagens and carcinogens without the incorporation of an extrinsic metabolising system. the cells are grown on standard glass microscope slides, exposed to the test chemical and processed in situ for metaphase analysis. in a small validation study, ... | 1979 | 117353 |
| [etiotropic treatment of patients with calculous pyelonephritis]. | | 1979 | 118533 |
| in vitro and in vivo laboratory studies on three hydroxyiminophenylacetyl cephalosporins with particular reference to sk&f 80303, an unusually long-acting cephalosporin. | three cephalosporins with 7-(2-hydroxyiminophenylacetamido) side chains (sk&f 79433, 80000 and 80303), differing in their 3-substituents, exhibited similar broad-spectrum antibacterial activity in vitro against strains of staphylococcus aureus, streptococcus faecalis and various gram-negative bacilli. all three were active in vivo (s.c., mouse) against s. aureus, escherichia coli or klebsiella pneumoniae, but they differed significantly in serum pharmacokinetic profiles. sk&f 80303 produced high ... | 1979 | 118958 |
| ["in vitro" activity of a new cephalosporin (cgp-7174-e or cefsulodin) against "pseudomonas aeruginosa" (author's transl)]. | the minimal inhibitory concentration (mic) of four betalactamins (carbenicillin, ticarcillin, piperacillin and (cgp-7174-e) against 109 clinical isolates of pseudomonas aeruginosa was determined by an agar dilution method. cgp-7174-e was the most active (mic 3.4 micrograms/ml), followed in descending order by piperacillin (mic 4.9 micrograms/ml), ticarcillin (mic 22 micrograms/ml) and carbenicillin (mic 36 microgram/ml). high correlation was found between the mic of these betalactamins (r greate ... | 1979 | 120122 |
| immunization against experimental trypanosoma cruzi infection: inherent difficulties of a uniform comparative evaluation of antigenic preparations. | | 1979 | 120626 |
| [effect of varying amounts of lysine in the ration on chickens]. | the effect of varying lysine quantities given in the daily ration to 20 day-old chickens was studied for a 40 day-period. highest body gain (157% as compared to the control group) was observed in group i (0.20% lysine). two and four per cent of lysine in the ration reduced the basic body gain by 69.7% and 66.9% respectively. the level of calcium, blood sugar, carotene and vitamin a rose considerably, while phosphorus and magnesium content was reduced. sgot and sgtp values remained without signif ... | 1979 | 120632 |
| the effect of lampit on trypanosoma cruzi in mice organs and in the bloodstream. | mice infected with bloodstream forms of trypanosoma cruzi were treated with an active nitrofuran compound (nifurtimox, lampit). determination of the number of intracellular forms of t. cruzi in the liver and the spleen of control and lampit-treated mice showed that the drug induced a decrease in the number of parasites inside the cells. a decrease in the number of bloodstream forms was also observed. ultrastructural observations showed that lampit induces several alterations in t. cruzi, the mos ... | 1979 | 120645 |
| [antibacterial activity of mezlocillin and azlocillin/a survey (author's transl)]. | a review of the antibacterial activity of 6-[(r)-2-[3-methylsulfonyl-2-oxo-imidazolidine-carboxamido]-2-phenyl-acetamido]-penicillanic acid sodium salt (mezlocillin, baypen) and 6-[(r)-2-(2-oxo-imidazolidine-1-carboxamido)-2-phenyl-acetamido]-penicillanic acid sodium salt (azlocillin, securopen) is given on the basis of extensive in vitro studies. the action of each of the preparations is compared with that of important antibiotics of other substance classes from the group of penicillins and cep ... | 1979 | 120745 |
| the effect of prophylactic tinidazole on the anaerobic vaginal flora in patients undergoing gynaecological surgery. | the effects of prophylactic tinidazole on the vaginal carrier state of anaerobic bacteria and the development of postoperative infections were studied in 50 patients undergoing gynaecological surgery. the results are based on a double-blind trial, using tinidazole (fasigyn; pfizer) and placebo. although no postoperative infections other than urinary tract infections were observed in our series, this study shows conclusively that the isolation of anerobic bacteria from the vagina decreased marked ... | 1979 | 121639 |
| [the antibacterial efficacy of cefaclor in routine testing of clinical material from two berlin hospitals (author's transl)]. | the agar diffusion method was used to test the antibacterial efficacy of cefaclor against bacterial strains isolated routinely from patients in two hospitals in berlin. a comparison was made with the efficacy of oxacillin, azlocillin, amikacin, gentamicin, ampicillin, co-trimoxazole, tetracycline, penicillin, cefazolin, nalidixic acid and nitrofurantoin. a total of 1235 strains of staphylococcus aureus, enterococci, escherichia coli, klebsiella, enterobacter, proteus species, citrobacter and pse ... | 1979 | 121730 |
| [frequency and antibiotic resistance of bacteria isolated from urine cultures]. | the aa report the data pertinent to the urine culture performed during a year in the sanitary and prophylactic laboratory of the district of l'aquila. in urine samples with number of bacteria > 10(5)/ml have been found e. coli (49%), proteus mirabilis (32%), pseudomonas aeruginosa (12%), staphylococcus aureus (5%), proteus rettgeri (1%), enterococco (1%). the data are also reported concerning germ resistance to several antibiotics. | 1979 | 121968 |
| in vitro activity of piperacillin, a new semisynthetic penicillin with an unusually broad spectrum of activity. | the in vitro activity of piperacillin (t-1220), a new semisynthetic derivative of aminobenzylpenicillin, was investigated. the majority of streptococci and pneumococci were inhibited by 0.12 micrograms/ml; the staphylococci and enterococci were inhibited by 2 micrograms/ml. piperacillin was slightly more active against neisseria and haemophilus influenzae than was ampicillin. piperacillin was active against all members of the enterobacteriaceae including the klebsiella, 58% of which were inhibit ... | 1978 | 122518 |
| totally synthetic medium for susceptibility testing. | the results of disk diffusion and plate dilution susceptibility testing of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and proteus mirabilis on media gelled with agar-agar or with a synthetic hydrogel were compared. synthetic hydrogel can be combined with a totally defined synthetic amino acid medium to yield a reproducible, totally defined, synthetic solid medium without the antagonistic or booster effects of some components of agar. such a medium could be used as a referen ... | 1978 | 122521 |
| [gram-negative infection of the foot]. | | 1979 | 122538 |
| [the inhibition of various bacterial proteinases with natural proteinase inhibitiors(author's transl)]. | proteus mirabilis, citrobacter intermedius, staphylococcus aureus haemolyticus and staphylococcus albus produce extracellualr proteinases with maximum activity at ph 7.8. these proteinases are inhibited practically completely by human and animal blood serum. this inhibitory activity of human serum is caused by alpha2-macroglobulin. ovomucoid showed nearly the same inhibitory activity as alpha2-macroglobulin. | 1975 | 123295 |
| [effects of caudate nucleus stimulations on the primary and secondary endings activity of soleus muscle spindle]. | effects of repetitive stimulation of the contralateral caudate nucleus on the static discharge and dynamic sensitivity of soleus muscle spindle primary and secondary endings were studied in cats anaesthetized with halothane (fluothane). by progressive increasing the depth of anaesthesia two different fusimotor effects could be observed. 1. a static facilitatory effect; under light anaesthesia the static discharge of primary and secondary endings were strongly increased. the response of the prima ... | 1975 | 123860 |
| mutagenicity of nitrofuran derivatives, including furylfuramide, a food preservative. | | 1975 | 124819 |
| comparison of the effect of various antisera and cobra venom factor on inflammatory reactions in guinea-pig skin. ii. the arthus reaction and the local shwartzman reaction. | the ability of antisera to guinea-pig c3 to inhibit the arthus and local shwartzman reactions was studied. they were found to reduce the non-haemorrhagic component of the active and reversed passive arthus reactions and to delay the appearance of the haemorrhage in the active arthus reaction. cobra venom factor, however, had no effect on the non-haemorrhagic components of these reactions and only delayed the appearance of the haemorrhage of the active arthus reaction. there appeared to be a corr ... | 1975 | 125318 |
| the effect of uncouplers on catecholamine incorporation by vesicles of chromaffin granules. | it is shown that uncouplers inhibit the incorporation of catecholamines by vesicles of chromaffin granules in parallel with their stimulatory effect on the membrane-bound adenosine triphosphatase. | 1975 | 125589 |
| [some regularities of the regeneration of the musculature of internal organs]. | the paper summarizes the data of the author and his co-workers on regulation of the musculature of vessels, digestive tract, uterus, ureters and urinary bladder. the smooth muscle cells have different degrees of differentiation. the muscular tissue of the vessels is most differentiated. the uterus musculature is very plastical. after injury mature myocytes are the first to undergo destruction. the intermedial substance is more stable. myoblasts, young elements of the fibroblast row and the suben ... | 1975 | 126673 |
| amino-acid sequence of the cyanogen-bromide fragment from human plasminogen that forms the linkage between the plasmin chains. | the complete amino acid sequence of a cyanogen bromide fragment (122 residues) obtained from plasminogen is described. this fragment forms the overlap between heavy (a) and light (b) chains of human plasmin. the particular arginyl-valyl bond cleaved in the second step of the activation process is shown to be arg98-val99 in this fragment. this site is not very similar to the one in the nh2-terminal part of the molecule (arg68-met69). remarkable homologies with the 'triple loops' ('kringle structu ... | 1975 | 126863 |
| characterization of 5.8s ribosomal ribonucleic acid in neurospora crassa. | neurospora crassa ribosomes contain a species of ribonucleic acid (rna) of molecular weight 54,000, similar to 5.8s ribosomal rna previously described for other eukaryotic organisms. the 5.8s rna from n. crassa was found to be released by heat treatment at 60 c from 25s ribosomal rna but not from 18s ribosomal rna. the base composition of n. crassa 5.8s rna was similar to that of 5.8s rna from saccharomyces cerevisiae, but differed from animal 5.8s rna. during the course of this study, it was di ... | 1975 | 126993 |
| [clinical aspects of chromosomal aberrations - problems of semiotics and nosography]. | despite intense clinical and cytogenetic research for more than 15 years we are far from knowing any definite relations between karyotype and phenotype. both cytogeneticists and clinicians are working on the methodological improvements which are still necessary. in the methods of clinical recording there is still very great weakness of exact documentation of findings. above all there is a lack of reproducible qualitative, and especially quantitative standards which constitute the basis of any pr ... | 1975 | 127146 |
| the hurler syndrome: treatment of cultured hurler fibroblasts with normal human serum. | prolonged replacement of fetal calf serum by normal human serum for the enrichment of medium during tissue culture of hurler fibroblasts resulted in increased acid mucopolysaccharides in the cells and in the medium. the predominant intracellular mucopolysaccharide had the characteristics of dermatan sulfate when hurler cells were treated with either serum. normal human serum contains a nonspecific coreective factor capable of augmenting the loss of 35so4-amps from hurler cells, but not from norm ... | 1975 | 127180 |
| [muscular clearance after administration of phenoxybenzamine in shock]. | the authors made an experimental investigation in the dog concerning the evolution of the muscular clearance of phenoxybenzamine in the course of de-shocking procedures. the animals were shocked by hemorrhage and were treated by perfusion of volemic mass and administration of phenoxybenzamine. this substance was flowed in paralell with the hemodynamic parameters and the rhythm of the diuresis. it was noted that the direction and the amplitude of changes concerning the half-life of na131i do not ... | 1975 | 127228 |
| [experiments and theoretical cell kinetic calculations on the problem of in vivo synchronization with vincristine in l 1210 ascites tumor cells and crypt cells of the mouse (author's transl)]. | experiments are described on synchronizing l 1210 ascites tumor cells and jejunal crypt cells of the mouse with vincristine. in both cases the percentage of mitoses increases to a peak value 4 hrs after application of vincristine and decreases during the following 8 hrs ("releasing time") to the level of the normal mitotic index. following this first mitotic peak no futher peak of neither the mitotic nor the labeling index was observed during 48 hrs in l 1210 ascites tumor cells and during 38 hr ... | 1975 | 127443 |
| [change of the prevalent subtype of hepatitis b antigen in acute hepatitis b infections (author's transl)]. | subtyping of hepatitis b antigen (hbs ag) in patients with acute hepatitis b revealed subtype ay in 75 percent while subtype ad was much more common in chronic hepatitis b infections: 81 percent of hbs ag positive blood donors and 76 percent of patients with hbs ag positive chronic aggressive hepatitis revealed subtype ad. in contrast to blood donors and chronic hepatitis patients, a change of the prevalent subtype was noted in acute hepatitis patients between 1970 and 1974. before may 1972, sub ... | 1975 | 127476 |
| reaction of yeast phosphofructokinase with succinic and maleic anhydride. | modification of yeast phosphofructokinase by succinic and maleic anhydride influences the catalytic activity and the allosteric behaviour of the enzyme. depending on the degree of succinylation and maleinylation a decrease of maximum activity, an increase of the apparent affinity for fructose-6-phosphate, a decrease of the hill-coefficient and a diminution of atp-inhibition are observed. up to about 40% of the lysyl residues could be succinylated without dissociation of the hexameric protein, ho ... | 1975 | 127493 |
| [changes in the lungs and the mass of the right ventricle of the heart in chronic hypoxia (experimental-morphological study)]. | in experiments on 105 white rats using histological, histochemical, and morphometric methods the state of the lungs following daily "rises" in a pressure chamber to the "hight" of 5000--9000 m at verious time intervals--from 1 day to 9 weeks was studied. in 18 animals "ascents" were preceded by sinistral pulmonectomy. in parallel, the heart was investigated by the method of separate weighing. at initial stages changes in the lungs were characterized predominantly by impairment of the blood- and ... | 1975 | 128341 |
| [effect of air-pollution on man (author's transl)]. | | 1975 | 128718 |
| [g. f. lang - prominent soviet scientist, clinician and public man (on the centenary of his birth)]. | | 1975 | 130732 |
| treponemal antigen in immunopathogenesis of syphilitic glomerulonephritis. | a patient with syphilitic glomerulonephritis had a renal biopsy and was treated for secondary syphilis. light, electron, and immunofluorescence microscopic studies revealed an acute proliferative glomerulonephritis with subepithelial, intramembranous, and subendothelial immune complex deposits containing igg, iga, igm, c4, and c3. similar local deposits containing predominantly igm were noted in areas of mesangial proliferation. indirect fluorescent antibody studies employing rabbit treponemal a ... | 1976 | 130804 |
| isolation of ionophores from ion-transport systems. | | 1975 | 130822 |
| [calcium transport and atpase activity of sarcoplasmic reticulum in normal and denervated rabbit muscles]. | the properties of sarcomplasmic reticulum ca-pump from normal and denervated rabbit muscles were investigated. ca+2 ion transport in denervated muscle reticulum was subject to michaelis-menten kinetics. the rate of fast ca2+ outflux from the vesicles was enhanced after denervation; this caused a decrease in the transport efficiency and an increase of the "basic" atp-ase. at the same time the rate of ca2+ accumulation and the ca-atp-ase transport activity were enhances by a factor of 1.5. kinetic ... | 1976 | 132975 |
| letter: offspring of females with trisomy 21. | | 1976 | 133007 |
| the effect of increased brain gaba produced by amino-oxyacetic acid on arousal in rats. | previous work has reported behavioral arousal in the rat to be inversely related to cortical gaba production. therefore the effects of an increase in brain gaba levels, induced by amino-oxyacetic acid, on measures of behavioural arousal such as rearing and ambulation were examined. the increase in gaba was immediately associated with decreased rearing, however the behaviour was later indistinguishable from control values while the levels of brain gaba remained raised. it was suggested that the r ... | 1976 | 134388 |
| [influence of a lathyric agent and of hypercholesterolemic serum on cell cultures of fetal rabbit aorta]. | cell cultures of foetal rabbit aorta are cultivated with a lathyric agent (beta-amino-propio-nitrile) or with an hypercholesterolemic serum; if morphological features, in these two cases, correspond with modifications observed, in vivo, when adult rabbits are respectively submitted to the same treatment, enzymatic activities of collagen metabolism vary in opposite way. therefore, the influence of different parameters to be studied on vascular cell functions become easier. | 1976 | 134801 |
| localization on mitochondrial dna of mutations leading to a loss of rutamycin-sensitive adenosine triphosphatase. | four cytoplasmic mutants of saccharomyces cerevisiae showing loss of mitochondrial rutamycin-sensitive atpase activity but having significant cytochrome oxidase and nadh-cytochrome c reductase have been isolated. genetic studies indicate the mutations to be closely linked to each other and have been assigned to a new locus, pho1. the mutations show a low frequency of recombination with the ol12 locus, suggesting a linkage to this marker. they are not, however, linked to the oli1 locus. linkage o ... | 1976 | 134892 |
| the acid mucopolysaccharides (glycosaminoglycans) in secondary cultures of embryonic rat fibroblasts. | in secondary cultures of embryonic rat fibroblasts the protein-free acid mucopolysaccharides were separated by cetylpyridinium chloride fractionation and identified afterwards. the following acid mucopolysaccharides were found: hyaluronic acid, chondroitin, 2 heparan sulphate fractions, chondroitin-4-sulphate, chondroitin-6-sulphate, and dermatan sulphate. | 1975 | 134903 |
| biochemical variations in the matrix of human tendons in relation to age and pathological conditions. | with increasing age, the human tendon shows an increase of collagen and a diminution of mucopolysaccharides and glycoproteins. similar findings have recently been reported by other authors in the tendons of mammals. fragments of tendon affected by tendinosis, obtained dlring suture and tenoplasty operations, were also examined. marked diminution of collagen and an increase of acid mucopolysaccharides and structural glycoproteins were found as compared with the control tendon. | 1975 | 134980 |
| cardiovascular adaptations to physical training. | | 1977 | 139843 |
| neonatal islet cell transplantation in the diabetic rat: effect on hepatic enzyme activity and glucose homeostasis. | intraperitoneal transplantation of collagenase-digested, isogeneic, neonatal rat pancreatic tissue successfully reversed streptozotocin-induced diabetes in 77% of recipients. the low serum immunoreactive insulin, hyperglycaemia, glycosuria and weight loss, characteristic of the diabetic animal, were corrected and the reduced activities of hepatic glucokinase and pyruvate kinase, and the low glycogen concentration of the liver of diabetic rats were restored to normal. forty-three per cent of the ... | 1977 | 142794 |