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the antimicrobial effect of acetic acid--an alternative to common local antiseptics?acetic acid has been commonly used in medicine for more than 6000 years for the disinfection of wounds and especially as an antiseptic agent in the treatment and prophylaxis of the plague. the main goal of this study was to prove the suitability of acetic acid, in low concentration of 3%, as a local antiseptic agent, especially for use in salvage procedures in problematic infections caused by organisms such as proteus vulgaris, acinetobacter baumannii or pseudomonas aeruginosa. this study was de ...200919286325
micrornas and genomic variations: from proteus tricks to prometheus gift.micrornas (mirnas) are small non-coding rnas with regulatory functions. mirnas are aberrantly expressed in almost all human cancers, leading to abnormal levels of target genes. recently, an increasing number of studies have addressed whether genomic variations including germ line or somatic mutations and single-nucleotide polymorphisms can count for mirna abnormal expression by altering their biogenesis and/or affect the ability of mirnas to bind to target messenger rnas. here, we provide an ext ...200919293341
antibiotic susceptibility pattern of staphylococcus species isolated from telephone receivers.microorganisms are transferred to everyday objects from the environment and infected individuals. pathogenic microbes are transmissible from the air, skin, hands and other interpersonal contacts and cause diseases most of the time. this study centres on the microbial assessment of telephone receivers, their ability to transfer bacterial infections and the isolation of these microorganisms from receivers, with an emphasis on staphylococcus aureus and its antibiotic susceptibility pattern.200919296038
novel ctx-m {beta}-lactamase genotype distribution and spread into multiple species of enterobacteriaceae in changsha, southern china.the aim of this study was to undertake a survey of the occurrence of ctx-m and shv extended-spectrum beta-lactamase (esbl) genotypes in enterobacteriaceae from hunan province, china.200919297379
tamm-horsfall protein protects against urinary tract infection by proteus mirabilis.proteus mirabilis is a common cause of urinary tract infection. we determined the role of tamm-horsfall protein as a host defense factor against the cystitis and pyelonephritis caused by p. mirabilis.200919303096
growth inhibition of foodborne and pathogenic bacteria by conjugated linoleic acid.the influence of conjugated linoleic acid (cla) on the growth of some foodborne and pathogenic bacteria was examined. a potassium salt of cla (cla-k) was tested against three gram-positive strains ( bacillus cereus , staphylococcus aureus , and streptococcus mutans ) and five gram-negative strains ( pseudomonas aeruginosa , salmonella typhimurium , vibrio parahemolyticus , klebsiella pneumoniae , and proteus mirabilis ). cla-k-mediated growth inhibition was evident for all tested strains, partic ...200919317483
16s-23s rdna internal transcribed spacer regions in four proteus species.proteus is a gram-negative, facultative anaerobic bacterium. in this study, 813 proteus 16s-23s rdna internal transcribed spacer (its) sequences were determined from 46 proteus strains, including 388 its from 22 p. mirabilis strains, 211 its from 12 p. vulgaris strains, 169 its from 10 p. penneri strains, and 45 its from 2 p. myxofaciens strains. the proteus strains carry mainly two types of its, its(glu) (containing trna(glu (uuc)) gene) and its(ile+ala) (containing trna(ile (gau)) and trna(ala ...200919318046
laser interferometric and cultivation methods for measurement of colistin/ampicilin and saponin interactions with smooth and rough of proteus mirabilis lipopolysaccharides and cells.laser interferometry is commonly used in permeability studies of soluble substances. in this study a modification that allowed testing partially insoluble mixtures is presented. the modification relies on the measurement of diffusion from 1% agarose gel. as a model for this study, two proteus mirabilis strains were used that differ in polysaccharide content: smooth p. mirabilis s1959 strain and its re-type mutant, strain r45. by laser interferometry and precipitation it is shown that r45 lipopol ...200919318050
prevalence of multiresistant gram-negative organisms in a tertiary hospital in mwanza, tanzania.antimicrobial resistance is fast becoming a global concern with rapid increases in multidrug-resistant gram negative organisms. the prevalence of extended spectrum beta-lactamase (esbl)-producing clinical isolates increases the burden on implementing infectious disease management in low socio-economic regions. as incidence can vary widely between regions, this study was done to determine resistance patterns of gram-negative organisms at bugando medical center, a tertiary hospital in mwanza, tanz ...200919323805
eca-immunogenicity of proteus mirabilis strains.bacteria of the genus proteus are opportunistic pathogens and cause mainly urinary tract infections. they also play a role in the pathogenesis of reactive arthritis (ra). patients suffering from yersinia-triggered ra often carry high titers of antibodies specific to enterobacterial common antigen (eca). the immunogenicity of eca has not received much attention thus far and studies have focused mainly on the eca of escherichia coli and yersinia enterocolitica. in this paper the eca-immunogenicity ...200919333729
folate, cancer risk, and the greek god, proteus: a tale of two chameleons.evidence indicates that an abundant intake of foodstuffs rich in folate conveys protection against the development of colorectal cancer, and perhaps some other common cancers as well. the issue is complex, however, since some observations in animal and human studies demonstrate that an overly abundant intake of folate among those who harbor existing foci of neoplasia might instead produce a paradoxical promotion of tumorigenesis. the pharmaceutical form of the vitamin, folic acid, might affect t ...200919335714
lysozyme catalyzes the formation of antimicrobial silver nanoparticles.hen egg white lysozyme acted as the sole reducing agent and catalyzed the formation of silver nanoparticles in the presence of light. stable silver colloids formed after mixing lysozyme and silver acetate in methanol and the resulting nanoparticles were concentrated and transferred to aqueous solution without any significant changes in physical properties. activity and antimicrobial assays demonstrated lysozyme-silver nanoparticles retained the hydrolase function of the enzyme and were effective ...200919344124
colonization with multidrug-resistant gram-negative bacteria: prolonged duration and frequent cocolonization.the characteristics of colonization with multidrug-resistant gram-negative bacteria (mdrgnb) in the gastrointestinal tract have not been well defined. characterizing the duration of colonization, clearance of colonization, and frequency of cocolonization would provide important information for the development of interventions targeting the prevention of mdrgnb spread.200919348593
in vitro evaluation of chondroitin sulphate-chitosan microspheres as carrier for the delivery of proteins.a novel formulation based on chondroitin sulphate/chitosan microspheres (cs/ch) has been investigated for oral delivery of macromolecules using ovalbumin as the model protein (ova). the microspheres were prepared by a new emulsion-complex coacervation method. physico-chemical properties of the polymers constituting microparticulate matrix were investigated by ir, dsc, tga and x-ray diffraction analyses. in vitro tests were performed to evaluate the drug delivery system degradation and the protei ...200918855198
nationwide surveillance of antimicrobial resistance among enterobacteriaceae in intensive care units in taiwan.to determine the antimicrobial resistance profiles among clinical isolates of enterobacteriaceae in taiwanese intensive care units (icus), a national surveillance of antibiotic resistance among important enterobacteriaceae was conducted from september 2005 through november 2005 at the icus of ten major teaching hospitals in taiwan. a total of 574 enterobacteriaceae isolates recovered from various clinical samples of our icu patients were submitted for in vitro test. minimum inhibitory concentrat ...200918716805
synthesis, structure and biological activity of copper(ii) complexes of 4-(2-pyridylmethyl)-1,7-dimethyl-1,4,7-triazonane-2,6-dione and 4-(2-pyridylethyl)-1,7-dimethyl-1,4,7-triazonane-2,6-dione.four mononuclear copper(ii) complexes 1-4 have been synthesized with two new n-functionalized macrocyclic ligands l(1) and l(2). all complexes are well characterized by various spectroscopic techniques, elemental analyses and conductivity measurements. results suggest that cu(ii) ion has n(2)o coordination from ligand and s(2) from two coordinated solvent molecules (sch(3)cn for 1 and 3 while ch(3)oh for 2 and 4). the crystal structure of a representative complex 3 strengthen the proposed formul ...200918775587
presentation, treatment, and clinical course of otogenic lateral sinus thrombosis.otogenic lateral sinus thrombosis (olst) is almost always associated with other complications of chronic otitis media (com) and did not present a distinct clinical expression. in our experience, olst exhibits a benign course if the underlying disease is controlled. mastoidectomy in combination with broad-spectrum antibiotics provided effective treatment. anticoagulation therapy is no longer used routinely. recanalization is rare and is independent of the use of anticoagulants.200918781447
recombinant expression, purification, and biochemical characterization of chondroitinase abc ii from proteus vulgaris.chondroitin lyases (or chondroitinases) are a family of enzymes that depolymerize chondroitin sulfate (cs) and dermatan sulfate (ds) galactosaminoglycans, which have gained prominence as important players in central nervous system biology. two distinct chondroitinase abc enzymes, cabci and cabcii, were identified in proteus vulgaris. recently, cabci was cloned, recombinantly expressed, and extensively characterized structurally and biochemically. this study focuses on recombinant expression, pur ...200918849565
a survey on species and prevalence rate of bacterial agents isolated from cockroaches in three hospitals.the presence of cockroaches has health implications, such as nosocomial infection, as the insects move freely from areas within and around hospitals that may harbor pathogenic organisms. the goals of the present study were to determine species of bacteria isolated from cockroaches found in three tehran hospitals, and to determine distribution of potential vectors by species and sex. the study is descriptive laboratory research. a total of 305 cockroaches from three species were trapped and ident ...200918973441
characterization of a cryptic plasmid from an alpha-proteobacterial endosymbiont of amoeba proteus.a new cryptic plasmid pap3.9 was discovered in symbiotic alpha-proteobacteria present in the cytoplasm of amoeba proteus. the plasmid is 3869bp with a gc content of 34.66% and contains replication origins for both double-strand (dso) and single-strand (sso). it has three putative orfs encoding mob, rep and phosphoglycolate phosphatase (pgpase). the pap3.9 plasmid appears to propagate by the conjugative rolling-circle replication (rcr), since it contains all required factors such as rep, sso and ...200918951917
evaluation of screening methods to detect plasmid-mediated ampc in escherichia coli, klebsiella pneumoniae, and proteus mirabilis.there are currently no standardized phenotypic methods for the screening and detection of ampc enzymes. this study aimed to evaluate different methods to detect ampc enzymes in escherichia coli, klebsiella spp., and proteus spp., comparing the results from two disk-based methods and an agar dilution method. ampc activity was determined for 255 clinical isolates by use of a three-dimensional enzyme assay combined with a multiplex pcr assay for plasmid-borne ampc genes. these results were compared ...200918955528
utility of reflexive semen cultures for detecting bacterial infections in patients with infertility and leukocytospermia.this study evaluated the benefit of reflexive semen cultures in peroxidase-positive samples drawn from 17,142 consecutive samples analyzed, in which 4.7% (807/17,142) had leukocytospermia and 12.7% of those (103/807) grew significant bacterial colonies on culture. considering the potential ramifications of the use of samples with bacteriospermia, we conclude that reflexive culture results are cost-effective and prudent.200918980764
liquid chromatography-mass spectrometry to study chondroitin lyase action pattern.liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. two commercial enzymes, chondroitinase abc (proteus vulgaris) and chondroitinase acii (arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. next, the action patterns of recombinant lyases, chondroitinase abc from bacteroides thetaiotaomicron (expressed in escherichia coli) and chondroitinase ac from flav ...200918992215
development of recombinant escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from proteus sp. for biodiesel production.a lipase-producing bacterium k107 was isolated from soil samples of china and identified to be a strain of proteus sp. with genome-walking method, the open reading frame of lipase gene lipk107, encoding 287 amino acids, was cloned and expressed in a heterologous host, escherichia coli bl21 (de3). the recombinant lipase was purified and characterized, and the optimum ph of the purified lipk107 was 9, at 35 degrees c. the recombinant e. coli expressing lipk107 was applied in biodiesel production i ...200919007827
an explanatory model to validate the way water activity rules periodic terrace generation in proteus mirabilis swarm.this paper explains the biophysical principles which, according to us, govern the proteus mirabilis swarm phenomenon. then, this explanation is translated into a mathematical model, essentially based on partial differential equations. this model is then implemented using numerical methods of the finite volume type in order to make simulations. the simulations show most of the characteristics which are observed in situ and in particular the terrace generation.200919009295
chitosan acetate bandage as a topical antimicrobial dressing for infected burns.an engineered chitosan acetate bandage preparation (hemcon) is used as a hemostatic dressing, and its chemical structure suggests that it should also be antimicrobial. we previously showed that when a chitosan acetate bandage was applied to full-thickness excisional wounds in mice that had been infected with pathogenic bioluminescent bacteria (pseudomonas aeruginosa, proteus mirabilis, and staphylococcus aureus), it was able to rapidly kill the bacteria and save the mice from developing fatal in ...200919015341
characterization and sequence analysis of extended-spectrum-{beta}-lactamase-encoding genes from escherichia coli, klebsiella pneumoniae, and proteus mirabilis isolates collected during tigecycline phase 3 clinical trials.in concert with the development of novel beta-lactams and broad-spectrum cephalosporins, bacterially encoded beta-lactamases have evolved to accommodate the new agents. this study was designed to identify, at the sequence level, the genes responsible for the extended-spectrum-beta-lactamase (esbl) phenotypes of escherichia coli, klebsiella pneumoniae, and proteus mirabilis isolates collected during the global tigecycline phase 3 clinical trials. pcr assays were developed to identify and clone th ...200919015360
bacterial contamination in drinking water: a case study in rural areas of northern rajasthan, india.the objective of this study was to determine the bacterial contaminations in drinking water samples collected form some rural habitations of northern rajasthan, india. a total of ten bacterial species: escherichia coli, pseudomonas aeruginosa, enterobacter aerogenes, klebsiella sp, proteus vulgaris, alcaligenes faecalis, bacillus cereus, staphylococcus aureus, streptococcus lactis and micrococcus luteum were identified form drinking water samples. the bacteria belonging to the family enterobacte ...200919023670
resistances of antibiotics and heavy metals in enterobacteriaceae spp. isolated from gills and intestines of achanthobrama marmid (heckel,1843) from sir dam lake turkey.a total of 94 bacteria, associated with wild achanthobrama marmid (heckel, 1843) in sir dam lake of turkey identified. subsequently selected isolates were characterized and identified to the genus level the 94 members of enterobacteriaceae were isolated in the gills and intestines, and among the isolates, e. coli were represented at a rate of 55%, shigella spp. at a rate of 21%, salmonella spp. at a rate of 9%, citrobacter spp. at a rate of 9%, klebsiella spp. at a rate of 3% and proteus spp., a ...200920112859
[investigation of antibacterial activity of sertralin].sertralin is a psychotropic drug which acts by inhibiting the selective serotonin re-uptake in the synaptic area. previous studies have shown that some antidepressant agents have antibacterial activity. the aim of this study was to investigate the in vitro antibacterial activity of sertralin. a total of 224 bacterial strains isolated from clinical specimens together with standard control strains were included to the study. the antibacterial activity of sertralin was determined by microdilution m ...200920084919
antibacterial and antioxidant activities of the essential oil of artemisia echegarayi hieron. (asteraceae).artemisia echegarayi hieron. (asteraceae) is commonly known in argentina as "ajenjo". many studies report high efficacy of essential oils against food-borne pathogenic bacteria. the antimicrobial activity and minimal inhibitory concentration of a. echegarayi essential oil were evaluated against seven bacterial species of significant importance in food hygiene, by using the disc diffusion assay and the micro-well dilution method, respectively. volatile components of the extract were analyzed by g ...200920085186
[etiological factors of community acquired urinary tract infections in hospitalized patients].etiological factors of community-acquired urinary tract infections (uti) are specific for age, gender, season, complication of uti and type of uti. their prevalence and susceptibility to antimicrobial agents shows geographic and time variability.200920088156
antimicrobial resistance patterns of staphylococcus aureus and proteus spp. isolated from otitis media at bahir dar regional laboratory, north west ethiopia.otitis media is a major health problem of children in low income countries like ethiopia. thus, this study was aimed at determining the predominant bacterial isolates from discharging ear and their antimicrobial susceptibility patterns.200920067141
[antimicrobial treatment of nosocomial intra-abdominal infections--new treatment options with tygecycline].nosocomial, intra-abdominal infections are extremely serious conditions, considering possibilities for their early diagnosis, as well as for their effective therapy. multiresistant bacteria (enterobacteriacae producing extended-spectrum beta-lactamases - esbl escherichia coli, klebsiella species, vancomycin-resistant enterococci [vre], and methicillin-resistant staphylococcus aureus [mrsa]) are frequently isolated as pathogens of these infections. tygecycline is among the novel wide- spectrum an ...200920052927
intra/extracellular biosynthesis of silver nanoparticles by an autochthonous strain of proteus mirabilis isolated from photographic waste.there is an enormous interest in developing green synthesis procedures for production of nanoparticles by using biomimetic approaches. in our research focus has been given to the development of an efficient and eco-friendly viable process for the synthesis of nanoscale silver particles using proteus mirabilis ptcc 1710, a bacterial strain that was isolated during a screening program from photographic waste. a significant result of this study is our observation that silver nanoparticles could be ...200920055006
the influence of omentectomy on bacterial clearance: an experimental study.the influence of an omentectomy on peritoneal defense mechanisms and its clinical consequences have not been fully elucidated. in the present study, we aimed to investigate the influence of omentectomy on bacterial growth in blood and tissue specimen cultures in rats with experimental peritonitis.200920037870
effect of ibicella lutea on uropathogenic proteus mirabilis growth, virulence, and biofilm formation.proteus mirabilis, an important uropathogen that can cause complicated urinary tract infections (uti), has emerged as a therapeutic problem following mutations that compromise the use of antimicrobial drugs. due to the serious effects associated with uropathogenic p. mirabilis and the problems related to the use of antibiotics, it is necessary to develop alternative strategies for its control. the objective of this study was to assess the effect of ibicella lutea extract, a south american indige ...200920009277
[local control treatment of bacteria found present in an infected bed sore by means of a hydropolymer dressing with ionic silver].every chronic wound has microorganisms present on its surface and therefore, its contamination is universal and unavoidable, but the majority of these wounds are not infected. it is important, therefore, to refine the diagnosis of infection indentifying the microorganisms which have succeeded in gaining access to the submarginal tissues in order to be able to prevent unnecessary or inappropriate antibiotic treatment and to lessen possible complications. a high level of bacteria present, close to ...200920014620
nosocomial infections prevalence study in a serbian university hospital.nosocomial infections (ni) are a serious health problem resulting in an enromous burden of excess morbidity and mortaliti rates, and health care costs. the aim of this study was to assess the prevalence of ni and to identify groups of patients at special risk for ni in the university clinical center, kragujevac, serbia.200920017416
microbiological findings in prepubertal girls with vulvovaginitis.the aim of the study was to define the most common causes, symptoms and clinical features of vulvovaginitis in prepubertal girls, and to evaluate treatment success depending on the causative agent involved. the study included 115 girls aged 2-8 (mean 4.8) years, presenting with vulvovaginitis to the outpatient clinic for pediatric and adolescent gynecology, zagreb children's hospital, between september 2006 and july 2007. medical history data were obtained from parents. vaginal samples were coll ...200920021979
bacteriological evaluation of pre-cut fruits sold in kano metropolis, kano state, nigeria.one hundred and fifty (150) pre-cut fruit samples comprising of pineapples (50), paw-paw (50) and watermelon (50) at the point of stand retail outlets were tested by standard bacteriological methods to determine bacterial contamination of the fruits. out of these 150 examined 136 (90.67%) were contaminated with bacteria. the bacterial distribution were; escherichia coli 69 (46.00%), staphylococcus aureus 29 (19.33%), salmonella species 13 (8.67%), proteus species 18 (12.00%), enterobacter aeroge ...200920000065
cross-reaction between proteins of larrea divaricata cav. (jarilla) and proteins of gram-negative bacteria.larrea divaricata is an abundant plant of northwest of argentina used to treat different pathologies. we aimed to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (jpce) of jarilla. we evaluated the cross reaction between jpce and whole cell-bacterial proteins (w-cbp) of escherichia coli, pseudomonas aeruginosa, proteus vulgaris, and klebsiella pneumoniae using a mouse anti-jpce serum. protein profiles of jpce and w-cbp were analyzed. for jpce, 18 bands ...200919874237
hospital and community isolates of uropathogens at a tertiary hospital in south africa.to investigate the profile of common uropathogens isolated from urine specimens submitted to the diagnostic microbiology laboratory at a tertiary teaching hospital and assess their antimicrobial susceptibility patterns to commonly used antimicrobial agents.200919908617
infections and treatment of wounds in survivors of the 2004 tsunami in thailand.on 26 december 2004, a tsunami devastated the west coast of thailand and caused 8457 injuries and 5395 deaths. data were collected from 26 december 2004 to 31 january 2005 at four public hospitals to describe the character and treatment of wounds of 523 persons who were injured during tsunami and sought medical treatment. wounds were contaminated with mud, sand, debris and sea water and had an infection rate of 66.5% (674/1013). most wounds (45%) had poly-microbial infection with gram-negative r ...200919912391
identification and characterization of the metal ion-dependent l-alanoyl-d-glutamate peptidase encoded by bacteriophage t5.although bacteriophage t5 is known to have lytic proteins for cell wall hydrolysis and phage progeny escape, their activities are still unknown. this is the first report on the cloning, expression and biochemical characterization of a bacteriophage t5 lytic hydrolase. the endolysin-encoding lys gene of virulent coliphage t5 was cloned in escherichia coli cells, and an electrophoretically homogeneous product of this gene was obtained with a high yield (78% of total activity). the protein purified ...200919919545
chronic suppurative otitis media in cleft palate: microorganism etiology and susceptibilities.to investigate the microbial etiology of suppurative chronic otitis media (scom) in patients with complete cleft lip and palate and isolated cleft palate and to determine the sensitivity of isolated microorganisms to antibiotics by drug diffusion from impregnated discs in agar and the minimum inhibitory concentration of each drug to these microorganisms by drug dilution in agar.200919929097
quantitative imaging of cellular adhesion by total internal reflection holographic microscopy.total internal reflection (tir) holographic microscopy uses a prism in tir as a near-field imager to perform quantitative phase microscopy of cell-substrate interfaces. the presence of microscopic organisms, cell-substrate interfaces, adhesions, and tissue structures on the prism's tir face causes relative index of refraction and frustrated tir to modulate the object beam's evanescent wave phase front. we present quantitative phase images of test specimens such as amoeba proteus and cells such a ...200919956284
antimicrobial properties of the stem bark of saraca indica (caesalpiniaceae).chloroform, methanol, aqueous and ethanolic extracts of the stem bark of saraca indica were investigated for their antibacterial and antifungal activity against standard strains of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus cereus, klebsiella pneumoniae, proteus mirabilis, salmonella typhimurium and streptococcus pneumoniae and the fungi: candida albicans and cryptococcus albidus. methanolic and aqueous extract exhibited antimicrobial activity with mic ranging from ...200919961043
electrostatic binding of substituted metal phthalocyanines to enterobacterial cells: its role in photodynamic inactivation.the effect of ionic substituents in zinc and aluminum phthalocyanine molecules and of membrane surface charge on the interaction of dyes with artificial membranes and enterobacterial cells, as well as on photosensitization efficiency was studied. it has been shown that increasing the number of positively charged substituents enhances the extent of phthalocyanine binding to escherichia coli cells. this, along with the high quantum yield of singlet oxygen generation, determines efficient photodyna ...200919961410
[enteropathogens relating to diarrhea in hiv patients on antiretroviral therapy].the etiology of the diarrheic process in aids may be caused by viruses, bacteria, fungi, protozoa or helminths, as well as hiv itself. this study evaluated enteropathogens relating to diarrhea in hiv patients who were on antiretroviral therapy. the parasitological methods used were faust, hoffmann and kinyoun. isolation and culturing of fungi were carried out in accordance with the methodology recommended by the nccls m27-a standard. the yeast species were identified using the polymerase chain r ...200919967238
tensile strength of absorbable suture materials: in vitro analysis of the effects of ph and bacteria.to evaluate the tensile strength and suture degradation of 5 commonly used absorbable suture materials in different ph and bacteria inoculated media.200919896625
joint extraintestinal manifestations in ulcerative colitis.an extraintestinal manifestation (eim) very often occurs in ulcerative colitis (uc) patients. eim modifies the natural course of uc and decreases the quality of life in these patients. the aim of this study was to analyze clinical and laboratory findings in uc patients with joint eim. 319 uc patients were examined. among them were 131 (41.1%) patients with distal uc, 102 (32.0%) suffered from left-sided uc and 86 (26.9%) had pancolitis. 95 (29.8%) uc patients had joint eim. arthritis correlated ...200919897966
effect of ciprofloxacin and n-acetylcysteine on bacterial adherence and biofilm formation on ureteral stent surfaces.the aim of this study was to evaluate the effect of ciprofloxacin (cip), n-acetylcysteine (nac) alone and in combination on biofilm production and pre-formed mature biofilms on ureteral stent surfaces. two strains each of staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebseilla pneumoniae, pseudomonas aeruginosa and proteus vulgaris, recently isolated from patients undergoing ureteral stent removal and shown to be capable of biofilm production, were used in this study. the ...200919899620
the cross-reactivity of shewanella fidelis lipopolysaccharide with anti-proteus antibodies.the serological cross-reactivity between lipopolysaccharides (lps) of s. fidelis kmm3582(t) and rabbit anti-o p. mirabilis antibodies was tested. using elisa and western blot cross-reactivity between s. fidelis lps and antisera against p. mirabilis o14, o3 lpss was found. the observed cross-reaction may suggest that anti-p. mirabilis s1959 (o3) antibodies may bind to the internal part of s. fidelis o-polysaccharides. a weak interaction between s. fidelis lps and antiserum against p. mirabilis o1 ...200919899622
comparison of 3 phenotypic-detection methods for identifying plasmid-mediated ampc beta-lactamase-producing escherichia coli, klebsiella pneumoniae, and proteus mirabilis strains.plasmid-mediated ampc beta-lactamases (pabls) have been detected in the strains of escherichia coli, klebsiella spp., proteus mirabilis, and salmonella spp. pabls may be difficult to detect and might interfere in the therapeutic and infection-control processes. although several pabl-detection methods based on phenotypes have been reported, the clinical and laboratory standards institute currently does not recommend a routine detection method for pabls. the aim of this study is to compare the per ...200919893354
assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance.to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health.200919880150
risk factors for hospital-acquired pneumonia in nonventilated adults.although most recent publications focus on ventilator-associated pneumonia, non-ventilator-associated hospital-acquired pneumonia (nvhap) is still worrisome. we studied risk factors for nvhap among patients admitted to a small teaching hospital. sixty-six nvhap case patients and 66 controls admitted to the hospital from november 2005 through november 2006 were enrolled in a case-control study. variables under investigation included: demographic characteristics, comorbidities, procedures, invasiv ...200920231992
notes on the microbiology of cholesteatoma: clinical findings and treatment.anomalous proliferation of the cholesteatoma epithelium is caused by extrinsic factors such as toxins or bacterial antigens combined with lytic enzymes, lymphokines and cytokines released from the inflammatory infiltrate. this could explain the close relationship between the aggressiveness of cholesteatoma and repeated bacterial superinfection, therefore it is very important to know the bacteria involved in order to control the regrowth of skin following surgery, reduce the aggressive potential ...200920161877
[isolation and characterization of shewanella spp. from patients of food poisoning].to identify the isolates of shewanella spp. from specimens of food poisoning based on biological and biochemical analysis.200920193210
[susceptibility of enterobacteriaceae rods to selected tetracyclines].the aim of this study was to evaluated the susceptibility of enterobacteriaceae rods to tigecycline, tetracycline and doxycycline. we analysed 121 strains belonged to species as follows: escherichia coli (40 strains), proteus mirabilis (22), enterobacter cloacae (19), klebsiellapneumoniae (18), morganella morgannii (13), serratia marcescens (4), klebsiella oxytoca (3) i proteus vulgaris (2). all strains of e. coli, k. oxytoca, s. marcescens and p. vulgaris were susceptible to tigecycline. twenty ...200920201321
microbial evaluation and deterioration of paints and paint-products.the microbial quality of materials and final products of a reputable paint industry in lagos area were analysed. the bacterial contaminants isolated in the paint-products included bacillus brevis, b. polymyxa, b. laterosporus, lactobacillus gasseri, l. brevis, escherichia coli and proteus mirabilis. the fungal contaminants detected in the paints were mainly aspergillus niger, a. flavus and penicillium citrinum. the microbial populations in the raw materials ranged from 1.0 x 10(6) - 9.5 x 10(6) ...200920143715
pathogen incidence and antibiotic resistance patterns of catheter-associated urinary tract infection in children.the aim of this study was to characterize the pathogens and their antibiotic susceptibilities in children with catheter-associated urinary tract infection (cauti) in order to optimize empirical antibiotic therapy and prophylaxis. from 2001 to 2006, 895 children with an indwelling catheter from 3 hospitals in china were included in this study, of whom 335 (37.4%) had cauti. antimicrobial susceptibility testing of 450 bacterial isolates was performed using the clsi broth and kirby-bauer agar dilut ...200920071290
use of the cefepime-clavulanate esbl etest for detection of extended-spectrum beta-lactamases in ampc co-producing bacteria.extended-spectrum beta-lactamases (esbls) may not always be detected in routine susceptibility tests. this study reports the performance of the cefepime-clavulanate esbl etest for the detection of esbls in enterobacteriaceae, including those producing ampc enzyme.200920130375
phytochemical and antimicrobial activity of swietenia mahagoni crude methanolic seed extract.the present study was designed to evaluate the antibacterial activities of swietenia mahagoni crude methanolic (smcm) seed extract. the antimicrobial activity of the oily extract against gram-positive, gram-negative, yeast and fungus strains was evaluated based on the inhibition zone using disc diffusion assay, minimal inhibition concentration (mic) and minimal bactericidal concentration (mbc) values. the crude extract was subjected to various phytochemicals analysis. the demonstrated qualitativ ...200920237441
extended spectrum beta-lactamase detection in gram-negative bacilli of nosocomial origin.resistance to third generation cephalosporins by acquisition and expression of extended spectrum beta lactamase (esbl) enzymes among gram-negative bacilli is on a rise. the presence of esbl producing organisms significantly affects the course and outcome of an infection and poses a challenge to infection management worldwide.200920300397
proteus vertebral osteomyelitis.proteus organisms are rare causes of osteomyelitis. vertebral osteomyelitis caused by this organism has only been reported in 21 cases over the last 75 years. although staphylococcus aureus is the most common cause of vertebral osteomyelitis, rare causes such as p. mirabilis may need to be considered in the context of recent urinary tract infection or urological surgery. therefore biopsy should be undertaken to establish the causative organism and determine appropriate antibiotic susceptibility.200920374334
increasing resistance to quinolones: a four-year prospective study of urinary tract infection pathogens.a four-year prospective study was carried out to determine the incidence and rate of development of resistance by common urinary tract infection (uti) pathogens to quinolone antimicrobial agents. results show that there is high intrinsic resistance to the quinolones among strains of pseudomonas aeruginosa (43.4%), escherichia coli (26.3%), and proteus spp. (17.1%). over four years, rising rates of resistance were observed in p. aeruginosa (14.6% increase), staphylococcus aureus (9.8%), and e. co ...200920360901
[etiological structure and sensitivity to antibiotics of the infection process pathogens in general surgical hospital].the studying of the species and their sensitivity to antibiotics of mostly spread infection factors constitute one of the main trends in intrastationary infection prophylaxis. in 307 patients, suffering the infection processes of various localization, such as purulent wounds, cholecystitis, cholangitis, peritonitis etc., there were studied up the species and sensitivity to antibiotics of the microorganisms determined. staphylococcus spp. and e. coli were determined most frequently, somewhat rare ...200920458947
screening of antibacterial potentials of some medicinal plants from melghat forest in india.cyperus rotundus, caesalpinia bonducella, tinospora cordifolia, gardenia gummifera, ailanthus excelsa, acacia arabica, embelia ribes and ventilago maderspatana from melghat forest were screened for their antibacterial potential against escherichia coli, staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris, salmonella typhi, shigella flexneri, salmonella paratyphi, salmonella typhimurium, pseudomonas aeruginosa, enterobacter aerogenes by disc diffusion method. out of these medicinal pla ...200920448847
a survey of veterinary hospitals in nigeria for the presence of some bacterial organisms of nosocomial and zoonotic potential.this study was undertaken to determine the type and estimate the prevalence of bacterial organisms on contact surfaces of five close-to-patient facilities in three veterinary health care settings within the sokoto metropolis of north-western nigeria. a total of 30 samples (10 from each setting) were collected and analysed using culture, microscopy and biochemical testing. bacterial species isolated from samples in this study included the following: bacillus sp. (27.3%), staphylococcus aureus (15 ...200920391375
potential airborne microbial hazards for workers on dairy and beef cattle farms in egypt.this study was conducted to determine the concentration and frequency distribution of certain airborne micro-organisms on cattle farms and their potential health hazards to farm workers. the samples (60 air samples and 240 hand and nasal swabs from cattle farm workers) were collected from ten cattle farms (five dairy barns and five beef sheds) located in the sharkia governorate of egypt. air samples were collected for microbiological examination in liquid media using an all-glass impinger wherea ...200920391378
bioefficacy of lantana camara l. against some human pathogens.antimicrobial efficacy of flavonoids (free and bound) and crude alkaloids of lantana camara l. was determined by disc diffusion assay against three bacteria (escherichia coli, proteus mirabilis, and staphylococcus aureus) and two fungi (candida albicans and trichophyton mentagrophytes). minimum inhibitory concentration, minimum bactericidal/fungicidal concentration and total activity were also studied. most susceptible microorganism in the present study was c. albicans followed by p. mirabilis, ...200920502587
antibiotic resistance of gram negative bacilli strains isolated from the intensive care unit in fundeni clinical institute, bucharest, romania.the aim of this study was to investigate the antibiotic resistance profile of 58 gram negative bacilli strains (gnb): 36 non-fermentative gnb (ngnb), including 19 strains of acinetobacter spp., 11 of pseudomonas aeruginosa, 6 of stenotrophomonas maltophilia and 22 enterobacterial strains (14 strains of kehss, 6 belonging to the group proteus-providencia and 2 escherichia coli) isolated from nasal, pharyngeal exudates and also from bronchial secretions, from immuno-depressed patients admitted in ...200920583477
[clinical assessment of novel chromid esbl agar plates for detection of esbl producers in the family enterobacteriaceae].extended-spectrum beta-lactamase (esbl)-producers in the family enterobacteriaceae are recognized worldwide as nosocomial pathogens, however it is difficult to screen them in the routine laboratory processing. chromid esbl agar newly developed for screening esbl-producing enterobacteriaceae was released in japan in april, 2007. we evaluated the clinical assessment of chromid esbl agar in routine microbiology laboratory. the 47 strains investigated were clinical isolates belonging to the family e ...200920528091
[etiologic factors in urinary tract infection investigated in ambulatory setting. community-acquired urinary tract infection: etiologic aspects and antibiotic resistance].urine bacteriological examination is one of the most frequently asked for examinations, in ambulatory as well as in hospitals, regarding specialty prophyle. the present study comprises a group of 3971 pacients that were sent to the laboratory for uroculture and showed a percentage of only 21.85% significant urocultures--the etiological groups most frequently isolated being gram-negative bacilli--96.05%, respectively escherichia sp. 76.95%, klebsiella sp. 14.68%, proteus sp. 4.66%. out of the gra ...200920524391
prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae in bahrain.to determine the occurrence of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in bahrain.200920701872
the in vitro antimicrobial activity of fruit and leaf crude extracts of momordica charantia: a tanzania medicinal plant.to evaluate the antimicrobial activity of momordica charantia extracts on reference strains and microorganisms isolated from clinical specimens.200920842240
effect of keratin-gelatin and bfgf-gelatin composite film as a sandwich layer for full-thickness skin mesh graft in experimental dogs.skin grafts are indicated when there is a major loss of skin. full-thickness skin graft is an ideal choice to reconstruct defect of irregular surface that is difficult to immobilize. full-thickness mesh grafts can be applied to patch large skin defect when there is less donor site in extensively traumatized and burned surgical patients. the concept of using natural biomaterials such as keratin, basic fibroblast growth factor is slowly gaining popularity in the field of medical research to achiev ...200918161832
[nosocomial urinary tract infections in the geriatric hospital - pathogen spectrum and resistancies].we examined 834 isolates of urinary cultures (26 fungi and 808 isolates from 28 bacteria) in an academic geriatric teaching hospital for microbial spectrum and resistance analysis. of the bacteria, 90% were associated to ten species. detection rate of mrsa and esbl was 1%, detection rate for pseudomonas aeruginosa, proteus species, coagulase negative staphylococci and klebsiella species were higher than in an university clinic (p < 0.001). resistency rates were high for the 10 most frequent bact ...200918327687
identification of plasmid-encoded extended spectrum beta-lactamases produced by a clinical strain of proteus mirabilis.emergence and dissemination of multiresistant strain of proteus mirabilis have made infections treatment more difficult that this bacterium is responsible. the aim of this study is to determine the implication of the enzymatic mechanism and to describe the properties of esbls (extended spectrum beta-lactamases). a clinical strain of proteus mirabilis sm514 isolated in the intensive care unit at the military hospital in tunisia during the period 2004 was found to be highly resistant to cephalospo ...200918456422
antibacterial iridoid glucosides from eremostachys laciniata.eremostachys laciniata (l) bunge (family: lamiaceae alt. labiatae; subfamily: lamioideae) is one of the 15 endemic iranian herbs of the genus eremostachys. a decoction of the roots and flowers of e. laciniata has traditionally been taken orally for the treatment of allergies, headache and liver diseases. three antibacterial iridoid glucosides, phloyoside i (1), phlomiol (2) and pulchelloside i (3) have been isolated from the rhizomes of this plant. the structures of these compounds were elucidat ...200918693303
a novel biosorbent for dye removal: extracellular polymeric substance (eps) of proteus mirabilis tj-1.this paper deals with the extracellular polymeric substance (eps) of proteus mirabilis tj-1 used as a novel biosorbent to remove dye from aqueous solution in batch systems. as a widely used and hazardous dye, basic blue 54 (bb54) was chosen as the model dye to examine the adsorption performance of the eps. the effects of ph, initial dye concentration, contact time and temperature on the sorption of bb54 to the eps were examined. at various initial dye concentrations (50-400 mg/l), the batch sorp ...200918718709
regioselective synthesis and antimicrobial screening of novel ketocarbazolodispiropyrrolidine derivatives.a series of novel dispiropyrrolidine derivatives have been synthesized through 1,3-dipolar cycloaddition reaction of azomethine ylide generated from sarcosin and di/tri ketone with the dipolarophile (e)-2-arylidine-1-keto-carbazoles. the cycloadducts ketocarbazalo spiro n-methyl pyrrolidines showed the most interesting antimicrobial activity at lower concentration.200918722036
lipase-catalyzed production of a bioactive fatty amide derivative of 7,10-dihydroxy-8(e)-octadecenoic acid.enzymatic syntheses of fatty amides are of considerable interest due to their wide ranging industrial applications in detergents, shampoo, cosmetics and surfactant formulations. amidation reaction of candida antarctica lipase b (calb) was investigated for direct amidation of carboxylic acid in organic solvent. calb-mediated production of a novel secondary amide was carried out by reacting the hydroxy oleic acid derivative, 7,10-dihydroxy-8(e)-octadecenoic acid (dod), with n-methylethanol amine i ...200918793843
synthesis and biological activities of new hydrazide derivatives.the synthesis of a new series of imidazo[1,2-a]pyrazine-2-carboxylic acid arylidene-hydrazides is described. the chemical structures of the compounds were elucidated by ir, (1)h-nmr, fab(+)-ms spectral data. their biological activity against various bacteria, fungi species, and mycobacterium tuberculosis was investigated. antibacterial activity was measured against escherichia coli (nrrl b-3704), staphylococcus aureus (nrrl b-767), salmonella typhimurium (nrrl b-4420), proteus vulgaris (nrll b-1 ...200918825530
causative agents of urinary tract infections in children and their antibiotic sensitivity pattern: a hospital based study.a retrospective study was conducted to find out the causative agents of urinary tract infection (uti) in children and their antibiotic sensitivity pattern among nepalese children. this was done at kanti children's hospital in kathmandu (nepal) by analyzing the records of urine samples collected for culture and sensitivity tests over a period of six months (april to november, 2007). of the total 1878 mid-stream urine samples collected from suspected cases of uti, 538 (28.6%) were positive for pat ...200818828428
distribution frequency of pathogenic bacteria isolated from cutaneus leishmaniasis lesions.cutaneous leishmaniasis (cl) is a parasitic disease characterized by single or multiple ulcerations. secondary bacterial infections are one of the complications that can increase the tissue destruction and the resulting scar. to better determine the incidence of real secondary bacterial infections in cl, we designed the current study. this was a cross-sectional study performed in skin diseases and leishmaniasis research centre, isfahan, iran. a total of 1,255 patients with confirmed cl enrolled ...200818830062
[the analysis of the bacterial and fungal flora in maxillary sinuses in patients operated due to fess method].the aim of this work was to analyse the bacterial and fungal flora in maxillary sinuses in patients operated due to the fess method.200818837224
[macrodactylies of the hand and foot].macrodactyly is a rare congenital anomaly defined by an enlargement of all the structures of fingers or toes. two forms of macrodactyly have been described. one form is static with an enlargement present at birth and growing proportionately to the other digits. the other is progressive with an enlargement beginning in early childhood and growing faster than that of the normal digits. macrodactyly is responsible of a functional handicap and an aesthetic prejudice. treatment may be indicated for t ...200818842439
uropathogens and host characteristics.uropathogens other than escherichia coli occur with greater frequency in patients with risk factors for urinary tract infection (uti). however, little is known about associations between uropathogen species and host characteristics. three hundred nineteen urine specimens containing a balanced distribution of uropathogen species were selected from inpatients and outpatients at a university hospital clinical microbiology laboratory. information on host characteristics was retrospectively collected ...200818842936
[fluoroquinolones and gram-negative bacteria: antimicrobial activity and mechanisms of resistance].fluoroquinolones acts by interacting with type ii topoisomerases (dna gyrase and topoisomerases iv). related to this mechanism of action, bacteria have developed resistance mechanisms consisting in some target mutations (gyra/gyrb for dna gyrase and parc/pare for topoisomerase iv) or in a reduced access to the target itself, by either decreased permeability or augmented expression of efflux pumps, such as acrab and mexab. along with these classical mechanisms of chromosomal resistance, the prese ...200818843219
experimental and qsar of acetophenones as antibacterial agents.antibacterial activity of 20 acetophenone derivatives was evaluated against two gram-positive and three gram-negative organisms, namely, bacillus subtilis ncim 2718, staphylococcus aureus ncim5021, salmonella typhi ncim2501, enterobacter aerogenes ncim5139, and proteus vulgaris ncim2813 by two-fold dilution method. the most active amongst this group of compounds were 4-methyl, 2-hydroxy, 3-bromo, 4-ethoxy, 3-nitro and 4-nitro acetophenones. quantitative structure-activity relationships were deve ...200818844677
[recent evolution and characterization of extended-spectrum beta-lactamase producing enterobacteria in the chu of nice (2005-2007)].esbl producing enterobacteria (e-esbl+) are always a public health concern, mainly due to increase of ctx-m beta-lactamase. so, 542 new strains were isolated in the chu de nice during the 2005-2007 period. the aim of this work was to characterize the esbl and to type isolates suspected to be implicated in outbreaks.200818848404
genetic and phenotypic heterogeneity in the pten hamartoma tumour syndrome.germline pten (phosphatase and tensin homologue deleted on chromosome ten) mutations predispose to phenotypically diverse disorders that share several overlapping clinical features: cowden syndrome, bannayan-riley-ruvalcaba syndrome, proteus syndrome and proteus-like syndrome, collectively classified as pten hamartoma tumour syndrome (phts). the meticulous acquisition and documentation of phts phenotypic data at different levels and the profiling of the plethora of genetic changes in pten and ot ...200818794875
congenital lipomatous overgrowth, vascular malformations, and epidermal nevi (clove) syndrome: cns malformations and seizures may be a component of this disorder.a newborn girl was found to have a massive lymphatic truncal vascular malformation with overlying cutaneous venous anomaly associated with overgrown feet and splayed toes. these manifestations comprise the recently described clove syndrome. she also had cranial asymmetry and developed generalized seizures, which were treated with anticonvulsants. cranial ct showed encephalomalacia, widening of the ventricles and the sulci, hemimegalencephaly (predominantly white matter) and partial agenesis of c ...200818816642
molecular identification of streptococcus equi subsp. equi and streptococcus equi subsp. zooepidemicus in nasal swabs samples from horses suffering respiratory infections in iran.the objective of this study was to evaluate the existence of streptococcus equi subsp. equi and streptococcus equi subsp. zooepidemicus as probable agents associated with naturally occurring infection of the equine upper respiratory disease in mashhad area. nasal swabs samples from thirty horses with upper respiratory tract infections were collected. the bacteria isolated and identified were streptococcus equi subsp. equi (1 isolate), streptococcus equi subsp. zooepidemicus (25 isolates), pasteu ...200818817175
inhibitory effects of sage extract on the growth of enteric bacteria.antibacterial activity of sage extract at concentrations of 0.1, 0.05, 0.025, 0.0125, 0.00625, 0.003125, 0.00156, 0.0005 and 0.00025 g dl(-1) against salmonella typhi, shigella sonnei, s. flexneri, proteus vulgaris, staphylococcus aureus, etec escherichia coli and pseudomonas aeruginosa was evaluated. susceptibility testing of bacterial strains against 18 antibiotics was also performed for comparison. the results showed that p. aeruginosa and etec e. coli were completely resistant to sage extrac ...200818817180
antibacterial activity of the fruits of iranian torilis leptophylla against some clinical pathogens.the aim of this study was to examine the antimicrobial activity of the methanolic extract of torilis leptophylla was tested on eleven bacteria (bacillus anthracis, bacillus subtilis, bacillus pumilus, staphylococcus aureus, bacillus licheniformis, brucella melitensis, escherichia coli, salmonella typhi, proteus mirabilis, bordetella bronshiseptica and pseudomonas aeruginosa). tested extract was effective against all bacteria but not b. subtilis. consequently, the ethanolic extract had antibacter ...200818819541
non-susceptibility trends among enterobacteriaceae from bacteraemias in the uk and ireland, 2001-06.enterobacteriaceae are common agents of bacteraemia, with escherichia coli accounting for 20% of the cases. reflecting this importance, members of the family constitute 4 of the 12 collection groups in the british society for antimicrobial chemotherapy (bsac) bacteraemia surveillance programme.200818819979
inhibition of candida albicans isocitrate lyase activity by sesterterpene sulfates from the tropical sponge dysidea sp.seven sesterterpene sulfates (1-7) were isolated from the tropical sponge dysidea sp. and their inhibitory activities against isocitrate lyase (icl) from candida albicans were evaluated. among the isolated natural products compound 6 and 7 were found to be strong icl inhibitors. the isolated compounds (1-7) also showed potent antibacterial effect against bacillus subtilis and proteus vulgaris, but did not display antifungal activity.200818824352
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