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clove syndrome with nevus unis lateris: report of a case.congenital lipomatous overgrowth, vascular malformations, and epidermal nevi (clove) syndrome is a recently described syndrome similar to proteus syndrome but lacking the progressive or distorting bony overgrowth of proteus syndrome. we describe a neonate with features of clove syndrome and nevus unius lateris.201020609160
rapidly spreading ctx-m-type beta-lactamase-producing proteus mirabilis in japan.in recent years, increased isolation of extended-spectrum beta-lactamase (esbl)-producing proteus mirabilis has been reported in japan. we undertook an investigation to determine the prevalence of esbl-producing p. mirabilis isolated in japan and to characterise the genotype. seventy-four p. mirabilis isolates recovered from specimens at 54 hospitals in japan between march and october 2006 were included in the study. of the 74 p. mirabilis isolates examined, 28 (37.8%) were esbl-producers. the b ...201020609568
laboratory surveillance for prospective plasmid-mediated ampc beta-lactamases in the kinki region of japan.extended-spectrum beta-lactamases, plasmid-mediated ampc beta-lactamases (pabls), and plasmid-mediated metallo-beta-lactamases confer resistance to many beta-lactams. in japan, although several reports exist on the prevalence of extended-spectrum beta-lactamases and metallo-beta-lactamases, the prevalence and characteristics of pabls remain unknown. to investigate the production of pabls, a total of 22,869 strains of 4 enterobacterial species, escherichia coli, klebsiella pneumoniae, klebsiella ...201020610688
chemical composition and biological activities of santiria trimera (burseraceae) essential oils from gabon.water-distilled essential oils from leaves and bark of santiria trimera (oliv.) aubrév. (burseraceae) collected in gabon were analyzed using capillary gc-fid and gc-ms. the leaf essential oil, which is described for the first time, was dominated by sesquiterpenes (76.5%), among which alpha-humulene (34.6%) and beta-caryophyllene (14.9%) were the major components. the bark essential oil was almost exclusively monoterpenic, with alpha-pinene (51.5%) and alpha-terpineol (16.8%) as main constituents ...201020614835
isoprenoid metabolism as a therapeutic target in gram-negative pathogens.gram-negative enterobacteria include a variety of human pathogens, perhaps most notably e. coli, salmonella, shigella, yersinia, and proteus. while there are treatment options for the diseases caused by these organisms, multi-drug resistance is often a problem and development of novel antibiotics has lagged over recent years. in humans, the isoprenoid biosynthetic pathway has become a subject of intense research for therapeutic modulation of human enzymes in diseases including hypercholesterolem ...201020615187
functional identification of the proteus mirabilis core lipopolysaccharide biosynthesis genes.in this study, we report the identification of genes required for the biosynthesis of the core lipopolysaccharides (lpss) of two strains of proteus mirabilis. since p. mirabilis and klebsiella pneumoniae share a core lps carbohydrate backbone extending up to the second outer-core residue, the functions of the common p. mirabilis genes was elucidated by genetic complementation studies using well-defined mutants of k. pneumoniae. the functions of strain-specific outer-core genes were identified by ...201020622068
rapid paper disk test for identification of helicobacter pylori in mixed cultures of gerbil gastric homogenates.a method denominated rapid paper disk test (rpdt) was developed to identify h. pylori colonies in complex cultures obtained from gerbil gastric homogenates. identification is based on a characteristic reaction pattern (rp) for h. pylori colonies given by the combination of the urease-oxidase activities on a paper disk. compared to the rps obtained from gerbil's intestinal tract isolated bacteria, h. pylori rp is completely distinguishable, even from those of bacteria that share one or both activ ...201020624429
history of polish neurology and neurosurgery. brudziński, jósef . 201020625969
the use of lytic bacteriophages in the prevention and eradication of biofilms of proteus mirabilis and escherichia coli.antibiotics have been the cornerstone of the clinical management of bacterial infections since their discovery in the early part of the last century. eight decades later, their widespread, often indiscriminate use, has resulted in an overall reduction in their effectiveness, with reports of multidrug-resistant bacteria now commonplace. increasing reliance on indwelling medical devices, which are inherently susceptible to biofilm-mediated infections, has contributed to unacceptably high rates of ...201020528927
the group of epidermal nevus syndromes part i. well defined phenotypes.the epidermal nevus syndromes represent a group of distinct disorders that can be distinguished by the type of associated epidermal nevus and by the criterion of presence or absence of heritability. well defined syndromes characterized by organoid epidermal nevi include schimmelpenning syndrome, phacomatosis pigmentokeratotica, nevus comedonicus syndrome, angora hair nevus syndrome, and becker nevus syndrome. the molecular basis of these disorders has so far not been identified. by contrast, the ...201020542174
the group of epidermal nevus syndromes part ii. less well defined phenotypes.in addition to the well established epidermal nevus syndromes, such as schimmelpenning, becker, proteus, child, or nevus comedonicus syndromes, the group of less well defined phenotypes associated with epidermal nevi presently includes nevus trichilemmocysticus syndrome, didymosis aplasticosebacea, scalp syndrome (sebaceous nevus, central nervous system malformations, aplasia cutis congenita, limbal dermoid,and pigmented nevus), gobello syndrome, bäfverstedt syndrome, nevada syndrome (nevus epid ...201020542175
comparative in vitro activity of cefditoren and other antimicrobials against enterobacteriaceae causing community-acquired uncomplicated urinary tract infections in women: a spanish nationwide multicenter study.cefditoren is a third-generation orally administered cephalosporin with a broad spectrum of activity against gram-positive and gram-negative bacterial species. after an oral 400-mg single dose, the mean concentrations in urine are 186.5 mg/l at 2 to 4 h and 12.7 mg/l at 8 to 12 h, and it is a potential drug to be used in the treatment of urinary tract infection (uti). we performed a multicenter nationwide study in spain in order to determine the in vitro activity of cefditoren and other comparat ...201020542206
[detection of bla(ctx-m) beta-lactamase genes in extended-spectrum beta-lactamase producing gram-negative bacteria].widespread production of ctx-m type extended-spectrum beta-lactamases (esbl) in enterobacteriaceae strains which are resistant to extended-spectrum cephalosporins is the most remarkable example for rapid and global spread of plasmid mediated antimicrobial resistance in bacteria. consecutive 200 esbl producing enterobacteriaceae strains out of 1640 isolates that were obtained from clinical samples (167 urine, 11 wound, 7 bronchoalveolar lavage, 3 peritoneal fluid, 2 cerebrospinal fluid, 2 biopsy, ...201020549952
emergence of proteus mirabilis isolates producing tem-52 extended-spectrum beta-lactamases in croatia.an increased frequency of extended-spectrum beta-lactamase (esbl)-positive proteus mirabilis isolates was observed recently in the clinical hospital center split in croatia. the aim of this study was the molecular characterization of esbls in p. mirabilis isolates from this hospital.201020551637
a genome-wide analysis of loss of heterozygosity and chromosomal copy number variation in proteus syndrome using high-density snp microarrays.excessive cell proliferation and genetic changes such as loss of an allele (loss of heterozygosity (loh)) or amplifications or deletions of parts of chromosomes (copy number variations (cnv)) are common findings in cancers. it is unknown whether these changes are also present in patients with overgrowth syndromes, although the presence of small-scale cnvs (such as duplication of 11p15 in beckwith-wiedemann syndrome), excessive cell proliferation and an increased frequency of tumors have all been ...201020555334
determinants of beta-lactam resistance in meningitis-causing enterobacteriaceae in brazil.this study analyzed resistance determinants in extended-spectrum beta-lactamase (esbl)-producing enterobacteria and the epidemiology of 11 escherichia coli isolates obtained from meningitis patients in a region of brazil from 2000 to 2005. esbl-encoding genes and their genetic environment were investigated by pcr and sequencing. the gene blactx-m-2 was identified in 3 different enterobacteria (e. coli, serratia marcescens, and proteus mirabilis) downstream of the insertion sequence iscr1 (locali ...201020555402
synthesis, antibacterial activity evaluation and qsar studies of novel dispiropyrrolidines.a series of novel dispiropyrrolidines have been synthesized through 1,3-dipolar cycloaddition of an azomethine ylide generated from sarcosine and isatin with the dipolarophile 3-benzylidene-1-methyl-pyrrolidine-2,5-dione. their antibacterial activity was evaluated against bacillus subtilis ncim 2718, staphylococcus aureus ncim5021, salmonella typhi ncim2501, pseudomonas aeruginosa ncim 5029 and proteus vulgaris ncim2813 by two fold dilution method. compound 6e exhibits reasonably good activity a ...201020556891
generation of chitin-derived oligosaccharides toxic to pathogenic bacteria using chia74, an endochitinase native to bacillus thuringiensis.to demonstrate that an endochitinase (chia74) native to bacillus thuringiensis can be used to generate chitin-derived oligosaccharides (ogs) with antibacterial activity against a number of aetiological agents of disease, including bacteria that cause diarrhoeal and emetic syndromes in humans.201020557451
high prevalence and molecular characterization of extended-spectrum β-lactamase-producing proteus mirabilis strains in southern croatia.the aim of this study was to determine the prevalence and antibiotic resistance rates of extended-spectrum β-lactamase (esbl)-producing proteus mirabilis strains isolated from inpatients at the split university hospital (southern croatia) during a survey performed between 2005 and 2008. a total of 2152 consecutive isolates of p. mirabilis were isolated. the prevalence was 0.5 % in 2005 and increased significantly to 20.9 % by 2008. strains were most frequently isolated from urine (36.5 %) and br ...201020558587
performance of the oxoid m.i.c.evaluator strips compared with the etest assay and bsac agar dilution.the oxoid m.i.c.evaluator (m.i.c.e; thermo fisher scientific) comprises an antibiotic gradient on a plastic support. we compared its performance with etest--a similar product--using bsac agar dilution as a reference.201020562429
prevalence of asymptomatic bacteriuria in type 2 diabetic subjects with and without microalbuminuria.abstract:201020565718
antimicrobial susceptibility and beta-lactamase production of selected gram-negative bacilli from two croatian hospitals: mystic study results.the meropenem yearly susceptibility test information collection (mystic) programme is a global, longitudinal resistance surveillance network that monitors the activity of meropenem and compares its activity with other broadspectrum antimicrobial agents. we now report the antimicrobial efficacy of meropenem compared to other broad-spectrum agents within the selective gram-negative pathogen groups from two croatian hospitals investigated between 2002-2007. a total of 1510 gram-negative pathogens w ...201020566417
chromosomally encoded blacmy-2 located on a novel sxt/r391-related integrating conjugative element in a proteus mirabilis clinical isolate.integrating conjugative elements (ices) are mobile genetic elements that can transfer from the chromosome of a host to the chromosome of a new host through the process of excision, conjugation, and integration. although sxt/r391-related ices, originally demonstrated in vibrio cholerae o139 isolates, have become prevalent among v. cholerae isolates in asia, the prevalence of the ices among gram-negative bacteria other than vibrio spp. remains unknown. in addition, sxt/r391-related ices carrying g ...201020566768
synthesis and antimicrobial activities of some quaternary morpholinium chlorides.the synthesis and antimicrobial activity of 31 morpholinium chlorides, divided into five series depending on the substituents attached to the nitrogen atom, n-carboxyalkyl-morpholinium chlorides (1a-e), n-carbalkoxymethyl-n-methyl-morpholinium chlorides (2a-f), n-carbethoxymethyl-n-alkyl-morpholinium chlorides (3a-g), n-carbalkoxymethyl-n-dodecyl-morpholinium chlorides (4a-f) and n-carboxymethyl-n-alkyl-morpholinium chlorides (5a-g) is reported. the compounds investigated were tested for antimic ...201020568530
chemical composition of the sfe-co extracts from cajanus cajan (l.) huth and their antimicrobial activity in vitro and in vivo.the in vitro and in vivo antimicrobial activities of sfe-co₂(supercritical fluid extraction) extracts and ethanol extracts from cajanus cajan (l.) huth were investigated. the flavonoid compounds orientin, vitexin, isovitexin, pinostrobin and the stilbene cajaninstilbene acid were detected in sfe-co₂ extracts by hplc-dad. in vitro antimicrobial activities of the extracts were evaluated against eight microbial strains (the bacteria staphylococcus epidermidis, staphylococcus aureus, bacillus subtil ...201020576412
synthesis, antibacterial activity evaluation and qsar studies of novel dispiropyrrolidines.a series of novel dispiropyrrolidines have been synthesized through 1,3-dipolar cycloaddition of an azomethine ylide generated from sarcosine and isatin with the dipolarophile 3-benzylidene-1-methyl-pyrrolidine-2,5-dione. their antibacterial activity was evaluated against bacillus subtilis ncim 2718, staphylococcus aureus ncim5021, salmonella typhi ncim2501, pseudomonas aeruginosa ncim 5029 and proteus vulgaris ncim2813 by two fold dilution method. compound 6e exhibits reasonably good activity a ...201020580853
molecular and genetic analyses of the putative proteus o antigen gene locus.proteus species are well-characterized opportunistic pathogens primarily associated with urinary tract infections (uti) of humans. the proteus o antigen is one of the most variable constituents of the cell surface, and o antigen heterogeneity is used for serological classification of proteus isolates. even though most proteus o antigen structures have been identified, the o antigen locus has not been well characterized. in this study, we identified the putative proteus o antigen locus and demons ...201020581173
overexpression and characterization in bacillus subtilis of a positionally nonspecific lipase from proteus vulgaris.a proteus vulgaris strain named t6 which produced lipase (pvl) with nonpositional specificity had been isolated in our laboratory. to produce the lipase in large quantities, we cloned its gene, which had an opening reading frame of 864 base pairs and encoded a deduced 287-amino-acid protein. the pvl gene was inserted into the escherichia coli expression vector pet-dsba, and active lipase was expressed in e. coli bl21 cells. the secretive expression of pvl gene in bacillus subtilis was examined. ...201020490605
volatiles emitted from eight wound-isolated bacteria differentially attract gravid screwworms (diptera: calliphoridae) to oviposit.bovine blood inoculated with bacteria isolated from screwworm [cochliomyia hominivorax (coquerel) (diptera: calliphoridae)]-infested animal wounds was tested as an attractant for oviposition for gravid screwworms. eight species of gram-negative coliform (enterobacteriaceae) bacteria mixed with bovine blood singly or all species combined and incubated for various times produced volatiles that attracted gravid flies in a cage bioassay in varying numbers. in 15-min duration tests, volatiles from fi ...201020496582
regulation of gene expression during swarmer cell differentiation in proteus mirabilis.the gram-negative bacterium proteus mirabilis can exist in either of two cell types, a vegetative cell characterized as a short rod and a highly elongated and hyperflagellated swarmer cell. this differentiation is triggered by growth on solid surfaces and multiple inputs are sensed by the cell to initiate the differentiation process. these include the inhibition of flagellar rotation, the accumulation of extracellular putrescine and o-antigen interactions with a surface. a key event in the diffe ...201020497230
pulmonary manifestations and management of proteus syndrome.proteus syndrome is a very rare, sporadic and congenital condition that is characterized by postnatal mosaic overgrowth. this disorder is thought to be caused by a somatic gene mutation, but the exact etiology is unknown. commonly involved tissues include connective tissue, bone, skin and the central nervous system. another less common symptom involves pulmonary emphysematous changes. this report documents a 25-year-old man with proteus syndrome who presented with progressive exertional dyspnea ...201020497874
may chlamydia trachomatis be an aetiological agent of chronic prostatic infection?chlamydia trachomatis infection is the most common sexually transmitted bacterial disease. the objective of this study was to establish the presence/absence of c. trachomatis in 98 patients with chronic complaints about the prostate and to evaluate the role of this bacterium in the inflammation of the gland. we performed culture and microscopical examination of pre-massage/post-massage urine and expressed prostatic secretions (eps). in all cases, culture on mccoy cells and polymerase chain react ...201020500746
microbial colonization of tracheoesophageal voice prostheses (provox2) following total laryngectomy.the purpose of this study was to determine the presence of individual microorganisms and the most frequent microbial combinations in the biofilm of the indwelling provox2 voice prosthesis in situ. furthermore, we wanted to evaluate the possible influence of biofilm composition on the mean and median lifetime of these voice prostheses. over a 5-year period, implantation of a provox2 voice prosthesis was performed in 85 patients, or 90% of the overall number of patients who underwent total larynge ...201020432044
antimicrobial susceptibility patterns of community-acquired uropathogens in tehran, iran.antibiotic resistance of urinary tract pathogens has increased worldwide. knowledge of the antibiotic resistance patterns of uropathogens in specific geographical locations is an important factor for choosing an appropriate empirical antimicrobial treatment. the aim of this study was to provide information regarding local resistance patterns of urinary pathogens to the commonly used antibiotics in tehran, iran.201020440056
neurognostics question. metamorphosis of a man: diagnosing joseph merrick. proteus syndrome. 201020446160
antimicrobial activity and a sar study of some novel benzimidazole derivatives bearing hydrazone moiety.in this study 12 novel benzimidazole compounds bearing hydrazone moiety were synthesized in order to investigate their possible antibacterial and antifungal activity. structures of the synthesized compounds were elucidated by spectral data. six different gram-negative and four different gram-positive bacterial strains were used in antibacterial activity tests. antifungal activity tests were also performed against three different fungal strains. most of the test compounds found to be significantl ...201020451306
osteomyelitis and osteonecrosis after intraosseous perfusion with gentamicin.to describe and discuss previously unreported complications associated with intraosseous perfusion with gentamicin in horses. study201020459490
introducing the scanning air puff tonometer for biological studies.it is getting increasingly evident that physical properties such as elastoviscoplastic properties of living materials are quite important for the process of tissue development, including regulation of genetic pathways. measuring such properties in vivo is a complicated and challenging task. in this paper, we present an instrument, a scanning air puff tonometer, which is able to map point by point the viscoelastic properties of flat or gently curved soft materials. this instrument is an improved ...201020365608
transcriptome of swarming proteus mirabilis.swarming motility by the urinary tract pathogen proteus mirabilis has been a long-studied but little understood phenomenon. on agar, a p. mirabilis colony grows outward in a bull's-eye pattern formed by consecutive waves of rapid swarming followed by consolidation into shorter cells. to examine differential gene expression in these growth phases, a microarray was constructed based on the completed genome sequence and annotation. rna was extracted from broth-cultured, swarming, and consolidation- ...201020368347
the encrustation and blockage of long-term indwelling bladder catheters: a way forward in prevention and control.to review the literature showing that understanding how foley catheters become encrusted and blocked by crystalline bacterial biofilms has led to strategies for the control of this complication in the care of patients undergoing long-term indwelling bladder catheterization.201020368711
from proteus to prometheus: learning from fish to modulate regeneration. 201020378822
loss of the waal o-antigen ligase prevents surface activation of the flagellar gene cascade in proteus mirabilis.proteus mirabilis is a gram-negative bacterium that undergoes a physical and biochemical change from a vegetative swimmer cell (a typical gram-negative rod) to an elongated swarmer cell when grown on a solid surface. in this study, we report that a transposon insertion in the waal gene, encoding o-antigen ligase, blocked swarming motility on solid surfaces but had little effect on swimming motility in soft agar. the waal mutant was unable to differentiate into a swarmer cell. differentiation was ...201020382766
zinc uptake contributes to motility and provides a competitive advantage to proteus mirabilis during experimental urinary tract infection.proteus mirabilis, a gram-negative bacterium, represents a common cause of complicated urinary tract infections in catheterized patients or those with functional or anatomical abnormalities of the urinary tract. znub, the membrane component of the high-affinity zinc (zn(2+)) transport system znuacb, was previously shown to be recognized by sera from infected mice. since this system has been shown to contribute to virulence in other pathogens, its role in proteus mirabilis was investigated by con ...201020385754
[urinary tract infections caused by extended-spectrum beta-lactamase (esbl)--producing enterobacteria in outpatients from primorsko-goranska county, croatia].the aim of this study was to report the prevalence of uropathogens and the frequency of extended spectrum beta-lactamases (esbl) producing strains isolated from urine of outpatients in primorsko-goranska county in croatia. we have retrospectively analyzed the results of 44, 321 urine cultures from january 01, 2008 till june 30, 2009. the study showed that esbl production was confirmed in 189 (1.8%) of the total of 10,757 isolates. rates of esbl-producing isolates were 19%, 0,6%, and 5.2% for kle ...201020387729
synthesis and antibacterial activity of carvacryl ethers.structural modifications of phenolic monoterpenoid obtained by reacting carvacrol with various substituted alpha-chloro acetanilides, to improve biological activities which give the product with better yield and higher purity under mild reaction conditions with the help of microwave irradiation techniques.201020390755
antibacterial and antifungal activities of (beta)-carboline alkaloids of peganum harmala (l) seeds and their combination effects.the β-carboline alkaloids of peganum harmala l were extracted through a bioassay-guided fractionation and their antimicrobial activities were investigated. results revealed significant differences (p>0.05) between compounds depending on the microorganism tested and the application method. when examined individually, harmine was the most effective against proteus vulgaris, bacillus subtilis and candida albicans where inhibition zones ranged between 21.2 and 24.7 mm. potentiality of the alkaloids ...201020398742
differences in biofilm formation and aggregative adherence between beta-lactam susceptible and beta-lactamases producing p. mirabilis clinical isolates.biofilm formation of multidrug resistant (mdr) extended-spectrum beta-lactamase (esbetal) producing proteus mirabilis isolates from a long-term care and rehabilitation facility (ltcrf) in northern italy was evaluated. a total of 10 strains, 4/10 producing the acquired ampc beta-lactamase cmy-16, 3/10 producing the esbetal tem-92 and the remaining negative for the presence of beta-lactamase genes, were studied using standard adherence assays on titer plates. tests were performed in three differen ...201020402412
decolorization and biodegradation of reactive dyes and dye wastewater by a developed bacterial consortium.a bacterial consortium (consortium gr) consisting of proteus vulgaris ncim-2027 and micrococcus glutamicus ncim-2168 could rapidly decolorize and degrade commonly-used sulfonated reactive dye green he4bd and many other reactive dyes. consortium gr shows markedly higher decolorization activity than that of the individual strains. the preferable physicochemical parameters were identified to achieve higher dye degradation and decolorization efficiency. the supplementation of cheap co-substrates (e. ...201020407917
species differentiation of seafood spoilage and pathogenic gram-negative bacteria by maldi-tof mass fingerprinting.species differentiation is important for the early detection and identification of pathogenic and food-spoilage microorganisms that may be present in fish and seafood products. the main 26 species of seafood spoilage and pathogenic gram-negative bacteria, including aeromonas hydrophila, acinetobacter baumanii, pseudomonas spp., and enterobacter spp. among others, were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) of low molecular wei ...201020408567
pathogenicity of aeromonas hydrophila, klebsiella pneumoniae, and proteus mirabilis to brown tree frogs (litoria ewingii).bacterial dermatosepticemia, a systemic infectious bacterial disease of frogs, can be caused by several opportunistic gram-negative bacterial species including aeromonas hydrophila, chryseobacterium indologenes, chryseobacterium meningosepticum, citrobacter freundii, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, and serratia liquifaciens. here we determined the pathogenicity of 3 bacterial species (aeromonas hydrophila, klebsiella pneumoniae, and proteus mirabilis) associated ...201020412685
recovery and screening for antibiotic susceptibility of potential bacterial pathogens from the oral cavity of shark species involved in attacks on humans in recife, brazil.the number of incidents involving sharks and humans at beaches in recife, on the north-eastern brazilian coast, is among the highest worldwide. in addition, wound infections in survivors are common; but the nature and risk of the aetiological agents is unknown. in the present study, 81 potential bacterial pathogens were identified in the oral cavity of sharks involved in attacks in recife, and were subjected to antibiotic susceptibility tests using the standardized disc-diffusion method. the maj ...201020413619
the effect of ph and chemical preservatives on the growth of bacterial isolates from some nigerian packaged fruit juices.bacterial pathogens were isolated from some nigerian packaged fruit juices. the isolates were characterized and identified as bacillus licheniformis, aeromonas hydrophila, bacillus circulans, proteus morganii, pseudomonas cepacia, bacillus alvei and pseudomonas chlororaphis. the antibiotic susceptibility profile of the seven isolates was determined and it was discovered that 65% of the microorganisms isolated were resistance to the antibiotic used while 35% were sensitive. the effect of ph, benz ...201020415148
[characteristics of urinary tract infections in different patient subpopulations and depending on the bladder emptying system].to analyze the prevalence and etiology of urinary tract infection in patients with neurogenic bladder depending on the bladder emptying system used as compared to a population with no neurological impairment. to assess perception of urinary tract infection by patients with neurogenic bladder.201020416242
ureases as a target for the treatment of gastric and urinary infections.urease is known to be a major contributor to pathologies induced by helicobacter pylori and proteus species. in h pylori, urease allows the bacteria to survive in an acidic gastric environment during colonisation, playing an important role in the pathogenesis of gastric and peptic ulcers. ureolytic activity also results in the production of ammonia in close proximity to the gastric epithelium, causing cell damage and inflammation. in the case of proteus species (notably proteus mirabilis) infect ...201020418234
bacteriological study of aerobic isolates from plantar ulcers of paucibacillary leprosy patients.plantar ulcers commonly occur in leprosy patients, which usually recur and cause morbidity in such cases.201020418976
assessing the emergence of resistance: the absence of biological cost in vivo may compromise fosfomycin treatments for p. aeruginosa infections.fosfomycin is a cell wall inhibitor used efficiently to treat uncomplicated urinary tract and gastrointestinal infections. a very convenient feature of fosfomycin, among others, is that although the expected frequency of resistant mutants is high, the biological cost associated with mutation impedes an effective growth rate, and bacteria cannot offset the obstacles posed by host defenses or compete with sensitive bacteria. due to the current scarcity of new antibiotics, fosfomycin has been propo ...201020419114
antibiotic resistance in community-acquired urinary tract infections: prevalence and risk factors.this study aimed to identify the agents causing community-acquired urinary tract infections (cautis) and their resistance patterns and to investigate risk factors for esbl production.201020424552
bacterial contamination of boar semen affects the litter size.one hundred and fifteen semen samples were collected from 115 different boars from two farms in cuba. the boars belonged to five different breeds. evaluation of the semen sample characteristics (volume, ph, colour, smell, motility of sperm cells) revealed that they meet international standards. the samples were also tested for the presence of agglutinated sperm cells and for bacterial contamination. seventy five percent of the ejaculates were contaminated with at least one type of bacteria and e ...201020427136
proteus takayasu's arteritis with unusual intracranial granulomatosis as initial manifestation.takayasu's arteritis (ta) is an inflammatory vasculitis of aorta and its branches, its low incidence limited our recognition to this entity. we sometimes can confuse this desease with polyarteritis nodosa and other vasculitis when no conventional "big artery" involved in ta cases. here we report a 26-year-old man with takayasu's arteritis who presented with a provisional intracranial granulomatosis first and then saccular aneurysms between celiac trunk and arteria hepatica communis and many othe ...201020354858
distinct bacterial colonization patterns of escherichia coli subtypes associate with rheumatoid factor status in early inflammatory arthritis.the aetiology of ra is unknown; however, bacterial exposure, particularly to escherichia coli, proteus mirabilis and klebsiella pneumoniae, has been linked to disease pathogenesis. the strongest association was observed for rf(+) ra. we compare colonization patterns of these bacteria, and the anti-bacterial antibody levels in early onset rf(+) and rf(-) inflammatory arthritis.201020360042
from congenital dermal sinus tract to extensive epidural abscess of the spine. 201020301052
prevalence of proteus mirabilis in skin abscesses of the axilla.gram-positive organisms are the most common infectious etiologic agents in skin abscesses. empiric antimicrobial treatment choices are influenced by this knowledge. in this report, we describe a high prevalence of proteus mirabilis, a gram-negative bacillus, in skin abscesses of the axilla, which has potential implications for selection of antimicrobial therapy.201020303504
proteus in the world of proteins: conformational changes in protein kinases.the 512 protein kinases encoded by the human genome are a prime example of nature's ability to create diversity by introducing variations to a highly conserved theme. the activity of each kinase domain is controlled by layers of regulatory mechanisms involving different combinations of post-translational modifications, intramolecular contacts, and intermolecular interactions. ultimately, they all achieve their effect by favoring particular conformations that promote or prevent the kinase domain ...201020336692
an assessment of the antibacterial activity in larval excretion/secretion of four species of insects recorded in association with corpses, using lucilia sericata meigen as the marker species.the relative antibacterial activities of excretion/secretion (es) from two carrion-feeding insects, calliphora vicina robineau-desvoidy and dermestes maculatus degeer, and a detritivore, tenebrio molitor linnaeus, were compared to that of lucilia sericata meigen, a species with es of known antibacterial capacity, in order to explore the antimicrobial potential of other carrion and detritivore species. viable counts were used to assess time-kill of es against five bacterial species, staphylococcu ...201020307342
triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection.rapid and simple analysis for the multiple target pathogens is critical for patient management. ce-sscp analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields. the capability of separating ssdna molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram. in this study, we explored the potential of poly(ethyleneoxide)-pol ...201020309929
effectiveness and safety of colistin: prospective comparative cohort study.colistin has re-entered clinical use by necessity. we aimed to assess its effectiveness and safety compared with newer antibiotics.201020299494
worldwide experience with the use of doripenem against extended-spectrum-beta-lactamase-producing and ciprofloxacin-resistant enterobacteriaceae: analysis of six phase 3 clinical studies.the worldwide increase in fluoroquinolone-resistant and extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae pathogens has led to doripenem and other carbapenems assuming a greater role in the treatment of serious infections. we analyzed data from 6 phase 3 multinational doripenem clinical trials on ciprofloxacin-resistant enterobacteriaceae isolates consisting of all genera (cipre) and esbl-producing enterobacteriaceae isolates consisting of escherichia coli, klebsiella spp., an ...201020211892
management of chronic gangrenous mastitis in a 3-year-old cow using partial (quarter) mastectomy.bovine gangrenous mastitis is an acute or peracute condition involving one or more quarters of the cow's udder. it occurs infrequently, but when it occurs, mortality of the affected cows is high. a partial mastectomy of one quarter using a cranial epidural analgesia with 2% lignocaine is described to manage a gangrenous mastitis affecting only one quarter caused by proteus mirabilis (a gram-negative bacteria) which was not amenable to medical treatment. partial mastectomy can be a safe and effec ...201020213222
photoantimicrobials as a potential local approach to geriatric utis.to test the efficacy of acceptable photoantimicrobial agents against bacterial pathogens implicated in complicated urinary tract infection (uti) in comparison with conventionally employed antibacterials.201020214732
management of craniofacial hyperostosis in proteus syndrome.proteus syndrome is a rare congenital hamartomatous condition that is characterized by a wide range of malformations, occasionally involving the head and the face. skin and skeletal developmental malformations are common and may be manifested in significant physical anomalies. in this report, we describe the cases of 2 patients affected by this condition. for one patient, a recurrence occurred 4 years after the elimination of cranial hyperostosis. in the second patient, although we observed macr ...201020216456
bacterial colonization of chronic leg ulcers: current results compared with data 5 years ago in a specialized dermatology department.in nearly every chronic wound different bacteria species can be detected. nevertheless, the presence of such microorganisms is not necessarily obligatory associated with a delayed wound healing. but from this initially unproblematic colonization an infection up to a sepsis can arise in some patients. the aim of our clinical investigation was to analyse the spectrum of microbial colonization of patients with a chronic leg ulcer in our specialized dermatological outpatient wound clinic, and to com ...201020236200
repression of common bull sperm flora and in vitro impairment of sperm motility with pseudomonas aeruginosa introduced by contaminated lubricant.semen collected from clinically healthy bulls at an artificial insemination centre was examined for bacterial diversity. while bacteria that are normally present in the common flora of bovine semen were absent, such as mycoplasma sp., proteus sp. and corynebacterium sp., all semen samples contained an unusually high number of pseudomonas aeruginosa strains. analysis via pulsed field gel electrophoresis demonstrated that one particular p. aeruginosa strain, present in a sealed bottle of lubricant ...201019144031
antibacterial activities of crude extract of aloe barbadensis to clinically isolated bacterial pathogens.the antibacterial activity of aloe barbadensis was tested on clinically isolated bacterial pathogens i.e. enterococcus bovis, staphylococcus aureus, escherichia coli, proteus vulgaris, proteus mirabilis, pseudomonas aeruginosa, morganella morganii, and klebsiella pneumoniae causing infection in human being. ethanolic and aqueous extracts were used for the antibacterial effect, which was measured by the appearance of zone of inhibition. relatively higher mic concentrations were obtained for gram ...201019263248
prevalence of acquired ampc beta-lactamases in enterobacteriaceae lacking inducible chromosomal ampc genes at a spanish hospital from 1999 to 2007.in 2007, a significant increase in acquired ampc genes in enterobacteriaceae from 0.06% in 1999 to 1.3% was observed. proteus mirabilis showed the highest prevalence (0.95%) and cmy-2 was the most prevalent ampc enzyme (66.7%). other enzymes such as cmy-4, dha-1, acc-1, and three new enzymes called cmy-25, cmy-27 and cmy-40 were detected. seven out of the 117 isolates (6%) also produced an extended-spectrum beta-lactamase. as acquired ampc enzymes are likely to become a serious public health iss ...201019523051
detection of ampc beta-lactamases in escherichia coli, klebsiella spp., salmonella spp. and proteus mirabilis in a regional clinical microbiology laboratory.there are currently no standardized diagnostic tests available for the reliable detection of ampc beta-lactamases in klebsiella spp., escherichia coli, proteus mirabilis and salmonella spp. a study was designed to evaluate a confirmation disk test using cefotetan (ctt) and cefoxitin (fox) with phenylboronic acid (pba). it also investigated the most suitable screening concentrations of fox, ceftriaxone (cro) and ceftazidime (caz) for the detection of ampc beta-lactamases. a total of 126 control ( ...201019456838
prolonged bacteraemia caused by vim-1 metallo-beta-lactamase-producing proteus mirabilis: first report from italy.persistent bacteraemia arising from a case of post-operative mediastinitis as a result of a proteus mirabilis isolate, possessing two class 1 integrons carrying bla(vim-1) and aada2 gene cassettes located on chromosomal and plasmidic dna, respectively, is reported. despite the in vitro susceptibility to carbapenems, meropenem therapy failed, whereas the patient responded to treatment with cefepime plus amikacin. to our knowledge, this is the first report of metallo-beta-lactamase production in a ...201019624511
980-nm laser diode and treatment of subcutaneous mass in proteus-like syndrome. 201019627404
syndromes with focal overgrowth in infancy: diagnostic approach and surgical treatment.abstract syndromes with focal overgrowth are sporadic diseases and comprise proteus syndrome and congenital lipomatous overgrowth, vascular malformations, and epidermal naevi (clove) syndrome, and isolated hemihyperplasia. we describe 3 children classified according to standard criteria with dysregulated growth of various tissues that was excised, together with excess toes, and tumours. correct classification facilitates diagnosis and operations. interdisciplinary treatment and follow-up are rec ...201019634071
gram-negative bacteria associated with brewery yeasts: reclassification of obesumbacterium proteus biogroup 2 as shimwellia pseudoproteus gen. nov., sp. nov., and transfer of escherichia blattae to shimwellia blattae comb. nov.phylogenetic analyses of type and reference strains of obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16s rrna gene sequences and partial sequences of four protein-coding genes (fusa, leus, pyrg and rpob). both approaches revealed that o. proteus biogroup 1 strains were closely related to hafnia alvei. biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included escherichia blattae. phenotypic tests were ...201019661513
monozygotic twins discordant for vascular malformations and dysregulated growth.there is a large group of disorders characterized by non-cranial vascular malformations and a dysregulated growth, of which the prototype may be klippel-trenaunay syndrome (kts). the aetiology of kts and vascular malformations-dysregulated growth (vm-dg) syndromes resembling kts is obscure, but polygenic paradominant inheritance involving mutations or polymorphisms in two or more genes simultaneously, of which one (or more) are involved in (lymph)angiogenesis and the other(s) in growth regulatio ...201019716450
characterization and flocculation mechanism of high efficiency microbial flocculant tj-f1 from proteus mirabilis.the characterization and the flocculation mechanism of microbial flocculant tj-f1 with high flocculating activity, produced by proteus mirabilis from a mixed activated sludge, was investigated. mainly consisting of protein and acid polysaccharide, tj-f1 owns a molecular weight of 1.2 x 10(5)da, which brings strong van der waals forces and ample binding-sites. it contains carboxyl, hydroxyl, amino groups and hydrogen bonds preferred for the flocculation process. the key parameters influencing the ...201019773148
molecular analysis of intestinal microbiota of rainbow trout (oncorhynchus mykiss).the aim of this study was to evaluate different molecular tools based on the 16s rrna gene, internal transcribed spacer, and the rpob gene to examine the bacterial populations present in juvenile rainbow trout intestines. dna was extracted from both pooled intestinal samples and bacterial strains. genes were pcr-amplified and analysed using both temporal temperature gradient gel electrophoresis (ttge) and restriction fragment length polymorphism methods. because of the high cultivability of the ...201019780831
mastoid subperiosteal abscess as a first sign of unnoticed cholesteatoma in children.to present children who underwent mastoidectomy for congenital cholesteatoma presented as a subperiosteal abscess.201019814752
antibacterial, antifungal, and antiviral activities of some flavonoids.antibacterial and antifungal activities of six plant-derived flavonoids representing two different structural groups were evaluated against standard strains of escherichia coli, pseudomonas aeruginosa, proteus mirabilis, klebsiella pneumoniae, acinetobacter baumannii, staphylococcus aureus, enterococcus faecalis, bacillus subtilis and their drug-resistant isolates, as well as fungi (candida albicans, c. krusei) using the microdilution broth method. herpes simplex virus type-1 and parainfluenza-3 ...201019840899
unilateral ovarian dermoid cyst accompanied by an ipsilateral paratubal cyst in a girl with proteus syndrome discovered by laparoscopic surgery.proteus syndrome is a rare hamartomatous disorder characterized by the overgrowth of multiple tissues in a mosaic pattern. tumors of genitourinary tract in proteus syndrome are uncommon.201019833537
evaluation of new chromogenic substrates for the detection of coliforms.to evaluate a new range of chromogenic substrates for the detection of beta-galactosidase activity in coliforms and to compare their performance in agar media and broths.201019878523
ciprofloxacin-induced hemorrhagic vasculitis.ciprofloxacin-induced hemorrhagic vasculitis is a rare side effect. it has only been described in 10 case reports since 1989. however, recently we were confronted with two cases within 1 month. in one patient the vasculitis resolved after termination of the ciprofloxacin therapy; in the other patient the ciprofloxacin-induced hemorrhagic vasculitis was superimposed on a severe forefoot infection, leading to progressive gangrene and a below-knee amputation. ciprofloxacin is among the standard tre ...201019892516
rheumatoid arthritis, proteus, anti-ccp antibodies and karl popper.rheumatoid arthritis (ra) is a crippling joint disease affecting over 20 million people worldwide. the cause of ra is most probably linked to the triad of microbial trigger, genetic association and autoimmunity and can be explained using the philosophical method of karl popper or popperian sequences. ten "popper sequences" have been identified which point to the urinary microbe proteus mirabilis as the cause of ra: popper sequence 1 establishes that hla-dr4 lymphocytes injected into a rabbit evo ...201019895906
modification of n-glycosylation sites allows secretion of bacterial chondroitinase abc from mammalian cells.although many eukaryotic proteins have been secreted by transfected bacterial cells, little is known about how a bacterial protein is treated as it passes through the secretory pathway when expressed in a eukaryotic cell. the eukaryotic n-glycosylation system could interfere with folding and secretion of prokaryotic proteins whose sequence has not been adapted for glycosylation in structurally appropriate locations. here we show that such interference does indeed occur for chondroitinase abc fro ...201019900493
[clinical aspects and epidemiology of uncomplicated cystitis in women. german results of the aresc study].uncomplicated cystitis in women is among the most frequent infections in the community setting. the german results of the international aresc study are reported concerning clinical aspects, epidemiology, and antimicrobial susceptibility of uropathogens.201019943031
comparative study on characteristics of azo dye decolorization by indigenous decolorizers.this study provides a first attempt to explore indigenous strains with excellent decolorization capability from the most biodiverse region in taiwan for dye-bearing wastewater treatment. bacterial isolates were obtained via serial selections under selection pressure of the fungicide nystatin and model textile dye(s). according to profiles of protein expression and pcr-augmented 16s rrna gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to ty ...201019944603
[intracranial suppurations of otorhinolaryngological origin in children in senegal].intracranial suppurations (icss) are poorly assessed in sub-saharan africa, especially in children.201019951836
structural and serological studies of the o-polysaccharide of strains from a newly created proteus o78 serogroup prevalent in polish patients.seven proteus mirabilis strains from five polish patients (five isolates from urea and two from feces) appeared to be a bacterial clone widespread in hospitals, most probably due to nosocomial infection and autoinfection. enzyme-linked immunosorbent assay and western blot showed that lipopolysaccharides from all strains studied are serologically identical to each other but distinct from proteus lipopolysaccharides studied earlier and, hence, these strains could not be classified in any of the cu ...201020002745
detecting vim-1 production in proteus mirabilis by an imipenem-dipicolinic acid double disk synergy test. 201020007383
orbus hercynius gen. nov., sp. nov., isolated from faeces of wild boar, is most closely related to members of the orders 'enterobacteriales' and pasteurellales.a novel gammaproteobacterium, strain cn3(t), was isolated from the faeces of wild boar. strain cn3(t) was facultatively anaerobic and appeared coccoid or rod-shaped. the partial 16s rrna gene sequence determined for strain cn3(t) suggested a distant relationship with members of the orders 'enterobacteriales' and pasteurellales. the gene sequence showed highest similarity (90.3 %) with obesumbacterium proteus dsm 2777(t), a member of the family enterobacteriaceae. the closest relatives outside th ...201020023064
transfer of a phage t4 gene into enterobacteriaceae, determined at the single-cell level.the transfer range of phage genes was investigated at the single-cell level by using an in situ dna amplification technique. after absorption of phages, a phage t4 gene was maintained in the genomes of non-plaque-forming bacteria at frequencies of 10(-2) gene copies per cell. the gene transfer decreased the mutation frequencies in nonhost recipients.201020023087
in vitro antimicrobial activity of a commercial ear antiseptic containing chlorhexidine and tris-edta.minimum bactericidal concentrations (mbcs) of a commercial ear antiseptic containing chlorhexidine 0.15% and tris-edta (otodine) were determined by broth microdilution for 150 isolates representing the most common pathogens associated with canine otitis. the microorganisms were classified into three groups according to their levels of susceptibility. the most susceptible group included staphylococcus pseudintermedius, malassezia pachydermatis, streptococcus canis and corynebacterium auriscanis, ...201020030799
antimicrobial susceptibility of multidrug-resistant (mdr) and extensively drug-resistant (xdr) enterobacteriaceae isolates to fosfomycin.the advancing antimicrobial drug resistance among enterobacteriaceae renders the evaluation of potential novel therapeutic options necessary. we sought to evaluate the in vitro antimicrobial activity of fosfomycin against multidrug-resistant (mdr) enterobacteriaceae isolates. antimicrobial susceptibility to fosfomycin and 12 additional antibiotics of mdr enterobacteriaceae isolates collected between november 2007 and april 2009 at the university hospital of heraklion, crete, greece, was examined ...201020034765
proteus mirabilis sp. intestinal microflora grow in a dextran sulfate sodium-rich environment.the pathogenic mechanisms responsible for inflammatory bowel disease, especially ulcerative colitis (uc), are poorly understood. as an animal model, the oral administration of dextran sulfate sodium (dss) induces colitis, which exhibits several clinical and histological features similar to uc. however, the pathogenic factors responsible for dss-induced colitis and above all, the intestinal microflora in this colitis remain unclear. therefore, we investigated the relationships between dss and the ...201020043128
bladder hemangiomas and proteus syndrome: a rare clinical association.a case of bladder hemangiomas in association with proteus syndrome (ps) is described. ps is a rare hamartomatous disorder and comprises an association of verrucous epidermal nevi, infantile hemangiomas, lipoma-like subcutaneous hamartomas and asymmetrical overgrowth of almost any part of the body. to our knowledge, this is the second case reported of hematuria associated with bladder hemangiomas in ps.201020044313
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