PMID(sorted descending)
ossicular discontinuity and exostoses in proteus syndrome: a case report.proteus syndrome (ps) is a rare hamartomatous disorder characterized by mosaic overgrowth of multiple tissues that manifests early in life and is progressive. the presence of unilateral external auditory canal exostoses in a patient who is not a swimmer or surfer is suggestive of ps. however, hearing loss is not a typical feature. here, we describe exostoses and ossicular discontinuity with conductive hearing loss in a patient with ps. the treatment consisted of canalplasty and ossicular chain r ...200515825577
technique for determining the bactericidal effect of drug combinations.two paper strips, each containing different antimicrobial agents, were placed on plates on mueller-hinton agar to permit antibiotic to enter the agar. a filter membrane was placed on this plate, and the microorganisms were planted on the membrane. after 6 h of incubation at 37 c, the membrane was transferred to antibiotic-free mueller-hinton agar containing triphenyltetrazolium hydrochloride and incubated for 18 h at 37 c. specific growth patterns were indicative of additive (indifferent), syner ...197415825416
potential unreliability of nitrofurantoin disks in susceptibility testing.the kirby-bauer disk diffusion antimicrobial susceptibility test was studied with three lots of commercial nitrofurantoin disks by using 50 strains of proteus mirabilis. one lot of disks consistently gave zone diameters 3 to 6 mm larger than the other two and resulted in a substantial number of proteus mirabilis strains being erroneously categorized as sensitive. antimicrobial content in disks of the three lots did not account for these differences. the ph of eluate from the unreliable disks was ...197415825413
synergy between cephalosporin and aminoglycoside antibiotics against providencia and proteus.clinical isolates of providencia and proteus with relative aminoglycoside resistance were tested for susceptibility to combinations of gentamicin or tobramycin with cephalothin or cefazolin. the minimal bactericidal concentration of aminoglycoside for one-third of the strains was reduced by fourfold or more in the presence of one-fourth of the minimal bactericidal concentration of either cephalosporin. this effect was achieved by clinically attainable concentrations of cephalothin or cefazolin.197415825407
interaction of clindamycin and gentamicin in vitro.the minimal inhibitory concentrations of clindamycin and gentamicin alone and in combinations were determined by a microdilution method for 163 aerobic, facultative, and anaerobic clinical isolates. all 77 strains of staphylococcus aureus, diplococcus pneumoniae, streptococcus pyogenes, and anaerobic bacteria (except for three strains of clostridium) were inhibited by 1.6 mug or less of clindamycin per ml. gentamicin did not interfere with the activity of clindamycin within the range of concentr ...197415825309
molecular diversity of proteus mirabilis isolates producing extended-spectrum beta-lactamases in a french university hospital.between february 1997 and december 2002, 3340 hospitalised patients yielded samples positive for proteus mirabilis, of whom 45 (1.3%) were colonised/infected by p. mirabilis producing extended-spectrum beta-lactamases (esbls). the gross incidence of patients colonised/infected by esbl-producing p. mirabilis was 1.61/10(5) days of hospitalisation, with 20% of isolates being collected from patients in urology wards, most frequently (53.3%) from urine samples. seventeen (37.7%) of the 43 isolates w ...200515819867
subset of individuals with autism spectrum disorders and extreme macrocephaly associated with germline pten tumour suppressor gene mutations.the genetic aetiology of autism remains elusive. occasionally, individuals with cowden syndrome (a cancer syndrome) and other related hamartoma disorders such as bannayan-riley-ruvalcaba syndrome, proteus syndrome, and proteus-like conditions, are characterised by germline pten mutations, and may have neurobehavioural features resembling autism as well as overgrowth and macrocephaly. therefore, we undertook pten gene mutation analysis in 18 subjects mainly prospectively ascertained with autism s ...200515805158
incidental detection of purulent fluid in kidney at percutaneous nephrolithotomy for branched renal calculi.some patients undergoing percutaneous nephrolithotomy (pcnl) have purulent fluid in the pelvicaliceal system at the time of puncture, although preoperative features are not suggestive of infection. we report the management and outcome of 19 such patients.200515798405
[resistance of catheter-associated urinary tract infections to antibacterials].catheter-associated urinary tract infections (cauti) are the most common nosocomial infections. the worldwide data show the increasing resistance to conventional antibiotics among urinary tract pathogens.200515790045
the antimicrobial activity of extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent.the antimicrobial activity and the mic values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. at least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus vulgaris, salmonella ...200515787241
evaluation of antimicrobial activity of beta-lactam antibiotics using etest against clinical isolates from 60 medical centres in antimicrobial resistance surveillance study was carried out in 60 medical centres across japan. resistance to piperacillin was 10.8% in clinical isolates of escherichia coli, while 1.3% or fewer isolates were resistant to other beta-lactams. klebsiella spp. were more susceptible to imipenem, cefepime and cefpirome. isolates of enterobacter spp., citrobacter spp., indole-positive proteus and serratia spp. were susceptible to imipenem, cefepime and cefpirome, while acinetobacter spp. were most ...200515784308
leucocyte migration and nitroblue tetrazolium assay in nigerian children with bacteremia and malaria parasitemia.the prevalence of malaria parasitemia, bacteremia, certain hematological parameters, leucocyte migration index and nitroblue tetrazolium dye reduction were determined in 147 nigerian children (4.24+/-2.88 years of age). sixty (40.8%), 28(19.1%) and 26(17.7%) had malaria parasitemia only, bacteremia only and both malaria parasitemia and bacteremia, respectively. four genera of bacteria, i.e e. coli, proteus, staphylococcus and salmonella, were detected in subjects with both malaria parasitemia an ...200415783134
malignant mimickers: chronic bacterial and fungal infections of the larynx.chronic infections of the larynx are notorious "copycats" of squamous cell carcinoma. patients typically present with a historical picture and symptoms identical to those seen in a neoplastic setting: dyspnea, hoarseness, odynophagia, weight loss, and a history of tobacco and alcohol abuse. historically, these patients were subject to an extensive resection for what was in reality a benign disease. a better understanding and awareness of these conditions has reinforced the need for a direct lary ...200515766861
[hydrolytic activity of marine bacteria associated with the mussel mytilus trossulus].the ability to produce hydrolytic enzymes--dnases, rnases, alkaline phosphatases, chitinases has been studied in 316 strains of bacteria isolated from mussel mytilus trossulus. strains of vibrio spp. produced all the spectrum of examined enzymes, bacteria of pseudoalteromonas genus produced alkaline phosphatase and dnase. chitin-degrading enzymes were found in vibrio and cytophaga--flavobacterium--bacteroides group. bacillus and vibrio spp. strains produced most actively rnases. the presence of ...200515765877
[urinary tract infections in children--etiologic agents and susceptibility to antibiotics].urinary tract infections (utis) are common clinical problem in pediatric hospitals. the aim of this study was to compare the incidence of utis during two different time periods (from february 1999 to march 2000, and from april 2000 to september 2001) in pediatric patients treated in ambulatory or hospitalized in new children hospital. the frequency of occurrence of esbl--producing gram-negative rods increased during second period study. the isolated esbl--producing strains were resistant also to ...200415765758
synthesis of some 1-[(n, n-disubstituted thiocar bamoylthio)acetyl]-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives and investigation of their antibacterial and antifungal activities.fourteen new 1-[(n, n-disubstituted thiocarbamoylthio)acetyl]-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives (7a-n) were synthesised by reacting 1-(chloroacetyl)-3-(2-thienyl)-5-aryl-2-pyrazolines (5a-g) and appropriate sodium salts of n, n-disubstituted dithiocarbamoic acids (6a, b). the structures of the synthesised compounds were confirmed by elemental analyses, uv, ir, (1)h-nmr and fab(+)-ms spectral data. their antibacterial activities against proteus vulgaris (nrrl b-123), escherichia coli ...200515765490
chlorine covers on living bacteria: the initial step in antimicrobial action of active chlorine compounds.although active chlorine compounds are well-known antimicrobial agents in human medicine, their initial steps of action have not been completely clarified. using n-chlorotaurine (nct), an endogenous mild representative, we observed persisting oxidation capacity affixed to bacteria. it was the aim of this study to investigate this 'chlorine cover'.200515761074
(1)h nmr spectroscopy in the diagnosis of pseudomonas aeruginosa-induced urinary tract infection.the utility of (1)h nmr spectroscopy is suggested and demonstrated for the diagnosis of pseudomonas aeruginosa in urinary tract infection (uti). the specific property of p. aeruginosa of metabolizing nicotinic acid to 6-hydroxynicotinic acid (6-ohna) is exploited. the quantity of 6-ohna produced correlates well with the viable bacterial count. other common bacteria causing uti such as escherichia coli, klebsiella pneumonia, enterobacter aerogenes, acinetobacter baumanii, proteus mirabilis, citro ...200515759292
interleukin-8 response in cells from the human urinary tract induced by lipopolysaccharides of proteus mirabilis o3 and o18.proteus mirabilis is a common pathogen associated mainly with complicated urinary tract infections and sometimes with septicemia. there is great serological diversity of the microorganism. while p. mirabilis o3 is commonly found in patients with infections, the serotype o18 rarely occurs. the o18 lipopolysaccharide contains a phosphocholine substitute, which makes it unique among proteus strains. to explain different clinical significance of the strains we evaluated the biological activity of th ...200515758809
[enzymatic resistance to beta lactam antibiotics within the genus proteus and evaluation of proteus mirabilis phenotypes and genotypes for resistance to third- and fourth-generation cephalosporins].the aim of this study was to evaluate betalactam resistance within the genus proteus and characterize the betalactamases responsible for this resistance.200515757582
[prevalence of proteus mirabilis strains in clinical specimens and evaluation of their resistance to selected antibiotics].proteus mirabilis are opportunistic microorganisms which cause serious hospital infections. the resistance of 1038 strains of p. mirabilis to selected antibiotics (lactams, aminoglycosides, fluoroquinolones, nitrofurantoin, cotrimoxazole, tetracycline) was tested by disk diffusion method. the least of p. mirabilis strains were resistant to imipenem and aztreonam. most of p. mirabilis strains were isolated from urine, wounds and ulcers from patients of departments of urology, dermatology, general ...200415754653
susceptibility testing of urinary isolates of escherichia coli to mecillinam using nccls methodology.criteria for susceptibility testing of mecillinam against 533 isolates of escherichia coli and a further 309 enterobacteriaceae, according to nccls methodology, were determined. correlation of mic to inhibition zones was good for all species. for urinary isolates of e. coli, the following agar dilution breakpoints and corresponding interpretive zone diameters seem appropriate: < or = 8 mg/l/> or = 15 mm for susceptible; 16 mg/l/12-14 mm for intermediate susceptible and > or = 32 mg/l/< or = 11 m ...200515737512
identification and functional characterisation of genes and corresponding enzymes involved in carnitine metabolism of proteus sp.enzymes involved in carnitine metabolism of proteus sp. are encoded by the cai genes organised as the caitabcdef operon. the complete operon could be sequenced from the genomic dna of proteus sp. amino acid sequence similarities and/or enzymatic analysis confirmed the function assigned to each protein involved in carnitine metabolism. cait was suggested to be an integral membrane protein responsible for the transport of betaines. the caia gene product was shown to be a crotonobetainyl-coa reduct ...200515731894
survey of the susceptibility of urinary isolates to antibacterial agents in 2003, 826 uropathogenic strains were obtained from the urine of our patients. we assessed their activities to antibacterial agents based on the minimal inhibitory concentration (mic) breakpoints recommended by the national committee for clinical standards (nccls). methicillin-resistant strains accounted for about 70% of staphylococcus aureus and staphylococcus epidermidis. the most common gram-positive species was enterococcus faecalis (15.6%). enterococcus faecium was the most resistant of t ...200515729488
rho/rho-dependent kinase affects locomotion and actin-myosin ii activity of amoeba proteus.the highly motile free-living unicellular organism amoeba proteus has been widely used as a model to study cell motility. however, the molecular mechanisms underlying its unique locomotion are still scarcely known. recently, we have shown that blocking the amoebae's endogenous rac- and rho-like proteins led to distinct and irreversible changes in the appearance of these large migrating cells as well as to a significant inhibition of their locomotion. in order to elucidate the mechanism of the rh ...200415726816
acute recurrent diverticulitis is prevented by oral administration of a polybacterial lysate suspension.the main cause of acute diverticulitis is the abnormal accumulation of fecal bacteria within the diverticular lumen, leading to a balancing between normal probiotic microflora and pathogenic species; gram negative entero-bacteriaceae, mainly escherichia coli and proteus spp, are the genders that usually cause the disease-related symptoms, due to their ability to adhere to intestinal mucosa. the intestine is well known as the largest human lymphoepithelial organ and daily produces more antibodies ...200415722985
pharmacodynamics of ibafloxacin in micro-organisms isolated from cats.the pharmacodynamic properties of ibafloxacin were investigated in micro-organisms isolated from cats. minimal inhibitory concentrations (mic) of ibafloxacin (racemate, r- and s-enantiomers) and its metabolites (7-hydroxy- and 8-hydroxy-ibafloxacin) and time-kill kinetics were determined against gram-negative and gram-positive bacteria isolated from dermal and respiratory and urinary tract infections in cats. racemic ibafloxacin has a broad spectrum of bactericidal activity against gram-negative ...200515720512
isolation of bacteria with antibiotic resistance from household cockroaches (periplaneta americana and blattella germanica).cockroaches may harbor and disseminate microorganisms to the environment. in this study, periplaneta americana and blattella germanica were collected from 40 households in kaohsiung city and kaohsiung county, taiwan. cockroach infestation was found in 50% of the studied households and 226 cockroaches (123 p. americana and 103 b. germanica) collected by trapping. p. americana was more often found in the kitchen (70.7%) whereas b. germanica in the storage room (51.5%) and kitchen (36.9%). there wa ...200515716054
structure of the o-polysaccharide and serological studies of the lipopolysaccharide of proteus penneri 60 classified into a new proteus serogroup alkali-treated lipopolysaccharide of proteus penneri strain 60 was studied by chemical analyses and 1h, 13c and 31p nmr spectroscopy, and the following structure of the linear pentasaccharide-phosphate repeating unit of the o-polysaccharide was established: 6)-alpha-d-galp-(1-->3)-alpha-l-fucpnac-(1-->3)-alpha-d-glcpnac-(1-->3)-beta-d-quip4nac-(1-->6)-alpha-d-glcp-1-p-(o--> rabbit polyclonal o-antiserum against p. penneri 60 reacted with both core and o-polysaccharide moieties of the homologo ...200515708308
otomycoses of candidal origin in eastern slovakia.mycological analysis of swabs and scraping samples from the external ear canals of 40 patients with clinically diagnosed otomycosis (10 neonates, 30 adults) revealed the presence of fungi as etiological agents. they were investigated microscopically using 20 % potassium hydroxide, and by cultivation on sabouraud's glucose agar. the candida species were identified using the germ-tube test, micromorphology observations of colonies on rice agar, and particularly by the commercial kit auxacolor. the ...200415702553
[etiology and antimicrobial susceptibility among uropathogens causing community-acquired lower urinary tract infections: a nationwide surveillance study].knowledge of antimicrobial susceptibility patterns is required to prescribe empirical therapy and formulate guidelines for the treatment of community-acquired urinary tract infections. this multicenter study assesses the etiology and antimicrobial susceptibility of the main community-acquired uropathogens in spain.200515701325
[distribution and drug-resistance of 3 500 gram-negative bacteria in guangzhou].to investigate the distribution and drug-resistance of the common gram-negative bacteria in guangzhou.200515698988
longitudinal trends in antimicrobial susceptibilities across long-term-care facilities: emergence of fluoroquinolone resistance.antibiotic resistance in the longterm-care facility (ltcf) setting is of increasing concern due to both the increased morbidity and mortality related to infections in this debilitated population and the potential for transfer of resistant organisms to other healthcare settings. longitudinal trends in antibiotic resistance in ltcfs have not been well described.200515693409
chondroitinase abc i from proteus vulgaris: cloning, recombinant expression and active site identification.galags (galactosaminoglycans) are one subset of the gag (glycosaminoglycan) family of chemically heterogeneous polysaccharides that are involved in a wide range of biological processes. these complex biomacromolecules are believed to be responsible for the inhibition of nerve regeneration following injury to the central nervous system. the enzymic degradation of gag chains in damaged nervous tissue by cabc i (chondroitinase abc i), a broad-specificity lyase that degrades galags, promotes neural ...200515691229
pollution status of swimming pools in south-south zone of south-eastern nigeria using microbiological and physicochemical indices.microbiological and physicochemical characteristics of swimming pools in south eastern states of nigeria (akwa ibom and cross river) were investigated. the bacterial isolates included enterococcus faecalis, clostridium perfringens, bacillus cereus and escherichia coli. others were pseudomonas aeruginosa, staphylococcus aureus, proteus vulgaris and staphylococcus epidermidis, while fungal isolates were penicillium sp, rhizopus sp, aspergillus versicolor fusarium sp, trichophyton mentagrophytes, m ...200415691161
intracranial complications following mastoidectomy.mastoidectomy is a common surgical procedure in otology. however, postoperative complications of various degrees of severity may occur. we present 4 children who underwent mastoidectomy for middle ear and mastoid disease and developed postoperative intracranial complications. one child was operated on for brain abscess 1 week after the initial mastoidectomy. another child appeared with seizures 5 days after the initial mastoidectomy and a subdural empyema was drained during revision surgery. lar ...200415689642
structure and serological studies of the o-polysaccharide of proteus penneri 75 epitopes and subgroups of proteus serogroup o73.the o-specific polysaccharide of the lipopolysaccharide of proteus penneri strain 75 consists of tetrasaccharide-ribitol phosphate repeating units and resembles ribitol teichoic acids of gram-positive bacteria. the following structure of the polysaccharide was elucidated by chemical methods and 1h and 13c nmr spectroscopy: [structure in text] where rib-ol is ribitol. serological studies with polyclonal antisera showed that the same structure of the o-polysaccharide occurred in two strains: p. pe ...200515681143
antimicrobial activity of sesquiterpenes from the essential oil of juniperus thurifera.chemical analysis of the essential oil of juniperus thurifera wood led to the identification of fifty-eight sesquiterpene compounds, of which twenty-eight have been isolated, two of them being new natural cedrane derivatives (1 and 2). their structures were established on the basis of 1d nmr and 2d nmr spectra. the antimicrobial activities of different pure compounds of this oil were tested against nine microorganisms (gram-positive and gram-negative bacteria, and yeasts). seven of the tested co ...200515678376
functional characterization of contractile vacuole isolated from amoeba proteus.contractile vacuoles (cvs) released from cells of amoeba proteus were used to analyze its function in vitro. when cv was transferred to a hypertonic medium, its volume decreased within 10 sec. when it was subsequently returned to its original medium, it quickly started swelling. however, it ruptured before recovering its initial volume. these results suggested that the cv membrane is semi-permeable and that the fluid is collected by the osmotic gradient in vivo. the water permeability of membran ...200415665503
influence of various criteria for elimination of duplicates when calculating the prevalence and antibiotic susceptibility of microorganisms associated with urinary infections.the effect of applying various criteria for elimination of repeated isolates in the same patient on the prevalence and antibiotic susceptibility of microorganisms frequently associated with urinary infections were analyzed. when time criteria were applied, there was a decrease in the number of isolates of 13.4-27.5%, whereas when variations in antibiotic susceptibility were considered, between 4.3 and 20.3% of the isolates were eliminated. the absolute number of isolates varied considerably depe ...200515664489
antiviral and antimicrobial activities of new nitrobutane is known that some addition products of beta-nitrostyrenes exhibit potent antimicrobial activity. in order to investigate the effects of structural modifications on the biological properties, some new michael type addition products of beta-ethyl-beta-nitrostyrenes were synthesized. in this study, eight new 1-[(2-aminophenyl)thio]-1-phenyl-2-nitrobutane (2) derivatives were synthesized by addition of 2-aminothiophenol to the double bond of beta-ethyl-beta-nitrostyrenes (1). the chemical struct ...200415646374
enhanced biofilm-production in pathogens isolated from patients with rare metabolic disorders.biofilm-producing bacteria were isolated from the urine of 19 patients with very rare metabolic disorders including: hyperlactacidaemia (8 cases), sugar intolerance and gammopathy (1 case), cystinuria (2 cases), parkinson's disease (1 case), lipidaemia (2 cases), hyperaminoaciduria (1 case) and others (4 cases). a total of 34 strains were collected, gram-negative and gram-positive microorganisms were equally distributed among the slime-producing bacteria, with a prevalence of staphylococcus epid ...200415646050
rapid, species-specific detection of uropathogen 16s rdna and rrna at ambient temperature by dot-blot hybridization and an electrochemical sensor array.development of rapid molecular approaches for pathogen detection is key to improving treatment of infectious diseases. for this study, the kinetics and temperature-dependence of dna probe hybridization to uropathogen species-specific sequences were examined. a set of oligonucleotide probes were designed based on variable regions of the 16s gene of the escherichia coli, proteus mirabilis, klebsiella oxytoca, and pseudomonas aeruginosa. a universal bacterial probe and probes-specific for gram-posi ...200415639199
[bacterial causes of meningitis in newborns].bacterial meningitis jeopardizes the life of affected newborns and in survived children often leaves permanent sequels. the sooner the diagnosis is established and the therapy is started, the more a prosperous outcome can be expected. when the indication is established, lumbar punction should be performed. on the basis of the results of cytological and biochemical analyses of the cerebrospinal fluid (csf) that point to bacterial meningitis, the initial therapy should begin even before the result ...200115637989
[naso-subcutaneous application of the polycomponent vaccine vp-4 for the treatment of patients with bronchial asthma and chronic obstructive bronchitis].bacterial polycomponent vaccine (vp-4) earlier developed at the mechnikov research institute for vaccines and sera on the basis of antigenic complexes of klebsiella pneumoniae, proteus vulgaris, escherichia coli, staphylococcus aureus was used within the program of clinical trials, for the treatment of 30 patients aged 16-59 years with the atopic and mixed form of bronchial asthma and chronic obstructive bronchitis by the combined naso-subcutaneous application. a good therapeutic effect produced ...200415636137
microbial resistance to antibiotics used to treat urinary tract infections in mexican children.urinary tract infections are one of the most common bacterial infections in female children. this infection has a prevalence of 4.1 to 7.1% during infancy. the bacterial agents causing the urinary tract infection are escherichia coil (80-90%), followed by other gram negative germs such as klebsiella pneumoniae and enterococcus species and gram positive such as staphylococcus species. typical treatment for this infection is ampicillin or the mixture of trimethoprim and sulfamethoxazole (tmt/smx) ...200415633630
intracranial complications of otitis media: 15 years of experience in 33 patients.complications in the central nervous system (cns) from acute otitis media (aom) and chronic otitis media (com) are becoming fewer, although they still represent a challenge for early recognition, adequate treatment, and satisfactory results. this retrospective study analyzed clinical data and therapeutic options in 33 patients with intracranial involvement resulting from otitis media. important clinical features of intracranial complications and the sequence of the most efficient therapeutic man ...200515632907
nature of the promoter activated by c.pvuii, an unusual regulatory protein conserved among restriction-modification systems.a widely distributed family of small regulators, called c proteins, controls a subset of restriction-modification systems. the c proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of the protective methyltransferase when the genes enter a new cell. c proteins bind to conserved sequences called c boxes. in the pvuii system, the c boxes have been reported to extend ...200515629920
evaluation of a new screen agar plate for detection and presumptive identification of enterobacteriaceae producing extended-spectrum beta-lactamases.a new agar screen plate for extended-spectrum beta-lactamase (esbl) detection was evaluated with 50 clinical isolates of esbl-producing enterobacteriaceae species: enterobacter cloacae (n = 10), escherichia coli (n = 10), klebsiella oxytoca (n = 3), klebsiella pneumoniae (n = 25), and proteus mirabilis (n = 2). fecal samples were artificially inoculated with 2 concentrations (25 and 250 colony forming units [cfu]/plate) of the test strains and then applied to the new agar screen plates. by this ...200515629229
[effect of delay of blood cultures on positive detection by automated blood culture system].the effect of entry delayed blood culture bottles until the start of incubation for mechanical detection of organism were compared using 2 major blood culture systems; bactec 9240 system and bact/alert 3d system. total of 13 bacterial strains; 5 gram-positive cocci, 7 gram-negative bacilli and candida parapsilosis which were isolated mainly from blood cultures were used as the test strains. bactec 92f, 93f and bact/alert fa, fn bottles were used as the blood culture bottles. all the bottles inoc ...200415628528
[relationship between antibacterial activity of aloe and its anthaquinone compounds].to investigate the relationship between the antibacterial activity of aloe and its contents of anthaquinone compounds, measure and compale antibacterial activities of aloin and aloe-emodin, and analyse the effect of glycoside on the antibacterial activity of aloin.200315615409
vascular anomalies and the growth of limbs: a review.growth of the limb in a child can be impaired, with the coexistence of a vascular malformation. in these vascular bone syndromes, altered growth is manifest as overgrowth or hypotrophy. the vascular malformation is usually complex and gets progressively worse with time. the two types of vascular anomalies in limbs, fast-flow and slow-flow, can be associated with limb length discrepancies. the fast-flow vascular malformations together with arteriovenous fistulae are part of parkes weber syndrome, ...200415599224
synthesis and study of antibacterial and antifungal activities of novel 2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl] acetylamino]thiazoles.several 2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl]acetylamino]thiazoles derivatives were synthesized by reacting 4-substituted-2-(chloroacetylamino)thiazoles with benzoxazole/benzimidazole-2-thioles in acetone and in the presence of k2co3. the chemical structures of the compounds were elucidated by ir, 1h-nmr, and fab(+)-ms spectral data. their antimicrobial activities against micrococcus luteus (nrll b-4375), bacillus cereus (nrrl b-3711), proteus vulgaris (nrrl b-123), salmonella typhimuriu ...200415595406
does the valve regulated release of urine from the bladder decrease encrustation and blockage of indwelling catheters by crystalline proteus mirabilis biofilms?we tested whether valve regulated, intermittent flow of urine from catheterized bladders decreases catheter encrustation.200515592093
clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle.bacteria contaminate the uterus of most dairy cattle after parturition and endometritis causes infertility. an endometritis score can be ascribed based on the vaginal mucus character and odour but it is not clear if the clinical score reflects the number of uterine bacteria or the inflammatory response. the present study tested the hypothesis that clinical evaluation of endometritis reflects the number of bacteria present in the uterus, and the acute phase protein response. swabs (n = 328) were ...200515589277
"craniofacial and dental manifestations of a case of proteus syndrome".proteus syndrome, which is a reference to the ancient greek god proteus, the polymorphous, was called to the attention of pediatricians. recognition of this syndrome has been difficult because of the variability of the syndrome's manifestations and because of its rarity. we describe a rare case of proteus syndrome and we discuss its differential diagnosis. our case presents with hemi facial hypertrophy, scrotal tongue, enamel hyperplasia as well as differential in the size of the dentition on bo ...200415573665
medical image. flank football. 200415570703
lanthanide spectroscopic studies of the dinuclear and mg(ii)-dependent pvuii restriction endonuclease.type ii restriction enzymes are homodimeric systems that bind four to eight base pair palindromic recognition sequences of dna and catalyze metal ion-dependent phosphodiester cleavage. while mg(ii) is required for cleavage in these enzymes, in some systems ca(ii) promotes avid substrate binding and sequence discrimination. these properties make them useful model systems for understanding the roles of alkaline earth metal ions in nucleic acid processing. we have previously shown that two ca(ii) i ...200415568821
cockroaches as potential vectors of nosocomial determine the possible role of cockroaches in the epidemiology of nosocomial infections.200415566034
phylogenetic characterization of legionella-like endosymbiotic x-bacteria in amoeba proteus: a proposal for 'candidatus legionella jeonii' sp. nov.the x-bacteria which initiated organismic association with the d strain of amoeba proteus in 1966 as parasites have changed to obligate endosymbionts on which the host depends for survival. owing to the difficulty in cultivating the bacteria in vitro, the identity of x-bacteria has not been determined. as the life cycle of x-bacteria is similar to that of legionella spp. in soil amoebae, we applied the polymerase chain reaction method with specific primers aimed at legionella spp. for the detect ...200415560823
[delayed brain abscess as a complication of a csf shunt].infection is a common complication of ventriculoperitoneal shunts, but the formation of a brain abscess related to shunt system is very rare. we present the case of a patient who developed a brain abscess around a ventricular catheter left in place after removing the valvular system.200415558205
use of translational fusion of the mrph fimbrial adhesin-binding domain with the cholera toxin a2 domain, coexpressed with the cholera toxin b subunit, as an intranasal vaccine to prevent experimental urinary tract infection by proteus mirabilis.this is a follow-up to our previous study using an intranasal vaccine composed of mrph, the tip adhesin of the mr/p fimbria, and cholera toxin to prevent urinary tract infection by proteus mirabilis (x. li, c. v. lockatell, d. e. johnson, m. c. lane, j. w. warren, and h. l. mobley, infect. immun. 72:66-75, 2004). here, we have expressed a cholera toxin-like chimera in which the mrph adhesin-binding domain (residues 23 to 157) replaces the cholera toxin a1 adp-ribosyltransferase domain. this chim ...200415557656
mannose-resistant proteus-like fimbriae are produced by most proteus mirabilis strains infecting the urinary tract, dictate the in vivo localization of bacteria, and contribute to biofilm formation.proteus mirabilis, an etiologic agent of complicated urinary tract infections, expresses mannose-resistant proteus-like (mr/p) fimbriae whose expression is phase variable. here we examine the role of these fimbriae in biofilm formation and colonization of the urinary tract. the majority of wild-type p. mirabilis cells in transurethrally infected mice produced mr/p fimbriae. mutants that were phase-locked for either constitutive expression (mr/p on) or the inability to express mr/p fimbriae (mr/p ...200415557655
modulation of swarming and virulence by fatty acids through the rsba protein in proteus mirabilis.after sensing external signals, proteus mirabilis undergoes a multicellular behavior called swarming which is coordinately regulated with the expression of virulence factors. here we report that exogenously added fatty acids could act as signals to regulate swarming in p. mirabilis. specifically, while oleic acid enhanced swarming, some saturated fatty acids, such as lauric acid, myristic acid, palmitic acid, and stearic acid, inhibited swarming. we also found that expression of hemolysin, which ...200415557604
beta-lactam susceptibilities and prevalence of esbl-producing isolates among more than 5000 european enterobacteriaceae vitro susceptibility to 15 beta-lactam antibiotics was evaluated using enterobacteriaceae isolated during the sentry antimicrobial surveillance program. piperacillin/tazobactam was the most active penicillin against escherichia coli, proteus mirabilis, klebsiella oxytoca and klebsiella pneumoniae (94.9%, 98.3%, 87.4% and 82.9% of isolates susceptible). of the cephalosporins, cefepime was most effective against escherichia coli, proteus mirabilis and enterobacter cloacae (99.2%, 96.3% and 95.2 ...200415555882
craniofacial hyperostoses in proteus syndrome -- a case report.description of a patient with a rare syndrome leading to the partial overgrowth of craniofacial bones.200415555524
nosocomial outbreak by proteus mirabilis producing extended-spectrum beta-lactamase veb-1 in a korean university examine the molecular mechanisms involved in the beta-lactam resistance of multidrug-resistant proteus mirabilis isolates that showed an unusual synergy between imipenem and ceftazidime in a korean hospital.200415546971
[infectious spondylodiscitis in a health area of galicia (spain) from 1983-2003].to study the clinical-epidemiological characteristics of infectious spondylodiscitis (is) in adults of our health area in the late 20 years.200415538902
bacterial isolates in the sick young infant in ile-ife, nigeria.this prospective study was carried out to determine the bacterial pathogens and their antibiotic sensitivity profile in the sick young infant. all consecutive young infants with features suggestive of infection seen over 5 months at a comprehensive health centre in ile-ife, were screened for septicaemia and local bacterial infections. of the 121 sick young infants screened for infection, 94 (77.7 per cent) had confirmed bacterial infection and 54 (57.4 per cent) of the 94 had confirmed septicaem ...200415537715
genetic and physiological interactions in the amoeba-bacteria symbiosis.amoebae of the xd strain of amoeba proteus that arose from the d strain by spontaneous infection of legionella-like x-bacteria are now dependent on their symbionts for survival. each xd amoeba contains about 42,000 symbionts within symbiosomes, and established xd amoebae die if their symbionts are removed. thus, harmful infective bacteria changed into necessary cell components. as a result of harboring x-bacteria. xd amoebae exhibit various physiological and genetic characteristics that are diff ...200415537083
endophthalmitis after pars plana vitrectomy: incidence, causative organisms, and visual acuity investigate the incidence, causative organisms, and visual acuity outcomes associated with endophthalmitis after pars plana vitrectomy.200415531315
effect of ceftazidime, amikacin and ciprofloxacin on biofilm formation by some enterobacterial clinical isolates.the effect of sub-minimal inhibitory concentrations of antimicrobial agents on the biofilm formationto polystyrene by escherichia coli, proteus vulgaris, providencia stuartii, and morganella morganii was investigated by examining eight clinical strains. all the isolates tested were efficient biofilm-forming strains in the microtiter plate assay, with crystal violet staining (od595 nm) ranging from 0.13 +/- 0.01 for p. stuartii er21870 to 1.23 +/- 0.02 for p. vulgaris er50120. the biofilm formati ...200415528892
intestinal microflora in 45 crows in ueno zoo and the in vitro susceptibilities of 29 escherichia coli isolates to 14 antimicrobial agents.microorganisms from 45 jungle crows (corvus macrorhynchos) captured from july to december 2002 at ueno zoo, tokyo were identified as escherichia coli, proteus mirabilis, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter cloacae, enterobacter agglomerans, pseudomonas maltophila, staphylococcus spp., micrococcus spp., and streptococcus spp. e. coli showed the highest rate of isolation (21.6%). in an in vitro susceptibility test for 29 isolates of e. coli to 14 antimic ...200415528866
antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens.the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ...200415527919
the bacteriology of open fractures in ile-ife, nigeria.the objective of this study is to determine the pattern of bacterial isolates and antibiotic sensitivity profile in open fractures. fifty-nine patients with open fractures of the lower limb long bones were studied. the majority of the cases were gustilo and anderson types ii 21(35.6%), and iiia 16(27.1%).200415523862
[chronic carriers of pathogen conjunctival bacteria. possible risks in cataract surgery].to ascertain the frequency and characteristics of chronic carriers of conjunctival pathogen bacteria among patients undergoing cataract surgery in our hospital, to allow the design of studies of their postsurgical endophthalmitis risk.200415523569
new structures of the o-specific polysaccharides of proteus. 4. polysaccharides containing unusual acidic n-acyl derivatives of 4-amino-4,6-dideoxy-d-glucose.the structures of the o-polysaccharides of the lipopolysaccharides of proteus mirabilis o7 and o49 were determined by chemical methods, mass spectrometry, including ms/ms, and nmr spectroscopy, including experiments run in an h2o/d2o mixture to reveal correlations for nh protons. the o-polysaccharides were found to contain n-carboxyacetyl (malonyl) and n-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, qui4n), respectively. the behavior of qui4 ...200415521818
rapidly rising prevalence of nosocomial multidrug-resistant, gram-negative bacilli: a 9-year surveillance examine and quantify the temporal trends of nosocomial multidrug-resistant, gram-negative bacilli.200415518026
detection of ticarcillin-clavulonic acid susceptibility with microdilution method in citrobacter, hafnia, proteus and some gram negative bacteria.the broth dilution method has been regarded as a good alternative test for detection of susceptibilities to antimicrobial agents. in this study, the antimicrobial activity of ticarcillin-clavulonic acid (tim) was investigated by the minimal inhibitory concentration (mic) method on strains of aeromonas, citrobacter, hafnia, morganella, proteus, pseudomonas and gram negative bacteria isolated from raw milk. the isolate collection included 91 gram negative strains. fifty-one (56.04%) isolates were ...200415508433
plasmid-mediated multiple antibiotic resistance in proteus mirabilis isolated from patients with urinary tract infection.worldwide surveillance of antimicrobial resistance among urinary tract pathogens is useful to determine important trends and geographical variation for common gram negative and positive species. we initiated this study to learn the drug resistance pattern and its location (genomic or plasmid-borne) among the strains of proteus mirabilis, one of the most common causative agents of urinary tract infection (uti).200415507850
traditional medicine in sakarya province (turkey) and antimicrobial activities of selected species.traditional medicine in north-west of turkey (sakarya province) were studied during a 2 months field study by interviewing local informants from several villages. plant species used to treat infections were tested for antimicrobial activity. information was collected for 46 plant species from 30 families and for 5 animal species. twenty four of the plant species were cultivated. most used families were asteraceae, cucurbitaceae, lamiaceae and rosaceae and the most used plants were artemisia absi ...200415507351
synthesis and antimicrobial activity of some netropsin analogues.nine novel lexitropsins were synthesized by linking two netropsin-like moieties through three different dicarboxylic acids; 9,10-dihydro-2,7-phenanthrenedicarboxylic acid; [(3-[[(carboxymethyl)amino]carbonyl]benzoyl)amino]acetic acid and indole-2,5-dicarboxylic acid. the netropsin residues were modified by the use of n-isopentylpyrrole, 5-methylthiophene or 5-isopropylthiazole heterocyclic building blocks in place of the usual n-methylpyrrole. the compounds were tested against five gram-positive ...200415505717
four variants of the citrobacter freundii ampc-type cephalosporinases, including novel enzymes cmy-14 and cmy-15, in a proteus mirabilis clone widespread in poland.twenty-nine proteus mirabilis isolates from 17 polish hospitals were analyzed. the isolates were resistant to a variety of antimicrobials, and their patterns of resistance to beta-lactams resembled those of the constitutive class c cephalosporinase (ampc) producers. indeed, beta-lactamases with a pi of approximately 9.0 were found in all of the isolates, and they were subsequently identified as four ampc-type cephalosporinases, cmy-4, -12, -14, and -15, of which the two last ones were novel enzy ...200415504832
microorganisms and calcium oxalate stone disease.microorganisms may have a role in the pathogenesis and prevention of kidney stones. the subjects of this review include nanobacteria, oxalobacter formigenes, and lactic acid bacteria. not reviewed here is the well-described role of infections of the urinary tract with proteus species and other urease-producing organisms associated with struvite stone formation. nanobacteria have been proposed to be very small (0.08-0.5 nm), ubiquitous organisms that could play a role in stone formation. the theo ...200415499215
micredox--development of a ferricyanide-mediated rapid biochemical oxygen demand method using an immobilised proteus vulgaris biocomponent.biochemical oxygen demand (bod) is an international regulatory environmental index for monitoring organic pollutants in wastewater and the current legislated standard test for bod monitoring requires 5 days to complete (bod5 test). we are developing a rapid microbial technique, micredox, for measuring bod by eliminating oxygen and, instead, quantifying an equivalent biochemical co-substrate demand, the co-substrate being a redox mediator. elevated concentrations of proteus vulgaris, either as fr ...200415494235
[microbiological quality of minimally processed vegetable salads].the microbiological condition of minimally processed vegetable salads (7-days durability) purchased in the retail network of szczecin has been assessed. the study included 14 kinds of salads manufactured by two producers. the total count of mesophilic aerobic bacteria and their spores, aid-forming bacteria (lactobacilli), titre of coliforms, occurrence of pathogenic bacteria and counts of moulds and yeast were determined. no pathogenic bacteria (salmonella, staphylococcus aureus, bacillus cereus ...200415493346
detection of staphylococcus aureus enterotoxin a and b genes with pcr-eia and a hand-held electrochemical sensor.two electrochemical assays for detecting staphylococcus aureus enterotoxin a and b genes were developed. the assays are based on pcr amplification with biotinylated primers, hybridization to a fluorescein-labeled probe, and detection with horseradish peroxidase-conjugated anti-fluorescein antibody using a hand-held electrochemical detector. the limit of detection (lod) for both assays was approximately 16 copies of the sea and seb genes. the assays were evaluated in blinded studies, each with 81 ...200415488376
[antibodies to yersinia enterocolitica and proteus mirabilis in blood sera of rheumatoid arthritis patients].data on specific antibodies to the bacterial antigens of yersinia enterocolitica (serovars 03, 09) and proteus mirabilis in rheumatoid arthritis (ra) patients are presented. according to the data of some researchers, these organisms play some role in the pathogenesis of ra. the highest level of igg antibodies to y. enterocolitica antigens of both serovars was noted. the activity of specific igg antibodies to serovar 03 was higher than that to serovar 09. in the sera of ra patients with p. mirabi ...200415481928
evaluation of the microscan esbl plus confirmation panel for detection of extended-spectrum beta-lactamases in clinical isolates of oxyimino-cephalosporin-resistant gram-negative bacteria.we aimed to assess the performance of the microscan esbl plus confirmation panel using a series of 87 oxyimino-cephalosporin-resistant gram-negative bacilli of various species.200415471997
digestion of cellulose and xylan by symbiotic bacteria in the intestine of the indian flying fox (pteropus giganteus).bats (order chiroptera) are a widely distributed group of mammals. pteropus giganteus belongs to the suborder megachiroptera. this bat consumes fruits and leaves as their major food. cellulose and xylan are the major composition of leaves. as they consume leaves in their diet, their digestive tract must contain cellulolytic and xylanolytic bacteria which help in the digestion of cellulose and xylan. the cellulolytic and xylanolytic bacteria were isolated and screened on berg's agar containing ce ...200415471682
correlation of extended spectrum beta lactamases production with cephalosporin resistance in gram negative bacilli.beta lactamase production is an important mechanism of developing resistance to beta lactam group of antibiotics. cephlosporins with extended spectrum of activity and stability were introduced to overcome this resistance, but soon production of extended spectrum beta lactamases (esbls), which are inducible in nature was reported. in this study klebsiella aerogenes--166, escherichia coli--120, citrobacter spps--116, pseudomonas spps--50, proteus spps--32 and s. typhi--16, strains were subjected t ...200415471144
[microbiocenosis of the bowels in patients with gastroduodenal ulcers].gastroduodenal ulcer is accompanied by the bowels dysbacteriosis irrespective of the helicobacter pylori infection. any variant of ulcer treatment aggravates the bowels dysbacteriosis intensity. eradication therapy destroys bifidobacteria mostly.200415462314
species-dependent premature degradation of absorbable suture materials caused by infection--impact on the choice of thread in vascular case of infection after vascular reconstruction, preference is often given to absorbable suture material with the aim of preventing persistence of infection. we have investigated the functional deficit of absorbable sutures on incubation with various different bacteria.200415461069
[antimicrobial activity of new original 2-aryliden-6-furfuryliden cyclohexanones and hexahydroindazoles on their basis].antimicrobial activity of 25 new original 2-aryliden-6-furfuryliden cyclohexanones and hexahydroindazoles based on them was studied. the majority of the compounds had low or moderate activity against the test cultures of staphylococcus spp., pseudomonas aeruginosa, proteus spp. and escherichia coil. compound xix (2,3-diphenyl-7-(5-nitrofurfuryliden)-3,3a,4,5,6,7-hexahydroindazole) with high antistaphylococcal activity was selected among several 2-aryliden-6-furfuryliden cyclohexanones and hexahy ...200415460019
pcr method based on the ogdh gene for the detection of salmonella spp. from chicken meat a previous paper, the ogdh gene that encodes 2-oxoglutarate dehydrogenase was isolated from salmonella typhimurium. the catalytic n-terminal region in the enzyme was found to be very specific for the salmonella species. therefore, the aim of the present study was to detect s. typhimurium in food sources using primers designed for ogdh-1 and ogdh-2 which were based on the salmonella-specific region of the ogdh gene. a simple polymerase chain reaction (pcr) detection method was developed to det ...200415459651
[bacterial colonization and home mechanical ventilation: prevalence and risk factors].to investigate the prevalence of bacterial contamination of ventilators and colonization of patients, the bacteria implicated, and predisposing factors in noninvasive home ventilation.200415458614
prulifloxacin.prulifloxacin, the prodrug of ulifloxacin, is a broad-spectrum oral fluoroquinolone antibacterial agent. after absorption, prulifloxacin is metabolised by esterases to ulifloxacin. the drug has a long elimination half-life, allowing once-daily administration. ulifloxacin is generally more active in vitro than other fluoroquinolones against a variety of clinical isolates of gram-negative bacteria, including community and nosocomial isolates of escherichia coli, klebsiella spp., proteus, providenc ...200415456336
otitis externa infection in jordan. clinical and microbiological features.aims of this study were to investigate the clinical findings and to identify causative microorganisms and their susceptibility to antibiotics in otitis externa infection.200415448765
thermorubin, a new antibiotic from a thermoactinomycete. 196415446134
incidence and susceptibility to antibiotics of bacteria causing common infections in a general hospital. 196315446089
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