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clinical features and long-term follow-up of quasi-moyamoya disease in children.background: inherited or acquired disorders and conditions may present in conjunction with moyamoya disease. this condition is known as quasi-moyamoya disease. methods: a retrospective review of 69 moyamoya disease patients treated for the past 20 years identified 7 patients with quasi-moyamoya disease and 5 of them were pediatric patients. results: the mean age at initial diagnosis was 6.4 years (range 5-9). associated disorders were: craniosynostosis, dwarfism with coarctation of aorta, proteu ...201121576916
[beta-lactamases of clinical isolates of enterobacteriaceae family].among 62 clinical strains of enterobacteria, isolated from intestinal tract, representatives of genus escherichia made--67.4%, klebsiella--4.84%, citrobacter--4.84%, enterobacter--8.06%, proteus--6.45%, morganella of 8.06%. preparations of fluorquinolone group--gatifloxacin and moxifloxacin, the number of steady strains being from 0.0% to 33.3%, were the most effective in respect of enterobacteria the investigated strains of enterobacteria showed considerable resistance to beta-lactam antibiotic ...201121598655
in vitro antimicrobial activity of ten medicinal plants against clinical isolates of oral cancer cases.abstract: background: suppression of immune system in treated cancer patients may lead to secondary infections that obviate the need of antibiotics. in the present study, an attempt was made to understand the occurrence of secondary infections in immuno-suppressed patients along with herbal control of these infections with the following objectives to: (a) isolate the microbial species from the treated oral cancer patients along with the estimation of absolute neutrophile counts of patients. (b) ...201121599889
screening of australian plants for antimicrobial activity against campylobacter jejuni.campylobacter jejuni is the most common cause of acute enteritis in humans, with symptoms such as diarrhoea, fever and abdominal cramps. in this study, 115 extracts from 109 australian plant species were investigated for their antimicrobial activities against two c. jejuni strains using an in vitro broth microdilution assay. among the plants tested, 107 (93%) extracts showed activity at a concentration between 32 and 1024 µg/ml against at least one c. jejuni strain. seventeen plant extracts were ...201121604309
surgical approach to extensive hidradenitis suppurativa.background hidradenitis suppurativa (hs) is a chronic suppurative disease of skin with high recurrence. objective to determine factors affecting complications and recurrence of hs in patients who underwent surgery. materials and methods we operated on 15 patients with hs at 36 sites between 1999 and 2009. the affected areas were classified as groin, axilla, buttocks, nuchae, perianal, and perineal. all patients were treated using wide surgical excision under general anesthesia. the methods of re ...201121605246
a novel compound from the marine bacterium bacillus pumilus s6-15 inhibits biofilm formation in gram-positive and gram-negative species.biofilm formation is a critical problem in nosocomial infections and in the aquaculture industries and biofilms show high resistance to antibiotics. the aim of the present study was to reveal a novel anti-biofilm compound from marine bacteria against antibiotic resistant gram-positive and gram-negative biofilms. the bacterial extract (50 μg ml(-1)) of s6-01 (bacillus indicus = mtcc 5559) showed 80-90% biofilm inhibition against escherichia coli, shigella flexneri, proteus mirabilis and s6-15 (ba ...201121614700
antimicrobial activity of protease inhibitor from leaves of coccinia grandis (l.) voigt.antimicrobial activity of protease inhibitor isolated from coccinia grandis (l.) voigt. has been reported. a 14.3 kda protease inhibitor (pi) was isolated and purified to homogeneity by ammonium sulfate precipitation (20-85% saturation), sephadex g-75, deae sepharose column and trypsin-sepharose affinity chromatography from the leaves of c. grandis. the purity was checked by reverse phase high performance liquid chromatography. pi exhibited marked growth inhibitory effects on colon cell lines in ...201121615062
embolic capture angioplasty of lower extremity lesion following distal embolization.peripheral embolization is a known complication of peripheral artery interventions. it can lead to occlusion of distal vessels leading to significant lower extremity ischemia and complications. peripheral artery interventions involving chronic total occlusions have been shown to have higher rates of complications including distal embolization. although distal embolic protection strategies are available, they are seldom utilized during lower extremity interventions, especially during treatment of ...201121616726
potentiation of antimicrobial activity of ciprofloxacin by pelargonium graveolens essential oil against selected uropathogens.the recent approach of using herbs and antibiotics in combination constitutes a strategy to overcome the problems of resistance and side effects associated with conventional antibiotics. in the present study, the antimicrobial effect of pelargonium graveolens l' hér essential oil in combination with ciprofloxacin was evaluated on uropathogens, namely, klebsiella pneumoniae kt2, proteus mirabilis prt3 and staphylococcus aureus st2. minimum inhibitory concentrations of p. graveolens essential oil ...201121618302
genereviewsthe pten hamartoma tumor syndrome (phts) includes cowden syndrome (cs), bannayan-riley-ruvalcaba syndrome (brrs), proteus syndrome (ps), and proteus-like syndrome. cs is a multiple hamartoma syndrome with a high risk for benign and malignant tumors of the thyroid, breast, and endometrium. affected individuals usually have macrocephaly, trichilemmomas, and papillomatous papules and present by the late 20s. the lifetime risk of developing breast cancer is 25%-50%, with an average age of diagnosis ...199320301661
ureaseurease is produced by numerous taxonomically diverse bacterial species, including normal flora and nonpathogens. also, urease has been demonstrated as a potent virulence factor for some species, including proteus mirabilis (51), staphylococcus saprophyticus (36), and helicobacter pylori (23). urease is central to h. pylori metabolism and virulence, is necessary for its colonization of the gastric mucosa, and is a potent immunogen that elicits a vigorous immune response. this enzyme is used for t ...200121290719
medical microbiologythe gram-negative bacilli of the genera escherichia, klebsiella, enterobacter, serratia, citrobacter,and proteus(table 26- 1) are members of the normal intestinal flora of humans and animals and may be isolated from a variety of environmental sources. with the exception of proteus, they are sometimes collectively referred to as the coliform bacilli because of shared properties, particularly the ability of most sp ...199621413290
intra-pericardial rupture of bacterial hepatic abscess: an unusual cause of 'cardiac' chest pain.we report a rare case of bacterial pericarditis secondary to rupture of liver abscess into the pericardium. the patient presented with cardiac-sounding chest pain and with normal ecg, cxr and troponin-i assay. the initial echocardiogram showed minimal pericardial fluid but, when he later developed overt clinical signs of cardiac tamponade, a ct scan of chest and abdomen revealed a pronounced pericardial effusion. it also revealed an abscess located in the left lobe of liver and this had apparent ...200621611623
new delhi metallo-ss-lactamase-1: local acquisition in ontario, canada, and challenges in detection.new delhi metallo-ß-lactamase-1 (ndm-1) is a recently identified metallo-ß-lactamase that confers resistance to carbapenems and all other ß-lactam antibiotics, with the exception of aztreonam. ndm-1 is also associated with resistance to many other classes of antibiotics. the enzyme was first identified in organisms isolated from a patient in sweden who had previously received medical treatment in india, but it is now recognized as endemic throughout india and pakistan and has spread worldwide. t ...201121624908
molecular analysis of ndm-1-producing enterobacterial isolates from geneva, switzerland.objectives to analyse the mechanisms responsible for decreased susceptibility or resistance to carbapenems in several enterobacterial isolates recovered in 2009-10 in geneva university hospitals, switzerland. methods pcr and sequencing were used to identify β-lactamases, 16s rna methylases and plasmid-mediated quinolone resistance genes. the transferable properties of the plasmids were analysed, as well as their plasmid type. the strains were typed by multilocus sequence typing. results three pa ...201121628303
superior in vitro activity of ertapenem and piperacillin/tazobactam against recent clinical isolates of proteus mirabilis from intra-abdominal infections (smart 2009-2010). 201121632217
setting interpretive breakpoints for antimicrobial susceptibility testing using disk diffusion.antimicrobial susceptibility testing plays a key role in clinical microbiology. the disk diffusion test dates back to the 1940s and became standardised from the 1950s, with the international collaborative study (ics) and national committee for clinical laboratory standards (nccls) as the two major standards. interlaboratory variation of disk test results was recognised early but has never been dealt with in a satisfactory manner. the error-rate bounded method was described in 1974 and its role i ...201121640560
recognition of dna by the helix-turn-helix global regulatory protein lrp is modulated by the amino terminus.the asnc/lrp family of regulatory proteins links bacterial and archaeal transcription patterns to metabolism. in escherichia coli lrp regulates approximately 400 genes, over 200 of them directly. in earlier studies, lrp genes from vibrio cholerae, proteus mirabilis, and e. coli were introduced into the same e. coli background, and yielded overlapping but significantly different regulons. these differences were seen despite amino acid sequence identities of 92% (vibrio) and 98% (proteus) to e. co ...201121642464
production of bioactive sheep β-defensin-1 in pichia pastoris.previous research has shown that sheep β-defensin-1 (sbd-1), a small cationic peptide with a broad range of antimicrobial activities, could inhibit the growth of both gram-positive and gram-negative bacteria as well as that of fungi. in order to increase the yield of current ovine defensin purification methods, mature sbd-1 (msbd-1) was added with a 6-his tag on the c-terminus (msbd-1-t) and expressed in pichia pastoris in the presented work. the msbd-1 and msbd-1-t were expressed in the pichia ...201121643707
isolation, selection and biodegradation profile of phenol degrading bacteria from oil contaminated soil.in the present study, an aerobic bacterial strains ocs-a and ocs- b were isolated from an oil contaminated soil. the strains were identified to be citrobacter freundi and proteus mirabilis according to morphological, physiological and biochemical characteristics. the strains were able to degrade about 90% of 100 mg/l phenol within 80 h as sole carbon and energy source. the lag phase increased with increase in phenol concentration. determination of metabolic intermediate 2-hms, was done which ind ...201121643832
synthesis of p-triazinylphosphonium salts--hybrid molecules with potential antimicrobial activity.the new hybrid drugs combining in a single molecule triazine ring attached to phosphonium salt were prepared and their bactericidal activity against gram-positive bacteria staphylococcus aureus atcc43300 (mrsa - methycyline resistant staphylococcus aureus strain) two gram-negative bacteria escherichia coli ccug31997 serotype o153 (epec - enteropathogenic escherichia coli strain), proteus mirabilis 1784 (mdr - multidrug resistant clinical strain) were determined using microdilution method.201121648193
pcb accumulation and tissue distribution in cave salamander (proteus anguinus anguinus, amphibia, urodela) in the polluted karstic hinterland of the krupa river, slovenia.for over two decades, a manufacturer of electrical capacitors disposed of its waste within the karstic hinterland of the krupa river (slovenia) resulting in the surroundings becomming heavily polluted with pcb. albeit the extent of the contamination has been known since 1983 and the krupa river has become one of the most pcb polluted river in europe, the effects on the cave fauna of the region remain unknown. the most famous cave dweller of the krupa hinterland is the endemic cave salamander pro ...201121658741
jacaranda cuspidifolia mart. (bignoniaceae) as antibacterial agent.abstract this study evaluated, in vitro, the antimicrobial activity of the hexane extract (jche), methanol extract (jcme), and chloroform fraction (jccf) of bark from jacaranda cuspidifolia mart. (family bignoniaceae), a brazilian medicinal plant, traditionally used as anti-syphilis and anti-gonorrhea treatment. the antimicrobial activity was evaluated using the disc diffusion method followed by the determination of minimum inhibitory concentration (mic) values. jche was not active against the b ...201121663482
self-transmissibility of the integrative and conjugative element icepm1 between clinical isolates requires a functional integrase, relaxase, and type iv secretion system.integrative and conjugative elements (ices), which are chromosomal mobile elements, can conjugatively transfer between bacteria. recently, we identified a genomic island of proteus mirabilis, a common agent of catheter-associated urinary tract infection (uti), that possesses all the properties consistent with an ice. this element, designated icepm1, is highly conserved in other causative agents of uti, suggesting its mobility. we demonstrate that icepm1 can actively excise from the chromosome in ...201121665966
anti-proliferative activity of meroditerpenoids isolated from the brown alga stypopodium flabelliforme against several cancer cell lines.the sea constitutes one of the most promising sources of novel compounds with potential application in human therapeutics. in particular, algae have proved to be an interesting source of new bioactive compounds. in this work, six meroditerpenoids (epitaondiol, epitaondiol diacetate, epitaondiol monoacetate, stypotriol triacetate, 14-ketostypodiol diacetate and stypodiol) isolated from the brown alga stypopodium flabelliforme were tested for their cell proliferation inhibitory activity in five ce ...201121673894
lateral sinus thrombosis in children: a review.a retrospective study was undertaken to review the clinical presentation, evaluation, management, and outcome of otogenic lateral sinus thrombosis (lst) in children. all pediatric patients with lst seen in our department between 1999 and 2007 were included; there were 9 cases involving 6 boys and 3 girls whose ages ranged from 8 to 12 years. they had all been treated with antibiotics elsewhere prior to admission, and the duration of symptoms before admission ranged from 5 to 18 days. the most co ...201121674459
effect of curcumin against proteus mirabilis during crystallization of struvite from artificial urine.we investigated the activity of curcumin against proteus mirabilis and the struvite crystallization in relation to urinary stones formation. in order to evaluate an activity of curcumin we performed an in vitro experiment of struvite growth from artificial urine. the crystallization process was induced by proteus mirabilis to mimic the real urinary tract infection, which usually leads to urinary stone formation. the results demonstrate that curcumin exhibits the effect against proteus mirabilis ...201221808656
[urinary tract infection in patient after brain injury].intruduction: one of the most important problems in treating patients after head injury is urinary complication. the first place take urinary infections, which disturb rehabilitation process. clean intermittent catherization, bladder training and good care do not preserve infection. the purpose of our study was to present the frequency of urinary infections and main pathogens involved in these infections among patients treated in the department of rehabilitation after head injury.201121812229
a computer-assisted diagnostic and treatment concept to increase accuracy and safety in the extracranial correction of cranial vault asymmetries.purpose: proteus syndrome is described as a progressive, asymmetric, disproportional overgrowth of various parts of the body. the theory of somatic mosaicism is widely accepted to be the cause of this disease. affected patients present very heterogeneous symptoms, but in about 30% craniofacial deformities are the leading clinical features. because no causal therapy exists, treatment options are limited to surgical improvement of functional constraints. materials and methods: a computer-assisted ...201121807449
proteus syndrome: a medical rarity. 201121804811
synthesis, characterization, theoretical crystal structure, and antibacterial activities of some transition metal complexes of the thiosemicarbazone (z)-2-(pyrrolidin-2-ylidene)hydrazinecarbothioamide.problem statement. in iraq like most third world countries, attempts discovered new antibiotic drugs derived from thiosemicarbazide and its metal complexes and developed the branch of applied in organic chemistry. approach. new (z)-2-(pyrrolidin-2-ylidene)hydrazinecarbothioamide (l) was synthesized in a good yield by the reaction of pyrrolidone with thiosemicarbazide. co(ii), ni(ii), and cu(ii) complexes of (l) were prepared and characterized by ft-ir, uv/visible spectra, (1)hnmr, and chn analys ...201121804771
giant chondrolipoma of the tongue in a 14-year-old child with mandibular hypertrophy. isolated lesion or regional overgrowth syndrome.chondrolipoma is a rare condition; especially in the oral cavity. the authors described a giant chondrolipoma of the tongue, associated with mandibular and lower lip overgrowth, in a 14-year-old boy. after tumour excision, histopathological examination showed mature cartilage within lobules of mature adipocytes. this is the first case of giant chondrolipoma associated with facial overgrowth. the aetiology and the association with a localized proteus syndrome are discussed.201121802258
in vitro antimicrobial and antioxidant activity of acetone and methanol extracts from thymus leucotrichius (lamiaceae).thymus species has been used as tonic and herbal tea, antiseptic, antitussive, carminative, antioxidant, anti-inflammatory and antimicrobial activities. the acetone and methanol extracts of thymus (t.) leucotrichius (labiatae/lamiaceae) was examined for antimicrobial and antioxidant properties.201121796869
detection of the staphylococcal multiresistance gene cfr in proteus vulgaris of food animal origin.objectives to investigate the presence and the genetic environment of the multiresistance gene cfr in naturally occurring gram-negative bacteria of pigs. methods a total of 391 bacterial isolates with florfenicol mics of =16 mg/l, obtained from 557 nasal swabs of individual pigs, were screened by pcr for the known florfenicol resistance genes. the species assignment of the cfr-carrying isolate was based on the results of gram's staining, colony morphology, 16s rdna sequencing and biochemical pro ...201121795256
synthesis of novel fluconazoliums and their evaluation for antibacterial and antifungal activities.a series of novel fluconazoliums were synthesized and their bioactive evaluation as potential antibacterial and antifungal agents were described. some target compounds displayed good and broad-spectrum antimicrobial activities with low mic values ranging from 0.25 to 64 µg/ml against all the tested strains, including three gram-positive bacteria (staphylococcus aureus, mrsa and bacillus subtilis), three gram-negative bacteria (escherichia coli, pseudomonas aeruginosa and bacillus proteus) as wel ...201121794961
synthesis and studies of novel 2-(4-cyano-3-trifluoromethylphenyl amino)-4-(quinoline-4-yloxy)-6-(piperazinyl/piperidinyl)-s-triazines as potential antimicrobial, antimycobacterial and anticancer agents.a series of novel s-triazine analogs were synthesized and characterized by ir, (1)h nmr, (13)c nmr, (19)f nmr spectroscopy and elemental analysis. preliminary screening of target compounds against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberculosis ...201121794959
a mosaic activating mutation in akt1 associated with the proteus syndrome.background the proteus syndrome is characterized by the overgrowth of skin, connective tissue, brain, and other tissues. it has been hypothesized that the syndrome is caused by somatic mosaicism for a mutation that is lethal in the nonmosaic state. methods we performed exome sequencing of dna from biopsy samples obtained from patients with the proteus syndrome and compared the resultant dna sequences with those of unaffected tissues obtained from the same patients. we confirmed and extended an o ...201121793738
hamartoma syndromes, exome sequencing, and a protean puzzle.gross malformations have been well known for centuries, even millennia. however, it was not until 1904 that albrecht coined the concept of hamartoma. in 1934, the concept entered the field of developmental pathology generally in reference to tissue malformations. hamartomata are localized overgrowths of a single tissue or combination of tissues, indigenous to the affected body part or organ, usually growing at the same rate as the normal components and causing little pain or functional impairmen ...201121793737
new delhi metallo-ss-lactamase-1: detection and prevention. 201121788418
chemical composition and antimicrobial activity of the essential oil of rosemary.the composition of the essential oil of rosemary was analyzed by gas chromatography-mass spectrometry (gc-ms). 22 components, which constitute 97.41% of the oil, were identified. the major constituents were 1,8-cineole (26.54%) and a-pinene (20.14%). minimum inhibitory concentrations (mics), minimal bactericidal concentration (mbc) and time-kill dynamic processes against three gram-positive bacteria (staphylococcus epidermidis, staphylococcus aureus and bacillus subtilis), three gram-negative ba ...201121787731
new inhibitors of colony spreading in bacillus subtilis and bacillus anthracis.we have recently characterized sliding motility in bacillus subtilis strains that lack functional flagella, and here describe the discovery of inhibitors of colony spreading in these strains as well as the aflagellate pathogen, bacillus anthracis. aflagellate b. subtilis strains were used to screen for new types of antibacterials that might inhibit colony spreading on semi-solid media. from a diverse set of organic structures, p-nitrophenylglycerol (npg), an agent used primarily in clinical labo ...201121784632
[clinical and economic expedience of ertapenem therapy of complicated urinary tract infection].clinical and economic investigation of various antibiotics use in the treatment of complicated urinary tract infection (cuti) was performed under the russian economic environment. the drugs of comparison were ertapenem, ceftriaxone and levofloxacin. direct costs and their structure were shown, and the cost efficiency was calculated. alternative analysis and one-side susceptibility analysis were performed. in complicated urinary tract infections when the major pathogens were escherichia coli, kle ...201121780670
[proteus mirabilis isolated from urine, resistance to antibiotics and biofilm formation].in the rather rare urinary pathogen proteus mirabilis resistance to antibiotics and ability to form biofilm were studied.201121780025
antibacterial activity of selected ethnomedicinal plants from south india.to screen the antimicrobial potential of three ethnomedicinal plants chassalia curviflora thw. (c. curviflora), cyclea peltata hook. f. & thomson (c. peltata) and euphorbia hirta l (e. hirta) used in folk medicines in aarukani hills kani tribe, tamil nadu, india against human bacterial pathogens.201121771680
antibacterial activity of leaves and inter-nodal callus extracts of mentha arvensis l.to determine the anti-bacterial efficacy of chloroform, ethanol, ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from mentha arvensis (m. arvensis) against salmonella typhi(s. typhi), streptococcus pyogenes(s. pyogenes), proteus vulgaris(p. vulgaris) and bacillus subtilis(b. subtilis).201121771452
carbon nanoparticles-assisted mediator-less microbial fuel cells using proteus vulgaris.recently mediator-less microbial fuel cells (mfcs) are attracting great interest among researchers due to their potential applications to electricity generation as well as wastewater treatment. common mediator-less mfcs employ electroactive bacteria called exoelectrogens to directly transfer electrons to the anode from the bacteria. however, exoelectrogens are rather limited in number and thus may not find general use for practical purposes. here we showed our results in which mediator-less mfcs ...201121764289
comprehensive inhibitor profiling of the proteus mirabilis metalloprotease virulence factor zapa (mirabilysin).in this study we report for the first time the comprehensive inhibitor profiling of the proteus mirabilis metalloprotease virulence factor zapa (mirabilysin) using a 160 compound focused library of n-alpha mercaptoamide dipeptides, in order to map the s(1)(') and s(2)(') binding site preferences of this important enzyme. this study has revealed a preference for the aromatic residues tyrosine and tryptophan in p(1)(') and aliphatic residues in p(2)('). from this library, six compounds were identi ...201121762758
susceptibility of esbl-producing enterobacteriaceae with the new clsi breakpoints.in 2010 clsi lowered the susceptibility breakpoints of some cephalosporins and aztreonam for enterobacteriaceae and eliminated the need to perform esbl screening and confirmatory tests. the aim of this study was to determine how many esbl-producing strains will test susceptible for three common species of enterobacteriaceae using the new breakpoints. as determined with the clsi screening and confirmatory tests, 382 consecutive esbl-producing strains were collected at our hospital between 2007 an ...201121752977
prevalence of sxt/r391-like integrative and conjugative elements carrying blacmy-2 in proteus mirabilis.objectives to characterize the vectors involved in the dissemination of bla(cmy-2) genes in clinical isolates of proteus mirabilis collected between 1999 and 2007. methods plasmid analysis of 19 p. mirabilis carrying ampc genes was performed by pcr-based replicon typing, s1-pfge and southern hybridization with ampc and replicon probes. isolates that could not be characterized were examined for the presence of sxt/r391-like elements. to demonstrate the involvement of these elements in the dissemi ...201121752830
microbiology at first visit of moderate-to-severe diabetic foot infection with antimicrobial activity and a survey of quinolone monotherapy.samples from 1295 patients with diabetic foot infection were evaluated; 4332 samples were collected with an average of 3.3 samples per patient. fifty-seven percent of patients had a 2b ulcer and 23% had a 3b ulcer according to texas university classification. in 64.2% of samples collected at first visit an etiologic agent was identified. about 40% of the positive samples were polymicrobial. gram positive bacteria were more frequently isolated (52.6%), staphylococcus aureus was the most frequentl ...201121824673
microbiological profile with antibiotic sensitivity pattern of cholesteatomatous chronic suppurative otitis media among children.chronic suppurative otitis media (csom) is the most common cause of childhood hearing impairment in the developing countries and atticoantral type is associated with increased incidence of intracranial and extracranial complications. this study was undertaken to define the microbiology of atticoantral type of chronic otitis media and the antibiotic sensitivity pattern, thereby reducing the potential risks of complications.201121715027
composition and antimicrobial activity of achillea distans essential oil.the essential oil of achillea distans w. et k. flower heads was analyzed by gc and gc-ms. altogether 43 components in concentrations more than 0.1% were identified representing 93.5% of the oil composition. the main constituents were 1,8-cineole (16.8%), trans-thujone (9.8%), sabinene (8.2%), borneol (7.5%), beta-pinene (6.5%), and camphor (5.8%). the oil showed moderate activity against staphylococcus aureus and candida albicans, and weak activity against salmonella typhimurium, proteus vulgari ...201121815436
purple urine. 201121738253
studies of antimicrobial activities of some 4-thiazolidinone fused pyrimidines, [1,5]-benzodiazepines and their oxygen substituted hydroxylamine derivatives.thiazolidin-4-one fused pyrimidines, [1,5]-benzodiazepines and their oxygen substituted hydroxylamine derivatives have been screened for antibacterial, antifungal and antimalarial activity. bacillus subtilis, escherichia coli, proteus mirabilis and salmonella typhi were used for antibacterial screening. aspergillus fumigatus and candida albicans were used for antifungal screening and plasmodium species were used for antimalarial screening. the antibacterial and antifungal activities are expresse ...201021694993
antimicrobial activity of two essential oils.the aim of the study was to evaluate the antimicrobial activity of essential oils in vitro for possible application to reduce the content of microorganisms in the air of animal houses. the essential oils of cymbopogon citrarus l. and malaleuca alternifolia l. were screened against bacteria staphylococcus aureus, enterococcus faecium, pseudomonas aeruginosa, escherichia coli, proteus mirabilis and yeast candida albicans. the minimal inhibitory concentration of the active essential oils was tested ...201121739934
a new class of 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazine analogues with antimicrobial/antimycobacterial activity.this study presents the synthesis and in vitro pharmacological evaluations of novel 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazines. the title compounds were assayed for their in vitro antimicrobial activity against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria) and four fungi (aspergillus niger, aspergillus fumigatus, a ...201121740100
surface microbial consortia from livarot, a french smear-ripened cheese.the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ...201121815832
evaluation of efficacy of topical povidone-iodine and different types of fluoroquinolones in the sterilization of bacterial flora on the conjunctiva.abstract purpose: to compare the sterilization effectivity of topical povidone-iodine, ciprofloxacin, and ofloxacin on the conjunctival bacterial flora. methods: one hundred sixty-four eyes of 164 patients scheduled for cataract surgery were prospectively analyzed. conjunctival cultures were obtained 1 day before the surgery without any topical application in all patients. conjunctival flora was examined in 53 eyes of 53 patients (group i) 15ôçëmin after 5% topical povidone-iodine application on ...201121834670
antimicrobial resistance patterns of uropathogens among children in istanbul, turkey.urinary tract infections are a common cause of end-stage renal disease in turkey. this prospective study investigated the antibiotic resistance patterns of uropathogens in order to recommend appropriate therapeutic protocols for children with urinary tract infections in istanbul, turkey. between october 2007 and october 2008, children presenting with a first episode of urinary tract infection to a pediatric outpatient clinic were enrolled in the study. urine samples were cultured, and antimicrob ...201121710858
acidic environments induce differentiation of proteus mirabilis into swarmer morphotypes.although swarmer morphotypes of proteus mirabilis have long been considered to result from surfaced-induced differentiation, the present findings show that, in broth medium containing urea, acidic conditions transform some swimmer cells into elongated swarmer cells. this study has also demonstrates that p. mirabilis cells grown in acidic broth medium containing urea enhance virulence factors such as flagella production and cytotoxicity to human bladder carcinoma cell line t24, though no signific ...201121707738
[russian results of the international epidemiological study of clinical aspects and resistance to antimicrobial medicines of cystitis uropathogens in females (aresc): significance of empiric therapy].a total of 656 female patients from russia aged between 18 to 65 years with uncomplicated cystitis entered the international aresc trial, of them 647 were eligible for final analysis. positive cultural urine tests ( > 10(4) cfu/ml) were in 419 (64.7%) patients, 393 (93.8%) patients had monoinfection. the central laboratory of genoa (to which the isolated samples were sent) has tested 416 uropathogens from 399 patients. the following pathogens were isolated: e. coli (72.6%), enterococcus (7.0%), ...201121815455
detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (sift-ms).selected ion flow tube-mass spectrometry has been used to measure the volatile compounds occurring in the headspace of urine samples inoculated with common urinary tract infection (uti)-causing microbes escherichia coli, proteus vulgaris, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, enterococcus faecalis, or candida albicans. this technique has the potential to offer rapid and simple diagnosis of the causative agent of utis.201121741416
investigation of the antimicrobial activity of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.).the aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. material and methods. ethanol extract from the leaves of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.) was produced at the department of food technology, kaunas university of technology. the antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria s ...201121822040
Cerebriform Plantar Hyperplasia: The Clinico-pathological Hallmark of Proteus Syndrome. 201121681363
Macrodactyly with skin hypertrophy: a minimal form of the Proteus syndrome.The Proteus syndrome was described 1983 . It has asymmetric gigantism of the limbs, verrucous epidermal naevi, cerebriform enlargement of the plantar region, vascular malformations and neoplasms, as lipomas. It received this denomination after Proteus from the Greek mythology, who had the ability to change his form . A 15 year-old boy, reported a congenital hypertrophy with syndactily of the second and third right fingers . The second case is a 35 year-old man, who reported that since birth the ...201121738976
Trimethoprim/sulfametrole: evaluation of the available clinical and pharmacokinetic/pharmacodynamic evidence.Emergence of resistance to widely used trimethoprim/sulfamethoxazole (TMP/SMX) as well as common adverse events in human immunodeficiency virus (HIV)-infected patients casts interest on combinations of TMP with other sulfonamides. Sulfametrole (SMT) combined with TMP could provide a choice for difficult-to-treat infections, particularly when administered intravenously. The objective of this review was to evaluate the available clinical and pharmacokinetic/pharmacodynamic (PK/PD) evidence regardi ...201121741802
relationship between chemical structure and biological activity of alkali metal o-, m- and p-anisates. ft-ir and microbiological studies.in this work we investigated relationship between molecular structure of alkali metal o-, m-, p-anisate molecules and their antimicrobial activity. for this purpose ft-ir spectra for lithium, sodium, potassium, rubidium and caesium anisates in solid state and solution were recorded, assigned and analysed. microbial activity of studied compounds was tested against escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and proteus vulgaris. in order to evaluate the dependency between chem ...201121852187
the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds.the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ...201122182219
vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital.an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ...201121917010
Development of an antibiogram in a long-term care facility.To create an antibiogram-a profile of an organism's susceptibility/resistance to a panel of antibiotics- for a long-term care facility to assess the prevalence of resistance of bacteria present at the facility.201122079792
Antimicrobial susceptibility of Gram-negative organisms from intraabdominal infections and evolution of isolates with extended spectrum ß-lactamases in the SMART study in Spain (2002-2010).Introduction. The SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study records the antimicrobial susceptibility of Gram-negative bacilli obtain from intraabdominal infections with special focus in isolates with extended spectrum ß-lactamases (ESBLs). Material and Methods. The antimicrobial susceptibility of 8,869 isolates was analyzed by microdilution during the SMART study performed in Spain from 2002 to 2010. Isolates were recovered in 16 centres. Results. Escherichi ...201122173194
Origins of the elephant man: mosaic somatic mutations cause Proteus syndrome. 201122035467
[Synthesis and antimicrobial evaluation of coumarin-based benzotriazoles and their synergistic effects with chloromycin and fluconazole].A series of new coumarin-based benzotriazole derivatives were successfully synthesized via a multi-step sequence of cyclization, etherification and N-alkylation, and were confirmed by 1H NMR, IR, MS spectra as well as elemental analyses. All these synthesized coumarin compounds were evaluated for in vitro antimicrobial activities against four Gram-positive bacteria, four Gram-negative bacteria and three fungi by two fold serial dilution technique. The bioactive assay showed that all these prepar ...201122010349
potential role of proteus-providencia-morganella species in quality deterioration of beef.proteeae isolation medium of hawkey et al. (1986) was effective for isolation and enumeration of proteus-providencia-morganella spp. from beef and beef products. on this medium colonies were brown, surrounded by a zone of clearing. in an examination of retail beef samples packaged and stored in polyvinyl chloride (pvc) film, 5% contained these bacteria in a concentration of 10(1)-10(2) cells per gram or cm(2). isolates from retail beef samples included p. vulgaris, p. alcalifaciens, p. rettgeri, ...199322061226
nosocomial infection and characterization of extended-spectrum β-lactamases-producing enterobacteriaceae in northeast brazil.extended spectrum β-lactamases (esbls) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide.201121860889
macroscopic behavior of systems with an axial dynamic preferred direction.we present the derivation of the macroscopic equations for systems with an axial dynamic preferred direction. in addition to the usual hydrodynamic variables, we introduce the time derivative of the local preferred direction as a new variable and discuss its macroscopic consequences including new cross-coupling terms. such an approach is expected to be useful for a number of systems for which orientational degrees of freedom are important including, for example, the formation of dynamic macrosco ...201122120542
Acinetobacter baumannii is Able to Gain and Maintain a Plasmid Harbouring In35 Found in Enterobacteriaceae Isolates From Argentina.The aim of this study was to determine the presence of bla (CTX-M-2) in our A. baumannii population and their putative role as an alternative mechanism of resistance to third-generation cephalosporins in this species. The bla (CTX-M-2) gene is widespread among the Enterobacteriaceae isolates from our country; however, it was not found in 76 isolates A. baumannii non-epidemiologically related clinical isolates resistant to third-generation cephalosporins ...201122119898
biogenic amines produced by enterobacteriaceae isolated from meat products.biogenic amines in ground meat and processed meat products are one of the indicators to determine the poor quality raw material. major histamine forming bacterium was escherichia coli (strain ec04 with 65.88 mg/100 ml in brain heart infusion medium), followed by the microorganisms morganella morganii (strain mm4 with 8.40 mg/100 ml and strain mm7 with 8.28 mg/100 ml) and proteus mirabilis (strain pm02 with 8.76 mg/100 ml), respectively. the highest putrescine production level was found in citrob ...200122062111
role of bacteria isolates in the spoilage of fermented african oil bean seed ugba.study was carried out to determine the spoilage association of traditionally fermented ugba, product of african oil bean seed. samples were collected from three markets within enugu metropolis in eastern nigeria. microbial population of ugba was isolated, characterized and identified. effect of time on product quality, total viable cell count and individual growth pattern of isolates was evaluated. microorganisms isolated were proteus sp., klebsiella sp., staphylococcus epidermidis, e. coli, bac ...201021848074
Plasmid-mediated transfer of the bla(NDM-1) gene in Gram-negative rods.The latest threat of multidrug-resistant Gram-negative bacteria corresponds to the emergence of carbapenemase NDM-1 (New Delhi metallo-ß-lactamase) producers, mostly in Enterobacteriacae. Five bla(NDM) (-1) -positive plasmids of different incompatibility groups (IncL/M, FII, A/C and two untypeable plasmids) from clinical Enterobacteriaceae were evaluated for conjugation properties and host specificity. Successful conjugative transfers were obtained using all tested enterobacterial species as rec ...201122092811
clinical characteristics and outcomes of patients with extended-spectrum β-lactamase-producing bacteremias in the emergency department.extended-spectrum β-lactamase (esbl)-producing bacteria have been spreading from hospitals to communities. despite this, there are limited emergency department (ed) patient-based studies about these bacteremias. a retrospective matched case-control study with a ratio of 1:3 was conducted at a university hospital. the case group consisted of patients aged >16 years with esbl-producing bacteremias in the ed. patients matched for age and sex with non-esbl-producing bacteremias were sampled as the c ...201122033790
Bactericidal properties of pradofloxacin against veterinary pathogens.Pradofloxacin is a new veterinary 8-cyano-fluoroquinolone developed for use against bacterial infections in dogs and cats involving both aerobic and anaerobic bacteria. The minimal bactericidal concentrations have been determined against clinical isolates of Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus canis, Proteus spp., Fusobacterium spp., Porphyromonas gingivalis and Prevotella species. A subset of these species was selected, ...201122209121
effect of starter culture and storage temperature on the content of biogenic amines in dry fermented sausage polican.water activity, ph, microbial counts (total counts/tcm/, coliforms, enterobacter, proteus, pseudomonas, escherichia and lactobacillus spp., respectively), and seven biogenic amines (ba) were determined in dry fermented sausage 'polican' produced using starter culture a (lactobacillus sakei, staphylococcus carnosus, s. xylosus) or b (l. sakei, s. carnosus, pediococcus pentosaceus), ripened 42 days, and subsequently stored at 8 or 22°c 60 days. counts of lactobacilli were higher and tcm lower in t ...200122062781
Effective cohorting and "superisolation" in a single intensive care unit in response to an outbreak of diverse multi-drug-resistant organisms.Cohorting patients in dedicated hospital wards or wings during infection outbreaks reduces transmission of organisms, yet frequently, this may not be feasible because of inadequate capacity, especially in the intensive care unit (ICU). We hypothesized that cohorting isolation patients in one geographic location in a single ICU and using enhanced isolation procedures ("superisolation") can prevent the further spread of highly multi-drug-resistant organisms (MDRO).201121936667
control of clinical pathogens by the haemolymph of paratelphusa hydrodromous, a freshwater crab.in the present study, effort has been made to find the antimicrobial activity of haemolymph collected from freshwater crab, paratelphusa hydrodromous. the haemolymph collected was tested for antimicrobial assay by disc diffusion method against clinical pathogens. five bacterial species, namely, escherichia coli, klebsiella pneumonia, proteus mirabilis, pseudomonas aeruginosa, staphylococcus aureus, and five fungal strains, namely and aspergillus flavus, aspergillus fumigatus, aspergillus niger, ...201122084719
Antibacterial activity of some Indian Ayurvedic preparations against enteric bacterial pathogens.In Ayurveda, various herbal preparations are clinically used to prevent or cure infectious diseases. Herbal preparations such as Triphala churna, Hareetaki churna, Dashmula churna, Manjistadi churna, Sukhsarak churna, Ajmodadi churna, Shivkshar pachan churna, Mahasudarshan churna, Swadist Virechan churna and Pipramool churna were investigated by preparing their organic solvent extract for antibacterial potential against enteric bacterial pathogens such as Escherichia coli, Staphylococcus aureus, ...201122171288
Bacterial infections of Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to gamete collecting weirs in Michigan.Herein, we describe the prevalence of bacterial infections in Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to spawn in two tributaries within the Lake Michigan watershed. Ten bacterial genera, including Renibacterium, Aeromonas, Carnobacterium, Serratia, Proteus, Pseudomonas, Hafnia, Salmonella, Shewanella and Morganella, were detected in the kidneys of Chinook salmon (n = 480) using culture, serological and molecular analyses. Among these, Aeromonas salmonicida was detected at ...201222168454
Childhood suppurative otitis media in Abakaliki: isolated microbes and in vitro antibiotic sensitivity pattern.Suppurative otitis media (SOM) is the most common pediatric problem seen by otolaryngologists in Nigeria. Devising simple and effective ways of treating pediatric patients with suppurating ears, especially in situations without a specialist care, will help prevent chronicity. Our experience with som at the ebonyi state university teaching hospital (ebsuth), abakaliki, is reviewed in this study. it may serve as a guide in patient care.201121860131
[Uncomplicated lower urinary tract infections in females--clinical aspects, aetiology and antimicrobial resistance epidemiology. Results of the ARESC (Antimicrobial Resistance Epidemiological Survey on Cystitis) study in Poland and their implications for empiric therapy].The Polish results of the international ARESC study are reported concerning clinical aspects, epidemiology, and antimicrobial susceptibility of uropathogens.201121913487
Fluorinated s-triazinyl piperazines as antimicrobial agents.A series of 1,3,5-triazine derivatives that contain 4-amino-2-trifluoromethyl-benzonitrile, 8-hydroxyquinoline, and different piperazines as substituents at the carbon atoms of the triazine ring have been synthesized by a simple and efficient synthetic protocol. The chemical structures of the compounds were elucidated with the aid of IR, 1H NMR and 13C NMR spectroscopy, and elemental analysis. The antimicrobial activity of the compounds was tested against seven bacteria (Staphylococcus aureus MT ...201121950158
Facial infiltrating lipomatosis: A case report and review of literature.Infiltrating lipomatosis of the face has been described as a congenital disorder in which mature lipocytes invade adjacent tissues in the facial region. The presentation is always unilateral with hypertrophy of hard and soft structures on the affected side of the face. We present a case of a 27-year-old female who reported with a complaint of recurrent unilateral facial swelling with history of two previous resections, the histopathology or details of these surgeries were not available. The pati ...201122096727
Molecular mimicry between spermatozoa and bacteria.Sperm immobilization factor isolated from Staphylococcus aureus immobilizes human spermatozoa as well as motile bacteria, showing that sperm immobilization factor receptor might be shared by bacteria and human spermatozoa. Thus, we sought to identify a common sperm immobilization factor binding receptor on spermatozoa and bacteria.201122019166
frequency of extended spectrum beta lactamase producing enterobacteriaceae among urinary pathogen isolates.objective: to determine the frequency of extended-spectrum beta lactamase (esbl) producing enterobacteriaceae in urinary isolates. study design: observational study. place and duration of study: ziauddin university hospital, karachi, from february to october 2008. methodology: all members of enterobacteriaceae isolated from urinary samples of in-patients were included and identified using standard biochemical tests. urinary samples from out-patients were excluded. detection of esbl was carried o ...201122166694
development of 3d architecture of uropathogenic proteus mirabilis batch culture biofilms-a quantitative confocal microscopy approach.this work studies the development of the 3d architecture of batch culture p. mirabilis biofilms on the basis of morpho-topological descriptors calculated from confocal laser scanning microscopy (clsm) stacks with image processing routines. a precise architectonical understanding of biofilm organization on a morpho-topological level is necessary to understand emergent interactions with the environment and the appearance of functionally different progeny swarmer cells. p. mirabilis biofilms were g ...201121864585
phenolic compounds and biological activity of kitaibelia vitifolia.this study was aimed at evaluating the antioxidant activity and efficacy of the ethanolic extract of the endemic plant species kitaibelia vitifolia in inhibiting the growth of selected fungi and bacteria. antimicrobial activity was tested using the broth dilution procedure for determination of minimum inhibitory concentration (mic). mics were determined for eight selected indicator strains. the highest susceptibility to k. vitifolia ethanolic extract among the bacteria tested was exhibited by ba ...201121877950
[Influence of predominant aerobic bacteria isolated from different healthy animals on daidzein biotransforming capacity by co-culture with different daidzein biotransforming bacteria].To investigate the influence of isolated predominant aerobic bacteria on daidzein biotransformation capacity by co-culture with daidzein biotransforming bacteria.201122097769
asymmetric, disproportionate craniofacial hyperostoses of mosaic pattern in a patient not meeting proteus syndrome criteria.a 34-year-old patient with asymmetric hyperostosis of the craniofacial skeleton much more pronounced on the right side is presented. a long-term follow-up of 16 years showed progression of overgrowth even after skeletal maturity and despite repeated surgical corrections focusing on regional reduction of the hyperostoses. clinical situation during infancy, adolescence, and adulthood is documented. although the craniofacial hyperostosis in the patient was indicative of the proteus syndrome, the ap ...201122134251
antibacterial activity of water-soluble extract from pine needles of cedrus deodara.the antibacterial activity of water-soluble extract from pine needles of cedrus deodara (wec) was evaluated on five food-borne bacteria, and its related mechanism was investigated by transmission electron microscope. in vitro antibacterial assay showed that wec possesses a remarkable antibacterial activity against tested food-borne bacteria including escherichia coli, proteus vulgaris, staphylococcus aureus, bacillus subtilis and bacillus cereus, with the minimum inhibitory concentration (mic) a ...201122104118
synthesis and biological evaluation of some thiazolidinones as antimicrobial agents.a novel series of thiazolidinone derivatives namely 4-(4-dimethylamino-6-{4-[5-(4-ethylpiperazin-1-ylmethyl)-4-oxo-2-phenylthiazolidin-3-yl]-phenylamino}-[1,3,5]triazin-2-yloxy)-1-methyl-1h-quinolin-2-one have been synthesized from the key intermediate 4-[4-(4-aminophenylamino)-6-dimethylamino-[1,3,5]triazin-2-yloxy]-1-methyl-1h-quinolin-2-one (7). condensation reaction of compound 7 with different aldehyde derivatives were performed to obtain schiff base derivatives, which after cyclization gav ...201122182927
[the susceptibility of proteus mirabilis strains isolated from white stork (ciconia ciconia)].proteus sp. rods are ubiquitous bacteria, widespread in the environment and classified also as opportunistic human pathogens. the aim of our study was to evaluate susceptibility of proteus mirabilis strains isolated from white stork (ciconia ciconia) regarding as his natural bacterial flora, compare and discuss their results with data obtained from scientific literature for clinical strains of the same species. susceptibility of 59 p. mirabilis strains was estimated for 27 antimicrobials using d ...201122184908
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