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an appraisal of current management of childhood urinary tract infections among private medical practitioners.private medical practitioners manage substantial number of children with urinary tract infection in nigeria. it is important to evaluate the management of urinary tract infection (uti), because of complications that may arise form improper treatment.200919485147
dependence of swarming in escherichia coli k-12 on spermidine and the spermidine importer.in a previous work, it was observed that the swarming of polyamine-deficient proteus mirabilis (speb::sm) was severely inhibited on luria-bertani (lb) swarming plates (lbsw) (lb, 0.5% glucose, 0.5% agar), and it was clarified that extracellular putrescine was important as a signaling molecule for the induction of swarming in p. mirabilis. however, a polyamine-deficient strain (delta-speab delta-spec) of escherichia coli swarmed as well as the parental strain on lbsw plates. we report that the sw ...200919493013
structural and functional studies of truncated hemolysin a from proteus mirabilis.in this study we analyzed the structure and function of a truncated form of hemolysin a (hpma265) from proteus mirabilis using a series of functional and structural studies. hemolysin a belongs to the two-partner secretion pathway. the two-partner secretion pathway has been identified as the most common protein secretion pathway among gram-negative bacteria. currently, the mechanism of action for the two-partner hemolysin members is not fully understood. in this study, hemolysis experiments reve ...200919494116
oral bacterial flora of the chinese cobra (naja atra) and bamboo pit viper (trimeresurus albolabris) in hong kong sar, china.to determine the oral bacterial flora associated with two common local venomous snakes in hong kong, namely the chinese cobra (naja atra) and the bamboo pit viper (trimeresurus albolabris).200919494373
[thoraco-brachial cystic lymphangioma and proteus syndrome: prenatal diagnosis and mr follow-up]. 200919503050
preparation, physicochemical characterization and biological evaluation of cefodizime metal ion complexes.cefodizime is a broad spectrum cephalosporin belonging to the third generation agents. in this study, attention has been paid to the preparation, physicochemical characterization and biological evaluation of new cu2+, zn2+, fe3+, co2+ and al3+ complexes of cefodizime.200919505365
the aresc study: an international survey on the antimicrobial resistance of pathogens involved in uncomplicated urinary tract infections.the aresc (antimicrobial resistance epidemiological survey on cystitis) study is an international survey to investigate the prevalence and susceptibility of pathogens causing cystitis. female patients (n=4264) aged 18-65 years with symptoms of uncomplicated cystitis were consecutively enrolled in nine european countries as well as brazil during 2003-2006. pathogens were identified and their susceptibility to nine antimicrobials was determined. escherichia coli accounted for 76.7% of isolates. am ...200919505803
are sinus-track cultures reliable for identifying the causative agent in chronic osteomyelitis?the infection of bone that contains bone marrow called osteomyelitis, and is caused by different microorganisms. in this study, we aimed to determine the diagnostic value and accuracy of cultures of material from a sinus track compared with those of cultures of bone specimens that have been controversial.200919513734
[evaluation of effectiveness and tolerance of treatment with azithromycin 1.5% eye drops in bacterial conjunctivitis].azithromycin is a macrolide class antibiotic, recently adapted for topical use in ophthalmology. it is effective against the most frequent pathogens found in bacterial conjunctivitis, gram positive and gram negative bacteria. azithromycin has the specificity to have sustained high tissue levels: after repeated instillation, it has been shown to reach sustained concentrations above the mics of susceptible bacteria for 4 days in tears and for 7 days in conjunctiva.200919517846
evaluation of antibacterial activity, phenol and flavonoid contents of thespesia populnea flower extracts.flavonoids which were reported as having many pharmacological activities, antimicrobial, antioxidant, cytotoxic, chemoprevention activities and they possess strong antiproliferative effects related to inhibition of cell cycle progression and apoptosis induction. on the basis of this thespesia populnea (l.) sol. ex correa (family-malvaceae) was selected and it is having the major composition of flavonoids and the antibacterial activity of methanolic extract of thespesia populnea flowers was inves ...200919553175
crystalline bacterial biofilm formation on urinary catheters by urease-producing urinary tract pathogens: a simple method of control.the problem of catheter encrustation stems from infection by urease-producing bacteria. these organisms generate ammonia from urea, elevate the ph of urine and cause crystals of calcium and magnesium phosphates to form in the urine and the biofilm that develops on the catheter. in this study, a laboratory model was used to compare the ability of 12 urease-positive species of urinary tract pathogens to encrust and block catheters. proteus mirabilis, proteus vulgaris and providencia rettgeri were ...200919556373
epidemiology of antimicrobial resistance among gram-negative organisms recovered from patients in a multistate network of long-term care facilities.we identified 1,805 gram-negative organisms in cultures of urine samples obtained over a 10-month period from residents of 63 long-term care facilities. the prevalence of fluoroquinolone resistance in escherichia coli was 51% (446 of 874 isolates), whereas the prevalences of ceftazidime and imipenem resistance in klebsiella species were 26% and 6% (84 and 19 of 323 isolates), respectively. the prevalence of resistance varied significantly by facility type, size, and geographic location.200919566445
antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy.this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ...200919567344
pediatric chest wall and breast deformities.pediatric chest wall and breast deformities present as a wide spectrum of anomalies, and often occur coincidentally. chest wall abnormalities fall into two categories, congenital (which are largely hypoplastic) and deformational (including both chest wall malignancies and postoperative abnormalities). breast abnormalities can be categorized into three groups, including hypoplastic, hyperplastic, and deformational anomalies. hypoplastic breast anomalies require reconstruction with augmentation te ...200919568138
encephalocraniocutaneous lipomatosis.encephalocraniocutaneous lipomatosis (eccl) is a sporadically occurring neurocutaneous disorder of unknown aetiology. it has repeatedly been discussed as a localised form of proteus syndrome. in 2006, the first large series of patients was reported, and diagnostic criteria were proposed.200919574261
foot or hand malformations related to deep venous system anomalies of the lower limb in klippel-trénaunay syndrome.klippel-trénaunay syndrome (kts) is a capillary-lymphatic-venous malformation associated with soft tissue and skeletal hypertrophy of one or more limbs. deep venous system (dvs) anomalies are reported to be present in 8% to 18% of patients with kts; approximately 25% of patients with kts have hand or foot malformations.200919577333
serological characterization of the core region of lipopolysaccharides of rough proteus sp. strains.both smooth and rough proteus sp. strains can be found. the latter are characterized by their lack of an o-polysaccharide chain in the lipopolysaccharide (lps) molecule, which makes them suitable for obtaining anti-core sera. using this kind of material enables identifying fragments of the proteus lps core region that might be involved in cross-reactions. to date only a few similar epitopes have been established for the genus proteus.200919578814
endophthalmitis caused by proteus species: antibiotic sensitivities and visual acuity outcomes.the purpose of this study was to report the clinical presentation, causative organisms, antibiotic sensitivities, management, and visual acuity outcomes in patients with endophthalmitis caused by proteus species at a university teaching hospital over a 24-year period.200919584659
prevalence of ctx-m beta-lactamases in philadelphia, pennsylvania.ctx-m beta-lactamases were thought to be rare in the united states, but a recent study in texas showed that up to 70% of extended-spectrum beta-lactamase (esbl)-containing members of the enterobacteriaceae family were ctx-m positive (j. s. lewis, m. herrera, b. wickes, j. e. patterson, and j. h. jorgensen, antimicrob. agents chemother. 51:4015-4021, 2007). we used pcr to detect ctx-m in all 291 extended-spectrum cephalosporin-resistant gram-negative bacteria isolated in our laboratory during 200 ...200919587301
[early use of vacuum sealing drainage to repair the wound of the injured in wenchuan earthquake].to investigate the method of using vacuum sealing drainage (vsd) technique to repair the wound of the injured in wenchuan earthquake and its therapeutic effect.200919594008
excision of a large abdominal wall lipoma improved bowel passage in a proteus syndrome patient.proteus syndrome is an extremely rare congenital disorder that produces multifocal overgrowth of tissue. this report presents a surgical case of a large lipoma in the abdominal wall of a patient with proteus syndrome. she was diagnosed with proteus syndrome based on certain diagnostic criteria. the neoplasm increased in size gradually, producing hemihypertrophy of her left lower extremity and trunk, and spread to her retroperitoneum and her left abdominal wall. she experienced gradually progress ...200919598310
antimicrobial therapy for water-associated wound infections in a disaster setting: gram-negative bacilli in an aquatic environment and lessons from banda aceh.as members of the japan disaster relief (jdr) team in banda aceh, three of the authors treated 1,891 patients following the tsunami of 2004. of the 367 cases with traumatic injuries, 216 cases required antimicrobial therapy. the medical services were continued by the japan self-defense (jsd) medical team until mid-march 2005. of the 216 cases initially treated by jdr, 54 required prolonged antimicrobial therapy for persistent symptoms despite repeated debridement. the aim of this study is to rec ...200919618353
use of quorum sensing antagonists to deter the formation of crystalline proteus mirabilis biofilms.proteus mirabilis biofilms are a major cause of urinary catheter blockage. antibiotic-impregnated catheters used to prevent catheter blockage have achieved limited success. research has examined the efficacy of quorum sensing inhibitors against pseudomonas aeruginosa biofilms, but there are few reports of the effects of these compounds against crystalline p. mirabilis biofilms. this study examined the effect of two quorum sensing antagonists, p-nitrophenyl glycerol (pnpg) and tannic acid, agains ...200919619987
[multicenter study on the monitoring of in vitro susceptibility to tigeeyeline in santiago, chile].the objective of this multicenter study was to determine tigecycline susceptibility rates, measured by agar diffusion, in nine hospitals in santiago and to compare these rates with other antimicrobials. each center studied 20 strains per month. all intermediate and fully resistant strains as well as 10% of susceptibile strains were also studied by the broth microdilution method. overall, 2301 strains were studied displaying the following susceptibility rates for tigecycline: 100% for streptococc ...200919621154
a multinational survey of risk factors for infection with extended-spectrum beta-lactamase-producing enterobacteriaceae in nonhospitalized patients.infections caused by extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae are increasing in frequency and are associated with high mortality rates. circulation of ctx-m-type esbls in the community is of particular concern, because it may confound standard infection-control measures.200919622043
pattern of aerobic bacteria with antimicrobial susceptibility causing community acquired urinary tract infection.since antibiotic resistance among uropathogens have gradually been rising, so it is important to have knowledge about the pattern and antimicrobial susceptibility to choose the correct treatment regimen. a cross sectional study was carried out in the department of microbiology, mymensingh medical college between july 2007 to june 2008 to determine the prevalence, relationship between pyuria and urine culture and antibiotic resistance pattern among the bacterial isolates of community acquired uti ...200919623138
[isolation and study of perspective probiotic strain of spore-forming bacteria from bacillus genus].to study physiologic, biochemical as well as antagonistic characteristics of isolated strain of bacillus pumilus in comparison with known spore-forming bacteria from b. subtilis species.200919623717
[etiology of acute enteric infection in children with arid zone and effect of probiotics].to study etiologic structure of acute enteric infections (aei) with isolation of hemolytic and enteropathogenic escherichia and to assess the efficacy of probiotic therapy during infections caused by e. coli.200919623719
[rapid detection of salmonella in food by using fluorescently labeled phage o-i].to develop a rapid detection method for salmonella in food by using specific salmonella-phage o-i.200919623962
[ability of opportunistic enterobacteria to adapt to different temperatures].to study variability of enzymatic apparatus of opportunistic enterobacteria.200919459471
antimicrobial activity of selected plant species from "the argentine puna" against sensitive and multi-resistant bacteria.the plant species reported here are traditionally used in the "puna" or "altiplano" of argentina for ailments related to bacterial infections. the aim of this study was to evaluate their antimicrobial properties against a panel of sensitive and multi-resistant gram-positive and gram-negative bacteria.200919467313
the effect of edta instillations on the rate of development of encrustation and biofilms in foley catheters.the aim of this research was to examine whether a daily instillation of tetra sodium ethylenediaminetetraacetic acid (edta) solution could reduce the rate at which encrustation by crystalline proteus mirabilis biofilms blocks urinary catheters. sets of three bladder models were fitted with size 14 all-silicone catheters. tetra sodium edta solution was instilled into the catheter following biofilm development. catheters were examined by digital photography and scanning electron microscopy for evi ...200919468723
comparison of clinical symptoms scored according to the national institutes of health chronic prostatitis symptoms index and assessment of antimicrobial treatment in patients with chronic prostatitis syndrome.we examined a total of 194 patients over 18 years of age with chronic prostatitis syndrome and no evidence of structural or functional lower genitourinary tract abnormalities. the following data were obtained for each patient: clinical history--the severity of chronic prostatitis symptoms scored by a croatian translation of the nih cpsi questionnaire, clinical status including digitorectal examination, urethral swab specimens, and selective samples of urine and expressed prostatic secretion, acc ...200919423471
bacterial toxin higb associates with ribosomes and mediates translation-dependent mrna cleavage at a-rich sites.most pathogenic proteus species are primarily associated with urinary tract infections, especially in persons with indwelling catheters or functional/anatomic abnormalities of the urinary tract. urinary tract infections caused by proteus vulgaris typically form biofilms and are resistant to commonly used antibiotics. the rts1 conjugative plasmid from a clinical isolate of p. vulgaris carries over 300 predicted open reading frames, including antibiotic resistance genes. the maintenance of the rts ...200919423702
effects of proteus vulgaris growth on the establishment of a cheese microbial community and on the production of volatile aroma compounds in a model cheese.to investigate the impact of proteus vulgaris growth on a multispecies ecosystem and on volatile aroma compound production during cheese ripening.200919426267
correlation between antibiotic consumption and bacterial resistance as quality indicator of proper use of these drugs in inpatients.antibiotics are the most frequently used medications in serbian hospitalized patients. information about antibiotic utilization and sensitivity among inpatients in serbia is scanty, and there are no available publications on the topic. the aim of this study was to investigate the correlation between antibiotic use and bacterial resistance in the clinical center nis, one of the biggest hospitals in serbia.200919432297
piscidin 4, a novel member of the piscidin family of antimicrobial peptides.piscidins are linear, amphipathic, antimicrobial peptides (amps) with broad, potent, activity spectrum. piscidins and other members of the piscidin family appear to comprise the most common group of amps in teleost fish. all piscidins and related members of the piscidin family described to date are 18-26 amino acids long.we report here the isolation of a novel 5329.25 da, 44-residue (ffrhlfrgakaifrgarqgxrahkvvsryrnrdvpetdnnqeep) antimicrobial peptide from hybrid striped bass (morone chrysops fem ...200919266617
large scale multiplex pcr improves pathogen detection by dna microarrays.medium density dna microchips that carry a collection of probes for a broad spectrum of pathogens, have the potential to be powerful tools for simultaneous species identification, detection of virulence factors and antimicrobial resistance determinants. however, their widespread use in microbiological diagnostics is limited by the problem of low pathogen numbers in clinical specimens revealing relatively low amounts of pathogen dna.200919121223
needle puncture and transcutaneous bone biopsy cultures are inconsistent in patients with diabetes and suspected osteomyelitis of the foot.needle puncture has been suggested as a method for identifying bacteria in the bones in patients with diabetes with osteomyelitis of the foot. however, no studies have compared needle puncture with concomitant transcutaneous bone biopsy, which is the current standard recommended in international guidelines.200919228109
strongyloides stercoralis hyperinfection presenting as acute respiratory failure and gram-negative sepsis in a patient with astrocytoma.in developing countries, strongyloides stercoralis infection is a common cause of morbidity and mortality. death from strongyloidosis can result from hyperinfection or disseminated disease. infections due to s. stercoralis are unusual in saudi arabia and are usually diagnosed in immigrants from endemic areas. we report a case in which s. stercoralis was isolated from the sputum of a patient with gram-negative sepsis and respiratory failure, and review the salient features of this disease. a high ...200919231269
otogenic cerebellar abscess: a case report.this case report describes the gradual deterioration of a healthy, highly functioning man who initially presented with a draining right ear. the patient's indolent neurologic decline and referral to an otologist ultimately led to the diagnosis and treatment of an otogenic cerebellar abscess, an increasingly rare intracranial complication of otitis media. we report this case to illustrate that severe complications of chronic otitis media still occur in the united states, to stress the importance ...200919358116
proteus syndrome: report of a case with recurrent abdominal lipomatosis.proteus syndrome (ps) is an extremely rare congenital hamartomatous syndrome that was first delineated by cohen and hayden (1). the estimated prevalence is less than 1 per 1,000,000 live births (2). it is a sporadic disorder that causes overgrowth of multiple tissues, especially bone, fat, and other connective tissues in a patchy or mosaic pattern. subcutaneous as well as internal lipomas that may grow to an enormous size are frequently observed. nevertheless, among the internal lipomas, abdomin ...200919361616
[phosphatase activity in amoeba proteus at low ph].in free-living amoeba proteus (strain b), three forms of tartrate-sensitive phosphatase were revealed using page of the supernatant of ameba homogenates obtained with 1% triton x-100 or distilled water and subsequent staining of gels with 2-naphthyl phosphate as substrate (ph 4.0). the form with the highest mobility in the ameba supernatant was sensitive to all tested phosphatase activity modulators. two other forms with the lower mobilities were completely or significantly inactivated not only ...200919370987
the high-affinity phosphate transporter pst in proteus mirabilis hi4320 and its importance in biofilm formation.proteus mirabilis causes urinary tract infections (utis) in individuals requiring long-term indwelling catheterization. the pathogenesis of this uropathogen is mediated by a number of virulence factors and the formation of crystalline biofilms. in addition, micro-organisms have evolved complex systems for the acquisition of nutrients, including the phosphate-specific transport system, which has been shown to be important in biofilm formation and pathogenesis. a functional pst system is important ...200919372157
organisms associated with ventilator associated pneumonia in intensive care unit.it is a cross sectional observational study. place of study was the icu of square hospitals ltd. and icu of bangabandhu sheikh mujib medical university (bsmmu). all patients who were diagnosed as a case of ventilator associated pneumonia (vap) in icu during the one year study period (01.01.2006 to 31.12.2006) were included in this study. data was collected by patient's clinical register, checklist. total number of patients was 43. main objective of our study was to identify the organisms associa ...200919377438
overgrowth conditions: a diagnostic and therapeutic conundrum.over the past five decades, one of the major problems in the treatment of overgrowth syndromes of the hand has been classification. currently, a number of specific conditions with known natural histories have been delineated and reviewed. treatment is highly individualized. amputation is still recommended for grotesque deformities of the upper limb. early aggressive surgery, when indicated, will improve contour, function, and appearance. specific therapeutic techniques include radical debulking, ...200919380062
pseudomonas aeruginosa las quorum sensing autoinducer suppresses growth and biofilm production in legionella species.bacteria commonly communicate with each other by a cell-to-cell signalling mechanism known as quorum sensing (qs). recent studies have shown that the las qs autoinducer n-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-c(12)-hsl) of pseudomonas aeruginosa performs a variety of functions not only in intraspecies communication, but also in interspecies and interkingdom interactions. in this study, we report the effects of pseudomonas 3-oxo-c(12)-hsl on the growth and suppression of virulence factors ...200919383702
in vitro biological activity screening of lycopodium complanatum l. ssp. chamaecyparissus (a. br.) doll.this article reports the results of selected biological activities, including anticholinesterase, antioxidant, antibacterial, antifungal and antiviral properties, of the petroleum ether, chloroform and methanol extracts as well as the alkaloid fraction of lycopodium complanatum l. ssp. chamaecyparissus (a. br.) doll (lcc, lycopodiaceae) growing in turkey. anticholinesterase effect of the extracts was tested against both acetylcholinesterase (ache) and butyrylcholinesterase (bche) at concentratio ...200919384728
verdoheme formation in proteus mirabilis catalase.heme oxidative degradation has been extensively investigated in peroxidases but not in catalases. the verdoheme formation, a product of heme oxidation which inactivates the enzyme, was studied in proteus mirabilis catalase.200919394409
cardiac tamponade caused by polymicrobial gram-negative organisms.polymicrobial gram-negative pericarditis is a rare entity. we describe the first case of suppurative pericarditis with citrobacter diversus and proteus mirabilis.200919395299
integration of pharmacokinetic and pharmacodynamic indices of orbifloxacin in beagle dogs after a single intravenous and intramuscular administration.the pharmacokinetics (pk) and pharmacodynamics (pd) of orbifloxacin were studied in beagle dogs after intravenous (i.v.) and intramuscular (i.m.) administration at a dose of 2.5 mg/kg body weight. an absolute bioavailability of 100.1% +/- 4.76%, a terminal half-life of 4.23 +/- 0.2 h and 3.95 +/- 0.15 h after i.v. and i.m. administration, a steady-state volume of distribution of 1.61 +/- 0.13 liters/kg, and clearance of 0.31 +/- 0.03 liters/h/kg were observed. orbifloxacin showed rapid, concentr ...200919398644
quinine is bactericidal.to determine the antibacterial properties of quinine dihydrochloride and its mic.200919402279
[effect of the drug-resistant attenuated bacillus proteus on cd80 and cd86 protein expression in peripheral blood dendritic cells of hepatitis b patients].to explore the effect of the drug-resistant attenuated bacillus proteus on the protein expression of cd80 and cd86 in peripheral blood dendritic cells (dcs) of hepatitis b patients.200919403426
tourniquets and exsanguinators: a potential source of infection in the orthopedic operating theater?fomites are increasingly being recognised as a source of hospital-acquired infection. we have therefore assessed tourniquets and exsanguinators for the presence of bacterial pathogens in 1 elective and 2 trauma orthopedic hospitals.200919404812
management of complicated infections in the era of antimicrobial resistance: the role of tigecycline.increasing antimicrobial resistance and infection complications pose challenges to optimal antibiotic therapy. paucity of new antibiotics (and the eventual bacterial resistance they face) highlights the critical need for more appropriate use of broadly effective agents, which may help to thwart the dramatic rise in global resistance. single agents that can be combined effectively with others, if needed, promise the simplest overall utility. approved in 2005 to treat complicated skin and intra-ab ...200919405794
antimicrobial activity of some water plants from the northeastern anatolian region of turkey.the antimicrobial activity of methanol and acetone extracts of butomus umbellatus, polygonum amphibium, and two species of the genus sparganium (s. erectum and s. emersum)against three gram-positive, five gram-negative bacteria and one fungus was assessed by the disk diffusion method. the microorganisms used were staphylococcus aureusatcc-29740, escherichia coli atcc-25922, pseudomonas aeruginosa atcc-15442, salmonella typhi nctc-9394, klebsiella pneumoniae nctc-5046, proteus vulgaris atcc-7829, ...200919145212
high anoxia tolerance in the subterranean salamander proteus anguinus without oxidative stress nor activation of antioxidant defenses during reoxygenation.the present study describes a high anoxia tolerance in an amphibian at high temperature. indeed, the subterranean salamander proteus anguinus survived 12 h under anoxia at 12 degrees c. surprisingly, such experimental conditions did not affect p. anguinus oxidative status while muscles and liver antioxidant enzymes activities decreased under 8 h anoxia and only return to basal level during reoxygenation. to test if such adaptation is common in urodels, equivalent experimentations have been condu ...200919148651
enhanced decolorization and biodegradation of textile azo dye scarlet r by using developed microbial consortium-gr.a developed consortium-gr, consisting of proteus vulgaris ncim-2027 (pv) and micrococcus glutamicus ncim-2168 (mg), completely decolorized an azo dye scarlet r under static anoxic condition with an average decolorization rate of 16,666 microg h(-1); which is much faster than that of the pure cultures (pv, 3571 microg h(-1); mg, 2500 microg h(-1)). consortium-gr gave best decolorization performance with nearly complete mineralization of scarlet r (over 90% toc and cod reduction) within 3h, much s ...200919157864
tigecycline in combination with other antimicrobials: a review of in vitro, animal and case report studies.tigecycline has been investigated in combination with other antibacterials against a wide range of susceptible and multiresistant gram-positive and gram-negative bacteria. combinations have been analysed in vitro, in animal models and in human case reports. in vitro, tigecycline combined with other antimicrobials produces primarily an indifferent response (neither synergy nor antagonism). nevertheless, synergy occurred when tigecycline was combined with rifampicin against 64-100% of enterococcus ...200919162449
changes in bacterial isolates from burn wounds and their antibiograms: a 20-year study (1986-2005).our aim is to elucidate shifts in the bacterial spectrum colonising burn wounds and corresponding antibiotic susceptibilities during a 20-year study period.200919167827
antimicrobial activity of some thiourea derivatives and their nickel and copper complexes.five thiourea derivative ligands and their ni(2+) and cu(2+) complexes have been synthesized. the compounds were screened for their in vitro anti-bacterial activity using gram-positive bacteria (two different standard strains of staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, streptococcus pyogenes, bacillus cereus) and gram-negative bacteria (esherichia coli, pseudomonas aeruginosa, enterobacter cloacae, proteus vulgaris, enterobacter aerogenes) and in vitro anti-yeast ...200919169199
infarction of middle third posterior cortex of kidney: a complication of extended pyelolithotomy, intra-operative electrohydraulic lithotripsy and extraction of calyceal stones under vision using stone basket and flexible cystoscope in a spinal cord injury patient - a case report.abstract:200919175924
a study of the structure of the crystalline bacterial biofilms that can encrust and block silver foley catheters.the aim of this study was to examine the structure of the crystalline bacterial biofilms that encrust and block silver/hydrogel-coated latex catheters. scanning electron microscopy was used to examine the crystalline deposits that were found encrusting catheters obtained from six patients undergoing long-term catheterization in a community setting. large populations of bacilli and cocci were seen on all catheters developing on a basal foundation layer of crystalline material. these observations ...200919189089
[brain abscess (80 cases)].cerebral abscesses are focal suppurations of infectious origin developed within the cerebral parenchyma. this is a rare pathology, with a relatively poor prognosis. therapy continues to be debated and the results are inconsistent. the objectives of this study were to define the factors influencing prognosis.200919095272
spread of multidrug-resistant proteus mirabilis isolates producing an ampc-type beta-lactamase: epidemiology and clinical management.a remarkable increase in proteus mirabilis strains producing acquired ampc-type beta-lactamases (cbls) has been observed at ospedale di circolo e fondazione macchi (varese, italy) over the last few years. the epidemiology and treatment outcome of infections associated with this unprecedented spread are reported. from 2004-2006, 2070 p. mirabilis isolates were investigated. extended-spectrum beta-lactamases (esbls) and cbl resistance determinants were identified by gene amplification and direct s ...200919095415
oxygen-limiting conditions enrich for fimbriate cells of uropathogenic proteus mirabilis and escherichia coli.mr/p fimbriae of uropathogenic proteus mirabilis undergo invertible element-mediated phase variation whereby an individual bacterium switches between expressing fimbriae (phase on) and not expressing fimbriae (phase off). under different conditions, the percentage of fimbriate bacteria within a population varies and could be dictated by either selection (growth advantage of one phase) or signaling (preferentially converting one phase to the other in response to external signals). expression of m ...200919114498
discovery of novel 2',3',4'-trihydroxy-2-phenylacetophenone derivatives as anti-gram-positive antibacterial agents.a number of 2',3',4'-trihydroxy-2-phenylacetophenone derivatives were synthesized and examined for growth inhibition of several kinds of bacteria. 2',3',4'-trihydroxy-2-phenylacetophenone itself exhibited no antibacterial activity, but some of its derivatives showed various antibacterial activities depending on functional groups introduced on the 2-phenyl ring. eighteen out of 24 compounds synthesized in this study appeared to possess antibacterial activities against at least two gram-positive s ...200919129641
the impact of topical phenytoin on recalcitrant neuropathic diabetic foot ulceration.to study the impact of topical phenytoin on the healing of recalcitrant neuropathic diabetic foot ulcers in patients with no clinical evidence of ischaemia or infection, and to evaluate its antibacterial effect.200919131916
ampc beta-lactamases.summary: ampc beta-lactamases are clinically important cephalosporinases encoded on the chromosomes of many of the enterobacteriaceae and a few other organisms, where they mediate resistance to cephalothin, cefazolin, cefoxitin, most penicillins, and beta-lactamase inhibitor-beta-lactam combinations. in many bacteria, ampc enzymes are inducible and can be expressed at high levels by mutation. overexpression confers resistance to broad-spectrum cephalosporins including cefotaxime, ceftazidime, an ...200919136439
behavioral biology: inside the mind of proteus?a new study of the escape behavior of the cockroach has found that its spatial variability is based on some underlying regularity. this constrained variability may maximise the effectiveness of the escape strategy.200919138587
a blaveb-1 variant, blaveb-6, associated with repeated elements in a complex genetic structure.bla(veb-6) was found on the proteus mirabilis chromosome in a context similar to those of bla(veb-1a) and bla(veb-1b), in a truncated gene cassette flanked by 135-bp elements and duplications of the 3'-conserved segment of class 1 integrons. a linked aaca4-aadb-dfra1-orfc cassette array includes components of tn1331, illustrating the complex mosaicism of multiresistance regions.200919139283
vaccination with proteus toxic agglutinin, a hemolysin-independent cytotoxin in vivo, protects against proteus mirabilis urinary tract infection.complicated urinary tract infections (uti) caused by proteus mirabilis are associated with severe pathology in the bladder and kidney. to investigate the roles of two established cytotoxins, the hpma hemolysin, a secreted cytotoxin, and proteus toxic agglutinin (pta), a surface-associated cytotoxin, mutant analysis was used in conjunction with a mouse model of ascending uti. inactivation of pta, but not inactivation of hpma, resulted in significant decreases in the bacterial loads of the mutant ...200919029299
identification of plasmid-mediated ampc beta-lactamases in escherichia coli, klebsiella spp., and proteus species can potentially improve reporting of cephalosporin susceptibility testing results.the goal of this study was to determine if the interpretations of extended-spectrum and advanced-spectrum cephalosporins (escs and ascs, respectively) for isolates of enterobacteriaceae would be impacted by the results of aminophenylboronic acid (apba) testing. fifty-three isolates of escherichia coli, 21 klebsiella species, and 6 proteus species that were resistant to at least one esc were tested by disk diffusion with ceftazidime and cefotetan disks with and without apba. ceftazidime disks wit ...200919036936
microbiological and toxicological effects of perla black bean (phaseolus vulgaris l.) extracts: in vitro and in vivo studies.we investigated the microbiological and toxicological effects of three perla black bean extracts on the growth and culture of selected pathogenic microorganisms, the toxicity over vero cell lines and an in vivo rat model. three different solvents were used to obtain perla black bean extracts. all three perla black bean extracts were tested for antibacterial and antiparasitic activity and further analysed for intrinsic cytotoxicity (ic(50)). methanol perla black bean extract was used for acute to ...200919053992
a fatty acid messenger is responsible for inducing dispersion in microbial biofilms.it is well established that in nature, bacteria are found primarily as residents of surface-associated communities called biofilms. these structures form in a sequential process initiated by attachment of cells to a surface, followed by the formation of matrix-enmeshed microcolonies, and culminating in dispersion of the bacteria from the mature biofilm. in the present study, we have demonstrated that, during growth, pseudomonas aeruginosa produces an organic compound we have identified as cis-2- ...200919074399
growth and aroma contribution of microbacterium foliorum, proteus vulgaris and psychrobacter sp. during ripening in a cheese model medium.the growth and aroma contribution of microbacterium foliorum, proteus vulgaris and psychrobacter sp., some common but rarely mentioned cheese bacteria, were investigated in a cheese model deacidified by debaryomyces hansenii during the ripening process. our results show that these bacteria had distinct growth and cheese flavour production patterns during the ripening process. p. vulgaris had the greatest capacity to produce not only the widest variety but also the highest quantities of volatile ...200919083231
case study: chronic femoropopliteal prosthetic graft infection with exposed graft.one of the most feared complications following vascular reconstruction is infection due to the attendant risks of limb loss, sepsis, or death. the reported incidence of infection following infrainguinal prosthetic graft infection is 2.5% with associated mortality rates and amputation rates of 18% and 41%, respectively. there are several options in treating infected prosthetic infrainguinal bypass grafts. some authors have advocated complete removal of the infected graft with concomitant in situ ...200919190040
polyoxygenated methyl cyclohexanoids from a terrestrial ampelomyces fungus.seven structurally related new polyoxygenated methyl cyclohexanoids, ampelomins a-g (1-7), were isolated from the mycelial solid culture of a soil-derived ampelomyces fungus. their structures were determined by spectroscopic and chemical means. ampelomins a (1), c (3), e (5), and g (7) exhibited weak activity against alpha-glucosidase with ic(50) values of 1.74-5.93 mm, and ampelomin a (1) showed moderate antibacterial activity with mic(90) values ranging from 202.4 to 1015.9 microm. a plausible ...200919193024
significance of the cyclic structure and of arginine residues for the antibacterial activity of arenicin-1 and its interaction with phospholipid and lipopolysaccharide model membranes.arenicin-1 (ar-1) is a beta-sheeted antimicrobial peptide from the marine lugworm arenicola marina. to elucidate the significance of its unique 18-residue cyclic structure and of six cationic arginines for its biological activity and its interaction with biomembranes, we synthesized one linear peptide in which the two cysteines were exchanged for serines (c/s-ar-1) and a cyclic peptide in which all arginines were replaced by lysines (r/k-ar-1). we addressed antibacterial and hemolytic activities ...200919199831
isolation and identification of antibacterial compounds from thymus kotschyanus aerial parts and dianthus caryophyllus flower buds.the aerial parts of thymus kotschyanus boiss. and hohen. (lamiaceae) and flower buds of dianthus caryophyllus l. (caryophyllaceae) have been traditionally implemented in the treatment of wounds, throat and gum infections and gastro-intestinal disorder by the indigenous people of northern iraq, although the compounds responsible for the medicinal properties have not been identified. in this study, antibacterial compounds from both plants were isolated and characterized, and the biological activit ...200919200700
community-acquired urinary tract infections in southern turkey: etiology and antimicrobial resistance.in this study, we compared the distribution and antibiotic susceptibility patterns of bacterial strains isolated from patients with community-acquired urinary tract infections in southern turkey, hatay, during 2004 and 2005. the majority (82.3%) of the isolates were from women while the remaining (17.7%) were from men. of all samples tested, 49% were culture-positive with a bacterial pathogen. the most frequently isolated bacterial species were escherichia coli (45.1%), coagulase-negative staphy ...200919203547
regioselective synthetic approaches towards 1,2,8,9-tetraazadispiro[4.1.4.2]trideca-2,9-dien-6-ones of potential antimicrobial properties.reaction of 2,5-bis(arylmethylidene)cyclopentanones 1a-d with nitrilimines (generated in situ via triethylamine dehydrohalogenation of the corresponding hydrazonoyl chlorides 2a,b) in 1:2 molar ratio proceeds in a high regioselective manner affording monocycloadducts 3 and dicycloadducts in the form of two isomers 4, 5. single crystal x-ray diffraction studies of the isolated crystalline form of 3c support the established structure and indicate that the formed product is 7e, 4s, 5r. antimicrobia ...200919211175
interaction of intracorporeal lithotripters with proteus mirabilis inoculated inside artificial calcium and struvite stones.renal calculi may contain bacteria that can remain active inside the stone and produce bacteremia and/or endotoxemia after lithotripsy. urinary tract infection associated with urinary stones represents high morbidity. the purpose of this research was to use novel artificial struvite stones inoculated with living bacteria and to study the effect of four different intracorporeal lithotripters on bacterial inactivation after in vitro lithotripsy.200919216637
[distribution and drug resistance analysis of bacteria in different wound infections].to analyze the distribution and drug resistance of bacteria in different wound infections and provide evidence for wound infection control in subtropical regions.200919218119
antimicrobial susceptibility pattern of urinary tract pathogens.microbial drug resistance is a major problem in the treatment of infectious diseases worldwide. the purpose of this survey is to determine the prevalence of the type of bacterial agents that cause urinary infection and to assess the antimicrobial sensitivity pattern in the urmia medical university, iran. in the period between 2005 and 2006, urine cultures collected were analyzed. positive culture was defined as growth of a single bacterial species with colony count of > 100,000 cfu/ml. stratific ...200919237813
1h-nuclear magnetic resonance spectroscopy for identifying and quantifying common uropathogens: a metabolic approach to the urinary tract infection.to address the shortcomings of urine culture for the diagnosis of urinary tract infection (uti), we used 1h-nuclear magnetic resonance (nmr) spectroscopy for identifying and quantifying escherichia coli, pseudomonas aeruginosa, klebsiella pneumonia and proteus mirabilis.200919239442
tigecycline.infection with resistant pathogens can adversely affect clinical, microbiological and economic outcomes. new agents for the treatment of many such serious infections are acutely needed in hospital practice. tigecycline is a novel glycylcycline antimicrobial for intravenous use. it has an expanded broad-spectrum antibacterial activity including multi-drug resistant pathogens, like methicillin-resistant staphylococcus aureus , vancomycin-resistant enterococci , multi-drug resistant streptococcus p ...200919242083
proteus syndrome: high-resolution ct and ct pulmonary densitovolumetry findings.cystic transformation of the lungs in proteus syndrome is considered an important manifestation of this disease. we describe a case of an 11-year-old girl with a diagnosis of proteus syndrome with lung involvement. low-dose multidetector computed tomography (ct) revealed extensive diffuse cystic lung disease with left lung predominance, affecting mostly the lower lung zones. the cystic lesions had various sizes and variable wall thickness. postprocessing using ct histogram densitometric volumetr ...200919242304
doripenem activity tested against a global collection of enterobacteriaceae, including isolates resistant to other extended-spectrum agents.the emergence and rapid dissemination of extended-spectrum beta-lactamase (esbl)-producing isolates among enterobacteriaceae coupled with increasing prevalence of stably derepressed and plasmid-borne ampc producers have rendered broad-spectrum cephalosporins and beta-lactam/beta-lactamase inhibitor combinations less effective. this scenario has required the use of carbapenems for treatment of infections caused by such organisms. in this study, the in vitro activities of doripenem and comparator ...200919249175
characteristics and clinical significance of bacterial colonization of ureteral double-j stents in children.to determine the frequency, type of pathogen and clinical significance of bacterial colonization of double-j stents after pyeloplasty in children.200919251483
the dienes phenomenon: competition and territoriality in swarming proteus mirabilis.when two different strains of swarming proteus mirabilis encounter one another on an agar plate, swarming ceases and a visible line of demarcation forms. this boundary region is known as the dienes line and is associated with the formation of rounded cells. while the dienes line appears to be the product of distinction between self and nonself, many aspects of its formation and function are unclear. in this work, we studied dienes line formation using clinical isolates labeled with fluorescent p ...200919251852
pneumoureter. 200919254678
new plasmid-mediated quinolone resistance gene, qnrc, found in a clinical isolate of proteus mirabilis.since the discovery of qnra in 1998, two additional qnr genes, qnrb and qnrs, have been described. these three plasmid-mediated genes contribute to quinolone resistance in gram-negative pathogens worldwide. a clinical strain of proteus mirabilis was isolated from an outpatient with a urinary tract infection and was susceptible to most antimicrobials but resistant to ampicillin, sulfamethoxazole, and trimethoprim. plasmid phs10, harbored by this strain, was transferred to azide-resistant escheric ...200919258263
detection of bacterioplankton in immersed cadavers using selective agar plates.we measured bacterioplankton in blood from cadavers retrieved from the sea (n=12), near estuaries (n=4), rivers (fresh water, n=8) and from bathtubs (n=4) as well as from non-drowned victims (n=10) discovered near aquatic environments. blood from 11 victims drowned in seawater developed bioluminescent and/or blue colonies (oxidase test positive) on selective media containing 2-4% nacl. homology analyses of the 16s rrna gene showed that all of them were marine bacteria (genera: photobacterium, vi ...200919261520
[evaluation of the microscan negcombo panel type 44 for detection of extended-spectrum beta-lactamase among clinical isolates of escherichia coli, klebsiella species, and proteus mirabilis].accurate and rapid detection of extended-spectrum beta-lactamases (esbls) is important in guiding proper antimicrobial therapy for infected patients. we evaluated the performance of microscan negcombo type 44 panel (dade behring, usa), which was developed to confirm esbl-producing enterobacteriaceae using ceftazidime/clavulanate and cefotaxime/clavulanate.200919262076
[a boy with abdominal pain and when urinating. urinary stones in a mega urethra]. 200919271445
growth inhibition of pathogenic bacteria and some yeasts by selected essential oils and survival of l. monocytogenes and c. albicans in apple-carrot juice.food safety is a fundamental concern of both consumers and the food industry. the increasing incidence of foodborne diseases increases the demand of using antimicrobials in foods. spices and plants are rich in essential oils and show inhibition activity against microorganisms, which are composed of many compounds. in this research, effects of garlic, bay, black pepper, origanum, orange, thyme, tea tree, mint, clove, and cumin essential oils on listeria monocytogenes aufe 39237, escherichia coli ...200919278342
[change of mating type induced by actinomycin d in the ciliate dileptus anser].effect of actinomycin d on the expression and inheritance of mating types (mts) in mature laboratory clones of the ciliate dileptus anser was studied. each mature clone of these ciliates isolated from natural population appears to belong to one of the three complementary mts - mt i, mt ii or mt iii. its mt does not vary in the course of further laboratory cultivation of the clone in a series of vegetative generations. however, after treatment with actinomycin d (15 microg/ml, 3 days), such clone ...200919281052
catheter-related urinary tract infection in patients suffering from spinal cord injuries.urinary tract infection is commoner in patients with spinal cord injuries because of incomplete bladder emptying and the use of catheters that can result in the introduction of bacteria into the bladder. 145 patients suffering from spinal cord injuries, admitted to the institute for physical medicine and rehabilitation, centre for paraplegia of the clinical centre of the university of sarajevo, were included. the patients were divided in three groups according to the method of bladder drainage: ...200919284388
surveillance of intrahospital infections at the clinic for gynaecology and obstetrics.intrahospital infections (ihi) and antibiotics resistance are the problems which exist in virtually all hospitals in the world. the main aim of the present research is establishing of epidemiological surveillance over occurrence of ihi at the clinic for gynaecology and obstetrics at the university clinical center tuzla and thus identifies: types of bacteria which cause ihi, types of infection according to anatomical localization and research resistance organisms causing of ihi on antimicrobial d ...200919284398
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