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in vitro viability of external eye microbial flora in hydroxy-propyl-methylcellulose.the aim of this study was to verify the in vitro influence of various dilutions of a viscoelastic substance containing 2% hydroxy-propyl-methylcellulose (hpmc) on the viability of some microbial strains representative of the normal flora of the external eye. pure reference strain cultures of candida albicans, pseudomonas aeruginosa, propionibacterium acnes, staphylococcus aureus, staphylococcus epidermidis and a fresh clinical isolate of proteus mirabilis were selected for this study. serial two ...199910420112
[the clinico-morphological characteristics of salmonellosis typhimurium in children].clinical and morphological features were studied of the course of gastrointestinal and generalized forms of salmonellosis. the grave gastrointestinal form runs its course against the unfavourable premorbid background getting associated with other flora: proteus, citrobacter, klebsiella, staphylococcus aureus. a distinguishing feature of the generalized form is its variegated clinical symptomatology. noted in some instances is an acute onset of the illness with a clinical picture of purulent meni ...199910424015
does bile of patients with acute gallstone pancreatitis cause pancreatic inflammatory lesions? a study of the pancreatic toxicity of choledochal secretions collected at ercp.bile flow into the pancreatic duct has been proposed as the cause of acute biliary pancreatitis. however, the pancreatic toxicity of choledochal bile from patients with acute gallstone pancreatitis has not been studied.199910425414
antimicrobial activity of tea as affected by the degree of fermentation and manufacturing season.bacillus subtilis, escherichia coli, proteus vulgaris, pseudomonas fluorescens, salmonella sp. and staphylococcus aureus were used to test the antimicrobial activity of tea flush extract and extracts of various tea products. among the six test organisms, p. fluorescens was the most sensitive to the extracts, while b. subtilis was the least sensitive. in general, antimicrobial activity decreased when the extents of tea fermentation increased. the antimicrobial activities of tea flush extract and ...199910426448
demographic, clinical, and treatment parameters influencing the outcome of acute cystitis.a meta-analysis of six double-blinded clinical trials was undertaken to identify risk factors associated with bacteriologic outcome in 3,108 women with acute cystitis. eleven antibiotic regimens were used, including ciprofloxacin, ofloxacin, norfloxacin, trimethoprim-sulfamethoxazole, and nitrofurantoin. entry criteria for all studies were identical. among 2,409 patients who were defined to be valid for efficacy analysis, pathogens included escherichia coli (78.6%), staphylococcus saprophyticus ...199910433573
human gamma delta t cells recognize alkylamines derived from microbes, edible plants, and tea: implications for innate immunity.approximately 4% of peripheral blood t cells in humans express a t cell receptor with markedly restricted germline gene segment usage (v gamma 2 v delta 2). remarkably, these t cells expand 2- to 10-fold (8%-60% of all circulating t cells) during many microbial infections. we show here that these t cells recognize a family of naturally occurring primary alkylamines in a tcr-dependent manner. these antigenic alkylamines are secreted to millimolar concentrations in bacterial supernatants and are f ...199910435579
an epr investigation of surfactant action on bacterial membranes.the effects of the surfactants, alcohol ethoxylate, amine ethoxylate, amine oxide and sds on cell membranes were investigated using the lipid soluble spin label 5-doxyl stearic acid (5-ds). electron paramagnetic resonance (epr) spectroscopy revealed that the action of the surfactants was to significantly increase membrane fluidity of proteus mirabilis, staphylococcus aureus and saccharomyces cerevisiae. the action of these surfactants as biocides was investigated and found to be dependent on the ...199910436922
microbes and sequestered substances as mechanisms for disease: bartonella and l-forms as common global etiological agents.the pathogenicity of microbes may be determined by substances sequestered from blood and bound to their constituent lipid. the brain may not perceive substances sequestered by microbes, to interfere with control to maintain normal levels. pathological conditions can be induced as organisms exposed to antimicrobial substances/conditions and/or deprived of nutrients essential to cell wall synthesis, disintegrate to free lipid-bound compounds and produce l-forms that can deplete nutrients as they r ...199910465665
in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group.we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ...199910467641
investigation of influence of proteus mirabilis lps polysaccharide part on the murine immune cells activation.the biological activities were investigated of proteus mirabilis lipopolysaccharides (lpss) and their fragments, namely, the induction of nitric oxide (no) and interleukin 1 (il-1) synthesis by murine macrophages and the proliferation of murine spleen cells. the o-specific polysaccharide (f1), core oligosaccharide (f2) and lipid a were as effective as intact lps in stimulating murine macrophages to produce no. il-1 synthesis was also induced by all studied types of endotoxins (s, ra, re) and par ...199910470447
paediatric nosocomial urinary tract infection at a regional hospital.from a total of 26,603 admissions to the paediatric wards, 1360 paediatric nosocomial urinary tract infections (pnuti) were identified during a 5-year retrospective chart review at the sfgh. the ages ranged from 3 days to 13 years, with 46% boys and 54% girls. the highest rates of pnuti per service per 100 admissions were seen in the nursery (11.28) followed by paediatric surgery (2.89) and paediatric medicine (2.86). although the greatest number of pnuti occurred in the nursery, comparison betw ...199910481961
proteus mirabilis osteomyelitis of the ribs in a girl with sickle cell anaemia. 199910485315
canine distemper virus infection in binturongs (arctictis binturong).two binturongs (arctictis binturong) kept in outdoor exhibits at everland zoological gardens in korea died within 10 days of the onset of clinical signs that included depression, dyspnoea, diarrhoea and convulsions. on necropsy, the significant gross findings were limited to the lungs and the gastrointestinal tract. proteus vulgaris was isolated from the lung of one animal. histopathologically, diffuse severe bronchointerstitial pneumonia with secondary bacterial infection was noted in the lungs ...199910486167
the facilitating effect of cigarette smoke on the colonization of instilled bacteria into the tracheal lumen in rats and the improving influence of supplementary vitamin e on this process.the aim of the present study was to investigate the direct influence of cigarette smoke on the bacterial colonization of the lower respiratory tract and the effect of supplementary vitamin e on the colonization of instilled bacteria into the trachea of rats that do not have the chronic airway pathology associated with smoking.199910489666
[development of a vaccine for treatment of urinary tract infections].receiving uroinfections are predominantly treated with antibiotics and chemotherapeutics. an effective alternative treatment can be immunotherapy with inactivated bacteria. the authors have focused on the development of polybacterial vaccine with both specific and nonspecific immunostimulatory activity. the vaccine contains killed bacteria which most frequently occur as an etiological agent in urinary tract infections. determining for the selection of particular strains for the vaccine preparati ...199910493005
[clinical success of treatment of chronic otitis media using topical and peroral administration of ofloxacin].the paper presents results of the treatment of 32 patients (35 ears) with chronic otitis media and long lasting purulent otorrhea with local and oral ofloxacin. the patients were divided in two groups. group a included 17 patients (49%), with previous middle ear surgery. group b comprised 15 patients (18 ears, 51%) treated with local and systemic antimicrobial drugs. ofloxacin was administered after taking the swab sample, and the duration of treatment was the same in all patients. the most freq ...199910494153
genomic subtractive hybridization and selective capture of transcribed sequences identify a novel salmonella typhimurium fimbrial operon and putative transcriptional regulator that are absent from the salmonella typhi genome.salmonella typhi, the etiologic agent of typhoid fever, is adapted to the human host and unable to infect nonprimate species. the genetic basis for host specificity in s. typhi is unknown. the avirulence of s. typhi in animal hosts may result from a lack of genes present in the broad-host-range pathogen salmonella typhimurium. genomic subtractive hybridization was successfully employed to isolate s. typhimurium genomic sequences which are absent from the s. typhi genome. these genomic subtracted ...199910496884
quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from pseudomonas aeruginosa and other gram-negative bacteria.in cell-free pseudomonas aeruginosa culture supernatants, we identified two compounds capable of activating an n-acylhomoserine lactone (ahl) biosensor. mass spectrometry and nmr spectroscopy revealed that these compounds were not ahls but the diketopiperazines (dkps), cyclo(deltaala-l-val) and cyclo(l-pro-l-tyr) respectively. these compounds were also found in cell-free supernatants from proteus mirabilis, citrobacter freundii and enterobacter agglomerans [cyclo(deltaala-l-val) only]. although ...199910510239
antibiotic sensitivity of bacteria associated with community-acquired urinary tract infection in britain.twelve laboratories in different parts of britain each supplied approximately 80 consecutive urinary bacterial isolates from community patients. all strains were identified by a central laboratory, where sensitivity to a variety of orally administered antimicrobials was determined by microtitre broth dilution. 65.1% of isolates were escherichia coli, 23.4% 'coliforms' other than e. coli, 4.6% proteus and morganella spp., 1.8% pseudomonas spp., 2.4% enterococci, 0.7% group b streptococci, 1.5% co ...199910511403
association of rickettsia helvetica with chronic perimyocarditis in sudden cardiac death.rickettsia helvetica is the only non-imported rickettsia found in scandinavia. it was first detected in ixodes ricinus ticks, but has never been linked to human disease. we studied two young swedish men who died of sudden cardiac failure during exercise, and who showed signs of perimyocarditis similar to those described in rickettsial disease.199910513711
in vitro antibacterial activity of fk041, a new orally active cephalosporin.the in vitro activity of fk041, a new orally active cephem antibiotic, against a wide variety of clinical isolates of bacteria was investigated and compared with those of cefdinir (cfdn) and cefditoren (cdtr). fk041 exhibited broad spectrum activity against reference strains of gram-positive and gram-negative aerobes and anaerobes. fk041 was active against clinical isolates of gram-positive organisms except enterococcus faecalis with mic90s less than 1.56 microg/ml. fk041 was more active than cf ...199910513845
structure of a new acidic o-antigen of proteus vulgaris o22 containing o-acetylated 3-acetamido-3,6-dideoxy-d-glucose.the acidic o-specific polysaccharide of proteus vulgaris o22 was studied using 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments, and the following structure for the branched pentasaccharide repeating unit was established: [sequence: see text] where qui3nac is 3-acetamido-3,6-dideoxyglucose, o-acetylation of quinac at position 4 is stoichiometric and at position 2 nonstoichiometric. serological relatio ...199910515053
structure and cross-reactivity of the o-antigen of proteus vulgaris o8.a high-molecular-mass o-specific polysaccharide was obtained by mild acid degradation of proteus vulgaris o8 lipopolysaccharide followed by gel permeation chromatography. studies of the polysaccharide by sugar and methylation analyses and 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments, demonstrated the presence of a tetrasaccharide repeating unit having the following structure: [sequence: see text] ...199910515057
abolition of swarming of proteus. 199910515741
the use of chromagar orientation as a primary isolation medium with presumptive identification for the routine screening of urine specimens.the aim of the study was to compare the use of a novel differential culture medium chromagar, for both primary isolation and presumptive identificaton, with the method currently used in our laboratory for screening mid-stream-urine samples (msu). routine methods (rm) included blotting paper imprinting of all specimens and additional quantitative culture on cysteine lactose electrolyte-deficient agar (cled) for selected samples together with microbact 12e for further identification. the chromagar ...199910519322
the structure of the core part of proteus mirabilis o27 lipopolysaccharide with a new type of glycosidic linkage.the structural assignment of the intact lipopolysaccharide core from proteus mirabilis o27 has been completed based on a combination of chemical degradation studies, nmr spectroscopy and es-ms spectroscopy. the overall core structure is as follows: [formula: see text] where all sugars are in the pyranose form except the n-acetylglycosamine residue, hep refers to l-glycero-alpha-d-manno-heptopyranose and alpha-ddhep to d-glycero-alpha-d-manno-heptopyranose. bold italics indicate non-stoichiometri ...199910520257
structure of an acidic o-specific polysaccharide of proteus mirabilis o5.the following structure of the o-specific polysaccharide of proteus mirabilis o5 was established by 1h and 13c nmr spectroscopy at 500 mhz, including two-dimensional cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments: [formula: see text] where o-acetylation of alpha-d-glcnac at both positions is nonstoichiometric.199910520266
molecular mechanisms of interaction of rabbit cap18 with outer membranes of gram-negative bacteria.the mechanism of interaction of the cationic antimicrobial protein (18 kda), cap18, with the outer membrane of gram-negative bacteria was investigated applying transmission electron microscopy and voltage-clamp techniques on artificial planar bilayer membranes. electron micrographs of bacterial cells exposed to cap18 showed damage to the outer membrane of the sensitive escherichia coli strains f515 and atcc 11775, whereas the membrane of the resistant proteus mirabilis strain r45 remained intact ...199910521271
evolutionary changes reflected by the cellular amino acid composition.comparison of the amino acid composition of cell-proteins using 17 amino acids has been used to investigate the biological evolution of organisms such as bacteria, blue-green alga, green alga, fungi, slime mold, protozoa and vertebrates. the degree of difference in the amino acid ratios between any two groups reflects the degree of divergency in biological evolution. the amino acid composition of the gram-negative bacteria (escherichia coli, klebsiella, proteus, and vibrio alginolyticus) was ide ...199910524278
proteus mirabilis viability after lithotripsy of struvite calculi.we tested the hypotheses that proteus mirabilis viability of struvite calculi differs after exposure to different lithotripsy modalities and that the photothermal mechanism of holmium:yag lithotripsy is antibacterial.199910524893
characteristics of pathogens causing urinary tract infections in hospitals in north america: results from the sentry antimicrobial surveillance program, 1997.urinary tract infection (uti) is common and involves pathogens with changing susceptibility patterns. the sentry antimicrobial surveillance program evaluates international pathogen incidence patterns to detect and manage the emergence of resistant strains. we describe the antimicrobial resistance patterns among 1617 pathogens recovered from utis during the third-quarter of 1997 in north america (united states and canada), as part of this worldwide program. the isolates were tested against more t ...199910529882
identification and molecular analysis of the gene encoding rickettsia typhi hemolysin.rickettsia typhi, the causative agent of murine typhus, grows directly within the host cell cytoplasm, accumulating a large number of progeny, and eventually lyses the cells. typhus group rickettsiae (r. typhi and r. prowazekii) adhere to and lyse human and sheep erythrocytes. however, the molecular mechanism underlying erythrocyte lysis by r. typhi has not been defined. here we describe the cloning and nucleotide sequence analysis of the gene (tlyc) encoding a hemolysin from r. typhi. dna seque ...199910531273
a child with hemimegalencephaly, hemihypertrophy, macrocephaly, cutaneous vascular malformation, psychomotor retardation and intestinal lymphangiectasia--a diagnostic dilemma.although the clinical delineation of the majority of overgrowth syndromes is straightforward, we believe there is a subset of patients with overlapping features from a number of overgrowth syndromes. we report a patient with hemimegalencephaly, hemihypertrophy, macrocephaly, vascular lesions, psychomotor retardation and intestinal lymphangiectasia. the clinical history and findings posed a diagnostic dilemma as the features overlapped between several conditions, namely macrocephaly-cutis marmora ...199910532178
an exploration of the accuracy of an invasive method for measuring the volume of specimens of amoeba proteus.a method is described for measuring the volume of individual specimens of amoeba proteus which utilizes an easily constructed compressor to flatten the specimen to a known thickness. the microscopic image of the flattened specimen is captured on tape, digitized and analysed with the nih image software. the results from one specimen are given to illustrate the sources and magnitude of errors affecting these volume measurements.199910540260
diversity of tem mutants in proteus mirabilis.in a survey of resistance to amoxicillin among clinical isolates of proteus mirabilis, 10 tem-type beta-lactamases were characterized: (i) the well-known penicillinases tem-1 and tem-2, the extended-spectrum beta-lactamases (esbls) tem-3 and tem-24, and the inhibitor-resistant tem (irt) tem-44 and (ii) five novel enzymes, a penicillinase tem-57 similar to tem-1, an esbl tem-66 similar to tem-3, and three irts, tem-65, tem-73, and tem-74. the penicillinase tem-57 and the esbl tem-66 differed from ...199910543745
antimicrobial activities of several parts of pinus brutia, juniperus oxycedrus, abies cilicia, cedrus libani and pinus nigra.in this study, the antimicrobial activities of several parts of various trees grown in the kahramanmaraƟ region of turkey were investigated by the disc diffusion method. chloroform, acetone and methanol extracts of leaves, resins, barks, cones and fruits of pinus brutia ten., juniperus oxycedrus l., abies cilicia ant. & kotschy carr., cedrus libani a. rich. and pinus nigra arn. were prepared and tested against bacillus megaterium dsm 32, bacillus subtilis img 22, bacillus cereus fmc 19, escheric ...199910548751
minimal debridement in the treatment of fournier's gangrene.scrotal and perineal gangrene of the male genitalia was first described by fournier in 1883. treatment of the disease remains surgical despite the availability of modern antibiotics. many authors advocate a wide debridement of all tissue superficial to the involved fascial layers. we have reviewed our experience of using wide and minimal debridement as the surgical technique of choice. in our retrospective study, nine patients were diagnosed and treated over a 2-year period for fournier's gangre ...199910551751
the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae.the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ...199910555323
clostridium difficile toxins may augment bacterial penetration of intestinal epithelium.clostridium difficile can be recovered from many high-risk hospitalized patients receiving broad-spectrum antibiotic therapy. clostridium difficile toxins a and b have been associated with increased intestinal permeability in vitro and there is growing evidence that increased intestinal permeability may be a common mechanism whereby enteric bacteria penetrate the intestinal epithelium.199910555639
preseptal cellulitis secondary to proteus species: a case report and review.preseptal cellulitis is a serious ocular condition that--if left untreated--has the potential to cross the septal barrier, spread to the posterior orbit, and may result in fatal complications. because it is difficult to determine the pathogen responsible for any cellulitis without aspirating a culture sample, treatment is usually instituted by an assumption of the most common causative organisms, staphylococcus or streptococcus.199910561925
effect of thermal injury with pseudomonas aeruginosa infection on pulmonary and systemic bacterial clearance.despite improvements in burn wound care, infections, particularly pneumonia, remain a major hurdle to recovery from thermal injury. after burns, a variety of systemic immune and inflammatory changes contribute to the risk of infection. clinically, infection coupled with burn injury seems to adversely affect susceptibility to subsequent infection.199910568721
serum immunoglobulin response and protection from homologous challenge by proteus mirabilis in a mouse model of ascending urinary tract infection.we tested the hypothesis that experimental proteus mirabilis urinary tract infection in mice would protect against homologous bladder rechallenge. despite production of serum immunoglobulin g (igg) and igm (median titers of 1:320 and 1:80, respectively), vaccinated (infected and antibiotic-cured) mice did not show a decrease in mortality upon rechallenge; the survivors experienced only modest protection from infection (mean log(10) number of cfu of p. mirabilis nal(r) hi4320 per milliliter or gr ...199910569791
the structure of the core part of proteus vulgaris ox2 lipopolysaccharide.the identity of a novel structural component, an open-chain acetalic linkage, in the core part of the lipopolysaccharide (lps) from proteus vulgaris serotype ox2 has been determined by extensive nmr spectroscopic analysis of fragments isolated after mild acid hydrolysis of the intact lps. the open-chain n-acetylgalactosamine fragment is substituted in the 4-position by non-stoichiometric amounts of a beta-galactopyranose residue and the overall structure of the core is as follows: [formula: see ...199910573861
[advances and prospects of a combined therapy of acute radiation injury in experiments].we present the results of a long-standing experimental development of ways and means for acute radiation sickness treatment that have been authorized for application in medicine and are mostly aimed at large-scale accidental injuries. the paper describes means for early treatment (prodigiosan, desoxynate, typhoid vaccine, proteus vaccine), a myeolopoiesis stimulant (estradiol dipropionate), a detoxication procedure (hemosorption), substitution therapy with bone marrow cells and peripheral blood ...199910576028
cartilage degradation by hyaluronate lyase and chondroitin abc lyase: a maldi-tof mass spectrometric study.matrix-assisted laser desorption ionization and time-of-flight mass spectrometry (maldi-tof ms) has been used to investigate degradation products of two selected polysaccharides of cartilage (chondroitin sulfate and hyaluronic acid). testicular hyaluronate lyase and chondroitin abc lyase were used for enzymic digestion of both polysaccharides as well as of cartilage specimens. polysaccharide solutions and cartilage supernatants were assayed by positive and negative maldi-tof ms. especially chond ...199910576924
evaluation of the in vitro antimicrobial activity of cefepime compared to other broad-spectrum beta-lactams tested against recent clinical isolates from 10 chinese hospitals. chinese antimicrobial resistance study group.a surveillance study was initiated in china in 1998 in which 10 medical centers participated. the susceptibility profiles of 996 commonly occurring pathogens belonging to 10 different species groups were tested by the etest (ab biodisk, solna, sweden) against six broad-spectrum beta-lactam antimicrobial agents (cefepime, ceftazidime, ceftriaxone, imipenem, cefoperazone/sulbactam and piperacillin or oxacillin). quality control was closely monitored and cefepime- and/or imipenem-resistant enteroba ...199910579094
molecular recognition of synthetic siderophore analogues: a study with receptor-deficient and fhu(a-b) deletion mutants of escherichia coli.the biological activity of six synthetic siderophore analogues (two dihydroxamates, two trihydroxamates, one tetrahydroxamate and one 3-hydroxy-4(1h)pyridinone) has been studied in escherichia coli, morganella morganii 13 and proteus mirabilis 8993 strains by using growth promotion tests. various transport-deficient mutants of e. coli were used to study the route of entry into gram-negative bacteria. the results indicated that the synthetic hydroxamate compounds are transported via fhu-mediated ...199910581683
nephrobronchial fistula secondary to xantogranulomatous pyelonephritis.we report a case of staghorn nephrolithiasis that evolved into xanthogranulomatous pyelonephritis with perinephric abscess, nephrobronchial fistula, and lung abscess. the patient was an intravenous drug abuser who tested positive for human immunodeficiency virus, without evidence of acquired immunodeficiency syndrome. he presented with a 2-month history of untreated repeated episodes of left flank pain and hyperpyrexia. treatment involved left nephrectomy, debridement of abscess, tube drainage, ...199910585069
gram-negative bacillary meningitis in adult post-neurosurgical patients.to assess the clinical features and therapeutic outcomes of gram-negative bacillary meningitis (gnbm) in adult postneurosurgical patients.199910595761
intestinal protozoa and associated bacteria in captive houbara bustards (chlamydotis undulata) in the united arab emirates.a protozoal survey was carried out in 114 captive houbara bustards (chlamydotis undulata) in the united arab emirates. trichomonas gallinarum, chilomastix gallinarum, giardia spp. and lophomonas spp. were detected from faecal samples of 49 (43%) captive houbara bustards. culture and identification studies were carried out to assess associated bacteria. the bacteria isolated from birds negative to protozoa included enterococcus spp., aerococcus spp., micrococci spp., proteus spp., enterobacter sp ...199916147554
photoproduction of hydrogen from raw starch using a halophilic bacterial community.the bacterial community designated bc1, which originates from night soil treatment sludge, exhibited a strong ability to produce h2 from raw starch in the light in the presence of 3% nac1. three halophilic or halotolerant bacterial species, vibrio fluvialis, rhodobium marinum, and proteus vulgaris, were isolated from bc1 and identified. the level of h2 production from starch by coculture of v. fluvialis and r. marinum was nearly equal to that by bc1, indicating that these two strains play roles ...199916232577
synthesis, characterization and antimicrobial activity of d metal complexes of some 2-substituted-1h-benzimidazoles.the metal complexes of nine 2-substituted-1h-benzimidazoles (i-ix) with ni(ii), pd(ii), cu(ii), ag(i), zn(ii) salts were synthesized. the compounds were characterized by melting point, analytical data, ir spectroscopy and magnetic susceptibility. the antimicrobial activity of the compounds was determined by the disk diffusion method in mueller-hinton agar on staphylococcus aureusatcc 6538, staphylococcus epidermidis atcc 12228, escherichia coli atcc 8739, klebsiella pneumoniae atcc 4352, pseudom ...199918472907
developmental intestinal aerobic microflora in the kori bustard (ardeotis kori).a study was carried out to investigate the normal aerobic bacterial flora of developing kori bustard (ardeotis kori) chicks, captive bred at the national avian research center, abu dhabi, united arab emirates. faecal samples were collected from 14 birds at different ages from the first day of hatching until 99 days old and were cultured for aerobic bacteria. several bacterial species were isolated from the cultures, they included escherichia coli, streptococcus viridians, enterococcus faecalis, ...199818484014
structure-activity relationships for some diamine, triamine and schiff base derivatives and their copper(ii) complexes.ethylenediamine (en), putrescine (pu), diethylenetriamine (dien), dipropylenetriamine (dpta), spermidine (spmd) and their cu(ii) compounds as well as the schiff bases with 2-furaldehyde (dienoo), 2- thiophenecarboxaldehyde (dienss) and pyrrole-2-carboxaldehyde (diennn) of dien and that of dpta with 2- thiophenecarboxaldehyde (dptass), were prepared and characterised. they were tested against bacillus substilis, bacillus cereus, staphylococcus aureus, escherichia coli, proteus vulgaris and xantho ...199818475868
phytochemical and antimicrobial studies of salvia splendens sello.the phytochemical investigation of antimicrobial agents from locally occurring salvia splendens sello, was carried out. three common solvents i.e. petroleum ether (40-60%), chloroform and methanol were successively used for the extraction of antimicrobial principles from its various parts. three major compounds (namely c1, c2, and c3) were isolated and purified from the active methanol extract of roots by column and thin layer chromatography. gram-positive bacteria (bacillus pumilus, b. subtilis ...199816414814
resistance pattern of clinical isolates from cases of chronic ear infection ii.during present study hundred clinical isolates from cases of ear infection were tested from local population for resistance pattern, using eleven different antibiotics. a considerable percentage of isolates causing ear infection was resistant to number of antimicrobial drugs. the percentages of organisms isolated from ear infection are pseudomonas aeruginosa (50%), proteus species (16%), staphylococcus aureus (15%), klebsiella species (13%) and escherichia coli (6%).199816414816
a zn(ii)-translocating p-type atpase from proteus mirabilis.a mutant of proteus mirabilis had been previously isolated as defective in swarming. the mutation had been found to be in a gene related to the escherichia coli znta gene, which encodes the znta zn(ii)-translocating p-type atpase. in this study the p. mirabilis gene was expressed in an e. coli strain in which the znta gene had been disrupted. the p. mirabilis gene complemented the sensitivity to salts of zinc and cadmium. everted membrane vesicles from the znta-disrupted strain lost atp-driven 6 ...199810353712
[bacterial infections in cancer patients: what should we treat them with when the result of antibiogram is not yet known?].cancer patients are prone to bacterial infections, which frequently on account of severity require prompt administration of antibiotics--even before the result of antibiogram is known. 500 antibiograms obtained from cultures taken from 307 cancer patients treated in general hospital no. 2, bielsko-biala during 1991-1995 were analyzed. marked diversity in isolated bacterial strains was found, the dominant pathogens being gram positive species (mainly staphylococci and streptococci, which constitu ...199810222843
[use of a composite organic biosilicone sorption preparation imosdinit in the treatment of anaerobic infections in surgery].new application sorbent on the base of hydrogel and xerogel of methylsilicone acid with immobilized nitazole in connection with dimexide- imosdynit was investigated in experiment. high efficacy of imosdynit in the treatment of clostridial and non-clostridial anaerobic infection, caused by klebsiella, was established. proteus and blue pus bacillus are nonsensitive to imosdynit. preparation was applied in 56 patients with purulent peritonitis. the frequency of wounds suppuration reduced by 2.4 tim ...199810077942
the management of brain abscess in a developing country: are the results any different?a retrospective study of 57 surgically-managed brain abscesses at the neurosurgical unit, bir hospital during a 6-year period from october 1990 is presented. detailed hospital case notes could be traced in only 37 cases, consisting of 25 males and 12 females with age range from 5 months to 60 years. cryptogenic abscess was the commonest category followed by abscess caused by chronic ear infection. the diagnosis was made with enhanced computed tomography (ct) in all the cases. positive bacteriolo ...199810070424
the antibacterial activity of acetic acid and burow's solution as topical otological preparations.at present there are no topical otological preparations on the world health organisation's essential drug list, largely due to the ototoxic potential of preparations containing aminoglycoside antibiotics. acetic acid and burow's solution have long been used in the treatment of the discharging ear. the aim of this study was to ascertain the antibacterial activity of these two preparations against the most commonly occurring bacteria isolated from discharging ears in our department. twenty fresh i ...199810211213
antibacterial effect of some 2,6-disubstituted 4-anilinoquinazolines.two synthetic 2,6-disubstituted 4-anilinoquinazolines exerted a significant effect on the g+ bacteria bacillus subtilis and staphylococcus aureus. none of 12 tested derivatives influenced escherichia coli, proteus mirabilis and pseudomonas aeruginosa. derivatives having the aromatic ring non-substituted or substituted by bromine, the pyrimidine ring by phenyl, morpholine or piperidine and the aniline skeleton non-substituted or substituted by methyl or amino group exerted a considerable antibact ...199810189231
significance of polymicrobic bacteriuria in a teaching hospital in trinidad.to compare microbial prevalence (mixed versus pure), define microbial combination in mixed urine cultures among inpatients and outpatients and to identify factors that might allow a more objective interpretation of polymicrobic urine cultures.199810189749
infectious dermatitis in a ball python (python regius) colony.seven wild-caught ball pythons (python regius), including six gravid females and one male, were obtained from africa and were housed in a government animal facility in research triangle park, north carolina. upon arrival, the snakes were found to be infested with ticks (aponomma latus), which were manually removed. four weeks following arrival, vesicular skin lesions began to appear on the snakes. despite treatment of all affected female snakes with amikacin (5 mg/kg i.m., every 3 days) and cefo ...199810065857
proteus syndrome with widespread portwine stain naevus.proteus syndrome is a rare condition comprising asymmetrical overgrowth of different parts of the body in association with various cutaneous abnormalities. we describe a 3-year-old boy with proteus syndrome, who presented with hemihypertrophy of the right leg, asymmetric macrodactyly, subcutaneous masses and a widespread portwine stain interspersed with angiokeratomas on the right leg, scrotum and on the middle and left side of the back. doppler ultrasound of the right leg did not show hypercirc ...19989990373
skin bacteriology and the role of staphylococcus aureus in infection.many of the staphylococci and coryneforms that inhabit normal human skin do not cause skin disease. amongst the remainder the mechanisms of pathogenicity vary widely. for proteus, pseudomonas and brevibacterium species proteolysis is a major determinant. the precise role of corynebacterium minutissimum in erythrasma and the propionibacteria in acne is not known. staphylococcus aureus, however, produces a wide range of non-specific agents, such as haemolysins and leucocidins as well as highly spe ...19989990407
genomic fingerprinting of proteus species using repetitive sequence based pcr (rep-pcr).three proteus species p. vulgaris, p. mirabilis and p. penneri have been characterized by repetitive sequence-based pcr. four families of repetitive sequence based primers rep, eric, boxa1r and boxa2r, give specific patterns for each proteus species. species differentiation was best afforded using boxa2r for detection of p. mirabilis, either rep-dt or boxa1r primers for detection of p. penneri and eric primer pair for p. vulgaris.19989990714
comparative in-vitro activities of trovafloxacin, ciproflaxacin, ofloxacin, and broad-spectrum beta-lactams against aerobe blood culture isolates.the in vitro activity of trovafloxacin, a new fluoroquinolone, was compared with that of ciprofloxacin, ofloxacin, fleroxacin, ceftazidime, piperacillin/tazobactam, and meropenem against 613 consecutively recovered blood isolates from recently hospitalized patients. susceptibility testing was performed by agar dilution according to nccls guidelines. test strains included acinetobacter species (n = 26), escherichia coli (n = 137), enterobacter species (n = 27), klebsiella species (n = 42), proteu ...19989987189
[cerebral abscess of otogenic origin located simultaneously in the temporal and cerebellar regions].a 28-year-old male was seen for chronic left ear disease. a brain abscess appeared in the left temporal lobe and later spread to the cerebellum. diagnosis was clinical and radiological. intravenous antibiotic treatment resulted in resolution of the temporal abscess on ct. however, a new abscess that appeared in the left cerebellar hemisphere required neurosurgical debridement. the patient's microbiological cultures and history suggested an otological origin. brain abscess is a serious complicati ...19989951088
bacteriological and histological investigation of the postpartum bovine uterus in two estonian dairy herds.postpartum uterine infections, endometrial histology and resumption of ovarian activity in cows were studied in 2 estonian dairy herds with different herd sizes, milk yields and management systems. ten cows at farm a and 5 cows at farm b were studied in the experiment. all cows in the study had normal calving performance. endometrial biopsies for bacteriological and histological examinations were collected once a week starting on the second week postpartum and continuing for 7 weeks postpartum. ...19989926456
[immunity during ampicillin administration against a background of immunosuppression].the effect of 50 and 100 mg/kg doses of ampicillin, immunocompromised by cyclophan and azathioprine, on immunity of intact mice was studied. ampicillin did not change the number of antibody-forming cells (afc) and delayed hypersensitivity (dhs) in immunosuppression. it inhibited spontaneous oxidant metabolism of neutrophils and macrophages against the background of azathioprine. the therapeutic effect of ampicillin was maintained under such conditions. in intact animals the antibiotic did not ch ...19989929815
absence of escherichia coli, listeria monocytogenes, and klebsiella pneumoniae antigens within inflammatory bowel disease tissues.escherichia coli, listeria, and streptococcal antigens have been found in crohn's disease tissues. antibodies to klebsiella pneumoniae have been found in patients with inflammatory bowel disease and ankylosing spondylitis. the presence of these bacterial antigens in crohn's granulomas would be of aetiological interest, while their presence in ulcers alone would be more likely to indicate secondary infection.19989930068
[the bactericidal and bacteriostatic capacities of the amniotic fluid as a function of the time from membrane rupture]. 19989932006
[proteus syndrome and encephalo-cranio-cutaneous lipomatosis, are they the same hamartoma? apropos of a case]. 19989949594
antibacterial activity of human pleural fluid: alone and in combination with antibiotics.this study investigated the antibacterial activity of human pleural fluid (hpf) and its interaction with gentamicin (gm), meropenem (mrpm), ciprofloxacin (cpfx) and clarithromycin (cltm) against escherichia coli k-12, proteus rettgeri (sanelli) and staphylococcus aureus. minimal inhibitory concentrations or volumes, expressed as mic or volume percentage (miv, v/v%), were measured using a micro-dilution technique in microtiter plates. the antimicrobial activity of hpf combinations with antimicrob ...19989916908
[antibiotic sensitivity of bacteria isolated from the urine of children with urinary tract infections from 1986 to 1995].in adults and in children urinary system infections are mostly caused by gram-negative and rarely by gram-positive bacteria. of gram-negative bacteria the most frequent cause of infections are escherichia coli, klebsiella species, proteus mirabilis, pseudomonas aeruginosa, acinetobacter, serratia etc., and of gram-positive bacteria enterococcus, staphylococcus, streptococcus agalactiae. in rare cases the cause of infection may also be pneumococcus and haemophilus influenzae.19989921014
effect of sun-drying and chemical disinfection on contaminated cleaning cloth.in domestic environment, cross-contamination by contaminated cleaning cloth represents an infection hazard. could disinfectant use, detergent wash and rinse, and proper sun-drying be a panacea for cross-contamination? this is the fact-finding goal of this work. a sterilized towel (66.5 cm) was used to mop the eating tables of nnamdi azikiwe university cafeteria for 3 days, then aseptically collected and cut into six equal size, each for: estimation of total bacterial count; determination of the ...19989921089
thinking about bacterial populations as multicellular organisms.it has been a decade since multicellularity was proposed as a general bacterial trait. intercellular communication and multicellular coordination are now known to be widespread among prokaryotes and to affect multiple phenotypes. many different classes of signaling molecules have been identified in both gram-positive and gram-negative species. bacteria have sophisticated signal transduction networks for integrating intercellular signals with other information to make decisions about gene express ...19989891794
cellulitis due to escherichia coli in three immunocompromised subjects.in adults, cellulitis is usually caused by group a streptococci and staphylococcus aureus. however, in patients with underlying disease, it may be caused by other organisms, such as acinetobacter, clostridium septicum, enterobacter, haemophilus influenzae, proteus mirabilis or escherichia coli. we report three cases of cellulitis of the lower legs where e. coli was the causative bacterial organism. it is important to suspect e. coli as a causative organism if blistering cellulitis occurs, especi ...19989892960
characterization of plasmodium falciparum glutamate dehydrogenase-soluble antigen.the major aim of this study was to characterize a soluble plasmodium falciparum antigen from the plasma of malaria-infected humans and plasmodium falciparum culture supernatants, using immunoabsorbent techniques and western blotting. an mr 60-kda protein was isolated from the plasma of patients with plasmodium falciparum malaria by affinity chromatography using rabbit anti-proteus spp gdh(nadp+) serum as ligand. this protein, present in plasma of patients with acute plasmodium falciparum infecti ...19989876282
morganella morganii early onset sepsis. 19989877376
the effect of growth conditions on in vitro adherence, invasion, and naf expression by proteus mirabilis 7570.proteus mirabilis is a common cause of upper urinary tract infections. fimbriae-mediated adherence of this organism to urinary tract epithelium and invasion of host cells are factors thought to be important in its pathogenesis. we have assessed the effect of growth in serum, blood, and urine on the ability of p. mirabilis 7570 to adhere to and invade in vitro the cell line ej/28, derived from a human urinary tract tumour, and to express nonagglutinating fimbriae (naf). proteus mirabilis was capa ...19989851028
[emphysematous pyelonephritis].pyelonephritis is a frequent infection of variable severity. mortality is low since the era of antibiotics. emphysematous pyelonephritis is however a rare but life threatening form of kidney infection, which affect more frequently diabetic subjects, characterized by gas producing bacteria. these gaseous lesions can be localized in the renal parenchyma or in the perirenal or retroperitoneal space. diagnosis became easier with the advent of ct scan. treatment usually requires surgery and antibioti ...19989853041
febrile morbidity in severe and critical ovarian hyperstimulation syndrome: a multicentre study.the objective of this study was to define the incidence of febrile morbidity and its causes in severe and critical ovarian hyperstimulation syndrome (ohss). for this purpose, we reviewed the medical records of all ohss patients hospitalized in 16 out of 19 tertiary medical centres in israel between january 1987 and december 1996. febrile morbidity was defined as at least one episode of temperature rise above 38 degrees c lasting > or =24 h. a total of 2902 patients (3305 hospitalizations) with o ...19989853869
bacterial infection and endotoxin in female reproductive tract in rats: correlation with the developmental status of preimplantation embryos.during mammalian preimplantation development, a substantial numbers of embryos are believed to be lost for reasons that are unclear. using female rats, we investigated whether the developmental status of embryos is influenced by bacterial infection and endotoxin in the reproductive tract. from the vagina of cycling rats (n = 11), 21 bacterial isolates were identified; they were streptococcus faecalis (s. faecalis; 38%), escherichia coli (e. coli; 19%), acinetobactor calcoaceticus (a. calcoacetic ...19989854425
[infections in urology departments].the wide-spreading of intrahospital infections in urological departments on a nationwide scale are analyzed over the period 1982 through 1996. their epidemiological, etiological and nosological characterization is also defined. in this country over 6 per cent of all hospital infections occur in urological departments. annually, in bulgaria 5/100 discharged urological patients develop infection in hospital conditions. both gram-negative and gram-positive microorganisms are the etiological causing ...19989854940
microbiology of liver and spleen abscesses.to study the aerobic and anaerobic microbiology of liver and spleen abscesses and correlate the results with predisposing factors, potential causes and routes of infection, clinical and laboratory data of 48 patients with liver abscesses and 29 with spleen abscesses treated between 1970 and 1990 were reviewed retrospectively. in liver abscesses, a total of 116 isolates (2.4 isolates/specimen) was obtained; 43 were aerobic and facultative species (0.9 isolates/specimen) and 73 were anaerobic spec ...19989856643
[examination of selected biological properties for swarm cells in proteus mirabilis].certain biological properties, which generally are thought to play a role in the bacterial pathogenicity, were compared in short rods, swarm cells and penicillin-induced filaments of proteus mirabilis s1959. swarm cells of p. mirabilis s1959 weakly adhere to human uroepithelial cells and also do not penetrate l929 line of mouse fibroblasts. they do not show any cytotoxic activity, are poorly phagocytized by macrophages and granulocytes and start to divide by the end of the phagocytosis process. ...19989857610
[evaluation of in vitro susceptibility of hospital bacterial isolates to piperacillin and tazocin (piperacillin/tazobactam].due to increasing frequency of infections caused by pathogens that are resistant to beta-lactam antibiotics, combinations of such antibiotics and beta-lactamase inhibitors were introduced into therapy in last few years. tazobactam is the most potent beta-lactamase inhibitor. the purpose of the study was to evaluate in vitro susceptibility to piperacillin and piperacillin with tazobactam of 256 isolates cultured from biological samples obtained from 203 patients. the biological materials obtained ...19989857612
serological studies of an acid-labile o-polysaccharide of proteus vulgaris ox19 lipopolysaccharide using human and rabbit antibodies.in a weil-felix test, sera from patients infected with rickettsia sp. agglutinate proteus ox types of bacteria and proteus lipopolysaccharide (lps) are responsible for the cross-reaction. data on the character of lps of one of the ox group strains, proteus vulgaris ox19, are contradictory, and it remained unclear whether it has an o-polysaccharide (ops) and is thus lps of the smooth type (s) or not (rough-type lps). our studies showed that p. vulgaris ox19 (strain pzh-24) produces a smooth-type ...19989858461
[carbohydrate adaptive substance for prophylaxis of immune disorders and correction of dysbacterioses].effects of a new preparation, adaptokhit, on the intestinal microbiocenosis and some parameters of immune resistance were studied in macaque rhesus during 13-day hypokinesia. experimental animals were given adaptokhit as an alimentary supplement preventing dysbiotic consequences of the microecologic unbalance due to motor restraint. results of the investigation showed certain distinctions in the intestinal microflora in the experimental and control animals as early as on day three of the experim ...19989858982
[the antibacterial properties of the intestinal microflora of healthy calves].antibacterial properties of bacteria of genera escherichia, enterococcus, bacillus, lactobacillus, bifidobacterium isolated from different parts of gastro-intestinal tract of the healthy milk-fed and weanling calves were studied against test-bacteria, microorganisms of the escherichia genus with and without pathogenic factors as well as the genera klebsiella, enterobacter, proteus, salmonella, staphylococcus representatives that had been isolated from healthy and sick calves. certain tolerance w ...19989859643
[the characteristics of the causative agents of suppurative-inflammatory complications in hemophiliacs].morphological-cultural and physiological-biochemical properties of 24 strains of microorganisms agents of pyo-inflammatory complications of different localization in patients with hemophilia have been studied. microorganisms strains presented by the following species: staphylococcus aureus, s. epidermidis, s. saprophyticus, proteus vulgaris, p. morganii, hafnia alvei, serratia marcescens, have been identified. it was found out that in monoculture staphylococci prove to be the leading etiological ...19989859645
a six-domain structural model for escherichia coli translation initiation factor if2. characterisation of twelve surface epitopes.the escherichia coli translation initiation factor if2 is a 97 kda protein which interacts with the initiator fmet-trnafmet, gtp and the ribosomal subunits during initiation of protein biosynthesis. for structural and functional investigations of the factor, we have raised and characterised monoclonal antibodies against e. coli if2. twelve epitopes have been localised at the surface of the protein molecule by three different methods: interactions of the monoclonal antibodies with nested deletion ...19989861457
diversity among clinical isolates of proteus penneri detected by random amplified polymorphic dna analysis.dna of thirteen haemolytic proteus penneri strains of clinical origin, all producing calcium dependent haemolysin and having been derived from four european countries was examined for plasmid profile, and outer membrane protein profile, by random amplified polymorphic dna-pcr (rapd-pcr) method, and digestions with restriction endonucleases were performed. all strains contained two large plasmids of approximately 60 and 70 kilobase pairs (kb). in addition, four strains contained a small plasmid o ...19989861679
[hyperammonemia in hydronephrosis].a man aged 81 with bilateral hydronephrosis presented with lethargy deepening into coma caused by hyperammonaemia. the hyperammonaemia was most likely caused by excessive absorption of ammonia produced by proteus mirabilis in the obstructed and dilated urinary tract. the patient died within a few hours.19989864539
characterization of myosin heavy chain and its gene in amoeba proteus.monoclonal antibodies against the myosin heavy chain of amoeba proteus were obtained and used to localize myosin inside amoebae and to clone cdnas encoding myosin. myosin was found throughout the amoeba cytoplasm but was more concentrated in the ectoplasmic regions as determined by indirect immunofluorescence microscopy. in symbiont-bearing xd amoebae, myosin was also found on the symbiosome membranes, as checked by indirect immunofluorescence microscopy and by immunoelectron microscopy. the ope ...19989864850
[common antigenicity of lipopolysaccharides between rickettsiae and proteus in the weil-felix test]. 19989865067
[transient pseudohypoaldosteronism secondary to congenital malformation pathology of the urinary tract (valves of the posterior urethra): a report of a case with elements of a physiopathological nature].a case of secondary transient pseudohypoaldosteronism (pha) in a three-month-old infant who had arrived in emergency conditions is reported. clinically the patient presented dehydration associated with salt loss, metabolic acidosis, severe hyperkalemia, markedly elevated levels both of plasma renin activity and aldosterone concentration. biochemical and instrumental tests and clinical evolution have given reason to consider it as a case of transient pha, secondary to an obstructive uropathy (puv ...19989866855
characterisation of cmy-4, an ampc-type plasmid-mediated beta-lactamase in a tunisian clinical isolate of proteus mirabilis.a strain of proteus mirabilis resistant to beta-lactams, including cefoxitin, was isolated from the urine of a woman from tunisia. its antibiotic susceptibility pattern and that of the escherichia coli transconjugant suggested the presence of an ampc-type beta-lactamase. two bands of beta-lactamase activity (pi 5.4 and 9.2) were detected by isoelectric focusing. the nucleotide sequence of the gene encoding the ampc-type enzyme was determined. the deduced amino acid sequence was 98-99% identical ...19989868767
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