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[surgical policy in false aneurysm of proximal anastomosis secondary to reconstruction of aortofemoral segment].the results of surgical treatment of 10 patients with a false aneurysm of the proximal anastomosis are analyzed. an average time for development of a false aneurysm made up 57.2 months. fluorlon-lavsan vascular woven prosthesis of russian produce was used in primary reconstruction. bacteriological examination of operation material was carried out in 9 patients. 8 cases revealed the growth: of staph. aureus (4) epidermal staphylococcus flora (3) and proteus vulg. (1). the authors suggest that sur ...199910626379
stimulatory effects of endotoxin on the platelet secretory process.the effects of the lipopolysaccharides (lps) of proteus mirabilis (smooth and rough types), differing significantly in their composition on the release of compounds stored in specific platelet granules, were studied. there are two main types of secretory granules in blood platelets. dense granules contain adenine nucleotides, and in alpha-granules different proteins are stored. the lps were found to cause a dose-dependent release of proteins and adenine nucleotides. in the extracellular medium l ...199910624010
isolation and antibacterial activity of phenylpropanoid derivatives from ballota nigra.in addition to the previously isolated phenylpropanoid glycosides verbascoside 1, forsythoside b 2, arenarioside 3 and ballotetroside 4, another four compounds were isolated from generative aerial parts of ballota nigra: three phenylpropanoid glycosides, alyssonoside 5, lavandulifolioside 6 and angoroside a 7 and a non-glycosidic derivative (+)-(e)-caffeoyl-l-malic acid 8. the antibacterial activity of the five major compounds (1-4 and 8) was tested against gram-positive and gram-negative bacter ...199910619384
bacterial populations contaminating the upper gut in patients with small intestinal bacterial overgrowth syndrome.small intestinal bacterial overgrowth syndrome (sibos) is characterized by an abnormally high bacterial population level in the upper gut, exceeding 10(5) organisms/ml (5 log colony-forming unit (cfu)/ml). to understand its origin and select an appropriate antibiotic treatment, we have analyzed the bacterial populations contaminating the upper gut in sibos patients.199910235214
proteus syndrome: craniofacial and cerebral mri.the proteus syndrome is a rare hamartoneoplastic syndrome that may affect the brain, skull, and extracranial head and neck. we present a case with severe, characteristic findings. brain abnormalities are not common in proteus syndrome; when present, hemimegalencephaly and migrational disorders are typically seen, commonly with an associated seizure disorder. maxillary and mandibular dysmorphism may occur, including unilateral condylar hyperplasia. subcutaneous fatty, fibrous, lymphangiomatous ma ...199910602859
[erythrocytic glucose-6-phosphate dehydrogenase deficiency, hemolysis and diabetic ketoacidosis]. 199910603677
comparative evaluation of five chromogenic media for detection, enumeration and identification of urinary tract pathogens.five chromogenic agar plates--cps id2 medium (biomérieux, france), chromagar orientation medium (becton dickinson, france), uriselect3 medium (sanofi diagnostics pasteur, france), rainbow agar uti medium (biolog, usa) and chromogenic uti medium (oxoid, germany)--for the detection, enumeration and direct identification of urinary tract pathogens were compared using 443 urine specimens at two hospital laboratories. the enumeration of microorganisms was consistent on the five media for 403 of the 4 ...199910614954
ability of proteus mirabilis to swarm over urethral catheters.the aim of this study was to investigate the ability of proteus mirabilis to swarm over various types of urinary catheters. the test strain was found to swarm over catheters for distances of up to 10 cm within 24 h. migration was significantly more rapid over hydrogel-coated latex catheters than over all-silicone or silicone-coated latex catheters. scanning electron micrographs revealed discrete rafts of typically elongated swarmer cells on catheter surfaces. migration of swarmers along catheter ...199910357056
growth, cellular differentiation and virulence factor expression by proteus mirabilis in vitro and in vivo.a uropathogenic strain of proteus mirabilis was grown in vitro in human and mouse urine and brain-heart infusion broth (bhib) and in vivo in subcutaneous open chambers (soc) in mice, intraperitoneal diffusion chambers (ipc) in rats and by ascending urinary tract infection in mice in order to compare growth pattern, cellular differentiation and expression of virulence factors. although the growth rate was slower in vivo than in vitro, the extent of growth was similar after 24 h. pr mirabilis diff ...199910359301
proteus syndrome: diagnostic criteria, differential diagnosis, and patient evaluation.proteus syndrome is a complex disorder comprising malformations and overgrowth of multiple tissues. the disorder is highly variable and appears to affect patients in a mosaic manner. this intrinsic variability has led to diagnostic confusion associated with a dearth of longitudinal data on the natural history of proteus syndrome. to clarify some of these issues, a workshop on proteus syndrome was held in march 1998 at the national institutes of health, and participants developed recommendations ...199910360391
zapa, the iga-degrading metalloprotease of proteus mirabilis, is a virulence factor expressed specifically in swarmer cells.the iga-degrading metalloprotease, zapa, of the urinary tract pathogen proteus mirabilis is co-ordinately expressed along with other proteins and virulence factors during swarmer cell differentiation. in this communication, we have used zapa to monitor iga protease expression during the differentiation of vegetative swimmer cells to fully differentiated swarmer cells. northern blot analysis of wild-type cells and beta-galactosidase measurements using a zapa:lacz fusion strain indicate that zapa ...199910361285
antimicrobial activity of wedelia trilobata crude extracts.a biological screening of activity against gram-positive and gram-negative bacteria, yeasts, and fungi of crude extracts from wedelia trilobata is reported. the n-hexane extract showed antibacterial activity against bacillus subtilis, mycobacterium smegmatis, staphylococcus aureus, and staphylococcus epidermidis (gram-positive bacteria); along with proteus vulgaris, pseudomonas aeruginosa, salmonella group c, salmonella paratyphi, and shigella sonnei (gram-negative bacteria). the ethyl acetate e ...199910374253
[the role of phase contrast microscopy in the evaluation of cervix neck and vaginal biocenosis].the aim of our study was to evaluate and compare the possibility of germ identification in cervico-vaginal infections of different origin by ph cytology and classic fixated and stained (h-e) smears. we identified lactobacillus vag., gardnerella vag., short bacilli (escherichia coli, proteus, klebsiella), mixed coccus flora (diplococci, small and large cocci), trichomonas vag., and candidia alb. in cervico-vaginal smears. in endocervical, mature metaplastic or parabasal cells the elementary and c ...199910375921
the structure of the colony migration factor from pathogenic proteus mirabilis. a capsular polysaccharide that facilitates swarming.swarming by proteus mirabilis is characterized by cycles of rapid and coordinated population migration across surfaces following differentiation of vegetative cells into elongated hyperflagellated swarm cells. it has been shown that surface colony expansion by the swarm cell population is facilitated by a colony migration factor (cmf), a capsular polysaccharide (cps) that also contributes to the uropathogenicity of p. mirabilis (gygi, d., rahman, m. m., lai, h.-c., carlson, r., guard-petter, j., ...199910438465
flavone and xanthone derivatives related to fluoroquinolones.a number of flavone and xanthone derivatives bearing some characteristic features of fluoroquinolones such as the fluorine atom and an ortho piperazine ring are described. the new compounds have been tested for possible cytotoxic and antimicrobial activities. cytotoxicity of both groups of compounds is rather poor, while the antibacterial activity is restricted to xanthones.199910443020
antibacterial effects of lactoferricin b.the antimicrobial peptide, lactoferricin, can be generated upon gastric pepsin cleavage of lactoferrin. we have examined the inhibitory efficacy of lactoferricin of bovine origin (lf-cin b) on escherichia coli, proteus mirabilis and staphylococcus aureus with or without a cell wall. we found that spheroplasts and protoplasts had a lower mic than their counterparts with a cell wall. we also compared the efficacies of lf-cin b (17-31) made of all l-amino acids and all d-amino acids. the peptide ma ...199910447329
molecular characterization of the genera proteus, morganella, and providencia by ribotyping.the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ...199910449462
thanos syndrome does not exist. 199910449640
impact of use of multiple antimicrobials on changes in susceptibility of gram-negative aerobes.evaluation of antimicrobial usage vs. susceptibility relationships typically involves single agents. however, susceptibility profiles may be affected by multiple drugs. from 1992 through 1996, we studied relationships between drug usage and the susceptibility (only susceptibility rates of > or = 70%) of acinetobacter anitratus (baumannii), enterobacter aerogenes, escherichia coli, enterobacter cloacae, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, and serratia marcescens to 2 ...199910452628
evaluation of the antimicrobial potency of tannins and related compounds using the microdilution broth method.the antimicrobial activity of a total of 27 tannins and related compounds was evaluated against 8 microorganisms, including 2 gram-positive (bacillus subtilis, staphylococcus aureus), 4 gram-negative bacteria (escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis), and 2 yeasts (candida albicans, cryptococcus neoformans). the compounds tested were generally found to possess only weak to moderate antibacterial, but fairly high anticryptococcal activities. attention is ...199910454901
microbial decolorization of azo dyes by proteus mirabilis.a bacterium identified as proteus mirabilis was isolated from acclimated sludge from a dyeing wastewater treatment plant. this strain rapidly decolorized a deep red azo dye solution (red rbn). features of the decolorizing process related to biodegradation and biosorption were also studied. although p. mirabilis displayed good growth in shake culture, color removal was best in anoxic static cultures. for color removal, the optimal ph and temperature were 6.5-7.5 and 30-35 degrees c, respectively. ...199910455502
structures of the o-specific polysaccharides and a serological cross-reactivity of the lipopolysaccharides of proteus mirabilis o24 and o29.strains of proteus mirabilis belonging to serogroups o24 and o29 are frequent in clinical specimens. anti-p. mirabilis o24 serum cross-reacted with the lipopolysaccharide (lps) of p. mirabilis o29 and vice versa. the structures of the o-specific polysaccharides (opss, o-antigens) of both lpss were established using sugar analysis and one- and two-dimensional 1h- and 13c-nmr spectroscopy and found to be different. sds-page and western immunoblotting suggested that the serological cross-reactivity ...199910456314
proteus syndrome: a possible case associated to precocious puberty.we report a boy with possible proteus syndrome and precocious puberty. this appears to be the first report of this association.199910457862
antimicrobial resistance patterns in urinary isolates from nursing home residents. fifteen years of data reviewed.the antibiotic resistance patterns of gram-negative bacteria isolated from nursing home patients between 1983 and 1997 were analysed. escherichia coli was the most prevalent isolate (48%) followed by proteus spp. (26%) and other enterobacteriaceae (20%). during the study period, the susceptibility of e. coli decreased for co-trimoxazole (79% to 62%), increased for nitrofurantoin (79% to 91%) and remained unchanged for amoxycillin (41%). susceptibility to norfloxacin, available from 1990, decreas ...199910459818
antibacterial activity of ocimum gratissimum l. essential oil.the essential oil (eo) of ocimum gratissimum inhibited staphylococcus aureus at a concentration of 0.75 mg/ml. the minimal inhibitory concentrations (mics) for shigella flexineri, salmonella enteritidis, escherichia coli, klebsiella sp., and proteus mirabilis were at concentrations ranging from 3 to 12 microg/ml. the endpoint was not reached for pseudomonas aeruginosa (>=24 mg/ml). the mics of the reference drugs used in this study were similar to those presented in other reports. the minimum ba ...199910464416
microbes and sequestered substances as mechanisms for disease: bartonella and l-forms as common global etiological agents.the pathogenicity of microbes may be determined by substances sequestered from blood and bound to their constituent lipid. the brain may not perceive substances sequestered by microbes, to interfere with control to maintain normal levels. pathological conditions can be induced as organisms exposed to antimicrobial substances/conditions and/or deprived of nutrients essential to cell wall synthesis, disintegrate to free lipid-bound compounds and produce l-forms that can deplete nutrients as they r ...199910465665
in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group.we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ...199910467641
investigation of influence of proteus mirabilis lps polysaccharide part on the murine immune cells activation.the biological activities were investigated of proteus mirabilis lipopolysaccharides (lpss) and their fragments, namely, the induction of nitric oxide (no) and interleukin 1 (il-1) synthesis by murine macrophages and the proliferation of murine spleen cells. the o-specific polysaccharide (f1), core oligosaccharide (f2) and lipid a were as effective as intact lps in stimulating murine macrophages to produce no. il-1 synthesis was also induced by all studied types of endotoxins (s, ra, re) and par ...199910470447
paediatric nosocomial urinary tract infection at a regional hospital.from a total of 26,603 admissions to the paediatric wards, 1360 paediatric nosocomial urinary tract infections (pnuti) were identified during a 5-year retrospective chart review at the sfgh. the ages ranged from 3 days to 13 years, with 46% boys and 54% girls. the highest rates of pnuti per service per 100 admissions were seen in the nursery (11.28) followed by paediatric surgery (2.89) and paediatric medicine (2.86). although the greatest number of pnuti occurred in the nursery, comparison betw ...199910481961
proteus mirabilis osteomyelitis of the ribs in a girl with sickle cell anaemia. 199910485315
canine distemper virus infection in binturongs (arctictis binturong).two binturongs (arctictis binturong) kept in outdoor exhibits at everland zoological gardens in korea died within 10 days of the onset of clinical signs that included depression, dyspnoea, diarrhoea and convulsions. on necropsy, the significant gross findings were limited to the lungs and the gastrointestinal tract. proteus vulgaris was isolated from the lung of one animal. histopathologically, diffuse severe bronchointerstitial pneumonia with secondary bacterial infection was noted in the lungs ...199910486167
the facilitating effect of cigarette smoke on the colonization of instilled bacteria into the tracheal lumen in rats and the improving influence of supplementary vitamin e on this process.the aim of the present study was to investigate the direct influence of cigarette smoke on the bacterial colonization of the lower respiratory tract and the effect of supplementary vitamin e on the colonization of instilled bacteria into the trachea of rats that do not have the chronic airway pathology associated with smoking.199910489666
[development of a vaccine for treatment of urinary tract infections].receiving uroinfections are predominantly treated with antibiotics and chemotherapeutics. an effective alternative treatment can be immunotherapy with inactivated bacteria. the authors have focused on the development of polybacterial vaccine with both specific and nonspecific immunostimulatory activity. the vaccine contains killed bacteria which most frequently occur as an etiological agent in urinary tract infections. determining for the selection of particular strains for the vaccine preparati ...199910493005
[clinical success of treatment of chronic otitis media using topical and peroral administration of ofloxacin].the paper presents results of the treatment of 32 patients (35 ears) with chronic otitis media and long lasting purulent otorrhea with local and oral ofloxacin. the patients were divided in two groups. group a included 17 patients (49%), with previous middle ear surgery. group b comprised 15 patients (18 ears, 51%) treated with local and systemic antimicrobial drugs. ofloxacin was administered after taking the swab sample, and the duration of treatment was the same in all patients. the most freq ...199910494153
genomic subtractive hybridization and selective capture of transcribed sequences identify a novel salmonella typhimurium fimbrial operon and putative transcriptional regulator that are absent from the salmonella typhi genome.salmonella typhi, the etiologic agent of typhoid fever, is adapted to the human host and unable to infect nonprimate species. the genetic basis for host specificity in s. typhi is unknown. the avirulence of s. typhi in animal hosts may result from a lack of genes present in the broad-host-range pathogen salmonella typhimurium. genomic subtractive hybridization was successfully employed to isolate s. typhimurium genomic sequences which are absent from the s. typhi genome. these genomic subtracted ...199910496884
quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from pseudomonas aeruginosa and other gram-negative bacteria.in cell-free pseudomonas aeruginosa culture supernatants, we identified two compounds capable of activating an n-acylhomoserine lactone (ahl) biosensor. mass spectrometry and nmr spectroscopy revealed that these compounds were not ahls but the diketopiperazines (dkps), cyclo(deltaala-l-val) and cyclo(l-pro-l-tyr) respectively. these compounds were also found in cell-free supernatants from proteus mirabilis, citrobacter freundii and enterobacter agglomerans [cyclo(deltaala-l-val) only]. although ...199910510239
antibiotic sensitivity of bacteria associated with community-acquired urinary tract infection in britain.twelve laboratories in different parts of britain each supplied approximately 80 consecutive urinary bacterial isolates from community patients. all strains were identified by a central laboratory, where sensitivity to a variety of orally administered antimicrobials was determined by microtitre broth dilution. 65.1% of isolates were escherichia coli, 23.4% 'coliforms' other than e. coli, 4.6% proteus and morganella spp., 1.8% pseudomonas spp., 2.4% enterococci, 0.7% group b streptococci, 1.5% co ...199910511403
association of rickettsia helvetica with chronic perimyocarditis in sudden cardiac death.rickettsia helvetica is the only non-imported rickettsia found in scandinavia. it was first detected in ixodes ricinus ticks, but has never been linked to human disease. we studied two young swedish men who died of sudden cardiac failure during exercise, and who showed signs of perimyocarditis similar to those described in rickettsial disease.199910513711
in vitro antibacterial activity of fk041, a new orally active cephalosporin.the in vitro activity of fk041, a new orally active cephem antibiotic, against a wide variety of clinical isolates of bacteria was investigated and compared with those of cefdinir (cfdn) and cefditoren (cdtr). fk041 exhibited broad spectrum activity against reference strains of gram-positive and gram-negative aerobes and anaerobes. fk041 was active against clinical isolates of gram-positive organisms except enterococcus faecalis with mic90s less than 1.56 microg/ml. fk041 was more active than cf ...199910513845
structure of a new acidic o-antigen of proteus vulgaris o22 containing o-acetylated 3-acetamido-3,6-dideoxy-d-glucose.the acidic o-specific polysaccharide of proteus vulgaris o22 was studied using 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments, and the following structure for the branched pentasaccharide repeating unit was established: [sequence: see text] where qui3nac is 3-acetamido-3,6-dideoxyglucose, o-acetylation of quinac at position 4 is stoichiometric and at position 2 nonstoichiometric. serological relatio ...199910515053
structure and cross-reactivity of the o-antigen of proteus vulgaris o8.a high-molecular-mass o-specific polysaccharide was obtained by mild acid degradation of proteus vulgaris o8 lipopolysaccharide followed by gel permeation chromatography. studies of the polysaccharide by sugar and methylation analyses and 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments, demonstrated the presence of a tetrasaccharide repeating unit having the following structure: [sequence: see text] ...199910515057
abolition of swarming of proteus. 199910515741
the use of chromagar orientation as a primary isolation medium with presumptive identification for the routine screening of urine specimens.the aim of the study was to compare the use of a novel differential culture medium chromagar, for both primary isolation and presumptive identificaton, with the method currently used in our laboratory for screening mid-stream-urine samples (msu). routine methods (rm) included blotting paper imprinting of all specimens and additional quantitative culture on cysteine lactose electrolyte-deficient agar (cled) for selected samples together with microbact 12e for further identification. the chromagar ...199910519322
the structure of the core part of proteus mirabilis o27 lipopolysaccharide with a new type of glycosidic linkage.the structural assignment of the intact lipopolysaccharide core from proteus mirabilis o27 has been completed based on a combination of chemical degradation studies, nmr spectroscopy and es-ms spectroscopy. the overall core structure is as follows: [formula: see text] where all sugars are in the pyranose form except the n-acetylglycosamine residue, hep refers to l-glycero-alpha-d-manno-heptopyranose and alpha-ddhep to d-glycero-alpha-d-manno-heptopyranose. bold italics indicate non-stoichiometri ...199910520257
structure of an acidic o-specific polysaccharide of proteus mirabilis o5.the following structure of the o-specific polysaccharide of proteus mirabilis o5 was established by 1h and 13c nmr spectroscopy at 500 mhz, including two-dimensional cosy, tocsy, noesy, and h-detected 1h, 13c heteronuclear multiple-quantum coherence (hmqc) experiments: [formula: see text] where o-acetylation of alpha-d-glcnac at both positions is nonstoichiometric.199910520266
molecular mechanisms of interaction of rabbit cap18 with outer membranes of gram-negative bacteria.the mechanism of interaction of the cationic antimicrobial protein (18 kda), cap18, with the outer membrane of gram-negative bacteria was investigated applying transmission electron microscopy and voltage-clamp techniques on artificial planar bilayer membranes. electron micrographs of bacterial cells exposed to cap18 showed damage to the outer membrane of the sensitive escherichia coli strains f515 and atcc 11775, whereas the membrane of the resistant proteus mirabilis strain r45 remained intact ...199910521271
evolutionary changes reflected by the cellular amino acid composition.comparison of the amino acid composition of cell-proteins using 17 amino acids has been used to investigate the biological evolution of organisms such as bacteria, blue-green alga, green alga, fungi, slime mold, protozoa and vertebrates. the degree of difference in the amino acid ratios between any two groups reflects the degree of divergency in biological evolution. the amino acid composition of the gram-negative bacteria (escherichia coli, klebsiella, proteus, and vibrio alginolyticus) was ide ...199910524278
proteus mirabilis viability after lithotripsy of struvite calculi.we tested the hypotheses that proteus mirabilis viability of struvite calculi differs after exposure to different lithotripsy modalities and that the photothermal mechanism of holmium:yag lithotripsy is antibacterial.199910524893
characteristics of pathogens causing urinary tract infections in hospitals in north america: results from the sentry antimicrobial surveillance program, 1997.urinary tract infection (uti) is common and involves pathogens with changing susceptibility patterns. the sentry antimicrobial surveillance program evaluates international pathogen incidence patterns to detect and manage the emergence of resistant strains. we describe the antimicrobial resistance patterns among 1617 pathogens recovered from utis during the third-quarter of 1997 in north america (united states and canada), as part of this worldwide program. the isolates were tested against more t ...199910529882
identification and molecular analysis of the gene encoding rickettsia typhi hemolysin.rickettsia typhi, the causative agent of murine typhus, grows directly within the host cell cytoplasm, accumulating a large number of progeny, and eventually lyses the cells. typhus group rickettsiae (r. typhi and r. prowazekii) adhere to and lyse human and sheep erythrocytes. however, the molecular mechanism underlying erythrocyte lysis by r. typhi has not been defined. here we describe the cloning and nucleotide sequence analysis of the gene (tlyc) encoding a hemolysin from r. typhi. dna seque ...199910531273
a child with hemimegalencephaly, hemihypertrophy, macrocephaly, cutaneous vascular malformation, psychomotor retardation and intestinal lymphangiectasia--a diagnostic dilemma.although the clinical delineation of the majority of overgrowth syndromes is straightforward, we believe there is a subset of patients with overlapping features from a number of overgrowth syndromes. we report a patient with hemimegalencephaly, hemihypertrophy, macrocephaly, vascular lesions, psychomotor retardation and intestinal lymphangiectasia. the clinical history and findings posed a diagnostic dilemma as the features overlapped between several conditions, namely macrocephaly-cutis marmora ...199910532178
an exploration of the accuracy of an invasive method for measuring the volume of specimens of amoeba proteus.a method is described for measuring the volume of individual specimens of amoeba proteus which utilizes an easily constructed compressor to flatten the specimen to a known thickness. the microscopic image of the flattened specimen is captured on tape, digitized and analysed with the nih image software. the results from one specimen are given to illustrate the sources and magnitude of errors affecting these volume measurements.199910540260
diversity of tem mutants in proteus mirabilis.in a survey of resistance to amoxicillin among clinical isolates of proteus mirabilis, 10 tem-type beta-lactamases were characterized: (i) the well-known penicillinases tem-1 and tem-2, the extended-spectrum beta-lactamases (esbls) tem-3 and tem-24, and the inhibitor-resistant tem (irt) tem-44 and (ii) five novel enzymes, a penicillinase tem-57 similar to tem-1, an esbl tem-66 similar to tem-3, and three irts, tem-65, tem-73, and tem-74. the penicillinase tem-57 and the esbl tem-66 differed from ...199910543745
antimicrobial activities of several parts of pinus brutia, juniperus oxycedrus, abies cilicia, cedrus libani and pinus nigra.in this study, the antimicrobial activities of several parts of various trees grown in the kahramanmaraƟ region of turkey were investigated by the disc diffusion method. chloroform, acetone and methanol extracts of leaves, resins, barks, cones and fruits of pinus brutia ten., juniperus oxycedrus l., abies cilicia ant. & kotschy carr., cedrus libani a. rich. and pinus nigra arn. were prepared and tested against bacillus megaterium dsm 32, bacillus subtilis img 22, bacillus cereus fmc 19, escheric ...199910548751
minimal debridement in the treatment of fournier's gangrene.scrotal and perineal gangrene of the male genitalia was first described by fournier in 1883. treatment of the disease remains surgical despite the availability of modern antibiotics. many authors advocate a wide debridement of all tissue superficial to the involved fascial layers. we have reviewed our experience of using wide and minimal debridement as the surgical technique of choice. in our retrospective study, nine patients were diagnosed and treated over a 2-year period for fournier's gangre ...199910551751
the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae.the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ...199910555323
clostridium difficile toxins may augment bacterial penetration of intestinal epithelium.clostridium difficile can be recovered from many high-risk hospitalized patients receiving broad-spectrum antibiotic therapy. clostridium difficile toxins a and b have been associated with increased intestinal permeability in vitro and there is growing evidence that increased intestinal permeability may be a common mechanism whereby enteric bacteria penetrate the intestinal epithelium.199910555639
preseptal cellulitis secondary to proteus species: a case report and review.preseptal cellulitis is a serious ocular condition that--if left untreated--has the potential to cross the septal barrier, spread to the posterior orbit, and may result in fatal complications. because it is difficult to determine the pathogen responsible for any cellulitis without aspirating a culture sample, treatment is usually instituted by an assumption of the most common causative organisms, staphylococcus or streptococcus.199910561925
effect of thermal injury with pseudomonas aeruginosa infection on pulmonary and systemic bacterial clearance.despite improvements in burn wound care, infections, particularly pneumonia, remain a major hurdle to recovery from thermal injury. after burns, a variety of systemic immune and inflammatory changes contribute to the risk of infection. clinically, infection coupled with burn injury seems to adversely affect susceptibility to subsequent infection.199910568721
serum immunoglobulin response and protection from homologous challenge by proteus mirabilis in a mouse model of ascending urinary tract infection.we tested the hypothesis that experimental proteus mirabilis urinary tract infection in mice would protect against homologous bladder rechallenge. despite production of serum immunoglobulin g (igg) and igm (median titers of 1:320 and 1:80, respectively), vaccinated (infected and antibiotic-cured) mice did not show a decrease in mortality upon rechallenge; the survivors experienced only modest protection from infection (mean log(10) number of cfu of p. mirabilis nal(r) hi4320 per milliliter or gr ...199910569791
the structure of the core part of proteus vulgaris ox2 lipopolysaccharide.the identity of a novel structural component, an open-chain acetalic linkage, in the core part of the lipopolysaccharide (lps) from proteus vulgaris serotype ox2 has been determined by extensive nmr spectroscopic analysis of fragments isolated after mild acid hydrolysis of the intact lps. the open-chain n-acetylgalactosamine fragment is substituted in the 4-position by non-stoichiometric amounts of a beta-galactopyranose residue and the overall structure of the core is as follows: [formula: see ...199910573861
[advances and prospects of a combined therapy of acute radiation injury in experiments].we present the results of a long-standing experimental development of ways and means for acute radiation sickness treatment that have been authorized for application in medicine and are mostly aimed at large-scale accidental injuries. the paper describes means for early treatment (prodigiosan, desoxynate, typhoid vaccine, proteus vaccine), a myeolopoiesis stimulant (estradiol dipropionate), a detoxication procedure (hemosorption), substitution therapy with bone marrow cells and peripheral blood ...199910576028
cartilage degradation by hyaluronate lyase and chondroitin abc lyase: a maldi-tof mass spectrometric study.matrix-assisted laser desorption ionization and time-of-flight mass spectrometry (maldi-tof ms) has been used to investigate degradation products of two selected polysaccharides of cartilage (chondroitin sulfate and hyaluronic acid). testicular hyaluronate lyase and chondroitin abc lyase were used for enzymic digestion of both polysaccharides as well as of cartilage specimens. polysaccharide solutions and cartilage supernatants were assayed by positive and negative maldi-tof ms. especially chond ...199910576924
evaluation of the in vitro antimicrobial activity of cefepime compared to other broad-spectrum beta-lactams tested against recent clinical isolates from 10 chinese hospitals. chinese antimicrobial resistance study group.a surveillance study was initiated in china in 1998 in which 10 medical centers participated. the susceptibility profiles of 996 commonly occurring pathogens belonging to 10 different species groups were tested by the etest (ab biodisk, solna, sweden) against six broad-spectrum beta-lactam antimicrobial agents (cefepime, ceftazidime, ceftriaxone, imipenem, cefoperazone/sulbactam and piperacillin or oxacillin). quality control was closely monitored and cefepime- and/or imipenem-resistant enteroba ...199910579094
molecular recognition of synthetic siderophore analogues: a study with receptor-deficient and fhu(a-b) deletion mutants of escherichia coli.the biological activity of six synthetic siderophore analogues (two dihydroxamates, two trihydroxamates, one tetrahydroxamate and one 3-hydroxy-4(1h)pyridinone) has been studied in escherichia coli, morganella morganii 13 and proteus mirabilis 8993 strains by using growth promotion tests. various transport-deficient mutants of e. coli were used to study the route of entry into gram-negative bacteria. the results indicated that the synthetic hydroxamate compounds are transported via fhu-mediated ...199910581683
nephrobronchial fistula secondary to xantogranulomatous pyelonephritis.we report a case of staghorn nephrolithiasis that evolved into xanthogranulomatous pyelonephritis with perinephric abscess, nephrobronchial fistula, and lung abscess. the patient was an intravenous drug abuser who tested positive for human immunodeficiency virus, without evidence of acquired immunodeficiency syndrome. he presented with a 2-month history of untreated repeated episodes of left flank pain and hyperpyrexia. treatment involved left nephrectomy, debridement of abscess, tube drainage, ...199910585069
gram-negative bacillary meningitis in adult post-neurosurgical patients.to assess the clinical features and therapeutic outcomes of gram-negative bacillary meningitis (gnbm) in adult postneurosurgical patients.199910595761
genomic rearrangements in the flagellin genes of proteus mirabilis.molecular analyses have revealed that proteus mirabilis possesses two genes, flaa and flab, that are homologous to each other and to flagellin genes of many other species. both swimmer and swarmer cells transcribe flaa, but not flab. flaa- mutants are non-motile and do not differentiate showing the essential role of flaa in swarmer cell differentiation and behaviour. at a low frequency, motile, differentiation-proficient revertants have been found in flaa-populations. these revertants produce an ...199910027983
unaccountable severe hypercalcemia in a patient treated for hypoparathyroidism with dihydrotachysterol.this report describes a forty-seven-year-old female patient with a complex medical history. she was suffering from an unspecified interstitial lung disease, papillary thyroid carcinoma which had been treated, hypoparathyroidism after thyroidectomy for which she was receiving dihydrotachysterol and calcium, and atrial fibrillation and congestive heart failure as a result of mitral stenosis. shortly after mitral valve replacement she developed a severe hypercalcemia (serum calcium 5.95 mmol/l) dur ...199910048291
antimicrobial activities of synthetic bismuth compounds against clostridium difficile.clostridium difficile is a major nosocomial pathogen responsible for pseudomembranous colitis and many cases of antibiotic-associated diarrhea. because of potential relapse of disease with current antimicrobial therapy protocols, there is a need for additional and/or alternative antimicrobial agents for the treatment of disease caused by c. difficile. we have synthesized a systematic series of 14 structurally simple bismuth compounds and assessed their biological activities against c. difficile ...199910049270
role of ribosome release in regulation of tna operon expression in escherichia coli.expression of the degradative tryptophanase (tna) operon of escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. in cultures growing in the absence of added tryptophan, transcription of the structural genes of the tna operon is limited by rho-dependent transcription termination in the leader region of the operon. tryptophan induction prevents this rho-dependent termination, and requires in-frame translation of a 24-residue leader peptide co ...199910049385
an escherichia coli strain with all chromosomal rrna operons inactivated: complete exchange of rrna genes between bacteria.current global phylogenies are built predominantly on rrna sequences. however, an experimental system for studying the evolution of rrna is not readily available, mainly because the rrna genes are highly repeated in most experimental organisms. we have constructed an escherichia coli strain in which all seven chromosomal rrna operons are inactivated by deletions spanning the 16s and 23s coding regions. a single e. coli rrna operon carried by a multicopy plasmid supplies 16s and 23s rrna to the c ...199910051579
increasing prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in women.guidelines for the management of acute uncomplicated cystitis in women that recommend empirical therapy in properly selected patients rely on the predictability of the agents causing cystitis and knowledge of their antimicrobial susceptibility patterns.199910052444
pylephlebitis associated with appendicitis.pylephlebitis usually occurs secondary to infection in the region drained by the portal venous system. a most common antesecent focus of infection is diverticulitis and the most common blood isolate is e. coli (54%), followed by proteus mirabilis (23%). overall mortality is 32% and most of the patients who had died had severe sepsis prior to the initiation of antibiotic therapy. we describe a case of pylephlebitis which had appendicitis and consequent septic thrombosis of the portal vein and its ...199910063317
penetration of ciprofloxacin, norfloxacin and ofloxacin into the aqueous humor using different topical application modes.a prospective study was undertaken to determine the transcorneal penetration of three topically applied fluoroquinolones into aqueous humor.19999987622
aerobic and anaerobic microbiology of surgical-site infection following spinal fusion.the aerobic and anaerobic microbiology of surgical-site infections (ssi) following spinal fusion was retrospectively studied. this was done by reviewing the clinical and microbiological records at the naval hospital in bethesda, md., from 1980 to 1992. aspirates of pus from 25 infection sites showed bacterial growth. aerobic bacteria only were recovered from 9 (36%) specimens, anaerobic bacteria only were recovered from 4 (16%), and mixed aerobic and anaerobic bacteria were recovered from 12 (48 ...19999986872
bacteria in lung tissue from an autopsy population of alcoholics.the retrieval of bacteria from the lungs postmortem was examined in a population of alcoholics who had a medico-legal autopsy performed. the results were compared with non-alcoholic controls. pneumococci were found more frequently in alcoholics, but in general there were no major differences. proteus mirabilis was detected in three out of five alcoholics with unascertainable cause of death. it is speculated whether this species may cause septicaemia in some alcoholics due to abnormal splanchnicu ...199910069022
cohort study of bacterial species causing urinary tract infection and urinary tract abnormalities in children. 199910082700
the isoflavone genistein inhibits internalization of enteric bacteria by cultured caco-2 and ht-29 enterocytes.the dietary isoflavone genistein is the focus of much research involving its role as a potential therapeutic agent in a variety of diseases, including cancer and heart disease. however, there is recent evidence that dietary genistein may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. to study the effects of genistein on bacterial adherence and internalization by confluent enterocytes, caco-2 and ht-29 enterocytes (cultivated for 15-18 d and 21-24 d, respectively) ...199910082767
[lumbar puncture in a pediatric emergency department: something more than a diagnostic technic].the aim of this study was to know the incidence of serious bacterial infections (sbi) in children without sepsis or intracranial infection in which spinal puncture (lp) was performed in an emergency department.199910083638
the penicillins.the penicillin family of antibiotics remains an important part of our antimicrobial armamentarium. in general, these agents have bactericidal activity, excellent distribution throughout the body, low toxicity, and efficacy against infections caused by susceptible bacteria. the initial introduction of aqueous penicillin g for treatment of streptococcal and staphylococcal infections was an important pharmacologic landmark. the emergence of penicillinase-producing staphylococcus aureus prompted the ...199910090000
functional analysis of the evolutionarily conserved proline 53 residue in proteus mirabilis glutathione transferase b1-1.the role of the evolutionarily conserved residue pro-53 in proteus mirabilis glutathione transferase b1-1 has been examined by replacing it with a serine residue using site-directed mutagenesis. the effect of the replacement on the activity, thermal stability and antibiotic binding capacity of the enzyme was examined. the results presented support the view that pro-53 participates in the maintenance of the proper conformation of the enzyme fold rather than playing a direct role in the catalytic ...199910094487
a novel membrane protein influencing cell shape and multicellular swarming of proteus mirabilis.swarming in proteus mirabilis is characterized by the coordinated surface migration of multicellular rafts of highly elongated, hyperflagellated swarm cells. we describe a transposon mutant, mns185, that was unable to swarm even though vegetative cells retained normal motility and the ability to differentiate into swarm cells. however, these elongated cells were irregularly curved and had variable diameters, suggesting that the migration defect results from the inability of these deformed swarm ...199910094676
[use of pefloxacin in the treatment of patients with purulent wounds of soft tissues].pefloxacin was used in the treatment of 25 patients with wound infection in a dose of 400 mg orally twice a day for 10-12 days. as the monotherapy it was applied to 15 patients. 7 patients with clinical signs of non-clostridial anaerobic infection were treated with pefloxacin in combination with intravenous metronidazole. pefloxacin was highly efficient in 96 per cent of the cases with extensive posttraumatic purulent wounds with and without bone affection, acute purulent wounds of the soft tiss ...199910095920
neurofibromatosis type 1.neurofibromatosis (nf) has perhaps been the most notorious of the neurocutaneous disorders. both quasimoto of victor hugo's the hunchback of notre dame and john merrick, known as the elephant man (who subsequently has been more properly classified as having proteus syndrome, a quite different disorder), are 2 infamous examples that have shaped many popular misconceptions about this disease. neurofibromatosis is now understood as a hamartomatous disorder on the basis of molecular genetic studies. ...199910190829
[the effect of erythromycin on immunity in immunodeficiency].the effect of 12.5 and 25 mg/kg doses of erythromycin on the immunity of intact mice and in cyclophosphan- and azathioprine-induced immunodeficiency was studied. erythromycin has no effect on the number of antibody-forming cells in the spleen of intact and immunocompromised with cyclophosphan animals. it increases their number against the background of azathioprine. immunosuppression may change the character of the effect of erythromycin on delayed hypersensitivity. erythromycin stimulates phago ...199910198768
identification of urer binding sites in the enterobacteriaceae plasmid-encoded and proteus mirabilis urease gene operons.the closely related proteus mirabilis and enterobacterlaceae plasmid-encoded urease genes are positively regulated by the arac-like transcriptional activator urer. in the presence of the effector molecule urea, urer promotes transcription of ured, the initial gene in the urease operon, and increases transcription of the divergently transcribed urer. here, we identify urer-specific binding sites in the urerp-uredp intergenic regions. recombinant urer (rurer) was expressed and purified, and gel sh ...199910200962
a comparison of teicoplanin versus cephradine plus metronidazole in the prophylaxis of post-operative infection in vascular surgery.a total of 272 patients were enrolled into this prospective, unblinded, randomized comparison of single-dose teicoplanin vs three doses of cephradine plus metronidazole as prophylaxis for vascular surgery at st james's and seacroft hospitals, leeds, uk. in all, 71.3% of patients (194/272) were enrolled at st james's university hospital. patients received either a single dose of teicoplanin, 6 mg/kg i.v., or cephradine, 1 g i.v. with metronidazole, 1 g rectally, at induction of anaesthesia follow ...199910204127
identification of protease and rpon-associated genes of uropathogenic proteus mirabilis by negative selection in a mouse model of ascending urinary tract infection.proteus mirabilis, a motile gram-negative bacterium, is a principal cause of urinary tract infections in patients with functional or anatomical abnormalities of the urinary tract or those with urinary catheters in place. thus far, virulence factors including urease, flagella, haemolysin, various fimbriae, iga protease and a deaminase have been characterized based on the phenotypic traits conferred by these proteins. in this study, an attempt was made to identify new virulence genes of p. mirabil ...199910206698
antibacterial activity of gentamicin and ciprofloxacin against gram-negative bacteria: interactions with pig and calf sera.the antibacterial activity of pig and calf serum and its ability to interact with gentamicin and ciprofloxacin were studied in vitro using escherichia coli k-12, proteus rettgeri (sanelli) and klebsiella pneumoniae atcc 10031. the antimicrobial activity of the above drugs, alone or in combination with serum, was investigated by the checkerboard method and expressed as the minimal inhibitory concentration (microg ml-1). pig serum (25%) with gentamicin had a synergistic antibacterial effect agains ...199910208763
amplification of proteus mirabilis chromosomal dna using the polymerase chain reaction.a proteus mirabilis-specific polymerase chain reaction (pcr) was developed and standardized. the origin of the primers was a recombinant clone that contained p. mirabilis-specific hind iii fragment dna of 3.5-kilobase pairs. based on the sequence data of p. mirabilis recombinant clone, two primers designated mmkap 1 and mmkap 2 were synthesized for use in the pcr. a p. mirabilis-specific 3.5-kb pair dna product was amplified by the primers from 18 strains of p. mirabilis, but not from other prot ...199910208804
synthesis and evaluation of 2 beta-oxyimino and alkenylpenicillanic acid sulfone derivatives as beta-lactamase inhibitors.the synthesis and in vitro synergies of 2 beta-alkenyl and oxyiminopenam sulfone derivatives are described. most of the compounds synthesized exhibited good inhibitory activities and synergistic antibacterial activities with piperacillin and ceftriaxone, respectively, against several beta-lactamase producing strains. particularly the 2 beta-alkenylpenam sulfone derivatives. 1e and 1g, showed good synergistic activity with ceftriaxone against citrobacter freundi nih 10018-68 and proteus vulgaris ...199910073138
elattoproteus syndrome: delineation of an inverse form of proteus syndrome.a 7-year-old boy had partial lipohypoplasia and patchy dermal hypoplasia involving large areas of his body. these areas of deficient growth were similar to those described in many cases of proteus syndrome. paradoxically, however, he had only few and rather mild lesions of disproportionate overgrowth. this unusual case is taken as a clue to postulate the elattoproteus syndrome, an inverse form of proteus syndrome. the paradoxical coexistence of hyperplastic and hypoplastic lesions may reflect a ...199910213042
structural and serological studies on the o-antigen of proteus mirabilis o14, a new polysaccharide containing 2-[(r)-1-carboxyethylamino]ethyl phosphate.an o-specific polysaccharide was obtained by mild acid degradation of proteus mirabilis o14 lipopolysaccharide (lps) and found to contain d-galactose, 2-acetamido-2-deoxy-d-glalactose, phosphate, n-(2-hydroxyethyl)-d-alanine (d-alaetn), and o-acetyl groups. studies of the initial and o-deacetylated polysaccharides using one- and two-dimensional 1h- and 13c-nmr spectroscopy, including cosy, tocsy, noesy, h-detected 1h,13c heteronuclear multiple-quantum coherence, and heteronuclear multiple-bond c ...199910215843
structure of the o-specific polysaccharide of a serologically separate strain proteus penneri 2 from a new proposed serogroup o66.o-specific polysaccharide chain of proteus penneri strain 2 lipopolysaccharide was studied by full and partial acid hydrolysis, smith degradation, methylation analysis, and nmr spectroscopy, including two-dimensional rotating-frame noe spectroscopy (roesy) and 1h,13c heteronuclear multiple-quantum coherence (hmqc) experiments. together with d-glucose and 2-acetamido-2-deoxy-d-glucose, the polysaccharide was found to contain two rarely occurring sugars, 6-deoxy-l-talose (l-6dtal) and 2,3-diacetam ...199910215848
cloning and sequence analysis of the lipase and lipase chaperone-encoding genes from acinetobacter calcoaceticus rag-1, and redefinition of a proteobacterial lipase family and an analogous lipase chaperone family.the genes encoding the lipase (lipa) and lipase chaperone (lipb) from acinetobacter calcoaceticus rag-1 were cloned and sequenced. the genes were isolated from a genomic dna library by complementation of a lipase-deficient transposon mutant of the same strain. transposon insertion in this mutant and three others was mapped to a single site in the chaperone gene. the deduced amino acid (aa) sequences for the lipase and its chaperone were found to encode mature proteins of 313 aa (32.5kda) and 347 ...199910216267
macromolecular antimicrobial glycoprotein, achacin, expressed in a methylotrophic yeast pichia pastoris.a cdna encoding achacin, an antimicrobial glycoprotein from the body surface mucus of giant african snail achacina fulica férussac, was expressed in a methylotrophic yeast, pichia pastoris, and recombinant achacin (rach) was secreted in yeast minimal medium in a polyglycosylated form with 80 kda. carbohydrate analysis revealed that the glycosylated moiety of rach was composed of 50 mol mannose and 2 mol n-acetylglucosamine residues. antimicrobial activity using escherichia coli and staphylococcu ...199910217406
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1997). i. susceptibility distribution].the frequencies of isolation and susceptibilities to antimicrobial agents were investigated on 560 bacterial strains isolated from patients with urinary tract infections (utis) in 9 hospitals during the period of june 1997 to may 1998. of the above bacterial isolates, gram-positive bacteria accounted for 29.3% and a majority of them were enterococcus faecalis. gram-negative bacteria accounted for 70.7% and most of them were escherichia coli. susceptibilities of several isolated bacteria to antim ...199910221177
in vitro evaluation of g1: a novel antimicrobial compound.g1 (1-[5-bromofur-2-il]-2-bromo-2-nitroethene) is a novel antimicrobial compound developed in cuba with reported broadspectrum activity against gram-positive and -negative bacteria, yeasts and fungi. a compound of this nature may have considerable therapeutic potential. we tested the in vitro activity of this novel compound against 3595 organisms using microbroth dilution. the following are mic50, mic90 and range respectively for some of the microorganisms tested: e. coli 16, 16, 4 32; klebsiell ...199910221421
arteriovenous and lymphatic malformations, linear verrucous epidermal nevus and mild overgrowth: another hamartoneoplastic syndrome?we report a 22 year old female presenting with slowly progressive paraparesis, who appeared to have many (mainly subcutaneous) hamartomas. the neurological symptoms were caused by intraspinal masses and arteriovenous malformations. in addition, she had mild overgrowth of one leg and lymph vessel malformations. this combination of symptoms resembles proteus syndrome, but is different in symptomatology and progression and may be yet another hamartoneoplastic syndrome.199910319199
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