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in vitro antimicrobial efficacy of a silver alginate dressing on burn wound isolates.to test the antimicrobial effectiveness of a silver alginate dressing on opportunistic pathogens, namely meticillin-sensitive staphylococcus aureus (mssa) and meticillin-resistant staphylococcus aureus (mrsa), klebsiella spp., enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, escherichia coli, enterobacter sakazakii, enterobacter cloacae, serratia marcescens, chryseobacterium indologenes, proteus vulgaris and acinetobacter baumannii.201121537295
synthesis and antimicrobial activities of hexahydroimidazo[1,5-a]pyridinium bromides with varying benzyl substituents.variously substituted benzyl bromides were employed to quaternize hexahydrobenzylimidazo[1,5-a]pyridine (a) and the resulting bromides (1-11) were evaluated for their in vitro antimicrobial activity against 10 pathogenic microorganisms: staphylococcus aureus, staphylococcus epidermidis, bacillus cereus, micrococcus luteus, proteus vulgaris, escherichia coli, salmonella typhimurium, klebsiella pneumonia, candida albicans and candida krusei. antimicrobial activities were surprisingly high (mic: 0. ...201121543140
pulmonary manifestations in proteus syndrome: pulmonary varicosities and bullous lung disease.we report on two patients with proteus syndrome (ps), with emphasis on its pulmonary manifestations. the first patient was a 6-year-old girl diagnosed with ps at 5 years of age. the pulmonary abnormalities first observed at age 3 years and included streaky densities with accentuated vascular markings detected by chest radiography. the patient had persistent abnormalities on follow-up chest radiographs. chest computed tomography (ct) scans showed diffuse pulmonary venous dilatations. the second p ...201121412980
cold atmospheric plasma: a successful treatment of lesions in hailey-hailey disease. 201121482888
potentiation of antimicrobial activity of ciprofloxacin by pelargonium graveolens essential oil against selected uropathogens.the recent approach of using herbs and antibiotics in combination constitutes a strategy to overcome the problems of resistance and side effects associated with conventional antibiotics. in the present study, the antimicrobial effect of pelargonium graveolens l' hér essential oil in combination with ciprofloxacin was evaluated on uropathogens, namely, klebsiella pneumoniae kt2, proteus mirabilis prt3 and staphylococcus aureus st2. minimum inhibitory concentrations of p. graveolens essential oil ...201121618302
phytochemical screening and antibacterial activities of vernonia ambigua, vernonia blumeoides and vernonia oocephala (asteraceae).some vernonia species (vernonia ambigua, vernonia blumeoides and vernonia oocephala) used in northern nigerian traditional medicine, were subjected to phytochemical screening using standard procedures. the antibacterial activity using the disc diffusion method as outlined by the nccls was carried out on klebsiella pneumoniae, streptococcus pyogenes, staphylococcus aureus, corynbacterium ulcerans, methicillin resistant staphylococcus aureus (mrsa), salmonella typhi, pseudomonas aeruginosa, shigel ...201121485703
[cmy-2-type plasmid-mediated ampc ß-lactamases emerging in tucumán, argentina].in the last years, enterobacteriaceae such as klebsiella pneumoniae, proteus mirabilis and escherichia coli, have acquired resistance to third-generation cephalosporins (c3g) because of the presence of plasmid-mediated ampc ß-lactamases. the aim of this work was to detect plasmid ampc enzymes and to investigate the predominant types in our region. between march and july 2009, 733 consecutive isolates of enterobacteriaceae derived from hospitals and outpatient centers were studied. susceptibility ...201121491062
superior in vitro activity of ertapenem and piperacillin/tazobactam against recent clinical isolates of proteus mirabilis from intra-abdominal infections (smart 2009-2010). 201121632217
antibacterial activity of selected ethnomedicinal plants from south india.to screen the antimicrobial potential of three ethnomedicinal plants chassalia curviflora thw. (c. curviflora), cyclea peltata hook. f. & thomson (c. peltata) and euphorbia hirta l (e. hirta) used in folk medicines in aarukani hills kani tribe, tamil nadu, india against human bacterial pathogens.201121771680
antibacterial activity of leaves and inter-nodal callus extracts of mentha arvensis l.to determine the anti-bacterial efficacy of chloroform, ethanol, ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from mentha arvensis (m. arvensis) against salmonella typhi(s. typhi), streptococcus pyogenes(s. pyogenes), proteus vulgaris(p. vulgaris) and bacillus subtilis(b. subtilis).201121771452
setting interpretive breakpoints for antimicrobial susceptibility testing using disk diffusion.antimicrobial susceptibility testing plays a key role in clinical microbiology. the disk diffusion test dates back to the 1940s and became standardised from the 1950s, with the international collaborative study (ics) and national committee for clinical laboratory standards (nccls) as the two major standards. interlaboratory variation of disk test results was recognised early but has never been dealt with in a satisfactory manner. the error-rate bounded method was described in 1974 and its role i ...201121640560
recognition of dna by the helix-turn-helix global regulatory protein lrp is modulated by the amino terminus.the asnc/lrp family of regulatory proteins links bacterial and archaeal transcription patterns to metabolism. in escherichia coli lrp regulates approximately 400 genes, over 200 of them directly. in earlier studies, lrp genes from vibrio cholerae, proteus mirabilis, and e. coli were introduced into the same e. coli background, and yielded overlapping but significantly different regulons. these differences were seen despite amino acid sequence identities of 92% (vibrio) and 98% (proteus) to e. co ...201121642464
susceptibility of esbl-producing enterobacteriaceae with the new clsi breakpoints.in 2010 clsi lowered the susceptibility breakpoints of some cephalosporins and aztreonam for enterobacteriaceae and eliminated the need to perform esbl screening and confirmatory tests. the aim of this study was to determine how many esbl-producing strains will test susceptible for three common species of enterobacteriaceae using the new breakpoints. as determined with the clsi screening and confirmatory tests, 382 consecutive esbl-producing strains were collected at our hospital between 2007 an ...201121752977
production of bioactive sheep β-defensin-1 in pichia pastoris.previous research has shown that sheep β-defensin-1 (sbd-1), a small cationic peptide with a broad range of antimicrobial activities, could inhibit the growth of both gram-positive and gram-negative bacteria as well as that of fungi. in order to increase the yield of current ovine defensin purification methods, mature sbd-1 (msbd-1) was added with a 6-his tag on the c-terminus (msbd-1-t) and expressed in pichia pastoris in the presented work. the msbd-1 and msbd-1-t were expressed in the pichia ...201121643707
microbiology at first visit of moderate-to-severe diabetic foot infection with antimicrobial activity and a survey of quinolone monotherapy.samples from 1295 patients with diabetic foot infection were evaluated; 4332 samples were collected with an average of 3.3 samples per patient. fifty-seven percent of patients had a 2b ulcer and 23% had a 3b ulcer according to texas university classification. in 64.2% of samples collected at first visit an etiologic agent was identified. about 40% of the positive samples were polymicrobial. gram positive bacteria were more frequently isolated (52.6%), staphylococcus aureus was the most frequentl ...201121824673
synthesis of p-triazinylphosphonium salts--hybrid molecules with potential antimicrobial activity.the new hybrid drugs combining in a single molecule triazine ring attached to phosphonium salt were prepared and their bactericidal activity against gram-positive bacteria staphylococcus aureus atcc43300 (mrsa - methycyline resistant staphylococcus aureus strain) two gram-negative bacteria escherichia coli ccug31997 serotype o153 (epec - enteropathogenic escherichia coli strain), proteus mirabilis 1784 (mdr - multidrug resistant clinical strain) were determined using microdilution method.201121648193
metallo-β-lactamases among enterobacteriaceae from routine samples in an italian tertiary-care hospital and long-term care facilities during 2008.the emergence of metallo-β-lactamase (mbl)-producing enterobacteriaceae is a serious public health concern. producers have been repeatedly isolated from patients and long-term care facility (ltcf) residents around bolzano, and we sought to assess their prevalence and clinical impact. all routine enterobacteriaceae isolates from a bolzano tertiary-care hospital and associated long-term care facilities in 2008 (n = 5500) were screened for mbls, with case details reviewed for the source patients. i ...201120345467
structure and serology of o-antigens as the basis for classification of proteus strains.this review is devoted to structural and serological characteristics of the o-antigens (o-polysaccharides) of the lipopolysaccharides of various proteus species, which provide the basis for classifying proteus strains to o-serogroups. the antigenic relationships of proteus strains within and beyond the genus as well as their o-antigen-related bioactivities are also discussed.201120305038
cadmium chloride exhibits a profound toxic effect on bacterial microflora of the mice gastrointestinal tract.cadmium (cd²(+)), a naturally occurring heavy metal, is an important environmental pollutant and a potent toxicant to bacteria. the gastrointestinal (gi) tract microflora has a marked capacity to cope with the increased load of ingested metals. however, heavy metals may have harmful effects on git microflora. under the conditions of experimental exposure to cadmium, changes in the population of intestinal microflora in healthy mice were examined. five experimental groups received 23 to 50 mg kg⁻ ...201120488844
antimicrobial susceptibility of pediatric uropathogens in thrace, greece.the aim of this study was to investigate the bacterial pathogens involved in pediatric urinary tract infections (utis) in a tertiary general hospital located in the thrace province of northern greece over a 69-month period (1/2003 to 9/2008), and their antibiotic susceptibility patterns. a total of 622 episodes of utis in 508 children were identified. median age of all children was 16 months (range 1 month to 14 years). boys were significantly younger than girls (9 months vs. 24 months). escheri ...201120524067
antibacterial activity of extracts from plants of central argentina--isolation of an active principle from achyrocline satureioides.the great increase in bacterial infections is fueling interest in the search for antibacterial products of plant origin. extracts obtained from 51 native and naturalized plants from central argentina were therefore evaluated for their in vitro inhibitory activity on pathogenic bacteria with the aim of selecting the most active ones as new sources of effective antibiotics. the susceptibility of reference and clinical strains of enterococcus faecalis, escherichia coli, proteus mirabilis, pseudomon ...201120645245
aerobic bacteria from mucous membranes, ear canals, and skin wounds of feral cats in grenada, and the antimicrobial drug susceptibility of major isolates.in a 2-year period 54 feral cats were captured in grenada, west indies, and a total of 383 samples consisting of swabs from rectum, vagina, ears, eyes, mouth, nose and wounds/abscesses, were cultured for aerobic bacteria and campylobacters. a total of 251 bacterial isolates were obtained, of which 205 were identified to species level and 46 to genus level. a commercial bacterial identification system (api/biomerieux), was used for this purpose. the most common species was escherichia coli (n=60) ...201120627391
extreme lifespan of the human fish (proteus anguinus): a challenge for ageing mechanisms.theories of extreme lifespan evolution in vertebrates commonly implicate large size and predator-free environments together with physiological characteristics like low metabolism and high protection against oxidative damages. here, we show that the 'human fish' (olm, proteus anguinus), a small cave salamander (weighing 15-20 g), has evolved an extreme life-history strategy with a predicted maximum lifespan of over 100 years, an adult average lifespan of 68.5 years, an age at sexual maturity of 1 ...201120659920
electrical microcurrent to prevent conditioning film and bacterial adhesion to urological stents.long-term catheters remain a significant clinical problem in urology due to the high rate of bacterial colonization, infection, and encrustation. minutes after insertion of a catheter, depositions of host urinary components onto the catheter surface form a conditioning film actively supporting the bacterial adhesion process. we investigated the possibility of reducing or avoiding the buildup of these naturally forming conditioning films and of preventing bacterial adhesion by applying different ...201120686759
[etiology and sensitivity of uropathogens identified in uncomplicated lower urinary tract infections in women (aresc study): implications on empiric therapy].to determine the etiology and susceptibility of uropathogens identified in women with uncomplicated lower urinary tract infections (uti).201120889171
cajanol inhibits the growth of escherichia coli and staphylococcus aureus by acting on membrane and dna damage.in the present study, the mechanism of antibacterial activity of cajanol extracted from the roots of cajanus cajan (l.) millsp. towards escherichia coli (e. coli) and staphylococcus aureus (s. aureus) was investigated. the antibacterial activity of cajanol was evaluated towards six bacterial strains (staphylococcus epidermidis, staphylococcus aureus, bacillus subtilis, escherichia coli, proteus vulgaris, and pseudomonas aeruginosa) by the broth microdilution method. it showed strong antibacteria ...201120803417
effect of uropathogens on in vitro encrustation of polyurethane double j ureteral stents.polyurethane stents are used when there is an obstruction to the flow of urine. a majority of the patients with such stents are at the risk of urinary tract infection and salt encrustation. the present study is aimed at analyzing the in vitro encrustation of calcium oxalate and other salts in the presence of common uropathogens (e. coli and p. mirabilis) on films made from tecoflex(®), a commercial grade polyurethane. in the absence of uropathogens, sodium ions and ammonia favor calcium adsorpti ...201120517602
clinical and molecular epidemiology of extended-spectrum β-lactamase-producing escherichia coli and klebsiella pneumoniae in a long-term study from japan.the detection rates of extended-spectrum β-lactamase (esbl)-producing bacteria in japan are very low (∼5%) compared with those obtained worldwide. further, the current trend of these bacteria in japan is not known, and few studies with longitudinal observations have been reported. to obtain epidemiologic data on esbl-producing bacteria, their genotypic features, and their antibiotic resistance patterns in japan, we analyzed bacterial isolates from hospitalized patients at our institution over th ...201120859753
antimicrobial activity of erythromycin and clarithromycin against clinical isolates of escherichia coli, staphylococcus aureus, klebsiella and proteus by disc diffusion method.fifty clinical isolates comprising of escherichia coli, staphylococcus aureus, klebsiella and proteus were collected from different local pathological laboratories and their resistant pattern against two well known macrolides; erythromycin and clarithromycin were studied using disc diffusion method. klebsiella (41.67% against erythromycin and 58.34% against clarithromycin) and proteus (66.67% against erythromycin and clarithromycin) species were found to be more resistant against the studied mac ...201121190914
efficient secretion of biologically active chondroitinase abc from mammalian cells in the absence of an n-terminal signal peptide.proteoglycans carrying chondroitin sulfate side chains have been shown to fulfill important biological functions in development, disease, and signaling. one area of considerable interest is the functional importance of chondroitin sulfates as inhibitors of the regeneration of axonal projections in the mammalian central nervous system. in animal models of spinal cord injury, injections of the enzyme chondroitinase abc from the bacterium proteus vulgaris into the lesion site leads to degradation o ...201121213020
risk factors for hospital-acquired urinary tract infection: a case-control study.the objective is to assess risk factors and microbiological aspects of hospital-acquired urinary tract infection (hauti) on six wards of a general regional hospital in serbia. a case-control study was nested within prospective cohort hautis study conducted from january 1 to december 31, 2007. three controls were identified for each patient with hauti, being chronologically the next three patients surveyed who did not develop hauti. the patients and controls were matched by sex and age (±5 years) ...201120848199
influence of referral bias on the clinical characteristics of patients with gram-negative bloodstream infection.summaryreferral bias can influence the results of studies performed at tertiary-care centres. in this study, we evaluated demographic and microbiological factors that influenced referral of patients with gram-negative bloodstream infection (bsi). we identified 2919 and 846 unique patients with gram-negative bsi in a referral cohort of patients treated at mayo clinic hospitals and a population-based cohort of olmsted county, minnesota, residents between 1 january 1998 and 31 december 2007, respec ...201121281552
suprathel-antiseptic matrix: in vitro model for local antiseptic treatment?acetic acid is a traditional antiseptic agent that has been used for more than 6000 years. the main goal of this study was to demonstrate the suitability of suprathel (polymedics innovations gmbh, denkendorf, germany) in combination with various antiseptic agents to create an "antiseptic-matrix" especially designed for problematic microorganisms such as proteus vulgaris, acinetobacter baumannii, or pseudomonas aeruginosa, which are frequently associated with burns. the study was designed to test ...201121242734
thrombocytes are effectors of the innate immune system releasing human beta defensin-3.thrombocyte concentrate i.e. platelet-rich plasma (prp) has become a popular adjunct for many surgical procedures. it is believed to improve bone and soft tissue healing. recently antimicrobial effects of the autologous preparation were reported by several groups. in this study we investigated the antimicrobial effect of prp against gram-negative microbes which frequently cause severe complications in orthopaedic trauma surgery.201121310405
production of biogenic amines by lactic acid bacteria and enterobacteria isolated from fresh pork sausages packaged in different atmospheres and kept under refrigeration.the occurrence of in vitro amino acid activity in bacterial strains associated with fresh pork sausages packaged in different atmospheres and kept in refrigeration was studied. the presence of biogenic amines in decarboxylase broth was confirmed by ion-exchange chromatography and by the presence of the corresponding decarboxylase genes by pcr. from the 93 lactic acid bacteria and 100 enterobacteria strains analysed, the decarboxylase medium underestimates the number of biogenic amine-producer st ...201121316866
[nosocomial sinusitis in an intensive care unit: a microbiological study].nosocomial sinusitis is a common complication of patients in icus. its diagnosis is important, and early treatment is required to avoid serious complications such as pneumonia, sepsis, meningitis, and intracranial abscesses.201121340197
carijodienone from the octocoral carijoa multiflora. a spiropregnane-based steroid.two new steroids (1 and 2) and the known pregna-1,4,20-trien-3-one (3) have been isolated from the pacific octocoral carijoa multiflora. compound 1 possesses a novel spiropregnane-based steroidal skeleton. the photochemical transformation of 3 into 1 allowed the assignment of the absolute configuration at c-10 of 1. the antibacterial activities of compounds 1 and 3 were evaluated against a panel of bacterial strains.201121235217
in vitro activity of ceftobiprole against frequently encountered aerobic and facultative gram-positive and gram-negative bacterial pathogens: results of the canward 2007-2009 study.the in vitro activity of ceftobiprole was evaluated against 15 011 clinical isolates obtained from patients in canadian hospitals between 2007 and 2009. all staphylococcus aureus were susceptible to ceftobiprole (mic(90)'s for methicillin-susceptible staphylococcus aureus and methicillin-resistant staphylococcus aureus of = 1 µg/ml and 2 µg/ml, respectively). ceftobiprole was active against penicillin-susceptible streptococcus pneumoniae (mic(90), = 0.06 µg/ml), penicillin-resistant streptococcu ...201121353964
idiopathic corporeal hemihypertrophy associated with hemihypertrichosis.the hemihypertrophy or hemihyperplasy is a rare congenital abnormality, characterized by an asymmetric growth of the limbs, the trunk, and the face or half of the entire body. it may be isolated or be part of several syndromes including beckwith-wiedemann syndrome, klippel-trenaunay-weber syndrome, silver-russell syndrome and proteus syndrome. in its isolated form, it is called idiopathic. the latter may be associated with several anomalies including dermatological and urogenital abnormalities w ...201121232734
in vitro activity of ceftaroline against gram-positive and gram-negative pathogens isolated from patients in canadian hospitals in 2009.the in vitro activities of ceftaroline and comparative agents were determined for a collection of the most frequently isolated bacterial pathogens from hospital-associated patients across canada in 2009 as part of the ongoing canward surveillance study. in total, 4,546 isolates from 15 sentinel canadian hospital laboratories were tested using the clinical and laboratory standards institute (clsi) broth microdilution method. compared with other cephalosporins, including ceftobiprole, cefepime, an ...201121402844
treatment of endocarditis due to proteus species: a literature review.endocarditis due to proteus species is very rare. we report a case of endocarditis due to proteus mirabilis that was successfully treated with ampicillin and gentamicin, and review the treatment regimens of previously published cases of proteus endocarditis.201121237686
enterobacterial microflora in infancy - a case study with enhanced enrichment.to validate pmeu (portable microbe enrichment unit) method for monitoring the composition and development of infantile intestinal enterobacterial microflora.201121249478
chromosome-encoded ampc and ctx-m extended-spectrum ß-lactamases in clinical isolates of proteus mirabilis from korea.among 222 proteus mirabilis clinical isolates collected from 17 hospitals in korea in 2008, 28 (12.6%) and 8 (3.6%) isolates exhibited extended-spectrum ß-lactamase (esbl) and ampc phenotypes, respectively. the most common type of esbl gene identified by pcr and sequencing experiments was bla(ctx-m-14a) (n = 12). the bla(ctx-m-90) (n = 4), bla(ctx-m-15) (n = 3), bla(ctx-m-12) (n = 3), bla(ctx-m-2) (n = 2), bla(ctx-m-14b) (n = 1), bla(tem-52) (n = 5), and bla(shv-12) (n = 1) genes were also detec ...201121282448
the free-radical damage theory: accumulating evidence against a simple link of oxidative stress to ageing and lifespan.recent work on a small european cave salamander (proteus anguinus) has revealed that it has exceptional longevity, yet it appears to have unexceptional defences against oxidative damage. this paper comes at the end of a string of other studies that are calling into question the free-radical damage theory of ageing. this theory rose to prominence in the 1990s as the dominant theory for why we age and die. despite substantial correlative evidence to support it, studies in the last five years have ...201121290398
nasal immunization with attenuated salmonella typhimurium expressing an mrpa-tetc fusion protein significantly reduces proteus mirabilis colonization in the mouse urinary tract.the development of effective strategies to prevent urinary tract infections (utis) has become an important goal in public health. proteus mirabilis is commonly associated with complicated utis and expresses several virulence factors, including mannose-resistant proteus-like (mr/p) fimbriae. here, a fusion protein formed from mrpa, the structural protein of mr/p fimbriae, and tetc, a non-toxic but highly immunogenic fragment of tetanus toxin, to be delivered by an attenuated salmonella typhimuriu ...201121415205
expression and characterization of a second l-amino acid deaminase isolated from proteus mirabilis in escherichia coli.l-amino acid deaminases catalyze the deamination of natural l-amino acids. two types of l-amino acid deaminase have been identified in proteus species. one exhibits high levels of activity toward a wide range of aliphatic and aromatic l-amino acids, typically l-phenylalanine, whereas the other acts on a relatively narrow range of basic l-amino acids, typically l-histidine. in this study, we cloned, expressed, and characterized a second amino acid deaminase, termed pm1, from p. mirabilis kctc 256 ...201121298676
antibacterial activity of the indigenous earthworms lampito mauritii (kinberg) and perionyx excavatus (perrier).earthworms respond to microbial infection through cellular and humoral defense mechanisms such as antimicrobial protein secretions. most of the humoral defense proteins are synthesized in the skin itself.201121299431
monitoring of multiple drug-resistant pathogens in a selected stretch of bay of bengal, india.the present work aims at identification of multiple drug-resistant pathogenic bacteria in a selected stretch, namely, puri on the bay of bengal, india. six stations at the coast of puri were selected and samples of water and sediment were collected during the winter of 2008 and 2009 for this study. thirty-eight pathogenic bacteria were isolated and identified from both the water and the sediment of 6 fixed stations (pu-1a, pu-1b, pu-2, pu-3, pu-4, and pu-5). the identified pathogens were escheri ...201121424669
neurofibromatosis type 1: persisting misidentification of the "elephant man" disease.during informal interviews in the course of an ethnographic study on intergenerational dialogue between individuals with neurofibromatosis and their parents, many members of canadian neurofibromatosis associations have stated that they continue to be told the condition that afflicts them or their children is "elephant man's disease." today, even though well-established clinical criteria make it possible to diagnose and differentiate the 2 diseases, the confusion between neurofibromatosis type 1 ...201121209351
volatile compounds and antimicrobial and antioxidant activities of the essential oils of the needles of pinus densiflora and pinus thunbergii.to investigate the volatile compounds and the antibacterial and antioxidant effects of the essential oils of pinus densiflora needles (epdn) and pinus thunbergii needles (eptn), the volatile compounds of steam-distilled essential oils were analysed by gas chromatography-mass spectrometry. antibacterial activities were analysed by performing disc-agar diffusion assay and determining the minimum inhibitory concentrations (mics) of the essential oils. antioxidant activities were analysed via radica ...201121213230
cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids.some natural products consisting of the alkaloids yohimbine and vincamine (indole-type), scopolamine and atropine (tropane-type), colchicine (tropolone-type), allantoin (imidazolidine-type), trigonelline (pyridine-type) as well as octopamine, synephrine, and capsaicin (exocyclic amine-type); the flavonoid derivatives quercetin, apigenin, genistein, naringin, silymarin, and silibinin; and the phenolic acids namely gallic acid, caffeic acid, chlorogenic acid, and quinic acid, were tested for their ...201121391841
clinical, immunological and genetic features in taiwanese patients with the phenotype of hyper-immunoglobulin e recurrent infection syndromes (hies).hyper-immunoglobulin e recurrent infection syndromes (hies) have distinct features, with identified associated mutations of stat3, tyk2, and dock8. among 197 taiwanese patients with primary immunodeficiency on a referral-base of over 23 million inhabitants, stat3 (r382w and q469r) and dock8 mutations (exon 1-9 deletion) were identified in two patients each from six ad-hies and five ar-hies patients, respectively. aside from decreased th17 and memory b cells, characteristic facies and pneumatocel ...201121324546
characterization of the enterobacteriaceae community that developed during storage of minced beef under aerobic or modified atmosphere packaging conditions.the whole cell protein and macrorestriction analysis of dna of enterobacteriaceae isolates recovered from minced beef stored at 0, 5, 10 and 15 °c aerobically and under modified atmosphere packaging consisting of 40% co(2)-30% o(2)-30% n(2) in the presence (map+) and absence (map-) of oregano essential oil were studied. sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) profiles obtained from whole cell protein analysis of the enterobacteriaceae isolates revealed seven groups. ...201021216482
the prevalence and bacteriology of asymptomatic bacteriuria among antenatal patients in nnamdi azikiwe university teaching hospital nnewi; south eastern nigeria.background: urinary tract infection in pregnancy leads to poor pregnancy outcome. diagnosis and treatment of asymptomatic bacteriuria markedly improves pregnancy outcome as well as reduce the incidence of acute pyelonephritis. objective: to determine the prevalence and bacteriology of asymptomatic bacteriuria among antenatal patients in our centre, and to know if routine screening will be justifiable. materials and methods: this was a prospective study carried out between april and august 2008. ...201021220855
[immunovac-vp-4 for prevention of acute respiratory diseases in children's communities].assessment of prophylactic effect of immunovac-vp-4 against acute respiratory diseases (ards) in collectives of children.201021381375
antimicrobial activity of α-(2-hydroxy-2-methylpropyl)-ω-(2-hydroxy-3-methylbut-2-en-1-yl) polymethylene from caesalpinia bonducella (l.) flem.the compound, α-(2-hydroxy-2-methylpropyl)-ω-(2-hydroxy-3-methylbut-2-en-1-yl)polymethylene, isolated from ethyl acetate leaf extract of caesalpinia bonducella (l.) flem. was evaluated for antimicrobial activity against clinical isolates, proteus vulgaris, pseudomonas aeruginosa, klebsiella sp., staphylococcus citrus, staphylococcus aureus, escherichia coli, candida albicans and rhodotorula sp. using agar diffusion method. the compound exerted inhibitory zone at all concentrations and revealed t ...201021218063
[antimicrobial susceptibility of clinical isolates of aerobic gram-negative bacteria in 2006].we determined mics of antibacterial agents against 1280 clinical strains of aerobic gram-negative bacteria (19 genus or species) isolated at 16 japanese facilities in 2006. mics were determined using mostly broth microdilution method and antibacterial activity was assessed. strains producing extended-spectrum beta-lactamases (esbl) accounted for 3.7% of escherichia coli, 2.7% of klebsiella spp., and 11.4% of proteus spp. notably, 18.8% of proteus mirabilis was found to produce esbl higher than 1 ...201021425597
thyroid pathology in pten-hamartoma tumor syndrome: characteristic findings of a distinct entity.phosphatase and tensin homolog deleted on chromosome ten (pten)-hamartoma tumor syndrome (phts) is a complex disorder caused by germline inactivating mutations of the pten tumor suppressor gene. phts includes cowden syndrome (cs), bannayan-riley-ruvalcaba syndrome (brrs), and proteus-like syndromes. affected individuals develop both benign and malignant tumors in a variety of tissues, including the thyroid. this study is to better characterize and describe the thyroid pathology within the differ ...201021190448
[epidemiological and bacteriological characteristics of uropathogen bacteria isolated in a pediatric environment].urinary tract infection (uti) in children is a grave pathology, which requires a fast and effective care. bacteriological and epidemiological data play a determining role in patient's care. we report a retrospective study, which spreads out from january 1st till august 31st, 2009, having concerned hospitalized children for urinary infection in pediatrics service of farhat hached teaching hospital in sousse. our series contained 51 children with a sex ratio of 0.76, an average age of 32 months. t ...201021111582
synthesis and antimicrobial activity of highly functionalised novel β-lactam grafted spiropyrrolidines and pyrrolizidines.a facile and one-pot synthesis of a series of novel spiropyrrolidines/pyrrolizidines with β-lactam substituent has been accomplished through 1,3-dipolar cycloaddition reaction of alkenyl esters derived from β-lactam aldehyde as dipolarophile with the dipole azomethine ylide derived from 1,2- and 1,3-diketones and secondary amino acids. the synthesized compounds were evaluated for antimicrobial activities and found to exhibit relatively good antibacterial activity at lower concentration against f ...201021177001
asymptomatic bacteriuria among hiv positive pregnant women.the prognostic significance of asymptomatic bacteriuria resides in the observation that the prevalence is, relatively, high in persons with certain medical conditions, such as diabetes mellitus and pregnancy. this prevalence might, even, be higher in patients with human immunodeficiency virus infection. hence, this study set out to determine the prevalence of asymptomatic bacteriuria among symptom free and newly enrolled hiv infected pregnant women attending pmtct unit of antiretroviral clinic o ...201021178431
effect of neem extract against the bacteria isolated from marine fish.marine ornamental fishes are exceedingly valuable due to their high demand in domestic and international markets. there is a growing global interest to rear the fishes in captivity. but problem due to bacteria and fungi are the major hitch in captive condition. since, the use of antibiotics is banned, an attempt was made to ascertain in vitro assay of the neem leaves extract against the bacterial pathogens isolated from infected fishes. bacterial strains isolated from infected regions of the clo ...201021186711
the salmonella genomic island 1 is specifically mobilized in trans by the inca/c multidrug resistance plasmid family.the salmonella genomic island 1 (sgi1) is a salmonella enterica-derived integrative mobilizable element (ime) containing various complex multiple resistance integrons identified in several s. enterica serovars and in proteus mirabilis. previous studies have shown that sgi1 transfers horizontally by in trans mobilization in the presence of the inca/c conjugative helper plasmid pr55.201021187963
photodynamic therapy with a cationic functionalized fullerene rescues mice from fatal wound infections.fullerenes are under intensive study for potential biomedical applications. we have previously reported that a c60 fullerene functionalized with three dimethylpyrrolidinium groups (bf6) is a highly active broad-spectrum antimicrobial photosensitizer in vitro when combined with white-light illumination. we asked whether this high degree of in vitro activity would translate into an in vivo therapeutic effect in two potentially lethal mouse models of infected wounds.201021143031
structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o60.an o-polysaccharide (o-antigen) was isolated by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o60 and studied by sugar and methylation analyses as well as (1)h and (13)c nmr spectroscopy, including 2d roesy and (1)h,(13)c hmbc experiments in d(2)o and a roesy experiment in a 9:1 h(2)o-d(2)o mixture to reveal correlations for nh protons. it was found that the polysaccharide is built up of linear pentasaccharide repeating units containing an amide of d-glucuronic aci ...201021146157
[comparison of four phenotypic methods to detect extended-spectrum betalactamases].to compare the efficacy of four phenotypic methods for the identification of strains producing extended-spectrum β-lactamases isolated from urine cultures.201021152726
unexpected coregulator range for the global regulator lrp of escherichia coli and proteus mirabilis.the lrp/asnc family of transcription factors links gene regulation to metabolism in bacteria and archaea. members of this family, collectively, respond to a wide range of amino acids as coregulators. in escherichia coli, lrp regulates over 200 genes directly and is well known to respond to leucine and, to a somewhat lesser extent, alanine. we focused on lrp from proteus mirabilis and e. coli, orthologs with 98% identity overall and identical helix-turn-helix motifs, for which a previous study ne ...201021169483
akara ogbomoso: microbiological examination and identification of hazards and critical control points.akara ogbomoso was examined toward the establishment of hazard analysis and critical control point (haccp). the akara was produced in residential buildings with the attendant consequence of contamination. there was ample growth of aerobes, coliforms, staphylococci, shigella and yeast/mold from the samples, water and cowpea pastes. microbial contaminations occur through the processing, which can be corrected through education by adopting good hygienic and manufacturing practices. the critical con ...201021339157
[occurrence of 16s rrna methylase arma producing enterobacteriaceae in a general hospital in warsaw, poland].resistance to gentamicin, amikacin and kanamycin was screened in 270 clinical isolates of enterobacteriaceae originated from april 19 to may 19, 2010 in a regular hospital in warsaw, poland. most of the isolated bacteria were considered pathogenic. nineteen isolates (7%) were simultaneously resistant to two or three of the tested aminoglycosides. mics of the three aminoglycosides ranged form 128 to 1024 mcg/ml for six isolates. these isolates were suspected to produce 16s rrna methylase. genes e ...201021114012
plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing enterobacteriaceae in morocco.although independently acquired, plasmid-mediated quinolone resistance appears to be linked with extended-spectrum or ampc-type beta-lactamases. since no data are available in african countries, the prevalence of qnr genes at the university hospital ibn rochd, casablanca, morocco, was investigated.201021252459
assessment of effectiveness and safety of ibicella lutea extract in the control of experimental proteus mirabilis urinary tract infection.proteus mirabilis is an important cause of complicated urinary tract infections (uti). like many other microorganisms, p. mirabilis has acquired resistance to many antibiotics. due to the serious effects associated with uropathogenic p. mirabilis and the problems related to the use of antibiotics, alternative strategies for its control must be developed. previously, we studied the effect of ibicella lutea extract, a south american indigenous plant, on in vitro uropathogenicity of p. mirabilis. w ...201021252462
predation of human pathogens by the predatory bacteria micavibrio aeruginosavorus and bdellovibrio bacteriovorus.the focus of this study was to evaluate the potential use of the predatory bacteria bdellovibrio bacteriovorus and micavibrio aeruginosavorus to control the pathogens associated with human infection.201021114596
clinical aspects and genetic analysis of taiwanese patients with the phenotype of hyper-immunoglobulin e recurrent infection syndromes (hies).hyper-immunoglobulin e recurrent infection syndromes (hies) has characteristic features and identified mutations. this study investigated clinical features and causal candidate mutations in taiwanese patients with the hies phenotype on referral base over 23 million inhabitants.201021120687
microbial evaluation and public health implications of urine as alternative therapy in clinical pediatric cases: health implication of urine therapy.cultural means of pediatric treatment during ill health is a mainstay in africa, and though urine has been known to contain enteric pathogens, urine therapy is still culturally applicable in some health conditions and also advocated as alternative therapy. the study therefore, is to evaluate the microbial contents and safety of urine.201021293739
laboratory detection of extended-spectrum-beta-lactamase-producing enterobacteriaceae: evaluation of two screening agar plates and two confirmation techniques.the worldwide prevalence of extended-spectrum-beta-lactamase-producing esbl-producing enterobacteriaceae (esbl-e) is increasing, making the need for optimized detection techniques more urgent. in this study we investigated the performance of two esbl-e screening and two esbl-e confirmation techniques. in accordance with the dutch national guidelines (www.wip.nl), a collection of 642 highly resistant enterobacteriaceae strains, as identified by vitek2, was used to test the performances of two scr ...201021123527
necrotising otitis externa: an unusual cause of cranial nerve palsy in a diabetic haemodialysis patient.we present an unusual case of necrotising otitis externa (noe) causing a lower motor neurone facial nerve palsy in a patient with diabetes mellitus and receiving maintenance haemodialysis for end-stage renal disease (esrd). pseudomonas aeruginosa is the most common pathogen isolated in noe, although our case involved the non-typical pathogens aspergillus flavus and proteus mirabilis. we discuss the need for diagnostic rigour and the importance of considering atypical infective pathology in patie ...201021125035
a child with mental retardation and asymmetrical hypertrophy of limbs.a 5-year-old male child presented with progressively increasing asymmetrical overgrowth of limbs, hyperpigmentation on the right half of the body, and mental retardation since 9 months of age.201021132570
prevalence and resistance patterns of extended-spectrum and ampc β-lactamase in escherichia coli, klebsiella pneumoniae, proteus mirabilis, and salmonella serovar stanley in a korean tertiary hospital.a total of 100 clinical isolates of escherichia coli (n = 35), klebsiella pneumoniae (n = 63), proteus mirabilis (n = 1), and salmonella serovar stanley (n = 1), showing resistance to cefoxitin, or returning positive in extended-spectrum β-lactamase (esbl) by clinical and laboratory standards institute (clsi) esbl confirmatory method, were studied. the isolates were examined by the boronic acid (ba) disk test, polymerase chain reaction, and pulsed-field gel electrophoresis (pfge) to investigate ...201020854475
prevalence of bacteria and parasites in white ibis in egypt.a field survey was conducted to evaluate the prevalence of bacterial infections among free-living white ibis (nipponia nippon) in which 92 bacterial isolates were recovered from 193 different internal organs of 55 apparently healthy ibis. escherichia coli and salmonella spp. were isolated at rates of 43.6% and 14.5%, respectively. the other bacterial pathogens isolated were shigella spp. (34.5%), enterobacter spp. (21.8%) citrobacter spp. (18.1%), klebsiella pneumonia (16.3), staphylococcus aure ...201020857378
c-terminal fragment of amebin promotes actin filament bundling, inhibits acto-myosin atpase activity and is essential for amoeba migration.amebin [formerly termed as apabp-fi; sobczak et al. (2007) biochem. cell biol. 85] is encoded in amoeba proteus by two transcripts, 2672-nt and 1125-nt. a product of the shorter transcript (termed as c-amebin), comprising c-terminal 375 amino-acid-residue fragment of amebin, has been expressed and purified as the recombinant gst-fusion protein. gst-c-amebin bound both to monomeric and filamentous actin. the binding was ca(2+)-independent and promoted filament bundling, as revealed with the trans ...201021094125
comparison of bacterial diversity in wheat bran and in the gut of larvae and newly emerged adult of musca domestica (diptera: muscidae) by use of ethidium monoazide reveals bacterial colonization.the objective of the current study is to investigate the bacterial colonization within the gut of the house fly, musca domestica l. (diptera: muscidae), at the larval stage and the bacterial community of the gut of the house fly at the newly emerged adult stage. after using ethidium monoazide to inhibit recovery of nucleic acids from dead bacteria, three polymerase chain reaction (pcr)-amplified 16s rdna libraries from wheat bran, larvae, and newly emerged adults was constructed, analyzed, and c ...201021061987
electron transfer from proteus vulgaris to a covalently assembled, single walled carbon nanotube electrode functionalised with osmium bipyridine complex: application to a whole cell biosensor.we report the fabrication and use of electrodes constructed from single walled carbon nanotubes (swcnts) chemically assembled on a carbon surface and functionalised with an osmium(ii) bipyridine complex (osbpy). the ability of the electrodes to transduce biologically generated currents from proteus vulgaris has been established. our investigations show that there are two contributions to the current: one from electroactive species secreted into solution and another from cell redox sites. the mod ...201021067910
synthesis and evaluation of a class of new coumarin triazole derivatives as potential antimicrobial agents.a series of new coumarin-based 1,2,4-triazole derivatives were designed, synthesized and evaluated for their antimicrobial activities in vitro against four gram-positive bacteria (staphylococcus aureus, mrsa, bacillus subtilis and micrococcus luteus), four gram-negative bacteria (escherichia coli, proteus vulgaris, salmonella typhi and shigella dysenteriae) as well as three fungi (candida albicans, saccharomyces cerevisiae and aspergillus fumigatus) by two-fold serial dilution technique. the bio ...201021215620
salmonella genomic islands and antibiotic resistance in salmonella enterica.antibiotic resistance in several salmonella enterica serovars that cause gastrointestinal disease in humans is due to a set of related genomic islands carrying a class 1 integron, which carries the resistance genes. salmonella genomic island 1 (sgi1), the first island of this type, was found in s. enterica serovar typhimurium dt104 isolates, which are resistant to ampicillin, chloramphenicol, florfenicol, streptomycin, spectinomycin, sulfonamides and tetracycline. several salmonella serovars and ...201021073312
high levels of multidrug resistance in clinical isolates of gram-negative pathogens from nigeria.in nigeria, quinolones and β-lactam antibiotics are widely used to treat bacterial infections. this study aimed to identify the prevalence of resistance to these drugs and to determine the mechanisms of resistance to these agents. in total, 134 non-duplicate, gram-negative enteric isolates of 13 species from different hospitals were investigated for susceptibility to a panel of antibiotics, carriage of plasmid-mediated quinolone and β-lactam resistance genes, production of extended-spectrum β-la ...201021074376
pan-susceptible proteus mirabilis septicemia in a patient multicolonized by pan-resistant bacteria. 201021193275
16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull.marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ...201021038149
characterization of epitope specificity of proteus penneri 7 lipopolysaccharide core region.to extend the knowledge on the fragments of proteus penneri lipopolysaccharide core regions, which determine the cross-reactions with specific antibodies, serological studies were performed by use of p. penneri 7 core-specific antiserum and proteus sp. lipopolysaccharides. different reactivity of the tested antiserum with three groups of antigens suggested differences in their core regions' epitope specificity. comparing the results of the serological investigations with the previously determine ...201021060898
[antibacterial antibodies in human immunoglobulins and sera: past and present].to measure levels of several types of antibacterial antibodies in preparations of normal human immunoglobulin as well as in samples of donor sera obtained in 1965 and 2009.201021061576
proteobactin and a yersiniabactin-related siderophore mediate iron acquisition in proteus mirabilis.proteus mirabilis causes complicated urinary tract infections (utis). while the urinary tract is an iron-limiting environment, iron acquisition remains poorly characterized for this uropathogen. microarray analysis of p. mirabilis hi4320 cultured under iron limitation identified 45 significantly upregulated genes (p ≤ 0.05) that represent 21 putative iron-regulated systems. two gene clusters, pmi0229-0239 and pmi2596-2605, encode putative siderophore systems. pmi0229-0239 encodes a non-ribosomal ...201020923418
three enzymatic steps required for the galactosamine incorporation into core lipopolysaccharide.the core lipopolysaccharides (lps) of proteus mirabilis as well as those of klebsiella pneumoniae and serratia marcescens are characterized by the presence of a hexosamine-galacturonic acid disaccharide (αhexn-(1,4)-αgala) attached by an α1,3 linkage to l-glycero-d-manno-heptopyranose ii (l-glycero-α-d-manno-heptosepyranose ii). in k. pneumoniae, s. marcescens, and some p. mirabilis strains, hexn is d-glucosamine, whereas in other p. mirabilis strains, it corresponds to d-galactosamine. previous ...201020959463
territoriality in proteus: advertisement and aggression. 201021162556
structure and gene cluster of the o-antigen of escherichia coli o109; chemical and genetic evidences of the presence of l-rhan3n derivatives in the o-antigens of e. coli o109 and o119.o-antigen representing the o-polysaccharide chain of the lipopolysaccharide is the most variable constituent on the cell surface of gram-negative bacteria and a player in their pathogenicity. the o-polysaccharide of escherichia coli o109 was studied by sugar analysis and nuclear magnetic resonance spectroscopy and found to contain a rarely occurring monosaccharide, 2,3-diacetamido-2,3,6-trideoxy-l-mannose (l-rhanac3nac). the following structure of the tetrasaccharide repeating unit of the o-poly ...201020964722
the nationwide study of bacterial pathogens associated with urinary tract infections conducted by the japanese society of chemotherapy.this study was conducted by the japanese society of chemotherapy and is the first nationwide study on bacterial pathogens isolated from patients with urinary tract infections at 28 hospitals throughout japan between january 2008 and june 2008. a total of 688 bacterial strains were isolated from adult patients with urinary tract infections. the strains investigated in this study are as follows: enterococcus faecalis (n = 140), escherichia coli (n = 255), klebsiella pneumoniae (n = 93), proteus mi ...201021174142
endovascular treatment of disseminated complex aortic vascular malformations in a patient with proteus syndrome. 201020971228
an illustrative case of neurofibromatosis type 1 and nf1 microdeletion.we report on a patient with nf1 microdeletion and clinical manifestations that fulfill the diagnostic criteria for neurofibromatosis type 1 but also presenting features reminiscent of proteus syndrome.201021031083
incidence of postoperative eye infections in a private eye hospital in lagos, nigeria.there is a dearth of information on postoperative eye infections in nigeria and most hospitals do not have an infection control program in place.201021033323
occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions.the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ...201021033566
emergence of klebsiella pneumoniae carbapenemase-producing proteus mirabilis in hangzhou, china.carbapenems are used to treat severe infections caused by multi-drug-resistant organisms, however, the emergence of carbapenem-resistant bacterial isolates is becoming an increasing therapeutic challenge. since the first klebsiella (k.) pneumoniae carbapenemase (kpc)-producing k. pneumoniae was reported in 2001, kpc-producing isolates have been found increasingly, specially in enterobacteriaceae. the aim of this study was to characterize the mechanisms of a carbapenem-resistant proteus (p.) mira ...201021034629
brain abscesses during proteus vulgaris bacteremia.proteus vulgaris is only rarely the cause of multiple septic metastases. we describe multiple brain abscesses due to p. vulgaris in an immunocompetent patient successfully treated by antibiotic therapy and colonectomy.201020890628
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