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[proteus syndrome]. 200011057145
integron-located veb-1 extended-spectrum beta-lactamase gene in a proteus mirabilis clinical isolate from vietnam.a clinical isolate of proteus mirabilis lil-1 was obtained from a vietnamese patient hospitalized in paris, france. this isolate was resistant to cephalosporins, and there was marked synergy between cephalosporins and clavulanic acid together with unusual synergy between cefoxitin and cefuroxime. pcr analysis revealed the presence of blaveb-1, an integron-located gene coding for an extended-spectrum beta-lactamase (esbl) identified previously in an escherichia coli isolate mg-1 from vietnam. usi ...200011062188
the binding of proteus mirabilis nonagglutinating fimbriae to ganglio-series asialoglycolipids and lactosyl ceramide.proteus mirabilis is a common opportunistic gram-negative uropathogen that infects the upper urinary tract. we have examined the role of the nonagglutinating fimbriae (naf) of p. mirabilis in mediating bacterial adhesion to cell surface receptors. purified naf of p. mirabilis were demonstrated to bind to a number of glycolipids, including asialo-gm1, asialo-gm2, and lactosyl ceramide (laccer) in solid-phase binding assays and in thin layer chromatography (tlc) overlay assays. furthermore, preinc ...200011068685
isolation using triflic acid solvolysis and identification of n(epsilon)-[(r)-1-carboxyethyl]-n(alpha)-(d-galacturonoyl)-l-lysine as a component of the o-specific polysaccharide of proteus mirabilis o13.an amino acid was released from the o-specific polysaccharide of proteus mirabilis o13 by acid hydrolysis and identified as n(epsilon)-[(r)-1-carboxyethyl]-l-lysine by comparison with the authentic sample. an amide of this amino acid with d-galacturonic acid was isolated from the polysaccharide by solvolysis with anhydrous trifluoromethanesulfonic (triflic) acid and characterised by 1h and 13c nmr spectroscopy. these and published data enabled determination of the full structure of the repeating ...200011072853
[bacterial flora of the urinary tract and its drug sensitivity in children with vesico-ureteral reflux].the authors of the following publication present the results of a research which has been made on the presence of bacterial flora in children with vesico-ureteral reflux. as a first step, an analysis of the bacterial flora was made. then, two pathogens occurring most often were selected their drug-sensitivity was tested. the assessment of the bacterial flora and its drug-sensitivity ware based on urine inoculations. clinical material was provided by 184 children suffering from urinary tract infe ...200011081344
periplasmic localization of a groes homologue in escherichia coli transformed with groesx cloned from legionella-like endosymbionts in amoeba proteus.escherichia coli mc4100 transformed with a groe homologous operon cloned from x-bacteria accumulated large amounts of the gene product when cultured at 30 or 37 degrees c. heat shock for 10-30 min at 42 degrees c or ethanol (5%) shock for 2 h increased groesx levels to about twice that in e. coli grown at 30 degrees c. the subcellular localization of groesx in transformed e. coli was determined by several subcellular fractionation methods, by the analysis of extracted proteins in sds polyacrylam ...200011081576
successful in situ treatment of an infected ascending aortic graft.infection of an ascending aortic prosthesis is a grave complication associated with a high mortality. in most cases, extraanatomic bypass and removal of the infected vascular graft are not possible. furthermore, the standard approach to this problem, which includes excision and replacement or debridement and repair of infected thoracic aortic grafts, carries a high early mortality. we report the successful treatment of this life-threatening complication using a conservative strategy in which the ...200011081915
structural and serological studies of the related o-specific polysaccharides of proteus vulgaris o21 and proteus mirabilis o48 having oligosaccharide-phosphate repeating units.the o-specific polysaccharide chains (o-antigens) of the lipopolysaccharides (lpss) of proteus mirabilis o48 and proteus vulgaris o21 were found to have tetrasaccharide and pentasaccharide repeating units, respectively, interlinked by a glycosidic phosphate. polysaccharides and an oligosaccharide were derived from the lpss by various degradation procedures and studied by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,13c and 1h,31p hmqc experiments. the following rel ...200011082201
in vitro activity of gatifloxacin compared with gemifloxacin, moxifloxacin, trovafloxacin, ciprofloxacin and ofloxacin against uropathogens cultured from patients with complicated urinary tract infections.minimum inhibitory concentrations (mics) of gatifloxacin were compared with those of gemifloxacin, moxifloxacin, trovafloxacin, ciprofloxacin and ofloxacin using an agar dilution method for 400 uropathogens cultured from the urine of urological patients with complicated and/or hospital-acquired urinary tract infections (uti). the collection of strains was made up of enterobacteriaceae (34.5%), enterococci (31.5%), staphylococci (21.2%) and non-fermenting bacteria (12.8%). the antibacterial activ ...200011091042
the structure of the carbohydrate backbone of the core-lipid a region of the lipopolysaccharide from proteus vulgaris serotype o25.the following structure of the lipid a-core region of the lipopolysaccharide (lps) from proteus vulgaris serotype o25 was determined by using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of lps, of the products of alkaline deacylation of the lps, and of the products of lps deamination: [structure: see text] terminal residues of beta-glcnac and beta-kdo (indicated by bold italics) are present alternatively in approximately 3:2 amount, leaving no unsubsti ...200011093709
ocular manifestations in proteus syndrome.we report on the ocular manifestations of a proteus syndrome patient. several of the manifestations are due to severe maldevelopment and malfunction of the neuroretina including strabismus, nystagmus, high myopia, and retinal pigmentary abnormalities. in reviewing the literature, strabismus and epibulbar tumors were recorded most commonly. some articles about presumed proteus syndrome are spurious; these have not been included here. also, because of anecdotal and nonsystematic study of the eye a ...200010861666
cefditoren activity against nearly 1000 non-fastidious bacterial isolates and the development of in vitro susceptibility test methods.cefditoren (formerly me-1206) is an investigational, orally administered cephalosporin ester with bactericidal activity against many gram-positive and -negative organisms. cefditoren potency against nearly 1000 non-fastidious species was determined by national committee for clinical laboratory standards (nccls) reference broth microdilution and standardized disk diffusion methods. against staphylococci, usable cefditoren activity was completely correlated with oxacillin with respect to potency a ...200010863109
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1998). i. susceptibility distribution].the frequencies of bacterial isolation and susceptibilities to antimicrobial agents were investigated on 538 bacterial strains isolated from patients with urinary tract infections (utis) in 9 hospitals during the period of june 1998 to may 1999. of the above bacterial isolates, gram-positive bacteria accounted for 30.3% and gram-negative bacteria accounted for 69.7%. susceptibilities of several isolated bacteria to antimicrobial agents were as follows; against enterococcus faecalis isolated from ...200010868300
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1998). ii. background of patients].clinical background was investigated on 449 patients with urinary tract infections (utis) from whom 591 bacterial strains were isolated in 9 hospitals during the period from june, 1998 through may, 1999. about distribution of age and sex of patients and type of infections, among males, patients less than 50 years old were few, and uncomplicated utis without indwelling catheters was most frequent. among females, patients less than 20 years old were few, and uncomplicated was most frequent. escher ...200010868301
influence of clinical history on airways bacterial colonization in subjects with chronic tracheostomy.patients with chronic tracheostomy are subject to significant bacterial colonization of the airways, a risk factor for respiratory infections. the aim of our study was to verify whether bacterial colonization and humoral immune response in the airways can be influenced by the disease which led to chronic respiratory failure and tracheostomy. thirty-nine clinically stable outpatients with chronic tracheostomy were considered: 24 were affected by chronic obstructive pulmonary disease (copd) (mean ...200010868706
lupene-type triterpenes from periploca aphylla.two new lupane derivatives, 3beta,6alpha-dihydroxylup-20(29)-ene (1) and 6alpha-hydroxylup-20(29)-en-3beta-octadecanoate (2), have been isolated from the stems of periploca aphylla, in addition to beta-sitosterol and lupeol. the structures of 1 and 2 were determined by spectral and chemical methods. compound 1 showed strong inhibition of alpha-glucosidase type vi and a moderate antibacterial activity.200010869229
development of an operational substrate for zapa, a metalloprotease secreted by the bacterium proteus mirabilis.the protease zapa, secreted by proteus mirabilis, has been considered to be a virulence factor of this opportunistic bacterium. the control of its expression requires the use of an appropriate methodology, which until now has not been developed. the present study focused on the replacement of azocasein with fluorogenic substrates, and on the definition of enzyme specificity. eight fluorogenic substrates were tested, and the peptide abz-ala-phe-arg-ser-ala-ala-gln-eddnp was found to be the most c ...200010881051
health evaluation of arctic fox (alopex lagopus) cubs in sweden.hematologic, serum biochemistry, and serum cortisol reference ranges were established and tonsil/rectal bacterial and fecal parasite examinations were performed on 21 wild arctic fox (alopex lagopus) cubs during july 1996. several of the hematologic and serum biochemistry values fell within normal ranges for other wild canids or domestic dogs of the same age class. serum alanine transaminase and creatine phosphokinase values were significantly higher in the youngest cubs. proteus vulgaris and es ...200010884121
[urine culture analysis in children with urinary tract infections treated at pediatric clinical department in bytom in the years 1998-1999].sensitivity of bacterial strains causing urinary tract infections was analysed. high sensitivity of bacteria to nitrofurantoin, guinolones and cephalosporines--mainly of the iii generation, as well as minute sensitivity to penicillins were demonstrated.200010897628
the structure of the core part of proteus penneri strain 16 lipopolysaccharide.the structure of the carbohydrate backbone of the lipid a-core region of the lipopolysaccharide (lps) from proteus penneri strain 16 was determined using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of the lps, and of the products of alkaline deacylation of the lps: formula [see text]. incomplete substitution is indicated by bold italics. all sugars are in the pyranose form, alpha-hep is the residue of l-glycero-alpha-d-manno-hep, alpha-dd-hep is the re ...200010903028
fusion of the tissue factor extracellular domain to a tumour stroma specific single-chain fragment variable antibody results in an antigen-specific coagulation-promoting molecule.solid tumours growing beyond a size of 1-2 mm in diameter induce supporting connective tissue structures, the tumour stroma, comprising activated fibroblasts and newly formed blood vessels, embedded in an extracellular matrix. the selective destruction of this tissue or the inhibition of its function (e.g. tumour neoangiogenesis) may result in the destruction of tumour nodules, thus providing novel opportunities for tumour therapy. our approach aims at an antibody-mediated induction of coagulati ...200010903142
pvuii endonuclease contains two calcium ions in active sites.restriction endonucleases differ in their use of metal cofactors despite having remarkably similar folds for their catalytic regions. to explore this, we have characterized the interaction of endonuclease pvuii with the catalytically incompetent cation ca(2+). the structure of a glutaraldehyde-crosslinked crystal of the endonuclease pvuii-dna complex, determined in the presence of ca(2+) at a ph of approximately 6.5, supports a two-metal mechanism of dna cleavage by pvuii. the first ca(2+) posit ...200010903853
characterization of trans-splicing in euglenoids.we have looked for trans-splicing of nuclear mrnas in several euglenoid species. in cyclidiopsis acus, phacus curvicauda, rhabdomonas costata and menoidium pellucidum we showed that several premrnas chosen at random are matured by a transsplicing process: we identified sl-rna genes whose 5' ends (sls for spliced leader-sequences) were transferred to the 5' extremities of mrnas. the sl-rna genes are located on repeated dna fragments which also encode 5s rrna in p. curvicauda and c. acus. the pote ...200010905424
pathological case of the month. proteus syndrome. 200010807308
alpha1-microglobulin as a marker of proximal tubular damage in urinary tract infection in children.the urinary alpha1-microglobulin (alpha1-m) as a marker of proximal tubular damage was measured in 86 children, aged 3/12 to 12 years, with a diagnosis of urinary tract infection (uti) and fever. all patients had normal glomerular filtration rates (gfr). they were divided into 2 groups: a: with uti and etiological factor e. coli, b: with uti and etiological factor proteus sp. similar measurements of alpha1-m were obtained for a control group of healthy children. an increased mean level serum alp ...200010809416
paronychia and excess granulation tissue of the toes and finger in a patient treated with indinavir. 200010809885
molecular mimicry between hla-dr alleles associated with rheumatoid arthritis and proteus mirabilis as the aetiological basis for autoimmunity.molecular mimicry is one of the pathological mechanisms proposed to explain the association between microorganisms and autoimmune diseases. this review deals with the association between bacteria and rheumatic diseases with a special emphasis on rheumatoid arthritis where upper urinary tract infection by proteus mirabilis is the possible cause of this severe, arthritic condition. prospective trials involving anti-proteus therapy should be carried out.200011099935
pathogenesis of proteus mirabilis urinary tract infection.proteus mirabilis is a causative agent of cystitis and pyelonephritis primarily in individuals with indwelling catheters or structural abnormalities of the urinary tract. the organism produces a variety of unique virulence factors that contribute to its pathogenicity and persistence in the human host.200011099936
cost-effective and rapid presumptive identification of gram-negative bacilli in routine urine, pus, and stool cultures: evaluation of the use of chromagar orientation medium in conjunction with simple biochemical tests.the algorithm for a new identification system was designed on the basis of colony color and morphology on chromagar orientation medium in conjunction with simple biochemical tests such as indole (ind), lysine decarboxylase (ldc), and ornithine decarboxylase (odc) utilization tests with gram-negative bacilli isolated from urine samples as well as pus, stool, and other clinical specimens by the following colony characteristics, biochemical reactions, and serological results: pinkish to red, ind po ...200011101600
the structure of the carbohydrate backbone of core-lipid a region ofthe lipopolysaccharides from proteus mirabilis wild-type strain s1959 (serotype o3) and its ra mutant r110/1959.the following structure of core-lipid a region of the lipopolysaccharide (lps) from proteus mirabilis strain 1959 (serotype o3) and its rough mutant r110/1959 (proteus type ii core) was determined using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of lps, and of the products of alkaline deacylation of the lps: incomplete substitutions are indicated by italics. all sugars are in pyranose form, alpha-hep is the residue lglycero-alpha-dmanno-hep, alpha-dd- ...200010601875
production of a tem-24 plasmid-mediated extended-spectrum beta-lactamase by a clinical isolate of pseudomonas aeruginosa.several pseudomonas aeruginosa strains, including one urinary isolate producing an extended-spectrum beta-lactamase tem-24, were isolated from a long-term-hospitalized woman. three tem-24-producing enterobacterial species (enterobacter aerogenes, escherichia coli, and proteus mirabilis) were isolated from the same patient. tem-24 and the resistance markers for aminoglycosides, chloramphenicol, and sulfonamide were encoded by a 180-kb plasmid transferred by conjugation into e. coli hb101.200010602754
convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds.a simple disk diffusion test was constructed for detection of imp-1-type metallo-beta-lactamase-producing gram-negative bacteria. two kirby-bauer disks containing ceftazidime (caz) and a filter disk containing a metallo-beta-lactamase inhibitor were used in this test. several imp-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and edta were evaluated for this test. two caz disks were placed on a mueller-hinton agar plate on which a bacterial suspension ...200010618060
immediate and long-term galvanotactic responses of amoeba proteus to dc electric fields.the long-term and immediate galvanotactic responses of amoeba proteus to the direct current electric fields (dcefs) were studied with the methods of computer-aided image analysis. it was found that in contrast to earlier reports, amoebae continued locomotion towards cathode (the negative pole) for hours and the increase in the field strength in the range 300-600 mv/mm caused the straightening of cell trajectories accompanied by the decreased frequency of the lateral pseudopods formation and less ...200010618163
introduction of a mini-gene encoding a five-amino acid peptide confers erythromycin resistance on bacillus subtilis and provides temporary erythromycin protection in proteus mirabilis.a 15-bp mini-gene was introduced into bacillus subtilis and into stable protoplast-like l-forms of proteus mirabilis. this mini-gene encoded the peptide mvlfv and modeled a fragment of escherichia coli 23s rrna responsible for e. coli erythromycin (ery) resistance. expression of the introduced mini-gene conferred permanent ery resistance on b. subtilis. in l-forms of p. mirabilis, the ery-protective effect was maintained in the course of several generations. herewith, the mechanism of ery resist ...200010620668
synthesis of cefminox by cell-free extracts of streptomyces clavuligerus.in vitro synthesis of cefminox by cell-free extracts of streptomyces clavuligerus was investigated using alpha-ketoglutarate, l-ascorbic acid, feso(4), s-adenosyl-l-methionine, and 7alpha-demethoxycefminox as the substrates. the formation of cefminox was detected both by a biological assay with proteus vulgaris gn 76/c-1 and by high performance liquid chromatography. although the conversion rate of 7alpha-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the poten ...200010620685
aortic ring abscess and aortoatrial fistula complicating fulminant prosthetic valve endocarditis due to proteus mirabilis. 200010625192
insertional transposon mutagenesis by electroporation of released tn5 transposition complexes.dna transposition is an important biological phenomenon that mediates genome rearrangements, inheritance of antibiotic resistance determinants, and integration of retroviral dna. transposition has also become a powerful tool in genetic analysis, with applications in creating insertional knockout mutations, generating gene-operon fusions to reporter functions, providing physical or genetic landmarks for the cloning of adjacent dnas, and locating primer binding sites for dna sequence analysis. dna ...200010625401
dynamic aspects of the structured cell population in a swarming colony of proteus mirabilis.proteus mirabilis forms a concentric-ring colony by undergoing periodic swarming. a colony in the process of such synchronized expansion was examined for its internal population structure. in alternating phases, i.e., swarming (active migration) and consolidation (growth without colony perimeter expansion), phase-specific distribution of cells differing in length, in situ mobility, and migration ability on an agar medium were recognized. in the consolidation phase, the distribution of mobile cel ...200010629184
role and mechanism of action of c. pvuii, a regulatory protein conserved among restriction-modification systems.the pvuii restriction-modification system is a type ii system, which means that its restriction endonuclease and modification methyltransferase are independently active proteins. the pvuii system is carried on a plasmid, and its movement into a new host cell is expected to be followed initially by expression of the methyltransferase gene alone so that the new host's dna is protected before endonuclease activity appears. previous studies have identified a regulatory gene (pvuiic) between the dive ...200010629196
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 63 from a new serogroup o68.lipopolysaccharide of proteus penneri strain 63 was degraded by mild acid to give a high molecular mass o-specific polysaccharide that was isolated by gel-permeation chromatography. sugar and methylation analyses and nmr spectroscopic studies, including two-dimensional 1h, 1h cosy, tocsy rotating-frame noe spectroscopy, h-detected 1h,13c and 1h,31p heteronuclear multiple-quantum coherence (hmqc), and 1h, 13c hmqc-tocsy experiments, demonstrated the following structure of the polysaccharide: wher ...200010632731
rapid turnover of flhd and flhc, the flagellar regulon transcriptional activator proteins, during proteus swarming.the enterobacterial flhdc master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. during proteus mirabilis differentiation into elongated hyperflagellated swarm cells, flhdc transcription is strongly but transiently increased. we show that concentration of the flhd and flhc proteins is also tightly controlled at the posttranslational level. this is achieved by protein degradation, which is most severe after differentiation when the half-lif ...200010633123
how do intestinal t cells sense the dietary and microbial environment? 200010648476
fragmentation of 23s rrna in strains of proteus and providencia results from intervening sequences in the rrn (rrna) genes.intervening sequences (ivss) were originally identified in the rrl genes for 23s rrna (rrl genes, for large ribosomal subunit, part of rrn operon encoding rrna) of salmonella enterica serovars typhimurium lt2 and arizonae. these sequences are transcribed but later removed during rnase iii processing of the rrna, resulting in fragmentation of the 23s species; ivss are uncommon, but have been reported in at least 10 bacterial genera. through pcr amplification of ivs-containing regions of the rrl g ...200010648538
biosynthetically diverse compounds from a saltwater culture of sponge-derived aspergillus niger.the new compound, asperic acid (1), and the known compounds hexylitaconic acid (2), malformin c (3), pyrophen (4), and asperazine (5) were isolated from the saltwater culture of aspergillus niger derived from a caribbean sponge, hyrtios proteus. the structure elucidation of asperic acid is presented.200010650076
structure of a glycerol teichoic acid-like o-specific polysaccharide of proteus vulgaris o12.a phosphorylated o-specific polysaccharide (o-antigen) was obtained by mild acid degradation of proteus vulgaris o12 lipopolysaccharide and studied by sugar and methylation analyses, 1h-, 13c- and 31p-nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, h-detected 1h, 13c and 1h, 31p heteronuclear multiple-quantum coherence experiments. it was found that the polysaccharide consists of pentasaccharide repeating units connected via a glycerol phosphate group, and has the following struc ...200010651815
structure of the o-specific polysaccharide of proteus penneri 71 and classification of cross-reactive p. penneri strains to a new proposed serogroup o64.a neutral o-specific polysaccharide (o-antigen) was isolated from the lipopolysaccharide (lps) of the bacterium proteus penneri 71. on the basis of sugar analysis and 1h- and 13c-nmr spectroscopic studies, including two-dimensional cosy, 13c,1h heteronuclear cosy and roesy, the following structure of the trisaccharide repeating unit of the polysaccharide was established: -->3)-beta-d-glcpnac-(1-->4)-beta-d-glcpnac-(1-->3)-alpha-d-galp-(1-- > the polysaccharide has the same carbohydrate backbone ...200010651818
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 8 from a new serogroup o67.an acidic o-specific polysaccharide was obtained by mild acid degradation of the proteus penneri 8 lipopolysaccharide and found to contain d-glucose, d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, 2-acetamido-2,6-dideoxy-l-galactose (l-fucnac) and 2-aminoethyl phosphate (petn) in the ratios 2 : 1 : 1 : 1 : 1 : 1. 1h and 13c nmr spectroscopy was applied to the intact and dephosphorylated polysaccharides, and the following structure of the hexasaccharide repea ...200010651819
functional morphology of the inner ear and underwater audiograms of proteus anguinus (amphibia, urodela).octavolateral sensory organs (auditory and lateral line organs) of cave salamander proteus anguinus are highly differentiated. in the saccular macula of the inner ear the complex pattern of hair cell orientation and the large otoconial mass enable particle displacement direction detection. additionally, the same organ, through air cavities within the body, enables detection of underwater sound pressure changes thus acting as a hearing organ. the cavities in the lungs and mouth of proteus are a r ...200010653179
the ultrastructure of photoreceptor cells in the pineal organ of the blind cave salamander, proteus anguinus (amphibia, urodela).we studied ultrastructure of the photoreceptor cells in the pineal organ of blind, depigmented, neotenic cave salamander, proteus anguinus. unlike in epigean vertebrates the outer segments of most photoreceptor cells consists of concentrically arranged lamellae, however; in few cells, the outer segments contain 7-9 plasma membrane disks. in both types of photoreceptor cells the outer segments enclose lumps of vesicles of different sizes. the photoreceptor cells of proteus anguinus are similar to ...200010653183
problems related to determination of mics of oximino-type expanded-spectrum cephems for proteus vulgaris.during in vitro susceptibility testing of clinical isolates of proteus vulgaris, we noted that the mics of several expanded-spectrum cephems were much higher in the broth microdilution method than in the agar dilution method (termed the mic gap phenomenon). here we investigated the mechanism of the mic gap phenomenon. cephems with the mic gap phenomenon were of the oximino type, such as cefotaxime, cefteram, and cefpodoxime, which serve as good substrates for inducible class a beta-lactamase (cu ...200010655366
[an observation on long-term influence of middle ear bacterial infection on inner ear function and systemic immune reaction].to understand whether long-term inner ear heat shock response related to heat shock protein(hsp70) caused by middle ear bacterial infection and the potential influence on inner ear function.200012768776
[a study on the determination method of biological function of brf].this study was aimed to establish a method for determining the biological function of biological wave regulating factor (brf). proteus mirabilis was used to set up the experimental model. the biological function of the component obtained from wave growth media beneath the bacterial colony rings resulting from bacterial proliferation was determined in biological wave test by analytically microbiological method. the results indicated that brf could enhance biological wave and shorten its period. t ...200012557788
emerging antibiotic resistance among the uropathogens.a total of 18,876 urine samples from patients of clinically suspected uti including asymptomatic pregnant females were analysed, during the year 1997 & 1998. culture positivity rate was found to be 37%. the incidence of proteus was on the rise during the year 1998 especially in hospitalized patients. the antibiotic sensitivity pattern of uropathogens was also analysed to check the emergence of drug resistance. this study has revealed that withdrawal of a particular antibiotic due to high level o ...200011966063
serratia type pore forming toxins.the serratia marcescens hemolysin represents a new type of hemolysin and has been studied in great molecular detail with regard to structure, activation and secretion. it has nothing in common with the pore forming toxins of e. coli type (rtx toxins), the staphylococcus aureus alpha-toxin or the thiol activated toxin of group a beta-hemolytic streptococci (streptolysin o). studies on erythrocytes, eukaryotic cells and artificial black lipid membranes, have shown that the mechanism of pore format ...200012369921
bacteriology of diabetic foot.to study the relative frequency of bacterial isolates cultured from diabetic foot infections and assess their comparative in vitro susceptibility to the commonly used antibacterial agents.200011533815
microbiology of chronic suppurative otitis media with cholesteatoma.to report the result of causative organisms in chronic suppurative otitis media with cholesteatoma.200011369953
pattern of gram negative rods bacteraemia in diabetic patients in ibadan, nigeria.in a study of 100 patients in ibadan between july and december 1995 to evaluate bacteraemia due to gram-negative bacilli, 64% were culture positive, 44 (68.8%) of these yielded gram-negative rods. the isolates were klebsiella species (43.2%), escherichia coli (27.3%), pseudomonas aeruginosa (13.6%), proteus species (11.4%) and bacteroides melaninogenious (4.15%) by standard bacteriological methods. antimicrobial sensitivity results suggested ofloxacin or ceftriaxone with metronidazole as empiric ...200011713990
[treatment of bacterial peritonitis with dachengqi decoction and rhubarb in mice].to observe the antiseptic effect of dachengqi decoction (dcqd) or rhubarb, one of the ingredients of dcqd in mice.200011783340
[study on lumbricus in promoting ligation hemorrhoidectomy postoperative wound healing].to investigate the effect of lumbricus in promoting wound healing of ligation hemorrhoidectomy.200011938859
evaluation of antibacterial activity of belizean plants: an improved method.the influences of medium type, inoculum density, and a cold incubation on antimicrobial assay sensitivity were tested. the largest and most distinct zones were produced using nutrient agar and the 1/10 4 inoculum density for staphylococcus aureus and proteus mirabilis but a 1/10 12 inoculum density for pseudomonas aeruginosa and escherichia coli . the greatest number of zones were detected without cold incubation. using this method, eight plants from belize were screened for antibacterial activi ...200021214435
c-3 substituted lawsonemonoximates of holmium(iii): synthesis, characterization, and antimicrobial activity.a series of five new metal complexes of ho(iii) with c-3 substituted derivatives of lawsonemonexime (2-hydroxy-1,4-naphthalenediene-1-oxime) were synthesized. the compounds were characterized by melting point, elemental analysis, ir spectroscopy and magnetic susceptibility. the antimicrobial activity of the compounds were determined by disk diffusion method and broth micro-dilution techniques using mueller hinton medium against the following organisms: s. aureus atcc 6538p, klebsiella pneumoniae ...200018475938
[low-intensity laser radiation in preoperative preparation of patients with benign prostatic hyperplasia].low-intensity laser therapy administered in the form of intravenous blood irradiation, transrectal and transurethral prostatic irradiation and their combination as preoperative preparation and correction of immunity disturbances in patients with benign prostatic hyperplasia (bph) were studied. the response to the treatment was evaluated by positive changes in the immune status and bacterial contamination of the urine and prostatic tissue. conventional preoperative preparation (uroantiseptics, an ...200016856453
antimicrobial effects of propolis extracts on escherichia coli and staphylococcus aureus strains resistant to various antibiotics and some microorganisms.abstract propolis is collected by worker honey bees from the trees. the bees pack propolis on their hind legs, carry it to their colony, and use it as a sterilant in the hive. propolis has also been used by humans since early times for various purposes and, especially, as a medicine because of its antimicrobial properties. in this study, antimicrobial effects of propolis samples on three escherichia coli and two staphylococcus aureus strains that are multiresistant to antibiotics and on salmonel ...200019236174
the effect of marigolds (tagetes spp.) and other cover crops on pratylenchus penetrans and on following potato crops.root-lesion nematodes (primarily pratylenchus penetrans) were monitored in two marigold cultivars (tagetes tenuifolia cv. nemakill and cv. nemanon), annual ryegrass (lolium multiflorum cv. lemtal), red clover (trifolium pratense cv. florex), and soybean (glycine max cv. proteus), and in the following potato (solanum tuberosum cv. superior) crop during three growth sequences. meadow fescue (festuca elatior cv. miner) and bee plant (phacelia tanacetifolia cv. gipha) were added to the trial in the ...200019271006
synthesis, characterization and antimicrobial activity of d metal complexes of some 2-substituted-1h-benzimidazoles.the metal complexes of nine 2-substituted-1h-benzimidazoles (i-ix) with ni(ii), pd(ii), cu(ii), ag(i), zn(ii) salts were synthesized. the compounds were characterized by melting point, analytical data, ir spectroscopy and magnetic susceptibility. the antimicrobial activity of the compounds was determined by the disk diffusion method in mueller-hinton agar on staphylococcus aureusatcc 6538, staphylococcus epidermidis atcc 12228, escherichia coli atcc 8739, klebsiella pneumoniae atcc 4352, pseudom ...199918472907
increasing incidence of resistance to antimicrobials in sidamo.this study compares the prevailing sensitivity of bacterial isolates to common antimicrobials during the two-year 1985-87, 1990-92 and 1996-97 periods at yirga alem hospital in southern ethiopia. all specimens were from patients attending the hospital. we studied 1371 specimens, 337,671 and 363 specimens from the periods 1985-87, 1990-92 and 1996-97, respectively. the study confirms earlier observations of widespread resistance to commonly used antibiotics. significant increases in the rate of r ...199911957315
chronic suppurative otitis media in a children's hospital in addis ababa, ethiopia.our knowledge of chronic suppurative otitis media is scanty in ethiopia. this hospital-based study was, thus, conducted prospectively over a period of 2 years among children visiting a tertiary facility in addis ababa. demographic, clinical, audiometric and microbiological data were collected using a preformed questionnaire. a total of 391 patients constituting 0.6% of the hospital patient population and 22.3% of those seen at the ear, nose, and throat clinic had chronic suppurative otitis media ...199911961874
in vitro susceptibility of new generation of antibacterial antibiotics against pathogenic bacteria.antibiotic susceptibility of ten bacteria i.e. neisseria catarrhalis, salmonella typhi, s. enteritidis, haemophilus influenzae, bacillus subtilis, pseudomonas fluorescence, pseudomonas aeruginosa, proteus vulgaris, staphylococcus aureus and e. coli to twenty antibiotics i.e. cefpirom (30 mcg), ceftriaxone (30 mcg), erythromycin (15 mcg), doxycycline (30 mcg) lomefloxacin (10 mcg), sisomicin (30 mcg), vancomycin (30 mcg), augmentin (30 mcg), ampicillin (30 mcg), cotrimoxazole (25 mcg), cefotaxime ...199912024980
intestinal protozoa and associated bacteria in captive houbara bustards (chlamydotis undulata) in the united arab emirates.a protozoal survey was carried out in 114 captive houbara bustards (chlamydotis undulata) in the united arab emirates. trichomonas gallinarum, chilomastix gallinarum, giardia spp. and lophomonas spp. were detected from faecal samples of 49 (43%) captive houbara bustards. culture and identification studies were carried out to assess associated bacteria. the bacteria isolated from birds negative to protozoa included enterococcus spp., aerococcus spp., micrococci spp., proteus spp., enterobacter sp ...199916147554
photoproduction of hydrogen from raw starch using a halophilic bacterial community.the bacterial community designated bc1, which originates from night soil treatment sludge, exhibited a strong ability to produce h2 from raw starch in the light in the presence of 3% nac1. three halophilic or halotolerant bacterial species, vibrio fluvialis, rhodobium marinum, and proteus vulgaris, were isolated from bc1 and identified. the level of h2 production from starch by coculture of v. fluvialis and r. marinum was nearly equal to that by bc1, indicating that these two strains play roles ...199916232577
[five cases of purple urine bag syndrome in a geriatric ward].we report five cases of purple urine bag syndrome (pubs). all the patients were women and they had been bed-ridden for a long period due to cerebrovascular diseases. they tended to be constipated as a result of habitual use of laxatives. indicanuria was proven in the all urinary samples from the patients. the four assessable urinary cultures showed proteus mirabilis contamination. total days without evacuation per month in patients with pubs and control subjects (5 catheterized subjects without ...199910655742
use of polymerase chain reaction to detect proteus mirabilis and ureaplasma urealyticum in urinary calculi.in this study, we evaluated the efficacy of the polymerase chain reaction (pcr) in detecting urea-splitting microorganisms in desiccated urinary tract infection stones. seventy-eight urinary tract stones were tested for the presence of proteus mirabilis and ureaplasma urealyticum by means of pcr with species-specific primers. twenty-seven stone samples were composed of struvite and/or carbonate apatite (infection stone); 40 were calcium oxalate and/or calcium phosphate; seven were mixed, with st ...199910634025
[weil-felix test]. 199910635793
[sepsis and antibiotic prophylaxis in stereotaxic neurosurgery].stereotaxic surgery is becoming increasingly important because of the possibility of approaching the deep zones of the brain with less risk. it is in daily use in cerebral tumours and in the functional surgery of parkinson's disease. the use of antibiotic prophylaxis in neurosurgery is controversial, although in many centres, including ours, all patients receive it.199910637862
overgrowth syndromes: an update. 199910645472
[the results of a multicenter study of the comparative activity of cefepime and other antibiotics against the causative agents of severe hospital infections (the micromax program)].a multicentre trial was performed on the activity of cefepime in comparison with ceftazidime, ceftriaxone, piperacillin/tazobactam, imipenem and ciprofloxacin against severe hospital infection pathogens in intensive care units. the isolates of escherichia coli and proteus spp. from the majority of the centres were highly susceptible to the antibiotics (90 to 100 per cent of the isolates). in some centres up to 40 per cent of the isolates produced esbl. the isolates of klebsiella spp. were charac ...199910629736
sub-mic ciprofloxacin effect on fimbrial production by uropathogenic escherichia coli strains.the urine from 210 patients with acute urinary tract infection (uti) was examined to study the in vitro effect of ciprofloxacin on fimbriae production by uropathogenic escherichia coli isolates. forty-nine bacterial samples of density 10(5) cfu/ml were not considered. from the resulting 161 samples, e. coli was the major strain found, present in 54 samples. other microoganisms found were: enterococcus sp. (34 samples), staphylococcus epidermis (22), yeasts (11), proteus sp. (11), pseudomonas sp. ...199910632381
intestinal protozoa in hiv-infected patients: effect of rifaximin in cryptosporidium parvum and blastocystis hominis infections.in hiv-1 infected patients severe enteritis and chronic diarrhea are often documented as a consequence of multiple opportunistic infections. we analyzed 48 hiv-1 positive patients for the presence of intestinal pathogenic protozoa. patients with cd4 > or = 200/mm3 showed a higher prevalence of a single pathogenic protozoa than patients with cd4 < or =200/mm3, who showed the presence of multiple protozoal infections. patients who proved positive for only a single protozoa, cryptosporidium or blas ...199910632386
disk diffusion susceptibility test development for the new glycylcycline, gar-936.the in vitro activity of gar-936, a new semisynthetic glycylcycline, was evaluated in comparison with two tetracyclines and the disk diffusion susceptibility test was assessed. nearly 700 recent clinical isolates were tested by reference broth microdilution and disk diffusion (two disk contents) methods. among the enterobacteriaceae, gar-936 was generally two- to 16-fold more active than other tetracyclines. all enteric bacilli mic90 results were < or = 4 micrograms/ml; the exception being prote ...199910626138
[surgical policy in false aneurysm of proximal anastomosis secondary to reconstruction of aortofemoral segment].the results of surgical treatment of 10 patients with a false aneurysm of the proximal anastomosis are analyzed. an average time for development of a false aneurysm made up 57.2 months. fluorlon-lavsan vascular woven prosthesis of russian produce was used in primary reconstruction. bacteriological examination of operation material was carried out in 9 patients. 8 cases revealed the growth: of staph. aureus (4) epidermal staphylococcus flora (3) and proteus vulg. (1). the authors suggest that sur ...199910626379
stimulatory effects of endotoxin on the platelet secretory process.the effects of the lipopolysaccharides (lps) of proteus mirabilis (smooth and rough types), differing significantly in their composition on the release of compounds stored in specific platelet granules, were studied. there are two main types of secretory granules in blood platelets. dense granules contain adenine nucleotides, and in alpha-granules different proteins are stored. the lps were found to cause a dose-dependent release of proteins and adenine nucleotides. in the extracellular medium l ...199910624010
isolation and antibacterial activity of phenylpropanoid derivatives from ballota nigra.in addition to the previously isolated phenylpropanoid glycosides verbascoside 1, forsythoside b 2, arenarioside 3 and ballotetroside 4, another four compounds were isolated from generative aerial parts of ballota nigra: three phenylpropanoid glycosides, alyssonoside 5, lavandulifolioside 6 and angoroside a 7 and a non-glycosidic derivative (+)-(e)-caffeoyl-l-malic acid 8. the antibacterial activity of the five major compounds (1-4 and 8) was tested against gram-positive and gram-negative bacter ...199910619384
bacterial populations contaminating the upper gut in patients with small intestinal bacterial overgrowth syndrome.small intestinal bacterial overgrowth syndrome (sibos) is characterized by an abnormally high bacterial population level in the upper gut, exceeding 10(5) organisms/ml (5 log colony-forming unit (cfu)/ml). to understand its origin and select an appropriate antibiotic treatment, we have analyzed the bacterial populations contaminating the upper gut in sibos patients.199910235214
proteus syndrome: craniofacial and cerebral mri.the proteus syndrome is a rare hamartoneoplastic syndrome that may affect the brain, skull, and extracranial head and neck. we present a case with severe, characteristic findings. brain abnormalities are not common in proteus syndrome; when present, hemimegalencephaly and migrational disorders are typically seen, commonly with an associated seizure disorder. maxillary and mandibular dysmorphism may occur, including unilateral condylar hyperplasia. subcutaneous fatty, fibrous, lymphangiomatous ma ...199910602859
[erythrocytic glucose-6-phosphate dehydrogenase deficiency, hemolysis and diabetic ketoacidosis]. 199910603677
comparative evaluation of five chromogenic media for detection, enumeration and identification of urinary tract pathogens.five chromogenic agar plates--cps id2 medium (biomérieux, france), chromagar orientation medium (becton dickinson, france), uriselect3 medium (sanofi diagnostics pasteur, france), rainbow agar uti medium (biolog, usa) and chromogenic uti medium (oxoid, germany)--for the detection, enumeration and direct identification of urinary tract pathogens were compared using 443 urine specimens at two hospital laboratories. the enumeration of microorganisms was consistent on the five media for 403 of the 4 ...199910614954
microbes and sequestered substances as mechanisms for disease: bartonella and l-forms as common global etiological agents.the pathogenicity of microbes may be determined by substances sequestered from blood and bound to their constituent lipid. the brain may not perceive substances sequestered by microbes, to interfere with control to maintain normal levels. pathological conditions can be induced as organisms exposed to antimicrobial substances/conditions and/or deprived of nutrients essential to cell wall synthesis, disintegrate to free lipid-bound compounds and produce l-forms that can deplete nutrients as they r ...199910465665
in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group.we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ...199910467641
investigation of influence of proteus mirabilis lps polysaccharide part on the murine immune cells activation.the biological activities were investigated of proteus mirabilis lipopolysaccharides (lpss) and their fragments, namely, the induction of nitric oxide (no) and interleukin 1 (il-1) synthesis by murine macrophages and the proliferation of murine spleen cells. the o-specific polysaccharide (f1), core oligosaccharide (f2) and lipid a were as effective as intact lps in stimulating murine macrophages to produce no. il-1 synthesis was also induced by all studied types of endotoxins (s, ra, re) and par ...199910470447
paediatric nosocomial urinary tract infection at a regional hospital.from a total of 26,603 admissions to the paediatric wards, 1360 paediatric nosocomial urinary tract infections (pnuti) were identified during a 5-year retrospective chart review at the sfgh. the ages ranged from 3 days to 13 years, with 46% boys and 54% girls. the highest rates of pnuti per service per 100 admissions were seen in the nursery (11.28) followed by paediatric surgery (2.89) and paediatric medicine (2.86). although the greatest number of pnuti occurred in the nursery, comparison betw ...199910481961
proteus mirabilis osteomyelitis of the ribs in a girl with sickle cell anaemia. 199910485315
canine distemper virus infection in binturongs (arctictis binturong).two binturongs (arctictis binturong) kept in outdoor exhibits at everland zoological gardens in korea died within 10 days of the onset of clinical signs that included depression, dyspnoea, diarrhoea and convulsions. on necropsy, the significant gross findings were limited to the lungs and the gastrointestinal tract. proteus vulgaris was isolated from the lung of one animal. histopathologically, diffuse severe bronchointerstitial pneumonia with secondary bacterial infection was noted in the lungs ...199910486167
the facilitating effect of cigarette smoke on the colonization of instilled bacteria into the tracheal lumen in rats and the improving influence of supplementary vitamin e on this process.the aim of the present study was to investigate the direct influence of cigarette smoke on the bacterial colonization of the lower respiratory tract and the effect of supplementary vitamin e on the colonization of instilled bacteria into the trachea of rats that do not have the chronic airway pathology associated with smoking.199910489666
[development of a vaccine for treatment of urinary tract infections].receiving uroinfections are predominantly treated with antibiotics and chemotherapeutics. an effective alternative treatment can be immunotherapy with inactivated bacteria. the authors have focused on the development of polybacterial vaccine with both specific and nonspecific immunostimulatory activity. the vaccine contains killed bacteria which most frequently occur as an etiological agent in urinary tract infections. determining for the selection of particular strains for the vaccine preparati ...199910493005
[clinical success of treatment of chronic otitis media using topical and peroral administration of ofloxacin].the paper presents results of the treatment of 32 patients (35 ears) with chronic otitis media and long lasting purulent otorrhea with local and oral ofloxacin. the patients were divided in two groups. group a included 17 patients (49%), with previous middle ear surgery. group b comprised 15 patients (18 ears, 51%) treated with local and systemic antimicrobial drugs. ofloxacin was administered after taking the swab sample, and the duration of treatment was the same in all patients. the most freq ...199910494153
genomic subtractive hybridization and selective capture of transcribed sequences identify a novel salmonella typhimurium fimbrial operon and putative transcriptional regulator that are absent from the salmonella typhi genome.salmonella typhi, the etiologic agent of typhoid fever, is adapted to the human host and unable to infect nonprimate species. the genetic basis for host specificity in s. typhi is unknown. the avirulence of s. typhi in animal hosts may result from a lack of genes present in the broad-host-range pathogen salmonella typhimurium. genomic subtractive hybridization was successfully employed to isolate s. typhimurium genomic sequences which are absent from the s. typhi genome. these genomic subtracted ...199910496884
quorum-sensing cross talk: isolation and chemical characterization of cyclic dipeptides from pseudomonas aeruginosa and other gram-negative bacteria.in cell-free pseudomonas aeruginosa culture supernatants, we identified two compounds capable of activating an n-acylhomoserine lactone (ahl) biosensor. mass spectrometry and nmr spectroscopy revealed that these compounds were not ahls but the diketopiperazines (dkps), cyclo(deltaala-l-val) and cyclo(l-pro-l-tyr) respectively. these compounds were also found in cell-free supernatants from proteus mirabilis, citrobacter freundii and enterobacter agglomerans [cyclo(deltaala-l-val) only]. although ...199910510239
antibiotic sensitivity of bacteria associated with community-acquired urinary tract infection in britain.twelve laboratories in different parts of britain each supplied approximately 80 consecutive urinary bacterial isolates from community patients. all strains were identified by a central laboratory, where sensitivity to a variety of orally administered antimicrobials was determined by microtitre broth dilution. 65.1% of isolates were escherichia coli, 23.4% 'coliforms' other than e. coli, 4.6% proteus and morganella spp., 1.8% pseudomonas spp., 2.4% enterococci, 0.7% group b streptococci, 1.5% co ...199910511403
association of rickettsia helvetica with chronic perimyocarditis in sudden cardiac death.rickettsia helvetica is the only non-imported rickettsia found in scandinavia. it was first detected in ixodes ricinus ticks, but has never been linked to human disease. we studied two young swedish men who died of sudden cardiac failure during exercise, and who showed signs of perimyocarditis similar to those described in rickettsial disease.199910513711
in vitro antibacterial activity of fk041, a new orally active cephalosporin.the in vitro activity of fk041, a new orally active cephem antibiotic, against a wide variety of clinical isolates of bacteria was investigated and compared with those of cefdinir (cfdn) and cefditoren (cdtr). fk041 exhibited broad spectrum activity against reference strains of gram-positive and gram-negative aerobes and anaerobes. fk041 was active against clinical isolates of gram-positive organisms except enterococcus faecalis with mic90s less than 1.56 microg/ml. fk041 was more active than cf ...199910513845
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