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novel class a beta-lactamase sed-1 from citrobacter sedlakii: genetic diversity of beta-lactamases within the citrobacter genus.citrobacter sedlakii 2596, a clinical strain resistant to aminopenicillins, carboxypenicillins, and early cephalosporins such as cephalothin, but remaining susceptible to acylureidopenicillins, carbapenems, and later cephalosporins such as cefotaxime, was isolated from the bile of a patient treated with beta-lactam and quinolone antibiotics. the isolate produced an inducible class a beta-lactamase of pi 8.6, named sed-1, which was purified. characterized by a molecular mass of 30 kda, sed-1 pref ...200111451687
symptomatic diffuse colonic lipomatosis in proteus syndrome. 200111454051
the generation of superoxide anion in blood platelets in response to different forms of proteus mirabilis lipopolysaccharide: effects of staurosporin, wortmannin, and indomethacin.lipopolysaccharide (lps), the major component of the outer membrane of gram-negative bacteria may activate blood platelets. the aim of our study was to evaluate the effects of different forms of proteus mirabilis lps and isolated lipid a and polysaccharide part on the production of superoxide radicals in blood platelets and to estimate the role staurosporin, wortmannin and indomethacin on this process. we compared the generation of superoxide radicals in platelets treated with lps after preincub ...200111457473
new antioxidant and antimicrobial ellagic acid derivatives from pteleopsis hylodendron.bioassay-guided isolation of two new compounds, 3,4-methylenedioxy-3'-o-methyl-4'-o-glucoside ellagic acid (1) and the pteleoellagic acid derivative (2), from the stem bark of pteleopsis hylodendron is reported along with 3,4-methylenedioxy-3'-o-methyl ellagic acid (3), 3,3'-di-o-methyl ellagic acid (4) and 3,3',4'-tri-o-methyl ellagic acid (5), which were obtained for the first time from this plant. the structures of these compounds were elucidated with the help of spectroscopic studies. compou ...200111458450
the effects of pentoxifylline treatment on bacterial translocation after hemorrhagic shock in rats.bacterial translocation is thought to be responsible for infectious complications after hemorrhagic shock. the aim of this study was to investigate the effects of pentoxifylline treatment on bacterial translocation in animals subjected to hemorrhagic shock. thirty-one wistar albino rats (280-360 g) were divided into three groups: sham (n=10), shock (n=11), and shock-pentoxifylline (n=10). blood was not withdrawn from sham rats. shock rats were subjected to 30 min of shock followed by reinfusion ...200111467403
interrelations of organism prevalence, specimen collection method, and host age, sex, and breed among 8,354 canine urinary tract infections (1969-1995).selected information was compiled from canine urinalyses and urine cultures conducted between january 1969 and december 1995. eight thousand three hundred fifty-four microbial isolates (bacteria and fungi) included 4,873 isolates from females and 3,481 from males. ten bacterial genera accounted for 96.3% of the urinary isolates, including escherichia coli (44.1%), staphylococcus spp. (11.6%), proteus spp. (9.3%), klebsiella spp. (9.1%), enterococcus spp. (8.0%), and streptococcus spp. (5.4%) as ...200111467591
the effect of ship scrapping industry and its associated wastes on the biomass production and biodiversity of biota in in situ condition at alang.the main pollutants for the ship scrapping industry and its associated wastes at alang are heavy metals, petroleum hydrocarbon and bacterial contaminations. the concentration of iron, manganese, cobalt, copper, zinc, lead, cadmium, nickel and mercury were 25 to 15,500% more at nearshore station of alang as compared to control site at piram. the concentration of heavy metals in the nearshore station of alang was always higher than its concentration at 10 km away. the concentration of petroleum hy ...200111468924
asymmetry: molecular, biologic, embryopathic, and clinical perspectives.this overview of asymmetry addresses the following topics: chiral molecules; asymmetric signaling molecules, including n-cadherin, shh, fgf8, lefty1, lefty2, nodal, pitx2, activin betab, activin receptor iia, and csnr; situs abnormalities; asymmetric cell division; laterality in humans and animals; behavioral asymmetry in humans and animals; asymmetric embryopathies, including tessier-type "clefts"; hemiasymmetries such as hemihyperplasia, hemihypoplasia, and hemiatrophy; asymmetric vascular syn ...200111471152
the formulation of an effective topical antibacterial product containing ocimum gratissimum leaf essential oil.the antibacterial potential of ocimum gratissimum essential oil was explored. liquid and semisolid formulations of the oil were designed in a variety of bases for topical antiseptic medication. the products were evaluated by agar diffusion assay against type strains and clinical isolates from boil, wound and pimples. remarkable antibacterial effects, higher than those of commercial antiseptic products, were demonstrated at 2% ocimum oil concentration in some bases. the properties of base into wh ...200111472827
advances in disinfection testing and modelling.to develop a set of kinetic equations which more ably describe the disinfection process.200111473601
[black nails caused by proteus mirabilis].black nails caused by proteus mirabilis were seen in a motor mechanic and in a petrol pump attendant. proteus mirabilis is a gram-negative bacillus that generates hydrogen sulfide. this compound reacts with traces of metals in the nail plate such as zinc, nickel, cobalt, iron, manganese, tin, copper and lead. metal sulfides blacken the nail plate. the protracted course of the discoloration over months corresponds to the slow reactions of metals with hydrogen sulfide. the disappearance of the bla ...200111475651
blood stream infections in a medical intensive care unit: spectrum and antibiotic susceptibility pattern.to determine the type and sensitivity pattern of causative organisms of septicaemia in intensive care unit, to prepare a guideline for empirical antibiotic therapy.200111475775
pten mutations are uncommon in proteus syndrome. 200111476065
association of germline mutation in the pten tumour suppressor gene and proteus and proteus-like syndromes.the molecular aetiology of proteus syndrome (ps) remains elusive. germline mutations in pten cause cowden syndrome and bannayan-riley-ruvalcaba syndrome, which are hereditary hamartoma syndromes. some features-eg, macrocephaly, lipomatosis, and vascular malformations-can be seen in all three syndromes. we examined pten in patients with ps and undefined proteus-like syndromes (ps-like) and identified de-novo germline mutations in two of nine patients with ps and three of five patients with ps-lik ...200111476841
etiologies of the urinary tract infections in a yemeni city.to determine the causative agents of urinary tract infection in yemeni patients in sana'a city, and to determine the antibiotic susceptibilities of these agents in vitro.200111479641
coronal leakage following three obturation techniques.to compare coronal bacterial and india ink leakage in three different obturation techniques with the smear layer having been removed.200111482141
antimicrobial activity of alstonia macrophylla: a folklore of bay islands.the methanolic crude and methanol-aqueous extract of alstonia macrophylla leaves and n-butanol part of the crude extract showed antimicrobial activity against various strains of staphylococcus aureus, staphylococcus saprophyticus, streptococcus faecalis, escherichia coli, proteus mirabilis, trichophyton rubrum, trichophyton mentagrophytes var. mentagrophytes and microsporum gypseum. the minimum inhibitory concentration (mic) values ranges from 64 to 1000 microg/ml for bacteria and 32-128 mg/ml f ...200111483378
dental unit waterlines: review and product evaluation.dental practitioners must be knowledgeable regarding microbial contamination and biofilm formation in dental unit waterlines. education should stress the need for improvement in the quality of water delivered to patients during treatment. manufacturers must also play an important role by providing training and education regarding the proper use and maintenance of their systems. dental facilities, both public and private, need reliable methods to prevent the development of biofilms within duws. t ...200111484611
intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae.intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ...200111544246
identification and localisation of caldesmon-like protein in amoeba proteus. 200111544656
effects of blocking of endogenous rho family gtp-binding proteins on morphology, adhesion and locomotion of amoeba proteus. 200111544664
filamentous structures in amoeba proteus visualized in scanning microscope. 200111544682
[humoral immune response in patients with bronchopulmanory infections to immunotherapy].the content of iga, igm, igg and the level of specific antibodies in the blood serum of patients with bronchopulmonary diseases after a course of immunotherapy with polycomponent vaccine b[symbol: see text]-4 was studied. a rise in the concentration of igm due to the synthesis of specific antibodies to proteus vulgaris, escherichia coli and klebsiella pneumoniae was found to occur. the examination of sick children revealed that a high proportion of them (54%) showed a pronounced decrease in the ...200111548259
[experimental study of photodynamic effect on bacterial wound microflora].the in vitro study of the influence of photodynamic action (with the use of photosensitizer "photosens", laser and non-laser irradiation) on the strains of the main representatives purulent wound microflora was carried out. staphylococcus aureus, s. epidermidis, pseudomonas aeruginosa, escherichia coli, proteus mirabilis cultures have been isolated from wound secretions and identified. the photosensitizer was shown to produce no bactericidal effect by itself. irradiation with laser and non-laser ...200111548270
new aspects of the role of mr/p fimbriae in proteus mirabilis urinary tract infection.proteus mirabilis, a common cause of urinary tract infection (uti), produces a number of different fimbriae including mannose-resistant proteus-like fimbriae (mr/p). the precise role of different p. mirabilis fimbriae in ascending uti has not yet been elucidated. in this study, a clinical isolate of p. mirabilis and an isogenic mutant unable to express mr/p were tested using different experimental approaches. they were tested for their ability to cause infection in an ascending co-infection mode ...200111549418
[intestinal microbiocenosis in children with intestinal enzymopathy].141 children with different kinds of intestinal enzymopathy were examined; of these, 33 had celiac disease, 39--the syndrome of celiac disease, 12--congenital lactase deficiency and 57--the syndrome of disaccharidase insufficiency. in these patients a significant decrease in the average characteristics of the main protective flora and the growth of hemolytic and lactose-negative enterobacteria were established. in all groups of patients increased amounts of proteus were detected, which was indic ...200111550576
involvement of coenzyme a esters and two new enzymes, an enoyl-coa hydratase and a coa-transferase, in the hydration of crotonobetaine to l-carnitine by escherichia coli.two proteins (caib and caid) were found to catalyze the reversible biotransformation of crotonobetaine to l-carnitine in escherichia coli in the presence of a cosubstrate (e.g., gamma-butyrobetainyl-coa or crotonobetainyl-coa). caib (45 kda) and caid (27 kda) were purified in two steps to electrophoretic homogeneity from overexpression strains. caib was identified as crotonobetainyl-coa:carnitine coa-transferase by maldi-tof mass spectrometry and enzymatic assays. the enzyme exhibits high cosubs ...200111551212
[activity and thermal stability of acid phosphatase in homogenates of amoeba proteus, acclimated to various temperatures].activity and thermoresistance of acid phosphatase were determined in supernatant of amoeba proteus homogenates using 1-naphthyl phosphate (ph 4.0) and p-nitrophenyl phosphate (ph 5.5). although tartrate-resistant and tartrate-sensitive acid phosphatases hydrolyse both substrates, the former mainly hydrolyses p-nitrophenyl phosphate and the latter 1-naphthyl phosphate. a decrease in the activity of the total and tartrate-sensitive acid phosphatases, when using 1-naphthyl phosphate, and of the tot ...200111552654
identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa.the bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after d-galactosamine-induced liver injury, with and without pretreatment with lactobacillus plantarum dsm 9843, were studied in the rat. dominating representatives were identified to species level by 16s rdna sequencing and typed by randomly amplified polymorphic dna (rapd) and by restriction endonuclease analysis (rea) for strain definition. it was proven that bacterial strai ...200111552954
isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis.the aim of this study was to determine the occurrence and role of yeasts, enteric gram-negative rods and enterococcus species in root-filled teeth with chronic apical periodontitis, and the antimicrobial effect of iodine potassium iodide (iki) irrigation.200111556508
proteus syndrome: a case report.proteus syndrome is a rare genetic disorder, characterized by partial gigantism of the hands and/or feet, asymmetry of the limbs, plantar hyperplasia, multiple hamartomatous subcutaneous tumors, hyperostoses, and long bone overgrowth. a one day old thai male infant is reported with macrosomia, hemihypertrophy of the left side of the face and left leg, large feet, macrodactyly of toes, plantar hyperplasia, large subcutaneous mass with a violet-red surface over the left side of the chest wall and ...200111560226
bioactive compounds from iostephane heterophylla (asteraceae).the novel bisabolene sesquiterpenes 3-6, were isolated from iostephane heterophylla, using bioguided fractionation. the new compounds were determined to be (12r/12s)-12,13-epoxy-xanthorrhizols (3,4) and (12r/12s)-12,13-dihydro-12,13-dihydroxy-xanthorrizols (5,6) and their structures were characterized by analysis of spectroscopic data and by chemical correlation from xanthorrhizol (2). the stereochemistry at c-12 of 5 was deduced using the modified mosher experiment. some of the isolated compoun ...200111561451
ligand diffusion in the catalase from proteus mirabilis: a molecular dynamics study.the role of the channels and cavities present in the catalase from proteus mirabilis (pmc) was investigated using molecular dynamics (md) simulations. the reactant and products of the reaction, h(2)o(2) -->1/2 o(2) + h(2)o, catalyzed by the enzyme were allowed to diffuse to and from the active site. dynamic fluctuations in the structure are found necessary for the opening of the major channel, identified in the x-ray model, which allows access to the active site. this channel is the only pathway ...200111567083
scoliosis in proteus syndrome: case report.the case of patient with scoliosis based on a rare hamartomatous, proteus syndrome, is reported.200111568716
gram-negative bacterial toe web infection: a survey of 123 cases from the district of cagliari, italy.foot intertrigo is mostly caused by dermatophytes and yeasts, less frequently by gram-positive and gram-negative bacteria. nevertheless, the importance of polymicrobial infections and especially colonizations of pseudomonas aeruginosa can cause therapy problems in relation to antibiotic resistance and the risk of potentially lethal complications.200111568743
[clinical significance of microbiological monitoring of infective agents in an urological hospital in the selection of antibacterial therapy regimens].individual microbiological examination of each patient can improve significantly the response of urological patients to antibacterial therapy. 6000 cultures of opportunistic microorganisms isolated from patients with various infectious-inflammatory diseases were studied. most of them were gram-negative: p. aeruginosa, e. coli, enterobacter spp., proteus spp. of gram-positive most frequently occurred staphylococcus spp and enterococcus faecalis. the cultures showed different spectrum of the sensi ...200111569227
[formation of intestinal microflora in children during the first year of their life].in 525 young children the state of intestinal microbiocenosis was studied every month of the first year their life. the study revealed that the process of the microflora formation lasted throughout the first year of their life and was characterized by dysbiotic disturbances. during this period the aggravation of dysbiotic changes in the intestine of these children on months 3, 6-7 and 11-12 was of particular importance. the formation of stable dysbacteriosis led to a decrease in the immunologica ...200111569262
electrochemical biosensor array for the identification of microorganisms based on lectin-lipopolysaccharide recognition.rapid identification of bacterial strains remains a well-known problem in applied medicine and, for viable pathogens, is an important diagnostic goal. we have investigated an electrochemical biosensor array, in which transduction is based on respiratory cycle activity measurements, where the microorganism's native respiratory chain is interrupted with non-native external oxidants. the selective biochemical recognition agents employed in this study are lectins that, once immobilized, recognize an ...200111569815
bacterial swarming: a biochemical time-resolved ftir-atr study of proteus mirabilis swarm-cell differentiation.fourier transform infrared spectroscopy was applied to the study of the differentiation process undergone by proteus mirabilis. this bacterium exhibits a remarkable dimorphism, allowing the cells to migrate on a solid substratum in a concerted manner yielding characteristic ring patterns. we performed an in situ noninvasive analysis of biochemical events occurring as vegetative cells differentiate into elongated, multinucleate, nonseptate, and hyperflagellated swarm cells. the major findings ari ...200111570895
[monitoring of uropathogens and their susceptibility to antibiotics].samples of urine collected from patients with complicated urology infection and hospitalized to the moscow region research clinical institute in 1986, 1991, 1995 and 1999 were analysed. of 11,444 samples examined, bacteriuria was estimated in 7143 samples. 9786 strains (29 genus) of bacteria were isolated--56.9 per cent as mono culture and 43.1 per cent as associations. susceptibility to 21 antibiotic was determined by disk diffusion method for 1607 strains; beta-lactamase production was determi ...200111573319
[clinical significance of infection pathogen resistance in a urological clinic for selection of antibacterial therapy regimes for the treatment of complicated urinary infections].data on changes of etiological structure of the complicated urinary tract infections treated at the research institute of urology and hospital no. 47 at the 5-year period (1996-2000) are given. it was shown that the most important microorganisms were the following: enterobacter spp., e. coli, proteus spp., staphylococcus spp., enterococcus faecalis. the correlation between frequency of the microorganisms isolation and their susceptibility to antimicrobial agents at different periods was analysed ...200111573320
rheumatoid arthritis: the future is not yet now. 200111574984
rheumatoid arthritis as an autoimmune disease caused by proteus urinary tract infections: a proposal for a therapeutic protocol. 200111574986
enzymatic characterization of tem-63, a tem-type extended spectrum beta-lactamase expressed in three different genera of enterobacteriaceae from south africa.the extended-spectrum beta-lactamase, tem-63, was identified in three separate genera of south african isolates: proteus mirabilis, klebsiella pneumoniae, and escherichia coli. this paper describes identification of the gene in these isolates and compares relative rates of hyrolysis between tem-63 and other known ceftazidimases.200111576794
isolation and structure elucidation of a new antifungal and antibacterial antibiotic produced by streptomyces sp. 201.an antibacterial and antifungal antibiotic was isolated from the culture filtrate of streptomyces sp. 201, and its structure was determined as 2-methyl-heptyl isonicotinate by extensive use of nmr spectroscopy. the compound exhibited marked antimicrobial activity against bacillus subtilis, shigella sp., klebsiella sp., e. coli, proteus mirabilis, and the pathogenic fungi, fusarium moniliforme, f. semitectum, f. oxysporum, f. solani and rhizoctonia solani.200111577728
proteus syndrome.a case of proteus syndrome is presented, in which severe hemihypertrophy of the left trunk and left lower extremity, scoliosis, endometriosis and huge bizarre-shaped body tumors were observed. up to 22.6 kg of tumorous tissue was excised. this syndrome was first described in 1983. the name proteus comes from a greek mythical sea god who was able to change his body form freely. this syndrome has numerous features including hemihypertrophy, macrodactyly, various subcutaneous masses, scoliosis and ...200111586023
[massive pleural empyema caused by pyonephrosis].the pleural empyema has unusual origin at extralung causes. then, is essential to identify the primary process in order to obtain the clinical setting control. previously described, but even exceptional, this case report show an empyema in which it's origin was an asymtomatic pyonephrosis, caused because of a big kidney calculus and it's discovery was suspected by the bacillus results. the treatment must include appropriate antibiotic therapy and thoracic drainage, and also the drain of the puru ...200111594181
herpes simplex virus type 2 modulates the susceptibility of human bladder cells to uropathogenic bacteria.the present study analyses the susceptibility of human bladder-derived cells (ht-1376) to the infection by herpes simplex virus type 2 (hsv-2) and chlamydia trachomatis, as well as to the adhesiveness of uropathogenic bacteria. ht-1376 cells were efficiently infected by hsv-2 strain 333, as demonstrated by immunofluorescence staining of viral antigens, titration of cytopathic effect, and visualisation by transmission electron microscopy. this cell model was also prone to c. trachomatis (serovar ...200111599790
[identification of vibrio spp bacteria on skin lesions of fisherman in the county of raposa-ma].the study was undertaken aiming at identifying bacteria from the county of raposa in the state of maranhão. the clinical sample was collected by using a swab and held in a cary-blair transport medium. enrichment in alkaline peptone water, isolation in tcbs selective indicator medium and biochemical coding of species were used for laboratory processing. fifty fisherman with age varying from 12-65 years took part on the study. vibrio bacteria isolated in 21 subjects had been identified. there was ...200111600905
identification of bacteria crucial to histamine accumulation in pacific mackerel during storage.bacterial growth and histamine formation in pacific mackerel during storage at 0, 4, 15, and 25 degrees c were monitored. to identify bacterial species contributing to histamine formation, several groups of bacteria were isolated by using selective media under temperatures corresponding to the various storage conditions. initially, low counts of bacteria were found in the gill, skin, and intestine of fresh fish, and only weak histamine formers were found in the gill. histamine was found in the m ...200111601705
zapa, a possible virulence factor from proteus mirabilis exhibits broad protease substrate specificity.the opportunistic bacterium proteus mirabilis secretes a metalloprotease, zapa, considered to be one of its virulence factors due to its iga-degrading activity. however, the substrate specificity of this enzyme has not yet been fully characterized. in the present study we used fluorescent peptides derived from bioactive peptides and the oxidized beta-chain of insulin to determine the enzyme specificity. the bradykinin- and dynorphin-derived peptides were cleaved at the single bonds phe-ser and p ...200111668347
antibiotic susceptibility of bacterial strains isolated from patients with community-acquired urinary tract infections.isolates from urine samples obtained during 1999 were identified and their susceptibility to antimicrobial agents studied along with any production of extended-spectrum beta-lactamases (esbl) by escherichia coli and klebsiella pneumoniae. a total of 13774 samples were analysed using an automatic system for the detection of bacterial atp (coral, usa). of these samples, 49% were reported to be positive and uncontaminated; bacteria most frequently isolated were e. coli (47%), proteus mirabilis (7%) ...200111673032
a european perspective on nosocomial urinary tract infections i. report on the microbiology workload, etiology and antimicrobial susceptibility (esgni-003 study). european study group on nosocomial infections.to obtain information on the microbiology workload, etiology and antimicrobial susceptibility of urinary tract infection (uti) pathogens isolated in european hospitals.200111683792
epidermal naevus and segmental hypermelanosis associated with an intraspinal mass: overlap between different mosaic neuroectodermal syndromes.the epidermal naevus syndrome is a neurocutaneous syndrome characterised by the association of epidermal naevi with central nervous system, skeletal, ocular or cardiovascular abnormalities. we report on a 1.75-year-old boy who presented with spastic diparesis, a partial paresis of the left forearm and macrocephaly. he had a large epidermal naevus along the cervical spine and a segmental hypermelanosis. mri studies revealed a large intraspinal mass extending from the lower cervical to the upper t ...200111686504
evaluation of extracts of the root of landolphia owerrience for antibacterial activity.ethanolic and aqueous (cold and hot) extracts of landolphia owerrience root parts (whole-root, root-bark and root-wood) were tested for activity against ten bacterial strains using agar-well diffusion and macro-broth dilution methods, respectively. the ethanolic extracts of the whole-root and root-wood were active against 100 and 80% of the test organisms, respectively. ethanolic and aqueous extracts of the root-bark were moderately active while the aqueous (cold and hot) extracts of the root-wo ...200111694355
formation of a tyrosyl radical intermediate in proteus mirabilis catalase by directed mutagenesis and consequences for nucleotide reactivity.proteus mirabilis catalase (pmc) belongs to the family of nadph binding catalases. the function of nadph in these enzymes is still a matter of debate. this study presents the effects of two independent phenylalanine mutations (f194 and f215), located between nadph and heme in the pmc structure. the phenylalanines were replaced with tyrosines which we predicted could carry radicals in a nadph-heme electron transfer. the x-ray crystal structures of the two mutants indicated that neither the bindin ...200111695923
cellular membrane associated mucins in artificial urine as mediators of crystal adhesion: an in vitro enterocystoplasty model.cells are a major component of mucus in enterocystoplasties. we evaluate the role of secreted mucins on cells and cellular membranes as crystal adhesion and agglomeration mediators in artificial urine infected with proteus mirabilis.200111696777
analyses of mrp genes during myxococcus xanthus development.myxococcus xanthus is a gram-negative soil bacterium that undergoes development under starvation conditions. our previous study identified a new genetic locus, mrp, which is required for both fruiting body formation and sporulation. the locus encodes two transcripts: mrpab, which consists of a histidine kinase and an ntrc-like response regulator, and mrpc, a cyclic amp receptor protein family transcription activator. in this study, we used genetic and biochemical analyses to investigate the poss ...200111698359
examination of pineal indoles and 6-methoxy-2-benzoxazolinone for antioxidant and antimicrobial effects.oxidative modification of low density lipoprotein (ldl) induced by free radicals is implicated in the development of atherosclerosis. the aim of the present study was to examine the ability of various pineal indoles in inhibiting ldl oxidation which is accompanied by an increase in mobility in agarose gel electrophoresis and by an augmented generation of thiobarbituric acid-reactive substance induced by cu2+. it was found that the order of potencies in inhibiting malondialdehyde formation was 5- ...200111701394
proteus syndrome associated with ocular anomalies. 200111703003
predominant bacterial agents of childhood septicaemia in jos.blood cultures were performed on 891 children (aged 1 month-16 years) who presented at the jos university teaching hospital (juth) with presumed diagnosis of septicaemia, over a three year period (january 1994-december 1996) in order to determine the predominant bacterial agents. one hundred and thirty-nine (15.6%) out of 891 blood cultures were positive. five of the blood cultures yielded double isolates. staph. aureus was the commonest bacterial agent isolated and accounted for 36.0%, of all t ...200111705063
impact of fluoroquinolone administration on the emergence of fluoroquinolone-resistant gram-negative bacilli from gastrointestinal flora.we assessed the risk factors for acquisition of fluoroquinolone-resistant, gram-negative organisms in the gastrointestinal tract of hospitalized patients. we analyzed stool samples from 204 patients and recovered fluoroquinolone-resistant, gram-negative organisms from 63. receipt of fluoroquinolone during the month preceding admission was the only risk factor identified, whereas female sex, duration of hospitalization, exposure to indwelling devices, admission from another hospital, and history ...200111112677
assessment of the capacity of lactobacillus to inhibit the growth of uropathogens and block their adhesion to vaginal epithelial cells.to gain insight into the mechanisms by which lactobacillus blocks the adherence of uropathogens to vaginal epithelial cells and inhibits their growth, 15 lactobacillus strains and 22 uropathogens were studied. lactobacilli from hemagglutination group iii, identified as lactobacillus crispatus, showed greater capacity to block uropathogen adherence than those from hemagglutination groups ii and i (61.9%, 49.5%, and 52.6% of blockage, respectively). pseudomonas aeruginosa pa5 and klebsiella pneumo ...200111133381
behavioural, physiological and metabolic responses to long-term starvation and refeeding in a blind cave-dwelling (proteus anguinus) and a surface-dwelling (euproctus asper) salamander.the effects of long-term starvation and subsequent refeeding on haematological variables, behaviour, rates of oxygen consumption and intermediary and energy metabolism were studied in morphologically similar surface- and cave-dwelling salamanders. to provide a hypothetical general model representing the responses of amphibians to food stress, a sequential energy strategy has been proposed, suggesting that four successive phases (termed stress, transition, adaptation and recovery) can be distingu ...200111136613
conserved regulatory elements of the promoter sequence of the gene rpoh of enteric bacteria.the rpoh regulatory region of different members of the enteric bacteria family was sequenced or downloaded from genbank and compared. in addition, the transcriptional start sites of rpoh of yersinia frederiksenii and proteus mirabilis, two distant members of this family, were determined. sequences similar to the sigma(70) promoters p1, p4 and p5, to the sigma(e) promoter p3 and to boxes dnaa1, dnaa2, camp receptor protein (crp) boxes crp1, crp2 and box cytr present in escherichia coli k12, were ...200111139607
flagellin, a novel mediator of salmonella-induced epithelial activation and systemic inflammation: i kappa b alpha degradation, induction of nitric oxide synthase, induction of proinflammatory mediators, and cardiovascular dysfunction.gram-negative sepsis is mediated by the actions of proinflammatory genes induced in response to microbes and their products. we report that flagellin, the monomeric subunit of flagella, is a potent proinflammatory species released by salmonella. flagellin (1 microgram/ml) induces ikappabalpha degradation, nf-kappab nuclear translocation, and inducible no synthase expression in cultured intestinal epithelial cells (iec). aflagellic salmonella mutants do not induce nf-kappab activation or no produ ...200111145708
catheter-associated urinary tract infections.in the past few years it has been clearly demonstrated that the concept of bacterial biofilm production permits an understanding and provides some explanation of the pathogenesis, diagnosis and treatment of catheter-associated urinary tract infections. this concept describes the colonization of catheter surfaces and the movement of bacteria against the urinary flow. it explains the antibacterial resistance of these matrix-enclosed sessile populations of bacteria. the catheter encrustation can be ...200111148750
properties of multidrug-resistant, esbl-producing proteus mirabilis isolates and possible role of beta-lactam/beta-lactamase inhibitor combinations.at our institution, isolation rates of clinical strains of esbl-producing proteus mirabilis increased to 8.8% of all p. mirabilis isolates during the period 1997-1999. to evaluate the susceptibility of esbl-producing p. mirabilis strains against commonly used drugs, we studied 50 non-duplicated isolates selected on the basis of synergy between clavulanate and beta-lactams (ceftazidime, aztreonam, cefotaxime, and ceftriaxone). the presence of esbl-coding genes was confirmed by colony hybridizatio ...200111165117
acute mastoiditis--the antibiotic era: a multicenter study.to evaluate the clinical course and identify the causative organisms of acute mastoiditis in a community where most of the patients who develop acute otitis media are treated with antibiotics.200111165635
urinary tract pathogens in complicated infection and in elderly individuals. 200111171003
resistance patterns among nosocomial pathogens: trends over the past few years.multiple surveillance studies have demonstrated that resistance among prevalent pathogens is increasing at an alarming rate, leading to greater patient morbidity and mortality from nosocomial infections. among gram-positive organisms, the most important resistant pathogens are methicillin- (oxacillin-)resistant staphylococcus aureus, beta-lactam-resistant and multidrug-resistant pneumococci, and vancomycin-resistant enterococci. important causes of gram-negative resistance include extended-spect ...200111171776
in vitro and in vivo antimicrobial activity of topical ofloxacin and other ototopical agents.ototopical agents are extensively used for otitis externa (oe), acute otitis media identified by otorrhea in patients who have tympanostomy tubes (aom-tt) and chronic suppurative otitis media (csom). the quinolones have particular value as ototopical agents because of the broad spectrum of antibacterial activity of importance in otic diseases and the high concentrations of antibacterial activity at the site of infection.200111176586
hypertrophic toe in a 44-year-old man. 200111176696
in vitro and in vivo properties of ro 63-9141, a novel broad-spectrum cephalosporin with activity against methicillin-resistant staphylococci.ro 63-9141 is a new member of the pyrrolidinone-3-ylidenemethyl cephem series of cephalosporins. its antibacterial spectrum was evaluated against significant gram-positive and gram-negative pathogens in comparison with those of reference drugs, including cefotaxime, cefepime, meropenem, and ciprofloxacin. ro 63-9141 showed high antibacterial in vitro activity against gram-positive bacteria except ampicillin-resistant enterococci, particularly vancomycin-resistant strains of enterococcus faecium. ...200111181368
[efficacy of bacteriophages in complex treatment of patients with burn wounds].results of clinical and laboratory evaluation of the treatment with pyobacteriophage in tablets of the patients with burn wounds are presented. it was shown that phagotherapy provided more rapid cure of pyoseptic complications, temperature normalization, wounds purification and lower lethality bacteriological analysis of wound secretions revealed that after the treatment staphylococci and streptococci were cultured 2 times rarely, proteus spp. were isolated 1.5 times rarely, e. coli was not isol ...200111221078
physiological and behavioral responses of stonefly nymphs to enhanced limestone treatment of acid mine drainage.a new acid mine drainage (amd) treatment system uses pulsed, fluidized beds of limestone, and carbon dioxide pretreatment of influent amd, to enhance limestone neutralization of amd. we conducted laboratory studies to evaluate the behavior and physiology of larval stoneflies (pteronarcys proteus, plecoptera) exposed to effluents produced by the treatment system. survival, sodium balance, drift, and feeding responses by p. proteus to treated and untreated amd were examined. p. proteus nymphs exhi ...200111228958
effect of subclinical uterine infection on cervical and uterine involution, estrous activity and fertility in postpartum buffaloes.nili-ravi buffaloes (n=29) that calved normally between august and november and did not develop any clinical reproductive disorder after calving were studied for the incidence of sub-clinical bacterial infection of the uterus and its effects on postpartum reproductive efficiency. the incidence of subclinical uterine infection was 24% (7/29). involution of the cervix and uterus was slower (p < 0.01) in the infected group than in the normal group (45.6 vs 31.1 days and 46.3 vs 35.8 days), respecti ...200111233783
[endonuclease from proteus mirabilis].two isoforms of nuclease displaying dnase and rnase activities were found in the culture liquid and periplasm of proteus mirabilis. the enzyme was isolated from the periplasm and then purified to a functionally homogeneous state. the nuclease was equally potent in cleaving denatured and native dnas by the endonuclease mechanism and was designated pm endonuclease. the endonuclease was shown to be a temperature-dependent enzyme with a ph optimum of 10.4-10.6, requiring the presence of bivalent met ...200111234403
immunocytochemical demonstration of visual pigments in the degenerate retinal and pineal photoreceptors of the blind cave salamander (proteus anguinus).visual pigments in the regressed eye and pineal of the depigmented neotenic urodele, the blind cave salamander (proteus anguinus anguinus), were studied by immunocytochemistry with anti-opsin antibodies. the study included light- and electron-microscopic investigations of both the eye and the pineal organ. a comparison was made with the black pigmented subspecies proteus anguinus parkelj (black proteus), which has a normal eye structure. in the retina of the black proteus, we found principal rod ...200111236001
bacterial protease ecp32 specifically hydrolyzing actin and its effect on cytoskeleton in vivo.a procedure for isolation of bacterial protease ecp32 yielding 100 microg of the enzyme from 10 liters of the escherichia coli strain a2 liquid culture has been developed. the procedure includes chromatography, ultrafiltration, and page under non-denaturing conditions. the purified preparation contained about 80% ecp32 and did not exhibit atpase activity. polyclonal ecp32-specific antibodies have been produced, and a two-stage procedure for the isolation of protease ecp32 involving affinity chro ...200111240398
lactobacillus plantarum reduces infection of pancreatic necrosis in experimental acute pancreatitis.infection is the commonest cause of death in acute pancreatitis. early reduction of commensal flora (particularly lactobacillus species) and, at the same time, overgrowth of enterobacteriaceae, especially escherichia coli, have recently been described during acute pancreatitis. lactobacillus plantarum has been shown to be effective in reducing the egress of endotoxin and microbial translocation in several experimental models such as chemically induced hepatitis and ulcerative colitis.200111244259
chronic osteomyelitis: clarification of nuclear medicine findings by fusion scans. 200111245118
investigation on the nutritive value and microbiological quality of wild quail carcasses.quail meats have many advantages and superiority one the other species of poultry. this study was planned to throw plenty of light on gross chemical composition, lipid fractions, fatty acids composition, amino acids composition, of thigh and breast of male and female wild quail meat as well as the microbiological quality. the mean values of moisture, protein, fat, ash and energy contents ranged from 60.1 to 69.2%, 55.0 to 68.8%, 28.8 to 42.1%, 2.40 to 3.63% and 696 to 1000 kj, respectively. seve ...200111253642
new tem variant (tem-92) produced by proteus mirabilis and providencia stuartii isolates.the sequences of the bla(tem) genes encoding tem-92 in proteus mirabilis and providencia stuartii isolates were determined and were found to be identical. except for positions 218 (lys-6) and 512 (lys-104), the nucleotide sequence of bla(tem-92) was identical to that of bla(tem-20), including the sequence of the promoter region harboring a 135-bp deletion combined with a g-162-->t substitution. the deduced amino acid sequence of tem-92 differed from that of tem-52 by the presence of a substituti ...200111257046
epigenetic regulation of carnitine metabolising enzymes in proteus sp. under aerobic conditions.proteus sp. is able to catalyse the reversible transformation of crotonobetaine into l(-)-carnitine during aerobic growth. contrary to other enterobacteriaceae no reduction of crotonobetaine into gamma-butyrobetaine could be detected in the culture supernatants. activities of l(-)-carnitine dehydratase, carnitine racemasing system and crotonobetaine reductase could be determined enzymatically in cell-free extracts of proteus sp. small amounts of gamma-butyrobetaine were found in cell-free extrac ...200111257539
interaction of bladder glycoprotein gp51 with uropathogenic bacteria.a major component of bladder surface mucin is a glycoprotein gp51 (molecular weight 51 kd.). gp51, which has previously been isolated from rabbit mucosa, appears to function as part of the defense mechanism in an in vivo infection model. gp51 coats the epithelium and is secreted into the urine, as detected by immunohistochemical testing and enzyme-linked immunosorbent assay (elisa). increased urinary gp51 occurs during urinary tract infection. to elucidate the role of gp51 as a component of the ...200111257713
comparison of the pr mutant with the wild-type strain of proteus mirabilis brings insight into peroxide resistance factors and regulation of catalase expression.the peroxide resistant mutant (pr) of proteus mirabilis was characterized by an increased constitutive catalase activity concomitant with a large production of specific mrna. survival toward hydrogen peroxide during exponential phase was increased by h2o2 pretreatment in the wild type but not in the mutant, although the catalase of both strains was not inducible under these conditions. in the mutant, besides catalase, over-produced proteins comprised two different alkyl hydroperoxide reductase s ...200111261492
differentiation of bacterial autolysins by zymogram analysis.the use of zymograms in which the bacterial cell wall heteropolymer peptidoglycan is incorporated into the resolving gel of sds-page has led to the identification of various sds stable peptidoglycan hydrolases (autolysins). to examine the specificity of autolysins with respect to o-acetylated peptidoglycan, a discontinuous sds-page system has been developed that operates under neutral conditions. [bis(2-hydroxyethyl)imino]tris(hydroxymethyl)methane (bis-tris) buffers are employed with ph 6.8 and ...200111262168
antimicrobial activity of honokiol and magnolol isolated from magnolia officinalis.the antimicrobial activity of honokiol and magnolol, the main constituents of magnolia officinalis was investigated. the antimicrobial activity was assayed by the agar dilution method using brain heart infusion medium and the minimum inhibitory concentration (mic) were determined for each compound using a twofold serial dilution assay. the results showed that honokiol and magnolol have a marked antimicrobial effect (mic = 25 microg/ml) against actinobacillus actinomycetemcomitans, porphyromonas ...200111268114
synthesis of mannich bases of some 2,5-disubstituted 4-thiazolidinones and evaluation of their antimicrobial activities.5-phenyl/methyl-5-morpholinomethyl/pyrrolidinomethyl-2-(5- aryl-1,3,4-oxadiazol-2-yl)imino]-4-thiazolidinones (5a-m) were synthesized by the reaction of 5-phenyl/methyl-2-[(5-aryl-1,3,4-oxadiazol -2-yl)imino]-4-thiazolidinones (4a-j) with formaldehyde and morpholine or pyrrolidine. the structures of the compounds were determined by analytical and spectral (ir, 1h-nmr, eims) methods. the antibacterial activities of the novel compounds against staphylococcus aureus atcc 6538, staphylococcus epider ...200111268772
the structure of the carbohydrate backbone of the core-lipid a region of the lipopolysaccharide from proteus penneri strain 40: new proteus strains containing open-chain acetal-linked n-acetylgalactosamine in the core part of the lps.analysis of the core part of the lps from several strains of proteus revealed that p. penneri strains 2, 11, 19, 107, and p. vulgaris serotypes 04 and 08 have the same structure with a new type of linkage between monosaccharidesan open-chain acetal--that was previously determined for p. vulgaris ox2 and p. penneri 17. the lps from p. penneri strain 40 contains the same structure substituted with one additional monosaccharide: [molecular structure: see text] where (1s)-galanac1 is a residue of n- ...200111269407
biodiversity and horizontal gene transfer in culturable bacteria isolated from activated sludge enriched in nonylphenol ethoxylates.one hundred and twenty bacterial isolates, from activated sludge of a treatment plant collecting wastes enriched in ethoxylated nonylphenols, were studied. sixty isolates were selected on rich medium and 60 on mineral medium containing two nonylphenol ethoxylates as the sole carbon source. analysis of biodiversity at the species level was performed by comparing the alui restriction patterns of the 16s ribosomal dna amplified by pcr from 120 isolates. the rdna restriction analysis enabled us to c ...200111281319
biochemical identification and characterization of dna groups within the proteus vulgaris complex.we placed 43 isolates belonging to the proteus vulgaris complex into proposed dna groups (genomovars) using five previously recommended tests (tests for esculin hydrolysis, production of acid from salicin, l-rhamnose fermentation, and elaboration of dnase and lipase). on the basis of the results of these five tests, 49% of the isolates fell into dna groups 5 and 6, 37% fell into dna group 2, and the remaining 14% fell into dna groups 3 and 4. sequencing of the 16s rrna genes of 11 members of dna ...200111283033
xanthogranulomatous pyelonephritis in childhood.demographic data, clinical presentation, associated abnormalities, and radiologic findings were evaluated to outline diagnostic criteria that may lead to the diagnosis of xanthogranulomatous pyelonephritis in children.200111283885
intradermal skin test with diabetes specific antigens in patients with type 1 diabetes.cell mediated immune response in vitro to a number of antigens has been reported in patients with type 1 diabetes. the aim of the present study was to develop an in vivo intradermal (delayed type hypersensitivity) skin test using antigens known to be recognized by lymphocytes of patients with type 1 diabetes and to compare, where possible, the in vivo response to the in vitro t cell proliferation to the same antigens. the skin test was performed in the following group of patients: 55 with recent ...200111298123
proteus syndrome: a variant with eye involvement. 200111318277
[new pyrrolo-pyrimidine derivatives with antifungal or antibacterial properties in vitro]. 200111320339
variations in the prevalence of strains expressing an extended-spectrum beta-lactamase phenotype and characterization of isolates from europe, the americas, and the western pacific region.to evaluate the prevalence of extended-spectrum beta-lactamase (esbl)-producing strains among species of enterobacteriaceae, a microdilution susceptibility test was performed with strains of klebsiella pneumoniae, escherichia coli, proteus mirabilis, and salmonella species that were isolated as part of the sentry project. the highest percentage of esbl phenotype (defined as a minimum inhibitory concentration [mic] > or =2 microg/ml for ceftazidime, ceftriaxone, or aztreonam) was detected among k ...200111320450
structure of the o-specific polysaccharide of proteus vulgaris o4 containing a new component of bacterial polysaccharides, 4,6-dideoxy-4.a high-molecular-mass o-specific polysaccharide was obtained by mild acid degradation of proteus vulgaris o4 lipopolysaccharide followed by gpc. the polysaccharide was studied by chemical methods along with 1h and 13c nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, h-detected 1h,13c hmqc, and 1h,13c hmbc experiments. solvolysis of the polysaccharide with trifluoromethanesulfonic (triflic) acid resulted in a glcpa-(1 --> 3)-glcnac disaccharide and a novel amino sugar derivative, 4 ...200111322733
structure of the o-specific polysaccharide of proteus mirabilis o16 containing ethanolamine phosphate and ribitol phosphate.the o-specific polysaccharide of proteus mirabilis o16 was studied by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,13c hmqc, hmqc-tocsy, and 1h,31p hmqc experiments, along with chemical methods. the polysaccharide was found to be a ribitol teichoic acid-like polymer having the following structure [structure: see text].200111322736
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