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polymerase chain reaction for the rapid diagnosis of tuberculous meningitis.(i)200214510337
[ study of exo-enzymatic profile and cytotoxic effect of bacterial culture supernatants in different growth phase].bacterial quorum-sensing represents an ubiquitary regulating system in which the pheromones (small molecules with different chemical structures, i.e. homoserin-lactones, octapeptides, aminoacids) act as extracellular mediators of signaling and intercellular communication. this chemical system is implicated in the regulation of different physiological processes dependent on the cell density (i.e. biolumniscence, virulence factors expression, sporulation, conjugation, antibiotic secretion etc). it ...200215085600
antibacterial studies of cefixime copper, zinc and cadmium complexes.antibacterial studies of metal complexes of cefixime, with copper zinc and cadmium have been carried out by observing the minimum inhibitory concentration (mic) and by measuring the zone of inhibition of the complexes and compared with the parent cephalosporin against both gram negative and gram positive microorganisms. various microorganisms used were staphylococcus aureus, e. coli, klebsiella and proteus species. for mic observation, serial dilution method was employed and zone sizes were dete ...200216414863
clarithromycin synergism with essential and trace elements.in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various macrolide antibiotics, the synergistic or antagonistic behavior of clarithromycin metal complexes has been studied and compared with the parent drug. metal complexes of clarithromycin with magnesium, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc and cadmium has been investigated for their antibacterial activity and compared with clarithromycin by observin ...200216414867
asymptomatic bacteriuria among outpatients with diabetes mellitus in an urban black population.to determine the prevalence of asymptomatic bacteriuria (asb) in individuals afflicted by diabetes mellitus; the antibiotic susceptibility of the microbial isolates and the association of host factors with asb.200214562524
antibiotic sensitivity pattern of the bacteria isolated from nosocomial infections in icu.the incidence of nosocomial infections in icu is 4-5 times greater than in general ward. critically ill patients are always at higher risk of developing nosocomial infections with resistant strains. this study is an attempt to know the antibiotic sensitivity pattern of the common isolates in icu. samples comprising urine, blood, endotracheal secretions and throat swabs were collected from 102 icu patients of them, 56 patients showed evidence of nosocomial infection (54.9%), from whom 120 differe ...200214710856
unilateral generalized hypertrichosis in proteus syndrome.a young lady was found to have hemihypertrophy, scoliosis, subcutaneous swellings, patchy dermal hypoplasia, plantar hyperplasia and macrodactyly. these suggested a diagnosis of proteus syndrome. she was also having unilateral generalized hypertrichosis. the association of proteus syndrome and unilateral generalized hypertrichosis has not been reported in the literature.200217656984
antibiotic resistance pattern in uropathogens.uropathogenic strains from inpatient and outpatient departments were studied from april 1997 to march 1999 for their susceptibility profiles. the various isolates were escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, proteus mirabilis, acinetobacter baumanii and enterococcus faecalis. antibiotic susceptibility pattern of these isolates revealed that for outpatients, first generation cephalosporins, nitrofurantoin, norfloxacin/ciprofloxacin were effective for treatment of urinary ...200217657041
haemagglutination, haemolysin production and serum resistance of proteus and related species isolated from clinical sources.a total of 148 strains of proteus and related species comprising of proteus mirabilis (116), proteus vulgaris (24), providentia rettgeri (4), providentia alcalifaciens (2), providentia stuarti (1) and morganella morganii (1), isolated from various sources, were examined for haemagglutination (ha), haemolysin production (hl) and serum resistance (sr). maximum isolates were obtained from urine (47.30%) and pus (40.54%) and they were multidrug resistant. the sensitivity to ciprofloxacin was 78.38%, ...200117664798
susceptibility of community gram-negative urinary tract isolates to mecillinam and other oral agents.to determine the susceptibility of community outpatient gram-negative urinary tract isolates to mecillinam and other commonly used oral agents.200118159352
comparative in-vitro activities of commonly available quinolones and other antibiotics on bacterial isolates in ibadan, nigeria.the 4-quinolones, many of which are now available in nigeria under different trade names, have a broad spectrum of activity. an evaluation is made of the comparative in-vitro activities of these quinolones and other antibiotics against 125 strains of bacteria commonly isolated from clinical specimens in university college hospital, ibadan, by using the stroke's disc sensitivity method, and mic estimation. the quinolones showed greater activity than the cephalosprins against klebsiella spp., prot ...200114510147
urinary calculi: bacteriological and chemical association.we investigated the bacteriology of urinary calculi in relation to urinary tract infection, stone formation, chemical composition and antibiotic sensitivity. fifty-two patients (37 males, 15 females) with urolithiasis were studied. urine, serum and urinary calculi specimens were taken and serum biochemical tests to detect uric acid, calcium and phosphorus were performed. urine analysis and culture were also performed. of the 52 patients, 19 (37%) had associated urinary tract infection, with esch ...200115332777
[bacterial causes of meningitis in newborns].bacterial meningitis jeopardizes the life of affected newborns and in survived children often leaves permanent sequels. the sooner the diagnosis is established and the therapy is started, the more a prosperous outcome can be expected. when the indication is established, lumbar punction should be performed. on the basis of the results of cytological and biochemical analyses of the cerebrospinal fluid (csf) that point to bacterial meningitis, the initial therapy should begin even before the result ...200115637989
directed evolution and phenomics screening of new biocatalysts.biodiversity screening and directed evolution are two fruitful complementary approaches for the discovery and design of novel biocatalysts. a new technology for directed evolution, l-shuffling, has been designed and patented by proteus. l-shuffling technology offers several competitive advantages over other technologies including (i) directed evolution of large genes: l-shuffling" means "large-shuffling"; (ii) high fidelity recombination and (iii) control over location and frequency of recombina ...200115954596
[recurrent purulent bacterial meningoencephalitis].during the period of 25 years there were 55 patients treated in our institute because of recurrent purulent bacterial meningoencephalitis(rpbme). this group consisted of 42 males (76%) and 13 (24%) females, the prevalent number (53%) of patients being under 21 years of age. the diagnosis of rpbme was based on the commonly accepted criteria and confirmed by the laboratory results of csf examination. the cause of the recurrences was established considering the skull x-ray examination, ct and mri. ...200111921730
penile prosthesis infections.in this retrospective review we looked at infection rates and risk factors in penile prosthesis recipients. computer registry records of patients implanted with three-piece inflatable prostheses from april 1986 to march 1999 were reviewed. bacteriological culture report data were obtained from the medical records. there were 491 three-piece inflatable penile prosthesis recipients with follow-up ranging from 1 to 168 months (mean 83). all patients were implanted more than 1 y ago. there were 10 i ...200111918248
antibiotic susceptibility of blood culture isolates of enterobacteriaceae. a norwegian multicenter study.from may to november 1997 each of six major hospitals throughout norway collected 72 to 104 consecutive blood culture isolates of enterobacteriaceae, altogether 563 isolates. escherichia coli was the predominating organism (69%), followed by klebsiella spp. (15%), enterobacter spp. (6%), and proteus mirabilis (4%). the susceptibility of the isolates to ampicillin, cefuroxime, ceftazidime, imipenem, tobramycin, and ciprofloxacin was determined by the e-test. 37% and 7% of the isolates were resist ...200111890566
comparison of aztreonam against other antibiotics used in urinary tract infections.urinary tract infection is a very common problem in adults as well as in children. there is always need for the right antibiotic to be chosen for treatment.200111873424
in vitro activity of moxifloxacin against local bacterial isolates.the emergence of resistance to common antimicrobials in bacteria has been increasingly reported in various countries. empirical antimicrobial therapy of various infections would therefore need to be reviewed. the introduction of new fluoroquinolones has created an interest in the use of these as possible agents in the empirical treatment of respiratory tract infections.200111817288
clinical and bacteriological aspects on the use of oxytetracycline and flunixin in primiparous cows with induced retained placenta and post-partal endometritis.retention of the fetal membranes and post-partal endometritis (rfm) are common problems in dairy cows. treatment often includes manual removal of the placenta in combination with antibiotic treatment. earlier studies have shown that cows with endometritis post-partum have a strong tendency to recover spontaneously. the present study focused on treatments of post-partal endometritis with the prostaglandin synthesis inhibitor, flunixin (f) either alone or combined with oxytetracycline (t). the stu ...200111885742
[etiological factors of urinary tract infections in children].etiological factors of urinary tract infection in children have been studied. the escherichia coli, klebsiella sp., enterococcus sp. and enterobacter sp. were the most often isolated bacteria from infant and baby urine. in this age group of patients uromycoses were also affirmed. from the urine of older children (1-18 years old) mainly escherichia coli, enterococcus sp., proteus sp. and morganella morganii have been raised. uropathogens' occurrence was analysed by a statistical method in the rel ...200111852813
synthesis and antimicrobial activity of some 1,2,4-triazole-3-mercaptoacetic acid derivatives.ethyl 5-(2-furyl)-4-ethyl-1,2,4-triazole-3-mercaptoacetate (2), 5-(2-furyl)-4-ethyl-1,2,4-triazole-3-mercaptoacetic acid hydrazide (3) and a series of new n-alkylidene/arylidene-5-(2-furyl)-4-ethyl-1,2,4-triazole-3-mercaptoacetic acid hydrazides (4a-f) were synthesized and evaluated for in vitro antibacterial activity against staphylococcus aureus atcc 6538. staphylococcus epidermidis atcc 12228, klebsiella pneumoniae atcc 4352, pseudomonas aeruginosa atcc 1539, escherichia coli atcc 8739, shige ...200111829115
synthesis and evaluation of antibacterial activity of some 2-[[alpha-(4-substituted benzoyloxy)-alpha-phenylacetyl or methylacetyl]amino]-5-(4-methoxyphenyl)- 1,3,4-oxadiazoles.in this study, a new series of 1-[[alpha-(4-substitutedbenzoyloxy)-alpha-phenylacetyl or methylacetyl]amino]-5-(4-methoxyphenyl)-1,3,4-oxadiazoles were obtained by condensation of 2-[(alpha-chloro-alpha-phenylacetyl or alpha-bromopropionyl)amino]-5-(4-methoxyphenyl)1,3,4-oxadiazoles with sodium salts of 4-substituted benzoic acids. structures of the compounds were assigned on the basis of spectral data (uv, ir, 1h nmr, el ms) and elemental analyses. the antibacterial activities of the novel comp ...200111829119
a new biologically active triterpenoid saponin from the leaves of lepidagathis hyalina nees.a new biologically active triterpenoid saponin m.f. c42h68o13, m.p. 315 degrees-317 degrees c was isolated from the ethyl acetate soluble fraction of the methanolic extract of the leaves of lepidagathis hyalina. its structure was characterized as 3-beta-o-[alpha-l-rhamnopyranosyl(1-->4)o-beta-d-glucopyranosyl]16-alpha-hydroxy-olean-12-en(13)-28-oic acid by several spectral and chemical analysis. this new triterpenoid saponin showed antimicrobial activity against various plants pathogenic bacteri ...200111841115
a novel germline mutation of the pten gene in a patient with macrocephaly, ventricular dilatation, and features of vater association.mutations of the pten gene are associated with hamartoma-neoplasia syndromes. while germline mutations at this chromosome 10q22-23 locus have been observed in patients with cowden syndrome (cs) and bannayan-riley-ruvalcaba syndrome (brr), both of which phenotypes are associated with hamartomata and neoplasia, somatic mutation of pten has been established in a wide variety of sporadically occurring neoplasia. cs and brr share some clinical features, specifically hamartomata and lipomatosis. inves ...200111748304
minipodia and rosette contacts are adhesive organelles present in free-living amoebae.using scanning electron microscopy, amoeba proteus cells migrating on the glass have been shown to develop dense coats of minipodia, which are discrete microprotrusions up to 8 microm long and approximately 0.5 microm across. they cover the middle-anterior area of the ventral cell surface, i.e. the region previously determined as the zone of most efficient adhesion of an amoeba to its substratum. minipodia are sparse underneath the frontal zone and lacking from the tail region. in amoebae that a ...200111748921
catalytic and structural properties of irt-21 beta-lactamase (tem-77) from a co-amoxiclav-resistant proteus mirabilis isolate.proteus mirabilis strain mag1, a clinical isolate that is resistant to broad-spectrum penicillins and co-amoxiclav, produces inhibitor-resistant tem (irt)-21, a novel mutant of tem beta-lactamase. this enzyme has a pi of 5.2 and is derived from the bla(tem-1a) gene ancestor. it contains two major amino acid substitutions specific for co-amoxiclav resistance (leu-69 for met and ser-244 for arg) that have never been found together previously. the dramatic loss of sensitivity to clavulanic acid, th ...200111750800
[tests for studying invasive properties of selected proteus mirabilis strains].proteus mirabilis is an important pathogen of the urinary tract infections (uti). lipopolysaccharide (lps, endotoxin) is one of the pathogenic factors of pathogenicity of these bacteria. in this paper we described the invasion of l929 mouse fibroblasts by p. mirabilis strains, classified into the o10, o23, o30, o43 serogroups. the maximal invasiveness was observed between 4-6 hours of incubation of the tested cells with bacteria. the cytotoxic effect slightly increased with the incubation time, ...200111785185
molecular properties and metabolic effect on blood cells produced by a new toxin of enterobacter cloacae.a new toxin of enterobacter cloacae able to lyse erythrocytes and leukocytes was found. purification of the toxin was performed by salt precipitation, gel filtration, ion exchange and hplc in c8 column. sds-page electrophoresis showed more than one bank corresponding to the leukotoxin able to form polymers and aggregate like some pore-forming cytotoxins (rtx). in culture supernatant the toxin showed 1 hu/ml (hemolytic unit) and 1.5 lu/ml (leukotoxic unit); after purification it reached 15 hu/ml ...200111787862
[infective lithiasis. treatment and complications].to establish the criteria for treatment of staghorn stones according to stone surface and distribution in cadaver kidneys.200111789372
adhesion of thrombin-stimulated and unstimulated blood platelets to collagen in the presence of proteus mirabilis lipopolysaccharides.adhesion of blood platelets to collagen may be involved in pathogenesis of septic shock after damage of endothelial cells by lps or inflammatory cytokines. therefore, analysis of platelet adhesion in the presence of endotoxin seems to be of great importance. we studied in vitro the effects of lpss (s1959, r110, r45) from proteus mirabilis (smooth and rough types differing significantly in their composition) on the adhesion of unstimulated and thrombin-stimulated platelets to collagen. the adhesi ...200111798396
synthesis and antibacterial activity of 5-nitrofuryl and 3-methoxy-2-nitrophenyl derivatives of 6 beta-aminopenicillanic, 7 beta-aminocephalosporanic and 7 beta-aminodesacetoxy-cephalosporanic acids.a number of 5-nitrofuryl and 3-methoxy-2-nitrophenyl derivatives of 6 beta-aminopenicillanic (6 beta-apa), 7 beta-aminocephalosporanic (7 beta-aca) and 7 beta-aminodesacetoxycephalosporanic (7 beta-adca) acids were synthesized by the method of mixed anhydrides or via schiff bases. the chemical structures of the new compounds were confirmed by ir-, 1h-nmr and mass spectral data, obtained by negative ion electrospray ionization. the in vitro testing results indicated that all penicillins and cepha ...200111799847
galectin-3-mediated adherence of proteus mirabilis to madin-darby canine kidney cells.proteus mirabilis is an important cause of urinary tract infections (utis) and can result in acute pyelonephritis. proteus mirabilis expresses several, morphologically distinct, fimbrial species, and previous studies have shown that the nonagglutinating fimbriae (naf) mediate bacterial adherence to a number of cell lines, including madin-darby canine kidney (mdck) cells. immunoblot overlay analysis of the plasma membrane fraction from mdck cells with purified naf revealed a 34-kda band, which ha ...200111800020
influence of different natural physical fields on biological processes.in space flight conditions gravity, magnetic, and electrical fields as well as ionizing radiation change both in size, and in direction. this causes disruptions in the conduct of some physical processes, chemical reactions, and metabolism in living organisms. in these conditions organisms of different phylogenetic level change their metabolic reactions undergo changes such as disturbances in ionic exchange both in lower and in higher plants, changes in cell morphology for example, gyrosity in pr ...200111803963
sudden increase in pseudomonas aeruginosa nosocomial strains with broad host range transfer of antibiotic resistance.we investigated transfer of antibiotic resistance from 51 multiply resistant strains of pseudomonas aeruginosa isolated from seriously ill patients in the frankfurt university clinics. nine isolates directly transferred resistance to three recipient strains used. ticarcillin and cephalothin resistance determinants were accepted from eight isolates, and in one case a kanamycin resistance determinant was transferred. the total spectrum of resistance transferred demonstrated that several donor stra ...200111806620
pten mutations and proteus syndrome. 200111755638
pten mutations and proteus syndrome. 200111755639
cerebriform fibrous proliferation vs. proteus syndrome.proteus syndrome is a rare, congenital hamartomatous syndrome that presents with a wide range of abnormalities. regardless of different manifestations found in different patients, there exists three mandatory criteria for the diagnosis of this syndrome: a mosaic distribution of the lesions, a progressive course, and sporadic occurrence. when these criteria are met, the presence of additional connective tissue nevi, which are encountered mostly on the plantar surface of the feet, suffices for the ...200111756840
characterisation of p-nitrophenylglycerol-resistant proteus mirabilis super-swarming mutants.p-nitrophenylglycerol (pnpg) inhibits the co-ordinately regulated activities of swarming behaviour and virulence factor expression in proteus mirabilis. the inhibitory action of pnpg was investigated by the isolation of tn5 insertion mutants that could swarm, albeit with much reduced ability, in the presence of pnpg. the mutants exhibited a super-swarming phenotype in the absence of pnpg; i.e., they migrated further in a given time than did the wild-type cells. cloning and sequence analysis of t ...200111761187
synthesis and antimicrobial activity of new diazoimidazole derivatives containing an n-acylpyrrolidine ring.a series of 4-diazoimidazole-5-carboxamides bearing in position 2 lipophilic substituents was synthesized and their antimicrobial activity was evaluated in vitro against pathogenic gram-positive, gram-negative bacteria and fungi. some compounds presented antifungal activity, particularly two derivatives (1g and 1h) showed good mic values (10-50 microg/ml) against both moulds and yeasts.200111765041
bacterial agents of discharging ears and antimicrobial sensitivity patterns in children in ibadan, nigeria.bacteriological studies were carried out on ear swabs from 361 cases of children with discharging ears at the microbiology department of the university college hospital, ibadan between march 1995 and february 1997. 308 (85.3%) had positive cultures. 78.6% of these yielded one isolate, 19.2% yielded two isolates while 2.2% had three isolates. pseudomonas spp. was the predominant agent of csom and asom. this was followed by staphylococcus aureus. other commonly isolated organisms were proteus and ...200111768012
pyogenic thyroiditis and hiv infection.infective thyroiditis remain rare disease entity. six patients were seen in jos, nigeria in the last five years. all of them were hiv positive. the presentation and management of these patients are high-lighted. the clinical features and bacteriology were similar to those previously documented for infective thyroiditis.200111768021
normal oral bacterial flora from some southern african snakes.eighteen snakes representing 11 species were subject to mouth swabbing on 58 occasions. of these swabs, 52.2% were positive for bacteria. a total of 92 bacterial isolates were cultured, representing 30 species of which 81.5% were enterobacteriaceae, 16.3% gram positive cocci, and 2.2% anaerobes. swabs from non-venomous snakes were more commonly bacteriologically sterile than those from venomous snakes (p = 0.0107). the oral bacterial flora did not differ between captive and newly captured snakes ...200111769348
comparison of laterally condensed .06 and .02 tapered gutta-percha and sealer in vitro.the purpose of this in vitro study was to compare the quality of the seal in canals prepared in a standardized manner and obturated with a .06 or a .02 tapered gutta-percha master cone using lateral condensation. forty-four extracted human anterior teeth with single, straight canals were divided into two experimental groups of 20 teeth each and two control groups of 2 teeth each. the teeth were instrumented with series 29 profile .06 tapered rotary nickel-titanium files to a master apical file o ...200111771592
ertapenem: a new carbapenem.ertapenem is a new 1-beta-methyl carbapenem, stable to dehydropeptidase, which binds preferable to penicillin-binding proteins (pbp) 2 and 3. ertapenem has a broad antibacterial spectrum with mic90 values < 0.5 mg/l for penicillin-susceptible streptococcus pneumoniae, streptococcus pyogenes, methicillin-sensitive staphylococcus aureus, haemophilus influenzae, moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., serratia spp., proteus spp., clostridium perfringens, fusobact ...200111772242
mystic program: summary of european data from 1997 to 2000.the meropenem yearly susceptibility test information collection (mystic) program is a global study providing in-vitro surveillance data on microbial susceptibility in centers that prescribe meropenem. this paper summarizes data on the activity of meropenem and five comparator agents against 13,793 clinical isolates from 31 centers in 10 european countries, 1997-2000. meropenem and imipenem demonstrated broad-spectrum activity against the tested organisms, including beta-lactamase-producing strai ...200111777657
sonographic detection of multiple brain abscesses in a newborn with iga deficiency.we report the case of a neonate with selective iga deficiency and multiple brain abscesses diagnosed with sonography. brain sonography revealed multiple abscesses in the left hemisphere; the abscesses ranged from 10 to 20 mm. cultures obtained from the cerebrospinal fluid and blood were positive for proteus mirabilis. the neonate responded promptly to broad-spectrum antibiotic therapy and had no neurologic sequelae. because iga deficiency is associated with infections, we believe it was a predis ...200111745857
[number, activity and thermostability of the electrophoretic forms of acid phosphatase in amoeba proteus, cultured at different temperatures].in free-living amoebae (amoeba proteus, strain b), cultured at 10 and 25 degrees c, we compared the number, activity, and thermostability of separate electromorphs of triton-soluble acid phosphatase (acp) revealed by disc-electrophoresis in polyacrylamide gel using 2-naphthyl phosphate (ph 4.0) as a substrate. no differences in the number of acp electromorphs and their mobility were observed at both these temperatures. the total activity of acp electromorphas per unit of cellular protein and the ...200111534183
ssu rrna-based phylogenetic position of the genera amoeba and chaos (lobosea, gymnamoebia): the origin of gymnamoebae revisited.naked lobose amoebae (gymnamoebae) are among the most abundant group of protists present in all aquatic and terrestrial biotopes. yet, because of lack of informative morphological characters, the origin and evolutionary history of gymnamoebae are poorly known. the first molecular studies revealed multiple origins for the amoeboid lineages and an extraordinary diversity of amoebae species. molecular data, however, exist only for a few species of the numerous taxa belonging to this group. here, we ...200111719580
structure of the o-specific polysaccharide of proteus vulgaris o37 and close serological relatedness of the lipopolysaccharides of p. vulgaris o37 and p. vulgaris o46.the o-specific polysaccharide (o-antigen) of the lipopolysaccharide (lps) of proteus vulgaris o37 was studied by (1)h and (13)c nuclear magnetic resonance spectroscopy before and after o-deacetylation and found to be structurally similar to that of p. vulgaris o46 studied earlier. the two polysaccharides have the same carbohydrate backbone and differ in the position and number of the o-acetyl groups only. studies with o-antisera against the two strains using passive hemolysis test, enzyme immuno ...200111720819
purification and characterization of the groeslx chaperonins from the symbiotic x-bacteria in amoeba proteus.groelx and groesx proteins of symbiotic x-bacteria from amoeba proteus were overproduced in escherichia coli transformed with pajx91 and puxgprm, respectively, and their chaperonin functions were assayed. we utilized sigma(70)-dependent specific promoters of groex in the expression vectors and grew recombinant cells at 37 degrees c to minimize coexpression of host groe of e. coli. for purifying the proteins, we applied the principle of heat stability for groelx and pi difference for groesx to mi ...200111722184
gut-derived bone infection in the neonatal rat.the risk of osteomyelitis is increased in the premature and critically ill neonate. although potential sites of bacterial entry are present in many of these infants, the source of infection frequently cannot be established. this study was performed to assess the possible role of bacterial translocation from the intestine in the origin of bone infection using models of breast-fed and formula-fed rat pups. newborn sprague-dawley rats suckled either ad libitum by the dam (n = 30), or were fed a rat ...200111726738
chaotic behavior in the locomotion of amoeba proteus.the locomotion of amoeba proteus has been investigated by algorithms evaluating correlation dimension and lyapunov spectrum developed in the field of nonlinear science. it is presumed by these parameters whether the random behavior of the system is stochastic or deterministic. for the analysis of the nonlinear parameters, n-dimensional time-delayed vectors have been reconstructed from a time series of periphery and area of a. proteus images captured with a charge-coupled-device camera, which cha ...200111732198
bilateral septic arthritis of the knee joint in three children caused by unusual infectious agents. 200111737753
urinary excretion and bactericidal activities of gemifloxacin and ofloxacin after a single oral dose in healthy volunteers.in a randomized crossover study, 16 volunteers (8 men, 8 women) received single oral doses of 320 mg of gemifloxacin and 400 mg of ofloxacin on two separate occasions in the fasting state to assess the urinary excretion and urinary bactericidal titers (ubts) at intervals for up to 144 h. ofloxacin showed higher concentrations in urine compared with those of gemifloxacin. the median (range) cumulative excretion of gemifloxacin was 29.7% (8.4 to 48.7%) of the parent drug administered, and median ( ...200111709334
cefepime, piperacillin-tazobactam, and the inoculum effect in tests with extended-spectrum beta-lactamase-producing enterobacteriaceae.there is little information about the clinical effectiveness of cefepime and piperacillin-tazobactam in the treatment of infections caused by extended-spectrum beta-lactamase (esbl)-producing pathogens. some inferences have been drawn from laboratory studies, which have usually involved only one or a few strains of esbl-producing klebsiella pneumoniae or escherichia coli that produced only a limited range of esbls. such studies are indirect, sometimes conflicting, indicators of efficacy. to exte ...200111709338
tem-89 beta-lactamase produced by a proteus mirabilis clinical isolate: new complex mutant (cmt 3) with mutations in both tem-59 (irt-17) and tem-3.tem-89 (cmt-3) is the first complex mutant beta-lactamase produced by a clinical strain of proteus mirabilis (strain pm 631). this new enzyme, which has a pi of 6.28, is derived from tem-3 and has a single amino acid substitution also encountered in tem-59 (inhibitor-resistant tem beta-lactamase irt-17): ser-130 to gly. tem-89 hydrolyzed penicillins to the same extent that tem-3 did but lost almost all hydrolytic activity for cephalosporins and, like tem-59, was highly resistant to inhibitors.200111709345
proteus mirabilis septic monoarthritis in a patient with cpdd. 200111344817
structure and serological characterization of an nepsilon-[(r)-1-carboxyethyl]-l-lysine-containing o-chain of the lipopolysaccharide of proteus mirabilis o13.in this paper we present the structure and describe serological properties of the o-specific polysaccharide of proteus mirabilis o13 lipopolysaccharide, which contains a unique component: an amide of d-galacturonic acid (d-gala) with an unusual amino acid, nepsilon-[(r)-1-carboxyethyl]-l-lysine (alaninolysine, alalys). selective chemical degradations of either gala or alalys resulted in the loss of the serological reactivity of the polysaccharide with anti-o serum against p. mirabilis o13. neith ...200111348021
fluorogenic assay for penicillin g acylase activity.a simple, highly sensitive, and rapid assay for high-throughput screening of penicillin g acylase-producing bacteria is presented. the method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin g acylase. the present method is suitable for screening pure enzymes as well as various penicillin g acylases like those from escherichia coli, proteus rettgeri, and kluyvera citrophila in cell extracts. in addi ...200111355855
[phosphatase from proteus mirabilis].cell-free preparations of proteus mirabilis contained a phosphatase (ec 3.1.3.1), whose activity surpassed that of alkaline phosphatase from escherichia coli. phosphatase was also found in the culture liquid of p. mirabilis. the composition of proteins displaying enzyme activity was assayed by polyacrylamide gel electrophoresis. enzyme synthesis was studied at various stages of bacterial growth. biosynthesis of phosphatase in p. mirabilis (similarly to that in other bacteria) was shown to be ind ...200111357420
the effect of resveratrol on the platelet secretory process induced by endotoxin and thrombin.the effect of resveratrol (trans-3,4',5-trihydroxystilbene) on the release of adenine nucleotides and proteins from blood platelets activated by lipopolysaccharide (lps), from proteus mirabilis and by thrombin, were studied. thrombin stimulated the release of adenine nucleotides from dense granules and proteins from alpha-granules. the lps (0.3 microg/10(8) platelets, 5 min, 37 degrees c), like thrombin (2.5 u/10(8) platelets, 5 min, 37 degrees c) was found to cause a release of adenine nucleoti ...200111368092
epidemiology and frequency of resistance among pathogens causing urinary tract infections in 1,510 hospitalized patients: a report from the sentry antimicrobial surveillance program (north america).bacterial urinary tract infections (utis) are an important cause of septicemia resulting in high mortality rates, prolonged hospital stays and increased healthcare costs. periodic reviews of pathogen frequency and susceptibility patterns impact on appropriate antimicrobial usage, leading to more effective prescribing practices. as part of the sentry antimicrobial surveillance program (sentry, 1998), participants collected 50 consecutive uti pathogens from patients hospitalized in 31 medical cent ...200111502382
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1999). i. susceptibility distribution].the bacterial strains isolated from patients diagnosed as having urinary tract infections (utis) in 9 institutions in japan were supplied between the period of august 1999 to july 2000. then, the susceptibilities of them to many kinds of antimicrobial agents were investigated. the number of them were 499 strains. the breakdown of these strains was gram-positive bacteria as 31.3% and gram-negative bacteria as 68.7%. susceptibilities of these bacteria to antimicrobial agents were as follows; vanco ...200111510118
urinary tract infections in patients with spinal cord lesions: treatment and prevention.even though the mortality due to urinary tract complications has decreased dramatically during the last decades in individuals with spinal cord lesions (scl), urinary tract infections (uti) still cause significant morbidity in this population. complicated uti are caused by a much wider variety of organisms in individuals with scl than in the general population and are often polymicrobial. escherichia coli, pseudomonas spp., klebsiella spp., proteus spp., serratia spp., providencia spp., enteroco ...200111511022
prevalence of antimicrobial resistance among urinary tract pathogens isolated from female outpatients across the us in 1999.in the united states, trimethoprim-sulphamethoxazole (tmp-smx) is the recommended first-line treatment for uncomplicated urinary tract infections (utis) in females, in regions with resistance rates of <10-20%. unfortunately, current data on regional resistance is often not readily available to physicians and regional variability in resistance remains largely unknown. this report presents antimicrobial susceptibility data for tmp-smx and three other commonly tested antimicrobials organized by sta ...200111516934
comparative antimicrobial spectrum and activity of bms284756 (t-3811, a desfluoroquinolone) tested against 656 enterobacteriaceae, including preliminary in vitro susceptibility test comparisons and development.bms284756 (t-3811), a novel des-f(6)-quinolone, was evaluated using isolates of enterobacteriaceae from the sentry antimicrobial surveillance program tested by etest (ab biodisk, solna, sweden), reference broth microdilution and disk diffusion (5-microg) methods. ciprofloxacin, levofloxacin, gemifloxacin and gatifloxacin were also tested by broth microdilution as comparator antimicrobial agents within the same drug class. the 656 isolate collection included species from the genera citrobacter, e ...200111516937
bacterial modification of lps and resistance to antimicrobial peptides.antimicrobial peptides (aps) are ubiquitous in nature and are thought to kill micro-organisms by affecting membrane integrity. these positively charged peptides interact with negative charges in the lps of gram-negative bacteria. a common mechanism of resistance to ap killing is lps modification. these modifications include fatty acid additions, phosphoethanolamine (petn) addition to the core and lipid a regions, 4-amino-4-deoxy-l-arabinose (ara4n) addition to the core and lipid a regions, acety ...200111521084
site-directed mutagenesis and phylogenetic comparisons of the escherichia coli tus protein: dna-protein interactions alone can not account for tus activity.the tus protein of escherichia coli is capable of arresting dna replication in an orientation-dependent manner when bound to specific sequences in the bacterial chromosome called ter sites. arrest of dna replication has been postulated to occur either by a barrier mechanism, where tus acts as a physical block to replication fork progression, or through protein-protein interactions between tus and some component of the replication fork. a previous mutational analysis of tus suggested that the ami ...200111523786
[proteus syndrome]. 200111528880
protean infectious types and frequent association with neurosurgical procedures in adult serratia marcescens cns infections: report of two cases and review of the literature.serratia marcescens is a rare pathogen of adult central nervous system (cns) infection. we report on the clinical features and therapeutic outcomes of two adult patients with such infections. the clinical characteristics of 13 other reported adult cases are also included for analysis. the 15 cases were nine males and six females, aged 19-83 years, in whom, underlying post-neurosurgical states and ear operation were noted in 93% (14/15). fever and conscious disturbance were the most common clinic ...200111532558
repression of bacterial motility by a novel fimbrial gene product.proteus mirabilis is a common uropathogen in patients with long-term catheterization or with structural or functional abnormalities in the urinary tract. the mannose-resistant, proteus-like (mr/p) fimbriae and flagellum are among virulence factors of p.mirabilis that contribute to its colonization in a murine model of ascending urinary tract infection. mrpj, the last of nine genes of the mrp operon, encodes a 107 amino acid protein that contains a putative helix-turn-helix domain. using transcri ...200111532949
cmy-2-producing salmonella enterica, klebsiella pneumoniae, klebsiella oxytoca, proteus mirabilis and escherichia coli strains isolated in spain (october 1999-december 2000).cmy-2 plasmid-mediated ampc beta-lactamase (cmy-2) was detected in 21 isolates from two hospitals located in different geographical regions of spain between october 1999 and december 2000. the isolates comprised two salmonella enterica serovars (mikawasima and montevideo), 16 escherichia coli, one klebsiella pneumoniae, one klebsiella oxytoca and one proteus mirabilis. in addition to the expected resistance to beta-lactams, including extended-spectrum cephalosporins and cefoxitin, all isolates s ...200111533003
intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae.intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ...200111544246
identification and localisation of caldesmon-like protein in amoeba proteus. 200111544656
effects of blocking of endogenous rho family gtp-binding proteins on morphology, adhesion and locomotion of amoeba proteus. 200111544664
filamentous structures in amoeba proteus visualized in scanning microscope. 200111544682
[humoral immune response in patients with bronchopulmanory infections to immunotherapy].the content of iga, igm, igg and the level of specific antibodies in the blood serum of patients with bronchopulmonary diseases after a course of immunotherapy with polycomponent vaccine b[symbol: see text]-4 was studied. a rise in the concentration of igm due to the synthesis of specific antibodies to proteus vulgaris, escherichia coli and klebsiella pneumoniae was found to occur. the examination of sick children revealed that a high proportion of them (54%) showed a pronounced decrease in the ...200111548259
[experimental study of photodynamic effect on bacterial wound microflora].the in vitro study of the influence of photodynamic action (with the use of photosensitizer "photosens", laser and non-laser irradiation) on the strains of the main representatives purulent wound microflora was carried out. staphylococcus aureus, s. epidermidis, pseudomonas aeruginosa, escherichia coli, proteus mirabilis cultures have been isolated from wound secretions and identified. the photosensitizer was shown to produce no bactericidal effect by itself. irradiation with laser and non-laser ...200111548270
new aspects of the role of mr/p fimbriae in proteus mirabilis urinary tract infection.proteus mirabilis, a common cause of urinary tract infection (uti), produces a number of different fimbriae including mannose-resistant proteus-like fimbriae (mr/p). the precise role of different p. mirabilis fimbriae in ascending uti has not yet been elucidated. in this study, a clinical isolate of p. mirabilis and an isogenic mutant unable to express mr/p were tested using different experimental approaches. they were tested for their ability to cause infection in an ascending co-infection mode ...200111549418
[intestinal microbiocenosis in children with intestinal enzymopathy].141 children with different kinds of intestinal enzymopathy were examined; of these, 33 had celiac disease, 39--the syndrome of celiac disease, 12--congenital lactase deficiency and 57--the syndrome of disaccharidase insufficiency. in these patients a significant decrease in the average characteristics of the main protective flora and the growth of hemolytic and lactose-negative enterobacteria were established. in all groups of patients increased amounts of proteus were detected, which was indic ...200111550576
involvement of coenzyme a esters and two new enzymes, an enoyl-coa hydratase and a coa-transferase, in the hydration of crotonobetaine to l-carnitine by escherichia coli.two proteins (caib and caid) were found to catalyze the reversible biotransformation of crotonobetaine to l-carnitine in escherichia coli in the presence of a cosubstrate (e.g., gamma-butyrobetainyl-coa or crotonobetainyl-coa). caib (45 kda) and caid (27 kda) were purified in two steps to electrophoretic homogeneity from overexpression strains. caib was identified as crotonobetainyl-coa:carnitine coa-transferase by maldi-tof mass spectrometry and enzymatic assays. the enzyme exhibits high cosubs ...200111551212
[activity and thermal stability of acid phosphatase in homogenates of amoeba proteus, acclimated to various temperatures].activity and thermoresistance of acid phosphatase were determined in supernatant of amoeba proteus homogenates using 1-naphthyl phosphate (ph 4.0) and p-nitrophenyl phosphate (ph 5.5). although tartrate-resistant and tartrate-sensitive acid phosphatases hydrolyse both substrates, the former mainly hydrolyses p-nitrophenyl phosphate and the latter 1-naphthyl phosphate. a decrease in the activity of the total and tartrate-sensitive acid phosphatases, when using 1-naphthyl phosphate, and of the tot ...200111552654
identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa.the bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after d-galactosamine-induced liver injury, with and without pretreatment with lactobacillus plantarum dsm 9843, were studied in the rat. dominating representatives were identified to species level by 16s rdna sequencing and typed by randomly amplified polymorphic dna (rapd) and by restriction endonuclease analysis (rea) for strain definition. it was proven that bacterial strai ...200111552954
isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis.the aim of this study was to determine the occurrence and role of yeasts, enteric gram-negative rods and enterococcus species in root-filled teeth with chronic apical periodontitis, and the antimicrobial effect of iodine potassium iodide (iki) irrigation.200111556508
proteus syndrome: a case report.proteus syndrome is a rare genetic disorder, characterized by partial gigantism of the hands and/or feet, asymmetry of the limbs, plantar hyperplasia, multiple hamartomatous subcutaneous tumors, hyperostoses, and long bone overgrowth. a one day old thai male infant is reported with macrosomia, hemihypertrophy of the left side of the face and left leg, large feet, macrodactyly of toes, plantar hyperplasia, large subcutaneous mass with a violet-red surface over the left side of the chest wall and ...200111560226
bioactive compounds from iostephane heterophylla (asteraceae).the novel bisabolene sesquiterpenes 3-6, were isolated from iostephane heterophylla, using bioguided fractionation. the new compounds were determined to be (12r/12s)-12,13-epoxy-xanthorrhizols (3,4) and (12r/12s)-12,13-dihydro-12,13-dihydroxy-xanthorrizols (5,6) and their structures were characterized by analysis of spectroscopic data and by chemical correlation from xanthorrhizol (2). the stereochemistry at c-12 of 5 was deduced using the modified mosher experiment. some of the isolated compoun ...200111561451
ligand diffusion in the catalase from proteus mirabilis: a molecular dynamics study.the role of the channels and cavities present in the catalase from proteus mirabilis (pmc) was investigated using molecular dynamics (md) simulations. the reactant and products of the reaction, h(2)o(2) -->1/2 o(2) + h(2)o, catalyzed by the enzyme were allowed to diffuse to and from the active site. dynamic fluctuations in the structure are found necessary for the opening of the major channel, identified in the x-ray model, which allows access to the active site. this channel is the only pathway ...200111567083
scoliosis in proteus syndrome: case report.the case of patient with scoliosis based on a rare hamartomatous, proteus syndrome, is reported.200111568716
gram-negative bacterial toe web infection: a survey of 123 cases from the district of cagliari, italy.foot intertrigo is mostly caused by dermatophytes and yeasts, less frequently by gram-positive and gram-negative bacteria. nevertheless, the importance of polymicrobial infections and especially colonizations of pseudomonas aeruginosa can cause therapy problems in relation to antibiotic resistance and the risk of potentially lethal complications.200111568743
[clinical significance of microbiological monitoring of infective agents in an urological hospital in the selection of antibacterial therapy regimens].individual microbiological examination of each patient can improve significantly the response of urological patients to antibacterial therapy. 6000 cultures of opportunistic microorganisms isolated from patients with various infectious-inflammatory diseases were studied. most of them were gram-negative: p. aeruginosa, e. coli, enterobacter spp., proteus spp. of gram-positive most frequently occurred staphylococcus spp and enterococcus faecalis. the cultures showed different spectrum of the sensi ...200111569227
[formation of intestinal microflora in children during the first year of their life].in 525 young children the state of intestinal microbiocenosis was studied every month of the first year their life. the study revealed that the process of the microflora formation lasted throughout the first year of their life and was characterized by dysbiotic disturbances. during this period the aggravation of dysbiotic changes in the intestine of these children on months 3, 6-7 and 11-12 was of particular importance. the formation of stable dysbacteriosis led to a decrease in the immunologica ...200111569262
electrochemical biosensor array for the identification of microorganisms based on lectin-lipopolysaccharide recognition.rapid identification of bacterial strains remains a well-known problem in applied medicine and, for viable pathogens, is an important diagnostic goal. we have investigated an electrochemical biosensor array, in which transduction is based on respiratory cycle activity measurements, where the microorganism's native respiratory chain is interrupted with non-native external oxidants. the selective biochemical recognition agents employed in this study are lectins that, once immobilized, recognize an ...200111569815
bacterial swarming: a biochemical time-resolved ftir-atr study of proteus mirabilis swarm-cell differentiation.fourier transform infrared spectroscopy was applied to the study of the differentiation process undergone by proteus mirabilis. this bacterium exhibits a remarkable dimorphism, allowing the cells to migrate on a solid substratum in a concerted manner yielding characteristic ring patterns. we performed an in situ noninvasive analysis of biochemical events occurring as vegetative cells differentiate into elongated, multinucleate, nonseptate, and hyperflagellated swarm cells. the major findings ari ...200111570895
[monitoring of uropathogens and their susceptibility to antibiotics].samples of urine collected from patients with complicated urology infection and hospitalized to the moscow region research clinical institute in 1986, 1991, 1995 and 1999 were analysed. of 11,444 samples examined, bacteriuria was estimated in 7143 samples. 9786 strains (29 genus) of bacteria were isolated--56.9 per cent as mono culture and 43.1 per cent as associations. susceptibility to 21 antibiotic was determined by disk diffusion method for 1607 strains; beta-lactamase production was determi ...200111573319
[clinical significance of infection pathogen resistance in a urological clinic for selection of antibacterial therapy regimes for the treatment of complicated urinary infections].data on changes of etiological structure of the complicated urinary tract infections treated at the research institute of urology and hospital no. 47 at the 5-year period (1996-2000) are given. it was shown that the most important microorganisms were the following: enterobacter spp., e. coli, proteus spp., staphylococcus spp., enterococcus faecalis. the correlation between frequency of the microorganisms isolation and their susceptibility to antimicrobial agents at different periods was analysed ...200111573320
rheumatoid arthritis: the future is not yet now. 200111574984
rheumatoid arthritis as an autoimmune disease caused by proteus urinary tract infections: a proposal for a therapeutic protocol. 200111574986
enzymatic characterization of tem-63, a tem-type extended spectrum beta-lactamase expressed in three different genera of enterobacteriaceae from south africa.the extended-spectrum beta-lactamase, tem-63, was identified in three separate genera of south african isolates: proteus mirabilis, klebsiella pneumoniae, and escherichia coli. this paper describes identification of the gene in these isolates and compares relative rates of hyrolysis between tem-63 and other known ceftazidimases.200111576794
isolation and structure elucidation of a new antifungal and antibacterial antibiotic produced by streptomyces sp. 201.an antibacterial and antifungal antibiotic was isolated from the culture filtrate of streptomyces sp. 201, and its structure was determined as 2-methyl-heptyl isonicotinate by extensive use of nmr spectroscopy. the compound exhibited marked antimicrobial activity against bacillus subtilis, shigella sp., klebsiella sp., e. coli, proteus mirabilis, and the pathogenic fungi, fusarium moniliforme, f. semitectum, f. oxysporum, f. solani and rhizoctonia solani.200111577728
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