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vitreous penetration of orally administered gatifloxacin in humans.to investigate the penetration of gatifloxacin, a novel extended-spectrum fluoroquinolone antibiotic, into the vitreous humor after oral administration.200212545689
[antibacterial activities and pk/pd parameters of aminoglycosides against recent clinical isolates of gram-negative rods].we examined antibacterial activities and pk/pd parameters of six kinds of aminoglycosides against seven bacterial species of clinical isolates in 2001. aminoglycoseides examined were gentamicin (gm), dibekacin (dkb), tobramycin (tob), amikacin (amk), netilmicin (ntl), and isepamicin (isp), and bacterial isolates used were each 50 strains of escherichia coli, klebsiella pneumoniae, enterobacter cloacae, citrobacter freundii, proteus spp., serratia marcescens and pseudomonas aeruginosa. all aminog ...200212532635
[comparative population study of the etiological role of causative agents of purulent inflammatory diseases and serum antibodies to antigens of opportunistic microorganisms].the comparative study of the relationship between the levels of serum antibodies to the antigens of opportunistic microorganisms of 5 genera (pseudomonas, proteus, staphylococcus, klebsiella, escherichia) and the microbial status was carried out. a total of 854 patients from 10 profile departments of a surgical hospital were examined. population analysis and statistical methods of processing the results of the examination of 353 practically healthy subjects and 268 blood and plasma donors permit ...200212524998
contamination levels of in-use disinfectants in a teaching hospital in lagos, nigeria.in-use testing of the disinfectants; hibitane (5% w/v chlorhexidinegluconate), hibiscrub (4% w/v chlorhexidinegluconate), savlon (3% w/v chlorhexidine/cetrimide), hydrogen peroxide (6% w/v hydrogen peroxide with stabilizer) and a common household bleach jik (3.5% w/v sodium hypochlorite), was carried out over a two-month period at a university teaching hospital in nigeria. contamination levels were high with 82 (63.1%) of the 130 in-use disinfectants contaminated. however, a few of the stock sol ...200212518903
studies of certain factors affecting the microenvironment and microflora of the external ear of the dog in health and disease.a total of 187 dogs, 110 with clinical signs of otitis externa (oe), and 77 without history or clinical signs of oe, were examined microenvironment and microbiological analysis of their ear exudates made. the aural temperature and humidity of 160 dogs were measured. there were no significant difference between healthy dogs and oe dogs. german shepherd showed relatively lower temperature (p<0.01) and higher humidity (p<0.01). the mean log(10) number of microbial organisms of ears of oe dogs (4.16 ...200212520110
antimicrobial activity of feiii, cuii, agi, znii and hgii complexes of 2-(2-hydroxy-5-bromo/nitro-phenyl)-1h- and 2-(2-hydroxyphenyl)-5-methyl/chloro/nitro-1h-benzimidazoles.antimicrobial activity of 2-(2-hydroxyphenyl)-5-r5-1h-benzimidazoles, 2-(2-hydroxy-5-r5'-phenyl)-1h-benzimidazoles and their feiii, cuii, agi, znii and hgii nitrate complexes was tested toward staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella typhi, shigella flexneri, and proteus mirabilis. antifungal activity was tested against candida albicans. benzimidazole benzene ring substituents increase the antimicrobial activit ...200212503391
[pathogenicity islands in opportunistic enterobacteria].the presence of fragments of genomes hlya, hlyb, papah, papc, sfag, sfaa and kps mt, associated with the pathogenicity islands of escherichia coli, in clinical strains of other genera of the family enterobacteriaceae, has been experimentally evaluated with the use of pcr. the presence of dna fragments specific to the known genes of the pathogenicity clusters of e. coli in representatives of the genera citrobacter, enterobacter, hafnia, klebsiella, morganella, proteus, providencia, serratia and y ...200212506618
[dynamics of antibiotic resistance of purulent septic pathogens at an emergency facility].the predominant pathogens at the emergency medicine hospital were analysed and its resistance to antibiotics was investigated in dynamics. the susceptibility of the pathogens was analysed by the method of dynamic equalibration. statistically significant reduction of resistant bacteria isolation was demonstrated for 2 species of 7--that is s. aureus and e. faecalis regarding 4 antibiotics. the ratio of staphylococci resistant to cefazolin, ceftazidime, doxycyclin reduced, the ratio of enterococci ...200212515091
on the involvement of the water-polaron mechanism in energy trapping by reaction centers of purple bacteria.a locus for binding a mobile water molecule was searched for in the immediate vicinity of the special pair in the reaction center. using the proteus pc-program (a part of the grasp package) atomic structures of the reaction centers were analyzed in purple bacteria rhodopseudomonas viridis and rhodobacter sphaeroides. in both structures the loci for binding mobile water molecules were found at the distance of about 4.5 a from the middle of the special pair in the reaction center. the reorientatio ...200212495417
[amyloidosis associated with chronic granulomatous disease in a patient with a renal transplant and recurrent urinary tract infections].chronic granulomatous disease is a group of syndromes which share a defect in a component of the phagocyte nadph-oxidase complex. without this enzyme activity, phagocytic cells cannot produce superoxide, peroxide, and other potent microbicidal radicals, and are less able to kill ingested pathogens. the clinical picture is characterised by recurrent life-threatening bacterial and fungal infections and abnormal tissue granuloma formation. on the other hand, amyloidosis is a systemic disease with r ...200212497752
polysaccharide part of proteus mirabilis lipopolysaccharide may be responsible for the stimulation of platelet adhesion to collagen.lipopolysaccharide (lps, endotoxin) is a well-known causative agent of septic shock and disseminated intravascular coagulation. the action of lps is related to the activation of many types of cells, including blood platelets. we examined the effects of different lpss from proteus mirabilis (smooth form lps s1959 and rough forms lpss r110 and r45) and fragments of lps structure (lipid a and polysaccharide part) on platelet adhesion to collagen in the presence or absence of the phoinositide 3-kina ...200212487790
antimicrobial activity of psidium guajava l.psidium guajava aqueous bark and methanolic extracts were found to possess anti-bacterial activity.200212490238
necrotizing gangrene of the genitalia and perineum.necrotizing gangrene of the genitalia and perineum is a fulminant, life-threatening condition. this infection is usually polymicrobial and may be idiopathic or secondary to local trauma or surgery. histologically, it is characterized by obliterative endarteritis and thrombosis of the subcutaneous vessels, fascial necrosis, and leukocytic infiltration. mortality rates of 25-75% have been reported. most cases of necrotizing gangrene begin insidiously, with scrotal discomfort and malaise. later, er ...200212492967
urinary tract infection trends in latin american hospitals: report from the sentry antimicrobial surveillance program (1997-2000).urinary tract infections (uti) are one of the most common infectious diseases diagnosed in outpatients as well as in hospitalized patients. the objective of this study was to report the frequency of occurrence and antimicrobial susceptibility of uropathogens collected in latin america between 1997 to 2000 through the sentry antimicrobial surveillance program. antimicrobial susceptibility testing was performed and results interpreted using reference broth microdilution methods. in the 4 year peri ...200212493177
structure of the o-specific polysaccharide of proteus vulgaris o15 containing a novel regioisomer of n-acetylmuramic acid, 2-acetamido-4-o-[(r)-1-carboxyethyl]-2-deoxy-d-glucose.an acidic o-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of proteus vulgaris o15 and studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, roesy, and h-detected 1h,(13)c hmqc experiments. the polysaccharide was found to contain an ether of glcnac with lactic acid, and the following structure of the repeating unit was established:-->3)-alpha-d-glcpnac4(r-lac)6ac-(1-->2)-beta-d-glcpa-(1-->3)-alpha-l-6d ...200212493231
in vitro and in vivo pharmacodynamic properties of the fluoroquinolone ibafloxacin.the pharmacodynamic properties of a new veterinary fluoroquinolone antimicrobial agent, ibafloxacin, were evaluated. minimal inhibitory concentrations (mic), time-kill kinetics, postantibiotic effect (pae) and postantibiotic subminimal inhibitory concentration effects (pa-sme) were determined against pathogenic canine gram-negative and gram-positive bacterial isolates from dermal, respiratory and urinary tract infections. the synergistic interactions between ibafloxacin and its main metabolite, ...200212485346
tryptophan indole-lyase from proteus vulgaris: kinetic and spectral properties.an efficient method for purification of recombinant tryptophanase from proteus vulgaris was developed. catalytic properties of the enzyme in reactions with l-tryptophan and some other substrates as well as competitive inhibition by various amino acids in the reaction with s-o-nitrophenyl-l-cysteine were studied. absorption and circular dichroism spectra of holotryptophanase and its complexes with characteristic inhibitors modeling the structure of the principal reaction intermediates were examin ...200212460117
first occurrence of transferable extended-spectrum beta-lactamase hydrolyzing cefoperazone in multiresistant nosocomial strains of klebsiella pneumoniae from two hospitals in czech and slovak republics.the occurrence of positive synergy between antibiotic discs of amoxicillin/clavulanate and cefoperazone was registered in two klebsiella pneumoniae strains, isolated from hospitals in czech and slovak republic, indicating the presence of genes coding for an extended-spectrum beta-lactamase active also against cefoperazone, a broad-spectrum cephalosporin. sulbactam inhibited the hydrolysis of cefoperazone by cell-free lysates of these strains which substantiates its use in combination with cefope ...200212462425
effect of arginine-vasopressin on amoeba proteus: specific features of signal transmission. 200212469403
germline mutation of the tumour suppressor pten in proteus syndrome. 200212471211
epidemiology of bloodstream infection in nursing home residents: evaluation in a large cohort from multiple homes.this study sought to reevaluate the epidemiology of bloodstream infection in nursing home residents. the records of 166 nursing home residents admitted to an urban, public, university-affiliated hospital with 169 episodes of bloodstream infection between january 1997 and april 2000 were retrospectively reviewed. the most common organisms isolated were escherichia coli (27% of isolates), staphylococcus aureus (18%; 29% were methicillin-resistant strains), and proteus mirabilis (13%). there was mi ...200212471567
[comparison of fixation dio-sensitest and disk diffusion techniques for the antibiotic sensitivity of some enteric bacteria]. 200212476776
susceptibility of antibiotic-resistant gram-negative bacteria to biocides: a perspective from the study of catheter biofilms.bacteria resistant to both the agents deployed to prevent infections and those used to treat infections would be formidable nosocomial pathogens. the aim of this paper is to review the evidence that gram-negative bacteria resistant to antibiotics and biocides have emerged and been responsible for catheter-associated urinary tract infection. a study of patients undergoing intermittent bladder catheterization revealed that the frequent application of the antiseptic chlorhexidine to the perineal sk ...200212481841
in vitro activity of gemifloxacin against recent clinical isolates of bacteria in korea.gemifloxacin is an enhanced-affinity fluoroquinolone with broad-spectrum antibacterial activity. in korea, resistant bacteria are relatively more prevalent than in other industrialized countries. in this study, we studied the in vitro activities of gemifloxacin, gatifloxacin, moxifloxacin, levofloxacin, ciprofloxacin, and other commonly used antimicrobial agents against 1,689 bacterial strains isolated at four korean university hospitals during 1999-2000. minimum inhibitory concentrations (mics) ...200212482994
[use of mpo chromogenic culture medium for routine processing of urine cultures].the chromogenic culture medium, mpo, was compared to culture on cled (cystein, lactose, electrolyte-deficient) agar for the detection, enumeration and identification of urinary tract pathogens.200212372235
venous sinus thrombosis after proteus vulgaris meningitis and concomitant clostridium abscess formation.a 19-y-old woman presented with proteus vulgaris meningitis as a complication of chronic otitis media. despite treatment with ceftazidime and amikacin no clinical improvement was observed. cranial mri revealed right-sided mastoiditis/otitis media and venous sinus thrombosis. after mastoidectomy, repeat cranial mri demonstrated abscess formation in the venous sinuses. the abscess was drained. clostridium spp. was isolated from the abscess culture.200212374367
outcome of percutaneous nephrostomy for the management of pyonephrosis.the aim of this study was to evaluate the efficacy of percutaneous nephrostomy (pcn) drainage for the interim management of pyonephrosis.200212376218
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2000). i. susceptibility distribution].the bacterial strains isolated from patients diagnosed as having urinary tract infections (utis) in 10 institutions in japan were supplied between the period of august 2000 and july 2001. then, the susceptibilities of them to many kinds of antimicrobial agents were investigated. the number of them were 511 strains. the breakdown of these strains was gram-positive bacteria as 29.0% and gram-negative bacteria as 71.0%. susceptibilities of these bacteria to antimicrobial agents were as follows; van ...200212378869
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2000). ii. background of patients].five-hundred eighty eight bacterial strains isolated from 435 patients diagnosed as having urinary tract infections (utis) in 10 institutions in japan were supplied between august 2000 and july 2001. then, the clinical background of patients were investigated such as sex, age, and type of infections, infections and kind of bacteria, frequency of bacteria isolation by age and infections, bacteria and infections by timing of antibiotics administration, and bacteria and infections by surgical proce ...200212378870
[in vitro and in vivo antibacterial activities of pazufloxacin mesilate, a new injectable quinolone].we investigated the in vitro and in vivo antibacterial activities of pazufloxacin mesilate (pzfx mesilate), a new injectable quinolone, and obtained the following results. 1) the mic50 and mic90 values of pzfx against clinically isolated gram-positive and -negative bacteria, ranged from 0.0125 to 12.5 micrograms/ml and 0.025 to 100 micrograms/ml, respectively. pzfx showed broad spectrum activity. the antibacterial activities of pzfx against quinolone-susceptible, methicillin-resistant staphyloco ...200212378871
infectious background of patients with a history of acute anterior uveitis.to study the infectious background of patients with a history of acute anterior uveitis (aau) and healthy control subjects.200212379526
identification of a chromosome-borne expanded-spectrum class a beta-lactamase from erwinia persicina.from whole-cell dna of an enterobacterial erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a beta-lactamase gene was cloned and expressed in escherichia coli. it encoded a clavulanic-acid-inhibited ambler class a beta-lactamase, erp-1, with a pi value of 8.1 and a relative molecular mass of ca. 28 kda. erp-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from citrobacter koseri ...200212384342
is there a need for postoperative surveillance after day case groin surgery in children?there is increasing awareness of the value of day case paediatric surgery in fulfilling the needs of the community. is this cost effective, are there hidden complications and is there a need for routine follow-up? seven hundred and thirty-one children attending the red cross war memorial children's hospital day surgery unit over a 32-month period, and undergoing 912 procedures confined to the inguinoscrotal region, were enrolled in the study. surgical procedures performed included inguinal herni ...200212387217
comparison of three chromogenic agar plates for isolation and identification of urinary tract pathogens.to comparatively assess the performance of three chromogenic agar plates, cps id2, chromogenic uti, and usa, for the detection and enumeration of all urinary tract pathogens and the direct identification of escherichia coli, proteus mirabilis and enterococcus spp.200212390282
[psoas abscess. etiology and treatment].psoas abscess is in general a syndrome with low incidence. in the following, two cases are presented which describe the etiology, diagnosis, and treatment of primary and secondary retroperitoneal manifestations. the article mainly focuses on the different respective surgical procedures. because of its rareness, an overview of further cases discussed in the literature is provided.200212395166
characterisation of local isolates of enterobacteriaceae from turkey.20 local isolates of enterics belonging to the genera salmonella, enterobacter, proteus, citrobacter from human, chicken and/or egg were characterised for their antibiotic resistance patterns, plasmid profiles, phage types, outer membrane proteins, and lipopolysaccharide patterns. relatedness of these characteristics for epidemiological analysis was assessed. 18 (90%) strains were resistant to at least one antibiotic and those (multi-drug resistant ones) resisting to two or more antibiotics cons ...200212398294
infestation of fish-culturing communities with fish-borne bacteria: the ghanaian case.twenty different bacterial species were isolated from fishermen and members of communities associated with seven fish ponds with different fertilizer treatments, and an open system. the number and types of the bacterial species were different for the different ponds. generally, neither the geographical location of the ponds nor the type of fertilizer used could be related to the bacterial flora. escherichia coli was the predominant species in all the communities, while escherichia coli, klebsiel ...200212400540
serum levels of prophylactic cefazolin during cardiopulmonary bypass surgery.controversy exists regarding the appropriate prophylactic dose of cefazolin for coronary artery bypass grafting (cabg) surgery requiring cardiopulmonary bypass (cpb) because the effect of cpb on serum drug levels is poorly understood. current standards of prophylaxis are based primarily on empiric studies. few studies have attempted to quantify serum cefazolin levels in either cardiac or noncardiac surgeries. this study was conducted to measure and assess the adequacy of the intraoperative serum ...200212400766
[triclosan, a topical dermatologic agent. in vitro- and in vivo studies on the effectiveness of a new preparation in the new german formulary].triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) is an antiseptic suitable for formulation as a w/o emulsion. the objective of the present study was to explore its potential utility in atopic dermatitis and prophylactic skin care following leg eczema and leg ulcer treatment.200212402134
antimicrobial activity of dequalinium chloride against leading germs of vaginal infections.dequalinium chloride (cas 522-51-0) and povidone iodine (cas 25655-41-8) are known as antiseptic agents and used in the local treatment of vaginal infections. clotrimazole (cas 23593-75-1) is an anti-fungal drug and applied primarily in the therapy of vulvo-vaginal candidiasis and to a lesser extent in bacterial vaginosis and trichomoniasis. however, antimicrobial activities of those three agents in comparison to each other have not been reported so far. to address this issue the antimicrobial a ...200212404886
anti-salmonella activity of alkyl gallates.a series of alkyl gallates (3,4,5-trihydroxybenzoates) was synthesized and tested for their antibacterial activity against salmonella choleraesuis. nonyl (c(9)) and octyl (c(8)) gallates were noted to be the most effective against this food-borne bacterium, each with a minimum bactericidal concentration (mbc) of 12.5 microg/ml, followed by decyl (c(10)) gallate, with a mbc of 25 microg/ml. dodecyl (c(12)) gallate exhibited activity against s. choleraesuis, with a mbc of 50 microg/ml. propyl (c(3 ...200212405763
characterization of cmy-type beta-lactamases in clinical strains of proteus mirabilis and klebsiella pneumoniae isolated in four hospitals in the paris area.we isolated five clinical strains (three proteus mirabilis and two klebsiella pneumoniae) with beta-lactam resistance phenotypes consistent with production of an ampc-type beta-lactamase. the predicted amino acid sequences of the enzymes were typical of class c beta-lactamases. the enzymes were identified as cmy-2, cmy-4 and a new cmy-variant beta-lactamase, cmy-12. the ampc beta-lactamases from the two k. pneumoniae isolates were found to be encoded on self-transferable plasmids. the genes enco ...200212407124
modification of dienes mutual inhibition test for epidemiological characterization of pseudomonas aeruginosa isolates.pseudomonas aeruginosa is an important cause of community-associated and nosocomial infections related to exposure to aqueous environments. such infections often occur in the setting of a common-source outbreak, in which case epidemiological characterization of isolates may be necessary. in this preliminary study, a modification of the dienes mutual inhibition test, ordinarily used to assess the relatedness of swarming proteus mirabilis strains, was used to study 15 p. aeruginosa isolates, with ...200212409411
[fibrin-collagen substance as a vehicle for local application of antibacterial agents].inhibitory activity of fibrin-collagen substance (fcs) impregnated by various antibacterial agents against various microorganisms was investigated. it was demonstrated that antibacterials bind well with fcs, providing its sufficient antibacterial activity. determination of growth-inhibition zones showed that antibacterial agents diffuse into medium well and demonstrated the same activity as discs with antibiotics. for clinical practice it is recommended to use for impregnation the following drug ...200212422648
antimicrobial properties and phytochemical constituents of the leaves of african mistletoe (tapinanthus dodoneifolius (dc) danser) (loranthaceae): an ethnomedicinal plant of hausaland, northern nigeria.african mistletoe (tapinanthus dodoneifolius (dc) danser) called 'kauchi' in hausa is a hemi-plant parasite used ethnomedicinally by the hausa and the fulani tribes of northern nigeria as a remedy for several human and animal ailments that include stomach ache, diarrhoea, dysentery, wound and cancer. screening of the plant, obtained from 14 different hosts, revealed a wide spectrum of antimicrobial activities against certain multiple drug resistant bacterial and fungal isolates of farm animals. ...200212426091
indigoferabietone, a novel abietane diterpenoid from indigofera longeracemosa with potential antituberculous and antibacterial activity.from the stems of indigofera longeracemosa, a novel abietane diterpenoid, indigoferabietone was isolated. the structure was established by spectral techniques. the inhibitory activity of indigoferabietone (1) tested against mycobacterium tuberculosis and candida albicans, and the antibacterial activity tested against staphylococcus aureus, proteus vulgaris, and escherichia coli were found to be significant.200212426956
the inner-core lipopolysaccharide biosynthetic waae gene: function and genetic distribution among some enterobacteriaceae.to determine the function of the waae gene in the biosynthesis of the inner-core lps of klebsiella pneumoniae, a waae non-polar mutant has been constructed. data obtained from the comparative chemical analysis of lps samples obtained from the wild-type, the mutant strain and the complemented mutant demonstrated that the waae gene is involved in substitution of alpha-l-glycero-d-manno-heptopyranose i (l,d-heppi) at the o-4 position by a beta-d-glucopyranose (beta-d-glcp) residue. in addition, dna ...200212427940
effect of resveratrol, a natural polyphenolic compound, on platelet activation induced by endotoxin or thrombin.resveratrol (3, 4', 5-trihydroxystilbene), a natural polyphenol, is found in some plants that are used in human nutrition. grapes are a major source for resveratrol, and a significant amount can also be found in red wine. several experimental studies have demonstrated biological properties of resveratrol, especially its anti-inflammatory, antioxidant, anti-platelet and antitumor effects. in the present study, we investigated the first step of platelet activation-platelet adhesion stimulated by l ...200212431480
lower genital tract infections in diabetic women.the influence of glucose metabolism is seen in many infectious diseases, making diabetic patients more vulnerable to sepsis and other serious sequelae of bacterial invasion. vaginal candidiasis is a common problem if the glycemia is poorly controlled. the level of glucose concentration in the blood after ingestion of sugar seems to explain an increased likelihood of recurrent infection. specific immune aberrations, such as an elevated t-helper 2 response and a blunted t-helper 1 response, leadin ...200212433331
distribution of extended-spectrum beta-lactamases in clinical isolates of enterobacteriaceae in vietnam.among 730 escherichia coli, 438 klebsiella pneumoniae, and 141 proteus mirabilis isolates obtained between september 2000 and september 2001 in seven hospitals in ho chi minh city, vietnam, 26.6% were resistant to ceftazidime, 30% were resistant to cefotaxime, 31.5% were resistant to ceftriaxone, 15.9% were resistant to cefoperazone, and 6% were resistant to cefepime. resistance to imipenem was found in 5.6% of the isolates. in 55 strains producing extended-spectrum beta-lactamases (32 e. coli i ...200212435670
biochemical characterization of tem-92 extended-spectrum beta-lactamase, a protein differing from tem-52 in the signal peptide.a bla(tem-92) gene was cloned from a proteus mirabilis isolate and expressed in escherichia coli. production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. clavulanate and penicillanic acid sulfone ...200212435705
pathological and therapeutic significance of cellular invasion by proteus mirabilis in an enterocystoplasty infection stone model.proteus mirabilis infection often leads to stone formation. we evaluated how bacterium-mucin adhesion, invasion, and intracellular crystal formation are related to antibiotic sensitivity and may cause frequent stone formation in enterocystoplasties. five intestinal (caco-2, ht29, ht29-18n2, ht29-fu, and ht29-mtx) and one ureter cell line (sv-huc-1) were incubated in artificial urine with five proteus mirabilis strains. fluorescence-activated cell sorting (facs), laser scanning microscopy, and el ...200212438382
a muscular lesion suggestive of focal myositis in a child with proteus syndrome.proteus syndrome is a polymorphic hamartomatous disorder associated with a broad spectrum of rare congenital malformations. many neoplasms have been linked to this condition, including lipomas, lymphangiomas, and hemangiomas. the authors describe a case of a congenital muscular lesion in a child with proteus syndrome. this mass was painless but was growing out of proportion to her leg musculature. a magnetic resonance imaging scan and incisional biopsy showed findings consistent with focal myosi ...200212439276
isolation of lacz fusions to proteus mirabilis genes regulated by intercellular signaling: potential role for the sugar phosphotransferase (pts) system in regulation.using a mini-tn5lacz1 reporter transposon, lacz fusions have been identified in proteus mirabilis that are activated by the accumulation of self-produced extracellular signals. genes identified by this approach include putative homologs of pgm, nlpa and two genes of unknown function. the extracellular signal(s) involved in activation were resistant to the effects of acid and alkali. the signal required for activation of (nlpa) cma482::lacz was sensitive to protease, suggesting the signal is a pe ...200212445644
brain abscess caused by aerobic gram-negative bacilli: clinical features and therapeutic outcomes.thirty-three patients (24 males and nine females) with brain abscesses resulting from infection by aerobic gram-negative bacilli were identified at kaohsiung chang gung memorial hospital over a period of 14 years. of these, 23 cases developed spontaneously, with the remaining ten postneurosurgery. the organisms most frequently involved were klebsiella (k.) pneumoniae, pseudomonas aeruginosa, escherichia coli and proteus species and included some rare pathogens, such as salmonella and enterobacte ...200212445926
brain abscess complicating cerebral infarct.we report a case of a 68-year-old man who suffered ischemic strokes in the left middle cerebral artery territory and three months later, following urosepsis, developed a cerebral abscess in the infarcted area.200212446296
the helicobacter pylori flba flagellar biosynthesis and regulatory gene is required for motility and virulence and modulates urease of h. pylori and proteus mirabilis.helicobacter pylori and proteus mirabilis ureases are nickel-requiring metallo-enzymes that hydrolyse urea to nh3 and co2. in both h. pylori and in an escherichia coli model of h. pylori urease activity, a high affinity nickel transporter, nixa, is required for optimal urease activity, whereas the urea-dependent urer positive transcriptional activator governs optimal urease expression in p. mirabilis. the h. pylori flba gene is a flagellar biosynthesis and regulatory gene that modulates urease a ...200212448680
structural and serological characterization of the lipopolysaccharide from proteus penneri 20 and classification of the cross-reacting proteus penneri strains 10, 16, 18, 20, 32 and 45 in proteus serogroup o17.o-specific polysaccharide (o-antigen) of the lipopolysaccharide of proteus penneri 20 was studied using sugar analysis along with various one- and two-dimensional nmr spectroscopy techniques. the following structure of the polysaccharide was established: [formula: see text] it has the same carbohydrate backbone structure as that described earlier for p. penneri 16, in which the positions of the o-acetyl groups have not been determined. p. penneri 20 o-antiserum showed a strong cross-reactivity w ...200212455869
polymerase chain reaction for the rapid diagnosis of tuberculous meningitis.(i)200214510337
antibiotic sensitivity pattern of the bacteria isolated from nosocomial infections in icu.the incidence of nosocomial infections in icu is 4-5 times greater than in general ward. critically ill patients are always at higher risk of developing nosocomial infections with resistant strains. this study is an attempt to know the antibiotic sensitivity pattern of the common isolates in icu. samples comprising urine, blood, endotracheal secretions and throat swabs were collected from 102 icu patients of them, 56 patients showed evidence of nosocomial infection (54.9%), from whom 120 differe ...200214710856
[ study of exo-enzymatic profile and cytotoxic effect of bacterial culture supernatants in different growth phase].bacterial quorum-sensing represents an ubiquitary regulating system in which the pheromones (small molecules with different chemical structures, i.e. homoserin-lactones, octapeptides, aminoacids) act as extracellular mediators of signaling and intercellular communication. this chemical system is implicated in the regulation of different physiological processes dependent on the cell density (i.e. biolumniscence, virulence factors expression, sporulation, conjugation, antibiotic secretion etc). it ...200215085600
asymptomatic bacteriuria among outpatients with diabetes mellitus in an urban black population.to determine the prevalence of asymptomatic bacteriuria (asb) in individuals afflicted by diabetes mellitus; the antibiotic susceptibility of the microbial isolates and the association of host factors with asb.200214562524
antibacterial studies of cefixime copper, zinc and cadmium complexes.antibacterial studies of metal complexes of cefixime, with copper zinc and cadmium have been carried out by observing the minimum inhibitory concentration (mic) and by measuring the zone of inhibition of the complexes and compared with the parent cephalosporin against both gram negative and gram positive microorganisms. various microorganisms used were staphylococcus aureus, e. coli, klebsiella and proteus species. for mic observation, serial dilution method was employed and zone sizes were dete ...200216414863
clarithromycin synergism with essential and trace elements.in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various macrolide antibiotics, the synergistic or antagonistic behavior of clarithromycin metal complexes has been studied and compared with the parent drug. metal complexes of clarithromycin with magnesium, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc and cadmium has been investigated for their antibacterial activity and compared with clarithromycin by observin ...200216414867
microbial, chemical and physical aspects of citrus waste composting.citrus waste supplemented with calcium hydroxide and with a c/n ratio of 24:1, ph of 6.3 and moisture content of 60% was composted by piling under shelter. with regular turning over of the pile and replenishment of moisture, the thermic phase lasted for 65-70 days and composting was completed after 3 months. compost thus prepared had an air-filled porosity of 14%, water-holding capacity of 590 ml l(-1), bulk density of 1.05 g cm(-3) and conductivity of 480 ms m(-1). phosphorus content (in mg l(- ...200211708757
visualization of proteus mirabilis within the matrix of urease-induced bladder stones during experimental urinary tract infection.the virulence of a urease-negative mutant of uropathogenic proteus mirabilis and its wild-type parent strain was assessed by using a cba mouse model of catheterized urinary tract infection. overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type p. mirabilis. at a high inoculum, the urease-negative mutant successfully colonized bladders of catheterized mice but did not cause urolithiasis and was still severely attenuated in its ability to ...200211748205
occurrence of extended-spectrum beta-lactamases in members of the family enterobacteriaceae in italy: implications for resistance to beta-lactams and other antimicrobial drugs.an italian nationwide survey was carried out to assess the prevalences and the antimicrobial susceptibilities of members of the family enterobacteriaceae producing extended-spectrum beta-lactamases (esbls). over a 6-month period, 8,015 isolates were obtained from hospitalized patients and screened for resistance to extended-spectrum cephalosporins and monobactams. on the basis of a synergistic effect between clavulanate and selected beta-lactams (ceftazidime, aztreonam, cefotaxime, cefepime, and ...200211751134
postneurosurgical meningitis due to proteus penneri with selection of a ceftriaxone-resistant isolate: analysis of chromosomal class a beta-lactamase huga and its lysr-type regulatory protein hugr.we report on a case of a postneurosurgical meningitis due to ceftriaxone-susceptible proteus penneri, with selection of a ceftriaxone-resistant isolate following treatment with ceftriaxone. the isolates presented identical patterns by pulsed-field gel electrophoresis and produced a single beta-lactamase named huga with an isoelectric point of 6.7. the ceftriaxone-resistant isolate hyperproduced the beta-lactamase (increase in the level of production, about 90-fold). the sequences of the huga bet ...200211751137
solid-phase synthesis of new saphenamycin analogues with antimicrobial activity.an array of 12 new saphenamycin analogues modified at the benzoate moiety was synthesized on solid support. synthesis commenced with a chemoselective anchoring of saphenic acid through the carboxyl group to a 2-chlorotrityl functionalized polystyrene resin. the secondary alcohol was acylated in parallel with a series of differently substituted benzoic acid derivatives. treatment with tfa-ch(2)cl(2) (5:995) released the expected saphenamycin analogues into solution. these new analogues were purif ...200211755347
structural and serological relatedness of the o-antigens of proteus penneri 1 and 4 from a novel proteus serogroup o72.o-specific polysaccharides (o-antigens) of the lipopolysaccharides (lps) of proteus penneri strains 1 and 4 were studied using sugar analysis, (1)h and (13)c nmr spectroscopy, including 2d cosy, h-detected (1)h,(13)c hmqc, and rotating-frame noe spectroscopy (roesy). the following structures of the tetrasaccharide (strain 1) and pentasaccharide (strain 4) repeating units of the polysaccharides were established: [reaction: see text]. in the polysaccharide of p. penneri strain 4, glycosylation wit ...200211784330
retrospective study of children with acute pyelonephritis. evaluation of bacterial etiology, antimicrobial susceptibility, drug management and imaging studies.the purpose of the study was to investigate the management of pyelonephritis in a large italian pediatric population. a total of 1,333 patients (36% male) were considered. escherichia coli was the most frequently isolated agent (89.9%), followed by proteus mirabilis (3.6%) and klebsiella oxytoca (2.1%). 27% of microorganisms were resistant to amoxicillin, 4% to amoxicillin/clavulanic acid, 11% to trimethoprim-sulfamethoxazole, 2.4% to gentamicin and less than 2% to ceftazidime. despite this resi ...200211744799
susceptibility to fluoroquinolones among commonly isolated gram-negative bacilli in 2000: trust and tsn data for the united states. tracking resistance in the united states today. the surveillance network.from january to may 2000, as part of the tracking resistance in the united states today (trust) surveillance initiative, clinical isolates of enterobacteriaceae (n=2519) and non-fermentative gram-negatives (n=580) were prospectively collected from 26 hospital laboratories across the united states. isolates were tested for susceptibility to three fluoroquinolones (ciprofloxacin, levofloxacin, gatifloxacin) and seven other agents. in addition, data for the same period were collected from the surve ...200211814764
novel bacterial membrane surface display system using cell wall-less l-forms of proteus mirabilis and escherichia coli.we describe a novel membrane surface display system that allows the anchoring of foreign proteins in the cytoplasmic membrane (cm) of stable, cell wall-less l-form cells of escherichia coli and proteus mirabilis. the reporter protein, staphylokinase (sak), was fused to transmembrane domains of integral membrane proteins from e. coli (lactose permease lacy, preprotein translocase secy) and p. mirabilis (curved cell morphology protein ccma). both l-form strains overexpressed fusion proteins in amo ...200211823186
molecular characterization of extended-spectrum beta-lactamases produced by nosocomial isolates of enterobacteriaceae from an italian nationwide survey.extended-spectrum beta-lactamases (esbls) are widespread in hospital settings worldwide. the present investigation was undertaken to assess the distribution and prevalence of esbls belonging to the tem and shv families in 448 esbl-producing clinical isolates of enterobacteriaceae collected from 10 different italian hospitals. the natures of tem and shv determinants were identified by direct sequencing of pcr-amplified genes. tem-52 and shv-12 were the most common variants, and they were found in ...200211825979
[in vitro antimicrobial activity of glutaraldehyde plus o-phenylphenol association (ratio 2:1)].the correct use of antiseptics, disinfectants and sterilization processes to inactivate or remove micro-organisms is an essential component of an effective infection control program. to reach this result the use of phenolic detergent-disinfectants which work well as a presoak has always been suggested in lieu of sterilization involving submerging instruments in a properly prepared glutaraldehyde solution for about 6 to 10 hrs. this study investigated the in vitro effectiveness of disinfectant so ...200211845118
current presentation and optimal surgical management of sternoclavicular joint infections.infection of the stemoclavicular joint is unusual, and treatment of this entity has not been standardized. we sought to characterize the current presentation and optimal management of this disease.200211845854
the migration of proteus mirabilis and other urinary tract pathogens over foley catheters.to examine the ability of organisms that infect the catheterized urinary tract to migrate over the surfaces of foley catheters.200211849161
characterization of a new extended-spectrum beta-lactamase (tem-87) isolated in proteus mirabilis during an italian survey.a new natural tem derivative, named tem-87, was identified in a proteus mirabilis isolate from an italian hospital. compared to tem-1, tem-87 contains the following mutations: e104k, r164c, and m182t. kinetic analysis of tem-87 revealed extended-spectrum activity against oxyimino cephalosporins (preferentially ceftazidime) and aztreonam. expression of blatem-87 in escherichia coli decreased the host susceptibility to these drugs.200211850290
genital tract tumors in proteus syndrome: report of a case of bilateral paraovarian endometrioid cystic tumors of borderline malignancy and review of the literature.proteus syndrome is a rare, sporadic disorder that causes postnatal overgrowth of multiple tissues in a mosaic pattern. characteristic manifestations include: overgrowth and hypertrophy of limbs and digits, connective tissue nevus, epidermal nevus and hyperostoses. various benign and malignant tumors and hamartomas may complicate the clinical course of patients with the syndrome. commonly encountered tumors include hemangiomas, lymphangiomas and lipomas. tumors of the genital tract occur less of ...200211850547
[identification of plasmid-encoded cephalosporinase acc-1 among various enterobacteria (klebsiella pneumoniae, proteus mirabilis, salmonella) isolated from a tunisian hospital (sfax 997-2000)].because a multiresistant k. pneumoniae outbreak detected in an intensive care unit of a parisian hospital, combined to the production of the plasmid-encoded cephalosporinase acc-1, a probable importation via a patient was suggested from another country (tunisia). the investigation was conducted to examine 35 clinical strains of enterobacteria resistant to ceftazidime without synergy towards augmentin. other test of synergy with two inhibitors, brl 42715, ro 48-5545 was performed by diffusion met ...200211873633
structure of the o-polysaccharide and classification of proteus mirabilis strain g1 in proteus serogroup o3.the o-chain polysaccharide of the lipopolysaccharide (lps) of a previously nonclassified strain of proteus mirabilis termed g1 was studied by sugar analysis and 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, rotating-frame noe (roesy), h-detected 1h,13c hmqc, and heteronuclear multiple-bond correlation (hmbc) experiments. the following structure of the polysaccharide was established: [carbohydrate structure: see text] where d-gala6(l-lys) stands for n(alpha)-(d-galacturonoyl)-l-lysine. t ...200211874454
protean pten: form and function.germline mutations distributed across the pten tumor-suppressor gene have been found to result in a wide spectrum of phenotypic features. originally shown to be a major susceptibility gene for both cowden syndrome (cs), which is characterized by multiple hamartomas and an increased risk of breast, thyroid, and endometrial cancers, and bannayan-riley-ruvalcaba syndrome, which is characterized by lipomatosis, macrocephaly, and speckled penis, the pten hamartoma tumor syndrome spectrum has broadene ...200211875759
craniofacial and dental manifestations of proteus syndrome: a case report.the proteus syndrome is a rare congenital hamartomatous condition that is characterized by a wide range of malformations, sometimes involving the face. common manifestations include partial gigantism, congenital lipomas, and plantar hyperplasia. in this report we describe the craniofacial clinicopathological development in a girl with proteus syndrome from age 6 to 20 years. the patient had pronounced hemifacial hypertrophy, exostoses in the left parietal region, and enlargement of the inferior ...200211879083
treatment of chronic suppurative otitis media with ofloxacin in hydroxypropyl methylcellulose ear drops: a clinical/bacteriological study in a rural area of malawi.chronic suppurative otitis media in young children is a major problem in africa, with socio-economic consequences at a later age. common treatment regimens with antibiotics are expensive and often not practically feasible. therefore, a project was started to develop a low-cost and effective treatment in a rural area of malawi by studying the clinical efficacy of an inexpensive application regimen of ofloxacin (0.075%) in hydroxypropyl methylcellulose (1.5%) ear drops. in earlier studies with thi ...200211879929
single-dose fluoroquinolone therapy of acute uncomplicated urinary tract infection in women: results from a randomized, double-blind, multicenter trial comparing single-dose to 3-day fluoroquinolone regimens.to compare the efficacy and safety of single-dose and 3-day fluoroquinolone treatment of uncomplicated urinary tract infection (uti).200211880065
detection and mutation of a luxs-encoded autoinducer in proteus mirabilis.quorum sensing regulates the expression of virulence factors in a wide variety of pathogenic bacteria. this study has shown that proteus mirabilis harbours a homologue of luxs, a gene required for the synthesis of the quorum sensing autoinducer 2 (ai-2). ai-2 activity is expressed during and is correlated with the initiation of swarming migration on agar surfaces. the p. mirabilis luxs locus was cloned and a luxs(-) strain constructed by allelic-exchange mutagenesis. while lacking ai-2 activity, ...200211882712
proteus syndrome.proteus syndrome is a rarely described dysplasia syndrome of the group of congenital hamartomas that arises from mosaic mutation. an extraordinary case history including imaging studies will be reported. this 17-year-old girl suffered from cachexia, lifelong chronic obstipation, different dysplasias, and lipomatous tumor-like lesions. the following findings were marked: macrodactyly, nevi, hemihypertrophy, aggressive lipomatosis, hemangiomas of the spleen, and skull and cerebral malformations. a ...200211884784
the characterization of bacterial isolates from acute otitis media in ile-ife, southwestern nigeria.the incidence of acute otitis media (aom) in a comprehensive healthcare setting was investigated in ile-ife, nigeria. out of the 617 children examined, 53 (11.64 per cent) suffered from the condition based on the criteria used. staphylococci constituted the predominant organisms associated with the condition with staphylococcus aureus (25.0 per cent) being the most frequent single microbe recovered from the subjects. this was followed by proteus mirabilis (16.2 per cent), staphylococcus sp. (8.8 ...200211866331
rapid antimicrobial susceptibility testing of urinary tract isolates and samples by flow cytometry.a multiparametric flow cytometry antimicrobial susceptibility test was developed and its performance was evaluated on clinical urine isolates and samples in comparison with standard methods. alterations in cytoplasmic membrane integrity were monitored by propidium iodide, and the anionic probe bis-(1,3-dibutylbarbituric acid) trimethine oxonol (dibac4(3)) was used to measure changes in membrane potential. microbial size and cellular content were analysed by light scattering. twelve antibiotics w ...200211871613
combined coliform and anaerobic infection of the lacrimal sac.a case is reported of combined coliform and anaerobic bacterial infection of the lacrimal sac, a condition of which there is only one other published case report. in addition, a literature review is presented of the bacteriology of acute dacryocystitis as it applies to this case. recommendations for the microbiological investigation and management of acute dacryocystitis are made.200211885798
gstb1-1 from proteus mirabilis: a snapshot of an enzyme in the evolutionary pathway from a redox enzyme to a conjugating enzyme.the native form of the bacterial glutathione transferase b1-1 (ec ) is characterized by one glutathione (gsh) molecule covalently linked to cys-10. this peculiar disulfide, only found in the beta and omega class glutathione s-transferases (gsts) but absent in all other gsts, prompts questions about its role and how gsh can be activated and utilized in the reaction normally performed by gsts. stopped-flow and spectroscopic experiments suggest that, in the native enzyme (gstb1-1ox), a second gsh m ...200211889135
antimicrobial resistance in gram-negative isolates from european intensive care units: data from the meropenem yearly susceptibility test information collection (mystic) programme.susceptibility data were collected for 6243 gram-negative isolates from 29 european icus participating in the meropenem yearly susceptibility test information collection (mystic) programme (1997-2000). the most commonly isolated bacteria were pseudomonas aeruginosa (22.5%), escherichia coli (19.8%), klebsiella pneumoniae (10.4%), and enterobacter cloacae (7.7%). the incidence of extended-spectrum beta-lactamase-producers was higher in turkish, russian and italian icus (27.9-39.6%) than in other ...200211892895
histopathologic and bacteriologic evaluations of cellulitis detected in legs and caudal abdominal regions of turkeys.the objective of this study was to identify the causative agent of cellulitis in turkeys. eighteen flocks from nine producers were sampled at the local processing plant, and 37 birds with cellulitis on legs or caudal thoracic area were obtained. none of the 37 birds with cellulitis had lesions in other organs. on gross examination, lesions were categorized into two groups: cellulitis with unopened skin lesions (type a) and cellulitis with opened skin lesions (type b). histopathologically, cellul ...200211922333
emerging extended-spectrum beta-lactamases in proteus mirabilis.beta-lactamase production was detected in 147 (52%) of 282 consecutive nonduplicate proteus mirabilis isolates obtained over a 1-year period from the s. matteo hospital of pavia (northern italy). seventy isolates (48% of the beta-lactamase producers) were found to produce extended-spectrum beta-lactamases (esbls), identified as per-1 (first report in this species) and tem-52 in 52 and 18 isolates, respectively. analysis of clonal diversity of the esbl producers suggested different spreading patt ...200211923394
[use of the multi-component vp-4 vaccine to prevent acute respiratory diseases at preschool children's establishments].the multicomponent vp-4 vaccine from klebsiella pneumonia, staphyloccocus aureus, proteus vulgaris, escherichia coli antigens was used to prevent acute respiratory diseases (ard) at preschoolchildren's establishments. prevention was effected during an influenza epidemic with a drastic rise in the incidence of ard. the vp-4 use involved 3 nasal and 6-9 oral administrations of the drug at an interval of 3-4 days. the duration of the course was 26 +/- 4 days. the preventive action of vp-4 on the in ...200211924123
properties and utility of the peculiar mixed disulfide in the bacterial glutathione transferase b1-1.bacterial glutathione transferases appear to represent an evolutionary link between the thiol:disulfide oxidoreductase and glutathione transferase superfamilies. in particular, the observation of a mixed disulfide in the active site of proteus mirabilis glutathione transferase b1-1 is a feature that links the two families. this peculiar mixed disulfide between cys10 and one gsh molecule has been studied by means of esr spectroscopy, stopped-flow kinetic analysis, radiochemistry, and site-directe ...200211926831
staghorn calculus endotoxin expression in sepsis.staghorn calculi are infrequent and generally are infected stones. struvite or apatite calculi are embedded with gram-negative bacteria, which can produce endotoxin. sepsis syndrome may occur after surgical therapy or endoscopic manipulation of infected or staghorn calculi. sepsis, which can occur despite perioperative antibiotic use, may be due to bacteremia or endotoxemia. we present a child with an infected staghorn calculus who developed overwhelming sepsis and died after percutaneous stone ...200211927328
sensory, chemical and bacteriological changes in vacuum-packed pressurised squid mantle (todaropsis eblanae) stored at 4 degrees c.sensory, chemical and bacteriological changes were studied in vacuum-packed squid mantles (todaropsis eblanae) that were pressurised at 150, 200, 300 and 400 megapascal (mpa) for 15 min at ambient temperature and stored at 4 degrees c. sensory analysis showed that the higher the pressurisation the longer the shelf-life. thus, the lot pressurised at 400 mpa was rejected after 28 days' storage compared with 7 days for the untreated lot. the chemical results generally corresponded with the sensory ...200211929163
prevalence of extended spectrum beta-lactamase (esbl)-producing clinical isolates in the asia-pacific region and south africa: regional results from sentry antimicrobial surveillance program (1998-99).the frequency of occurrence of esbl-producing clinical strains varies widely in distinct geographic areas. the objective of this study was to describe the frequency of occurrence, the preferred substrate, and the co-resistance patterns of the esbl-producing isolates collected from the asia-pacific region and south africa through the sentry antimicrobial surveillance program between january 1998 and december 1999. a total of 1,377 escherichia coli, 678 klebsiella pneumoniae, and 138 proteus mirab ...200211929691
presence of rickettsia helvetica in granulomatous tissue from patients with sarcoidosis.in samples obtained during the autopsies of 2 patients with sarcoidosis, genetic material from rickettsia helvetica was detected by polymerase chain reaction, and histologic and immunohistochemical examination (using 3 different antibodies) of the polymerase chain reaction-positive tissues showed different degrees of granuloma formation and presence of rickettsia-like organisms predominantly located in the endothelium and macrophages. electron microscopic examination clearly identified and demon ...200211930323
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