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[total infarction of the penis caused by entrapment in a plastic bottle].penile strangulation injuries are rare emergency situations, but need a fast urological emergency management for decompression of the penis and for removing of the foreign bodies. strangulation objects can be metallic or non-metallic objects and are usually used for erotic or autoerotic purposes, for masturbation or increasing erection. we report a case of a 62-year old male with a total infected gangrene of the penis caused by a strangulation of a plastic bottle neck which continued 14 days. a ...200415205739
endophthalmitis after penetrating keratoplasty: microbiologic spectrum and susceptibility of isolates.to present the microbial spectrum and susceptibilities of isolates in endophthalmitis following penetrating keratoplasty.200414962427
histamine-related hygienic qualities and bacteria found in popular commercial scombroid fish fillets in taiwan.to determine the histamine-related hygienic qualities and bacteria of scombroid fish fillets sold in traditional retail markets, 61 samples were collected from northern and southern taiwan. it was found that the content of volatile base nitrogen in most samples was below 25 mg/100 g, which is the regulatory level in taiwan. the ratio of unacceptable samples/total samples for aerobic plate count and escherichia coli was 100% and 15% in northern samples and 100% and 20% in southern samples, respec ...200414968980
d- and l-aspartic acids: new non-sugar components of bacterial polysaccharides.for the first time in bacterial polysaccharides, residues of d- and l-aspartic acids were identified as n-acyl substituents of 4-amino-4,6-dideoxy-d-glucose in the o-antigens of enterobacteria of the genera providencia and proteus.200414972025
isolation and characterization of integron-containing bacteria without antibiotic selection.the emergence of antibiotic resistance among pathogenic and commensal bacteria has become a serious problem worldwide. the use and overuse of antibiotics in a number of settings are contributing to the development of antibiotic-resistant microorganisms. the class 1 and 2 integrase genes (inti1 and inti2, respectively) were identified in mixed bacterial cultures enriched from bovine feces by growth in buffered peptone water (bpw) followed by integrase-specific pcr. integrase-positive bacterial co ...200414982773
longitudinal trends in fluoroquinolone resistance among enterobacteriaceae isolates from inpatients and outpatients, 1989-2000: differences in the emergence and epidemiology of resistance across organisms.we conducted a 12-year study to identify and compare trends in annual prevalence of fluoroquinolone (fq) resistance among enterobacteriaceae isolates obtained from inpatients and outpatients in our health care system. a total of 46,070 clinical enterobacteriaceae isolates underwent susceptibility testing. although there were significant increases in inpatient fq resistance for all enterobacteriaceae, fq resistance trends differed significantly across enterobacteriaceae (p<.001). for isolates obt ...200414986249
different susceptibilities of staphylococcus and gram-negative rods to epigallocatechin gallate.we examined the antibacterial effects of epigallocatechin gallate (egcg, the main constituent of tea catechins) against various strains of staphylococcus and gram-negative rods. compared to the minimum inhibitory concentrations (mics) of egcg against s. aureus, s. epidermidis, s. hominis, and s. haemolyticus (50-100 micro g/ml), higher mics (>or=800 micro g/ml) were observed against gram-negative rods, including escherichia coli, klebsiella pneumoniae, salmonella typhi, proteus mirabilis, pseudo ...200414991521
four-year survey of urinary tract infections in calves in israel.the prevalence of urinary tract infections in calves aged seven days to three months in three dairy cattle herds ranged from 0.5 to 1.6 per cent, with an average of 1.1 per cent. the mortality rate reached 16.1 per cent. the morbidity rate of the female calves was 1.4 per cent and that of the male calves 0.8 per cent. the bacteria isolated from urine, and from vaginal and preputial swabs were escherichia coli (35 per cent), corynebacterium renale (14 per cent), plasma coagulase-negative staphylo ...200414994858
structure of the neutral o-polysaccharide and biological activities of the lipopolysaccharide of proteus mirabilis o20.mild acid degradation of the lipopolysaccharide (lps) of proteus mirabilis o20 resulted in depolymerisation of the o-polysaccharide to give a repeating-unit pentasaccharide. a polysaccharide was obtained by o-deacylation of the lps followed by nitrous acid deamination. the derived pentasaccharide and polysaccharide were studied by nmr spectroscopy, including 2d 1h,1h cosy, tocsy, roesy, 1h,13c hmqc and hmqc-toscy experiments, along with chemical methods, and the following structure of the repeat ...200415013399
composition and the in vitro antimicrobial activities of the essential oils of some thymus species.the genus thymus (lamiaceae) is represented by 38 species (64 taxa) in turkey, and 24 of which are endemic to turkey. aerial parts of thymus longicaulis subsp. chaubardii var. chaubardii, t. zygioides var. lycaonicus, t. longicaulis subsp. longicaulis var. subisophyllus and t. pulvinatus collected from three different localities in balikesir province were subjected to hydrodistillation to yield essential oils which were subsequently analysed by gc and gc/ms. the main constituents of the oils wer ...200415018057
current issues in the management of urinary tract infections: extended-release ciprofloxacin as a novel treatment option.symptomatic urinary tract infections (utis) are a major public health concern in the developed world, accounting for almost 8 million annual outpatient and emergency department visits in the us alone, while also representing one of the most common hospital-acquired infections. the vast majority of uncomplicated utis are caused by the gram-negative bacillus escherichia coli, with other pathogens including enterococci, staphylococcus saprophyticus, klebsiella spp. and proteus mirabilis. effective ...200415018591
the status of antimicrobial resistance in taiwan among gram-negative pathogens: the taiwan surveillance of antimicrobial resistance (tsar) program, 2000.in a nationwide surveillance of antimicrobial resistance (taiwan surveillance of antimicrobial resistance, tsar), isolates were collected from 21 medical centers and regional hospitals throughout taiwan over a three-month period in 2000 (tsar ii). this report summarizes susceptibility data of 7 common gram-negative bacilli (escherichia coli, klebsiella pneumoniae, serratia marcescens, proteus mirabilis, enterobacter cloacae, pseudomonas aeruginosa, and acinetobacter baumannii) in the tsar ii col ...200415023432
ascending to a uti vaccine? 200415040255
the possible influence of micro-organisms and putrefaction in the production of ghb in post-mortem biological fluid.in recent years, the post-mortem production of the drug of abuse gamma-hydroxybutyric acid (ghb) in biological fluids (e.g. blood and urine) has caused various interpretative problems for toxicologists. previously, other researchers have shown certain microbial species (pseudomonas spp. and clostridium aminobutyricum) possess the necessary enzymes to convert gaba to ghb. a preliminary investigation involving putrefied post-mortem blood indicated there was no observed relationship between "endoge ...200415040914
tyrosine phenol-lyase and tryptophan indole-lyase encapsulated in wet nanoporous silica gels: selective stabilization of tertiary conformations.the pyridoxal 5'-phosphate-dependent enzymes tyrosine phenol-lyase and tryptophan indole-lyase were encapsulated in wet nanoporous silica gels, a powerful method to selectively stabilize tertiary and quaternary protein conformations and to develop bioreactors and biosensors. a comparison of the enzyme reactivity in silica gels and in solution was carried out by determining equilibrium and kinetic parameters, exploiting the distinct spectral properties of catalytic intermediates and reaction prod ...200415044726
antibacterial and antipeptide antibodies in japanese and finnish patients with rheumatoid arthritis.it has been suggested that proteus infection may be involved in the pathogenesis of rheumatoid arthritis (ra). bacterial and peptide immune responses in patients with ra and other control subjects were investigated in two geographically different populations. serum samples from finnish patients with early ( n=72) and advanced ( n=27) ra and 30 finnish healthy controls, as well as from japanese ra patients from two different locations: tokyo ( n=30) and otsu ( n=30), 18 patients with systemic lup ...200415045628
assay method for antitumor l-methionine gamma-lyase: comprehensive kinetic analysis of the complex reaction with l-methionine.l-methionine gamma-lyase (ec 4.4.1.11) is a pyridoxal 5'-phosphate-dependent multifunctional enzyme. measuring the initial velocity of alpha-ketobutyrate production by alpha,gamma-elimination of l-methionine catalyzed by l-methionine gamma-lyase is not very feasible, because the enzyme simultaneously catalyzes both gamma-replacement and alpha,gamma-elimination. to develop an accurate enzyme assay, the comprehensive enzyme kinetics needed to be elucidated by progress curve analysis on the basis o ...200415051540
serological characterization of the o-specific polysaccharide of providencia alcalifaciens o23.the genus providencia belongs to the enterobacteriaceae family and currently consists of five species: p. alcalifaciens, p. heimbachae, p. rettgerii, p. rustigianii and p. stuartii. the serological classification scheme of p. alcalifaciens, p. rustigianii and p. stuartii includes 63 o-serogroups and 30 h-serogroups. the o-antigenic specificity is defined by the structure of the o-antigen (o-specific polysaccharide--ops), a part of the lipopolysaccharide (lps, endotoxin), one of the major compone ...200415053232
comparative study of the in vitro activity of a new fluoroquinolone, abt-492.the in vitro activity of a new fluoroquinolone, abt-492, was determined.200415056651
possible occurrence of a crabtree effect in the production of lactic and butyric acids by a floc-forming bacterial consortium.lactic and butyric acid production by bacterial flocs in a continuous culture obeyed different physiological constraints. the butyric acid rate of production was constant and independent of the growth rate [0.012 +/- 0.001 gbut/(l.h)], whereas lactic fermentation occurred only beyond a critical growth rate (0.25 +/- 0.05 h(-1)) and was apparently associated with an abrupt drop in biomass. principles of modeling used to describe a crabtree effect in saccharomyces cerevisiae were found to apply to ...200415057470
island nail transfer in the treatment of macrodactyly of the great toe: a case report.macrodactyly involving the great toe is difficult to treat when both the functional and cosmetic problems are addressed simultaneously. partial resection of the distal phalanx is an easy way to reduce the length of the involved toe. however, there are cosmetic concerns with loss of the toenail. the authors report on a clinical case in which shortening and debulking of the toe was performed while preserving the hallux nail. the nail complex was transferred as an island flap to the dorsal aspect o ...200415057859
effects of pnpg on cell growth cycle, motility machinery and quorum sensing in serratia marcescens.p-nitrophenylglycerol (pnpg) effectively inhibits swarming of the enterobacterium proteus mirabilis. the underlying mechanism of inhibition is unclear. we have now found that both pnpg also inhibits motility and swarming in another enterobacterium, serratia marcescens. while the peak promoter activities of the flagellar master operon (flhdcsm), the flagellin structural gene (hagsm) and the nuclease gene (nucasm) in s. marcescens increased with increasing pnpg concentration, the expression of the ...200415060680
maxillary sinus disease of odontogenic origin.odontogenic sinusitis is a well-recognized condition and accounts for approximately 10% to 12% of cases of maxillary sinusitis. an odontogenic source should be considered in patients with symptoms of maxillary sinusitis who give a history positive for odontogenic infection or dentoalveolar surgery or who are resistant to standard sinusitis therapy. diagnosis usually requires a thorough dental and clinical evaluation with appropriate radiographs. common causes of odontogenic sinusitis include den ...200415064067
[a case report of douglas abscess due to diverticulitis of the sigmoid colon associated with liver abscess]. 200415065348
histology and ultrastructure of the gut epithelium of the neotenic cave salamander, proteus anguinus (amphibia, caudata).histological, histochemical, and ultrastructural features of the gut of the european endemic cave salamander proteus anguinus were studied. the gut is a relatively undifferentiated muscular tube lined with a simple columnar epithelium containing numerous goblet cells. the mucosa and underlying lamina propria/submucosa are elevated into a number of high longitudinal folds projecting into the lumen. the enterocytes are covered apically with uniform microvilli. irregularity in the arrangement of mi ...200414666527
phylogenetic analysis and in situ identification of the intestinal microbial community of rainbow trout (oncorhynchus mykiss, walbaum).to identify the dominant culturable and nonculturable microbiota of rainbow trout intestine.200414678165
evaluation of novel beta-ribosidase substrates for the differentiation of gram-negative bacteria.to synthesize novel substrates for the detection of beta-ribosidase and assess their potential for the differentiation of gram-negative bacteria.200414678171
growth phase-dependent regulation and stringent control of fis are conserved processes in enteric bacteria and involve a single promoter (fis p) in escherichia coli.the intracellular concentration of the escherichia coli factor for inversion stimulation (fis), a global regulator of transcription and a facilitator of certain site-specific dna recombination events, varies substantially in response to changes in the nutritional environment and growth phase. under conditions of nutritional upshift, fis is transiently expressed at very high levels, whereas under induced starvation conditions, fis is repressed by stringent control. we show that both of these regu ...200414679232
development of an intranasal vaccine to prevent urinary tract infection by proteus mirabilis.proteus mirabilis commonly infects the complicated urinary tract and is associated with urolithiasis. stone formation is caused by bacterial urease, which hydrolyzes urea to ammonia, causing local ph to rise, and leads to the subsequent precipitation of magnesium ammonium phosphate (struvite) and calcium phosphate (apatite) crystals. to prevent these infections, we vaccinated cba mice with formalin-killed bacteria or purified mannose-resistant, proteus-like (mr/p) fimbriae, a surface antigen exp ...200414688082
an in vitro study of the anti-microbial efficacy of a 1% silver sulphadiazine and 0.2% chlorhexidine digluconate cream, 1% silver sulphadiazine cream and a silver coated dressing.burn sepsis is a leading cause of mortality and morbidity in patients with major burns. the use of topical anti-microbial agents has helped improve the survival in these patients. there are a number of anti-microbials available, one of which, silvazine (1% silver sulphadiazine (ssd) and 0.2% chlorhexidine digluconate), is used only in australasia. no study, in vitro or clinical, had compared silvazine with the new dressing acticoat. this study compared the anti-microbial activity of silvazine, a ...200414693084
infections in diabetic burn patients.diabetic burn patients comprise a significant population in burn centers. the purpose of the study was to determine the demographic characteristics of diabetic burn patients and their rate of community-acquired and nosocomial infections.200414693994
structure and cross-reactivity of the o-antigen of providencia stuartii o18 containing 3-acetamido-3,6-dideoxy-d-glucose.the o-polysaccharide (o-antigen) of providencia stuartii o18 was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods and nmr spectroscopy, including 2d 1h,1h cosy, tocsy, noesy and 1h,13c hsqc experiments. the following structure of the tetrasaccharide repeating unit of the polysaccharide was established: [structure: see text] where qui3nac is 3-acetamido-3,6-dideoxyglucose. anti-p. stuartii o18 serum cross-reacted with the o-antigen of proteus genomospeci ...200414698900
[impact of secondary pancreatic infection on the prognosis of patients with severe acute pancreatitis: an analysis of 60 cases].to investigate the impact of secondary pancreatic infection (spi) on the prognosis of patients with severe acute pancreatitis (sap).200414706191
gene switching in amoeba proteus caused by endosymbiotic bacteria.the expression of genes for s-adenosylmethionine synthetase (sams), which catalyzes the synthesis of s-adenosylmethionine (adomet), a major methyl donor in cells, was studied in symbiont-free (d) and symbiont-bearing (xd) amoeba strains to determine the effect of bacterial endosymbionts. the symbionts suppressed the expression of the gene in host xd amoebae, but amoebae still exhibited about half the enzyme activity found in symbiont-free d amoebae. the study was aimed at elucidating mechanisms ...200414709722
adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering.mean macromolecular dynamics was quantified in vivo by neutron scattering in psychrophile, mesophile, thermophile and hyperthermophile bacteria. root mean square atomic fluctuation amplitudes determining macromolecular flexibility were found to be similar for each organism at its physiological temperature ( approximately 1 a in the 0.1 ns timescale). effective force constants determining the mean macromolecular resilience were found to increase with physiological temperature from 0.2 n/m for the ...200414710189
cephalosporin resistant urinary tract infections in young children.to describe the antibiotic resistance pattern of bacteria causing urinary tract infection (uti) in a cohort of australian children under 6 years of age.200414718005
sir frederick treves and sympathetic ophthalmia. 200414718303
in vivo expression of the mannose-resistant fimbriae of photorhabdus temperata k122 during insect infection.photorhabdus temperata k122 is an entomopathogenic bacterium symbiotically associated with nematodes of the family heterorhabditidae: surface fimbriae are important for the colonization of many pathogenic bacteria, and here we report the nucleotide sequence and analysis of the expression of a 12-kbp fragment encoding the mannose-resistant fimbriae of p. temperata (mrf). the mrf gene cluster contains 11 genes with an organization similar to that of the mrp locus from proteus mirabilis. mrfi (enco ...200414729685
results from the meropenem yearly susceptibility test information collection (mystic) programme: report of the 2001 data from 15 united states medical centres.the meropenem yearly susceptibility test information collection (mystic) programme is an international surveillance network of more than 100 medical centres where meropenem is the primary therapeutic carbapenem. institutions have been monitored since 1997 (1999 in united states (us)) using national committee for clinical laboratory standards (nccls) reference susceptibility methods to monitor in vitro activity of meropenem and selected other broad-spectrum antimicrobial agents. in 2001, a total ...200414732314
replacement of broad-spectrum cephalosporins by piperacillin-tazobactam: impact on sustained high rates of bacterial resistance.we have previously observed a significant reduction of ceftriaxone resistance in proteus mirabilis associated with an increase in the use of cefepime, along with a decrease in the consumption of broad-spectrum cephalosporins (cep). however, we did not observe such a reduction with klebsiella pneumoniae. therefore, we sought to determine whether replacement of cep by piperacillin-tazobactam might be useful in reducing sustained high rates of cep resistance by this organism. we used a 6-month "bef ...200414742186
emergence of methicilllin resistant staphylococcus aureus (mrsa) associated with wound infections.in a hospital setting antimicrobial resistant organisms especially methicillin resistant staphylococcus aureus (mrsa) has emerged as an important variable influencing patients' outcome and overall resource utilisation. the present study was undertaken to find out the proportion of mrsa and other organisms and their antimicrobial resistance pattern in admitted cases with postoperative wound infections. a total of 50 wound swabs were collected irrespective of age and sex of the patients from natio ...200414747792
evidence that putrescine acts as an extracellular signal required for swarming in proteus mirabilis.in a search for proteus mirabilis genes that were regulated by cell-to-cell signalling, a lacz fusion (cmr437::mini-tn5lacz) was identified that was repressed 10-fold by a self-produced extracellular signal from wild-type cells. however, the cmr437::mini-tn5lacz insertion itself led to a marked reduction in this extracellular repressing signal. the cmr437::mini-tn5lacz insertion was mapped to a spea homologue in p. mirabilis. sequence analysis indicated that a speb homologue was encoded downstre ...200414756784
bacterial colonization of patients undergoing international air transport: a prospective epidemiologic study.to investigate the incidence of multiresistant bacteria in patients treated in foreign hospitals and repatriated by international interhospital air transport.200414769287
roxithromycin antagonism with essential and trace elements.in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various macrolide antibiotics, the synergistic or antagonistic behavior of roxithromycin metal complexes have been studied and compared with the parent drug. metal complexes of roxithromycin with magnesium, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc and cadmium have been investigated for their antibacterial activity and compared with roxithromycin by observing ...200416414588
urinary tract infections associated with multidrug resistant enteric bacilli: characterization and genetical studies.urinary tract infections (utis) are among the most commonly prevalent infections in clinical practice. escherichia coli is the causative agent in about 85% of community-acquired utis, followed by klebsiella that accounts for 6 to 17% of such infections. present study is based on the isolation-identification and antibiotic resistance pattern of about 60 indigenous bacterial isolates from uti patients. prevalence rates were consistent with those from major recent studies reported in the literature ...200416414603
the susceptibility of certain bacterial strains to imidazotriazine derivatives.the synthesized imidazo[2,1-c]triazines were tested for their antimicrobial activity. microbiological screening tests conducted in relation to 54 strains of gram-positive and 52 strains of gram-negative bacteria, 6 strains of yeast-like fungi and 3 strains of moulds showed significant activity of compound 19 against 52 gram-negative bacterial strains. this compound was active against 16 strains of escherichia coli, 12 strains of pseudomonas, 10 strains of proteus spp., 8 strains of klebsiella pn ...200416146057
recurrent urinary tract infections--uropathogen incidence and drug sensitivity.the aim of the study was the retrospective analysis of the results of the drug sensitivity studies of bacterial species cultured from 681 urinary specimens collected from 79 children (12 boys and 67 girls) aged 0-18 years with recurrent urinary tract infection in a period from 1997 to 2001. factors predisposing to recurrent urinary tract infections and the incidences of particular uropathogens were also analyzed. escherichia coli (57.3%), proteus spp. (15.6%) and pseudomonas aeruginosa (9.1%) we ...200416146092
synthesis, spectra and biomimetic properties of copper(ii)-copper(ii) and copper(ii)-zinc(ii) binuclear complexes with cun5 chromophores.x-band electron spin resonance (esr) and uv-vis spectra of a homobinuclear [(bipy)2cu-e-im-cu(bipy)2](bf4)3 and a heterobinuclear [(bipy)2cu-e-im-zn(bipy)2](bf4)3 complexes, e-im=2-ethylimidazolate ion have been described as possible models for superoxide dismutase (sod). magnetic moment and esr spectral measurements of the homobinuclear complex have shown an antiferromagnetic exchange interaction. from ph-dependent esr and uv-vis spectral measurements studies, these complexes have been found to ...200416043054
oropharyngeal flora changes in patients with head and neck malignancy post radiotherapy.radiotherapy has been recognized as a valuable modality of treatment in the management of head and neck cancers. it can have a direct bactericidal effect on the normal flora of the oropharynx. the objective of this study is to determine the changes in the oropharyngeal flora after external beam radiation. this prospective non randomized control study was performed to aid in identification of organisms involved in sepsis, as well as aid in choosing appropriate antibiotics for surgical procedures ...200415889559
microwave irradiation for shortening the processing time of samples of flagellated bacteria for scanning electron microscopy.microwave irradiation (mwi) has been applied to the development of rapid methods to process biological samples for scanning electron microscopy (sem). in this paper we propose two simple and quick techniques for processing bacteria (proteus mirabilis and vibrio mimicus) for sem using mwi. in the simplest methodology, the bacteria were placed on a cover-glass, air-dried, and submitted to conductivity stain. the reagent used for the conductivity stain was the mordant of a light microscopy staining ...200417061527
bacteriological quality of some pharmaceutical products marketed by drug vendors in uyo, nigeria.bacteriological quality of some pharmaceutical products purchased from open markets, buses and drug stores in uyo metropolis was studied in order to determine the level of contamination of the drugs. the drug samples examined were tetracycline capsules, paracetamol tablets, ampicillin capsules, chloroquine tablets, chloroquine syrup, chloroquine injections, flagyl tablets and chloramphenicol tablets. the bacterial count ranged from 2.3x10(4) cfu/g to 4.4x10(4) cfu/g for the 16 samples of ampicil ...200417298130
evaluation of the antimicrobial activity of the acetone extract of the lichen ramalina farinacea and its (+)-usnic acid, norstictic acid, and protocetraric acid constituents.the acetone extract of the lichen ramalina farinacea and its (+)-usnic acid constituent showed antimicrobial activity against bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocolitica, candida albicans, and candida glabrata. norstictic acid was active against aeromonas hydrophila as well as the above microorganisms except yersinia enterocolitica. protocetraric acid showed activity only against the tested yeasts candida alb ...200418998406
bacteriology of diabetic foot lesions.clinical grading and bacteriological study of 107 patients with diabetic foot lesions revealed polymicrobial aetiology in 69 (64.4%) and single aetiology in 21 (19.6%). among 107 patients 62 had ulcer. of these 31 had mixed aerobes. twenty six patients with cellulitis and 12 with gangrene had more than 5 types of aerobes and anaerobes such as e. coli, klebsiella spp., pseudomonas spp., proteus spp., enterobactor spp., enterococci spp., clostridium perfringens, bacteroides spp., prevotella spp. a ...200417642727
depression - the proteus of medicine.depression is discussed as a disease of antiquity with suitable contemporary references also. the prevalence of this disorder, which at a given time constitutes 121 millions world-wide is mentioned. among the types of depression, comorbid depression forms an important one. classical depression forms the visible part of the depression iceberg while somatic and other life contextual situation forms the submerged part. somatic manifestations per se do not carry diagnostic weightage unless the core ...200421408045
a rapid method for determining mycobacterium tuberculosis based on a bulk acoustic wave impedance biosensor.the bulk acoustic wave impedance biosensor was set up and used to monitor the growth of mycobacterium tuberculosis (m.tb). this sensor is rapid, simple, sensitive (lower limit is 2 x 10(3) cells ml(-1)) and cheap (easy to generalize). the typical response curve was different from other bacteria's, such as escherichia coli, staphylococcus aureus, proteus mirabilis. the frequency detection time was used to quantitatively determine m.tb. it was proportional to logarithm of the initial concentration ...200318968982
polymicrobial osteomyelitis of the odontoid process with epidural abscess: case report and review of literature.osteomyelitis of the odontoid process is a rare disease and staphylococcus aureus is the usual causative organism. we present a case caused by a synergistic infection with staphylococcus aureus and proteus mirabilis, which has not been reported previously. a high index of suspicion is required for diagnosis, and early institution of broad antimicrobial therapy, including agents active against gram-negative organisms, is mandatory.200315912167
[catheter-related urinary infections at the clinical center in banja luka].catheter-associated urinary tract infections are the most common nosocomial infections of the urinary tract, and among the most common nosocomial infections in general. the major problems of these infections include antibiotic resistance and enormous direct and indirect cost of treatment.200314740538
the antimicrobial activity of extracts of the lichen cetraria aculeata and its protolichesterinic acid constituent.in this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen cetraria aculeata has been investigated. the extracts were tested against twelve bacteria and eight fungi and found active against escherichia coli, staphylococcus aureus, aeromonas hydrophila, proteus vulgaris, streptococcus faecalis, bacillus cereus, bacillus subtilis, pseudomonas aeruginosa, listeria monocytogenes. no antimicrobial activity against the fungi was detected. it was determin ...200314713164
characteristics of trajectory in the migration of amoeba proteus.we investigated the behavior of migration of amoeba proteus in an isotropic environment. we found that the trajectory in the migration of a. proteus is smooth in the observation time of 500-1000 s, but its migration every second (the cell velocity) on the trajectory randomly changes. stochastic analysis of the cell velocity and the turn angle of the trajectory has shown that the histograms of the both variables well fit to gaussian curves. supposing a simple model equation for the cell motion, w ...200314714206
prevalence of bacterial pathogens in infected wounds in a tertiary hospital, 1995-2001: any change in trend?wound care is a very important aspect of surgical care. knowledge of the epidemiology of bacterial pathogens associated with infected wounds is critical in formulating policies on infection control.200314717475
septicemia caused by retained pacemaker lead tip. 200314693331
relationship between fluoroquinolone use and changes in susceptibility to fluoroquinolones of selected pathogens in 10 united states teaching hospitals, 1991-2000.we retrospectively examined the relationship between fluoroquinolone use and the susceptibilities of 11 bacterial pathogens to fluoroquinolones in 10 us teaching hospitals from 1991 through 2000. statistical significance was determined by 2-way analysis of variance, with the number of isolates tested each year as a weighting factor. the analysis of baseline-to-end point change in the percentage of susceptibility and the slope of the regression line (trend line) for logit percentage of susceptibi ...200314689346
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2001). i. susceptibility distribution].the bacterial strains isolated from patients diagnosed as having urinary tract infections (utis) in 10 institutions in japan were supplied between the period of september and december 2001. then, the susceptibilities of them to a variety of antimicrobial agents were investigated. the number of them were 496 strains. the breakdown of these strains was gram-positive bacteria as 29.6% and gram-negative bacteria as 70.4%. susceptibilities of these bacteria to antimicrobial agents were as follows; ag ...200314692378
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, monobactams, and penicillins. changes in czop susceptibility among bacteria were also evaluated with the bacterial resistance ratio calculated from the breakpoint mic. twenty-five species (4,154 strains) of gram-negative bacteria were isolated from the clinical ...200314692381
structural studies on the lipopolysaccharide core of proteus ox strains used in weil-felix test: a mass spectrometric approach.the core region of the lipopolysaccharides of proteus group ox bacteria, which are used as antigens in weil-felix test for serodiagnosis of rickettsiosis, were studied by chemical degradations in combination with esi ftms, including infrared multi-photon dissociation (irmpd) ms/ms and capillary skimmer dissociation. structural variants of the inner core region were found to be the same as in proteus non-ox strains that have been studied earlier. the outer core region has essentially the same str ...200314670728
swarming of proteus mirabilis over ureteral stents: a comparative assessment.encrustation on indwelling ureteral stents is commonly related to the presence of urease-producing bacteria that elevate the ph of the urine through the hydrolysis of urea, resulting in the precipitation of calcium and magnesium salts. using a model previously shown to measure accurately the ability of proteus mirabilis to swarm over catheter surfaces (eur j clin microbiol infect dis 1999;18:206), we investigated the ability of this organism to swarm over three ureteral stents with potential enc ...200314565887
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2001--ii. gram-negative bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2001 were yearly evaluated and compared with those of other cephems, oxacephems and carbapenems. a total of 3,245 strains in 32 species of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis, escherichia coli, citrobacter freundii, citrobact ...200314567255
bacteriology of chronic suppurative otitis media in congolese children.the study intended to identify bacteria active in the chronic suppurative otitis media and to determine their sensitivity to current antibiotics.200314571655
development of a pcr assay for detection of enterobacteriaceae in foods.a broad-range pcr assay for the detection of bacteria belonging to the enterobacteriaceae family was developed in this study. primers targeting the bacterial 16s rrna gene were newly designed and used in this pcr assay. to determine the specificity of the assay, 72 different bacterial species (of 49 genera), 2 fungi, 3 animals, and 4 plants were tested. results were positive for every tested vibrioaceae or enterobacteriaceae strain except proteus mirabilis. for all other bacterial strains and eu ...200314572216
susceptibility trends in bacteraemias: analyses of 7544 patient-unique bacteraemic episodes spanning 11 years (1990-2000).the aim of the present study was to design more accurate tools for the selection of appropriate antimicrobial therapy for hospitalized patients with suspected sepsis. we created a large database comprising data on all patient-unique blood cultures obtained over an 11 year period (1 january, 1990 through 31 december, 2000). improved statistical tools were applied to assess the trends in in vitro activity of individual antibiotic agents against various bacteria over time, and to calculate suscepti ...200314572487
comparison of two test methods for the determination of sufficient antimicrobial activity of three commonly used alcohol-based hand rubs for hygienic hand disinfection.in europe, the antimicrobial efficacy of alcohol-based hand rubs is determined with a quantitative suspension test (pren 12054) and a test under practical conditions (en 1500). another test method has recently been published by the german society for hygiene and microbiology (dghm) with four differences to the european system in the in vitro tests: additional qualitative suspension tests with product dilutions to the ineffective range; a selection of the most resistant gram-negative test strains ...200314572490
structure of the o-polysaccharide of proteus mirabilis o38 containing 2-acetamidoethyl phosphate and n-linked d-aspartic acid.the o-antigen of proteus mirabilis o38 was found to be unique among bacterial polysaccharides and to have the following structure: [carbohydrate structure in text] where d-qui4n(ac-d-asp) is 4-(n-acetyl-d-aspart-4-ylamino)-4,6-dideoxy-d-glucose and acetnp is 2-acetamidoethyl phosphate. neither of these entities have been hitherto found in natural polysaccharides. structural studies were performed using 1d and 2d nmr spectroscopy, including experiments run in an h2o/d2o mixture to reveal correlat ...200314572723
why are foley catheters so vulnerable to encrustation and blockage by crystalline bacterial biofilm?many patients undergoing long-term bladder catheterisation experience blockage and encrustation of their catheters. the problem stems from infection by urease producing bacteria, particularly proteus mirabilis. bacterial biofilms colonise the catheters, the activity of urease raises the ph and induces the deposition of calcium and magnesium phosphate crystals. in this study, a laboratory model of the catheterised bladder has been used to examine the early stages in the formation of the crystalli ...200314574534
extended-spectrum beta-lactamase-producing enterobacteriaceae in community and private health care centers.in 1999, 39 of 2,599 isolates of the family enterobacteriaceae (1.5%) collected by eight private laboratories in the aquitaine region in france produced an extended-spectrum beta-lactamase (esbl). among these were 19 enterobacter aerogenes isolates; 8 klebsiella pneumoniae isolates; 6 escherichia coli isolates; 3 proteus mirabilis isolates; and 1 isolate each of serratia marcescens, morganella morganii, and providencia stuartii. esbl producers were isolated from 38 patients, including 33 residen ...200314576109
[urinary tract infection in institutionalized elderly patients. incidence of bacterial resistance and risk factors].the goal of the present work was to study urinary tract infections (uti) in a sociosanitary center in order to characterize etiology, define sensitivity profiles, and study associations between these profiles and various clinical parameters.200314576919
proteus mirabilis and rheumatoid arthritis: no association with the disease.proteus mirabilis (pm) is implicated in different studies in the pathogenesis of rheumatoid arthritis (ra) because of the structural homogeneity of its haemolysin b precursor with eqrraa sequences in drb 1 haplotype. the aim of the study was to compare the levels of antibodies specific to pm in the sera of patients with ra and healthy controls in our population. serum samples from 78 consecutive ra patients and 75 healthy controls were analysed for the presence of igg isotype and total immunoglo ...200314576988
[antibacterial effect of nitric oxide].in this study, the antibacterial effects of nitric oxide (no) have been investigated against 4 strains of multidrug-resistant mycobacterium tuberculosis and 8 klebsiella, 7 escherichia coli, 7 staphylococcus, 5 enterobacter, 7 pseudomonas and 7 proteus clinical isolates by using deta-no ((z)-1-[n-(2-aminoethyl)-n-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate) as the no donor. minimum inhibitory concentrations (mics) were determined by broth microdilution method for all tested microorganisms. th ...200314593897
composition and antimicrobial activity of achillea clavennae l. essential oil.the volatile constituents of achillea clavennae l. (asteraceae), rare plant of europe, have been analysed using gc/ms. twenty- five components making up 81.6% of the oil were characterized with camphor (29.5%), myrcene (5.5%), 1,8-cineole (5.3%), beta-caryophyllene (5.1%) and linalool (4.9%) being the major constituents. the essential oil was evaluated for antibacterial and antifungal activities. the screening of the antimicrobial activity of essential oil was conducted by a disc diffusion test ...200314595583
argentinean collaborative multicenter study on the in vitro comparative activity of piperacillin-tazobactam against selected bacterial isolates recovered from hospitalized patients.the in vitro activity of piperacillin-tazobactam and several antibacterial drugs commonly used in argentinean hospitals for the treatment of severe infections was determined against selected but consecutively isolated strains from clinical specimens recovered from hospitalized patients at 17 different hospitals from 9 argentinean cities from different geographic areas during the period november 2001-march 2002. out of 418 enterobacteriaceae included in the study 84% were susceptible to piperacil ...200314596972
proteus mirabilis fimbriae (pmf) are important for both bladder and kidney colonization in mice.proteus mirabilis expresses different types of fimbriae simultaneously. several fimbrial types have been described and their role in the colonization of the urinary tract is under study. previously, p. mirabilis fimbriae (pmf) have been shown to be associated with bacterial colonization of the lower urinary tract but not of the kidneys. in this study, a pmfa mutant was generated and used in several in vivo and in vitro studies. two different urinary tract infection models in the mouse and two in ...200314600235
evolution of aminoglycoside resistance phenotypes of four gram-negative bacteria: an 8-year survey in a university hospital in greece.in order to determine the resistance patterns and evolution trends of four common enterobacteriaceae (escherichia coli, proteus spp., klebsiella spp. and enterobacter spp.), aminoglycoside resistance phenotypes of 8917 non-repetitive strains, isolated over an 8-year period, were analysed. phenotypes were defined by examining the susceptibility of the strains to a panel of aminoglycosides, using disk diffusion method. a large diversity of different resistance phenotypes was encountered. a signifi ...200314602373
molecular epidemiology of proteus mirabilis infections of the catheterized urinary tract.proteus mirabilis compromises the care of many patients undergoing long-term indwelling bladder catheterization. it forms crystalline bacterial biofilms in catheters which block the flow of urine, causing either incontinence due to leakage or painful distention of the bladder due to urinary retention. if it is not dealt with, catheter blockage can lead to pyelonephritis and septicemia. we have examined the epidemiology of catheter-associated p. mirabilis infections by use of pulsed-field gel ele ...200314605124
soil microbial populations, community composition, and activity as affected by repeated applications of hog and cattle manure in eastern saskatchewan.a field site near humboldt, saskatchewan, was annually treated with hog or cattle manure and cropped to canola, spring wheat, barley, and canola from 1997 to 2000. during each growing season, soil was analyzed for microbial populations in terms of activity and community structure, and crops were assessed for root rot and foliar diseases. microbial activity in soils treated with cattle manure was higher than in soils treated with hog manure or urea. similarly, nitrous oxide emissions from soil in ...200314608420
comparative evaluation of three chromogenic agars for detection and rapid identification of aerobic gram-negative bacteria in the normal intestinal microflora.to compare three different chromogenic agars and macconkey agar for the detection of aerobic gram-negative bacteria in the normal intestinal microflora and to assess the accuracy of the chromogenic agars for the direct identification of escherichia coli.200314616678
comparative in vitro activity of piperacillin, piperacillin-sulbactam and piperacillin-tazobactam against nosocomial pathogens isolated from intensive care patients.we investigated the antimicrobial activity of piperacillin-tazobactam versus piperacillin-sulbactam against common nosocomial pathogens (n = 565) isolated from intensive care patients. for gram-positive bacteria, antimicrobial susceptibilities to the two piperacillin-beta-lactamase inhibitor combinations were almost identical. for gram-negative bacteria, piperacillin-tazobactam exhibited greater activity against escherichia coli and proteus vulgaris than piperacillin-sulbactam. both combinations ...200314616732
[antibacterial activity of pure cultures of cyanobacteria and algae].pure cultures of microcystis aeruginosa, platymonas viridis and nephrochloris salina have been grown on the media with different nitrogen and phosphorus content. their supernatants and pellets, as well as lipid complex, terpene fraction and some its components from m. aeruginosa had selective antibacterial characteristics. the increase of nitrogen content in the medium correlated with the intensification (m. aeruginosa, n. salina) or conservation (p. viridis) of bactericidal activity. the pellet ...200314618789
bioassay screening of amazonian plants.the purpose of this study was to evaluate several biological activities of thirty plant extracts collected in the north west amazon (ecuador). some of these plants are being used for their reputed medicinal properties by the natives of this region.200314619457
separation of glutathione transferase subunits from proteus vulgaris by two-dimensional gel electrophoresis.cytosolic glutathione transferases of proteus vulgaris were purified by affinity chromatography and characterized by two-dimensional gel electrophoresis. four different subunits were identified, and each subunit contained a different molecular mass, ranging from 26.2 kda to 28.5 kda; a different pi value, ranging from 8.2 to 9.4; and a different amount of protein fraction, ranging from 10% to 56%. all four subunits existed as basic proteins (pi > 7.0). from these results, we concluded that multi ...200314629019
antimicrobial activity of gymnema sylvestre leaf extract.the ethanolic extract of gymnema sylvestre leaves demonstrated antimicrobial activity against bacillus pumilis, b. subtilis, pseudomonas aeruginosa and staphylococcus aureus and inactivity against proteus vulgaris and escherichia coli.200314630178
vesical calculi with unrepaired vesicovaginal fistula: a clinical appraisal of an uncommon association.primary vesical calculi are uncommon in patients with vesicovaginal fistula (vvf). we retrospectively analyzed 19 such cases and present our experience with the management of this condition.200314634380
the proteus syndrome associated with life threatening hematuria. 200314634442
role of arginine 226 in the mechanism of tryptophan indole-lyase from proteus vulgaris.in the spatial structure of tryptophanase from proteus vulgaris the guanidinium group of arginine 226 forms a salt bridge with the 3;-oxygen atom of the coenzyme. the replacement of arginine 226 with alanine using site-directed mutagenesis reduced the affinity of the coenzyme for the protein by one order of magnitude compared to the wild-type enzyme. the catalytic activity of the mutant enzyme in the reaction with l-tryptophan was reduced 10(5)-fold compared to the wild-type enzyme. the rates of ...200314640959
polyhalogenated benzo- and naphthoquinones are potent inhibitors of plant and bacterial ureases.polyhalogenated benzo- and naphthoquinones were found to be potent inhibitors of pure ureases from bacillus pasteurii and canavalia ensiformis. they also inhibited ureases in whole cells of helicobacter pylori, klebsiella oxytoca and proteus mirabilis. inhibition was non-competitive with k(i) values in the micromolar range or below. inhibition was irreversible as shown by equilibrium dialysis. inhibitory power decreased considerably when halogens were replaced by -oh, -cn, alkoxy or alkyl groups ...200314644444
structural studies of proteus mirabilis catalase in its ground state, oxidized state and in complex with formic acid.the structure of proteus mirabilis catalase in complex with an inhibitor, formic acid, has been solved at 2.3 a resolution. formic acid is a key ligand of catalase because of its ability to react with the ferric enzyme, giving a high-spin iron complex. alternatively, it can react with two transient oxidized intermediates of the enzymatic mechanism, compounds i and ii. in this work, the structures of native p. mirabilis catalase (pmc) and compound i have also been determined at high resolution (2 ...200314646074
endoscopic treatment in a patient with obstructive jaundice caused by pancreatic pseudocyst.we report a case of chronic alcoholic pancreatitis with obstructive jaundice due to compression of the common bile duct by pancreatic pseudocyst. ultrasonography and computed tomographic scan on admission demonstrated an 8 cm cystic lesion located at the head of the pancreas. endoscopic retrograde cholangiopancreatography (ercp) revealed a segment of narrowing at the distal common bile duct which was compressed by the pancreatic pseudocyst. communication between the cyst and pancreatic duct was ...200314649681
proteus syndrome.proteus syndrome is a rare sporadic, hamartoneoplastic disorder of vascular, skeletal, and soft tissues that causes asymmetry of the skull, body, arms, and the legs. the name "proteus" of the greek god who had the ability to change his shape was coined to define the variety of deformities including partial gigantism of the hands or feet, asymmetry of the arms and legs, hypertrophy of long bones, plantar hyperplasia, haemangiomas, lipomas, varicosities, linear verrucous epidermal naevi, macroceph ...200314649692
norfloxacin-releasing urethral catheter for long-term catheterization.norfloxacin-releasing urethral catheters were prepared for the purpose of preventing urinary tract infections during long-term catheterization. the outer and inner surfaces of the catheters were coated with poly(ethylene-co-vinyl acetate) (eva) and an amphiphilic multiblock co-polymer (peo2kpdms), composed of poly(ethylene oxide) and poly(dimethyl siloxane). norfloxacin, a fluoroquinolone synthetic antibiotic, was impregnated into a coating layer. the in vitro drug release behavior was monitored ...200314661872
gentamicin-releasing urethral catheter for short-term catheterization.urethral catheters, widely used for the drainage of the bladder, are associated with most urinary tract infections (utis) that account for 40% of all episodes occurring in acute-care hospitals. this study aimed to develop a gentamicin-releasing catheter that effectively prevents utis for short-term catheterization. for physical loading of gentamicin, the urethral catheters were coated by the simple dipping method with poly(ethylene-co-vinyl acetate) (eva) and eva/poly(ethylene oxide) (peo) blend ...200314661873
nosocomial outbreak of infections by proteus mirabilis that produces extended-spectrum ctx-m-2 type beta-lactamase.nineteen multidrug-resistant proteus mirabilis strains were isolated from 19 patients suffering from infections probably caused by p. mirabilis. these strains were recovered from urine or other urogenital specimens of 16 inpatients and three outpatients with a hospitalization history in a urology ward of funabashi medical center, from july 2001 to august 2002. these strains demonstrated resistance to cefotaxime, ceftriaxone, cefpodoxime, and aztreonam, while they were highly susceptible to cefta ...200314662935
differential regulation of the proteus mirabilis urease gene cluster by urer and h-ns.proteus mirabilis, a cause of catheter-associated urinary tract infection, relies on several virulence factors to colonize the urinary tract. among these, urease contributes to the development of urinary stones resulting from the increase in local ph due to urease-mediated hydrolysis of urea to nh(3) and co(2). urer, an arac-like transcriptional activator, activates transcription of the genes encoding the urease subunits and accessory proteins (uredabcefg) in the presence of urea. urer also init ...200314663072
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