TitleAbstractYear(sorted descending)
incidence of extended-spectrum beta-lactamases in clinical isolates of the family enterobacteriaceae in a pediatric hospital.the incidence of extended-spectrum beta-lactamases (esbls) was analyzed in enterobacteriaceae population circulating in the upper silesian child and mother health center in katowice (usc&mhc). altogether 1164 clinical specimens, collected from children hospitalized in 8 different hospital units of usc&mhc were investigated. five hundred and eighty-five clinical isolates of the family enterobacteriaceae were identified in specimens collected from 403 patients. two hundred and twenty-nine enteroba ...200415330264
per-1 production in a urinary isolate of providencia rettgeri.a providencia rettgeri strain resistant to extended-spectrum cephalosporins and intermediate to aztreonam was isolated from the urine of a patient hospitalized in the urology clinic of ssk educational hospital in ankara. clavulanic acid restored the activity of extended-spectrum cephalosporins, suggesting that the strain was harboring an extended-spectrum beta-lactamase. since the per-1 enzyme is widespread in turkey, and had been already detected in a related species such as proteus mirabilis, ...200415332708
[synthesis and antibacterial activity of 2-(3-pyridyl)-5-([(5-aryl-1,3,4-oxadiazol-2-yl) methylene]thio)-1,3,4-oxadiazoles].studies on synthesis and antibacterial activity of new heterocycles.200415303654
treatment of dairy wastewater using a selected bacterial isolate, alcaligenes sp. mmrr7.physicochemical and biologic analysis of dairy wastewater showed that the effluent had a high organic load (chemical oxygen demand [cod]: 5095 mg/l), an acidic ph (6.4), and a high probability of coliforms (most probable number [mpn] >1100). the various bacterial strains isolated and purified were identified as sporolactobacillus sp., citrobacter sp., pseudomonas sp., alcaligenes sp., bacillus sp., staphylococcus sp., and proteus sp., as per the bergey's manual of systematic bacteriology. among ...200415304739
[bacterial infections in chronic otitis media--usefulness of molecular diagnostics based on pcr method].infections in chronic otitis media (com) are common, and identification of their pathogen is difficult, what is proven by high rate of negative results of bacteriological examinations. this fact may be explained by two factors: it is difficult to obtain a reliable material for culture and classic methods of bacteriological cultures may be of low sensitivity. amplification of bacterial dna in pcr is an examination, that leads to a higher effectiveness and sensitivity of bacteriological tests. the ...200415311593
cutaneous manifestations of proteus syndrome: correlations with general clinical severity.proteus syndrome is a rare congenital disorder with progressive asymetric overgrowth of multiple tissues.200415313810
the manifold faces of proteus syndrome. 200415313819
[molecular mechanisms of cephalosporin resistance in gram-negative bacteria of the enterobacteriaceae family].extended spectrum beta lactamase genes were detected by the pcr in 87.6% of 231 enterobacteriaceae strains isolated in medical institutions of moscow, st. petersburg, tomsk and nazran that showed a decrease in their susceptibility to 3rd generation cephalosporins. alone or in various combinations tem type beta lactamases were detected in 43.3% of the isolates, 46.8 and 51.2% of the isolates produced shv type and ctx type beta lactamases respectively. combinations of 2 and 3 different determinant ...200415344391
[use of therapeutic polycomponent vaccine "immunovac vp-4" for the treatment of chronic bacterial prostatitis].a complex of clinical and laboratory examinations of patients with bacterial chronic prostatitis was carried out. the patients were divided into two groups: 55 patients treated by the method of immunotherapy and 20 patients (controls) undergoing basic therapy. immunotherapy was carried out with the use of polycomponent vaccine vp-4 containing staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris and escherichia coli antigens. vaccinal therapy was indicated in cases with infection focus ...200415346948
transcription of proteus mirabilis flaab.proteus mirabilis, a gram-negative urinary tract pathogen, has two highly homologous, tandemly arranged flagellin-encoding genes, flaa and flab. flaa is transcribed from a sigma(28) promoter, while flab is a silent allele. previous studies have demonstrated the presence of a family of hybrid flagellin genes, referred to as flaab. these genes are composed of the 5' end of flaa and the 3' end of flab, and are produced through excision of the intervening dna between the two genes. although the exis ...200415347745
digestive pathology of sea turtles stranded in the canary islands between 1993 and 2001.digestive lesions were observed in 84 of 136 sea turtles (128 caretta caretta, four chelonia mydas and four dermochelys coriacea) stranded in the canary islands between january 1993 and december 2001. in the oral cavity ulcerative and necropurulent stomatitis were the most frequently observed lesions, and in the oesophagus ulcerative and fibrinous oesophagitis, and traumatic oesophageal perforation were most frequently observed; all these lesions were mainly associated with the ingestion of fish ...200415357377
poly(vinyl chloride)-coated sol-gels for studying the effects of nitric oxide release on bacterial adhesion.nitric oxide (no) releasing sol-gel materials coated with poly(vinyl chloride) (pvc) films exhibit increased stability at ambient and physiological temperatures. the polymer overcoat, however, reduces the no fluxes by 5-35% over the initial week of release. the variation in no fluxes between unmodified and pvc-coated sol-gels is negligible after 7 days. the pvc polymeric layer provides controlled surface chemistry for systematic studies of the effects of no release on bacterial adhesion. as an e ...200415360321
surgical infections: a microbiological study.surgical infections are mostly polymicrobial, involving both aerobes and anaerobes. one hundred seventeen cases comprised of abscesses (n=51), secondary peritonitis (n=25), necrotizing fascitis (n=22) and wounds with devitalized tissues (n=19) were studied. the number of microorganisms isolated per lesion was highest in secondary peritonitis (2.32). the aerobe/ anaerobe ratio was 0.81 in secondary peritonitis and 1.8 in necrotizing fascitis. most secondary peritonitis (80%), necrotizing fascitis ...200415361989
bacterial resistance to antimicrobials in urinary isolates.escherichia coli accounted for about 80% of organisms in uncomplicated urinary tract infections (utis), followed by staphylococcus spp. especially staphylococcus saprophyticus, and proteus mirabilis. against e. coli isolates from patients with uncomplicated uti, faropenem was the most effective. up to 1999, fluoroquinolone-resistant isolates were not observed in patients with uncomplicated uti, but in 2001 fluoroquinolone-resistant e. coli isolates emerged and accounted for about 8%. various typ ...200415364302
urinary tract infections in african infants.urinary tract infection (uti) causes significant illness in the first 2 years of life. the diagnosis in most developing countries is often overlooked due to the tedious nature of obtaining urine from these young ones who would not void voluntarily. misdiagnosis very often lead to avoidable ill health and long-term renal damage. there is a need to diagnose uti in febrile infants to alert clinicians. a prospective study of febrile infants aged up to 12 months on admission was undertaken in a 6-mon ...200415364303
the role of unusual pathogens in prostatitis syndrome.a total of 1442 patients with symptoms of chronic prostatitis were examined over a 4-year period at the outpatient department for urogenital infections, university hospital for infectious diseases "dr. fran mihaljevic", zagreb, croatia. an infectious aetiology was determined in 1070 (74.2%) patients. in 561 of 1070 (52.4%) patients the inflammatory finding (>10 wbc/hpf) was found in expressed prostatic secretions (eps) or voided bladder urine (vb(3)). normal, <10 wbcs/hpf was found in 362 of 536 ...200415364308
nosocomial transmission of ctx-m-2 beta-lactamase-producing acinetobacter baumannii in a neurosurgery ward.three strains of cefotaxime (ctx)-resistant acinetobacter baumannii, fm0209680, fm0300106, and fm0301433, were isolated from transtracheal aspirate cultures of three patients with probable nosocomial infections in a neurosurgery ward in japan. the ctx mics for these isolates were greater than 128 microg/ml but were drastically reduced in the presence of 4 microg of clavulanic acid per ml. these strains were also resistant to ceftriaxone, cefpodoxime, and aztreonam but were susceptible to ceftazi ...200415364979
microbiological quality of fermented cassava flour 'kpor umilin'.a study was conducted on the microbiological quality of traditionally fermented cassava flour 'kpor umilin' and a laboratory modification of the method developed with a view to upgrading the traditional process. microbial analysis of samples from both processing methods was evaluated using standard microbiological methods. the bacterial count in the traditionally processed flours ranged from 2.7 x 10(3) to 1.2 x 10(7) cfu/g, while the modified sample had a count of 3.5 x 10(2) cfu/g. the predomi ...200415369985
diagnosis of proteus syndrome was correct. 200415372511
mutation analysis of the tumor suppressor pten and the glypican 3 (gpc3) gene in patients diagnosed with proteus syndrome.proteus syndrome is a complex hamartomatous disorder characterized by asymmetrical gigantism, epidermal nevi, vascular malformations, hamartomas, lipomas, and hyperostosis. since the syndrome was first described, many hypotheses have been proposed to explain its occurrence. the most plausible is happle's somatic mosaic hypothesis, but no somatic mutations in candidate genes have been reported to be clearly involved in proteus syndrome. however, germ-line pten mutations have been reported in pati ...200415372512
reassessment of the proteus syndrome literature: application of diagnostic criteria to published cases.the medical care of patients affected by rare disorders depends heavily on experiences garnered from prior cases, including those patients evaluated by the treating physician and those published in the medical literature. the utility of published cases is wholly dependent upon accurate diagnosis of those patients. in our experience, the rate of misdiagnosis in proteus syndrome (ps) is high. diagnostic criteria have been published, but these criteria have not been applied consistently and were pu ...200415372514
[urinary stones in tunisian infants, based on a series of 64 cases].to define the epidemiological and clinical characteristics of urinary stones in infants, to study the role of stone chemical analysis in the aetiological assessment of urinary stones and to define the various treatment modalities adapted to this age-group.200415373180
mucosal vaccination of mice with recombinant proteus mirabilis structural fimbrial proteins.proteus mirabilis, a common cause of urinary tract infection (uti), expresses several types of fimbria including mannose-resistant/proteus-like fimbriae (mrp), uroepithelial cell adhesin (uca), renamed non-agglutinating fimbriae (naf) by some authors, and p. mirabilis fimbriae (pmf), which are potentially involved in adhesion to the uroepithelium. in this study, we immunised different groups of mice with recombinant structural subunits of these fimbriae (mrpa, ucaa and pmfa) using two mucosal ro ...200415374007
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2002). i. susceptibility distribution].the bacterial strains isolated from 491 patients diagnosed as having urinary tract infections (utis) in 13 institutions in japan were supplied between august 2002 and july 2003. the susceptibilities of them to many kinds of antimicrobial agents were investigated. of them, 578 strains were estimated as causative bacteria and used for the investigation. the number of them was 578 strains consisting of 177 gram-positive bacterial strains (30.6%) and 401 gram-negative bacterial strains (69.4%). agai ...200415376785
[xanthogranulomatous pyelonephritis: clinical, radiological and pathologic characteristics].xanthogranulomatous pyelonephritis is an atypical chronic infection which simulates a malignant renal neoplasia, definitive diagnosis of which is obtained by pathologic study after surgical removal. in this paper we analyze our experience and compare it to that reported on the literature.200415382434
[profile of antimicrobial resistance of agents causing urinary tract infections in children].the management of urinary tract infection in children faces the problem of the emergence of resistant strains to antibiotics. the aim of this study is to precise the frequency of the different germs and their susceptibility to antibiotics.200415382465
in vitro antimicrobial properties of aqueous garlic extract against multidrug-resistant bacteria and candida species from nigeria.the antimicrobial effects of aqueous garlic extract (age) against 133 multidrug-resistant gram-positive and gram-negative bacterial isolates, including staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, haemophilus influenzae, salmonella typhi, pseudomonas aeruginosa, escherichia coli, shigella spp., and proteus spp., and against 10 candida spp. were studied. antibacterial activity of age by well-diffusion and macrobroth dilution method was chara ...200415383227
myocardial necrosis in a captive binturong (arctictis binturong).an adult male binturong, arctictis binturong, which had been anorexic and lethargic for seven days became acutely dyspnoeic and died under anaesthesia. a postmortem examination revealed left ventricular hypertrophy with a thrombus occluding the left ventricular chamber. histological findings included moderate to severe multifocal, vasculocentric myocardial degeneration and necrosis with fibrosis replacing myocardiocytes. escherichia coli and proteus mirabilis were grown on cultures. the animal's ...200415160846
images in clinical medicine. unusual cystitis. 200415163778
[acid phosphatase of ameba: amoeba proteus]. 200415174335
[bact-alert automatized system for blood cultures: 5 vs 7 days of incubation. first argentine multicentre study].bact-alert automatized system for blood cultures: 5 vs 7 days of incubation. first argentine multicentre study. between january and december 2001, we analyzed 80,141 blood cultures by the bact-alert system (14,960 fan aerobics, 3,855 fan anaerobic, 11,114 standards aerobics, 11,367 standards anaerobic, 12,054 pediatrics and 26,791 fan pediatrics bottles) and 44.235 series from 27.615 patients at eight hospitals of buenos aires city, one of la plata city and three of the buenos aires province. a ...200415174746
synthesis, absolute stereochemistry and molecular design of the new antifungal and antibacterial antibiotic produced by streptomyces sp.201.the absolute stereochemistry of the new antifungal and antibacterial antibiotic produced by streptomyces sp.201 has been established by achieving the total synthesis of the product. a series of analogues have also been synthesized by changing the side chain and their bioactivity assessed against different microbial strains. among them, 1e (r = c8h17) was found to be the most potent with mic of 8 microg/ml against mycobacterium tuberculosis, 12 microg/ml against escherichia coli and 16 microg/ml ...200415177476
characterization and serological classification of a collection of proteus penneri clinical strains.bacteria of the genus proteus, which are a common cause of urinary tract infections, are divided into four species: p. mirabilis, p. vulgaris, p. penneri, and p. hauseri, and three unnamed genomospecies, proteus 4, 5, and 6 (single-strain species p. myxofaciens was isolated from the gypsy moth). establishing the serological classification of these species would aid in completing the classification scheme of the whole genus proteus and in applying serological methods in diagnostic procedures and ...200415179326
screening of selected indigenous plants of lebanon for antimicrobial activity.the objective of this study is to test in vitro the antimicrobial efficacy of 39 water and 39 methanol extracts derived from different parts of 27 indigenous wild plant species that have been commonly used in lebanese folk medicine. the antimicrobial efficacy was determined using the single disk diffusion method, with 10 and 20 microl load extract volume per disc. nine test microorganisms were used namely, escherichia coli, proteus sp., pseudomonas aeruginosa, shigella dysenteria, salmonella ent ...200415182897
prosthesis culture 6 months after implantation and the effect of haematogenous seeding in a rat model.the aim of this study was to investigate the role of haematogenous seeding in late prosthesis infection. in all, 45 male wistar albino rats were divided into three equal groups. a small piece of silicone prosthesis was implanted into the scrotum of all rats under sterile condition and antibiotic prophylaxis. in the first group, all silicones were removed after 6 months without any complication and were cultured for bacterial growth. in the second group, a disc, which was saturated with staphyloc ...200415184914
interactions between high pressure homogenization and antimicrobial activity of lysozyme and was the objective of this work to evaluate the effect of high pressure homogenization on the activity of antimicrobial enzymes such as lysozyme and lactoperoxidase against a selected group of gram positive and gram negative species inoculated in skim milk. lactobacillus helveticus, lactobacillus plantarum and listeria monocytogenes were the most pressure resistant species while bacillus subtilis, pseudomonas putida, salmonella typhimurium, staphylococcus aureus, proteus vulgaris and salmonell ...200415193800
evaluation of the tensile strengths of four monofilament absorbable suture materials after immersion in canine urine with or without evaluate the tensile strength, elongation, and degradation of 4 monofilament absorbable suture materials that undergo degradation by hydrolysis in specimens of canine urine with various physical characteristics.200415198227
artificial neural networks for prediction of antibacterial activity in series of imidazole derivatives.artificial neural networks (anns) have been applied for the quantitative structure-activity relationships (qsar) studies of antibacterial activity against escherichia coli, serratia marcescens, proteus vulgaris, klebsiella pneumoniae and pseudomonas aeruginosa of a large series of new imidazole derivatives. antibacterial activity against individual bacteria, expressed as logarithm of reciprocal of the minimal inhibitory concentrations, log 1/mic, has been related to a number of physicochemical a ...200415200380
metalloantibiotics: synthesis and antibacterial activity of cobalt(ii), copper(ii), nickel(ii) and zinc(ii) complexes of kefzol.kefzol (kzl), a beta-lactam antibiotic, possesses various donor sites for interaction with transition metal(ii) ions [co(ii), cu(ii), ni(ii) and zn(ii)] to form complexes of the type [m(kzl)2]cl2 and [m(kzl)cl], with molar ratio of metal: ligand (m:l) of 1:2 and 1:1 respectively. these complexes were prepared and characterized by physicochemical and spectroscopic methods. their ir and nmr spectra suggest that kefzol potentially acts as a bidentate, tridentate as well as monoanionic tetradentate ...200415202497
[total infarction of the penis caused by entrapment in a plastic bottle].penile strangulation injuries are rare emergency situations, but need a fast urological emergency management for decompression of the penis and for removing of the foreign bodies. strangulation objects can be metallic or non-metallic objects and are usually used for erotic or autoerotic purposes, for masturbation or increasing erection. we report a case of a 62-year old male with a total infected gangrene of the penis caused by a strangulation of a plastic bottle neck which continued 14 days. a ...200415205739
degradation of raw or film-incorporated beta-cyclodextrin by enzymes and colonic bacteria.beta-cyclodextrin (beta-cd) is a suitable excipient for peroral use, which improves the solubility of lipophilic drugs, as well as for colon-specific drug release when it is mixed with coating polymers. the first aim of this work was to examine the suitability of various enzymes as a simple in vitro model for the glycolytic activity in the human colon. alpha-amylase (source aspergillus oryzae) and taka diastase (source a. oryzae) showed remarkable degradation capacity of free beta-cd, whereas ot ...200415207542
ultrastructure of proteus mirabilis swarmer cell rafts and role of swarming in catheter-associated urinary tract infection.proteus mirabilis is a common cause of catheter-associated urinary tract infection (c-uti). it blocks indwelling urethral catheters through the formation of extensive crystalline biofilms. the obstruction of urine flow can induce episodes of pyelonephritis, septicemia, and shock. p. mirabilis exhibits a type of motility referred to as swarming, in which multicellular rafts of elongated, hyperflagellated swarmer cells form and move rapidly in concert over solid surfaces. it has been suggested tha ...200415213138
evolution of acquired resistance to third-generation cephalosporins in enterobacteriaceae in a tunisian hospital 1993-2001.between january 1993 and december 2001, the overall frequency of resistance to third-generation cephalosporins in isolates of enterobacteriaceae from charles nicolle hospital, tunis, rose from 2.4% to 7.4%. klebsiella pneumoniae was the most prevalent species (56%), followed by escherichia coli (15%) and proteus mirabilis (9%). a rate of 49% was observed among isolates from paediatric patients in 1999, caused mostly by outbreaks in the neonatal intensive care unit of k. pneumoniae and p. mirabil ...200415214883
evaluation of the uro-quick, a new rapid automated system, for the detection of well-characterized antibiotic-resistant bacteria.the uro-quick system has been employed to detect antibiotic resistance in genotypically and/or phenotypically well-characterized bacterial species including those that might not be easily identified by routine procedure. in order to achieve full agreement between the antibiotic susceptibility results obtained by the reference method (nccls) and the uro-quick system, the optimal experimental conditions (inoculum size, time of incubation and antibiotic concentration) for each strain to be used by ...200415216942
extended-spectrum beta-lactamase-producing enterobacteriaceae in an italian intensive care unit: clinical and therapeutical this study we evaluated the prevalence of enterobacteriaceae and the epidemiology of esbl+ microorganisms in an icu of our institution over a 5-year period and analyzed the clinical features and outcomes of the infections caused by these microorganisms. the most frequent esbl+ isolate was proteus mirabilis (69 isolates, 58%); a high rate of positive results in the double-disk synergy test (dds) was also recognized for klebsiella pneumoniae (52 isolates, 51%), whereas this phenomenon was obser ...200415216948
resistance rates to commonly used antimicrobials among pathogens of both bacteremic and non-bacteremic community-acquired urinary tract infection.this study examined the distribution of organisms and their antimicrobial resistance in patients admitted due to acute bacteremic and non-bacteremic community-acquired urinary tract infection (uti). during a period of 1 year and 1 month, a total of 201 patients and 253 bacterial isolates were studied. fever higher than 38.5 degrees c was significantly more common in the bacteremic group than the non-bacteremic group (68% vs 48%; p<0.05). escherichia coli, klebsiella pneumoniae, pseudomonas aerug ...200415221039
antibiotic resistance of urinary tract pathogens and rationale for empirical intravenous therapy.empirical antibiotic treatment in urinary tract infection (uti) in children must rely on surveillance data on the epidemiology and resistance patterns of common uropathogens. a retrospective analysis of bacteria isolated from children with uti irrespective of underlying disease or pre-treatment was performed at the university hospital of freiburg, germany, in 1997, and from 1999 to 2001. in the first study period, 261 positive urine samples and in the second period 684 positive samples were anal ...200415221429
sensitivity and spectrum of bacterial isolates in infectious otitis determine the spectrum and antibiotic sensitivity pattern of organisms from aural swabs taken from cases of infectious otitis externa.200415228846
central venous catheter-related blood stream infections: incidence, risk factors, outcome, and associated determine the incidence, risk factors, outcome, and pathogens of central venous catheter-related bloodstream infections (cvc-bsis).200415232809
changing epidemiology of neonatal septic study the changing epidemiological pattern of micro-organisms as an aetiology of septic arthritis, and to correlate the pattern with the outcome of neonatal septic arthritis, in terms of joint function and morphology.200415237115
the antimicrobial activity of extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents.the antimicrobial activity of the chloroform, diethyl ether, acetone, petroleum ether, and ethanol extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents against 9 bacteria and fungi has been investigated. the extracts and pure compounds alone were found active against the same bacteria and the same yeasts. bacillus cereus, bacillus subtilis, staphylococcus aureus, streptococcus faecalis, proteus vulgaris, listeria monocytogenes, aero ...200415241936
isolation of bacteriophages from the oral isolate bacteriophages lytic for oral pathogens from human saliva, dental plaque and mature biofilms constituted from saliva-derived bacteria.200415242464
structure of the o-polysaccharide of proteus mirabilis o19 and reclassification of certain proteus strains that were formerly classified in serogroup o19.bacteria of the genus proteus are a common cause of urinary tract infections. the o-polysaccharide chain of their lps (o-antigen) defines the serological specificity of these bacteria. based on the immunospecificity of the o-antigens, two species, p. mirabilis and p. vulgaris, were classified into 49 o-serogroups, and more o-serogroups for strains of these species and p. penneri have been subsequently proposed.200415247885
[urolithiasis in hemodialysis patients]. 200415250327
gene cloning, expression and characterization of novel phytase from obesumbacterium proteus.the gene phya encoding phytase was isolated from obesumbacterium proteus genomic library and sequenced. the cleavage site of the phya signal peptide was predicted and experimentally proved. the phya protein shows maximum identity of 53% and 47% to phosphoanhydride phosphorylase from yersinia pestis and phytase appa from escherichia coli, respectively. based on protein sequence similarity of phya and its homologs, the phytases form a novel subclass of the histidine acid phosphatase family. to cha ...200415251209
lemierre's syndrome associated with septic pulmonary embolism: a case 18-year-old female patient suffering from a painful right-sided neck mass was admitted to our emergency service. computed tomography of the neck revealed thrombosis of the right internal jugular vein. computed tomography of the chest indicated septic pulmonary emboli in both lungs. blood and oropharyngeal cultures showed proteus that was sensitive to cefepime and amicasin. chemotherapy was administered for 12 days, however, her complaints continued with fulminant progression. an urgent decomp ...200415253264
in vitro activity of ave1330a, an innovative broad-spectrum non-beta-lactam beta-lactamase inhibitor.production of beta-lactamases is the main mechanism of beta-lactam resistance in gram-negative bacteria. despite the current use of clavulanic acid, sulbactam and tazobactam, the prevalence of class a and class c enzymes is increasing worldwide, demanding new beta-lactamase inhibitors. here we report the antimicrobial properties of ave1330a, a representative of a novel class of bridged bicyclico[3.2.1]diazabicyclo-octanones in combination with ceftazidime. materials and methods: ic(50) and kinet ...200415254025
kirby-bauer disc approximation to detect inducible third-generation cephalosporin resistance in enterobacteriaceae.resistance to beta-lactam antibiotics in enteric gram-negative bacilli may be difficult to detect using standard methods of either kirby-bauer disc diffusion (kbdd) or broth dilution for minimal inhibitory concentration (mic). this difficulty is due to genetic differences in resistance determinants, differences in levels of gene expression, and variation in spectra of enzymatic activity against the substrate beta-lactams used for susceptibility testing. we have examined 95 clinical isolates repo ...200415256000
radiologic manifestations of proteus syndrome.proteus syndrome is a sporadic disorder named for its highly variable manifestations. the disease causes tissue overgrowth in a mosaic pattern and may affect tissues derived from any germinal layer. the disease process is not usually apparent at birth but develops rapidly in childhood. common manifestations include macrodactyly, vertebral abnormalities, asymmetric limb overgrowth and length discrepancy, hyperostosis, abnormal and asymmetric fat distribution, asymmetric muscle development, connec ...200415256628
diffuse xanthogranulomatous pyelonephritis and staghorn calculus: report of one case.xanthogranulomatous pyelonephritis and staghorn calculus are rare in children. in this report, we describe a chinese boy without history of urinary tract infection who developed insidious onset of left flank pain. urine culture showed proteus mirabilis infection. sonography and computed tomography of the abdomen showed typical picture of xanthogranulomatous pyelonephritis and staghorn calculus. photomicrography showed characteristic lipid-laden macrophage aggregates. after nephrectomy, he was sy ...200415264707
lesson of the week: perils of pessaries.vaginal pessaries are widely considered to be a safe alternative to surgery in older women. we report a case of near fatal septicaemia in a 75-year-old woman associated with a shelf pessary, the presence of which was identified during an exploratory laparotomy. this case highlights the importance of the gynaecological history and examination when assessing older women with septicaemia of unknown source.200415271640
the three-dimensional structure of catalase from enterococcus faecalis.enterococcus faecalis haem catalase was crystallized using lithium sulfate at neutral ph. the crystals belong to space group r3, with unit-cell parameters a = b = 236.9, c = 198.1 a. the three-dimensional structure was determined by molecular replacement using a subunit of the proteus mirabilis catalase structure. it was refined against 2.3 a synchrotron data to a free r factor of 21.8%. like other catalases, the e. faecalis catalase is a homotetramer with a fold and structure similar to those o ...200415272159
incidence of enteric bacteria and staphylococcus aureus in day care centers in akwa ibom state, nigeria.the incidence of enteric bacteria and staphylococcus aureus in four day care centers in akwa ibom state was studied using culture techniques. the percentage frequencies of the isolates from 124 samples were staphylococcus aureus (33.9), escherichia coli (19.0), klebsiella sp (14.4), citrobacter sp (12.5) and proteus mirabilis (7.4). the sources of contamination were floors, chairs, skin, bed linen, door handles, fans, children's tables, walls, windows, ceiling, headmistress's table and chairs, d ...200415272770
application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections.a rapid and accurate method for detection for common pathogenic bacteria in foodborne infections was established by using oligonucleotide array technology. nylon membrane was used as the array support. a mutation region of the 23s rrna gene was selected as the discrimination target from 14 species (genera) of bacteria causing foodborne infections and two unrelated bacterial species. a pair of universal primers was designed for pcr amplification of the 23s rrna gene. twenty-one species (genera)-s ...200415279944
structure of the o-polysaccharide of proteus serogroup o34 containing 2-acetamido-2-deoxy-alpha-d-galactosyl phosphate.on mild acid degradation of the lipopolysaccharide of proteus vulgaris o34, strain ccug 4669, the o-polysaccharide was cleaved at a glycosyl-phosphate linkage that is present in the main chain. the resultant phosphorylated oligosaccharides and an alkali-treated lipopolysaccharide were studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, and the following structure of the branched tetrasaccharide phosphate repeating unit of the o-polysaccharide was established: [carbo ...200415280059
frequency and diversity of class a extended-spectrum beta-lactamases in hospitals of the auvergne, france: a 2 year prospective evaluate the frequency and diversity of extended-spectrum beta-lactamases (esbls) produced by enterobacteriaceae and pseudomonas aeruginosa in one french region.200415282240
epidermal nevus syndromes: clinical findings in 35 patients.of the patients with epidermal nevi, 10-18% may have disorders of the eye, nervous, and musculoskeletal systems. a predisposition to malignant neoplasms in ectodermal and mesodermal structures may also be found. there are six different epidermal nevus syndromes described so far: proteus, congenital hemidysplasia with ichthyosiform nevus and limb defect syndrome, phakomatosis pigmentokeratotica, sebaceous nevus, becker nevus, and nevus comedonicus. thirty-five patients with epidermal nevus syndro ...200415283784
anesthesia for proteus syndrome.proteus syndrome is a complex disorder comprising malformations and overgrowth of multiple tissues. the disorder is highly variable affecting tissues in a mosaic pattern. a 2-year-old boy with proteus syndrome, with epidermal verrucal naevus, hyperplastic lesions of connective tissue, hyperostosis, overgrowth of tubular bones, bilateral inguinal hernia, and juvenile intestinal polyposis was scheduled for vertebral magnetic resonance imaging (mri) for further evaluation of malignancies. in additi ...200415283831
emerging resistance among bacterial pathogens in the intensive care unit--a european and north american surveillance study (2000-2002).globally icus are encountering emergence and spread of antibiotic-resistant pathogens and for some pathogens there are few therapeutic options available.200415283864
epidermal nevus syndromes.the term "epidermal nevus syndrome" (ens) has been used to describe the association of epidermal hamartomas and extra-cutaneous abnormalities. epidermal nevi follow the lines of blaschko. the majority of the extra-cutaneous manifestations involve the brain, eye, and skeletal systems. several subsets with characteristic features have been delineated including the nevus sebaceous syndrome, proteus syndrome, child syndrome, becker nevus syndrome, nevus comedonicus syndrome, and phakomatosis pigment ...200415295924
investigating the antimicrobial activity of natural honey and its effects on the pathogenic bacterial infections of surgical wounds and conjunctiva.antimicrobial activities of 10-100% (wt/vol) concentrations of new honey, stored honey, heated honey, ultraviolet-exposed honey, and heated stored honey were tested against common human pathogens, including escherichia coli, entrobacter cloacae, pseudomonas aeruginosa, shigella dysenteriae, klebsiella sp., haemophilus influenzae, proteus sp., staphylococcus aureus, streptococcus hemolyticus group b, and candida albicans. antimicrobial activity of honey was tested in acidic, neutral, or alkaline ...200415298770
endophthalmitis after penetrating keratoplasty: microbiologic spectrum and susceptibility of present the microbial spectrum and susceptibilities of isolates in endophthalmitis following penetrating keratoplasty.200414962427
histamine-related hygienic qualities and bacteria found in popular commercial scombroid fish fillets in determine the histamine-related hygienic qualities and bacteria of scombroid fish fillets sold in traditional retail markets, 61 samples were collected from northern and southern taiwan. it was found that the content of volatile base nitrogen in most samples was below 25 mg/100 g, which is the regulatory level in taiwan. the ratio of unacceptable samples/total samples for aerobic plate count and escherichia coli was 100% and 15% in northern samples and 100% and 20% in southern samples, respec ...200414968980
d- and l-aspartic acids: new non-sugar components of bacterial polysaccharides.for the first time in bacterial polysaccharides, residues of d- and l-aspartic acids were identified as n-acyl substituents of 4-amino-4,6-dideoxy-d-glucose in the o-antigens of enterobacteria of the genera providencia and proteus.200414972025
isolation and characterization of integron-containing bacteria without antibiotic selection.the emergence of antibiotic resistance among pathogenic and commensal bacteria has become a serious problem worldwide. the use and overuse of antibiotics in a number of settings are contributing to the development of antibiotic-resistant microorganisms. the class 1 and 2 integrase genes (inti1 and inti2, respectively) were identified in mixed bacterial cultures enriched from bovine feces by growth in buffered peptone water (bpw) followed by integrase-specific pcr. integrase-positive bacterial co ...200414982773
longitudinal trends in fluoroquinolone resistance among enterobacteriaceae isolates from inpatients and outpatients, 1989-2000: differences in the emergence and epidemiology of resistance across organisms.we conducted a 12-year study to identify and compare trends in annual prevalence of fluoroquinolone (fq) resistance among enterobacteriaceae isolates obtained from inpatients and outpatients in our health care system. a total of 46,070 clinical enterobacteriaceae isolates underwent susceptibility testing. although there were significant increases in inpatient fq resistance for all enterobacteriaceae, fq resistance trends differed significantly across enterobacteriaceae (p<.001). for isolates obt ...200414986249
different susceptibilities of staphylococcus and gram-negative rods to epigallocatechin gallate.we examined the antibacterial effects of epigallocatechin gallate (egcg, the main constituent of tea catechins) against various strains of staphylococcus and gram-negative rods. compared to the minimum inhibitory concentrations (mics) of egcg against s. aureus, s. epidermidis, s. hominis, and s. haemolyticus (50-100 micro g/ml), higher mics (>or=800 micro g/ml) were observed against gram-negative rods, including escherichia coli, klebsiella pneumoniae, salmonella typhi, proteus mirabilis, pseudo ...200414991521
four-year survey of urinary tract infections in calves in israel.the prevalence of urinary tract infections in calves aged seven days to three months in three dairy cattle herds ranged from 0.5 to 1.6 per cent, with an average of 1.1 per cent. the mortality rate reached 16.1 per cent. the morbidity rate of the female calves was 1.4 per cent and that of the male calves 0.8 per cent. the bacteria isolated from urine, and from vaginal and preputial swabs were escherichia coli (35 per cent), corynebacterium renale (14 per cent), plasma coagulase-negative staphylo ...200414994858
structure of the neutral o-polysaccharide and biological activities of the lipopolysaccharide of proteus mirabilis o20.mild acid degradation of the lipopolysaccharide (lps) of proteus mirabilis o20 resulted in depolymerisation of the o-polysaccharide to give a repeating-unit pentasaccharide. a polysaccharide was obtained by o-deacylation of the lps followed by nitrous acid deamination. the derived pentasaccharide and polysaccharide were studied by nmr spectroscopy, including 2d 1h,1h cosy, tocsy, roesy, 1h,13c hmqc and hmqc-toscy experiments, along with chemical methods, and the following structure of the repeat ...200415013399
composition and the in vitro antimicrobial activities of the essential oils of some thymus species.the genus thymus (lamiaceae) is represented by 38 species (64 taxa) in turkey, and 24 of which are endemic to turkey. aerial parts of thymus longicaulis subsp. chaubardii var. chaubardii, t. zygioides var. lycaonicus, t. longicaulis subsp. longicaulis var. subisophyllus and t. pulvinatus collected from three different localities in balikesir province were subjected to hydrodistillation to yield essential oils which were subsequently analysed by gc and gc/ms. the main constituents of the oils wer ...200415018057
current issues in the management of urinary tract infections: extended-release ciprofloxacin as a novel treatment option.symptomatic urinary tract infections (utis) are a major public health concern in the developed world, accounting for almost 8 million annual outpatient and emergency department visits in the us alone, while also representing one of the most common hospital-acquired infections. the vast majority of uncomplicated utis are caused by the gram-negative bacillus escherichia coli, with other pathogens including enterococci, staphylococcus saprophyticus, klebsiella spp. and proteus mirabilis. effective ...200415018591
the status of antimicrobial resistance in taiwan among gram-negative pathogens: the taiwan surveillance of antimicrobial resistance (tsar) program, a nationwide surveillance of antimicrobial resistance (taiwan surveillance of antimicrobial resistance, tsar), isolates were collected from 21 medical centers and regional hospitals throughout taiwan over a three-month period in 2000 (tsar ii). this report summarizes susceptibility data of 7 common gram-negative bacilli (escherichia coli, klebsiella pneumoniae, serratia marcescens, proteus mirabilis, enterobacter cloacae, pseudomonas aeruginosa, and acinetobacter baumannii) in the tsar ii col ...200415023432
ascending to a uti vaccine? 200415040255
the possible influence of micro-organisms and putrefaction in the production of ghb in post-mortem biological recent years, the post-mortem production of the drug of abuse gamma-hydroxybutyric acid (ghb) in biological fluids (e.g. blood and urine) has caused various interpretative problems for toxicologists. previously, other researchers have shown certain microbial species (pseudomonas spp. and clostridium aminobutyricum) possess the necessary enzymes to convert gaba to ghb. a preliminary investigation involving putrefied post-mortem blood indicated there was no observed relationship between "endoge ...200415040914
tyrosine phenol-lyase and tryptophan indole-lyase encapsulated in wet nanoporous silica gels: selective stabilization of tertiary conformations.the pyridoxal 5'-phosphate-dependent enzymes tyrosine phenol-lyase and tryptophan indole-lyase were encapsulated in wet nanoporous silica gels, a powerful method to selectively stabilize tertiary and quaternary protein conformations and to develop bioreactors and biosensors. a comparison of the enzyme reactivity in silica gels and in solution was carried out by determining equilibrium and kinetic parameters, exploiting the distinct spectral properties of catalytic intermediates and reaction prod ...200415044726
antibacterial and antipeptide antibodies in japanese and finnish patients with rheumatoid has been suggested that proteus infection may be involved in the pathogenesis of rheumatoid arthritis (ra). bacterial and peptide immune responses in patients with ra and other control subjects were investigated in two geographically different populations. serum samples from finnish patients with early ( n=72) and advanced ( n=27) ra and 30 finnish healthy controls, as well as from japanese ra patients from two different locations: tokyo ( n=30) and otsu ( n=30), 18 patients with systemic lup ...200415045628
assay method for antitumor l-methionine gamma-lyase: comprehensive kinetic analysis of the complex reaction with l-methionine.l-methionine gamma-lyase (ec is a pyridoxal 5'-phosphate-dependent multifunctional enzyme. measuring the initial velocity of alpha-ketobutyrate production by alpha,gamma-elimination of l-methionine catalyzed by l-methionine gamma-lyase is not very feasible, because the enzyme simultaneously catalyzes both gamma-replacement and alpha,gamma-elimination. to develop an accurate enzyme assay, the comprehensive enzyme kinetics needed to be elucidated by progress curve analysis on the basis o ...200415051540
serological characterization of the o-specific polysaccharide of providencia alcalifaciens o23.the genus providencia belongs to the enterobacteriaceae family and currently consists of five species: p. alcalifaciens, p. heimbachae, p. rettgerii, p. rustigianii and p. stuartii. the serological classification scheme of p. alcalifaciens, p. rustigianii and p. stuartii includes 63 o-serogroups and 30 h-serogroups. the o-antigenic specificity is defined by the structure of the o-antigen (o-specific polysaccharide--ops), a part of the lipopolysaccharide (lps, endotoxin), one of the major compone ...200415053232
comparative study of the in vitro activity of a new fluoroquinolone, abt-492.the in vitro activity of a new fluoroquinolone, abt-492, was determined.200415056651
possible occurrence of a crabtree effect in the production of lactic and butyric acids by a floc-forming bacterial consortium.lactic and butyric acid production by bacterial flocs in a continuous culture obeyed different physiological constraints. the butyric acid rate of production was constant and independent of the growth rate [0.012 +/- 0.001 gbut/(l.h)], whereas lactic fermentation occurred only beyond a critical growth rate (0.25 +/- 0.05 h(-1)) and was apparently associated with an abrupt drop in biomass. principles of modeling used to describe a crabtree effect in saccharomyces cerevisiae were found to apply to ...200415057470
island nail transfer in the treatment of macrodactyly of the great toe: a case report.macrodactyly involving the great toe is difficult to treat when both the functional and cosmetic problems are addressed simultaneously. partial resection of the distal phalanx is an easy way to reduce the length of the involved toe. however, there are cosmetic concerns with loss of the toenail. the authors report on a clinical case in which shortening and debulking of the toe was performed while preserving the hallux nail. the nail complex was transferred as an island flap to the dorsal aspect o ...200415057859
effects of pnpg on cell growth cycle, motility machinery and quorum sensing in serratia marcescens.p-nitrophenylglycerol (pnpg) effectively inhibits swarming of the enterobacterium proteus mirabilis. the underlying mechanism of inhibition is unclear. we have now found that both pnpg also inhibits motility and swarming in another enterobacterium, serratia marcescens. while the peak promoter activities of the flagellar master operon (flhdcsm), the flagellin structural gene (hagsm) and the nuclease gene (nucasm) in s. marcescens increased with increasing pnpg concentration, the expression of the ...200415060680
maxillary sinus disease of odontogenic origin.odontogenic sinusitis is a well-recognized condition and accounts for approximately 10% to 12% of cases of maxillary sinusitis. an odontogenic source should be considered in patients with symptoms of maxillary sinusitis who give a history positive for odontogenic infection or dentoalveolar surgery or who are resistant to standard sinusitis therapy. diagnosis usually requires a thorough dental and clinical evaluation with appropriate radiographs. common causes of odontogenic sinusitis include den ...200415064067
[a case report of douglas abscess due to diverticulitis of the sigmoid colon associated with liver abscess]. 200415065348
ultra-violet resonance raman spectroscopy for the rapid discrimination of urinary tract infection bacteria.the ability to identify pathogenic organisms rapidly provides significant benefits to clinicians; in particular, with respect to best prescription practices and tracking of recurrent infections. conventional bioassays require 3-5 days before identification of an organism can be made, thus compromising the effectiveness with which patients can be treated for bacterial infections. we analysed 20 clinical isolates of urinary tract infections (uti) by ultra-violet resonance raman (uvrr) spectroscopy ...200415033230
etiology and susceptibility of urinary tract isolates in kosova.urinary tract infections are amongst the most common pathogenic infections with an increasing resistance to antimicrobials. the objective of this study was to determine the etiology and antimicrobial susceptibility patterns of urinary tract infection pathogens isolated in kosovo. a retrospective study was carried from urine samples of both inpatients and outpatients that were received in our laboratory throughout 2001. during the study period, 16500 urine samples were analysed, of which 4260 (25 ...200415037322
urinary bactericidal activity, urinary excretion and plasma concentrations of gatifloxacin (400 mg) versus ciprofloxacin (500 mg) in healthy volunteers after a single oral an open randomised double-crossover study 12 volunteers (six men, six women) received a single oral dose of gatifloxacin (400 mg) or ciprofloxacin (500 mg) to assess urinary bactericidal activity (in eight intervals up to 120 h) and pharmacokinetic (pk) parameters (up to 36 h). plasma concentrations and urinary excretion were determined by hplc with fluorescence detection, and urinary bactericidal titers (ubt) by microdilution-method, using antibiotic-free urine of each volunteer. the mean ma ...200415037323
synthesis of some new 6-methylimidazo[2,1-b]thiazole-5-carbohydrazide derivatives and their antimicrobial this study, 14 new compounds having 6-methyl-n2-(alkylidene/cycloalkylidene)imidazo[2,1-b]thiazole-5-carbohydrazide (3a-g), 3-[[(6-methylimidazo[2,1-b]thiazole-5-yl)carbonyl]amino]-4-thiazolidinone (4a-d) and 4-[[(6-methylimidazo[2,1-b]thiazole-5-yl)carbonyl]amino]-1-thia-4-azaspiro[4.4]nonan/[4.5]decan-3-one (4e-g) structures were synthesized. the structures of the compounds were elucidated by uv, ir, 1h-nmr, 13c-nmr, 1h-13c-cosy, mass spectra and elemental analysis. all compounds synthesize ...200415038463
molecular details of urease inhibition by boric acid: insights into the catalytic mechanism.the structure of the complex of urease, a ni-containing metalloenzyme, with boric acid was determined at 2.10 a resolution. the complex shows the unprecedented binding mode of the competitive inhibitor to the dinuclear metal center, with the b(oh)3 molecule bridging the ni ions and leaving in place the bridging hydroxide. boric acid can be considered a substrate analogue of urea, and the structure supports the proposal that the ni-bridging hydroxide acts as the nucleophile in the enzymatic proce ...200415038715
acetabular dysplasia associated with intra-articular lipomatous lesions in proteus syndrome. a case report. 200415069153
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