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role of swarming in the formation of crystalline proteus mirabilis biofilms on urinary catheters.the care of many patients undergoing long-term bladder catheterization is frequently complicated by infection with proteus mirabilis. these organisms colonize the catheter, forming surface biofilm communities, and their urease activity generates alkaline conditions under which crystals of magnesium ammonium phosphate and calcium phosphate are formed and become trapped in the biofilm. as the biofilm develops it obstructs the flow of urine through the catheter, causing either incontinence due to l ...200516091430
effects of commercial diazinon and imidacloprid on microbial urease activity in soil and sod.diazinon [o,o-diethyl o-2-isopropyl-6-methyl(pyrimidine-4-yl) phosphorothioate] and imidacloprid [1-(1-[6-chloro-3-pyridinyl]methyl)-n-nitro-2-imidazolidinimine] are applied to lawns for insect control simultaneously with nitrogenous fertilizers such as urea, but their potential effect on urease activity and nitrogen availability in turfgrass management has not been evaluated. urease activity in enzyme assays, washed cell assays, and soil slurries was examined as a function of insecticide concen ...200516091610
risk of nosocomial bacteria transmission: evaluation of cleaning methods of probes used for routine ultrasonography.there exists a small but definite risk of nosocomial infection transmission attributable to ultrasonography probes and coupling gels.200516092321
frequency and treatment of urinary tract infection in children subjected to urine culture, in sana'a, yemen.this study was carried out to estimate the frequency of urinary tract infection among children subjected to urine culture presenting to sam hospital, sana'a city, yemen and to determine the susceptibility of the isolated bacteria to the antibiotics.200516092643
an electrified catheter to resist encrustation by proteus mirabilis biofilm.we investigated the effect of iontophoresis produced by passing an electric current through silver electrodes attached to catheters on catheter encrustation by crystalline proteus mirabilis biofilm.200516094079
efficacy of dilute betadine solution irrigation in the prevention of postoperative infection of spinal surgery.prospective, single-blinded, randomized study.200516094267
proteus syndrome: a rare cause of hemihypertrophy and macrodactyly on bone scanning.proteus syndrome is a rare, sporadic genetic disorder characterized by overgrowth of multiple different tissues in a mosaic pattern. it is associated with connective tissue nevi, epidermal nevi, disproportionate overgrowth of multiple tissues, vascular malformations, characteristic tumors, and specific facial anomalies. joseph merrick, popularly known as the elephant man, is now believed to have suffered from proteus syndrome. a case of proteus syndrome and associated findings on bone scintigrap ...200516100477
[the effect of selective bowel decontamination and mechanical bowel preparation on bacterial translocation due to intraabdominal hypertension].the objective of our study is to evaluate the preventive effects of selective digestive decontamination (sdd) and mechanical bowel preparation in rats with experimentally induced bacterial translocation.200516100664
the family of serratia type pore forming toxins.the serratia marcescens hemolysin represents the prototype of a growing family of pore forming toxins. the available bacterial genome sequences reveal serratia hemolysin homologues in additional species. however, only s. marcescens hemolysin has been studied in great molecular detail. this family of toxins has nothing in common with the pore forming toxins of e. coli type (rtx toxins), the staphylococcus aureus alpha-toxin or the thiol activated toxin of group a beta-hemolytic streptococci (stre ...200516101433
potency and spectrum of tigecycline tested against an international collection of bacterial pathogens associated with skin and soft tissue infections (2000-2004).the antimicrobial activity of tigecycline, a novel glycylcycline, was evaluated against 5289 bacterial isolates recovered from hospitalized patients with skin and soft tissue infections during 2000-2004. strains were submitted from >70 medical centers in north america, latin america, and europe, and were tested centrally using reference broth microdilution methods. the top 10 ranking pathogens (95% of total) recovered included staphylococcus aureus (55.2%), enterococcus spp. (9.6%), pseudomonas ...200516105564
biochemical characterization of the chondroitinase abc i active site.cabc i (chondroitinase abc i) from proteus vulgaris is a galag (galactosaminoglycan) depolymerizing lyase that cleaves its substrates at the glycosidic bond via beta-elimination. cabc i cleaves a particularly broad range of galag substrates, including cs (chondroitin sulphate), ds (dermatan sulphate) and hyaluronic acid. we recently cloned and recombinantly expressed cabc i in escherichia coli, and completed a preliminary biochemical characterization of the enzyme. in the present study, we have ...200516108757
[weil-felix test]. 200516111212
a study on inhibitory effects of siğla tree (liquidambar orientalis mill. var. orientalis) storax against several bacteria.in this study, siğla (liquidambar orientalis mill.) storax (styrax) was investigated for antibacterial activity against aeromonas hydrophila, bacillus amyloliquefaciens, b. brevis, b. cereus, b. megaterium, b. subtilis, corynebacterium xerosis, enterobacter aerogenes, enterococcus faecalis, escherichia coli, e. coli o157:h7, klebsiella pneumoniae, listeria monocytogenes, micrococcus luteus, mycobacterium smegmatis, proteus vulgaris, pseudomonas aeruginosa, p. fluorescens, staphylococcus aureus a ...200516114094
development and evaluation of 16s rdna microarray for detecting bacterial pathogens in cerebrospinal fluid.the rapid identification of bacteria in cerebrospinal fluid (csf) is very important for patient management and antimicrobial therapies. we developed a 16s dna microarray-based method that targets 16s rdna and can directly detect bacteria from csf without cultivation. universal primers and specific probes were designed from the 16s rdna sequence data retrieved directly from the genbank database. the specificity of the assay is obtained through a combination of microarray hybridization and enzymat ...200516118409
bacteria isolated from blood, stool and urine of typhoid patients in a developing country.blood, stool and urine samples were collected from 100 patients diagnosed as having typhoid in 5 hospitals in akwa ibom state and analyzed for the presence of salmonella species and other bacteria. of the 100 blood samples screened, 55 (55%) were positive with the widal test and 39 (39%) were positive on blood culture. thirteen (14.1%) out of 92 urine samples were positive for bacterial growth, while 22 (26.8%) of the stool cultures were positive out of the 82 samples screened. those within the ...200516124436
a strategy for the control of catheter blockage by crystalline proteus mirabilis biofilm using the antibacterial agent triclosan.catheter blockage by crystalline proteus mirabilis biofilm is a common complication in patients undergoing long-term indwelling bladder catheterisation. previously we have shown that inflating the retention balloons of all-silicone catheters with triclosan solutions prevents the encrustation process. the aim of the present work was to examine whether this strategy is effective in latex-based catheters.200516126323
synthesis and antimicrobial activity of some novel derivatives of benzofuran: part 1. synthesis and antimicrobial activity of (benzofuran-2-yl)(3-phenyl-3-methylcyclobutyl) ketoxime derivatives.the reaction of salicylaldehyde with 1-phenyl-1-methyl-3-(2-chloro-1-oxoethyl) cyclobutane (1) and potassium carbonate was used to prepare (benzofuran-2-yl)(3-methyl-3-phenylcyclobutyl) methanone (2) for the starting reagent purposes. (benzofuran-2-yl)(3-phenyl-3-methyl cyclobutyl) ketoxime (3) was synthesized from the reaction of the compound (2) with hidroxylamine. new derivatives of (benzofuran-2-yl)(3-phenyl-3-methyl cyclobutyl) ketoxime (3) such as, o-glycidylketoxime (4) and o-phenylacylke ...200516129517
the incorporation of glucosamine into enterobacterial core lipopolysaccharide: two enzymatic steps are required.the core lipopolysaccharide (lps) of klebsiella pneumoniae is characterized by the presence of disaccharide alphaglcn-(1,4)-alphagala attached by an alpha1,3 linkage to l-glycero-d-manno-heptopyranose ii (ld-heppii). previously it has been shown that the wabh enzyme catalyzes the incorporation of glcnac from udp-glcnac to outer core lps. the presence of glcnac instead of glcn and the lack of udp-glcn in bacteria indicate that an additional enzymatic step is required. in this work we identified a ...200516131489
[prevalence of multidrug-resistant proteus spp. strains in clinical specimens and their susceptibility to antibiotics].proteus sp. are opportunistic microorganisms which cause urinary tract and wounds infections, bacteriaemia and sepsis. the aim of this study was analysis of prevalence of multidrug-resistant proteus sp. strains in clinical specimens and evaluation of their susceptibility to selected antibiotics. the study was carried out of 1499 proteus sp. strains were isolated in 2000-2003 from patients of departments and dispensaries of the university hospital cm in bydgoszcz umk in torun. the strains were id ...200516134389
[comparison of frequency and susceptibility to antimicrobials of bacterial strains of the family enterobacteriaceae isolated from patients hospitalised in intensive care units].the aim of the study was estimation of frequency and susceptibility to antimicrobial agents of gram-negative rods isolated from clinical specimens obtained from patients requiring intensive care, with emphasis on profile of the unit. the analysis comprised strains of gram-negative rods isolated from patients of two intensive care units (icus) of a tertiary care hospital (1200 beds). identification of cultured isolates was done using automated vitek and api systems (biomerieux, france). susceptib ...200516134390
[health accuracy of travnik cheese and quality standards of sanitary accuracy].the aim of work was to determine a health accuracy of sheep milk and the final product travnik cheese, as well as the quality standards of sanitary accuracy. the study was performed on 212 milk samples selected by random choice. the study involved observing the sanitary conditions of sheep milk and cheese production, as well as isolation and identification of causal agents of infective diseases in these products. the results showed the following agents isolated from milk and travnik cheese: esch ...200516134761
antibacterial activity of zuccagnia punctata cav. ethanolic extracts.the present study was conducted to investigate antibacterial activity of zuccagnia punctata ethanolic extract against 47 strains of antibiotic-resistant gram-negative bacteria and to identify bioactive compounds. inhibition of bacterial growth was investigated using agar diffusion, agar macrodilution, broth microdilution and bioautographic methods. zuccagnia punctata extract was active against all assayed bacteria (escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, ...200516137849
rheumatoid arthritis and proteus. 200516138503
[antibiotic resistance: from the results of onerba's networks to good antibiotic use]. 200516144065
[bacterial flora and mycosis of the vagina in women with symptoms of vaginal inflammation].to estimate the microbiological profile of vaginal flora in 30 women with gynecologic problems and 20 pregnant women complaining about pathological symptoms {pruritus, burning, vaginal discharge}.200516145858
contribution of the two conserved tryptophan residues to the catalytic and structural properties of proteus mirabilis glutathione s-transferase b1-1.pmgstb1-1 (proteus mirabilis glutathione s-transferase b1-1) has two tryptophan residues at positions 97 and 164 in each monomer. structural data for this bacterial enzyme indicated that trp97 is positioned in the helix a4, whereas trp164 is located at the bottom of the helix a6 in the xenobiotic-binding site. to elucidate the role of the two tryptophan residues they were replaced by site-directed mutagenesis. trp97 and trp164 were mutated to either phenylalanine or alanine. a double mutant was ...200515320869
clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle.bacteria contaminate the uterus of most dairy cattle after parturition and endometritis causes infertility. an endometritis score can be ascribed based on the vaginal mucus character and odour but it is not clear if the clinical score reflects the number of uterine bacteria or the inflammatory response. the present study tested the hypothesis that clinical evaluation of endometritis reflects the number of bacteria present in the uterus, and the acute phase protein response. swabs (n = 328) were ...200515589277
does the valve regulated release of urine from the bladder decrease encrustation and blockage of indwelling catheters by crystalline proteus mirabilis biofilms?we tested whether valve regulated, intermittent flow of urine from catheterized bladders decreases catheter encrustation.200515592093
brain abscess in children.to find out the clinical presentation, radiological characteristics, various underlying predisposing conditions and causative organisms of brain abscess in children in our setup.200519810297
synthesis and study of antibacterial and antifungal activities of novel 2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl] acetylamino]thiazoles.several 2-[[(benzoxazole/benzimidazole-2-yl)sulfanyl]acetylamino]thiazoles derivatives were synthesized by reacting 4-substituted-2-(chloroacetylamino)thiazoles with benzoxazole/benzimidazole-2-thioles in acetone and in the presence of k2co3. the chemical structures of the compounds were elucidated by ir, 1h-nmr, and fab(+)-ms spectral data. their antimicrobial activities against micrococcus luteus (nrll b-4375), bacillus cereus (nrrl b-3711), proteus vulgaris (nrrl b-123), salmonella typhimuriu ...200415595406
vascular anomalies and the growth of limbs: a review.growth of the limb in a child can be impaired, with the coexistence of a vascular malformation. in these vascular bone syndromes, altered growth is manifest as overgrowth or hypotrophy. the vascular malformation is usually complex and gets progressively worse with time. the two types of vascular anomalies in limbs, fast-flow and slow-flow, can be associated with limb length discrepancies. the fast-flow vascular malformations together with arteriovenous fistulae are part of parkes weber syndrome, ...200415599224
chemical structure, surface properties and biological activities of the biosurfactant produced by pseudomonas aeruginosa lbi from soapstock.pseudomonas aeruginosa lbi isolated from petroleum-contaminated soil produced rhamnolipids (rl(lbi)) when cultivated on soapstock as the sole carbon source. hplc-ms analysis of the purified culture supernatant identified 6 rl homologues (%): r(2) c(10) c(10) 28.9; r(2) c(10) c(12:1) 23.0; r(1) c(10) c(10) 23.4; r(2) c(10) c(12) 11.3; r(2) c(10) c(12) 7.9; r(2) c(10) c(12) 5.5. to assess the potential antimicrobial activity of the new rhamnolipid product, rl(lbi), its physicochemical properties w ...200415028876
[bacterial colonization of chronic wounds. studies on outpatients in a university dermatology clinic with special consideration of orsa].in this retrospective investigation, we documented the bacterial colonization of 79 patients with chronic wounds, who had been treated between january 2002 and may 2003 in an outpatient wound healing clinic of a university dermatology program. we isolated 106 facultative pathogenic bacterial strains of which 56 were staphylococcus aureus, 19 pseudomonas aeruginosa, 11 escherichia coli, 4 proteus mirabilis, 4 enterobacter cloacae, 2 serratia marcescens, 2 streptococcus group g und 8 further speci ...200415029435
[molecular mechanisms of cephalosporin resistance in gram-negative bacteria of the enterobacteriaceae family].extended spectrum beta lactamase genes were detected by the pcr in 87.6% of 231 enterobacteriaceae strains isolated in medical institutions of moscow, st. petersburg, tomsk and nazran that showed a decrease in their susceptibility to 3rd generation cephalosporins. alone or in various combinations tem type beta lactamases were detected in 43.3% of the isolates, 46.8 and 51.2% of the isolates produced shv type and ctx type beta lactamases respectively. combinations of 2 and 3 different determinant ...200415344391
[use of therapeutic polycomponent vaccine "immunovac vp-4" for the treatment of chronic bacterial prostatitis].a complex of clinical and laboratory examinations of patients with bacterial chronic prostatitis was carried out. the patients were divided into two groups: 55 patients treated by the method of immunotherapy and 20 patients (controls) undergoing basic therapy. immunotherapy was carried out with the use of polycomponent vaccine vp-4 containing staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris and escherichia coli antigens. vaccinal therapy was indicated in cases with infection focus ...200415346948
transcription of proteus mirabilis flaab.proteus mirabilis, a gram-negative urinary tract pathogen, has two highly homologous, tandemly arranged flagellin-encoding genes, flaa and flab. flaa is transcribed from a sigma(28) promoter, while flab is a silent allele. previous studies have demonstrated the presence of a family of hybrid flagellin genes, referred to as flaab. these genes are composed of the 5' end of flaa and the 3' end of flab, and are produced through excision of the intervening dna between the two genes. although the exis ...200415347745
digestive pathology of sea turtles stranded in the canary islands between 1993 and 2001.digestive lesions were observed in 84 of 136 sea turtles (128 caretta caretta, four chelonia mydas and four dermochelys coriacea) stranded in the canary islands between january 1993 and december 2001. in the oral cavity ulcerative and necropurulent stomatitis were the most frequently observed lesions, and in the oesophagus ulcerative and fibrinous oesophagitis, and traumatic oesophageal perforation were most frequently observed; all these lesions were mainly associated with the ingestion of fish ...200415357377
poly(vinyl chloride)-coated sol-gels for studying the effects of nitric oxide release on bacterial adhesion.nitric oxide (no) releasing sol-gel materials coated with poly(vinyl chloride) (pvc) films exhibit increased stability at ambient and physiological temperatures. the polymer overcoat, however, reduces the no fluxes by 5-35% over the initial week of release. the variation in no fluxes between unmodified and pvc-coated sol-gels is negligible after 7 days. the pvc polymeric layer provides controlled surface chemistry for systematic studies of the effects of no release on bacterial adhesion. as an e ...200415360321
surgical infections: a microbiological study.surgical infections are mostly polymicrobial, involving both aerobes and anaerobes. one hundred seventeen cases comprised of abscesses (n=51), secondary peritonitis (n=25), necrotizing fascitis (n=22) and wounds with devitalized tissues (n=19) were studied. the number of microorganisms isolated per lesion was highest in secondary peritonitis (2.32). the aerobe/ anaerobe ratio was 0.81 in secondary peritonitis and 1.8 in necrotizing fascitis. most secondary peritonitis (80%), necrotizing fascitis ...200415361989
bacterial resistance to antimicrobials in urinary isolates.escherichia coli accounted for about 80% of organisms in uncomplicated urinary tract infections (utis), followed by staphylococcus spp. especially staphylococcus saprophyticus, and proteus mirabilis. against e. coli isolates from patients with uncomplicated uti, faropenem was the most effective. up to 1999, fluoroquinolone-resistant isolates were not observed in patients with uncomplicated uti, but in 2001 fluoroquinolone-resistant e. coli isolates emerged and accounted for about 8%. various typ ...200415364302
urinary tract infections in african infants.urinary tract infection (uti) causes significant illness in the first 2 years of life. the diagnosis in most developing countries is often overlooked due to the tedious nature of obtaining urine from these young ones who would not void voluntarily. misdiagnosis very often lead to avoidable ill health and long-term renal damage. there is a need to diagnose uti in febrile infants to alert clinicians. a prospective study of febrile infants aged up to 12 months on admission was undertaken in a 6-mon ...200415364303
the role of unusual pathogens in prostatitis syndrome.a total of 1442 patients with symptoms of chronic prostatitis were examined over a 4-year period at the outpatient department for urogenital infections, university hospital for infectious diseases "dr. fran mihaljevic", zagreb, croatia. an infectious aetiology was determined in 1070 (74.2%) patients. in 561 of 1070 (52.4%) patients the inflammatory finding (>10 wbc/hpf) was found in expressed prostatic secretions (eps) or voided bladder urine (vb(3)). normal, <10 wbcs/hpf was found in 362 of 536 ...200415364308
nosocomial transmission of ctx-m-2 beta-lactamase-producing acinetobacter baumannii in a neurosurgery ward.three strains of cefotaxime (ctx)-resistant acinetobacter baumannii, fm0209680, fm0300106, and fm0301433, were isolated from transtracheal aspirate cultures of three patients with probable nosocomial infections in a neurosurgery ward in japan. the ctx mics for these isolates were greater than 128 microg/ml but were drastically reduced in the presence of 4 microg of clavulanic acid per ml. these strains were also resistant to ceftriaxone, cefpodoxime, and aztreonam but were susceptible to ceftazi ...200415364979
microbiological quality of fermented cassava flour 'kpor umilin'.a study was conducted on the microbiological quality of traditionally fermented cassava flour 'kpor umilin' and a laboratory modification of the method developed with a view to upgrading the traditional process. microbial analysis of samples from both processing methods was evaluated using standard microbiological methods. the bacterial count in the traditionally processed flours ranged from 2.7 x 10(3) to 1.2 x 10(7) cfu/g, while the modified sample had a count of 3.5 x 10(2) cfu/g. the predomi ...200415369985
diagnosis of proteus syndrome was correct. 200415372511
mutation analysis of the tumor suppressor pten and the glypican 3 (gpc3) gene in patients diagnosed with proteus syndrome.proteus syndrome is a complex hamartomatous disorder characterized by asymmetrical gigantism, epidermal nevi, vascular malformations, hamartomas, lipomas, and hyperostosis. since the syndrome was first described, many hypotheses have been proposed to explain its occurrence. the most plausible is happle's somatic mosaic hypothesis, but no somatic mutations in candidate genes have been reported to be clearly involved in proteus syndrome. however, germ-line pten mutations have been reported in pati ...200415372512
reassessment of the proteus syndrome literature: application of diagnostic criteria to published cases.the medical care of patients affected by rare disorders depends heavily on experiences garnered from prior cases, including those patients evaluated by the treating physician and those published in the medical literature. the utility of published cases is wholly dependent upon accurate diagnosis of those patients. in our experience, the rate of misdiagnosis in proteus syndrome (ps) is high. diagnostic criteria have been published, but these criteria have not been applied consistently and were pu ...200415372514
[urinary stones in tunisian infants, based on a series of 64 cases].to define the epidemiological and clinical characteristics of urinary stones in infants, to study the role of stone chemical analysis in the aetiological assessment of urinary stones and to define the various treatment modalities adapted to this age-group.200415373180
mucosal vaccination of mice with recombinant proteus mirabilis structural fimbrial proteins.proteus mirabilis, a common cause of urinary tract infection (uti), expresses several types of fimbria including mannose-resistant/proteus-like fimbriae (mrp), uroepithelial cell adhesin (uca), renamed non-agglutinating fimbriae (naf) by some authors, and p. mirabilis fimbriae (pmf), which are potentially involved in adhesion to the uroepithelium. in this study, we immunised different groups of mice with recombinant structural subunits of these fimbriae (mrpa, ucaa and pmfa) using two mucosal ro ...200415374007
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2002). i. susceptibility distribution].the bacterial strains isolated from 491 patients diagnosed as having urinary tract infections (utis) in 13 institutions in japan were supplied between august 2002 and july 2003. the susceptibilities of them to many kinds of antimicrobial agents were investigated. of them, 578 strains were estimated as causative bacteria and used for the investigation. the number of them was 578 strains consisting of 177 gram-positive bacterial strains (30.6%) and 401 gram-negative bacterial strains (69.4%). agai ...200415376785
[xanthogranulomatous pyelonephritis: clinical, radiological and pathologic characteristics].xanthogranulomatous pyelonephritis is an atypical chronic infection which simulates a malignant renal neoplasia, definitive diagnosis of which is obtained by pathologic study after surgical removal. in this paper we analyze our experience and compare it to that reported on the literature.200415382434
[profile of antimicrobial resistance of agents causing urinary tract infections in children].the management of urinary tract infection in children faces the problem of the emergence of resistant strains to antibiotics. the aim of this study is to precise the frequency of the different germs and their susceptibility to antibiotics.200415382465
in vitro antimicrobial properties of aqueous garlic extract against multidrug-resistant bacteria and candida species from nigeria.the antimicrobial effects of aqueous garlic extract (age) against 133 multidrug-resistant gram-positive and gram-negative bacterial isolates, including staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, haemophilus influenzae, salmonella typhi, pseudomonas aeruginosa, escherichia coli, shigella spp., and proteus spp., and against 10 candida spp. were studied. antibacterial activity of age by well-diffusion and macrobroth dilution method was chara ...200415383227
otitis externa infection in jordan. clinical and microbiological features.aims of this study were to investigate the clinical findings and to identify causative microorganisms and their susceptibility to antibiotics in otitis externa infection.200415448765
prulifloxacin.prulifloxacin, the prodrug of ulifloxacin, is a broad-spectrum oral fluoroquinolone antibacterial agent. after absorption, prulifloxacin is metabolised by esterases to ulifloxacin. the drug has a long elimination half-life, allowing once-daily administration. ulifloxacin is generally more active in vitro than other fluoroquinolones against a variety of clinical isolates of gram-negative bacteria, including community and nosocomial isolates of escherichia coli, klebsiella spp., proteus, providenc ...200415456336
[bacterial colonization and home mechanical ventilation: prevalence and risk factors].to investigate the prevalence of bacterial contamination of ventilators and colonization of patients, the bacteria implicated, and predisposing factors in noninvasive home ventilation.200415458614
pcr method based on the ogdh gene for the detection of salmonella spp. from chicken meat samples.in a previous paper, the ogdh gene that encodes 2-oxoglutarate dehydrogenase was isolated from salmonella typhimurium. the catalytic n-terminal region in the enzyme was found to be very specific for the salmonella species. therefore, the aim of the present study was to detect s. typhimurium in food sources using primers designed for ogdh-1 and ogdh-2 which were based on the salmonella-specific region of the ogdh gene. a simple polymerase chain reaction (pcr) detection method was developed to det ...200415459651
[antimicrobial activity of new original 2-aryliden-6-furfuryliden cyclohexanones and hexahydroindazoles on their basis].antimicrobial activity of 25 new original 2-aryliden-6-furfuryliden cyclohexanones and hexahydroindazoles based on them was studied. the majority of the compounds had low or moderate activity against the test cultures of staphylococcus spp., pseudomonas aeruginosa, proteus spp. and escherichia coil. compound xix (2,3-diphenyl-7-(5-nitrofurfuryliden)-3,3a,4,5,6,7-hexahydroindazole) with high antistaphylococcal activity was selected among several 2-aryliden-6-furfuryliden cyclohexanones and hexahy ...200415460019
species-dependent premature degradation of absorbable suture materials caused by infection--impact on the choice of thread in vascular surgery.in case of infection after vascular reconstruction, preference is often given to absorbable suture material with the aim of preventing persistence of infection. we have investigated the functional deficit of absorbable sutures on incubation with various different bacteria.200415461069
[microbiocenosis of the bowels in patients with gastroduodenal ulcers].gastroduodenal ulcer is accompanied by the bowels dysbacteriosis irrespective of the helicobacter pylori infection. any variant of ulcer treatment aggravates the bowels dysbacteriosis intensity. eradication therapy destroys bifidobacteria mostly.200415462314
correlation of extended spectrum beta lactamases production with cephalosporin resistance in gram negative bacilli.beta lactamase production is an important mechanism of developing resistance to beta lactam group of antibiotics. cephlosporins with extended spectrum of activity and stability were introduced to overcome this resistance, but soon production of extended spectrum beta lactamases (esbls), which are inducible in nature was reported. in this study klebsiella aerogenes--166, escherichia coli--120, citrobacter spps--116, pseudomonas spps--50, proteus spps--32 and s. typhi--16, strains were subjected t ...200415471144
digestion of cellulose and xylan by symbiotic bacteria in the intestine of the indian flying fox (pteropus giganteus).bats (order chiroptera) are a widely distributed group of mammals. pteropus giganteus belongs to the suborder megachiroptera. this bat consumes fruits and leaves as their major food. cellulose and xylan are the major composition of leaves. as they consume leaves in their diet, their digestive tract must contain cellulolytic and xylanolytic bacteria which help in the digestion of cellulose and xylan. the cellulolytic and xylanolytic bacteria were isolated and screened on berg's agar containing ce ...200415471682
evaluation of the microscan esbl plus confirmation panel for detection of extended-spectrum beta-lactamases in clinical isolates of oxyimino-cephalosporin-resistant gram-negative bacteria.we aimed to assess the performance of the microscan esbl plus confirmation panel using a series of 87 oxyimino-cephalosporin-resistant gram-negative bacilli of various species.200415471997
[antibodies to yersinia enterocolitica and proteus mirabilis in blood sera of rheumatoid arthritis patients].data on specific antibodies to the bacterial antigens of yersinia enterocolitica (serovars 03, 09) and proteus mirabilis in rheumatoid arthritis (ra) patients are presented. according to the data of some researchers, these organisms play some role in the pathogenesis of ra. the highest level of igg antibodies to y. enterocolitica antigens of both serovars was noted. the activity of specific igg antibodies to serovar 03 was higher than that to serovar 09. in the sera of ra patients with p. mirabi ...200415481928
detection of staphylococcus aureus enterotoxin a and b genes with pcr-eia and a hand-held electrochemical sensor.two electrochemical assays for detecting staphylococcus aureus enterotoxin a and b genes were developed. the assays are based on pcr amplification with biotinylated primers, hybridization to a fluorescein-labeled probe, and detection with horseradish peroxidase-conjugated anti-fluorescein antibody using a hand-held electrochemical detector. the limit of detection (lod) for both assays was approximately 16 copies of the sea and seb genes. the assays were evaluated in blinded studies, each with 81 ...200415488376
[microbiological quality of minimally processed vegetable salads].the microbiological condition of minimally processed vegetable salads (7-days durability) purchased in the retail network of szczecin has been assessed. the study included 14 kinds of salads manufactured by two producers. the total count of mesophilic aerobic bacteria and their spores, aid-forming bacteria (lactobacilli), titre of coliforms, occurrence of pathogenic bacteria and counts of moulds and yeast were determined. no pathogenic bacteria (salmonella, staphylococcus aureus, bacillus cereus ...200415493346
micredox--development of a ferricyanide-mediated rapid biochemical oxygen demand method using an immobilised proteus vulgaris biocomponent.biochemical oxygen demand (bod) is an international regulatory environmental index for monitoring organic pollutants in wastewater and the current legislated standard test for bod monitoring requires 5 days to complete (bod5 test). we are developing a rapid microbial technique, micredox, for measuring bod by eliminating oxygen and, instead, quantifying an equivalent biochemical co-substrate demand, the co-substrate being a redox mediator. elevated concentrations of proteus vulgaris, either as fr ...200415494235
microorganisms and calcium oxalate stone disease.microorganisms may have a role in the pathogenesis and prevention of kidney stones. the subjects of this review include nanobacteria, oxalobacter formigenes, and lactic acid bacteria. not reviewed here is the well-described role of infections of the urinary tract with proteus species and other urease-producing organisms associated with struvite stone formation. nanobacteria have been proposed to be very small (0.08-0.5 nm), ubiquitous organisms that could play a role in stone formation. the theo ...200415499215
four variants of the citrobacter freundii ampc-type cephalosporinases, including novel enzymes cmy-14 and cmy-15, in a proteus mirabilis clone widespread in poland.twenty-nine proteus mirabilis isolates from 17 polish hospitals were analyzed. the isolates were resistant to a variety of antimicrobials, and their patterns of resistance to beta-lactams resembled those of the constitutive class c cephalosporinase (ampc) producers. indeed, beta-lactamases with a pi of approximately 9.0 were found in all of the isolates, and they were subsequently identified as four ampc-type cephalosporinases, cmy-4, -12, -14, and -15, of which the two last ones were novel enzy ...200415504832
synthesis and antimicrobial activity of some netropsin analogues.nine novel lexitropsins were synthesized by linking two netropsin-like moieties through three different dicarboxylic acids; 9,10-dihydro-2,7-phenanthrenedicarboxylic acid; [(3-[[(carboxymethyl)amino]carbonyl]benzoyl)amino]acetic acid and indole-2,5-dicarboxylic acid. the netropsin residues were modified by the use of n-isopentylpyrrole, 5-methylthiophene or 5-isopropylthiazole heterocyclic building blocks in place of the usual n-methylpyrrole. the compounds were tested against five gram-positive ...200415505717
traditional medicine in sakarya province (turkey) and antimicrobial activities of selected species.traditional medicine in north-west of turkey (sakarya province) were studied during a 2 months field study by interviewing local informants from several villages. plant species used to treat infections were tested for antimicrobial activity. information was collected for 46 plant species from 30 families and for 5 animal species. twenty four of the plant species were cultivated. most used families were asteraceae, cucurbitaceae, lamiaceae and rosaceae and the most used plants were artemisia absi ...200415507351
plasmid-mediated multiple antibiotic resistance in proteus mirabilis isolated from patients with urinary tract infection.worldwide surveillance of antimicrobial resistance among urinary tract pathogens is useful to determine important trends and geographical variation for common gram negative and positive species. we initiated this study to learn the drug resistance pattern and its location (genomic or plasmid-borne) among the strains of proteus mirabilis, one of the most common causative agents of urinary tract infection (uti).200415507850
detection of ticarcillin-clavulonic acid susceptibility with microdilution method in citrobacter, hafnia, proteus and some gram negative bacteria.the broth dilution method has been regarded as a good alternative test for detection of susceptibilities to antimicrobial agents. in this study, the antimicrobial activity of ticarcillin-clavulonic acid (tim) was investigated by the minimal inhibitory concentration (mic) method on strains of aeromonas, citrobacter, hafnia, morganella, proteus, pseudomonas and gram negative bacteria isolated from raw milk. the isolate collection included 91 gram negative strains. fifty-one (56.04%) isolates were ...200415508433
rapidly rising prevalence of nosocomial multidrug-resistant, gram-negative bacilli: a 9-year surveillance study.to examine and quantify the temporal trends of nosocomial multidrug-resistant, gram-negative bacilli.200415518026
new structures of the o-specific polysaccharides of proteus. 4. polysaccharides containing unusual acidic n-acyl derivatives of 4-amino-4,6-dideoxy-d-glucose.the structures of the o-polysaccharides of the lipopolysaccharides of proteus mirabilis o7 and o49 were determined by chemical methods, mass spectrometry, including ms/ms, and nmr spectroscopy, including experiments run in an h2o/d2o mixture to reveal correlations for nh protons. the o-polysaccharides were found to contain n-carboxyacetyl (malonyl) and n-(3-carboxypropanoyl) (succinyl) derivatives of 4-amino-4,6-dideoxyglucose (4-amino-4-deoxyquinovose, qui4n), respectively. the behavior of qui4 ...200415521818
[chronic carriers of pathogen conjunctival bacteria. possible risks in cataract surgery].to ascertain the frequency and characteristics of chronic carriers of conjunctival pathogen bacteria among patients undergoing cataract surgery in our hospital, to allow the design of studies of their postsurgical endophthalmitis risk.200415523569
the bacteriology of open fractures in ile-ife, nigeria.the objective of this study is to determine the pattern of bacterial isolates and antibiotic sensitivity profile in open fractures. fifty-nine patients with open fractures of the lower limb long bones were studied. the majority of the cases were gustilo and anderson types ii 21(35.6%), and iiia 16(27.1%).200415523862
antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens.the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ...200415527919
intestinal microflora in 45 crows in ueno zoo and the in vitro susceptibilities of 29 escherichia coli isolates to 14 antimicrobial agents.microorganisms from 45 jungle crows (corvus macrorhynchos) captured from july to december 2002 at ueno zoo, tokyo were identified as escherichia coli, proteus mirabilis, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter cloacae, enterobacter agglomerans, pseudomonas maltophila, staphylococcus spp., micrococcus spp., and streptococcus spp. e. coli showed the highest rate of isolation (21.6%). in an in vitro susceptibility test for 29 isolates of e. coli to 14 antimic ...200415528866
effect of ceftazidime, amikacin and ciprofloxacin on biofilm formation by some enterobacterial clinical isolates.the effect of sub-minimal inhibitory concentrations of antimicrobial agents on the biofilm formationto polystyrene by escherichia coli, proteus vulgaris, providencia stuartii, and morganella morganii was investigated by examining eight clinical strains. all the isolates tested were efficient biofilm-forming strains in the microtiter plate assay, with crystal violet staining (od595 nm) ranging from 0.13 +/- 0.01 for p. stuartii er21870 to 1.23 +/- 0.02 for p. vulgaris er50120. the biofilm formati ...200415528892
endophthalmitis after pars plana vitrectomy: incidence, causative organisms, and visual acuity outcomes.to investigate the incidence, causative organisms, and visual acuity outcomes associated with endophthalmitis after pars plana vitrectomy.200415531315
genetic and physiological interactions in the amoeba-bacteria symbiosis.amoebae of the xd strain of amoeba proteus that arose from the d strain by spontaneous infection of legionella-like x-bacteria are now dependent on their symbionts for survival. each xd amoeba contains about 42,000 symbionts within symbiosomes, and established xd amoebae die if their symbionts are removed. thus, harmful infective bacteria changed into necessary cell components. as a result of harboring x-bacteria. xd amoebae exhibit various physiological and genetic characteristics that are diff ...200415537083
bacterial isolates in the sick young infant in ile-ife, nigeria.this prospective study was carried out to determine the bacterial pathogens and their antibiotic sensitivity profile in the sick young infant. all consecutive young infants with features suggestive of infection seen over 5 months at a comprehensive health centre in ile-ife, were screened for septicaemia and local bacterial infections. of the 121 sick young infants screened for infection, 94 (77.7 per cent) had confirmed bacterial infection and 54 (57.4 per cent) of the 94 had confirmed septicaem ...200415537715
[infectious spondylodiscitis in a health area of galicia (spain) from 1983-2003].to study the clinical-epidemiological characteristics of infectious spondylodiscitis (is) in adults of our health area in the late 20 years.200415538902
nosocomial outbreak by proteus mirabilis producing extended-spectrum beta-lactamase veb-1 in a korean university hospital.to examine the molecular mechanisms involved in the beta-lactam resistance of multidrug-resistant proteus mirabilis isolates that showed an unusual synergy between imipenem and ceftazidime in a korean hospital.200415546971
craniofacial hyperostoses in proteus syndrome -- a case report.description of a patient with a rare syndrome leading to the partial overgrowth of craniofacial bones.200415555524
beta-lactam susceptibilities and prevalence of esbl-producing isolates among more than 5000 european enterobacteriaceae isolates.in vitro susceptibility to 15 beta-lactam antibiotics was evaluated using enterobacteriaceae isolated during the sentry antimicrobial surveillance program. piperacillin/tazobactam was the most active penicillin against escherichia coli, proteus mirabilis, klebsiella oxytoca and klebsiella pneumoniae (94.9%, 98.3%, 87.4% and 82.9% of isolates susceptible). of the cephalosporins, cefepime was most effective against escherichia coli, proteus mirabilis and enterobacter cloacae (99.2%, 96.3% and 95.2 ...200415555882
modulation of swarming and virulence by fatty acids through the rsba protein in proteus mirabilis.after sensing external signals, proteus mirabilis undergoes a multicellular behavior called swarming which is coordinately regulated with the expression of virulence factors. here we report that exogenously added fatty acids could act as signals to regulate swarming in p. mirabilis. specifically, while oleic acid enhanced swarming, some saturated fatty acids, such as lauric acid, myristic acid, palmitic acid, and stearic acid, inhibited swarming. we also found that expression of hemolysin, which ...200415557604
mannose-resistant proteus-like fimbriae are produced by most proteus mirabilis strains infecting the urinary tract, dictate the in vivo localization of bacteria, and contribute to biofilm formation.proteus mirabilis, an etiologic agent of complicated urinary tract infections, expresses mannose-resistant proteus-like (mr/p) fimbriae whose expression is phase variable. here we examine the role of these fimbriae in biofilm formation and colonization of the urinary tract. the majority of wild-type p. mirabilis cells in transurethrally infected mice produced mr/p fimbriae. mutants that were phase-locked for either constitutive expression (mr/p on) or the inability to express mr/p fimbriae (mr/p ...200415557655
use of translational fusion of the mrph fimbrial adhesin-binding domain with the cholera toxin a2 domain, coexpressed with the cholera toxin b subunit, as an intranasal vaccine to prevent experimental urinary tract infection by proteus mirabilis.this is a follow-up to our previous study using an intranasal vaccine composed of mrph, the tip adhesin of the mr/p fimbria, and cholera toxin to prevent urinary tract infection by proteus mirabilis (x. li, c. v. lockatell, d. e. johnson, m. c. lane, j. w. warren, and h. l. mobley, infect. immun. 72:66-75, 2004). here, we have expressed a cholera toxin-like chimera in which the mrph adhesin-binding domain (residues 23 to 157) replaces the cholera toxin a1 adp-ribosyltransferase domain. this chim ...200415557656
[delayed brain abscess as a complication of a csf shunt].infection is a common complication of ventriculoperitoneal shunts, but the formation of a brain abscess related to shunt system is very rare. we present the case of a patient who developed a brain abscess around a ventricular catheter left in place after removing the valvular system.200415558205
phylogenetic characterization of legionella-like endosymbiotic x-bacteria in amoeba proteus: a proposal for 'candidatus legionella jeonii' sp. nov.the x-bacteria which initiated organismic association with the d strain of amoeba proteus in 1966 as parasites have changed to obligate endosymbionts on which the host depends for survival. owing to the difficulty in cultivating the bacteria in vitro, the identity of x-bacteria has not been determined. as the life cycle of x-bacteria is similar to that of legionella spp. in soil amoebae, we applied the polymerase chain reaction method with specific primers aimed at legionella spp. for the detect ...200415560823
cockroaches as potential vectors of nosocomial infections.to determine the possible role of cockroaches in the epidemiology of nosocomial infections.200415566034
lanthanide spectroscopic studies of the dinuclear and mg(ii)-dependent pvuii restriction endonuclease.type ii restriction enzymes are homodimeric systems that bind four to eight base pair palindromic recognition sequences of dna and catalyze metal ion-dependent phosphodiester cleavage. while mg(ii) is required for cleavage in these enzymes, in some systems ca(ii) promotes avid substrate binding and sequence discrimination. these properties make them useful model systems for understanding the roles of alkaline earth metal ions in nucleic acid processing. we have previously shown that two ca(ii) i ...200415568821
medical image. flank football. 200415570703
"craniofacial and dental manifestations of a case of proteus syndrome".proteus syndrome, which is a reference to the ancient greek god proteus, the polymorphous, was called to the attention of pediatricians. recognition of this syndrome has been difficult because of the variability of the syndrome's manifestations and because of its rarity. we describe a rare case of proteus syndrome and we discuss its differential diagnosis. our case presents with hemi facial hypertrophy, scrotal tongue, enamel hyperplasia as well as differential in the size of the dentition on bo ...200415573665
s-adenosylmethionine and pneumocystis.pneumocystis is a parasitic fungus causing pneumonia in immunosuppressed mammals and s-adenosylmethionine a key intermediary metabolite for all cells. other than a species of rickettsia bacteria and an aberrant strain of the protozoan amoeba proteus, pneumocystis is the only cell known unable to synthesize adomet; it must extract this key compound from its host. this was discovered using a culture system and confirmed by observing depletion of adomet in the plasma of infected animals. depletion ...200415321660
a novel glutamate-dependent acid resistance among strains belonging to the proteeae tribe of enterobacteriaceae.morganella, providencia and proteus strains were capable of surviving ph 2.0 for 1 h if glutamate was present. these strains did not have glutamic acid decarboxylase activity and the gadab genes were not detected in any of these bacteria. when exposed to ph 2.0 acid shocks, the survival rate of these bacteria was significantly increased with glutamate concentrations as low as 0.3 mm in the acid media. escherichia coli cells incubated at ph 3.4 consumed four times more glutamate and produced at l ...200415321677
proteus mirabilis zapa metalloprotease degrades a broad spectrum of substrates, including antimicrobial peptides.the 54-kda extracellular metalloprotease zapa is an important virulence factor of uropathogenic proteus mirabilis. while zapa has the ability to degrade host immunoglobulins (igs), the dramatic attenuation of virulence in zapa mutants suggests that this enzyme may have a broader spectrum of activity. this hypothesis was tested by in vitro assays with purified zapa and an array of purified protein or peptide substrates. the data reveal that many proteins found in the urinary tract are substrates ...200415322010
incidence of extended-spectrum beta-lactamases in clinical isolates of the family enterobacteriaceae in a pediatric hospital.the incidence of extended-spectrum beta-lactamases (esbls) was analyzed in enterobacteriaceae population circulating in the upper silesian child and mother health center in katowice (usc&mhc). altogether 1164 clinical specimens, collected from children hospitalized in 8 different hospital units of usc&mhc were investigated. five hundred and eighty-five clinical isolates of the family enterobacteriaceae were identified in specimens collected from 403 patients. two hundred and twenty-nine enteroba ...200415330264
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