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evaluation of antimicrobial activity of the stem bark of cylicodiscus gabunensis (mimosaceae).ethyl acetate (ea) extract of the stem bark of cylicodiscus gabunensis (cg) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: escherichia coli, klebsiella pneumoniae, shigella dysenteriae, shigella flexneri, morganella morganii, proteus vulgaris, proteus mirabilis, salmonella typhi, citrobacter freundii, enterobacter cloacae, enterobacter agglomerans, staphylococcus aureus, streptococcus feacalis, pseudomonas aeruginosa ...200620162076
composition and antimicrobial activity of essential oil from the fruits of amomum cannicarpum.essential oil from the fruits of amomum cannicarpum (wight) bentham ex baker (zingiberaceae) was hydrodistilled and characterized by gas chromatography-mass spectrometry (gc-ms). major constituents of the oil were beta-pinene (14.00%), elemol (10.45%) and alpha-cadinol (8.50%). thirty-three (91.48%) out of forty-one constituents were identified by gc-ms and subsequent data analysis. antimicrobial activity of the oil against gram-positive and gram-negative bacteria and some fungi, was determined ...200619839139
production and characterisation of monoclonal antibodies specific for escherichia coli o157:h7.seven monoclonal antibodies (mabs) specific for escherichia coli o157:h7, one of the major causes of haemorrhagic colitis in humans, were produced by immunising balb/c mice with the strain e. coli o157:h7. these monoclonal antibodies do not cross-react with other bacteria such as salmonella enterica serovar typhimurium, e. coli o14, e. coli jm109, s. enterica serovar enteritidis, s. panama, s. saintpaul, s. derby, s. muenchen, s. bredeney, s. hadar, yersinia enterocolitica, proteus vulgaris, shi ...200620429059
[epidemiology and etiology of urinary tract infections in the community. antimicrobial susceptibility of the main pathogens and clinical significance of resistance].urinary tract infections (uti) are a frequent problem in primary care. they occur mainly in women without underlying diseases and with no functional or structural anomalies of the urinary tract; consequently most cases are considered uncomplicated uti. etiology is influenced by factors such as age, diabetes, spinal cord injury, urinary catheterization, and other factors. escherichia coli causes 80-85% of acute episodes of uncomplicated cystitis. staphylococcus saprophyticus, proteus mirabilis, s ...200516854352
sterilisation of lucilia cuprina wiedemann maggots used in therapy of intractable wounds.three new techniques of sterilising maggots of lucilia cuprina for the purpose of debriding intractable wounds were studied. these techniques were utilisation of ultra-violet c (uvc) and maggot sterilisation with disinfectants. the status of sterility was checked on nutrient agar and blood agar and confirmed with staining. the indicators for the effectiveness of the methods were sterility and survival rate of the eggs or larvae. egg sterilisation with uvc had the lowest hatching rate (16+/-0.00% ...200516883286
bacteria fauna from the house fly, musca domestica (l.).the house fly, musca domestica has long been considered a potential agent for disease transmission ever since its existence. the general truth of this assertion remains undisputed till the present day in spite of increasing awareness toward an improved sanitation and better hygiene. the habitual movement of house fly from filthy substrata such as human faeces, animal excreta, carcasses, garbage, etc. makes them ideal candidates for disease transmission such as cholera, shigellosis, salmonellosis ...200516883292
[colonization of cental venous catheter in the intensive care units at the institute of cardiovascular disease "dedinje"--one year study].the application of central venous catheters (cvc) is associated with increased risk of microbial colonization and infection. the aim of present study was to assess the frequency of pathogens colonizing cvc and to determine their susceptibility pattern to various antimicrobial agents.200516812991
assessment of antibacterial activity of some topical otological solutions.otological solutions have long been used in the treatment of the bacterial and fungal infections of the ear. we investigated antibacterial activity of some otic solutions against the most common bacteria isolated from discharging ears.200516340276
hsp60 expression during carcinogenesis: a molecular "proteus" of carcinogenesis? 200516333979
in-vitro antibacterial and cytotoxic activity of cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes of the antibiotic drug cephalothin (keflin).keflin (kefl) interacts with co(ii), cu(ii), ni(ii) and zn(ii) metal ions leading to complexes of the type m(kefl)2cl2 and m(kefl)cl2, which have been characterized by physicochemical and spectroscopic methods. magnetic moment, ir, electronic spectral and elemental analyses data suggest that keflin behaves tridentately forming octahedral or trigonal bipyramidal complexes with the metal ions mentioned above. the new compounds have been screened in-vitro for antibacterial and cytotoxic activity ag ...200516335054
proteus syndrome with giant myelolipoma in the pelvis.proteus syndrome is a very rare and complex disorder with malformations and overgrowth of multiple tissues. this disorder was designated proteus syndrome by wiedemann et al. to denote its variable clinical expression. our patient presented with macrodactyly, cerebriform appearance and a huge abdominal mass. a biopsy under laparotomy was performed, and histopathological examination revealed myelolipoma. tumor resection was performed. to our knowledge, no case of proteus syndrome presenting a myel ...200516351661
determination of the antimicrobial properties of oligo-2-hydroxy-1-naphthaldehyde.oligo-2-hydroxy-1-naphthaldehyde (ohna) was synthesized by oxidative polycondensation using h2o2 (35%, aqueous solution), air o2 and naocl (34%, aqueous solution) by kaya and senol and the products were characterized by spectral techniques. antimicrobial activities of the first and second fractions of ohna were tested against corynobacterium xerosis ccm 2824, proteus vulgaris atcc 6897, staphylococcus epidermidis nrrl b-4877, s. aureus atcc 6538, enterobacter aerogenes atcc 13048, salmonella thy ...200516355978
in vitro activities of tigecycline, ertapenem, isepamicin, and other antimicrobial agents against clinically isolated organisms in taiwan.this study evaluated the in vitro activities of tigecycline, ertapenem, isepamicin, and other comparators against 861 bacterial isolates recovered from patients treated in three major teaching hospitals in 2003. mics to antimicrobial agents were determined by the agar dilution method. high rates of oxacillin resistance (58%) in staphylococcus aureus (60 isolates), and vancomycin resistance (21%) and quinupristin-dalfopristin non-susceptibility (39%) in enterococcus faecium (34 isolates) were fou ...200516359192
ctx-m-1 extended-spectrum beta-lactamase-producing proteus mirabilis in greece.to examine the dissemination of ctx-m-type extended-spectrum beta-lactamases (esbls) in clinical isolates of proteus mirabilis, 91 nonrepetitive esbl-producing p. mirabilis were collected from infected patients in a tertiary greek hospital during september, 2001, to may, 2004. a bla (ctx-m) gene was amplified in one isolate (strain a328), but bla (ctx-m) was not detected in any of the remaining esbl producers. sequencing results showed that p. mirabilis a328 produced a ctx-m-1 enzyme while pcr m ...200516359194
neurocutaneous syndromes and epilepsy-issues in diagnosis and management.epilepsy may be seen as a feature of many of the neurocutaneous syndromes. the challenge lies within the diagnosis of the specific disorder and ultimately control of the epilepsy. tuberous sclerosis is the most common of the disorders with a frequency of 4.9/100,000. an autosomal-dominant condition, diagnostic features may be unclear under 2 years of age. population studies suggest a prevalence of epilepsy of 78%, the majority presenting under the age of 12 months, with a high association betwee ...200516359466
differential activity of a lectin from solieria filiformis against human pathogenic bacteria.a lectin isolated from the red alga solieria filiformis was evaluated for its effect on the growth of 8 gram-negative and 3 gram-positive bacteria cultivated in liquid medium (three independent experiments/bacterium). the lectin (500 microg/ml) stimulated the growth of the gram-positive species bacillus cereus and inhibited the growth of the gram-negative species serratia marcescens, salmonella typhi, klebsiella pneumoniae, enterobacter aerogenes, proteus sp, and pseudomonas aeruginosa at 1000 m ...200516302091
comparative antimicrobial potency of meropenem tested against gram-negative bacilli: report from the mystic surveillance program in the united states (2004).the meropenem yearly susceptibility test information collection (mystic) program is a longitudinal resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of bacterial pathogens to carbapenems and other broad-spectrum agents. in 2004 (year six), the antimicrobial activity of 12 broad-spectrum agents was assessed against 2,799 gram-negative bacterial isolates submitted from 15 united states (usa) medical centers using clinical and laboratory standa ...200516323433
case analysis of purple urine-bag syndrome at a long-term care service in a community hospital.purple urine-bag syndrome (pubs) is a rare phenomenon in which the contents of urine bags turn purple or blue following patient catheterization. the condition often causes care givers tremendous distress. we investigated the prevalence and possible causes of pubs for a group of elderly patients.200516323555
microbiology of dacryocystitis among adults population in southern australia.retrospective study on microbiology of dacryocystitis cases in adults was carried out at the royal victorian eye and ear hospital (rveeh). sixty-four consecutive cases seen between march 2000 and june 2003 were included. seventy-nine organisms were isolated from the sixty-six samples (1.2/specimen). aerobic or facultative bacteria were recovered in 76 (96.2), fungi from two specimens (2.5%). and anaerobic bacteria from only one specimen (1.3%). staphylococcus aureus (27), was most common followe ...200516295714
lunatusin, a trypsin-stable antimicrobial peptide from lima beans (phaseolus lunatus l.).an anti-fungal peptide designated as lunatusin, with a molecular mass around 7kda, was purified from the seeds of chinese lima bean (phaseolus lunatus l.). the peptide was isolated using a simple protocol consisting of affinity chromatography on affi-gel blue gel and gel filtration on superdex 75. lunatusin exerted an anti-fungal activity toward fungal species such as fusarium oxysporum, mycosphaerella arachidicola and botrytis cinerea, and an antibacterial action on, bacillus megaterium, bacill ...200516269344
relationships between antimicrobial use and antimicrobial resistance in gram-negative bacteria causing nosocomial infections from 1991-2003 at a university hospital in taiwan.this study was conducted to evaluate the relationship between antimicrobial resistance and antimicrobial use in a university hospital in taiwan. disk susceptibility data of escherichia coli, klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, proteus spp., pseudomonas aeruginosa, acinetobacter spp., stenotrophomonas maltophilia and other non-fermentative gram-negative bacilli causing nosocomial infections were evaluated. data on annual patient-days and annual consumption (defined d ...200516280243
structures of the o-polysaccharides and classification of proteus genomospecies 4, 5 and 6 into respective proteus serogroups.an acidic branched o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide (lps) of proteus genomospecies 4 and studied by sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, roesy and h-detected 1h, 13c hsqc experiments. the following structure of the pentasaccharide repeating unit of the o-polysaccharide was established, which is unique among proteus polysaccharide structures: [structure: see text] where qui3nac stands for 3 ...200516262693
bacterial flora of the low male genital tract in patients consulting for infertility.the physiological aerobic bacterial flora of the low male genital tract was determined. this prospective study was performed on 600 semen specimens collected from 543 asymptomatic males consulting for infertility. semen cultures were sterile in 28.8%, with a polymicrobial flora and/or absence or low titres of ureaplasma urealyticum in 49.3%, and with one or two aerobic and facultative bacteria > or =1 x 10(3) cfu ml(-1) and/or u. urealyticum with titres > or =10(4) ccu ml(-1) (colour changing un ...200516266393
[dercum's disease: a case report].a case of dercum's disease in a 51-year-old obese woman with a history of brain vasculitis, and painful subcutaneous multiple lipomas is described. this disease, included in the category of rare diseases by the world health organization, first described in 1892 by francis xavier dercum, is characterized by its prevalence among women, its familiarity, by the presence of multiple painful subcutaneous lipomas and its association with obesity, hypercholesterolemia and asthenia. the disease has to be ...200516250185
characterization and biological activities of two copper(ii) complexes with diethylenetriamine and 2,2'-bipyridine or 1,10-phenanthroline as ligands.two new mixed ligand copper(ii) complexes with diethylenetriamine, 2,2'-bipyridine and 1,10-phenanthroline have been synthesized. the crystal and molecular structures of [cu(dien)(phen)](clo(4))(2) and [cu(dien)(bipy)](bf(4))(2) (dien=diethylenetriamine, phen=1,10-phenanthroline, bipy=2,2'-bipyridine) were determined by x-ray crystallography from single crystal data. these two complexes have similar structures. the epr spectral data also suggest that these complexes have distorted square pyramid ...200516257723
outcome of bacterial culture from mastoid granulations: is it relevant in chronic ear disease?to detect the presence of bacteria in mastoid granulations and compare its prevalence in both types of chronic suppurative otitis media (csom). to find out if stage of disease activity, age, duration of disease, and aditus patency relate to obtaining positive cultures.200516259653
paraparesis caused by an angiolipomatous hamartoma in an adolescent with proteus syndrome and scoliosis.this 16-year-old boy with proteus syndrome suffered acute spinal cord compression secondary to hemorrhage into an extradural paraspinal angiolipomatous hamartoma. a magnetic resonance (mr) imaging study performed 12 years earlier had revealed the presence of this tumor at the apex of a mild thoracic scoliosis, which had been stable during the intervening decade. spinal cord neurological deficit in proteus syndrome is rare. it can arise either from vertebral abnormalities and kyphoscoliotic defor ...200516238085
[effect of the proteolytic enzymes of bacillus licheniformis and the lysoamidase of lysobacter sp. xl1 on proteus vulgaris and proteus mirabilis].preparations of culture liquid of three bacullus licheniformis strains (s, 103, and 60.4) and the enzymatic preparation lysoamidase from culture liquid of lysobacter sp. strain xl1 actively lysed preliminarily autoclaved cells of gram-negative bacteria proteus vulgaris and p. mirabilis. living proteus cells treated with these enzymatic preparations were lysed during their subsequent autoclaving. inoculation of enzyme-treated proteus cells, taken either separately or in combination with one anoth ...200516240656
antibiotic resistance in outpatient urinary isolates: final results from the north american urinary tract infection collaborative alliance (nautica).the goal of the north american urinary tract infection collaborative alliance (nautica) study was to determine antibiotic susceptibility to commonly used agents for urinary tract infections against outpatient urinary isolates obtained in various geographic regions in the usa and canada. forty-one medical centres (30 from the usa and 11 from canada) participated, with each centre submitting up to 50 consecutive outpatient midstream urine isolates. isolates were identified to species level by the ...200516243229
[necrotizing fasciitis of the perineum secondary to a surgical treatment of bartholin's gland abscess].necrotizing fasciitis of the perineum is a rare but of fast evolution, and potentially fatal infectious disease process. it is characterized by progressive inflammation and extensive necrosis of subcutaneous tissue involving the fascia and other adjacent tissues. this infection may be idiopathic or secondary to local trauma or pelvic surgery. its mortality rate is 20%. we report a case of necrotzing fasciitis of the perineum in a 34-year-old woman following incision and drainage of bartholin's g ...200516243570
resolving deep phylogenetic relationships in salamanders: analyses of mitochondrial and nuclear genomic data.phylogenetic relationships among salamander families illustrate analytical challenges inherent to inferring phylogenies in which terminal branches are temporally very long relative to internal branches. we present new mitochondrial dna sequences, approximately 2,100 base pairs from the genes encoding nd1, nd2, coi, and the intervening trna genes for 34 species representing all 10 salamander families, to examine these relationships. parsimony analysis of these mtdna sequences supports monophyly o ...200516243763
surveillance of extended-spectrum beta-lactamases from clinical samples and faecal carriers in barcelona, spain.the aim of the present study was to characterize and compare the extended-spectrum beta-lactamase (esbl)-producing organisms isolated from clinical samples and faecal carriers in 2001 and 2002.200516244084
antimicrobial activity of some medicinal plants from india.the results of a preliminary antimicrobial screening of the methanol extracts of zingiber officinale, asteracantha longifolia, citrus acida, salacia microsperma and tinospora cordifolia are reported.200516229969
bacterial flora of leg ulcers in patients admitted to department of dermatology, poznań university of medical sciences, during the 1998-2002 period.venous leg ulcers are an important cause of morbidity in a significant percentage of the world population. the percentage of leg ulceration, either active or healed, in the european population is about 1%-2%. the aim of this study was to analyze the rate of colonization and qualitative composition of the bacterial flora isolated from leg ulcers in patients admitted to dermatology department, poznań university of medical sciences, during the 1998-2002 period, with special reference to the infecti ...200516146619
analysis of antibiotic susceptibility and resistance of leg ulcer bacterial flora in patients hospitalized at dermatology department, poznań university hospital.effective treatment of frequently infected, poorly healing wounds such as leg ulcers due to chronic venous insufficiency poses a major clinical problem. antibiotic resistance in dermatology patients, especially those with non-healing leg ulcers, is a widespread phenomenon. various antibiotics, mainly broad-spectrum agents, are frequently and sometimes inappropriately prescribed, which often leads to the selection of antibiotic-resistant bacteria strains. the aim of this study was to analyze anti ...200516146620
in-vitro antibacterial, antifungal and cytotoxic activities of some coumarins and their metal complexes.a series of new antibacterial and antifungal coumarin-derived compounds and their transition metal complexes [cobalt (ii), copper (ii), nickel (ii) and zinc (ii)] have been synthesized, characterized and screened for their in vitro antibacterial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebacterium diphtheriae, staphylococcus aureus and streptococcus pyogenes bacterial strains ...200516206827
n,n-dichlorotaurine: chemical and bactericidal properties.the biogenous antimicrobial agent n-chlorotaurine (nct) converts by disproportionation to n,n-dichlorotaurine (ndct) at a rate proportional to acidity. this occurs at appreciable amounts already in weakly acidic biological systems. to understand the consequences of ndct formation, a thorough investigation of this undescribed compound was mandatory, which needed its synthesis. differently from nct, this was possible in the aqueous system using trichloroisocyanuric acid. while the free acid, cl(2) ...200516211659
increase of faecal bifidobacteria due to dietary oligosaccharides induces a reduction of clinically relevant pathogen germs in the faeces of formula-fed preterm infants.in a previous study on formula-fed preterm infants, we were able to demonstrate that dietary oligosaccharides (a mixture of 90% galacto-oligosaccharides and 10% fructo-oligosaccharides in a concentration of 1 g/dl) stimulate the growth of faecal bifidobacteria. in the present explorative analysis of this study, we focus on the effect of the dominance of bifidobacteria on the presence of clinically relevant pathogens (staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, ...200516214763
[prevalence and risk factors for bacteriuria in patients with cirrhosis].bacterial infections occur frequently in patients with cirrhosis and may worsen the disease outcome. we investigated the prevalence of bacteriuria in 500 consecutive patients with cirrhosis, in different child-pugh stages (41.4% a; 40.8% b; 17% c) and analysed the associated risk factors. most of the cirrhosis cases were virus related; alcohol abuse was recorded in 6.2% of the patients. bacteriuria was detected in 139 (27.8%) cases: 32.4% were more than 100,000 cfu/ml; 7.9% between 100,000 and 1 ...200516220030
plasma and urinary adrenomedullin levels in children with acute pyelonephritis.adrenomedullin (am), a novel peptide recently isolated from pheochromocytoma, eliciting vasorelaxing activity, is the strongest among all known peptides. am has been detected in the adrenal medulla, cardiac tissue, lung and kidney. immunohistochemical studies have demonstrated the localization of am in glomeruli, tubules and collecting cells of the kidney. clinically, plasma and urinary am levels are altered in patients with different renal disease. the present study aims to determine plasma and ...200516221100
extended spectrum beta-lactamases among gram-negative bacteria of nosocomial origin from an intensive care unit of a tertiary health facility in tanzania.resistance to third generation cephalosporins due to acquisition and expression of extended spectrum beta-lactamase (esbl) enzymes among gram-negative bacteria is on the increase. presence of esbl producing organisms has been reported to significantly affect the course and outcome of an infection. therefore infections due to esbl isolates continue to pose a challenge to infection management worldwide. the aim of this study was to determine the existence and to describe phenotypic and genotypic c ...200516225701
the ability of proteus mirabilis to sense surfaces and regulate virulence gene expression involves flil, a flagellar basal body protein.proteus mirabilis is a urinary tract pathogen that differentiates from a short swimmer cell to an elongated, highly flagellated swarmer cell. swarmer cell differentiation parallels an increased expression of several virulence factors, suggesting that both processes are controlled by the same signal. the molecular nature of this signal is not known but is hypothesized to involve the inhibition of flagellar rotation. in this study, data are presented supporting the idea that conditions inhibiting ...200516166542
phylogeny of the enterobacteriaceae based on genes encoding elongation factor tu and f-atpase beta-subunit.the phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor tu gene (tuf) and of their f-atpase beta-subunit gene (atpd). an 884 bp fragment for tuf and an 884 or 871 bp fragment for atpd were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. the atpd sequence analysis exhibited an indel specific to pantoea and tatumella species, showing, for the first time, a tight phylogenetic ...200516166704
genotyping of urinary and fecal proteus mirabilis isolates from individuals with long-term urinary catheters. 200516167137
influence of human ascitic fluid on the in vitro antibacterial activity of moxifloxacin.we investigated the in vitro influence of haf on the antibacterial activity of moxifloxacin against escherichia coli atcc 10798, escherichia coli k-12, proteus rettgeri (sanelli), staphylococcus aureus atcc 25923, staphylococcus aureus nctc 1808 and staphylococcus epidermidis atcc 12228. human ascitic fluid was obtained from 6 cirrhotic patients by paracentesis. the interaction effect was evaluated by the checkerboard technique. our results indicate the ability of human ascitic fluid to reduce m ...200516167519
bacteriology of surgical wound infections in a tertiary care hospital in turkey.to determine the spectrum of the pathogens cultured from surgical wound infections and assess their antimicrobial drug resistances.200516167704
in vitro and in vivo antibacterial activities of sm-216601, a new broad-spectrum parenteral carbapenem.sm-216601 is a novel parenteral 1beta-methylcarbapenem. in agar dilution susceptibility testing, the mic of sm-216601 for 90% of the methicillin-resistant staphylococcus aureus (mrsa) strains tested (mic(90)) was 2 microg/ml, which was comparable to those of vancomycin and linezolid. sm-216601 was also very potent against enterococcus faecium, including vancomycin-resistant strains (mic(90) = 8 microg/ml). sm-216601 exhibited potent activity against penicillin-resistant streptococcus pneumoniae, ...200516189097
granulomatous inflammation of salt glands in ducklings (anas platyrhynchos) associated with intralesional gram-negative bacteria.the "nasal glands" occur in many bird species and are powerful sodium ion-excretory organs. in ducks, they are located in supraorbital bony recesses. granulomatous inflammation of these glands occurs with an incidence of approximately 1% in ducklings (anas platyrhynchos), and is not associated with specific clinical symptoms. we investigated nine glands of eight animals with granulomas by gross pathology and histopathology, and compared results of bacteriology with 20 non-lesioned nasal glands. ...200516191707
identification and antimicrobial susceptibility of micro-organisms recovered from cutaneous lesions of human american tegumentary leishmaniasis in minas gerais, brazil.an evaluation of the microbiota present in cutaneous ulcers from 31 patients with a clinical and parasitological diagnosis of american tegumentary leishmaniasis (atl) was carried out by the standard filter paper disc technique, including antimicrobial susceptibility of the bacterial isolates. microbial examination indicated that 21 patients (67.7%) were contaminated with one to four bacteria and some of them also with yeast. a total of 142 micro-organisms were isolated. staphylococcus aureus was ...200516192439
multiple origins and incursions of the atlantic barnacle chthamalus proteus in the pacific.chthamalus proteus, a barnacle native to the caribbean and western atlantic, was introduced to the pacific within the last few decades. using direct sequencing of mitochondrial dna (coi), we characterized genetic variation in native and introduced populations and searched for genetic matches between regions to determine if there were multiple geographical sources and introduction points for this barnacle. in the native range, we found great genetic differences among populations (max. f(st) = 0.6 ...200516202091
predictive modelling of growth and measurement of enzymatic synthesis and activity by a cocktail of selected enterobacteriaceae and aeromonas hydrophila.the possibility was examined of developing a predictive model that would predict food spoilage by combining microbial growth (increase in cellular number) with extracellular enzymatic activity of a cocktail of five strains of enterobacteriaceae: escherichia coli, enterobacter agglomerans, klebsiella oxytoca, klebsiella pneumoniae and proteus vulgaris and one aeromonas hydrophila strain. estimations of growth and enzyme activity were made within a three-dimensional matrix of conditions: temperatu ...200516154655
hemihyperplasia with ehlers-danlos syndrome like skin changes.hemihyperplasia is a condition characterized by asymmetric overgrowth of one or more body parts. it may be an isolated finding or may be associated with various syndromes like beckwith-weidemann syndrome, klippel-trenaunay-weber syndrome, proteus syndrome etc. pigmentary skin lesions and capillary malformations are commonly seen in cases of hemihyperplasia. we report a case of hemihyperplasia with associated skin hyperextensibility, varicose veins, skin fragility, easy bruising and papery scars, ...200516155424
extended spectrum beta-lactamase-producing klebsiella pneumoniae chronic ambulatory peritoneal dialysis peritonitis treated successfully with polymyxin b.peritonitis is not an infrequent complication of inpatients with chronic ambulatory peritoneal dialysis (capd). capd peritonitis may be related to the catheter or secondary to perforation of an intra-abdominal viscus. the most common organisms usually associated with capd peritonitis are staphylococcus aureus and staphylococcus epidermidis (coagulase-negative staphylococci). rarely, aerobic gram-negative bacilli have been the causative agents of capd peritonitis. the treatment of capd peritoniti ...200516157192
purple urine bag syndrome in a hemodialysis patient.purple urine bag syndrome (pubs) is an uncommon disorder, in which the plastic disposable urinary catheter bag turns purple or blue following hours or days of urinary catheterization. the purple discoloration results from indirubin dissolved in the plastic mixing with indigo in the urine. bacteria possessing indoxyl sulfatase degrade indoxyl sulfate into indirubin and indigo. indoxyl sulfate is derived from the metabolism of tryptophan. pubs usually occurs in chronic catheterized elderly women w ...200516157987
[efficacy of probiotic bactisporin in therapy of intrahospital urinary infection].we studied efficacy of bactisporin (probiotic based on aerobic spore-forming culture bacillus subtilis, strain 3h) in combined treatment of intrahospital urinary infection (iui) in 36 patients with infravesical obstruction. these patients developed postoperative complications due to iui caused by antibiotic-resistant hospital flora. the control group consisted of 35 patients matched by age and disease given conventional postoperative etiotropic therapy. bacterial translocation enables bactispori ...200516158748
anti-bacterial activity of euphorbia fusiformis--a rare medicinal herb.euphorbia fusiformis buch.-ham. ex. d.don (euphorbiaceae) is a rare medicinal herb. aqueous and organic solvent extracts of the leaves and rootstocks were investigated for anti-bacterial properties by using disc diffusion and well-in agar methods, against pathogenic strains of gram positive (bacillus subtilis and staphylococcus aureus) and gram negative bacteria (escherichia coli, klebsiella pneumoniae, proteus vulgaris, pseudomonas aeruginosa, salmonella typhii a and salmonella typhii b). the d ...200516159702
[surveillance of susceptibility of clinical isolates to cefmetazole between 2000 and 2003].for the post-marketing surveillance of cefmetazole (cmz, cefmetazon), mics of injectable beta-lactam antibacterials including cmz against clinical isolates from 15 medical institutions all over japan are measured yearly and the incidence rates of resistance in various species are also evaluated. in the first surveillance from june 2000 to march 2001, 574 isolates of 13 species were tested, 548 isolates of the same 13 species were tested in the second surveillance from april 2001 to march 2002, a ...200516161756
regional variation in the prevalence of extended-spectrum beta-lactamase-producing clinical isolates in the asia-pacific region (sentry 1998-2002).we examined the prevalence of extended-spectrum beta-lactamase (esbl)-producing strains of klebsiella pneumoniae, klebsiella oxytoca, escherichia coli, proteus mirabilis, citrobacter koseri, and salmonella spp. that were isolated as part of the sentry asia-pacific surveillance program between 1998 and 2002. during the study period, a total of 6,388 strains were gathered from 17 medical centers in 7 countries and examined for esbl production and hyperproduction of k. oxytoca chromosomal k1 beta-l ...200516165001
dentomaxillofacial imaging in proteus syndrome.proteus syndrome is a rare condition that involves atypical growth of the bones, skin and head and a variety of other symptoms. only a few authors have reported on the craniofacial manifestations so far. the authors present a case of a 7-year-old girl with proteus syndrome in which the facial skeleton showed unilateral overgrowth. the analysis of the radiological evaluation revealed a bialveolar prognathism, a skeletal class iii, a dolicocephalic growth pattern and a left convex face scoliosis. ...200515961602
spread of novel expanded-spectrum beta-lactamases in enterobacteriaceae in a university hospital in the paris area, france.in 2002, 28 non-duplicate enterobacterial isolates producing extended-spectrum beta-lactamases (esbls) were collected from infected patients at the bicêtre hospital in paris, france. escherichia coli was the predominant esbl-positive enterobacterial species, comprising ten (36%) of the isolates. ctx-m enzymes (ctx-m-3, ctx-m-10, ctx-m-14 and ctx-m-15) were produced by 11 (39%) of the isolates (six e. coli, two enterobacter cloacae, one enterobacter aerogenes, one proteus mirabilis and one citrob ...200515966981
first description of dha-1 ampcbeta-lactamase in proteus mirabilis.this report describes the first occurrence of the dha-1 ampcbeta-lactamase gene in proteus mirabilis. the organism was isolated from the vaginal flora of a pregnant woman in a french hospital. the dha-1 beta-lactamase gene was identified on the basis of phenotypic characteristics, pcr amplification and sequencing. antagonism between cefoxitin and the other cephalosporins suggested inducible production of the dha-1 enzyme.200515966982
in-vitro antibacterial, antifungal and cytotoxic properties of sulfonamide--derived schiff's bases and their metal complexes.a series of new antibacterial and antifungal schiff's bases derived from sulfonamides, as well as their transition metal complexes incorporating cobalt (ii), copper (ii), nickel (ii) and zinc (ii) were synthesized, characterized and screened for their in-vitro antibacterial activity against six gram-negative (escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi and shigella dysentriae) and four gram-positive (bacillus cereus, corynebacterium diphth ...200515968823
[antibiotic resistance transformation in community-acquired urinary infections].to detect the prevalence of uropathogens in community-acquired urinary tract infection in our environment, and the degree of sensitivity to antibiotics used as empirical treatment.200515970133
noninvasive biophotonic imaging for monitoring of catheter-associated urinary tract infections and therapy in mice.urinary tract infections (utis) are among the most common bacterial infections acquired by humans, particularly in catheterized patients. a major problem with catheterization is the formation of bacterial biofilms on catheter material and the risk of developing persistent utis that are difficult to monitor and eradicate. to better understand the course of utis and allow more accurate studies of in vivo antibiotic efficacy, we developed a catheter-based biofilm infection model with mice, using bi ...200515972473
antimicrobial susceptibility pattern of blood isolates from a teaching hospital in north india.bloodstream infections are associated with significant patient morbidity and mortality worldwide. in this study, we examined antimicrobial susceptibility patterns by reviewing the data on 5,704 blood samples that were collected from patients with fever/sepsis admitted to government medical college and hospital, chandigarh, india, over a period of 1 year from august 2003 to july 2004. among the 567 qualifying samples, pseudomonas aeruginosa (19.75%), escherichia coli (15.17%), klebsiella pneumoni ...200515973011
bioactivity guided isolation of antimicrobial compounds from lythrum salicaria.lythrum salicaria extracts showed activity against the phytopathogenic fungus cladosporium cucumerinum and activity against the bacteria staphylococcus aureus, proteus mirabilis and microccocus luteus. bioautography on thin-layer chromatograms was used to isolate the two antifungal triterpenoids oleanolic and ursolic acid. the hexahydroxydiphenoyl ester vescalagin was isolated as active principle of the antibacterial activity. furthermore, the flavon-c-glucosides vitexin, isovitexin, orientin an ...200515975734
structure of the o-polysaccharide of proteus mirabilis oc (ccug 10702) from a new proposed proteus serogroup o75.a neutral o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of proteus mirabilis oc (ccug 10702) and studied by sugar and methylation analyses and (1)h and (13)c nmr spectroscopy. the following structure of the tetrasaccharide repeating unit of the polysaccharide was established: [structure: see text]. based on the unique structure of the o-polysaccharide and serological data, we propose classifying p. mirabilis oc (ccug 10702) into a new separate proteus serogroup ...200515979596
proteus mirabilis bloodstream infections: risk factors and treatment outcome related to the expression of extended-spectrum beta-lactamases.bloodstream infection (bsi) due to proteus mirabilis strains is a relatively uncommon clinical entity, and its significance has received little attention. this study was initiated to evaluate risk factors and treatment outcome of bsi episodes due to p. mirabilis producing extended-spectrum beta-lactamases (esbls). twenty-five bsi episodes caused by p. mirabilis occurred at our hospital (ospedale di circolo e fondazione macchi, varese, italy) over a 7.5-year period. phenotypic and molecular metho ...200515980325
peptides selected for binding to a virulent strain of haemophilus influenzae by phage display are bactericidal.nontypeable haemophilus influenzae (nthi) is an obligate parasite of the oropharynx of humans, in whom it commonly causes mucosal infections such as otitis media, sinusitis, and bronchitis. we used a subtractive phage display approach to affinity select for peptides binding to the cell surface of a novel invasive nthi strain r2866 (also called int1). over half of the selected phage peptides tested were bactericidal toward r2866 in a dose-dependent manner. five of the clones encoded the same pept ...200515980377
proteus: identifying signatures in protein termini.proteus (protein terminus) is a web-based tool for the identification of short linear signatures in protein termini. it is based on a position-based search method for revealing short signatures in termini of all proteins. the initial step in proteus development was to collect all signature groups (sigs) based on their relative positions at the termini. the initial set of sigs went through a sequential process of inspection and removal of sigs, which did not meet the attributed statistical thresh ...200515980469
clinical management and outcome of childhood lung abscess: a 16-year experience.in order to evaluate the clinical manifestations, management and outcome of childhood lung abscess, a retrospective chart review of 27 pediatric patients with international classification of diseases, ninth revision-clinical modification (icd-9 cm) code of 503.1 (lung abscess) from august 1987 to august 2003 was conducted. among the 27 patients (14 males and 13 females), 30% (8/27) were primary lung abscess and 70% (19/27) had underlying chronic diseases (secondary lung abscess). the predisposin ...200515986068
selective iga immune unresponsiveness to proteus mirabilis fumarate reductase a-chain in rheumatoid arthritis.to determine if selective immune unresponsiveness to microbial antigens is associated with predisposition to rheumatoid arthritis (ra).200515996053
aetiology of catheter-associated bacteriuria in lagos university teaching hospital.a prospective study to determine the causative agents of catheter-related bacteriuria was carried out on 99 patients with median age of 55 years, on admission in the oncology, paediatric surgery and obstetric and gynaecology wards of the lagos university teaching hospital. there was significant bacteriuria in seventy-one (72%) of the 99 urine samples. all catheter urine samples were contaminated by day 5 gram-negative bacilli were predominant (89.3%) of which pseudomonas aeruginosa (26.2%) was t ...200515997255
[long-term efficacy of trus-guided transperineal needle aspiration and irrigation on persistent hematospermia].to evaluate the long-term efficacy of transrectal ultrasonography (trus)-guided transperineal needle aspiration and irrigation for persistent hematospermia.200515999493
ampc disk test for detection of plasmid-mediated ampc beta-lactamases in enterobacteriaceae lacking chromosomal ampc beta-lactamases.although plasmid-mediated ampc beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. these enzymes are typically produced by isolates of escherichia coli, klebsiella spp., proteus mirabilis, and salmonella spp. and are associated with multiple antibiotic resistance that leaves few therapeutic options. plasmid-mediated ampc beta-lactamases have been associated with false in vitro susceptibility to cephalosporins. many ...200516000421
[xanthogranulomatous pyelonephritis: presentation of an unusual case].xanthogranulomatous pyelonephritis is a morphologically and clinically unique manifestation of chronic pyelonephritis with the formation of pus or granulomas. the most frequent predisposing factors for the development of xanthogranulomatous pyelonephritis are urinary obstruction (e. g., stones, tumors, congenital anomalies and functional impairment) and infection of the collecting system.200516001341
evaluation of cd4(+)/cd8(+) status and urinary tract infections associated with urinary schistosomiasis among some rural nigerians.data on urinary schistosomiasis in nigeria are mainly epidemological. the knowledge of co-infections of urinary schistosomiasis and other pathogens are important epidemiological tools for the control and health benefits of the rural dwellers. the granulomatous reactions in urinary schistosomiasis is cd4(+) dependent. the cd8(+) is cytotoxic to parasites and it is activated by cd4(+). these parameters therefore participate in the immune responses to urinary schistosomiasis.200516006219
analysis of pten gene mutations in korean patients with cowden syndrome and polyposis syndrome.pten (phosphatase and tensin homologue deleted in chromosome 10) is a candidate tumor suppressor gene. mutations of this gene are responsible for pten hamartoma tumor syndromes, including cowden syndrome, bannayan-riley-ruvalcaba syndrome, proteus syndrome, and proteus -like syndromes. recently, pten mutations were identified in several human neoplasms. we analyzed the dna of various organs and lesions in korean patients with cowden syndrome, their family members, and patients with familial aden ...200516007494
etiological and clinical patterns of childhood urolithiasis in iraq.this study was conducted to evaluate the etiological and clinical characteristics of urolithiasis in iraqi children. from 1999 to 2004, 204 children with renal calculi were evaluated. the age range of the patients was 4 months to 14 years, 61.3% of the patients were under 5 years. male to female ratio was 2.8:1. the mean age at onset of symptoms was 3.2 years, and stone disease was diagnosed at a mean of 3.5 years. hematuria (44.6%) and pain (28.4%) were the main clinical presentation. of the 20 ...200516010596
proteus syndrome: an update.proteus syndrome is a complex disorder consisting variably of disproportionate, asymmetric overgrowth of body parts; cerebriform connective tissue nevi; epidermal nevi; vascular malformations of the capillary, venous, and lymphatic types; and dysregulated adipose tissue. serious complications may ensue, such as pulmonary embolism, cystic lung disease, and various neoplasms. somatic mosaicism, lethal in the nonmosaic state, is the best working hypothesis. although proteus syndrome data are consis ...200516010681
[synthesis and antibacterial activity of 3-(5-substituted phenyl-[1,3,4] oxadiazole-2-yl-methylenethio)-5-pyridin-3-yl-[1,2,4] triazole-4-yl-amines].to study on synthesis and antibacterial activity evaluation of polyheterocycles.200516011262
swarmer cell differentiation in proteus mirabilis.under the appropriate environmental conditions, the gram-negative bacterium proteus mirabilis undergoes a remarkable differentiation to form a distinct cell type called a swarmer cell. the swarmer cell is characterized by a 20- to 40-fold increase in both cell length and the number of flagella per cell. environmental conditions required for swarmer cell differentiation include: surface contact, inhibition of flagellar rotation, a sufficient cell density and cell-to-cell signalling. the different ...200516011745
molecular characterization of multidrug-resistant proteus mirabilis isolates from retail meat products.sixty-four multidrug-resistant isolates of proteus mirabilis were collected from retail meat products in oklahoma. the isolates showed four different patterns of antibiotic resistance based on their resistant phenotype and genotypes. most of these isolates were resistant to ampicillin, tetracycline, gentamycin, and kanamycin. class 1 integrons were detected as a common carrier of the antibiotic-resistant genes, such as aada1, aadb, and aada2. a few isolates (9%) contained class 2 integrons with ...200516013378
colouterine fistula mimicking pyometrium--diagnosis established with multi-detector computed tomography. 200516022134
pyrrolobenzodiazepine dimers: novel sequence-selective, dna-interactive, cross-linking agents with activity against gram-positive bacteria.pyrrolo[2,1-c][1,4]benzodiazepine (pbd) dimers are synthetic sequence-selective interstrand dna minor-groove cross-linking agents developed from anthramycins. we investigated the antibacterial activity of three dimers, sjg-136, drg-16 and elb-21, which differ in the structure of the pbd monomeric unit and the length of the linker region between the two identical pbd monomers.200516024592
[species-specific detection of proteus vulgaris and proteus mirabilis by the polymerase chain reaction].sets of primers for the species-specific detection of p. mirabilis and p. vulgaris by the polymerase chain reaction (pcr) were developed. as targets for these primers beta-lactamase and 16s rrna gene fragments were chosen on the basis of the multiple leveling of the sequences of the dna of all known p. mirabilis and p. vulgaris isolates. for differential detection oligonucleotides were selected in such a way that primers, specific for p. vulgaris, contained the non-paired nucleotide for p. mirab ...200516028510
preclinical evaluation of rapeseed, raspberry, and pine bark phenolics for health related effects.rapeseed, raspberry, and pine bark are promising bioactive sources of plant phenolics selected from among ca. 100 previously screened plant materials for in vitro preclinical evaluation of health related effects. phenolic extracts and isolated fractions of the selected materials were investigated for antioxidant, antimicrobial, antiinflammatory, and antimutagenic properties as well as for cell permeability. it was shown that rapeseed and pine bark phenolics and raspberry anthocyanins were good o ...200516028975
extended-spectrum beta-lactamases.the first beta-lactamase was identified in an isolate of escherichia coli in 1940. to date, there are >130 tem-type and >50 sulfhydryl variable (shv)-type beta-lactamases, mainly in e. coli, klebsiella pneumoniae, and proteus mirabilis but also in other members of the enterobacteriaceae family and in some nonenteric organisms, such as acinetobacter species. the incidence of expanded-spectrum beta-lactamases (esbls) varies, depending on which area of the globe the isolates originate from. esbls r ...200516032564
recalcitrant scoliosis in proteus syndrome.proteus syndrome is a rare congenital condition with various clinical features such as hemihypertrophy, macrodactyly, subcutaneous masses, brain-like hyperplasia of the soles and/or palms, epidermal naevi and scoliosis with other mesodermal malformations. multifocal overgrowth can affect various tissues causing severe functional and cosmetic disability, but intellectual and language development are mostly normal. orthopaedic problems include macrodactyly, hindfoot deformity, limb length inequali ...200516035717
antimicrobial susceptibility patterns of contemporary pathogens from uncomplicated urinary tract infections isolated in a multicenter italian survey: possible impact on guidelines.during 2004 four italian laboratories assessed the prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in female out-patients. a total of 600 urine samples from individuals aged 18-65 were studied. the overall prevalence of escherichia coli was 85.3%. klebsiella pneumoniae, staphylococcus saprophyticus, proteus mirabilis, enterococcus faecalis and other rarer species were far less represented. determination of the antibiotic susceptibility pattern of th ...200516038517
antibacterial and antioxidant activities of ethanol extract from paullinia cupana mart.the antibacterial and antioxidant activity of the ethanol extract from paullinia cupana var. sorbilis mart. (sapindaceae) seeds, commonly called guarana, was assessed towards selected bacteria as well as in different antioxidant models. the extract, at a concentration between 16 and 128 microg/ml, showed a significant antibacterial effect expressed as minimum inhibitory concentration (mic) against both gram-negative and gram-positive bacteria. in particular, pseudomonas aeruginosa (mic=16 microg ...200516040216
tigecycline.new antimicrobial agents are urgently needed for clinical use due to the increasing prevalence and spread of multidrug-resistant bacteria that are commonly responsible for serious and life-threatening diseases. the need to develop new agents that effectively overcome existing mechanisms of resistance displayed by bacteria resistant to currently available drugs has become paramount. tigecycline, the first in a new class of antimicrobials, the glycylcyclines, is an analogue of minocycline with add ...200516040625
application of electrospray ionization with fourier transform ion cyclotron resonance mass spectrometry for structural screening of core oligosaccharides from lipopolysaccharides of the bacteria proteus.electrospray ionization with fourier transform ion cyclotron resonance mass spectrometry (esi-fticrms) was used for screening and structural elucidation of core oligosaccharides isolated from lipopolysaccharides of bacteria of the genus proteus. mass spectra allowed the determination of the molecular masses with high accuracy and the estimation of the chemical heterogeneity of the samples. they did not, however, provide sufficient information to identify structural details of the branched oligos ...200516041825
fatal herpes simplex virus infection in darier disease under corticotherapy.a 67-year-old man is presented with longstanding and severe darier disease treated by topical antiseptics and potent corticosteroids, in combination with oral glucocorticoids and etretinate. after cardiac bypass surgery in 1997, the patient experienced herpes simplex virus (hsv type-1) infection of the skin that was treated by intravenous aciclovir. in 2003, he presented a widespread atypical exacerbation of his darier disease, involving the face, trunk, buttocks, intertriginous areas and arms. ...200516048763
in vitro and in vivo antibacterial activities of cs-023 (ro4908463), a novel parenteral carbapenem.cs-023 (ro4908463, formerly r-115685) is a novel 1beta-methylcarbapenem with 5-substituted pyrrolidin-3-ylthio groups, including an amidine moiety at the c-2 position. its antibacterial activity was tested against 1,214 clinical isolates of 32 species and was compared with those of imipenem, meropenem, ceftazidime, ceftriaxone, ampicillin, amikacin, and levofloxacin. cs-023 exhibited a broad spectrum of activity against gram-positive and -negative aerobes and anaerobes, including methicillin-res ...200516048932
effects of lipopolysaccharides from gram-negative bacteria on the level of thiols in blood platelets.lipopolysaccharide (endotoxin, lps) activates blood platelets and stimulates generation of free radicals in these cells. the mechanism of platelet activation induced by lps is not known. the aim of the present study was to examine how glutathione (gsh) and other thiol-containing compounds are involved in the oxidative stress in blood platelets caused by lps. the hplc technique has been used on the analysis of non-protein thiols from human blood platelets treated with lipopolysaccharides of diffe ...200516049662
gudair (ojd) vaccine self-inoculation: a case for early debridement. 200516053420
surveillance of antimicrobial resistance in bulgaria--a synopsis from bulstar 2003.we introduce bulgarian surveillance tracking antimicrobial resistance (bulstar) and make the first report on surveillance data for 2003. this longitudinal surveillance programme monitors the isolation and antimicrobial susceptibility of all clinically significant microorganisms isolated from blood cultures, cerebrospinal fluid, upper and lower respiratory tract, urine and wound samples in the participating microbiology laboratories. twenty eight public, 45 hospital and 6 private laboratories fro ...200516077213
[design and improvement of an elective culture medium for bacteriological diagnosis of salmonella infection].experimental studies have provided a nutrient broth for elective accumulation of salmonella (ma broth) and a differential diagnostic culture medium for selective isolation of the causative agents of salmonella toxic infections. the ma broth is used for bacteriological isolation of salmonellae from the low-infected material and for a sanitary-and-bacteriological study of meat-and- sausage and other foods for this pathogen. a combination of high accumulating and inhibiting properties of the design ...200516078535
inhibitor-based methods for detection of plasmid-mediated ampc beta-lactamases in klebsiella spp., escherichia coli, and proteus mirabilis.non-beta-lactam inhibitor-based methods were evaluated for detecting plasmid-mediated ampc beta-lactamases in klebsiella spp., escherichia coli, and proteus mirabilis. using clsi methodology and disks containing cefotetan alone and in combination with 400 mug of boronic acid, 9 of 10 positive control strains and 54 of 55 ampc-pcr-positive clinical isolates were detected. importantly 71% and 40% of these clinical isolates were susceptible by routine testing to ceftriaxone and ceftazidime, respect ...200516081966
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