Publications

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cysteine based novel noncompetitive inhibitors of urease(s)--distinctive inhibition susceptibility of microbial and plant ureases.based on the catalysis mechanism of urease, a homologous series of 10 cysteine derivatives (cysds) was designed and synthesized, and their inhibitory activities were evaluated for microbial ureases (bacillus pasteurii, bpu, and proteus mirabilis, pmu) and for a plant urease [jack bean (cavavalia ensiformis), jbu]. as already described, thiol-compounds might inhibit urease activity by chelating the nickel atoms involved in the catalysis process. in contrast to cysteine, which has been reported to ...200616859909
structures and serology of the o-antigens of proteus strains classified into serogroup o17 and former serogroup o35.bacteria of the genus proteus are facultative pathogens which commonly cause urinary tract infections. based on the serological specificity of the o-chain polysaccharide of the lipopolysaccharide (o-polysaccharide, o-antigen), strains of p. mirabilis and p. vulgaris have been classified into 60 serogroups. studies on the chemical structure and serological specificity of the o-antigens aim at the elucidation of the molecular basis and improvement of the serological classification of these bacteri ...200616868723
antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents.the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ...200616869486
role of type ii topoisomerase mutations and acrab efflux pump in fluoroquinolone-resistant clinical isolates of proteus mirabilis.we conducted a study to determine the role played by amino acid mutations in dna gyrase and topoisomerase iv, and the acrab efflux pump in resistance to fluoroquinolones in clinical isolates of proteus mirabilis.200616870650
systemic absorption of gentamicin nasal irrigations.to determine if gentamicin nasal irrigation is systemically absorbed, and to identify any ototoxic side effects related to its use.200616871924
hemihyperplasia syndromes.hemihyperplasia is a heterogenous group of disorders characterized by asymmetric limb growth. there is considerable confusion regarding their classification and ascertainment into various syndromes. we tried to look into the various aspects of hemihyperplasia syndromes.200616877856
nutritive value, antioxidant and antimicrobial properties of struchium sparganophora leaves.struchium sparganophora leaf is a green leafy vegetable popularly used in soup preparations in processed and unprocessed forms in nigeria. fresh leaves of s. sparganophora were soaked overnight to remove its bitterness, before determination of the nutrient and antinutrient content, while sun-dried s. sparganophora leaves were extracted with rectified spirit. the antioxidant and antimicrobial properties of the extracts were subsequently determined. the result of the study revealed that the fresh ...200616822216
pharmacokinetic and pharmacodynamic aspects of antimicrobial agents for the treatment of uncomplicated urinary tract infections.uncomplicated urinary tract infections (uti) are treated with beta-lactams, co-trimoxazole, quinolones and fosfomycin tromethamine. due to increasing resistance of causative pathogens, antibiotics should be used by considering their pharmacodynamic and pharmacokinetic characteristics. beta-lactams have time-dependent activity and should not be used once-daily. co-trimoxazole should be restricted due to increasing chemoresistance. fluoroquinolones play a primary role in the treatment of serious a ...200616829051
hospital acquired urinary tract infections in urology departments: pathogens, susceptibility and use of antibiotics. data from the pep and peap-studies.data from two internet-based studies on nauti in hospitalized urological patients are presented together: the pan european prevalence (pep) study, which was a 1-day prevalence study in november 2003; and the pan euro-asian prevalence (peap) study, which was carried out in november 2004. overall, 93 and 101 hospitals from the two studies, respectively, completed the hospital questionnaires and provided patient information for the present study. nauti was diagnosed according to the centres for dis ...200616829052
urinary tract infection caused by fluoroquinolone- and cephem-resistant enterobacteriaceae.in 2001, fluoroquinolone-resistant escherichia coli isolates emerged in japan in patients with uncomplicated urinary tract infection (uti), and accounted for ca. 8% of isolates. this is a worldwide occurrence as reported by the eco.sens study. the number of fluoroquinolone- and cephem-resistant enterobacteriaceae isolates from patients with complicated uti is increasing. most cephem-resistant isolates of e. coli, klebsiella pneumoniae and proteus mirabilis produce extended-spectrum beta-lactamas ...200616829056
[wound care management of ulceration in a palliative care patient].the wound care management of a palliative care patient with sinusitis pinoidalis is documented. the treatment is validated by the expert's opinion. there is only little evidence-based treatment of such a lesion. in our experience, photo documentation is helpful in decision-making during the treatment process. in addition, healing of larger defects seems to improve upon frequent and extensive liquid cleansing.200616830251
structure of the glycerol phosphate-containing o-polysaccharides and serological studies of the lipopolysaccharides of proteus mirabilis ccug 10704 (oe) and proteus vulgaris tg 103 classified into a new proteus serogroup, o54.o-polysaccharides were obtained from the lipopolysaccharides of proteus mirabilis ccug 10704 (oe) and proteus vulgaris tg 103 and studied by chemical analyses and one- and two-dimensional (1)h and (13)c nuclear magnetic resonance spectroscopy, including rotating-frame nuclear overhauser effect spectroscopy, h-detected (1)h,(13)c heteronuclear single-quantum spectroscopy and (1)h,(31)p heteronuclear multiple-quantum spectroscopy experiments. the proteus mirabilis oe polysaccharide was found to ha ...200616831214
[a puzzle of the physician and the patient]. 200616835961
[clinical effect of continuous infusion of meropenem on bacterial pneumonia in the elderly].we studied the clinical effect of continuous infusion over 24 hours of meropenem (mepm) on bacterial pneumonia in the elderly (over 65). the subjects were 26 patients (community-acquired pneumonia: moderate, n = 9; severe, n= 4; hospital-acquired pneumonia: group iii, n = 13) whose performance status was 3 or 4. mepm 1.0g/day was infused continuously for 7-14 days, and its clinical efficacy, bacteriological efficacy, and side effects were examined prospectively. it was effective in 23 of the 26 ...200616841713
the mechanism of plasmid curing in bacteria.bacterial plasmids have a major impact on metabolic function. lactose fermentation of e. coli or hemolysin b transporter expressed by the plasmids that carry these respective genes could be readily obviated by heterocyclic compounds that readily bind to plasmid dna. these compounds could also reverse the resistance to antibiotics of e. coli, enterobacter, proteus, staphylococcus and yersinia strains by eliminating plasmids. however, the frequency and extent of this effect was significantly less ...200616842214
effectiveness of flouroquinolone therapy for gram-negative infections within the intensive care unit setting. 200616750952
antimicrobial and toxicological profile of the new biocide akacid plus.akacid plus is a new member of the polymeric guanidine family of disinfectants. it was especially developed to enhance the antimicrobial activity of this class with significantly less toxicity. the in vitro activity of akacid plus compared with chlorhexidine digluconate and mupirocin was tested against a total of 369 recent clinical isolates.200616751199
[proteus mirabilis community-acquired pneumonia]. 200616752451
[prevalence of esbl strains in urinary tract infections in children in 1996 and 2004].ethiological agents in urinary tract infections and prevalence of bacterial strains producing extended spectrum of beta-lactamases isolated from urine of children with urinary tract infections were compared. patients were hospitalized in department of pediatry, pediatric gastroenterology and oncology of medical university of gdańsk in 1996 and 2004. seventy bacterial strains isolated from urine in 1996 and 113 strains isolated in 2004 were analyzed. in both years enterobacteriaceae were the most ...200616758740
[a young patient with portal and mesenteric vein thrombosis and proteus syndrome].in the 26th year of life a young woman suffered a portal and mesenteric thrombosis followed by portal hypertension with splenomegaly, esophageal varices and pancytopenia. after splenorenal shunt surgery and splenectomy hematologic parameters resolved rapidly. also, she was suffering of proteus syndrome, which is an extremely rare and sporadic hamartomatous disorder characterized by a variety of cutaneous and subcutaneous tumors including vascular malformations, several types of nevi, partial gig ...200616758842
colonization of sitka spruce stumps by decay-causing hymenomycetes in paired inoculations.sitka spruce stumps were inoculated with decay fungi using colonized sawdust or dowel inoculum to investigate colonization in paired combinations. estimates of domain sizes were made in the top 15cm of stump after 13-14 or 21-23 months with sawdust or dowel inoculations, respectively. none of the co-inoculated species prevented colonization by heterobasidion annosum; sapwood colonization by resinicium bicolor may limit growth of h. annosum colonies out of heartwood, reducing the incidence of dis ...200616814536
characterisation of prototype nurmi cultures using culture-based microbiological techniques and pcr-dgge.undefined nurmi-type cultures (ntcs) have been used successfully to prevent salmonella colonisation in poultry for decades. such cultures are derived from the caecal contents of specific-pathogen-free birds and are administered via drinking water or spray application onto eggs in the hatchery. these cultures consist of many non-culturable and obligately anaerobic bacteria. due to their undefined nature it is difficult to obtain approval from regulatory agencies to use these preparations as direc ...200616814892
structure and evolution of the mitochondrial control region and flanking sequences in the european cave salamander proteus anguinus.the european cave salamander proteus anguinus laurenti 1768 is one of the best-known subterranean animals, yet its evolutionary history and systematic relationships remain enigmatic. this is the first comprehensive study on molecular evolution within the taxon, using an mtdna segment containing the control region (cr) and adjacent sequences. two to seven tandem repeats of 24-32 bp were found in the intergenic spacer region (vntr1), and three, four or six repeats, 59-77 bp each, in the 3' end of ...200616764998
emergence of proteus mirabilis carrying the bla metallo-beta-lactamase gene.seven genetically related proteus mirabilis clinical isolates from a hospital in thessaloniki, greece, exhibited decreased susceptibility to imipenem and carried a bla(vim-1) metallo-beta-lactamase gene. pcr mapping revealed that bla(vim-1) was part of a class 1 integron that was probably located in the chromosome and also included the aaca7, dhfr and aada genes. this is the first description of the bla(vim-1) metallo-beta-lactamase gene in p. mirabilis.200616774572
improving the accuracy of protein secondary structure prediction using structural alignment.the accuracy of protein secondary structure prediction has steadily improved over the past 30 years. now many secondary structure prediction methods routinely achieve an accuracy (q3) of about 75%. we believe this accuracy could be further improved by including structure (as opposed to sequence) database comparisons as part of the prediction process. indeed, given the large size of the protein data bank (>35,000 sequences), the probability of a newly identified sequence having a structural homol ...200616774686
biofilm formation in an ice cream plant.the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ...200616779628
[extended-spectrum-beta-lactamase-producing proteus mirabilis: laboratory-based surveillance in cooperation with 12 clinical laboratories in the kinki region of japan].we studied 247 strains of proteus mirabilis collected during the 6 months from november 2003 to april 2004 from 12 clinical laboratories in the kinki region of japan for the production of extended-spectrum beta-lactamase (esbl). eighteen strains (7.3%) showed mics for cefpodoxime of > or = 2 microg/ml and 13 strains (5.2%) were positive for the double-disk synergy test. susceptibility depended on genotype. mics for cefepime, cefozopran, and cefpirome were high (> or = 8 microg/ml), and that for ...200616780129
synthesis and in vitro antimicrobial studies of medicinally important novel n-alkyl and n-sulfonyl derivatives of 1-[bis(4-fluorophenyl)-methyl]piperazine.a series of novel substituted 1-[bis(4-fluorophenyl)-methyl]piperazine derivatives (4a-g) and (5h-m) have been synthesized. the synthesized compounds were characterized by ir and 1h nmr. all the synthesized compounds were evaluated in vitro for their efficacy as antimicrobial agents against representative strains of gram-positive (staphylococcus aureus atcc 25953, streptococcus pneumoniae atcc 49619, bacillus cereus 11778, and bacillus subtilis 6051) and gram-negative bacteria (escherichia coli ...200616784863
the comparison of pyrosequencing molecular gram stain, culture, and conventional gram stain for diagnosing orthopaedic infections.we have developed a combined real-time pcr and pyrosequencing assay that successfully differentiated the vast majority of gram-positive and gram-negative bacteria when bacterial isolates were tested. the purpose of this study was to evaluate this assay on clinical specimens obtained from orthopedic surgeries, and to prospectively compare the results of "molecular gram stain" with culture and conventional direct gram stain. forty-five surgical specimens were obtained from patients who underwent o ...200616788984
tigecycline: a new glycylcycline antimicrobial agent.the pharmacology, spectrum of activity, pharmacokinetics, clinical efficacy, adverse events, dosage and administration, drug interactions, and place in therapy of tigecycline are reviewed.200616790575
structure of escherichia coli tryptophanase.pyridoxal 5'-phosphate (plp) dependent tryptophanase has been isolated from escherichia coli and its crystal structure has been determined. the structure shares the same fold with and has similar quaternary structure to proteus vulgaris tryptophanase and tyrosine-phenol lyase, but is found in a closed conformation when compared with these two enzymes. the tryptophanase structure, solved in its apo form, does not have covalent plp bound in the active site, but two sulfate ions. the sulfate ions o ...200616790938
capillary isoelectric focusing of microorganisms in the ph range 2-5 in a dynamically modified fs capillary with uv detection.the isoelectric points of many microbial cells lie within the ph range spanning from 1.5 to 4.5. in this work, we suggest a cief method for the separation of cells according to their isoelectric points in the ph range of 2-5. it includes the segmental injection of the sample pulse composed of the segment of the selected simple ampholytes, the segment of the bioanalytes and the segment of carrier ampholytes into fused silica capillaries dynamically modified by poly(ethylene glycole). this polymer ...200616791563
a simple phenotypic method for differentiation between acquired and chromosomal ampc beta-lactamases in escherichia coli.screening methods for the detection of plasmid-mediated ampc beta-lactamases are technically demanding. the purpose of this study was to assess screening methods for the detection of these enzymes in clinical isolates of escherichia coli, klebsiella pneumoniae and proteus mirabilis.200616792938
gram-negative bacteria from patients seeking medical advice in stockholm after the tsunami catastrophe.microbiological cultures from 229 patients seeking medical advice in stockholm after the tsunami catastrophe of december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative bacilli were the most common findings from wound cultures. common human pathogens such as escherichia coli, proteus species, klebsiella spp., and pseudomonas aeruginosa were isolated. more rare species of gram-negative bacilli, e.g. myroides odoratus, sp ...200616798691
proteus mirabilis spondylodiscitis complicating a urinary tract infection.infectious spondylodiscitis results from local or haematogenous infection by microorganisms of intervertebral disc spaces, vertebral bodies and surrounding structures. haematogenous invasion may follow urosepsis. we report on a case of septic spondylodiscitis following urosepsis with proteus mirabilis, a frequently isolated microorganism in urinary tract infections but rarely in spondylodiscitis.200616798719
the ability of flagellum-specific proteus vulgaris bacteriophage pv22 to interact with campylobacter jejuni flagella in culture.there has been a recent resurgent interest in bacteriophage biology. research was initiated to examine campylobacter jejuni-specific bacteriophage in the russian federation to develop alternative control measures for this pathogen.200616803630
proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice.proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing an important role in urinary tract infections (utis). in this study, a collection of p. mirabilis isolates obtained from clinical and non-clinical sources was analysed in order to determine a possible correlation between origin, virulence factors and in vivo infectivity. isolates were characterized in vitro, assessing several virulence properties that had been previously associated with p. mirabi ...200616804188
mapping bacterial surface population physiology in real-time: infrared spectroscopy of proteus mirabilis swarm colonies.we mapped the space-time distribution of stationary and swarmer cells within a growing proteus mirabilis colony by infrared (ir) microspectroscopy. colony mapping was performed at different positions between the inoculum and the periphery with a discrete microscope-mounted ir sensor, while continuous monitoring at a fixed location over time used an optical fiber based ir-attenuated total reflection (atr) sensor, or "optrode." phenotypes within a single p. mirabilis population relied on identific ...200616808858
[heat shock proteins of freshwater protists and their involvement in adaptation to changes in the environmental salinity].changes in the level of heat shock proteins (hsp) in cells of freshwater protists, amoebae amoeba proteus and ciliates paramecium jenningsi, in response to changes in the environmental salinity were investigated. changes in salinity levels were considered as a stress factor. the immunoblotting method revealed a polypeptide antigen cross-reacting with antibodies against bovine hsp70 in total protein extracts of both intact cells and cells subjected to salinity stress. the same polypeptide antigen ...200616893060
surgical site infections in day surgery settings.because major complications are rare in day surgery, minor morbidities such as surgical site infections, which become evident only after patient discharge, have a major impact on the perceived quality of care. we performed an audit of such infections over a oneyear period.200616895493
new trends in prosthesis infection in cardiovascular surgery.adult cardiovascular surgery entails, in many cases, the use of some kind of prosthesis. among the potential complications, prosthetic device infection is one of the most devastating in incidence, as well as in prognosis and damage to surrounding tissues.200616895504
a case of proteus syndrome (elephant man). 200616898038
[microorganisms isolated from middle ear cultures and their antibacterial susceptibility in patients with chronic suppurative otitis media].this study sought to determine causative microorganisms of chronic suppurative otitis media and their antibacterial susceptibility.200616905907
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2004). i. susceptibility distribution].the bacterial strains isolated from 490 patients diagnosed as having urinary tract infections (utis) in 14 institutions in japan were collected between august 2004 and july 2005. the susceptibilities of them to many kinds of antimicrobial agents were measured. of them, 577 strains were estimated as causative bacteria and used for the measurement. the strains consisted of 156 gram-positive bacterial strains (27.0%) and 421 gram-negative bacterial strains (73.0%). against staphylococcus aureus, ar ...200616913404
how long do nosocomial pathogens persist on inanimate surfaces? a systematic review.inanimate surfaces have often been described as the source for outbreaks of nosocomial infections. the aim of this review is to summarize data on the persistence of different nosocomial pathogens on inanimate surfaces.200616914034
pcr for the identification and differentiation of histomonas meleagridis, tetratrichomonas gallinarum and blastocystis spp.in the present investigation pcr assays were developed for the rapid detection and differentiation of two poultry flagellates: histomonas meleagridis and tetratrichomonas gallinarum as well as the protozoan microorganism: blastocystis spp. the nucleotide sequences of the small subunit ribosomal rnas were used for primer construction obtaining fragments which vary in size for each microorganism. the established pcrs were able to detect dna obtained from one microorganism of t. gallinarum and blas ...200616920265
a comparison of antimicrobial susceptibility profile of urinary pathogens for the years, 1999 and 2003.urinary tract infection is a common condition worldwide; responsible for significant morbidity in both hospitalized and community patients. the laboratory records, for microbial isolates of infected urine and their susceptibility profiles for the years 1999 and 2003 were retrospectively reviewed and compared. in 2003, there was a significant decline in recovery ofcitrobacter spp compared to 1999. conversely, the proportion of k pneumoniae, e coli and enterococci increased dramatically in 2003, i ...200616921702
fishmeal extract bile salt lactose agar--a differential medium for enteric bacteria.fishmeal extract bile salt lactose agar (febla), a new differential medium for enteric bacteria was developed and evaluated for its ability to grow and differentiate lactose fermenters (lf) from non-lactose fermenters (nlf) in comparison with macconkeys agar. performance of febla was at par with the latter. on febla medium, the contrast between lf and nlf colonies was pronounced and klebsiella pneumoniae produced more mucoid colonies than on macconkeys agar (hi media). unlike macconkeys agar, a ...200616924840
emerging resistance to carbapenems in a tertiary care hospital in north india.carbapenems are beta-lactam antibiotics, presently considered as most potent agents for treating multi-drug resistant gram-negative bacilli infections. in india carbapenems available for use are meropenem and imipenem, introduced recently. resistance to these has been reported in a few bacteria especially pseudomonas spp. we therefore retrospectively evaluated the antibiotic susceptibility pattern to these agents amongst various clinical isolates in a tertiary care hospital in north india.200616926463
extended-spectrum beta-lactamases in taiwan: epidemiology, detection, treatment and infection control.extended-spectrum beta-lactamases (esbls) efficiently hydrolyze extended-spectrum beta-lactams such as cefotaxime, ceftriaxone, ceftazidime, and aztreonam. esbls are most often plasmid-mediated. in taiwan, the prevalence of esbls in bacteria has risen, ranging from 8.5 to 29.8% in klebsiella pneumoniae and 1.5 to 16.7% in escherichia coli isolates. the most prevalent types of esbls are shv-5, shv-12, ctx-m-3, and ctx-m-14 in isolates of k. pneumoniae and e. coli, with differences between institu ...200616926972
1h nmr spectroscopy in the diagnosis of klebsiella pneumoniae-induced urinary tract infection.the (1)h nmr spectroscopic method is suggested and its utility is demonstrated for the diagnosis of klebsiella pneumoniae (k. pneumoniae) in urinary tract infection (uti). k. pneumoniae have the specific property of metabolizing glycerol to 1,3-propanediol (1,3-pd), acetate, ethanol and succinate. the quantity of 1,3-pd produced correlates well with the viable bacterial count. other common bacteria causing uti (except for citrobacter frundii), such as escherichia coli (e. coli), pseudomonas aeru ...200616927393
comparison of susceptibility to antimicrobials of bacterial isolates from companion animals in a veterinary diagnostic laboratory in canada between 2 time points 10 years apart.the susceptibility to antimicrobials of bacterial species most frequently isolated from companion animals in a veterinary teaching diagnostic laboratory was evaluated retrospectively. a significant decrease between 1990-1992 and 2002-2003 was noted in the susceptibility of dog isolates to the following antimicrobials: escherichia coli to cephalothin (86% to 61%, p < 0.001); e. coli to ampicillin (85% to 67%, p < 0.001); proteus spp. to ampicillin (92% to 71%, p < 0.01); coagulase-positive staphy ...200616933555
assessment of pathogen frequency and resistance patterns among pediatric patient isolates: report from the 2004 sentry antimicrobial surveillance program on 3 continents.selecting empiric or directed therapy for pathogens isolated from pediatric patients can be problematic. many antimicrobial agents are not indicated for use in pediatric patients, and regional variations of resistance mechanisms have been reported. the purpose of this study was to analyze antimicrobial resistance patterns and pathogen occurrence rates in pediatric-aged patient infections on 3 continents using data from the sentry antimicrobial surveillance program. a total of 3537 clinical isola ...200616938419
effective prevention of microbial biofilm formation on medical devices by low-energy surface acoustic waves.low-energy surface acoustic waves generated from electrically activated piezo elements are shown to effectively prevent microbial biofilm formation on indwelling medical devices. the development of biofilms by four different bacteria and candida species is prevented when such elastic waves with amplitudes in the nanometer range are applied. acoustic-wave-activated foley catheters have all their surfaces vibrating with longitudinal and transversal dispersion vectors homogeneously surrounding the ...200616940055
mode of transposition and expression of 16s rrna methyltransferase gene rmtc accompanied by isecp1.a newly identified 16s rrna methyltransferase gene, rmtc, was accompanied by an isecp1 element at its 5' end. this isecp1 element, which contained a transposase gene, tnpa, provided a promoter activity for expression of the adjacent rmtc; and this structure enabled the rmtc gene to be transposed onto another plasmid in escherichia coli.200616940134
relevance of weil-felix test in diagnosis of scrub typhus in india.to study the relationship of weil-felix test and microimmunofluorescence test.200616941792
unusual fallopian tube neoplasm in a 10-year-old patient with proteus syndrome: a case report and review of the literature.we report a case of fallopian tube and peritoneal lesions resected from a 10-year-old girl with proteus syndrome. the papillary fallopian tube tumor expressed ca-125 and demonstrated negative staining for cea, cd15, calretinin, and ck 5/6. nondilated portions of fallopian tube exhibited evidence of an in situ component. this patient was also found to have peritoneal mesothelial hyperplasia as well as cystic peritoneal lesions of mesothelial origin, with positive calretinin staining. this case pr ...200616944982
visuomotor tracking related to attention-deficit hyperactivity disorder (adhd).deficient visuomotor tracking in children with attention-deficit hyperactivity disorder (adhd) has been described, but the specific influence of attention on this deficit has not yet been elucidated. the present study compares visuomotor tracking under different conditions of attentional loading in children with adhd with that of age-matched controls. a computerized visuomotor attentional tracking test that incorporated several levels of distraction was administered to 13 typical children. the s ...200616948935
the dps gene of symbiotic "candidatus legionella jeonii" in amoeba proteus responds to hydrogen peroxide and phagocytosis.to survive in host cells, intracellular pathogens or symbiotic bacteria require protective mechanisms to overcome the oxidative stress generated by phagocytic activities of the host. by genomic library tagging, we cloned a dps (stands for dna-binding protein from starved cells) gene of the symbiotic "candidatus legionella jeonii" organism (called the x bacterium) (dps(x)) that grows in amoeba proteus. the gene encodes a 17-kda protein (pi 5.19) with 91% homology to dps and dna-binding ferritin-l ...200616950918
a female with hemihypertrophy and chylous ascites - klippel-trenaunay syndrome or proteus syndrome: a diagnostic dilemma.asymmetric overgrowth has many differential diagnoses with considerable overlap, posing a diagnostic dilemma. the presence of chylous ascites, though unreported, might be expected as a manifestation of overgrowth syndromes with lymphatic involvement. we present a patient with hemihypertrophy who presented with chylous ascites at birth.200616957479
analysis on antimicrobial resistance of clinical bacteria isolated from county hospitals and a teaching hospital.the distinction of antimicrobial resistance of clinical bacteria isolated from county hospitals and a teaching hospital was investigated. disc diffusion test was used to study the antimicrobial resistance of isolates collected from county hospitals and a teaching hospital. the data was analyzed by whonet5 and spss statistic software. a total of 655 strains and 1682 strains were collected from county hospitals and a teaching hospital, respectively, in the year of 2003. the top ten pathogens were ...200616961303
occurrence of qnra-positive clinical isolates in french teaching hospitals during 2002-2005.bacteria harbouring the novel qnra plasmid-mediated mechanism of quinolone resistance have been described in different countries, but the frequency of their occurrence has not been investigated. in total, 1,468 clinical isolates of enterobacteriaceae with quinolone resistance or extended-spectrum beta-lactamase (esbl) phenotypes were collected from eight teaching hospitals in france during 2002-2005 and screened for qnra. overall, 28 isolates (22 enterobacter cloacae, three klebsiella pneumoniae ...200616961639
the catalytic machinery of chondroitinase abc i utilizes a calcium coordination strategy to optimally process dermatan sulfate.the chondroitinases are bacterial lyases that specifically cleave chondroitin sulfate and/or dermatan sulfate glycosaminoglycans. one of these enzymes, chondroitinase abc i from proteus vulgaris, has the broadest substrate specificity and has been widely used to depolymerize these glycosaminoglycans. biochemical and structural studies to investigate the active site of chondroitinase abc i have provided important insights into the catalytic amino acids. in this study, we demonstrate that calcium, ...200616964974
synthesis, structure and biological activity of cobalt(ii) and copper(ii) complexes of valine-derived schiff bases.we have synthesized two cobalt(ii) 2 and copper(ii) 3 complexes of valine-derived schiff bases. the obtained complexes were characterized by elemental analysis, ft-ir and x-ray diffraction. biological studies of complexes 2 and 3 had been carried out in vitro for antimicrobial activity against gram-positive, gram-negative bacteria and human pathogenic fungi. compound 3 was proven to be a broad spectrum agent, showed a significant inhibition of the growth of gram-positive bacteria (staphylococcus ...200616965817
ft-ir microspectroscopy for early identification of some clinically relevant pathogens.to investigate the potentials and limitations of fourier transform-infrared (ft-ir) microspectroscopy as a tool to identify, at the level of microcolonies, pathogenic bacteria frequently isolated in the clinical environment.200616968290
a novel gene involved in regulating the flagellar gene cascade in proteus mirabilis.in this study, we identified a transposon insertion in a novel gene, designated disa, that restored swarming motility to a putrescine-deficient spea mutant of proteus mirabilis. a null allele in disa also increased swarming in a wild-type background. the disa gene product was homologous to amino acid decarboxylases, and its role in regulating swarming was investigated by examining the expression of genes in the flagellar cascade. in a disa mutant background, we observed a 1.4-fold increase in th ...200616980463
[evaluation of anti-blastoma effects of tetravaccine in combination with chemotherapy].the present work discusses methods of immune-correction and immune-rehabilitation of oncological patients using specific bacterial polyvalent vaccines. with this purpose the tetra-vaccine (staphilococcus-proteus-clepsiela-escherichia) has been used. experiments have been carried out on white rats and mice with sarcoma c-45. for chemotherapy of malignant tumors the following remedies were used: cyclophosphan, vincristin and adriablastin. the vaccine and chemotherapy were applied in both ways, sep ...200616980764
urinary bactericidal activity of extended-release ciprofloxacin (1,000 milligrams) versus levofloxacin (500 milligrams) in healthy volunteers receiving a single oral dose.twelve volunteers received a single oral dose of 1,000 mg extended-release (xr) ciprofloxacin versus 500 mg levofloxacin to assess urinary bactericidal titers (ubts) against common uropathogens. areas under ubt-time curves were significantly larger for proteus mirabilis with xr ciprofloxacin and for staphylococci with levofloxacin.200616982785
rapid onset of ulcerative typhlocolitis in b6.129p2-il10tm1cgn (il-10-/-) mice infected with helicobacter trogontum is associated with decreased colonization by altered schaedler's flora.infection with helicobacter trogontum, a urease-positive helicobacter isolated from subclinically infected rats, was evaluated in b6.129p2-il10(tm1cgn) (interleukin-10(-/-) [il-10(-/-)]) and c57bl/6 (b6) mice. in a first experiment, il-10(-/-) mice naturally infected with helicobacter rodentium had subclinical typhlocolitis but developed severe diarrhea and loss of body condition with erosive to ulcerative typhlocolitis within 1 to 3 weeks of experimental infection with h. trogontum. a second ex ...200616982822
multiple bacterial species reside in chronic wounds: a longitudinal study.the aim of the study was to investigate the bacterial profile of chronic venous leg ulcers and the importance of the profile to ulcer development. patients with persisting venous leg ulcers were included and followed for 8 weeks. every second week, ulcer samples were collected and the bacterial species present were identified. more than one bacterial species were detected in all the ulcers. the most common bacteria found were staphylococcus aureus (found in 93.5% of the ulcers), enterococcus fae ...200616984578
[development of a gold-immunochromatography test for rapid detecting e. coli o157].to develop a method for rapid detecting escherichia coli (e. coli) o157 on site.200616986519
antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents.the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ...200616989308
diabetic foot ulcers in cameroon: can microflora prevalence inform probabilistic antibiotic treatment?to determine the clinical features, regularly associated microorganisms and their susceptibility to antibiotics, and the clinical outcomes of foot ulcers in patients with diabetes at the yaoundé central hospital, cameroon.200617001946
phytochemical and antimicrobial study of an antidiabetic plant: scoparia dulcis l.the antimicrobial and antifungal effects of different concentrations of chloroform/methanol fractions of scoparia dulcis were investigated. the isolated fractions were tested against different bacteria like salmonella typhii, staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosa, and proteus vulgaris and fungal strains such as alternaria macrospora, candida albicans, aspergillus niger, and fusarium oxysporum. the isolated fractions exhibited significant antimicrobial ...200617004904
inhibition of swarming and virulence factor expression in proteus mirabilis by resveratrol.resveratrol (3,5,4-trihydroxy-trans-stilbene) is a phytoalexin compound with anti-inflammatory and antioxidant activities. the effect of resveratrol on swarming and virulence factor expression of proteus mirabilis, an important pathogen infecting the urinary tract, was determined on swarming agar plates with and without the compound. bacteria harvested at different times were assayed for cell length and the production of flagella, haemolysin and urease. resveratrol inhibited p. mirabilis swarmin ...200617005777
phytochemisty and spermatogenic potentials of aqueous extract of cissus populnea (guill. and per) stem bark.in vivo clinical trials involving the administration of crude extracts of cissus populnea to male subjects (normospermic, oligospermic, and azoopermic) in a 72-day study revealed that continuous exposure of the subjects to the extracts over this period did not significantly (p < or = 0.05) alter sperm count, morphology, motility, or volume. antimicrobial screening of the extract against some selected microbial isolates secondarily implicated in male infertility revealed total inactivity against ...200617370009
an in vitro assessment of the antibacterial effect of garlic (allium sativum) on bacterial isolates from wound infections.garlic (allium sativum) has come to be seen as an all rounded treatment for preventing wound infection, common cold, malaria, cough and lung tuberculosis, hypertension, sexually transmitted diseases, mental illness, kidney diseases, liver diseases, asthma, diabetes.200617370439
[evaluation of vitek 2 performance for identifying extended spectrum beta-lactamases in enterobacteriaceae "other than escherichia coli, proteus mirabilis and klebsiella spp"].production of beta-lactamases is the main resistance mechanism of gram-negative bacteria against beta-lactam antibiotics. extended spectrum beta-lactamases (esbls) have the ability to hydrolyze a broader spectrum of beta-lactam drugs. hence rapid, accurate detection of this resistance mechanism is extremely important to guide proper patient antimicrobial therapy. these enzymes are most commonly produced by klebsiella spp. and escherichia coli, but may also occur widely in other gram-negative bac ...200617380089
perinephric abscess secondary to a staghorn calculus presenting as a subcutaneous abscess.perinephric abscesses often present with flank pain and signs of sepsis, and are known to discharge to the skin. we report a case of a young patient presenting with a 48-hour history of painful swelling in her right renal angle following a urinary tract infection and no overt signs or symptoms of sepsis. plain abdominal films showed a perinephric abscess secondary to a staghorn calculus in the upper pole of the right kidney. on incision and drainage, 700 ml of pus was drained freely. the patient ...200617324312
spontaneous bacterial peritonitis causing serratia marcescens and proteus mirabilis ventriculoperitoneal shunt infection. case report.the authors report their experience treating a polymicrobial ventriculoperitoneal (vp) shunt infection in a developmentally delayed 21-year-old woman. cerebrospinal fluid (csf) cultures grew serratia marcescens and proteus mirabilis. on admission and throughout her hospitalization, results of physical examination of her abdomen were normal, and radiographic studies showed no evidence of bowel perforation or pseudocyst formation. contrast-enhanced computed tomography of the abdomen revealed a sma ...200617219841
more than just a bump: the hamartoma syndromes.the hamartoma syndromes detailed in this review are just a few examples of the many genodermatoses now known to be associated with uncontrolled tumor proliferation secondary to mutations in tumor suppressor genes. knowledge gained through the study of these syndromes has not only improved our understanding of patients afflicted with such conditions, but has also led to significant insight into the important role tumor suppressor genes play in preventing tumor formation and in carcinogenesis. as ...200617249301
identification and quantitation of usnic acid from the lichen usnea species of anatolia and antimicrobial activity.six species of lichens, such as usnea florida, usnea barbata, usnea longissima, usnea rigida, usnea hirta and usnea subflorida, were collected from different areas of anatolia (district of antalya, karabük, qankiri, giresun and trabzon) in turkey. their usnic acid amounts in acetone extracts were determined by hplc. in addition, antimicrobial activities of these extracts were determined against escherichia coli (atcc 35218), enterococcus faecalis (rskk 508), proteus mirabilis (pasteur ens. 235), ...200617294685
calcium sulphate delivery system with tobramycin for the treatment of chronic calcaneal osteomyelitis.the case of a 24-year-old woman with pseudomonas aeruginosa and proteus mirabilis chronic calcaneal osteomyelitis is presented. extensive debridement of the necrotic bone and application of tobramycin-loaded polymethylmethacrylate beads was performed and ciprofloxacin was given post-operatively. three months later, laboratory tests, including complete blood cell count, erythrocyte sedimentation rate and c-reactive protein, were normal. at this time, extensive surgical debridement and filling of ...200617295005
[antagonistic effect of bacteriocinogenic lactobacillus acidophilus on klebsiella pneumoniae, citrobacter freundii and proteus mirabilis cells].data of the ultrastructural cellular changes of conditionally pathogenic enterobacteria, including k. pneumoniae, c. freundii and p. mirabilis cells impacts to bacteriocin-producing l. acidophilus are presented. enterobacteria in response to the bacteriocinogenic effect of lactobacilli are manifestated by expressive destructions of sensitive to pore formation bacteriocin cells. various morphological types of enterobacteria cells with increase of involution, lysing and resting forms are revealed. ...200617297872
[pyrazinamidase activity of bacteria from enterobacteriaceae family as characteristic for taxonomy].pyrazinamidase activity in 330 strains of bacteria from enterobacteriaceae family (14 genus, 27 species) has been assessed. pyrazinamidase activity detected in species from following genuses: citrobacter, escherichia, klebsiella, kluyvera, morganella, providencia, raourtella, salmonella, shigella, and also in proteus mirabilis, and nonpathogenic serovars of yersinia enterocolitica, y. frederiksenii. pirasinamidase was absent in serratia (s. marcescens, s. liguefaciens), hafnia alvei, p. vulgaris ...200617297889
[bacteriological evaluation in chronic otitis media].in chronic otitis media bacterial infection is often a cause of exacerbation in the clinical course and failure in treatment. the aim of the study was to investigate the incidence and profile of the aerobic bacterial flora in chronic otitis media and to determine the drug susceptibility of selected isolated bacteria.200617263251
in vivo evaluation of teicoplanin- and calcium sulfate-loaded pmma bone cement in preventing implant-related osteomyelitis in rats.the objective of this study was to evaluate the efficacy of teicoplanin- and calcium sulphate-loaded polymethylmethacrylate (pmma) bone cements in preventing experimental implant-related osteomyelitis in rats. four groups of antibiotic-loaded rods were prepared and were implanted into the lateral condylus of the rat femur after inoculation of staphylococcus aureus. the effectiveness of these were assessed microbiologically, radiographically, and histopathologically. radiographic evaluation revea ...200617267341
pattern and multiple drug resistance of bacterial pathogens isolated from wound infection at university of gondar teaching hospital, northwest ethiopia.antibiotics resistance among bacteria is a worldwide problem. the situation in developing countries like ethiopia is particularly serious. since the presence of drug resistant bacteria in the environment are threat for public health, up-to-date information on local pathogens and drug sensitivity pattern is very crucial to treat patients. the aim of this study is to assess the pattern of bacterial pathogens from wound infection and their susceptibility to common antibiotics.200617447374
mini-tn7 insertion in bacteria with secondary, non-glms-linked atttn7 sites: example proteus mirabilis hi4320.we previously constructed a series of mini-tn7 chromosome integration vectors that, when provided only with the site-specific transposition machinery, generally transpose to a naturally evolved, neutral atttn7 site that is located 25-bp downstream of the glms gene. here we provide a protocol for application of the mini-tn7 system in proteus mirabilis as an example of a bacterium with a secondary atttn7 site that is not linked to glms but, in this case, located in the carab operon. the procedure ...200617406229
microbiologic profile of flexible endoscope disinfection in two brazilian hospitals.[corrected] endoscopes are routinely used in hospitals and clinics of the world and they can be potential sources of cross-infection when the decontamination process is unsuitable200617406750
survival of proteus mirabilis o3 (s1959), o9 and o18 strains in normal human serum (nhs) correlates with the diversity of their outer membrane proteins (omps).urinary tract infections are frequently caused by proteus mirabilis strains. in the previous studies there were defined the complete structures of o-polysaccharide parts of lipopolysaccharides from strains: p. mirabilis o3 (s1959), p. mirabilis o9 and p. mirabilis o18. in the present study it was investigated bactericidal effect of normal human serum (nhs) to p. mirabilis strains. we also focused on the diversity of outer membrane proteins (omps) being separated on a gel isolated from tested str ...200617419294
a new antibacterial compound produced by an indigenous marine bacteria--fermentation, isolation, and biological activity.the use of microorganisms for biological purpose has become an effective alternative to control pathogens. a marine bacterium pseudomonas aeruginosa was isolated from eal fish of baluchistan coast of pakistan. this strain produced a bactericidal antibiotic against environmental and clinical isolates. in this study, we purified bactericidal antibiotic from the ethyl acetate extract of the cells of p. aeruginosa and analyzed its chemical structure. based on spectrometric analysis, this compound 1 ...200617393659
[biological hazards in air at municipal waste landfills].the aim of the study was to assess the microbiological air quality at the selected municipal waste landfills.200617533990
etiology of chronic prostatitis syndrome in patients treated at the university hospital for infectious diseases "dr. fran mihaljević" from 2003 to 2005.a total of 835 patients with symptoms of chronic prostatitis syndrome and no evidence of structural or functional lower genitourinary tract abnormalities were examined in a three year period at the outpatient department for urogenital infections, university hospital for infectious diseases "dr. fran mihaljević" zagreb, croatia. disease etiology was determined in 482 (57.72%) patients. chlamydia trachomatis was proved to be the causative pathogen in 161 patients, trichomonas vaginalis in 85, esch ...200617508488
antimicrobial activity against gram negative bacilli from yaounde central hospital, cameroon.antimicrobial resistance among bacteria pathogens is a world-wide issue. the antimicrobial susceptibility patterns of common pathogenic bacteria are essential to guide empirical and pathogen-specific therapy; unfortunately, these data are scarse in cameroon.200617604512
hyperoxaluria, hypocitraturia, hypomagnesiuria, and lack of intestinal colonization by oxalobacter formigenes in a cervical spinal cord injury patient with suprapubic cystostomy, short bowel, and nephrolithiasis.although urolithiasis is common in spinal cord injury patients, it is presumed that the predisposing factors for urinary stones in spinal cord injury patients are immobilization-induced hypercalciuria in the initial period after spinal injury and, in later stages, urine infection by urease-producing micro-organisms, e.g., proteus sp., which cause struvite stones. we describe a patient who sustained c-7 complete tetraplegia in a road traffic accident in 1970, when he was 16 years old. left ureter ...200617619709
plasmid determined resistance to quinolones in clinical isolates of gram-negative bacilli.a total of 166 strains of gram-negative bacilli comprising 65 klebsiella species, 22 escherichia coli, 39 pseudomonas aeruginosa, 20 proteus species, 18 other pseudomonas species and 2 salmonella typhi, were isolated from clinical specimens in the university college hospital, ibadan. antimicrobial susceptibility testing and plasmid profiling of the strains were done. ceftazidine had the highest antibacterial activity of 83.1% compared to the fluoroquinolones followed by ciprofloxacin (78.3%), pe ...200617722809
evaluation of antimicrobial activity of the stem bark of cylicodiscus gabunensis (mimosaceae).ethyl acetate (ea) extract of the stem bark of cylicodiscus gabunensis (cg) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: escherichia coli, klebsiella pneumoniae, shigella dysenteriae, shigella flexneri, morganella morganii, proteus vulgaris, proteus mirabilis, salmonella typhi, citrobacter freundii, enterobacter cloacae, enterobacter agglomerans, staphylococcus aureus, streptococcus feacalis, pseudomonas aeruginosa ...200620162076
production and characterisation of monoclonal antibodies specific for escherichia coli o157:h7.seven monoclonal antibodies (mabs) specific for escherichia coli o157:h7, one of the major causes of haemorrhagic colitis in humans, were produced by immunising balb/c mice with the strain e. coli o157:h7. these monoclonal antibodies do not cross-react with other bacteria such as salmonella enterica serovar typhimurium, e. coli o14, e. coli jm109, s. enterica serovar enteritidis, s. panama, s. saintpaul, s. derby, s. muenchen, s. bredeney, s. hadar, yersinia enterocolitica, proteus vulgaris, shi ...200620429059
antifungal and antibacterial activities of mexican tarragon (tagetes lucida).mexican tarragon (tagetes lucida cv. asteraceae: campanulatae) is an important, nutritious plant and an effective herbal medicine. seven coumarins, 7,8-dihydroxycoumarin (4), umbelliferone (7-hydroxycoumarin) (5), scoparone (6,7-dimethoxycoumarin) (7), esculetin (6,7-dihydroxycoumarin) (11), 6-hydroxy-7-methoxycoumarin (12), herniarin (7-methoxycoumarin) (13), and scopoletin (6-methoxy-7-hydroxycoumarin) (14), and three flavonoids, patuletin (18), quercetin (19), and quercetagetin (20), were iso ...200619127719
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