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[rapid detection of pseudomonas aeruginosa by the fluorescence quantitative pcr assay targeting 16s rdna].the 16s rdna specific primers were designed for rapid detection of pseudomonas aeruginosa (pa) by the fluorescence quantitative pcr (fq-pcr) assay, based upon multiple sequence alignment and phylogenetic tree analysis of the 16s rdnas of over 20 bacteria. after extraction of pa genomic dna, the target 16s rdna fragment was amplified by pcr with specific primers, and used to construct recombinant pmdt-pfr plasmid, the dilution gradients of which were subjected to the standard quantitation curve i ...200617037203
antimicrobial properties of erythromycin and colistin impregnated bone cement. an in vitro analysis.deep surgical site infection is a devastating consequence of total joint arthroplasty. the use of antibiotic impregnated bone cement is a well-accepted adjunct for treatment of established infection and prevention of deep orthopaedic infection. it allows local delivery of the antibiotic at the cement-bone interface and sustained release of antibiotic provides adequate antibiotic coverage after the wound closure. preclinical testing, randomised and clinical trials indicate that the use of antibio ...200617042224
antimicrobial activity of some ethnomedicinal plants used by paliyar tribe from tamil nadu, india.antimicrobial activity of 18 ethnomedicinal plant extracts were evaluated against nine bacterial strains (bacillus subtilis, staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa, ervinia sp, proteus vulgaris) and one fungal strain (candida albicans). the collected ethnomedicinal plants were used in folk medicine in the treatment of skin diseases, venereal diseases, respiratory problems and nervous disorders.200617042964
bactericidal activity of ertapenem against major intra-abdominal pathogens.treatment of intra-abdominal infections remains a challenge owing to their polymicrobial nature and associated mortality risk. treatment regimens must provide broad-spectrum coverage, including gram-positive and gram-negative aerobic and anaerobic bacteria of gastrointestinal origin. ertapenem is a long-acting 1-beta-methyl parenteral group 1 carbapenem antibiotic that has a broad antibacterial spectrum and once-daily dosing supported by clinical studies. it is active against gram-positive and g ...200617045463
[characteristics of war wound infection].war wounds are the most complex type of non-targeted injuries due to uncontrolled tissue damage of varied and multifold localizations, exposing sterile body areas to contamination with a huge amount of bacteria. wound contamination is caused by both the host microflora and exogenous agents from the environment (bullets, cloth fragments, dust, dirt, water) due to destruction of the host protective barriers. war wounds are the consequence of destructive effects of various types of projectiles, whi ...200617048790
[development of beta-lactamase resistance in enterobacteria].enterobacteria produce elementary chromosomal enzymes, beta-lactamases of class a: tem and shv (escherichia coli, klebsiella pneumoniae). these can give rise to plasmid-coded broad-spectrum beta-lactamases (esbl) discovered in 1980 (e. coli, k. pneumoniae, enterobacter cloacae). the first cefotaximase (ctx-m, men-1) was reported in europe in 1990. this enzyme is far more active against cefotaxime than against ceftazidime and aztreonam. chromosomal hyperpoduction of k1 beta-lactamase differs from ...200617051471
human gamma delta t cells and tumor immunotherapy.human vgamma2vdelta2 t cells recognize nonpeptide antigens derived from pathogenic microbes in a tcr-dependent manner, such as pyrophosphomonoester compounds from mycobacteria and malaria parasite and alkyl amines from proteus, suggesting that this subset of gamma delta t cells is involved in infectious immunity. the precise recognition mechanism has been delineated using a site-directed mutagenesis strategy based on crystal structure of gamma delta tcr. on the other hand, several lines of evide ...200617058804
metalloantibiotics: synthesis, characterization and in-vitro antibacterial studies on cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes with cloxacillin.the synthesis and characterization of cloxacillin (clox) complexes with divalent metal ions [co (ii), cu (ii), ni (ii) and zn (ii)] is described. the nature of bonding of the chelated cloxacillin and the structures of the metal complexes have been elucidated on the basis of their physical and spectroscopic data. in all the complexes, the cloxacillin acts as a uninegatively charged bidentate ligand with coordination involving the carboxylate-o and endocyclic-n of the beta-lactam ring. the new com ...200617059178
[a young patient with portal and mesenteric vein thrombosis and proteus syndrome].in the 26th year of life a young woman suffered a portal and mesenteric thrombosis followed by portal hypertension with splenomegaly, esophageal varices and pancytopenia. after splenorenal shunt surgery and splenectomy hematologic parameters resolved rapidly. also, she was suffering of proteus syndrome, which is an extremely rare and sporadic hamartomatous disorder characterized by a variety of cutaneous and subcutaneous tumors including vascular malformations, several types of nevi, partial gig ...200616758842
clinical and economic impact of bacteremia with extended- spectrum-beta-lactamase-producing enterobacteriaceae.we studied outcomes of extended-spectrum beta-lactamase (esbl) production in enterobacteriaceae bacteremia. inpatients with bacteremia caused by esbl-producing escherichia coli, klebsiella spp., or proteus spp. (cases) were compared with patients with bacteremia caused by non-esbl producers (controls). outcomes included mortality, mortality due to infection, length of stay (los), delay in appropriate therapy (dat), discharge to a chronic care facility, and hospital cost. ninety-nine cases and 99 ...200616569837
in vitro activities of three carbapenems against recent bacterial isolates from severely ill patients at swedish hospitals.to study the in vitro activity of imipenem, meropenem and ertapenem against common pathogens isolated from patients in intensive care, haematology and dialysis/nephrology units at 7 swedish university hospitals, a total of 788 isolates were collected during 2002-2003. the distribution of the isolates was as follows: escherichia coli (n = 140), klebsiella spp. (n = 132), proteus spp. (n = 97), enterobacter spp. (n = 113), pseudomonas aeruginosa (n = 126), acinetobacter spp. (n = 53) and enterococ ...200617008228
urinary bactericidal activity of oral antibiotics against common urinary tract pathogens in an ex vivo model.in this investigation, the urine samples obtained in a single oral-dose pharmacokinetic study were examined for their bactericidal activity against a range of relevant urinary tract pathogens.200617008781
etiologic agents of cervicovaginitis in turkish women.to investigate the distribution of microbiologic agents causing cervicovaginitis.200617013472
the mechanism of plasmid curing in bacteria.bacterial plasmids have a major impact on metabolic function. lactose fermentation of e. coli or hemolysin b transporter expressed by the plasmids that carry these respective genes could be readily obviated by heterocyclic compounds that readily bind to plasmid dna. these compounds could also reverse the resistance to antibiotics of e. coli, enterobacter, proteus, staphylococcus and yersinia strains by eliminating plasmids. however, the frequency and extent of this effect was significantly less ...200616842214
[antimicrobial susceptibility of proteus mirabilis urinary tract isolates from 1999 to 2005 at nîmes university hospital].to analyse antimicrobial resistance trends of proteus mirabilis urinary tract isolates from 1999 to 2005 at the nîmes university hospital.200617030456
cysteine based novel noncompetitive inhibitors of urease(s)--distinctive inhibition susceptibility of microbial and plant ureases.based on the catalysis mechanism of urease, a homologous series of 10 cysteine derivatives (cysds) was designed and synthesized, and their inhibitory activities were evaluated for microbial ureases (bacillus pasteurii, bpu, and proteus mirabilis, pmu) and for a plant urease [jack bean (cavavalia ensiformis), jbu]. as already described, thiol-compounds might inhibit urease activity by chelating the nickel atoms involved in the catalysis process. in contrast to cysteine, which has been reported to ...200616859909
proteus syndrome with agenesis of the rectus abdominis. 200617034561
proteus syndrome with huge tonsillar mass causing dysphagia: a rare case.proteus syndrome is a rare and highly variable hamartomatous syndrome that can affect multiple organ systems. it is characterized by hyperplasia of connective tissue, vascular malformations, epidermal naevi and hyperostosis. most of the cases present to plastic and orthopaedic surgeons. otolaryngologic presentations are minimal. we report such a rare case, which presented with a huge unilateral tonsillar mass causing dysphagia.200616556346
surgical site infections in day surgery settings.because major complications are rare in day surgery, minor morbidities such as surgical site infections, which become evident only after patient discharge, have a major impact on the perceived quality of care. we performed an audit of such infections over a oneyear period.200616895493
new trends in prosthesis infection in cardiovascular surgery.adult cardiovascular surgery entails, in many cases, the use of some kind of prosthesis. among the potential complications, prosthetic device infection is one of the most devastating in incidence, as well as in prognosis and damage to surrounding tissues.200616895504
a case of proteus syndrome (elephant man). 200616898038
[microorganisms isolated from middle ear cultures and their antibacterial susceptibility in patients with chronic suppurative otitis media].this study sought to determine causative microorganisms of chronic suppurative otitis media and their antibacterial susceptibility.200616905907
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (2004). i. susceptibility distribution].the bacterial strains isolated from 490 patients diagnosed as having urinary tract infections (utis) in 14 institutions in japan were collected between august 2004 and july 2005. the susceptibilities of them to many kinds of antimicrobial agents were measured. of them, 577 strains were estimated as causative bacteria and used for the measurement. the strains consisted of 156 gram-positive bacterial strains (27.0%) and 421 gram-negative bacterial strains (73.0%). against staphylococcus aureus, ar ...200616913404
how long do nosocomial pathogens persist on inanimate surfaces? a systematic review.inanimate surfaces have often been described as the source for outbreaks of nosocomial infections. the aim of this review is to summarize data on the persistence of different nosocomial pathogens on inanimate surfaces.200616914034
pcr for the identification and differentiation of histomonas meleagridis, tetratrichomonas gallinarum and blastocystis spp.in the present investigation pcr assays were developed for the rapid detection and differentiation of two poultry flagellates: histomonas meleagridis and tetratrichomonas gallinarum as well as the protozoan microorganism: blastocystis spp. the nucleotide sequences of the small subunit ribosomal rnas were used for primer construction obtaining fragments which vary in size for each microorganism. the established pcrs were able to detect dna obtained from one microorganism of t. gallinarum and blas ...200616920265
case report: acute unintentional carbachol intoxication.intoxications with carbachol, a muscarinic cholinergic receptor agonist are rare. we report an interesting case investigating a (near) fatal poisoning.200616740173
fishmeal extract bile salt lactose agar--a differential medium for enteric bacteria.fishmeal extract bile salt lactose agar (febla), a new differential medium for enteric bacteria was developed and evaluated for its ability to grow and differentiate lactose fermenters (lf) from non-lactose fermenters (nlf) in comparison with macconkeys agar. performance of febla was at par with the latter. on febla medium, the contrast between lf and nlf colonies was pronounced and klebsiella pneumoniae produced more mucoid colonies than on macconkeys agar (hi media). unlike macconkeys agar, a ...200616924840
emerging resistance to carbapenems in a tertiary care hospital in north india.carbapenems are beta-lactam antibiotics, presently considered as most potent agents for treating multi-drug resistant gram-negative bacilli infections. in india carbapenems available for use are meropenem and imipenem, introduced recently. resistance to these has been reported in a few bacteria especially pseudomonas spp. we therefore retrospectively evaluated the antibiotic susceptibility pattern to these agents amongst various clinical isolates in a tertiary care hospital in north india.200616926463
extended-spectrum beta-lactamases in taiwan: epidemiology, detection, treatment and infection control.extended-spectrum beta-lactamases (esbls) efficiently hydrolyze extended-spectrum beta-lactams such as cefotaxime, ceftriaxone, ceftazidime, and aztreonam. esbls are most often plasmid-mediated. in taiwan, the prevalence of esbls in bacteria has risen, ranging from 8.5 to 29.8% in klebsiella pneumoniae and 1.5 to 16.7% in escherichia coli isolates. the most prevalent types of esbls are shv-5, shv-12, ctx-m-3, and ctx-m-14 in isolates of k. pneumoniae and e. coli, with differences between institu ...200616926972
1h nmr spectroscopy in the diagnosis of klebsiella pneumoniae-induced urinary tract infection.the (1)h nmr spectroscopic method is suggested and its utility is demonstrated for the diagnosis of klebsiella pneumoniae (k. pneumoniae) in urinary tract infection (uti). k. pneumoniae have the specific property of metabolizing glycerol to 1,3-propanediol (1,3-pd), acetate, ethanol and succinate. the quantity of 1,3-pd produced correlates well with the viable bacterial count. other common bacteria causing uti (except for citrobacter frundii), such as escherichia coli (e. coli), pseudomonas aeru ...200616927393
comparison of susceptibility to antimicrobials of bacterial isolates from companion animals in a veterinary diagnostic laboratory in canada between 2 time points 10 years apart.the susceptibility to antimicrobials of bacterial species most frequently isolated from companion animals in a veterinary teaching diagnostic laboratory was evaluated retrospectively. a significant decrease between 1990-1992 and 2002-2003 was noted in the susceptibility of dog isolates to the following antimicrobials: escherichia coli to cephalothin (86% to 61%, p < 0.001); e. coli to ampicillin (85% to 67%, p < 0.001); proteus spp. to ampicillin (92% to 71%, p < 0.01); coagulase-positive staphy ...200616933555
assessment of pathogen frequency and resistance patterns among pediatric patient isolates: report from the 2004 sentry antimicrobial surveillance program on 3 continents.selecting empiric or directed therapy for pathogens isolated from pediatric patients can be problematic. many antimicrobial agents are not indicated for use in pediatric patients, and regional variations of resistance mechanisms have been reported. the purpose of this study was to analyze antimicrobial resistance patterns and pathogen occurrence rates in pediatric-aged patient infections on 3 continents using data from the sentry antimicrobial surveillance program. a total of 3537 clinical isola ...200616938419
effective prevention of microbial biofilm formation on medical devices by low-energy surface acoustic waves.low-energy surface acoustic waves generated from electrically activated piezo elements are shown to effectively prevent microbial biofilm formation on indwelling medical devices. the development of biofilms by four different bacteria and candida species is prevented when such elastic waves with amplitudes in the nanometer range are applied. acoustic-wave-activated foley catheters have all their surfaces vibrating with longitudinal and transversal dispersion vectors homogeneously surrounding the ...200616940055
mode of transposition and expression of 16s rrna methyltransferase gene rmtc accompanied by isecp1.a newly identified 16s rrna methyltransferase gene, rmtc, was accompanied by an isecp1 element at its 5' end. this isecp1 element, which contained a transposase gene, tnpa, provided a promoter activity for expression of the adjacent rmtc; and this structure enabled the rmtc gene to be transposed onto another plasmid in escherichia coli.200616940134
relevance of weil-felix test in diagnosis of scrub typhus in india.to study the relationship of weil-felix test and microimmunofluorescence test.200616941792
antibiotic susceptibility tests directly on urine samples using uro-quick, a rapid automated system.during the period june 2003-march 2004, 12,579 urine samples were examined employing the uro-quick system. positive samples (1,948) were subsequently gram-stained and processed by standard procedures for microorganism identification and antibiotic susceptibility determination by the disk diffusion method. results of this latter test were compared with those obtained employing the new rapid uro-quick method. antibiotics were introduced in vials containing 2 ml of mueller-hinton broth, then 0.5 ml ...200616572888
rheumatoid arthritis is an autoimmune disease triggered by proteus urinary tract infection.rheumatoid arthritis (ra) is a chronic and disabling polyarthritic disease, which affects mainly women in middle and old age. extensive evidence based on the results of various microbial, immunological and molecular studies from different parts of the world, shows that a strong link exists between proteus mirabilis microbes and ra. we propose that sub-clinical proteus urinary tract infections are the main triggering factors and that the presence of molecular mimicry and cross-reactivity between ...200616603443
the dps gene of symbiotic "candidatus legionella jeonii" in amoeba proteus responds to hydrogen peroxide and phagocytosis.to survive in host cells, intracellular pathogens or symbiotic bacteria require protective mechanisms to overcome the oxidative stress generated by phagocytic activities of the host. by genomic library tagging, we cloned a dps (stands for dna-binding protein from starved cells) gene of the symbiotic "candidatus legionella jeonii" organism (called the x bacterium) (dps(x)) that grows in amoeba proteus. the gene encodes a 17-kda protein (pi 5.19) with 91% homology to dps and dna-binding ferritin-l ...200616950918
ciprofloxacin crystal nephropathy--a 'new' cause of acute renal failure. 200616611679
analysis on antimicrobial resistance of clinical bacteria isolated from county hospitals and a teaching hospital.the distinction of antimicrobial resistance of clinical bacteria isolated from county hospitals and a teaching hospital was investigated. disc diffusion test was used to study the antimicrobial resistance of isolates collected from county hospitals and a teaching hospital. the data was analyzed by whonet5 and spss statistic software. a total of 655 strains and 1682 strains were collected from county hospitals and a teaching hospital, respectively, in the year of 2003. the top ten pathogens were ...200616961303
occurrence of qnra-positive clinical isolates in french teaching hospitals during 2002-2005.bacteria harbouring the novel qnra plasmid-mediated mechanism of quinolone resistance have been described in different countries, but the frequency of their occurrence has not been investigated. in total, 1,468 clinical isolates of enterobacteriaceae with quinolone resistance or extended-spectrum beta-lactamase (esbl) phenotypes were collected from eight teaching hospitals in france during 2002-2005 and screened for qnra. overall, 28 isolates (22 enterobacter cloacae, three klebsiella pneumoniae ...200616961639
comparison of fecal microflora in children with atopic eczema/dermatitis syndrome according to ige sensitization to food.atopic eczema/dermatitis syndrome (aeds) commonly often arises during early infancy. in several intervention studies a beneficial influence on aeds course of certain intestinal bacteria, administered as 'probiotics', has been described. to evaluate the possible role of the natural intestinal microflora in children with allergic eczema/dermatitis syndrome regarding immediate type hypersensitivity to food allergens, children with food allergy (aaeds, n = 68) have been compared with children withou ...200616618364
synthesis, structure and biological activity of cobalt(ii) and copper(ii) complexes of valine-derived schiff bases.we have synthesized two cobalt(ii) 2 and copper(ii) 3 complexes of valine-derived schiff bases. the obtained complexes were characterized by elemental analysis, ft-ir and x-ray diffraction. biological studies of complexes 2 and 3 had been carried out in vitro for antimicrobial activity against gram-positive, gram-negative bacteria and human pathogenic fungi. compound 3 was proven to be a broad spectrum agent, showed a significant inhibition of the growth of gram-positive bacteria (staphylococcus ...200616965817
ft-ir microspectroscopy for early identification of some clinically relevant pathogens.to investigate the potentials and limitations of fourier transform-infrared (ft-ir) microspectroscopy as a tool to identify, at the level of microcolonies, pathogenic bacteria frequently isolated in the clinical environment.200616968290
a novel gene involved in regulating the flagellar gene cascade in proteus mirabilis.in this study, we identified a transposon insertion in a novel gene, designated disa, that restored swarming motility to a putrescine-deficient spea mutant of proteus mirabilis. a null allele in disa also increased swarming in a wild-type background. the disa gene product was homologous to amino acid decarboxylases, and its role in regulating swarming was investigated by examining the expression of genes in the flagellar cascade. in a disa mutant background, we observed a 1.4-fold increase in th ...200616980463
lipid-specific membrane activity of human beta-defensin-3.defensins represent a major component of innate host defense against bacteria, fungi, and enveloped viruses. one potent defensin found, e.g., in epithelia, is the polycationic human beta-defensin-3 (hbd3). we investigated the role of the lipid matrix composition, and in particular the presence of negatively charged lipopolysaccharides (lps) from sensitive (escherichia coli, salmonella enterica serovar minnesota) or resistant (proteus mirabilis) gram-negative bacteria or of the zwitterionic phosp ...200616634647
[sensitivity and resistance of aerobic bacteria isolated from patients with periodontitis towards antibiotics and bacteriophages (comparative analysis)].in order to examine sensitivity and resistance of isolated aerobic bacteria from periodontitis materials towards antibiotics and bacteriophages, there has been studied exudations taken from 737 patients' periodontic pockets or the tissue taken from curettage. according to the rate of identified microorganisms, they have been arranged as follows: s. epidermidis 39,34+/-1,56%; s. pyogenes 18,84+/-1,25%; m. catarrhalis 17,09+/-1,2%; s. aureus 10,71+/-0,99%; e.coli-5,66+/-0,74%; diphtheroids in 1,13 ...200616636376
rapid onset of ulcerative typhlocolitis in b6.129p2-il10tm1cgn (il-10-/-) mice infected with helicobacter trogontum is associated with decreased colonization by altered schaedler's flora.infection with helicobacter trogontum, a urease-positive helicobacter isolated from subclinically infected rats, was evaluated in b6.129p2-il10(tm1cgn) (interleukin-10(-/-) [il-10(-/-)]) and c57bl/6 (b6) mice. in a first experiment, il-10(-/-) mice naturally infected with helicobacter rodentium had subclinical typhlocolitis but developed severe diarrhea and loss of body condition with erosive to ulcerative typhlocolitis within 1 to 3 weeks of experimental infection with h. trogontum. a second ex ...200616982822
multiple bacterial species reside in chronic wounds: a longitudinal study.the aim of the study was to investigate the bacterial profile of chronic venous leg ulcers and the importance of the profile to ulcer development. patients with persisting venous leg ulcers were included and followed for 8 weeks. every second week, ulcer samples were collected and the bacterial species present were identified. more than one bacterial species were detected in all the ulcers. the most common bacteria found were staphylococcus aureus (found in 93.5% of the ulcers), enterococcus fae ...200616984578
[development of a gold-immunochromatography test for rapid detecting e. coli o157].to develop a method for rapid detecting escherichia coli (e. coli) o157 on site.200616986519
antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents.the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ...200616989308
diabetic foot ulcers in cameroon: can microflora prevalence inform probabilistic antibiotic treatment?to determine the clinical features, regularly associated microorganisms and their susceptibility to antibiotics, and the clinical outcomes of foot ulcers in patients with diabetes at the yaoundé central hospital, cameroon.200617001946
phytochemical and antimicrobial study of an antidiabetic plant: scoparia dulcis l.the antimicrobial and antifungal effects of different concentrations of chloroform/methanol fractions of scoparia dulcis were investigated. the isolated fractions were tested against different bacteria like salmonella typhii, staphylococcus aureus, escherichia coli, bacillus subtilis, pseudomonas aeruginosa, and proteus vulgaris and fungal strains such as alternaria macrospora, candida albicans, aspergillus niger, and fusarium oxysporum. the isolated fractions exhibited significant antimicrobial ...200617004904
inhibition of swarming and virulence factor expression in proteus mirabilis by resveratrol.resveratrol (3,5,4-trihydroxy-trans-stilbene) is a phytoalexin compound with anti-inflammatory and antioxidant activities. the effect of resveratrol on swarming and virulence factor expression of proteus mirabilis, an important pathogen infecting the urinary tract, was determined on swarming agar plates with and without the compound. bacteria harvested at different times were assayed for cell length and the production of flagella, haemolysin and urease. resveratrol inhibited p. mirabilis swarmin ...200617005777
nutritive value, antioxidant and antimicrobial properties of struchium sparganophora leaves.struchium sparganophora leaf is a green leafy vegetable popularly used in soup preparations in processed and unprocessed forms in nigeria. fresh leaves of s. sparganophora were soaked overnight to remove its bitterness, before determination of the nutrient and antinutrient content, while sun-dried s. sparganophora leaves were extracted with rectified spirit. the antioxidant and antimicrobial properties of the extracts were subsequently determined. the result of the study revealed that the fresh ...200616822216
pharmacokinetic and pharmacodynamic aspects of antimicrobial agents for the treatment of uncomplicated urinary tract infections.uncomplicated urinary tract infections (uti) are treated with beta-lactams, co-trimoxazole, quinolones and fosfomycin tromethamine. due to increasing resistance of causative pathogens, antibiotics should be used by considering their pharmacodynamic and pharmacokinetic characteristics. beta-lactams have time-dependent activity and should not be used once-daily. co-trimoxazole should be restricted due to increasing chemoresistance. fluoroquinolones play a primary role in the treatment of serious a ...200616829051
hospital acquired urinary tract infections in urology departments: pathogens, susceptibility and use of antibiotics. data from the pep and peap-studies.data from two internet-based studies on nauti in hospitalized urological patients are presented together: the pan european prevalence (pep) study, which was a 1-day prevalence study in november 2003; and the pan euro-asian prevalence (peap) study, which was carried out in november 2004. overall, 93 and 101 hospitals from the two studies, respectively, completed the hospital questionnaires and provided patient information for the present study. nauti was diagnosed according to the centres for dis ...200616829052
urinary tract infection caused by fluoroquinolone- and cephem-resistant enterobacteriaceae.in 2001, fluoroquinolone-resistant escherichia coli isolates emerged in japan in patients with uncomplicated urinary tract infection (uti), and accounted for ca. 8% of isolates. this is a worldwide occurrence as reported by the eco.sens study. the number of fluoroquinolone- and cephem-resistant enterobacteriaceae isolates from patients with complicated uti is increasing. most cephem-resistant isolates of e. coli, klebsiella pneumoniae and proteus mirabilis produce extended-spectrum beta-lactamas ...200616829056
[wound care management of ulceration in a palliative care patient].the wound care management of a palliative care patient with sinusitis pinoidalis is documented. the treatment is validated by the expert's opinion. there is only little evidence-based treatment of such a lesion. in our experience, photo documentation is helpful in decision-making during the treatment process. in addition, healing of larger defects seems to improve upon frequent and extensive liquid cleansing.200616830251
structure of the glycerol phosphate-containing o-polysaccharides and serological studies of the lipopolysaccharides of proteus mirabilis ccug 10704 (oe) and proteus vulgaris tg 103 classified into a new proteus serogroup, o54.o-polysaccharides were obtained from the lipopolysaccharides of proteus mirabilis ccug 10704 (oe) and proteus vulgaris tg 103 and studied by chemical analyses and one- and two-dimensional (1)h and (13)c nuclear magnetic resonance spectroscopy, including rotating-frame nuclear overhauser effect spectroscopy, h-detected (1)h,(13)c heteronuclear single-quantum spectroscopy and (1)h,(31)p heteronuclear multiple-quantum spectroscopy experiments. the proteus mirabilis oe polysaccharide was found to ha ...200616831214
[a puzzle of the physician and the patient]. 200616835961
[clinical effect of continuous infusion of meropenem on bacterial pneumonia in the elderly].we studied the clinical effect of continuous infusion over 24 hours of meropenem (mepm) on bacterial pneumonia in the elderly (over 65). the subjects were 26 patients (community-acquired pneumonia: moderate, n = 9; severe, n= 4; hospital-acquired pneumonia: group iii, n = 13) whose performance status was 3 or 4. mepm 1.0g/day was infused continuously for 7-14 days, and its clinical efficacy, bacteriological efficacy, and side effects were examined prospectively. it was effective in 23 of the 26 ...200616841713
factors affecting crystal precipitation from urine in individuals with long-term urinary catheters colonized with urease-positive bacterial species.weekly urinalysis was conducted for 12 weeks on a group of 21 long-term catheter users with confirmed catheter encrustation and urinary tract colonization with urease-positive bacteria, in order to explore the cause of considerable variation in the severity of encrustation between sufferers. the rapidity of catheter blockage correlated significantly with the ph above which crystals precipitated from urine (the nucleation ph) but not the ph of the voided urine itself. linear regression showed the ...200616453146
doppler flow imaging of cytoplasmic streaming using spectral domain phase microscopy.spectral domain phase microscopy (sdpm) is a function extension of spectral domain optical coherence tomography. sdpm achieves exquisite levels of phase stability by employing common-path interferometry. we discuss the theory and limitations of doppler flow imaging using sdpm, demonstrate monitoring the thermal contraction of a glass sample with nanometer per second velocity sensitivity, and apply this technique to measurement of cytoplasmic streaming in an amoeba proteus pseudopod. we observe r ...200616674204
epidermal naevus in proteus syndrome showing loss of heterozygosity for an inherited pten mutation.a 3-year-old boy with proteus syndrome has a novel germline p.y68d mutation of the pten gene inherited from his mother who has cowden syndrome. in addition, dna extracted from curettings of his widespread epidermal naevus shows loss of heterozygosity for this mutation. to our knowledge, this has not been described before.200616704655
structures and serology of the o-antigens of proteus strains classified into serogroup o17 and former serogroup o35.bacteria of the genus proteus are facultative pathogens which commonly cause urinary tract infections. based on the serological specificity of the o-chain polysaccharide of the lipopolysaccharide (o-polysaccharide, o-antigen), strains of p. mirabilis and p. vulgaris have been classified into 60 serogroups. studies on the chemical structure and serological specificity of the o-antigens aim at the elucidation of the molecular basis and improvement of the serological classification of these bacteri ...200616868723
antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents.the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ...200616869486
the potential use of antibacterial peptide antibody indices in the diagnosis of rheumatoid arthritis and ankylosing spondylitis.both rheumatoid arthritis (ra) and ankylosing spondylitis (as) are potentially disabling arthritic disorders for which as yet no highly sensitive and reliable diagnostic laboratory markers are available.200616484874
systemic absorption of gentamicin nasal irrigations.to determine if gentamicin nasal irrigation is systemically absorbed, and to identify any ototoxic side effects related to its use.200616871924
hemihyperplasia syndromes.hemihyperplasia is a heterogenous group of disorders characterized by asymmetric limb growth. there is considerable confusion regarding their classification and ascertainment into various syndromes. we tried to look into the various aspects of hemihyperplasia syndromes.200616877856
the first detection of ctx-m-14 extended-spectrum beta-lactamase among diverse beta-lactamase-producing proteus mirabilis clinical isolates.ctx-m-14- and -2-like extended-spectrum beta-lactamases were detected in 5 and 1 proteus mirabilis isolates, respectively, among 92 non-duplicate strains tested from december 2002 to september 2003. this is the first report of the ctx-m-14 enzyme in p. mirabilis to the best of our knowledge and suggests that the ctx-m-type enzyme is gradually spreading to this species. bla(tem), bla(shv-1), and/or bla(oxa-10) alleles were also detected in 35 ampicillin-resistant isolates tested.200616427241
emergence of proteus mirabilis carrying the bla metallo-beta-lactamase gene.seven genetically related proteus mirabilis clinical isolates from a hospital in thessaloniki, greece, exhibited decreased susceptibility to imipenem and carried a bla(vim-1) metallo-beta-lactamase gene. pcr mapping revealed that bla(vim-1) was part of a class 1 integron that was probably located in the chromosome and also included the aaca7, dhfr and aada genes. this is the first description of the bla(vim-1) metallo-beta-lactamase gene in p. mirabilis.200616774572
improving the accuracy of protein secondary structure prediction using structural alignment.the accuracy of protein secondary structure prediction has steadily improved over the past 30 years. now many secondary structure prediction methods routinely achieve an accuracy (q3) of about 75%. we believe this accuracy could be further improved by including structure (as opposed to sequence) database comparisons as part of the prediction process. indeed, given the large size of the protein data bank (>35,000 sequences), the probability of a newly identified sequence having a structural homol ...200616774686
biofilm formation in an ice cream plant.the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ...200616779628
[extended-spectrum-beta-lactamase-producing proteus mirabilis: laboratory-based surveillance in cooperation with 12 clinical laboratories in the kinki region of japan].we studied 247 strains of proteus mirabilis collected during the 6 months from november 2003 to april 2004 from 12 clinical laboratories in the kinki region of japan for the production of extended-spectrum beta-lactamase (esbl). eighteen strains (7.3%) showed mics for cefpodoxime of > or = 2 microg/ml and 13 strains (5.2%) were positive for the double-disk synergy test. susceptibility depended on genotype. mics for cefepime, cefozopran, and cefpirome were high (> or = 8 microg/ml), and that for ...200616780129
synthesis and in vitro antimicrobial studies of medicinally important novel n-alkyl and n-sulfonyl derivatives of 1-[bis(4-fluorophenyl)-methyl]piperazine.a series of novel substituted 1-[bis(4-fluorophenyl)-methyl]piperazine derivatives (4a-g) and (5h-m) have been synthesized. the synthesized compounds were characterized by ir and 1h nmr. all the synthesized compounds were evaluated in vitro for their efficacy as antimicrobial agents against representative strains of gram-positive (staphylococcus aureus atcc 25953, streptococcus pneumoniae atcc 49619, bacillus cereus 11778, and bacillus subtilis 6051) and gram-negative bacteria (escherichia coli ...200616784863
surgical treatment of an unusual patient with proteus syndrome. 200616432429
tigecycline: a new glycylcycline antimicrobial agent.the pharmacology, spectrum of activity, pharmacokinetics, clinical efficacy, adverse events, dosage and administration, drug interactions, and place in therapy of tigecycline are reviewed.200616790575
structure of escherichia coli tryptophanase.pyridoxal 5'-phosphate (plp) dependent tryptophanase has been isolated from escherichia coli and its crystal structure has been determined. the structure shares the same fold with and has similar quaternary structure to proteus vulgaris tryptophanase and tyrosine-phenol lyase, but is found in a closed conformation when compared with these two enzymes. the tryptophanase structure, solved in its apo form, does not have covalent plp bound in the active site, but two sulfate ions. the sulfate ions o ...200616790938
capillary isoelectric focusing of microorganisms in the ph range 2-5 in a dynamically modified fs capillary with uv detection.the isoelectric points of many microbial cells lie within the ph range spanning from 1.5 to 4.5. in this work, we suggest a cief method for the separation of cells according to their isoelectric points in the ph range of 2-5. it includes the segmental injection of the sample pulse composed of the segment of the selected simple ampholytes, the segment of the bioanalytes and the segment of carrier ampholytes into fused silica capillaries dynamically modified by poly(ethylene glycole). this polymer ...200616791563
a simple phenotypic method for differentiation between acquired and chromosomal ampc beta-lactamases in escherichia coli.screening methods for the detection of plasmid-mediated ampc beta-lactamases are technically demanding. the purpose of this study was to assess screening methods for the detection of these enzymes in clinical isolates of escherichia coli, klebsiella pneumoniae and proteus mirabilis.200616792938
gram-negative bacteria from patients seeking medical advice in stockholm after the tsunami catastrophe.microbiological cultures from 229 patients seeking medical advice in stockholm after the tsunami catastrophe of december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative bacilli were the most common findings from wound cultures. common human pathogens such as escherichia coli, proteus species, klebsiella spp., and pseudomonas aeruginosa were isolated. more rare species of gram-negative bacilli, e.g. myroides odoratus, sp ...200616798691
proteus mirabilis spondylodiscitis complicating a urinary tract infection.infectious spondylodiscitis results from local or haematogenous infection by microorganisms of intervertebral disc spaces, vertebral bodies and surrounding structures. haematogenous invasion may follow urosepsis. we report on a case of septic spondylodiscitis following urosepsis with proteus mirabilis, a frequently isolated microorganism in urinary tract infections but rarely in spondylodiscitis.200616798719
the ability of flagellum-specific proteus vulgaris bacteriophage pv22 to interact with campylobacter jejuni flagella in culture.there has been a recent resurgent interest in bacteriophage biology. research was initiated to examine campylobacter jejuni-specific bacteriophage in the russian federation to develop alternative control measures for this pathogen.200616803630
proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice.proteus mirabilis has been described as an aetiological agent in a wide range of infections, playing an important role in urinary tract infections (utis). in this study, a collection of p. mirabilis isolates obtained from clinical and non-clinical sources was analysed in order to determine a possible correlation between origin, virulence factors and in vivo infectivity. isolates were characterized in vitro, assessing several virulence properties that had been previously associated with p. mirabi ...200616804188
mapping bacterial surface population physiology in real-time: infrared spectroscopy of proteus mirabilis swarm colonies.we mapped the space-time distribution of stationary and swarmer cells within a growing proteus mirabilis colony by infrared (ir) microspectroscopy. colony mapping was performed at different positions between the inoculum and the periphery with a discrete microscope-mounted ir sensor, while continuous monitoring at a fixed location over time used an optical fiber based ir-attenuated total reflection (atr) sensor, or "optrode." phenotypes within a single p. mirabilis population relied on identific ...200616808858
proteus syndrome with multiple genitourinary abnormalities.proteus syndrome is a rare hamartomatous disorder comprising a broad spectrum of congenital malformations and overgrowth of multiple tissues. some rare urogenital malformations have been reported before. this case is unique for the presence of multiple genitourinary anomalies including retroperitoneal cystic mass, intra-abdominal testicle with hematoma and ureterovesical stricture apart from common clinic findings of proteus syndrome.200616401930
the anti-microbial activity of electrolysed oxidizing water against microorganisms relevant in veterinary medicine.standards of the german association of veterinary medicine (dvg) for the evaluation of chemical disinfectants were used to assess the anti-microbial efficacy of electrolysed oxidizing water (eow). enterococcus faecium, mycobacterium avium subspecies avium, proteus mirabilis, pseudomonas aeruginosa, staphylococcus aureus and candida albicans were exposed to anode eow (ph, 3.0+/-0.1; oxidation-reduction potential (orp), +1100+/-50 mv; free chlorine, 400+/-20 mg/l cl2) and combined eow (7:3 anode:c ...200616629725
observations on the adherence of proteus mirabilis onto polymer surfaces.infection of the catheterized urinary tract with proteus mirabilis causes blockage of the catheter by crystalline bacterial biofilms. the aim of this work is to identify a surface-coating for catheters that is not vulnerable to colonization by pr. mirabilis.200616630003
isolation of salmonella bacteriophages from swine effluent lagoons.bacteriophages (phages) associated with salmonella were collected from nine swine manure lagoons in mississippi. phages were isolated by an enrichment protocol or directly from effluent. for enrichment, chloroform-treated samples were filtered (0.22 mum) and selectively enriched by adding a cocktail of salmonella strains in trypticase soy broth. after overnight incubation at 35 degrees c, chloroform was added and samples stored at 5 degrees c. enriched samples were tested by double agar layer (d ...200616455853
pathogenic bacteria carried by companion animals and their susceptibility to antibacterial agents.results of the investigation showed that there was a difference in the bacteria isolated from dogs, cats and their living environment. the number and species isolated from the hair and front paw samples from dogs kept outdoors and from cats were greater and more varied than those from the samples from dogs kept indoors. staphylococcus, micrococcus and bacillus were frequently detected from skin surfaces. on the other hand, escherichia, pseudomonas, proteus and others were detected on each sampli ...200616637433
comparative evaluation of bact/alert 3d and bactec systems for the recovery of pathogens causing bloodstream infections.to compare bact/alert (bta) and bactec 9240 (bac), two continuously monitoring automated blood culture systems, for the recovery of bloodstream pathogens and standard media available for these systems.200616651840
trends in production of extended-spectrum beta-lactamases among enterobacteria of medical interest: report of the second italian nationwide survey.results of a 2003 survey carried out in italy to evaluate the prevalence of extended-spectrum beta-lactamase (esbl)-producing enterobacteria are presented. eleven italian microbiology laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). esbl screening was performed by mic data analysis. confirmation was obtained using the double-disk synergy test and the combination disk test based on clsi methodology. esbl determinants were investigated by c ...200616672390
functional grouping based on signatures in protein termini.the two ends of each protein are known as the amino (n-) and carboxyl (c-) termini. short signatures in a protein's termini often carry vital cellular function. no systematic research has been conducted to address the importance of short signatures (3 to 10 amino acids) in protein termini at the proteomic level. specifically, it is unknown whether such signatures are evolutionarily conserved, and if so, whether this conservation confers shared biological functions. current signature detection me ...200616475191
performance in detection and reporting beta-lactam resistance phenotypes in enterobacteriaceae: a nationwide proficiency study in italian laboratories.we evaluated the ability of 60 italian clinical microbiology laboratories in detecting and reporting beta-lactam resistance phenotypes in enterobacteriaceae. laboratories received 5 well-characterized isolates producing extended-spectrum beta-lactamases (esbls), 2 hyperproducers of chromosomal enzymes, and 3 quality control strains. the performances in antimicrobial susceptibility testing (ast) were different depending on the species and type of esbl produced. high rates of very major errors (up ...200616678993
[patterns of susceptibility to antibiotics of enterobacteriaceae causing intra-abdominal infection in spain: smart 2003 study outcomes].smart (study for monitoring antimicrobial resistance trends) is an ongoing global antimicrobial surveillance program focused on clinical isolates from intra-abdominal infections. the objective of this subanalysis was to assess antimicrobial susceptibility patterns among entero-bacteriaceae recovered at 13 participating spanish sites during 2003. antimicrobial susceptibility testing was performed using broth microdilution techniques according to the clsi (formerly nccls) guidelines for mic testin ...200616688292
antibacterial and antioxidant activities in sideritis italica (miller) greuter et burdet essential oils.sideritis italica (miller) greuter et burdet is a widespread lamiacea in the mediterranean region used in traditional medicine. essential oils were antibacterial against nine atcc and as many clinically isolated gram-positive and gram-negative bacterial strains. antibacterial activity was also found against helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. the antibacterial activity of the oils was expressed as mics (minimum inhibitory concentrations) and mbc ...200616690235
otogenic cerebellar abscess: an unusual occurrence. 200616696351
triclosan loaded ureteral stents decrease proteus mirabilis 296 infection in a rabbit urinary tract infection model.infection and encrustation remain major limitations of ureteral stent use and to our knowledge no device has completely overcome these obstacles to date. triclosan is a biocide currently used in a plethora of consumer and medical products that has recently been loaded into a ureteral stent. using a rabbit model of uti we examined the effects of triclosan impregnated stent segments on the growth and survival of proteus mirabilis, a uropathogen commonly associated with device related uti and encru ...200616697868
structure of the o-polysaccharide of proteus mirabilis ccug 10705 (of) containing an amide of d-galacturonic acid with l-alanine.the structure of the o-polysaccharide of proteus mirabilis ccug 10705 (of) was determined by chemical analyses along with one- and two-dimensional (1)h and (13)c nmr spectroscopy. the polysaccharide was found to contain an amide of d-galacturonic acid with l-alanine and based on the uniqueness of the o-polysaccharide structure and serological data, it was suggested to classify p. mirabilis of into a new separate proteus serogroup, o74. a weak cross-reactivity of p. mirabilis of and p. mirabilis ...200616697996
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