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antimicrobial susceptibility of klebsiella spp. and proteus spp. from various organ systems of horses, dogs and cats as determined in the bft-germvet monitoring program 2004-2006.a total of 120 isolates of klebsiella spp. and proteus spp. collected from horses and small animals (dogs and cats) were screened for their susceptibility to 24 different antimicrobial agents. klebsiella spp. were included from infections of the genital tract (gt) of horses (36 isolates) and the urinary/genital tract (ugt) from dogs and cats (17 isolates), while proteus spp. were from small animal (dogs and cats) infections of the ugt (37 strains) and the skin (incl. ear/mouth) (30 isolates). in ...200717939455
proteus syndrome in the aetiology of carpal tunnel syndrome. 200717939492
anti-amebic antibody activity in patients, determined with antigens prepared from virulent parasites (indirect hemagglutination assay and enzyme-linked immunosorbent assay).the serology of amebiasis is affected by low sensitivity and specificity.200717939629
effect of natural preservatives on the growth of histamine producing bacteria.present study deals with the hampering of the growth of histamine producing bacteria (hpb), by using nacl and spices which are easily available and cheaper cost wise. for this experiment, four strains of hpb viz. vibrio parahaemolyticus, bacillus cereus, pseudomonas aeruginosa and proteus mirabilis were tested against 1 to 10% concentrations of nacl and 1 to 5% concentrations of natural preservatives (turmeric, ginger and garlic) in a basal medium. hpb showed different growth rates at different ...200717915763
cowden syndrome.cowden syndrome (cs), due to germline mutations of the pten tumor-suppressor gene, is an often overlooked cancer predisposition syndrome associated with an increased risk of breast, thyroid, and endometrial cancers, as well as benign manifestations. germline pten mutations also are associated with syndromes that have not been historically connected to an increase in risk for malignancy. these disorders include bannayan-riley-ruvalcaba syndrome (brrs), proteus syndrome (ps), and proteus-like synd ...200717920899
bacterial contaminations of informally marketed raw milk in ghana.summary200717925843
brain abscess in infants and children: a retrospective study of 107 patients in northeast thailand.to review the clinical manifestation, predisposing factor location of abscess, causative organism, treatment, and outcome of brain abscess.200717926990
[phosphatase activity in amoeba proteus at ph 9.0].in the free-living amoeba amoeba proteus (strain b), after paag disk-electrophoresis of the homogenate supernatant, at using 1-naphthyl phosphate as a substrate and ph 9.0, three forms of phosphatase activity were revealed; they were arbitrarily called "fast", "intermediate", and "slow" phosphatases. the fast phosphatase has been established to be a fraction of lysosomal acid phosphatase that preserves some low activity at alkaline ph. the question as to which particular class the intermediate p ...200717933343
regulatory circuit based on autogenous activation-repression: roles of c-boxes and spacer sequences in control of the pvuii restriction-modification system.type ii restriction-modification (r-m) systems comprise a restriction endonuclease (rease) and a protective methyltransferase (mtase). after r-m genes enter a new cell, mtase must appear before rease or the chromosome will be cleaved. pvuii and some other r-m systems achieve this delay by cotranscribing the rease gene with the gene for an autogenous transcription activator (the controlling or 'c' protein c.pvuii). this study reveals, through in vivo titration, that c.pvuii is not only an activat ...200717933763
bacterial defluorination of 4-fluoroglutamic acid.fluorinated amino acids are used as enzyme inhibitors, mechanistic probes and in the production of pharmacologically active peptides. because enantiomerically pure 4-fluoroglutamate is difficult to prepare, the selective degradation of the l-isomer is a potentially convenient method of obtaining d-4-fluoroglutamate from the racemate. in this paper, we describe our investigations on the degradation of 4-fluoroglutamate by bacteria. fluoride ion was detected in resting-cell cultures of a number of ...200717901951
pattern of pathogens in ear discharge of hiv-infected children in nnewi, southeast nigeria.this is a descriptive, prospective, hospital-based study designed to determine the prevalent organisms in ear infections and their antimicrobial susceptibility patterns in hiv-infected children compared to age-and sex-matched hiv sero-negative patients.200717902505
purification, crystallization, x-ray diffraction analysis and phasing of an engineered single-chain pvuii restriction endonuclease.the restriction endonuclease pvuii from proteus vulgaris has been converted from its wild-type homodimeric form into the enzymatically active single-chain variant scpvuii by tandemly joining the two subunits through the peptide linker gly-ser-gly-gly. scpvuii, which is suitable for the development of programmed restriction endonucleases for highly specific dna cleavage, was purified and crystallized. the crystals diffract to a resolution of 2.35 a and belong to space group p4(2), with unit-cell ...200717909283
septic postpartum uterine inversion.puerperal uterine inversion is an uncommon but life threatening obstetrical emergency. a 26-year-old woman, para six, was referred from a peripheral hospital seven days after delivery, with a mass protruding per vaginum. complete uterine inversion had occurred after delivery of baby and placenta. she was resuscitated and her genital infection was treated. she had a vaginal hysterectomy upon request. her postoperative recovery was uneventful. poor management of the third stage of labour is a comm ...200717909681
production of l-carnitine by secondary metabolism of bacteria.abstract: the increasing commercial demand for l-carnitine has led to a multiplication of efforts to improve its production with bacteria. the use of different cell environments, such as growing, resting, permeabilized, dried, osmotically stressed, freely suspended and immobilized cells, to maintain enzymes sufficiently active for l-carnitine production is discussed in the text. the different cell states of enterobacteria, such as escherichia coli and proteus sp., which can be used to produce l- ...200717910757
antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent.the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ...200717913083
[the antibacterial activity of fluoroquinolones against gram-negative bacteria isolated from gifu prefecture (2005)].we investigated the susceptibility to 6 fluoroquinolones against 433 strains of gram-negative bacteria isolated from 6 medical facilities in gifu prefecture between january and september in 2005, determined by the agar dilution methods in according with the japan society of chemotherapy. we also investigated the correlation between the degree of resistance to fluoroquinolones and the amino acid substitutions in the quinolone resistance-determining region (qrdr). the tested clinical isolates were ...200717867488
frequency of urinary tract infection in catheterized dogs and comparison of bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with urinary tract infections.to determine frequency of urinary tract infections (utis) in catheterized dogs that had intervertebral disk disease (ivdd) or disease other than ivdd and compare bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with utis.200717867973
synthesis and antimicrobial activity of some novel nucleoside analogues of adenosine and 1,3-dideazaadenosine.a number of nucleoside analogues have been synthesized and evaluated for their antibacterial and antifungal activities against staphylococcus aureus, group d streptococcus, pseudomonas aeruginosa, proteus spp., salmonella spp., aspergillus fumigatus, penicillium marneffei, candida albicans, cryptococcus neoformans, and mucor spp. the compounds 1, 4, and 6 emerged as potent antibacterial agents with mic values of 0.75, 0.38, and 0.19 microm, respectively, against group d streptococcus. further, t ...200717890082
breast abscess bacteriologic features in the era of community-acquired methicillin-resistant staphylococcus aureus epidemics.increasing rates of community-acquired methicillin-resistant staphylococcus aureus (mrsa) infections have also affected the microbial profile of breast abscesses.200717891866
the type iii secretion system of proteus mirabilis hi4320 does not contribute to virulence in the mouse model of ascending urinary tract infection.the gram-negative enteric bacterium proteus mirabilis is a frequent cause of urinary tract infections (utis) in individuals with long-term indwelling catheters or with complicated urinary tracts. the recent release of the p. mirabilis strain hi4320 genome sequence has facilitated identification of potential virulence factors in this organism. genes appearing to encode a type iii secretion system (ttss) were found in a low gc-content pathogenicity island in the p. mirabilis chromosome. this islan ...200717893161
clinical epidemiology of ciprofloxacin-resistant proteus mirabilis isolated from urine samples of hospitalised patients.this study investigated the clinical characteristics of ciprofloxacin-resistant proteus mirabilis isolates from urine samples associated with nosocomial infection or colonisation, and identified the risk-factors for ciprofloxacin resistance. data for patients with ciprofloxacin-resistant p. mirabilis isolates (n=13) were compared with those for randomly selected patients with ciprofloxacin-susceptible p. mirabilis isolates (n=40) who were matched by temporal occurrence as control patients. the m ...200717850340
bacteriology of chronic suppurative otitis media--a multicenter study.bacterial predominance and antibiotic sensitivity have changed over time, making continuous and periodic surveillance necessary in guiding appropriate antibacterial therapy.200717851935
mannose-resistant proteus-like and p. mirabilis fimbriae have specific and additive roles in p. mirabilis urinary tract infections.proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (uti). it produces several types of fimbriae, including mannose-resistant proteus-like (mr/p) fimbriae and p. mirabilis fimbriae (pmf). previously, we determined that these fimbriae affect the ability of p. mirabilis to colonize the urinary tract. the objective of this study was to analyse the effect of the simultaneous lack of p. mirabilis mr/p and pmf fimbriae in uti pathogenesis. a double mutant ...200717854474
acquired ampc type beta-lactamases: an emerging problem in italian long-term care and rehabilitation facilities.we report the multiple detection of proteus mirabilis isolates, from 4 different long-term care and rehabilitation facilities (ltcrfs) of northern italy, resistant to expanded-spectrum cephalosporins and cephamycins and producing an acquired ampc-like beta-lactamase, named cmy-16. genotyping by pfge showed that isolates were clonally related to each other, although not identical. in all isolates the bla(cmy16) gene was not transferable by conjugation and was found to be carried on the chromosome ...200717802913
molecular epidemiology of esbetal producing p. mirabilis strains from a long-term care and rehabilitation facility in italy.we report the detection of multidrug resistant esbetal producing proteus mirabilis isolates from a long-term care and rehabilitation facility (ltcrf) in northern italy. 53% of the collected p. mirabilis strains were esbetal producers. pcr and sequencing techniques confirmed the presence of the bla(tem-92) and bla(cmy-16) resistance genes in 23/26 (88.5%) and 3/26 (11.5%) of the esbetal producers respectively. pfge showed that the tem-92 beta-lactamase producing isolates were not clonally related ...200717802927
polymerase chain reaction for the rapid diagnosis of tuberculous meningitis.polymerase chain reaction (pcr) based on the amplification of a 169 bp dna fragment specific for the mycobacterium tuberculosis complex was evaluated for the rapid diagnosis of tuberculous meningitis (tbm). a total of 105 csf specimens from clinically suspected cases of tbm were studied. clinical details of the cases and cytochemical parameters of the csf specimens were recorded. for pcr 10 csf specimens from cases other than tbm, 4 non-mycobacterial culture isolates (one strain of e coli, one s ...200717802972
occurrence of proteus mirabilis associated with two species of venezuelan oysters.the fecal contamination of raw seafood by indicators and opportunistic pathogenic microorganisms represents a public health concern. the objective of this study was to investigate the presence of enteric bacteria colonizing oysters collected from a venezuelan touristic area. oyster samples were collected at the northwestern coast of venezuela and local salinity, ph, temperature, and dissolved oxygen of seawater were recorded. total and fecal coliforms were measured for the assessment of the micr ...200718157401
[prevalence and bacterial susceptibility of community acquired urinary tract infection in university hospital of brasília, 2001 to 2005].urinary tract infection is among the most common infectious diseases in clinical medicine, and knowledge of its epidemiology and the sensitivity profile of the etiological agents is mandatory. the aim of this study was to identify the most frequent etiological agents and the profile of sensitivity to antimicrobial agents of the bacteria isolated from urine cultures from outpatients at the university hospital of brasília between 2001 and 2005. from analyses at the hospitals microbiology laborator ...200718200417
serological and structural characterization of the o-antigens of the unclassified proteus mirabilis strains tg 83, tg 319, and ccug 10700 (oa).lipopolysaccharide (endotoxin, lps) is an important potential virulence factor of proteus rods. the serological specificity of the bacteria is defined by the structure of the o-polysaccharide chain (o-antigen) of the lps. until now, 76 o-serogroups have been differentiated among proteus strains.200718219766
characterization of antarctic psychrotrophic bacteria with antibacterial activities against terrestrial microorganisms.five-hundreds and eighty bacterial strains, isolated from various antarctic marine sources and locations, were screened for antimicrobial activity against terrestrial microorganisms. twenty-two antarctic isolates (3.8%), mainly retrieved from the water column at terra nova bay (ross sea), expressed antagonistic activity against one to three indicator organisms. escherichia coli and proteus mirabilis resulted as the more susceptible, followed by micrococcus luteus and bacillus subtilis. none of t ...200718072250
[new perspectives of the phage therapy].a solution to the problem of the increasing number of antibiotic-resistant bacterial strains can be the use of rational phage therapy. in the past, bacteriophages (phages) were often used for the treatment and prevention of infections and unlike antibiotic therapy, phage therapy caused almost no serious side effects. while previously several preparations containing whole phage particles were available for phage therapy, currently, the isolation of well characterised and purified phage components ...200718320502
antimicrobial resistance patterns of clinical bacterial isolates in southwestern ethiopia.the aim of this study was to assess the pattern of bacterial resistance to antimicrobial agents in a hospital setting in southwest ethiopia.200718326346
tigecycline in the treatment of complicated intra-abdominal and complicated skin and skin structure infections.tigecycline, a glycylcycline related to the tetracycline class of antibiotics, represents a new option for the treatment of complicated intra-abdominal and complicated skin and skin structure infections. it displays favorable activity in vitro against the most common causative gram-positive, gram-negative and anaerobic pathogens. in addition, tigecycline demonstrates activity against drug-resistant pathogens such as methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, a ...200718516315
epidermal nevus syndromes.the term "epidermal nevus syndrome" (ens) has been used to describe the association of epidermal hamartomas and extra-cutaneous abnormalities. epidermal nevi follow the lines of blaschko. the majority of the extra-cutaneous manifestations involve the brain, eye, and skeletal systems. several subsets with characteristic features have been delineated including the nevus sebaceous syndrome, proteus syndrome, child syndrome, becker nevus syndrome, nevus comedonicus syndrome, and phakomatosis pigment ...200718395670
[synthesis and antimicrobial activity of some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoyl)-d,l-methionine].in order to obtain new compounds with potential antimicrobial activity some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoil)-d,l-methionine have been synthesized. the compounds have been obtained by decyclization reaction of 2-(m-nitrophenyl)-4-(beta-p-methylthioethyl)- delta2-5-oxazolinone with various sulfonamides. the chemical structure of the new sulfonamides was confirmed by elemental analysis data and ir and 1nmr spectral measurements. the compounds were also investigated f ...200718293716
[fundamental study on the selection of antimicrobial prophylactic agents in abdominal surgery].most infections after abdominal operations are endogenous and occur by disseminating bacteria present in the intestinal tract during operation. the risk of developing surgical site infection after abdominal operations is related to the extent of intestinal contamination during operation and to the density and type of bacteria in the intestinal tract. although antimicrobial prophylactic agents must be active against contaminating bacteria during operation, it should not cover all contaminating ba ...200718018416
bacteriology of wound infections in the surgical wards of a teaching hospital in enugu, nigeria.during a 24-month period, january 2001 - december 2002, 404 wound swabs from 390 patients made up of 280 from surgical wounds, 92 from ulcers and 32 from other wounds like lacerations and discharging sinuses were studied. the prevalence of bacterial organisms and their antibiotic susceptibility pattern was analyzed, using standard bacteriologic techniques. two hundred and sixty four organisms (69.8%) were isolated from surgical wounds, 82 (21.7%) from ulcers and 32 (8.5%) from other wounds. coli ...200718564650
confirmation of presumptive salmonella colonies contaminated with proteus swarming using the polymerase chain reaction (pcr) method.in mexico, zero tolerance regulation is practiced regarding salmonella in food products. the presence of which is verified by the procedure described in nom 114-ssa-1994. during the period between august 2002 and march 2003, 245 food samples were tested using this procedure in the central laboratories of the department of health for the state of jalisco (ceeslab). of these 245 samples, 35 showed presumptive colonies contaminated with proteus swarm cells even after selective isolation. these swar ...200718693548
antibacterial activity of some plant extracts used in folk medicine.in the present work, selected plants were screened for their potential antibacterial activity. for evaluating antibacterial activity, both aqueous and organic solvent methanol was used. the plants screened were ocimum sanctum, jatropha gossypifolia, boerhavia diffusa, azadirachta indica, solidago virgaurea, and commelina benghalensis. the antibacterial activity was assessed against six bacterial strains--pseudomonas testosteroni, staphylococcus epidermidis, klebsiella pneumoniae, bacillus subtil ...200718928141
preliminary investigation on the phytochemistry and antimicrobial activity of senna alata l. flower.preliminary studies on the phytochemistry and extracts of water, methanol, chloroform and petroleum ether, of senna alata flowers were examined for antimicrobial properties. extracts tested at a final concentration of 500 microg ml(-1) produced in vitro antimicrobial activities in assays against clinical isolates of staphylococus aureus, candida albicans, escherichia coli, proteus vulgaris, pseudomonans aureginosa and bacillus subtilis. the zones of inhibitions produced by the extracts in agar d ...200719069869
studies on antimicrobial activity of perionyx excavatus.the antimicrobial activity of vermiextract was assessed against four human pathogenic microorganisms. especially strains of proteus species are a prominent cause of urinary tract infection in children. the present investigation deals with particular urinary tract pathogenic organisms like e. coli, proteus, providencia, morganella species. screening was performed by disc diffusion method. the zone of inhibition range from 17 to 19 mm against 1.0x10(8) cells of each culture at 1 mg concentration o ...200719090177
biodegradation and decolourization of anaerobically treated distillery spent wash by a novel bacterial consortium.the aim of this study was to isolate microorganisms capable of decolourizing and degrading anaerobically treated distillery spent wash. a bacterial consortium dmc comprising of three bacterial cultures was selected on the basis of rapid effluent decolourization and degradation, which exhibited 67 +/- 2% decolourization within 24 h and 51 +/- 2% chemical oxygen demand reduction within 72 h when incubated at 37 degrees c under static condition in effluent supplemented with 0.5% glucose, 0.1% kh(2) ...200716473005
antimicrobial, wound healing and antioxidant activities of anthocephalus cadamba.anthocephalus cadamba (roxb.) miq. syn a. chinensis (lamk) a. rich (rubiaceae) is ethnomedicinally widely used in the form of paste by tribe in western ghats for treating skin diseases. in this context, antimicrobial potential of a. cadamba against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing. the alchoholic and aqueou ...200720161916
investigating nanoscale cellular dynamics with cross-sectional spectral domain phase microscopy.we report on cross-sectional imaging of dynamic biological specimens using a spectral domain phase microscopy (sdpm) system capable of operating at a line rate of 19 khz. this system combines the time-sensitive capabilities of sdpm with the multi-point acquisition features of related phase-sensitive techniques. the presented phase portraits and b-scan phase images of spontaneously beating embryonic cardiomyocytes and cytoplasmic flow in a. proteus offer insight into the nature and timing of the ...200719547138
harmonically matched grating-based full-field quantitative high-resolution phase microscope for observing dynamics of transparent biological samples.we have developed a full-field high resolution quantitative phase imaging technique for observing dynamics of transparent biological samples. by using a harmonically matched diffraction grating pair (600 and 1200 lines/mm), we were able to obtain non-trivial phase difference (other than 0 degrees or 180 degrees) between the output ports of the gratings. improving upon our previous design, our current system mitigates astigmatism artifacts and is capable of high resolution imaging. this system al ...200719551112
resin-salve from norway spruce--a potential method to treat infected chronic skin ulcers?the home-made resin salve from norway spruce is traditionally and widely used in folk medicine to heal various skin infections and wounds in northern finland. we have performed laboratory studies to solve the mechanism of resin salve. the resin salve exhibited a bacteriostatic effect against all tested gram-positive bacteria important in human medicine including methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus (vre), but was not effective against gram-nega ...200719356034
the elephant man. 200721991688
the sensitivities to first-line antibiotic therapy of the common urinary tract bacterial infections detected in urine samples at a hospital in metropolitan kampala (uganda).urinary tract infections (utis) are among the most common human infections. many urinary tract bacteria are capable of expressing drug resistance. resistant bacteria may be present from the commencement of the infection or may develop during treatment. this study focused on the problem of antibiotic resistance to the first-line drugs that were used to treat patients presenting with urinary tract infections at rubaga hospital in kampala, uganda.200721499486
antibiotic treatment and resistance of unselected uropathogens in the elderly.a population-based study was conducted among women over the age of 70 years with complaints of uncomplicated urinary tract infections (utis). the positive predictive value of the nitrite test alone or in combination with the leukocyte esterase test ranged between 83% and 99%. the nitrofurantoin prescription rate decreased whereas fluoroquinolone and amoxicillin/clavulanic acid prescriptions increased with increasing age. the aetiology of infection was age-dependent. escherichia coli was the most ...200616473503
functional grouping based on signatures in protein termini.the two ends of each protein are known as the amino (n-) and carboxyl (c-) termini. short signatures in a protein's termini often carry vital cellular function. no systematic research has been conducted to address the importance of short signatures (3 to 10 amino acids) in protein termini at the proteomic level. specifically, it is unknown whether such signatures are evolutionarily conserved, and if so, whether this conservation confers shared biological functions. current signature detection me ...200616475191
the potential use of antibacterial peptide antibody indices in the diagnosis of rheumatoid arthritis and ankylosing spondylitis.both rheumatoid arthritis (ra) and ankylosing spondylitis (as) are potentially disabling arthritic disorders for which as yet no highly sensitive and reliable diagnostic laboratory markers are available.200616484874
detection of diverse bacterial signatures in atherosclerotic lesions of patients with coronary heart disease.bacterial infection has been discussed as a potential etiologic factor in the pathophysiology of coronary heart disease (chd). this study analyzes molecular phylogenies to systematically explore the presence, frequency, and diversity of bacteria in atherosclerotic lesions in patients with chd.200616490835
bacteria and the gastrointestinal tract: beneficial and harmful effects.bacteria are unique microorganisms that have a variety of physiological functions which are beneficial to human beings. however, bacteria can also be harmful and cause infections if translocated from the gastrointestinal tract to the epithelial tissue following surgery. the process of translocation can lead to surgical wounds becoming contaminated with gastrointestinal microbes such as escherichia coli, proteus or klebsiella species. bacteria can also cause severe gastrointestinal infections as ...200616493321
[antimicrobial susceptibility of aerobic bacteria isolated from leishmaniotic ulcers in corte de pedra, ba].a prospective study regarding aspects of antimicrobial susceptibility aspects was realized among patients with tegumentary leishmaniasis in corte de pedra, bahia. cases were composed mainly of adolescent and adult farmer patients with single lesions. staphylococcus aureus predominated (83%) in the cultures with susceptibility to the majority of antibiotics. a mixed bacterial flora in ulcers was encountered in 37 (44.1%) patients. among the gram-negative bacteria isolated, enterobacter sp (13.1%) ...200616501766
choice of antibiotic for empirical therapy of acute cystitis in a setting of high antimicrobial resistance.a high prevalence of antimicrobial resistance among urinary isolates in the garhwal region of uttaranchal.200616505574
mössbauer identification of a protonated ferryl species in catalase from proteus mirabilis: density functional calculations on related models.the proteus mirabilis catalase is one of the most efficient heme-containing catalase and forms a relatively stable compound ii. samples of compound ii were prepared from pmc enriched in (57)fe. for the first time, two different forms of compound ii, namely low ph compound ii (lph ii) (43%) and high ph compound ii (hph ii) (25%), have been characterized by mössbauer spectroscopy at ph 8.3. the ratio lph ii/hph ii increases irreversibly with decreasing ph. the large quadrupole splitting value of l ...200616442627
a new gastrointestinal finding in proteus syndrome: report of a case of multiple colonic hemangiomas.a patient with proteus syndrome presented with lower gastrointestinal bleeding due to multiple colonic hemangiomas, a finding which has not been described previously in this syndrome. the patient was a 20-year-old man with features fulfilling the diagnostic criteria for proteus syndrome. he fulfilled both general criteria (mosaic distribution of the lesions, progressive course and sporadic occurrence) and specific criteria (including epidermal nevus, disproportionate overgrowth of limbs and vasc ...200616445505
factors affecting crystal precipitation from urine in individuals with long-term urinary catheters colonized with urease-positive bacterial species.weekly urinalysis was conducted for 12 weeks on a group of 21 long-term catheter users with confirmed catheter encrustation and urinary tract colonization with urease-positive bacteria, in order to explore the cause of considerable variation in the severity of encrustation between sufferers. the rapidity of catheter blockage correlated significantly with the ph above which crystals precipitated from urine (the nucleation ph) but not the ph of the voided urine itself. linear regression showed the ...200616453146
tryptophanase from proteus vulgaris: the conformational rearrangement in the active site, induced by the mutation of tyrosine 72 to phenylalanine, and its mechanistic consequences.tyr72 is located at the active site of tryptophanase (trpase) from proteus vulgaris. for the wild-type trpase tyr72 might be considered as the general acid catalyst at the stage of elimination of the leaving groups. the replacement of tyr72 by phe leads to a decrease in activity for l-tryptophan by 50,000-fold and to a considerable rearrangement of the active site of trpase. this rearrangement leads to an increase of room around the alpha-c atom of any bound amino acid, such that covalent bindin ...200616455316
isolation of salmonella bacteriophages from swine effluent lagoons.bacteriophages (phages) associated with salmonella were collected from nine swine manure lagoons in mississippi. phages were isolated by an enrichment protocol or directly from effluent. for enrichment, chloroform-treated samples were filtered (0.22 mum) and selectively enriched by adding a cocktail of salmonella strains in trypticase soy broth. after overnight incubation at 35 degrees c, chloroform was added and samples stored at 5 degrees c. enriched samples were tested by double agar layer (d ...200616455853
use of electrochemical dna biosensors for rapid molecular identification of uropathogens in clinical urine specimens.we describe the first species-specific detection of bacterial pathogens in human clinical fluid samples using a microfabricated electrochemical sensor array. each of the 16 sensors in the array consisted of three single-layer gold electrodes-working, reference, and auxiliary. each of the working electrodes contained one representative from a library of capture probes, each specific for a clinically relevant bacterial urinary pathogen. the library included probes for escherichia coli, proteus mir ...200616455913
crystallization and preliminary x-ray diffraction analysis of a cold-adapted catalase from vibrio salmonicida.catalase (ec 1.11.1.6) catalyses the breakdown of hydrogen peroxide to water and molecular oxygen. recombinant vibrio salmonicida catalase (vsc) possesses typical cold-adapted features, with higher catalytic efficiency, lower thermal stability and a lower temperature optimum than its mesophilic counterpart from proteus mirabilis. crystals of vsc were produced by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. the crystals belong to the monoclinic space group p2(1) ...200616511268
gastric microbiota in elderly patients fed via nasogastric tubes for prolonged periods.aspiration of oropharyngeal contents is a constant threat for elderly patients fed via a nasogastric tube (ngt). colonization of the oropharynx of these patients by pathological flora and the development of biofilms on the feeding tube has been documented recently. in addition, the presence of the ngt may interfere with the gastro-oesophageal sphincter, leading to gastro-oesophageal reflux. thus, the passage of pathogenic bacteria in both directions is facilitated. the purpose of this study was ...200616516342
evaluation of the phoenix 100 id/ast system and nid panel for identification of enterobacteriaceae, vibrionaceae, and commonly isolated nonenteric gram-negative bacilli.the phoenix 100 id/ast system (becton dickinson co., sparks, md.) is an automated system for the identification and antimicrobial susceptibility testing of bacterial isolates. this system with its negative identification (nid) panel was evaluated for its accuracy in the identification of 507 isolates of the family enterobacteriaceae, 57 other nonenteric gram-negative isolates that are commonly isolated in clinical microbiology laboratories, and 138 isolates of the family vibrionaceae. all of the ...200616517878
global assessment of the antimicrobial activity of polymyxin b against 54 731 clinical isolates of gram-negative bacilli: report from the sentry antimicrobial surveillance programme (2001-2004).in total, 54 731 gram-negative bacilli isolated worldwide between 2001 and 2004 from diverse sites of infection were tested for susceptibility to polymyxin b by the broth reference microdilution method, with interpretation of results according to clsi (formerly nccls) guidelines. polymyxin b showed excellent potency and spectrum against 8705 pseudomonas aeruginosa and 2621 acinetobacter spp. isolates (mic50, < or = 1 mg/l and mic90, 2 mg/l for both pathogens). polymyxin b resistance rates were s ...200616524407
[proteus syndrome with cerebral vascular malformations].proteus syndrome is a congenital hamartomatous dysplasia. this sporadic disorder involves the skeletal system, soft tissues, skin and vascular system. the most likely pathogenesis involves somatic mosaicism. main manifestations included soft-tissue and epidermal nevi, partial gigantism, hemihypertrophy, exostoses, lipomas and vascular anomalies. the most common brain abnormalities are hemimegencephaly and migrational disorders. we present a case of proteus syndrome with cerebral vascular anomali ...200616525914
[protective effect of the aerosol immunization of mice with the polycomponent vaccine immunovac vp-4 after the challenge of the animals with klebsiella pneumoniae virulent strain].used four schemes of the administration of the preparation with different time of the exposition of the animals in an aerosol chamber were tested with their subsequent intraperitoneal challenge with k. pneumoniae virulent strain k16. irrespective of the number of immunization courses, the administration of the preparation made at intervals of 1 day, or daily, did not ensure any protective effect, but only led to an insignificant increase in their survival time in comparison with nonimmunized ani ...200616532635
optimization of ferrioxamine e concentration as effective supplementation for selective isolation of salmonella enteritidis in egg white.utilization of ferrioxamine e (fe) as a sole source of iron distinguishes salmonella from a number of related species, including escherichia coli. fe is not able to serve as a source of iron for e. coli or the proteus-providencia-morganella group. this confers a selective advantage on salmonella enteritidis in egg white supplemented with fe. the optimum concentration of fe that promoted a selective advantage for salmonella in egg white was determined. four supplementation concentrations were eva ...200616541696
isolation of enterobacter sakazakii from stable flies, stomoxys calcitrans l. (diptera: muscidae).enterobacter sakazakii is an opportunistic foodborne pathogen that causes meningitis, enterocolitis, and sepsis, primarily in immunocompromised infants. previously, it was suggested that stable flies, stomoxys calcitrans, were a vector or reservoir of this pathogen. in our study, by means of a culturing approach combined with 16s rdna pcr-restriction fragment length polymorphism genotyping and sequencing, we screened 928 individual stable flies collected in kansas and florida. two stable flies ( ...200616541702
increasing trend in the prevalence of plasmid-mediated ampc beta-lactamases in enterobacteriaceae lacking chromosomal ampc gene at a korean university hospital from 2002 to 2004.the aim of the study is to investigate the prevalence of plasmid-mediated ampc beta-lactamases in enterobacteriaceae naturally lacking chromosomal ampc beta-lactamases. a total of 1860 clinical isolates of klebsiella spp., salmonella spp., and proteus mirabilis were collected from a korean hospital between january 2002 and december 2004. for the isolates that are nonsusceptible to cefoxitin, polymerase chain reaction amplification of the bla(shv), bla(tem), and bla(ampc) genes and sequencing wer ...200616545935
effect of steroid on mitochondrial oxidative stress enzymes, intestinal microflora, and bacterial translocation in rats subjected to temporary liver inflow occlusion.protective effects of steroids against ischemia-reperfusion (i/r) injury are well known, but there is little information about the influence of temporary inflow occlusion on intestinal barrier function or bacterial translocation. the aim of this experimental study was to investigate the effects on liver, kidney, spleen, ileal mitochondrial stress enzymes, and bacterial translocation of methylprednisolone (mp) in rats undergoing temporary liver inflow occlusion. twenty-seven pathogen-free wistar ...200616549125
bioactive components of the traditionally used mushroom podaxis pistillaris.in the course of an ethnobotanical study on fungi used in yemeni ethnomedicine the fungus podaxis pistillaris (podaxales, podaxaceae, basidiomycetes) was found to exhibit antibacterial activity against staphylococcus aureus, micrococcus flavus, bacillus subtilis, proteus mirabilis, serratia marcescens and escherichia coli. in the culture medium of p. pistillaris three epidithiodiketopiperazines were identified by activity-guided isolation. based on spectral data (nmr, esi-ms and dci-ms) their id ...200616550228
the presence of a conjugative gram-positive tn2009 in gram-negative commensal bacteria.to determine whether mef(a)-msr(d) and tet(m) genes are linked in representative gram-negative isolates and/or transferred together during conjugation. to molecularly characterize the acinetobacter junii element and compare the structure and sequence with the non-conjugative streptococcus pneumoniae tn2009 element.200616551693
urinary tract infections in general practice patients: diagnostic tests versus bacteriological culture.urinary tract infections (utis) are common bacterial infections encountered in general practice. for the optimal treatment the general practitioner (gp) should rely on the results of diagnostic tests and recent antimicrobial susceptibility of uropathogens.200616554337
proteus syndrome with huge tonsillar mass causing dysphagia: a rare case.proteus syndrome is a rare and highly variable hamartomatous syndrome that can affect multiple organ systems. it is characterized by hyperplasia of connective tissue, vascular malformations, epidermal naevi and hyperostosis. most of the cases present to plastic and orthopaedic surgeons. otolaryngologic presentations are minimal. we report such a rare case, which presented with a huge unilateral tonsillar mass causing dysphagia.200616556346
pharmacologic characteristics of prulifloxacin.prulifloxacin, the prodrug of ulifloxacin, is a broad-spectrum oral fluoroquinolone antibacterial agent. after absorption, prulifloxacin is metabolized by esterases to ulifloxacin. the drug has a long elimination half-life, allowing once-daily administration. in vitro, ulifloxacin is generally more active than other fluoroquinolones against a variety of clinical isolates of gram-negative bacteria, including community and nosocomial isolates of escherichia coli, klebsiella spp., proteus, providen ...200616360331
susceptibility of recent bacterial isolates to cefdinir and selected antibiotics among children with urinary tract infections.cefdinir, an extended-spectrum cephalosporin administered orally, is approved by the u.s. federal drug administration for treatment of skin and respiratory tract infections. during the last two years at the authors' institution, this agent has been used as an off-label treatment for urinary tract infections in children.200616365328
quantitative bacterial micro-assay for rapid detection of serum phenylalanine on dry blood-spots: application in phenylketonuria screening.phenylketonuria is an inherited metabolic disease, which is characterized by an increased level of serum phenylalanine. quantitative measurement of phenylalanine in the serum of phenylketonuria patients is necessary to confirm the disease, and to distinguish phenylketonuria from other forms of hyperphenylalaninemia. in this study, we report a rapid and inexpensive micro-assay for simultaneous detection and quantitative measurement of serum phenylalanine on dry blood-spots. analysis of the standa ...200616375590
bacteria isolated from the different developmental stages and larval organs of the obligate parasitic fly, wohlfahrtia magnifica (diptera: sarcophagidae).wohlfahrtia magnifica (diptera: sarcophagidae) is the major myiasis-causing fly species in the whole of eurasia for most important domestic animals. the aim of the present work was to obtain data on the culturable bacteria isolated under aerobic conditions from this fly: bacteria were isolated from all developmental stages (larvae, pupa, and imago) of wohlfahrtia magnifica, and the third-stage larval organs were also sampled. to determine the possible antagonistic effects between the dominant ba ...200616382282
antimicrobial activities of silver dressings: an in vitro comparison.a range of silver-coated or -impregnated dressings are now commercially available for use but comparative data on their antimicrobial efficacies are limited. the antibacterial activities of five commercially available silver-coated/impregnated dressings were compared against nine common burn-wound pathogens, namely methicillin-sensitive and -resistant staphylococcus aureus (mrsa), enterococcus faecalis, pseudomonas aeruginosa, escherichia coli, enterobacter cloacae, proteus vulgaris, acinetobact ...200616388031
purification, properties and extended solution structure of the complex formed between human immunoglobulin a1 and human serum albumin by scattering and ultracentrifugation.immunoglobulin a (iga) is unique amongst antibodies in being able to form polymeric structures that may possess important functions in the pathology of specific diseases. iga also forms complexes with other plasma proteins, the iga1-human serum albumin (hsa) complex (iga1-hsa) being typical. we have purified this complex using a novel two-step purification based on thiophilic chromatography and gel filtration, and characterised this. hsa is linked covalently to the tailpiece of iga1 by a disulph ...200616376934
novel plasmid-mediated 16s rrna methylase, rmtc, found in a proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides.proteus mirabilis ars68, which demonstrated a very high level of resistance to various aminoglycosides, was isolated in 2003 from an inpatient in japan. the aminoglycoside resistance of this strain could not be transferred to recipient strains escherichia coli csh-2 and e. coli hb101 by a general conjugation experiment, but e. coli dh5alpha was successfully transformed by electroporation with the plasmid of the parent strain, ars68, and acquired an unusually high degree of resistance against ami ...200616377684
proteus syndrome with multiple genitourinary abnormalities.proteus syndrome is a rare hamartomatous disorder comprising a broad spectrum of congenital malformations and overgrowth of multiple tissues. some rare urogenital malformations have been reported before. this case is unique for the presence of multiple genitourinary anomalies including retroperitoneal cystic mass, intra-abdominal testicle with hematoma and ureterovesical stricture apart from common clinic findings of proteus syndrome.200616401930
dissemination of proteus mirabilis isolates harboring ctx-m-14 and ctx-m-3 beta-lactamases at 2 hospitals in taiwan.from february to june 2003, 111 clinical isolates of proteus mirabilis were mainly isolated from patients with respiratory or urinary tract infections hospitalized at 3 district hospitals (a, b, c) in central taiwan. among them, 34 (30.6%) strains, isolated within 2 hospitals (a and b), exhibited nonsusceptibility to cefotaxime with significant reduction of mic (> or = 3 log2 dilution) by the effect of clavulanic acid, which confirmed the phenotype of extended-spectrum beta-lactamases (esbls). t ...200616406185
rigid sigmoidoscopy: a potential hazard for cross-contamination.rigid sigmoidoscopy using a disposable or nondisposable sigmoidoscope is a common outpatient procedure. it has been assumed that the nondisposable bellows and light head of the sigmoidoscope remain free from enteric organisms so that the procedure is sterile if a disposable or nondisposable (metal) sigmoidoscope shaft is used. the aim of this study was to identify the presence of organisms within the bellows or light head of the sigmoidoscope.200616418794
perioperative respiratory complications caused by cystic lung malformation in proteus syndrome.a 7-year-old girl with proteus syndrome, complicated with severe kyphoscoliosis and cystic lung malformation, developed respiratory complications during and after posterior spinal fusion under general anesthesia. we speculated that low oxygenation while in the prone position contributed to ventilation perfusion mismatch owing to the cystic lung malformation that existed predominantly in the right lung, and that postoperative respiratory failure was caused by initially increased bronchial mucous ...200616421672
the first detection of ctx-m-14 extended-spectrum beta-lactamase among diverse beta-lactamase-producing proteus mirabilis clinical isolates.ctx-m-14- and -2-like extended-spectrum beta-lactamases were detected in 5 and 1 proteus mirabilis isolates, respectively, among 92 non-duplicate strains tested from december 2002 to september 2003. this is the first report of the ctx-m-14 enzyme in p. mirabilis to the best of our knowledge and suggests that the ctx-m-type enzyme is gradually spreading to this species. bla(tem), bla(shv-1), and/or bla(oxa-10) alleles were also detected in 35 ampicillin-resistant isolates tested.200616427241
radiographic and ultrasonographic findings of emphysematous cystitis in four nondiabetic female dogs.the urinary bladder of four dogs with emphysematous cystitis was assessed radiographically. ultrasonography was also performed using a 7.5-mhz microconvex probe in dorsal recumbency and in a standing position. ultrasonographically there were bright echoes and reverberations typical of gas in all dogs. this was entrapped in the bladder wall as it appeared in the same location in recumbent and standing positions. bladder size was reduced and bladder content was echogenic in all dogs. in only one o ...200616429992
characterization of the brewery spoilage bacterium obesumbacterium proteus by automated ribotyping and development of pcr methods for its biotype 1.to study the ability of automated ribotyping to characterize obesumbacterium proteus and hafnia alvei, to design primers and to evaluate standard end-point and real-time pcr for the detection of o. proteus biotype 1 in beer and in brewers's yeast-containing samples.200616430517
evaluation of a novel kit for the rapid detection of extended-spectrum beta-lactamases.since a method of rapidly detecting extended-spectrum beta-lactamase (esbl) production in gram-negative isolates from patients with severe infection is urgently required, the present study of a novel commercial kit was conducted. the cica-beta test i (kanto chemical, tokyo, japan) is designed for the rapid detection of esbl in gram-negative bacteria directly from isolated colonies in a 15-min protocol. in this study, a total of 304 strains of klebsiella spp., escherichia coli and proteus mirabil ...200616416268
comparative analysis of a modified ecolite method, the colicomplete method, and a most-probable-number method for detecting escherichia coli in orange juice.the ecolite high volume juice (hvj) presence-absence method for a 10-ml juice sample was compared with the u.s. food and drug administration bacteriological analytical manual most-probable-number (mpn) method for analysis of artificially contaminated orange juices. samples were added to ecolite-hvj medium and incubated at 35 degrees c for 24 to 48 h. fluorescent blue results were positive for glucuronidase- and galactosidase-producing microorganisms, specifically indicative of about 94% of esche ...200616416921
surgical treatment of an unusual patient with proteus syndrome. 200616432429
cmy-16, a novel acquired ampc-type beta-lactamase of the cmy/lat lineage in multifocal monophyletic isolates of proteus mirabilis from northern italy.we report multifocal detection (four different cities in northern italy) of proteus mirabilis isolates resistant to both oxyimino- and 7-alpha-methoxy-cephalosporins and producing a novel acquired ampc-like beta-lactamase. the enzyme, named cmy-16, is a variant of the cmy/lat lineage, which differs from the closest homologues, cmy-4 and cmy-12, by a single amino acid substitution (a171s or n363s, respectively) and from cmy-2 by two substitutions (a171s and w221r). expression of the cloned bla(cm ...200616436718
cowden disease and lhermitte-duclos disease: an update. case report and review of the literature.cowden disease is a rare autosomal-dominant phacomatosis and cancer syndrome that is associated with lhermitte-duclos disease (ldd), also called dysplastic cerebellar gangliocytoma.200616459996
clonal occurrences of multidrug-resistant gram-negative bacilli: report from the meropenem yearly susceptibility test information collection surveillance program in the united states (2004).the meropenem yearly susceptibility test information collection program is a global, longitudinal resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of bacterial pathogens to carbapenems and other broad-spectrum agents. between 1999 and 2004, a total of 10-16 us medical centers referred up to 200 nonduplicate isolates from clinical infections to a central processing laboratory. over this 6-year period, the antimicrobial activity of 12 broad-s ...200616466890
clinical and economic impact of bacteremia with extended- spectrum-beta-lactamase-producing enterobacteriaceae.we studied outcomes of extended-spectrum beta-lactamase (esbl) production in enterobacteriaceae bacteremia. inpatients with bacteremia caused by esbl-producing escherichia coli, klebsiella spp., or proteus spp. (cases) were compared with patients with bacteremia caused by non-esbl producers (controls). outcomes included mortality, mortality due to infection, length of stay (los), delay in appropriate therapy (dat), discharge to a chronic care facility, and hospital cost. ninety-nine cases and 99 ...200616569837
in vitro antibacterial, antifungal & cytotoxic activity of some isonicotinoylhydrazide schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes.isonicotinoylhydrazide schiff's bases formed by the reaction of substituted and unsubstituted furyl-2-carboxaldehyde and thiophene-2-carboxaldehyde with isoniazid and, their co (ii), cu (ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against mycobacterium tuberculosis, escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebac ...200616570512
antibiotic susceptibility tests directly on urine samples using uro-quick, a rapid automated system.during the period june 2003-march 2004, 12,579 urine samples were examined employing the uro-quick system. positive samples (1,948) were subsequently gram-stained and processed by standard procedures for microorganism identification and antibiotic susceptibility determination by the disk diffusion method. results of this latter test were compared with those obtained employing the new rapid uro-quick method. antibiotics were introduced in vials containing 2 ml of mueller-hinton broth, then 0.5 ml ...200616572888
modulation of crystalline proteus mirabilis biofilm development on urinary catheters.the crystalline biofilms formed by proteus mirabilis can seriously complicate the care of patients undergoing long-term bladder catheterization. the generation of alkaline urine by the bacterial urease causes calcium and magnesium phosphates to precipitate from urine and accumulate in the catheter biofilm, blocking the flow of urine from the bladder. the ph at which these salts crystallize from a urine sample, the nucleation ph (ph(n)), can be elevated by diluting the urine and by increasing its ...200616585633
antibiotic-lock therapy for long-term intravascular catheter-related bacteraemia: results of an open, non-comparative study.infection is one of the main problems associated with long-term central venous catheters. this study assesses the effectiveness of antibiotic-lock therapy (alt) for treating catheter-related bacteraemia (crb).200616597634
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