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effects of proteus mirabilis lipopolysaccharides with different o-polysaccharide structures on the plasma membrane of human erythrocytes.the effects of o33 and o49 p. mirabilis lipopolysaccharides (lpss) on human erythrocyte membrane properties were examined. physical parameters of the plasma membrane, such as membrane lipid fluidity, physical state of membrane proteins, and osmotic fragility, were determined. the fluidity of the lipids was estimated using three spin-labeled stearic acids of doxyl derivatives: 5-doxylstearic acid, 12-doxylstearic acid, and 16-doxylstearic acid. all the applied labels locate to different depths of ...200818669036
tigecycline: a critical update.tigecycline is the first food and drug administration (fda) approved glycylcycline antibiotic. it has shown remarkable in vitro activity against a wide variety of gram-positive, gram-negative and anaerobic bacteria including many multidrug resistant (mdr) strains. however, it has minimal activity against pseudomonas aeruginosa and proteus spp. to date, little resistance to tigecycline has been reported. clinical trials studying complicated skin and skin-structure infections (csssis) demonstrated ...200818676218
proteus syndrome complicated by multiple spinal meningiomas.proteus syndrome is a complex and highly variable disorder comprising malformations and overgrowth of multiple tissues. we present a 65-year-old japanese man who had multiple spinal meningiomas and accompanying neural symptoms. his right leg showed hypertrophy with cerebriform connective-tissue naevus on the sole, and macrodactyly. chest computed tomography imaging revealed mild cystic and emphysematous lung changes, which were possibly related to proteus syndrome. otherwise, he had no particula ...200818681877
[intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees].dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ...200818683661
the pathological newborn in small animals: the neonate is not a small adult.the rate of perinatal death is variable but highest during parturition, immediately after birth and in the first days of life. infectious diseases, above all bacterial, are the second most important cause of mortality after losses during parturition. a lot of factors are involved predisposing puppies and kittens to bacterial infections: respiratory distress, hypothermia, hypoglycaemia, dehydration, congenital abnormalities. e. coli, streptococci and staphylococci, klebsiella sp., pseudomonas sp. ...200818688749
[current issues related to nosocomial infections in general surgery].the authors analyze the prevalence of nosocomial infections in their facility between 1999 and 2006. the annual rates ranged from 1.92 % to 4.24 %, with an average prevalence of 3.12 %. the most frequent were wound infections, febrile conditions associated with positive blood culture and urinary tract infections. in nosocomial infections, the average length of hospitalization increased by a factor of 3.5 and treatment costs rose. sporadically, multiresistant strains were detected, such as mrsa, ...200818688770
dna fragmentation in microorganisms assessed in situ.chromosomal dna fragmentation may be a direct or indirect outcome of cell death. unlike dna fragmentation in higher eukaryotic cells, dna fragmentation in microorganisms is rarely studied. we report an adaptation of a diffusion-based assay, developed as a kit, which allows for simple and rapid discrimination of bacteria with fragmented dna. intact cells were embedded in an agarose microgel on a slide, incubated in a lysis buffer to partially remove the cell walls, membranes, and proteins, and th ...200818689511
the aac(6')ib gene in proteus mirabilis strains resistant to aminoglycosides.the aim of this study was to evaluate the presence of aac(6')-ib gene conferring resistance to aminoglycosides in proteus mirabilis strains. five isolates had aac(6')-ib gene. in one case the gene was no-expressed. three isolates were resistant to all aminoglycosides and minimum inhibitory concentrations were > or = 256 microg/ml. additionally, all positive strains were resistant to tetracycline and ciprofloxacin.200819141409
[antimicrobial activity of soft and purified propolis extracts].to evaluate the antimicrobial activity of soft and purified propolis extracts. study object and methods: antimicrobial activity of soft and purified propolis extracts was determined with reference cultures of staphylococcus aureus atcc 25923, enterococcus faecalis atcc 29212, escherichia coli atcc 25922, klebsiella pneumoniae atcc 33499, pseudomonas aeruginosa atcc 27853, proteus mirabilis atcc 12459, bacillus subtilis atcc 6633, bacillus cereus atcc 8035, and fungus candida albicans atcc 60193. ...200819142056
laboratory detection of extended-spectrum beta-lactamase by an automated system.this study aims to evaluate the positive predictive value (ppv) and the negative predictive value (npv) of vitek2 in detecting extended-spectrum beta-lactamase (esbl) phenotypes when compared to a manual confirmatory test as gold standard. a sample of escherichia coli, klebsiella spp and proteus mirabilis isolates were collected by 5 laboratories in the emilia-romagna region (italy). vitek2 appears to be an accurate tool to detect esbl phenotypes of e. coli isolates; some concern remains about i ...200819123314
[antagonistic properties of enterococci isolated from gastrointestinal tract of long-livers].the strains with antagonistic activity against indicator strains pseudomonas aeruginosa, proteus vulgaris, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumonia, salmonella enterica were revealed among 58 enterococcus strains isolated from gastrointestinal tract of long-livers. the influence of different components of mrs medium on the antagonistic effect was established. antagonistic activity remained on the medium without glucose in 36% of strains, on the me ...200819044009
hyperammonemic encephalopathy caused by urinary tract infection with urinary retention. 200819046708
antimicrobial resistance and prevalence of canine uropathogens at the western college of veterinary medicine veterinary teaching hospital, 2002-2007.between january 2002 and june 2007, uropathogens were isolated from 473 of 1557 canine urine samples submitted to prairie diagnostic services from the western college of veterinary medicine veterinary teaching hospital. culture and susceptibility results were analyzed, retrospectively, to estimate the prevalence of common bacterial uropathogens in dogs with urinary tract infections and to identify changes in antimicrobial resistance. the most common pathogens identified were escherichia coli, st ...200819119366
comparison of solid-phase cytometry and the plate count method for the evaluation of the survival of bacteria in pharmaceutical oils.to compare the survival of four bacterial strains (escherichia coli, proteus mirabilis, staphylococcus aureus, pseudomonas aeruginosa) in pharmaceutical oils, including jojoba oil/tea tree oil, carbol oil, jojoba oil and sesame oil.200819120928
biotransformation of the marine sesquiterpene pacifenol by a facultative marine fungus.continued investigation of the marine alga laurencia claviformis has led to the isolation of pacifenol, a halogenated sesquiterpene as the major metabolite. the microbial transformation of pacifenol is reported. it was cultivated with a marine strain of penicillium brevicompactum yielding a new compound. the structure was elucidated on the basis of spectroscopic data. the anti-microbial activity of pacifenol derivatives is reported.200819085420
involvement of myosin vi immunoanalog in pinocytosis and phagocytosis in amoeba proteus.recently, we found a 130-kda myosin vi immunoanalog in amoeba, which bound to actin in an atp-sensitive manner and in migrating amoebae colocalized to filamentous actin and dynamin ii-containing vesicular structures. to further characterize this protein, we assessed its involvement in amoeba pinocytosis and phagocytosis. confocal immunofluorescence microscopy and electron microscopy of immunogold-stained cells revealed that, in pinocytotic and phagocytotic amoebae, the myosin vi immunoanalog was ...200819088799
comparison of pcr-based molecular markers for the characterization of proteus mirabilis clinical isolates.proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. for epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (pfge) or ribotyping, have been developed for this pathogen. however, these methods are labor intensive a ...200819219283
[occupational exposure to mesophilic microorganisms associated with commercial processing of compost for mushroom production].the aim of the study was to assess the occupational exposure to mesophilic microorganisms associated with commercial processing of compost for mushroom production.200819227882
antimicrobial activity of some macrophytes from lake manzalah (egypt).the antimicrobial activities of aqueous and organic solvents (chloroform, ethanol and methanol) extracts of four plants ceratophyllum demersum l., eichhornia crassipes, potamogeton crispus and potamogeton pectinatus were tested in vitro against seventeen different microorganisms including gram-positive and gram-negative bacteria and fungi. nine of these identified organisms were obtained from different sources, bacillus subtilis 1020, bacillus cereus 1080, staphylococcus aureus, erwinia carotovo ...200819205264
[characteristic of resistance to beta-lactam antibiotics of nosocomial strains of proteus mirabilis].antibiotic resistance of bacterium proteus mirabilis isolated from inpatients was assessed in 30 medical centres from 15 regions of russia. the most active antibacterial preparations remained carbapenems (imipenem and meropenem). between 3rd generation cephalosporins, the lowest minimal inhibitory concentration (mic) was observed in inhibition-protected preparations (ceftazidime/clavulanate: mic50 < 0.125 mcg/ml, mic90 128 mcg/ml; cefoperazone/sulbactam: mic50 2 mcg/ml, mic90 64 mcg/ml). detecti ...200819189465
susceptibility pattern of some clinical bacterial isolates to selected antibiotics and disinfectants.the antibacterial activities of five antibiotics, three brands of ofloxacins (obenasin, floxavid and drovid) and two brands of ciprofloxacins (uroxin and siprosan), and five commonly used disinfectants (lysol, dettol, purit, roberts and wex-cide) against staphylococcus aureus, escherichia coli, proteus spp., pseudomonas aeruginosa, streptococcus spp. and bacillus spp. were investigated. the growth inhibitory effect of both the antibiotics and disinfectants were determined using paper disk diffus ...200819004240
antimicrobial resistance among uropathogens responsible for community-acquired urinary tract infections in an italian community.this study aimed to establish the pattern of the antimicrobial resistance among the leading uropathogens causing community-acquired utis in an area of the region of apulia, southern italy. twenty-one thousand and two hundred outpatients, 6,893 males and 14,307 females, were enrolled. urinary isolates were identified by conventional methods and the susceptibility to 18 antimicrobials determined. recognized uropathogens escherichia coli, klebsiella pneumoniae and proteus mirabilis were isolated fr ...200819129070
[clinical analysis of otogenic intracranial complications].to analyze the clinical features and treatment protocols of otogenic intracranial complications in peking union medical college hospital.200819267967
[etiology of urinary tract infections and antimicrobial susceptibility: a study conducted on a population of children hospitalized in the department of pediatrics at warsaw bielany hospital; 2004-2006].the basis of urinary tract infection (uti) diagnosis in children is based on positive results of microbiological tests. the selection of an antimicrobial drug for empirical treatment and prophylaxis of uti is determined by the susceptibility of bacterial strains isolated from urine cultures.200819418948
antibacterial profile of fermented seed extracts of ricinus communis: findings from a preliminary analysis.the study was carried out to ascertain the antibacterial properties inherent in fermented seed extracts of ricinus communis. dry seeds of r. communis (castor oil plant) were deshelled, grounded to powder, fermented, and then extracted both with alcohol and water using soxhlet machine. different concentrations of the extracts were tested against selected bacteria using diffusion method of susceptibility testing on sensitivity testing agar medium. klebsiella pneumoniae, escherichia coli, proteus v ...200819434215
[preliminary data on the utilization of monural to treat pregnant women with asymptomatic bacteriuria]. 200819452625
[epidemiology of bacterial intrahospital infections in newborns].nosocomial infections are important problem for the modern medicine. especially sensitive category, susceptible to nosocomial infections, are the infants. the aim of the research was to establish an active monitoring over the breaking out of the nosocomial infections in the neonatology department of the gynecology and obstetrics clinic of the university clinical center tuzla (ukc) and to determine the kind of bacteria that causes the nosocomial infections as well as the type of infection accordi ...200819469274
investigating nanoscale cellular dynamics with cross-sectional spectral domain phase microscopy.we report on cross-sectional imaging of dynamic biological specimens using a spectral domain phase microscopy (sdpm) system capable of operating at a line rate of 19 khz. this system combines the time-sensitive capabilities of sdpm with the multi-point acquisition features of related phase-sensitive techniques. the presented phase portraits and b-scan phase images of spontaneously beating embryonic cardiomyocytes and cytoplasmic flow in a. proteus offer insight into the nature and timing of the ...200719547138
harmonically matched grating-based full-field quantitative high-resolution phase microscope for observing dynamics of transparent biological samples.we have developed a full-field high resolution quantitative phase imaging technique for observing dynamics of transparent biological samples. by using a harmonically matched diffraction grating pair (600 and 1200 lines/mm), we were able to obtain non-trivial phase difference (other than 0 degrees or 180 degrees) between the output ports of the gratings. improving upon our previous design, our current system mitigates astigmatism artifacts and is capable of high resolution imaging. this system al ...200719551112
resin-salve from norway spruce--a potential method to treat infected chronic skin ulcers?the home-made resin salve from norway spruce is traditionally and widely used in folk medicine to heal various skin infections and wounds in northern finland. we have performed laboratory studies to solve the mechanism of resin salve. the resin salve exhibited a bacteriostatic effect against all tested gram-positive bacteria important in human medicine including methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus (vre), but was not effective against gram-nega ...200719356034
studies on antimicrobial activity of perionyx excavatus.the antimicrobial activity of vermiextract was assessed against four human pathogenic microorganisms. especially strains of proteus species are a prominent cause of urinary tract infection in children. the present investigation deals with particular urinary tract pathogenic organisms like e. coli, proteus, providencia, morganella species. screening was performed by disc diffusion method. the zone of inhibition range from 17 to 19 mm against 1.0x10(8) cells of each culture at 1 mg concentration o ...200719090177
confirmation of presumptive salmonella colonies contaminated with proteus swarming using the polymerase chain reaction (pcr) method.in mexico, zero tolerance regulation is practiced regarding salmonella in food products. the presence of which is verified by the procedure described in nom 114-ssa-1994. during the period between august 2002 and march 2003, 245 food samples were tested using this procedure in the central laboratories of the department of health for the state of jalisco (ceeslab). of these 245 samples, 35 showed presumptive colonies contaminated with proteus swarm cells even after selective isolation. these swar ...200718693548
antibacterial activity of some plant extracts used in folk medicine.in the present work, selected plants were screened for their potential antibacterial activity. for evaluating antibacterial activity, both aqueous and organic solvent methanol was used. the plants screened were ocimum sanctum, jatropha gossypifolia, boerhavia diffusa, azadirachta indica, solidago virgaurea, and commelina benghalensis. the antibacterial activity was assessed against six bacterial strains--pseudomonas testosteroni, staphylococcus epidermidis, klebsiella pneumoniae, bacillus subtil ...200718928141
preliminary investigation on the phytochemistry and antimicrobial activity of senna alata l. flower.preliminary studies on the phytochemistry and extracts of water, methanol, chloroform and petroleum ether, of senna alata flowers were examined for antimicrobial properties. extracts tested at a final concentration of 500 microg ml(-1) produced in vitro antimicrobial activities in assays against clinical isolates of staphylococus aureus, candida albicans, escherichia coli, proteus vulgaris, pseudomonans aureginosa and bacillus subtilis. the zones of inhibitions produced by the extracts in agar d ...200719069869
fournier's gangrene: ten-year experience in a medical center in northern taiwan.fournier's gangrene is a life-threatening infection. the mortality is still high despite the rapid advancement of modern intensive care and surgical technique. in this study, we present our institution's recent experience with a large series of patients with fournier's gangrene.200718087630
microorganisms present in discharging otitis media in a group of patients in kathmandu.discharge from the ear is one of the commonest symptoms of infections of the ear. to study the major strains of bacteria encountered in ear discharges, this study was conducted from july 1999, to june 2001 in the microbiology laboratory of om hospital. a total of 115 patients, of 6-35 years of age clinically diagnosed as discharging otitis media were studied. in 60.9% single organism and in 26.08% two organisms were isolated. results revealed that, staphylococcus aureus (34.3%), klebsiella pneum ...200718092440
characterization of antarctic psychrotrophic bacteria with antibacterial activities against terrestrial microorganisms.five-hundreds and eighty bacterial strains, isolated from various antarctic marine sources and locations, were screened for antimicrobial activity against terrestrial microorganisms. twenty-two antarctic isolates (3.8%), mainly retrieved from the water column at terra nova bay (ross sea), expressed antagonistic activity against one to three indicator organisms. escherichia coli and proteus mirabilis resulted as the more susceptible, followed by micrococcus luteus and bacillus subtilis. none of t ...200718072250
[attention-getting cross infections: extended-spectrum beta-lactamase-producing bacterial infections]. 200718069300
antibiotic susceptibility and molecular characterisation of proteus mirabilis isolates in hospitals from the west pomeranian area of poland.proteus mirabilis isolates (n = 177), collected between 1996 and 2000 in four hospitals in the west pomeranian area of poland, were characterized by antibiotype and pulsed-field gel electrophoresis (pfge). the selected isolates were collected from different wards (intensive care unit, surgery, internal medicine, and urology). the strains were cultured from various specimen types, mostly from urine, wound samples, bronchial exudates and sputa. the identification was done by biochemical test id 32 ...200718062650
biological activity of phenylpropionic acid isolated from a terrestrial streptomycetes.the strain anu 6277 was isolated from laterite soil and identified as streptomyces sp. closely related to streptomyces albidoflavus cluster by 16s rrna analysis. the cultural, morphological and physiological characters of the strain were recorded. the strain exhibited resistance to chloramphenicol, penicillin and streptomycin. it had the ability to produce enzymes such as amylase and chitinase. a bioactive compound was isolated from the strain at stationary phase of culture and identified as 3-p ...200718062653
an iron-regulated outer-membrane protein of proteus mirabilis is a haem receptor that plays an important role in urinary tract infection and in in vivo growth.proteus mirabilis, a common cause of urinary tract infections, expresses iron-regulated outer-membrane proteins (omps) in response to iron restriction. it has been suggested that a 64 kda omp is involved in haemoprotein uptake and that this might have a role in pathogenesis. in order to confirm this hypothesis, this study generated a p. mirabilis mutant strain (p7) that did not express the 64 kda omp, by insertion of the tnphoa transposon. the nucleotide sequence of the interrupted gene revealed ...200718033826
antimicrobial resistance in 11 hospitals in puerto rico: results of an antimicrobial resistance management (arm) program.the antimicrobial resistance management program (armp) was established in 1997 at the university of florida as an ongoing project to document trends in antimicrobial susceptibility patterns in inpatient/outpatient isolates and track resistance that may occur with specific antibiotic use.200718035809
a mild form of proteus syndrome.proteus syndrome is a multisystem disorder and diagnosis has been difficult because of the variability of the syndrome's manifestations. dermatologists have an important role as cutaneous findings could provide clues for the early diagnosis of the disease. herein we report a case who had cerebriform connective tissue nevus and lipoma as the sole manifestations of the disease.200718035995
proteus syndrome: 2 case reports and a review of the literature.proteus syndrome is a rare condition that has a variable clinical presentation. the syndrome is characterized by asymmetric disproportionate enlargement of the limbs, skull, or vertebrae, associated with a number of cutaneous lesions. herein, the clinical findings of 2 patients with proteus syndrome are presented and the current clinical diagnostic criteria are reviewed.200718038691
identification and antimicrobial susceptibility of bacteria and yeasts isolated from healthy dogs and dogs with otitis externa.the bacterial and fungal flora of the external ear canal of dogs with otitis externa and of healthy dogs were studied. the most frequently isolated microorganism from otitic ears was staphylococcus intermedius (58.8%), followed by malassezia pachydermatis (30.9%), streptococcus canis (29.9%), proteus spp. (14.4%) and escherichia coli (10.3%). a statistical analysis of our results showed that the prevalence of these microorganisms is significant in dogs with otitis externa. furthermore, the antim ...200718045339
preliminary report of nad+-dependent amino acid dehydrogenase producing bacteria isolated from soil.amino acid dehydrogenases (l-amino acid: oxidoreductase deaminating; ec 1.4.1.x) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either nad+, nadp+ or fad. these enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kits to screen amino acid metabolism disorders such as phenylketonuria (pku), maple syrup urine disease ( ...200718051956
drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a regional referral hospital in uganda.wound infections have been a problem in the field of surgery for a long time. advances in control of infections have not completely eradicated this problem because of development of drug resistance. antimicrobial resistance can increase complications and costs associated with procedures and treatment.200718052868
gram-negative bacillary community acquired meningitis is not a rare entity in last two decades.the aim of this short note is to assess gram-negative bacillary community acquired meningitis (cbm) and nosocomial meningitis (nm) within 17 years nationwide survey. all cases of gram-negative bacillary cbm within 1990-2007 were assessed in national database of 372 patients with bacterial meningitis: 69 of gram-negative cases were nosocomial and 24 of gram-negative meningitis cases were cbm. those 24 cases were compared with all cbm (201 cases) for risk factors and outcome. among nosocomial gram ...200718030268
congenital hand lesion. 200718032962
brief assessment of motor function: content validity and reliability of the fine motor scale.the brief assessment of motor function fine motor scale (fms) allows rapid assessment, independent of age. this study was done to establish content validity of the fms and to demonstrate fms reliability.200718004200
developing a strategy to reduce the high morbidity of patients with long-term urinary catheters: the biomed catheter research clinic.to assess the idea of managing patients having problems with long-term catheterization (ltc, normally used when all other methods of bladder management have failed or are unsuitable) in a dedicated clinic, to present a prospective analysis of consecutive new patients attending between february 2002 and october 2006, and to establish the incidence of bladder stones in patients who have recurrent catheter encrustation and blockage.200717979927
the phakomatoses: dermatologic clues to neurologic anomalies.the phakomatoses of particular interest to neurologists including sturge-weber syndrome, neurofibromatosis type 1, neurofibromatosis type 2, bannayan-riley-ruvalcaba syndrome, and proteus syndrome are presented. the physical manifestations required for clinical diagnosis, the neurologic features, and recommendations for management are given. the molecular etiology and genetic aspects of these disorders are briefly discussed as well as future implications of on-going research.200717980311
hematozoa in endemic subspecies of common kestrel in the cape verde islands.we examined 130 common kestrel (falco tinnunculus) representing two endemic subspecies and nine resident island populations on the cape verde archipelago between 1996 and 1999 to study diversity, prevalence, and intensity of hematozoa. hematozoan diversity was very low; we detected only plasmodium fallax, a species that is rarely found in falconoformes, and, possibly, haemoproteus brachiatus. moreover, prevalence of plasmodium fallax was low (1.5%) with a mean intensity of infection of 0.05 prot ...200717984275
detection of hiv-1 sequences in intestinal bacteria of hiv/aids patients.bacterial dna isolated from the intestinal tract of 11 american and 30 slovak hiv/aids patients were analyzed by colony and dot blot hybridization assay for hiv-1 specific sequences. secondly, pcr using primers specific for the hiv-1 gag, pol and env genes for detection of hiv-1 sequences in these dna were performed.200717984947
multiple antimicrobial resistance in enterobacteriaceae isolates from pristine freshwater.a freshwater enterobacterial population (n = 111) was studied for antimicrobial and mercury resistance patterns, and for its possible association with biotic and abiotic factors in that environment. conventional biochemical tests identified klebsiella sp, morganella sp, serratia sp, escherichia sp, enterobacter sp, edwarsiella sp, proteus sp, citrobacter sp, providencia sp, and kluyvera sp. there was no correlation between antimicrobial resistance patterns of isolates and bacterial genera, but r ...200717985304
preoperative diagnosis of xanthogranulomatous pyelonephritis.the aim of this study was to evaluate the possibility of differentiating xanthogranulomatous pyelonephritis (xgpn) preoperatively from chronic pyelonephritis on the basis of demographic data, clinical parameters, and biochemical, microbiological, and radiological workups.200717987581
[integrons and their relationship with resistance phenotype in gram negative bacilli isolated in the hospital torres galdames, iquique, chile].the antimicrobial resistance is coded in genetic elements which generate a horizontal flow of information, particularly in conditions that are under strong selective pressure like the nosocomial environment. in that sense, in the last decades, gram negative bacilli have become important agents of nosocomial infection. in order to investigate the presence of integrons among clinical isolates of gram negative bacilli and their relationship with their resistance profile, we studied 88 strains isola ...200717989843
the clinical development and launch of amoxicillin/clavulanate for the treatment of a range of community-acquired infections.by the 1960s and 1970s, problems in the antibacterial treatment of infections had begun to emerge. previously active antibacterials were being compromised by the development of resistance. beta-lactamase production was identified in isolates of staphylococci, and, amongst others, in escherichia coli, proteus mirabilis, haemophilus influenzae and moraxella catarrhalis. the discovery of the potent beta-lactamase inhibitor clavulanic acid, and its protective effect on amoxicillin, a semi-synthetic ...200717997283
use of in vitro susceptibility and pathogen prevalence data to model the expected clinical success rates of tigecycline and other commonly used antimicrobials for empirical treatment of complicated skin and skin-structure infections.the overall expected coverage for tigecycline and selected antimicrobial regimens based upon prevailing susceptibility rates was evaluated for the empirical therapy of complicated skin and skin-structure infections (csssis). consecutive, non-duplicate bacterial isolates collected from 2000-2005 from patients with documented csssi in 57 medical centres located in the usa (38 centres), france (6 centres), germany (7 centres), italy (3 centres) and spain (3 centres) were used to evaluate the freque ...200717959359
newly delineated syndrome of congenital lipomatous overgrowth, vascular malformations, and epidermal nevi (clove syndrome) in seven patients.we present a series of seven patients who were previously diagnosed with proteus syndrome, but who do not meet published diagnostic criteria for this disorder and whose natural history is distinct from that of proteus syndrome. this newly recognized phenotype comprises progressive, complex, and mixed truncal vascular malformations, dysregulated adipose tissue, varying degrees of scoliosis, and enlarged bony structures without progressive bony overgrowth. we have named this condition congenital l ...200717963221
species interactions in mixed-community crystalline biofilms on urinary catheters.previous experimental investigations of the crystalline biofilms that colonize and block urinary catheters have focussed on their formation by pure cultures of proteus mirabilis. in the urine of patients undergoing long-term catheterization, p. mirabilis is commonly found in mixed communities with other urinary tract pathogens. little is known about the effect that the other species have on the rate at which p. mirabilis encrusts catheters. in the present study, a set of data on the nature of th ...200717965358
polyamines in bacteria: pleiotropic effects yet specific mechanisms.extensive data in a wide range of organisms point to the importance of polyamine homeostasis for growth. the two most common polyamines found in bacteria are putrescine and spermidine. the investigation of polyamine function in bacteria has revealed that they are involved in a number of functions other than growth, which include incorporation into the cell wall and biosynthesis of siderophores. they are also important in acid resistance and can act as a free radical ion scavenger. more recently ...200717966408
[epidemiology and treatment of acute prostatitis after prostatic biopsy].acute prostatitis is the main complication of prostatic biopsies (pb) and sometimes requires hospitalisation and appropriate antibiotic therapy. this study evaluated the pathogens responsible and proposes a statistically adapted empirical antibiotic therapy.200717969797
acquired ampc type beta-lactamases: an emerging problem in italian long-term care and rehabilitation facilities.we report the multiple detection of proteus mirabilis isolates, from 4 different long-term care and rehabilitation facilities (ltcrfs) of northern italy, resistant to expanded-spectrum cephalosporins and cephamycins and producing an acquired ampc-like beta-lactamase, named cmy-16. genotyping by pfge showed that isolates were clonally related to each other, although not identical. in all isolates the bla(cmy16) gene was not transferable by conjugation and was found to be carried on the chromosome ...200717802913
molecular epidemiology of esbetal producing p. mirabilis strains from a long-term care and rehabilitation facility in italy.we report the detection of multidrug resistant esbetal producing proteus mirabilis isolates from a long-term care and rehabilitation facility (ltcrf) in northern italy. 53% of the collected p. mirabilis strains were esbetal producers. pcr and sequencing techniques confirmed the presence of the bla(tem-92) and bla(cmy-16) resistance genes in 23/26 (88.5%) and 3/26 (11.5%) of the esbetal producers respectively. pfge showed that the tem-92 beta-lactamase producing isolates were not clonally related ...200717802927
polymerase chain reaction for the rapid diagnosis of tuberculous meningitis.polymerase chain reaction (pcr) based on the amplification of a 169 bp dna fragment specific for the mycobacterium tuberculosis complex was evaluated for the rapid diagnosis of tuberculous meningitis (tbm). a total of 105 csf specimens from clinically suspected cases of tbm were studied. clinical details of the cases and cytochemical parameters of the csf specimens were recorded. for pcr 10 csf specimens from cases other than tbm, 4 non-mycobacterial culture isolates (one strain of e coli, one s ...200717802972
in vitro antimicrobial activity of a novel propolis formulation (actichelated propolis).this study compared in vitro activities of actichelated propolis (a multicomposite material obtained with mechano-chemichal activation) and of a hydroalcoholic extract of propolis.200717953601
an unusual case of a ureteric hernia into the sciatic foramen causing urinary sepsis: successfully treated laparoscopically.we present the rare case of a ureteric hernia into the sciatic foramen. the presentation was that of a 59-year-old woman with urinary tract sepsis associated with an acute deterioration of renal function. the hernia was successfully repaired laparoscopically.200717958996
structure of a glucosyl phosphate-containing o-polysaccharide of proteus vulgaris o42.an o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of proteus vulgaris o42 and studied by sugar and methylation analyses along with 1h, 13c and 31p nmr spectroscopy. the following structure of the polysaccharide having a linear pentasaccharide phosphate repeating unit was established: -->3)-alpha-l-fucpnac4ac-(1-->4)-alpha-d-glcp-1-p-(o-->4)-alpha-d-glcpnac-(1-->3)-alpha-l-fucpnac4ac-(1-->3))-alpha-d-glcpnac6ac-(1--> where the degree of o-acetylation is approxima ...200717936254
antimicrobial susceptibility of klebsiella spp. and proteus spp. from various organ systems of horses, dogs and cats as determined in the bft-germvet monitoring program 2004-2006.a total of 120 isolates of klebsiella spp. and proteus spp. collected from horses and small animals (dogs and cats) were screened for their susceptibility to 24 different antimicrobial agents. klebsiella spp. were included from infections of the genital tract (gt) of horses (36 isolates) and the urinary/genital tract (ugt) from dogs and cats (17 isolates), while proteus spp. were from small animal (dogs and cats) infections of the ugt (37 strains) and the skin (incl. ear/mouth) (30 isolates). in ...200717939455
proteus syndrome in the aetiology of carpal tunnel syndrome. 200717939492
anti-amebic antibody activity in patients, determined with antigens prepared from virulent parasites (indirect hemagglutination assay and enzyme-linked immunosorbent assay).the serology of amebiasis is affected by low sensitivity and specificity.200717939629
effect of natural preservatives on the growth of histamine producing bacteria.present study deals with the hampering of the growth of histamine producing bacteria (hpb), by using nacl and spices which are easily available and cheaper cost wise. for this experiment, four strains of hpb viz. vibrio parahaemolyticus, bacillus cereus, pseudomonas aeruginosa and proteus mirabilis were tested against 1 to 10% concentrations of nacl and 1 to 5% concentrations of natural preservatives (turmeric, ginger and garlic) in a basal medium. hpb showed different growth rates at different ...200717915763
cowden syndrome.cowden syndrome (cs), due to germline mutations of the pten tumor-suppressor gene, is an often overlooked cancer predisposition syndrome associated with an increased risk of breast, thyroid, and endometrial cancers, as well as benign manifestations. germline pten mutations also are associated with syndromes that have not been historically connected to an increase in risk for malignancy. these disorders include bannayan-riley-ruvalcaba syndrome (brrs), proteus syndrome (ps), and proteus-like synd ...200717920899
bacterial contaminations of informally marketed raw milk in ghana.summary200717925843
brain abscess in infants and children: a retrospective study of 107 patients in northeast thailand.to review the clinical manifestation, predisposing factor location of abscess, causative organism, treatment, and outcome of brain abscess.200717926990
[phosphatase activity in amoeba proteus at ph 9.0].in the free-living amoeba amoeba proteus (strain b), after paag disk-electrophoresis of the homogenate supernatant, at using 1-naphthyl phosphate as a substrate and ph 9.0, three forms of phosphatase activity were revealed; they were arbitrarily called "fast", "intermediate", and "slow" phosphatases. the fast phosphatase has been established to be a fraction of lysosomal acid phosphatase that preserves some low activity at alkaline ph. the question as to which particular class the intermediate p ...200717933343
regulatory circuit based on autogenous activation-repression: roles of c-boxes and spacer sequences in control of the pvuii restriction-modification system.type ii restriction-modification (r-m) systems comprise a restriction endonuclease (rease) and a protective methyltransferase (mtase). after r-m genes enter a new cell, mtase must appear before rease or the chromosome will be cleaved. pvuii and some other r-m systems achieve this delay by cotranscribing the rease gene with the gene for an autogenous transcription activator (the controlling or 'c' protein c.pvuii). this study reveals, through in vivo titration, that c.pvuii is not only an activat ...200717933763
bacterial defluorination of 4-fluoroglutamic acid.fluorinated amino acids are used as enzyme inhibitors, mechanistic probes and in the production of pharmacologically active peptides. because enantiomerically pure 4-fluoroglutamate is difficult to prepare, the selective degradation of the l-isomer is a potentially convenient method of obtaining d-4-fluoroglutamate from the racemate. in this paper, we describe our investigations on the degradation of 4-fluoroglutamate by bacteria. fluoride ion was detected in resting-cell cultures of a number of ...200717901951
pattern of pathogens in ear discharge of hiv-infected children in nnewi, southeast nigeria.this is a descriptive, prospective, hospital-based study designed to determine the prevalent organisms in ear infections and their antimicrobial susceptibility patterns in hiv-infected children compared to age-and sex-matched hiv sero-negative patients.200717902505
purification, crystallization, x-ray diffraction analysis and phasing of an engineered single-chain pvuii restriction endonuclease.the restriction endonuclease pvuii from proteus vulgaris has been converted from its wild-type homodimeric form into the enzymatically active single-chain variant scpvuii by tandemly joining the two subunits through the peptide linker gly-ser-gly-gly. scpvuii, which is suitable for the development of programmed restriction endonucleases for highly specific dna cleavage, was purified and crystallized. the crystals diffract to a resolution of 2.35 a and belong to space group p4(2), with unit-cell ...200717909283
septic postpartum uterine inversion.puerperal uterine inversion is an uncommon but life threatening obstetrical emergency. a 26-year-old woman, para six, was referred from a peripheral hospital seven days after delivery, with a mass protruding per vaginum. complete uterine inversion had occurred after delivery of baby and placenta. she was resuscitated and her genital infection was treated. she had a vaginal hysterectomy upon request. her postoperative recovery was uneventful. poor management of the third stage of labour is a comm ...200717909681
production of l-carnitine by secondary metabolism of bacteria.abstract: the increasing commercial demand for l-carnitine has led to a multiplication of efforts to improve its production with bacteria. the use of different cell environments, such as growing, resting, permeabilized, dried, osmotically stressed, freely suspended and immobilized cells, to maintain enzymes sufficiently active for l-carnitine production is discussed in the text. the different cell states of enterobacteria, such as escherichia coli and proteus sp., which can be used to produce l- ...200717910757
antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent.the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ...200717913083
[the antibacterial activity of fluoroquinolones against gram-negative bacteria isolated from gifu prefecture (2005)].we investigated the susceptibility to 6 fluoroquinolones against 433 strains of gram-negative bacteria isolated from 6 medical facilities in gifu prefecture between january and september in 2005, determined by the agar dilution methods in according with the japan society of chemotherapy. we also investigated the correlation between the degree of resistance to fluoroquinolones and the amino acid substitutions in the quinolone resistance-determining region (qrdr). the tested clinical isolates were ...200717867488
frequency of urinary tract infection in catheterized dogs and comparison of bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with urinary tract infections.to determine frequency of urinary tract infections (utis) in catheterized dogs that had intervertebral disk disease (ivdd) or disease other than ivdd and compare bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with utis.200717867973
synthesis and antimicrobial activity of some novel nucleoside analogues of adenosine and 1,3-dideazaadenosine.a number of nucleoside analogues have been synthesized and evaluated for their antibacterial and antifungal activities against staphylococcus aureus, group d streptococcus, pseudomonas aeruginosa, proteus spp., salmonella spp., aspergillus fumigatus, penicillium marneffei, candida albicans, cryptococcus neoformans, and mucor spp. the compounds 1, 4, and 6 emerged as potent antibacterial agents with mic values of 0.75, 0.38, and 0.19 microm, respectively, against group d streptococcus. further, t ...200717890082
breast abscess bacteriologic features in the era of community-acquired methicillin-resistant staphylococcus aureus epidemics.increasing rates of community-acquired methicillin-resistant staphylococcus aureus (mrsa) infections have also affected the microbial profile of breast abscesses.200717891866
the type iii secretion system of proteus mirabilis hi4320 does not contribute to virulence in the mouse model of ascending urinary tract infection.the gram-negative enteric bacterium proteus mirabilis is a frequent cause of urinary tract infections (utis) in individuals with long-term indwelling catheters or with complicated urinary tracts. the recent release of the p. mirabilis strain hi4320 genome sequence has facilitated identification of potential virulence factors in this organism. genes appearing to encode a type iii secretion system (ttss) were found in a low gc-content pathogenicity island in the p. mirabilis chromosome. this islan ...200717893161
clinical epidemiology of ciprofloxacin-resistant proteus mirabilis isolated from urine samples of hospitalised patients.this study investigated the clinical characteristics of ciprofloxacin-resistant proteus mirabilis isolates from urine samples associated with nosocomial infection or colonisation, and identified the risk-factors for ciprofloxacin resistance. data for patients with ciprofloxacin-resistant p. mirabilis isolates (n=13) were compared with those for randomly selected patients with ciprofloxacin-susceptible p. mirabilis isolates (n=40) who were matched by temporal occurrence as control patients. the m ...200717850340
bacteriology of chronic suppurative otitis media--a multicenter study.bacterial predominance and antibiotic sensitivity have changed over time, making continuous and periodic surveillance necessary in guiding appropriate antibacterial therapy.200717851935
mannose-resistant proteus-like and p. mirabilis fimbriae have specific and additive roles in p. mirabilis urinary tract infections.proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (uti). it produces several types of fimbriae, including mannose-resistant proteus-like (mr/p) fimbriae and p. mirabilis fimbriae (pmf). previously, we determined that these fimbriae affect the ability of p. mirabilis to colonize the urinary tract. the objective of this study was to analyse the effect of the simultaneous lack of p. mirabilis mr/p and pmf fimbriae in uti pathogenesis. a double mutant ...200717854474
prevalence of hospital-acquired infection in a moroccan university hospital.infection control and hospital-acquired infection (hai) prevalence reports from developing countries are often not well established because of the lack of staff and resources. the aim of this study was to estimate the prevalence of hai in a moroccan hospital as well as to identify its predisposing factors.200717660013
first report of the emergence of ctx-m-type extended-spectrum beta-lactamases (esbls) as the predominant esbl isolated in a u.s. health care system.ctx-m-type extended-spectrum beta-lactamases (esbls) have become increasingly common worldwide, with the notable exception of the united states, where tem- and shv-type esbls have appeared to predominate. we have noted the emergence of esbls in our health care system (the university health system in san antonio, tx), especially in escherichia coli isolates, that preferentially hydrolyze cefotaxime rather than ceftazidime, suggesting the possibility of ctx-m-type enzymes. microbiology laboratory ...200717724160
a case of proteus syndrome with lateral embryonal vein and frontal intraosseous lipoma.a patient with proteus syndrome is reported with heretofore unreported features including a frontal intraosseous lipoma and a lateral embryonal leg vein (servelle) associated with a venous malformation. these associated anomalies further broaden the phenotypic variability of proteus syndrome.200717760489
is it a proteus syndrome? 200717762684
hormonal regulation of the contractile vacuole in amoeba proteus: the role of cyclic nucleotide phosphodiesterases. 200717668613
an urgent problem of aerobic gram-negative pathogen infection in complicated parapneumonic effusions or empyemas.complicated parapneumonic effusion or empyema is a troublesome disease with a high mortality. the most common involved microorganisms seem to have changed over recent decades, influenced by the introduction of new antibiotics, and the increase of immunocompromised hosts, and the elderly population. more epidemiological studies on the current bacteriology are needed to help us to empirically select adequate antibiotics.200717675765
profile of community- and hospital-acquired pneumonia cases admitted to basra general hospital, iraq.over an 18-month period 485 throat swabs and sputum samples were taken from patients admitted to basra general hospital, iraq, with a clinical diagnosis of pneumonia. most patients (94.0%) had community-acquired pneumonia; 29 (6.0%) had hospital-acquired pneumonia. patients aged < or = 15 years and males had the highest incidence of pneumonia. cases were analysed by type of pneumonia: bronchial (76.3%) versus lobar (23.7%), and primary (81.0%) versus secondary (19.0%). the most common pathogen w ...200717684843
[ecthyma gangrenosum in a patient with non-hodgkin lymphoma].ecthymagangrenosum is a rare disease of the skin that causes the localized necrosis of the skin and subcutaneous fat tissue, leading to the multiple ulcerations surrounded by local hyperaemia. the ulcerations are usually localized in groins, and perianal area. in the majority of cases ecthyma is caused by a pseudomonas aeruginosa sepsis. the disease usually appears in immunocompromized, most frequently hematological patients.200717687947
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