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a novel autotransporter of uropathogenic proteus mirabilis is both a cytotoxin and an agglutinin.one of the six predicted proteus mirabilis autotransporters (ats), orf c2341, is predicted to contain a serine protease motif and was earlier identified as an immunogenic outer membrane protein in p. mirabilis. the 3.2 kb gene encodes a 117 kda protein with a 58-amino-acid-long signal peptide, a 75-kda-long n-terminal passenger domain and a 30-kda-long c-terminal translocator. affinity-purified 110 kda at exhibited chymotrypsin-like activity and hydrolysed n-suc-ala-ala-pro-phe-pna and n-suc-ala ...200818430084
vascular malformation and hemangiomatosis syndromes: spectrum of imaging manifestations.objective: the purpose of this review is to describe the role of imaging and associated findings in the diagnosis of blue rubber bleb nevus syndrome, proteus syndrome, klippel-trénaunay syndrome, and kasabach-merritt syndrome. conclusion: blue rubber bleb nevus, proteus, klippel-trénaunay, and kasabach-merritt syndromes are a diverse group of vascular malformation and hemangiomatosis syndromes. both cutaneous and visceral vascular lesions are associated with these disorders. accurate diagnosis o ...200818430846
rapamycin treatment for a child with germline pten mutation.a 9-month-old boy with proteus syndrome and a de novo germline mutation in the tumor suppressor pten was referred to a specialist centre for management. over the first years of life, the patient developed life-threatening respiratory dysfunction and malnutrition because of progressive growth of hamartomas affecting the chest, mediastinum, abdomen and pelvis.200818431376
selective antimicrobial activity of maggots against pathogenic bacteria.maggot therapy, also known as biosurgery, is an ancient method for the healing of chronic infected wounds. although clinicians have reported on the beneficial activities of the lucilia sericata larvae that have been used for healing chronic wounds, the selectivity of this therapy against the different pathogenic micro-organisms that are found in chronic wounds has never been analysed. in the present study, we have investigated the in vitro activities of larval excreta/secreta both against select ...200818436596
in vitro interaction between mecillinam and piperacillin-tazobactam in the presence of azithromycin against members of the enterobacteriaceae family and pseudomonas aeruginosa.mecillinam was tested in vitro alone or in combination with piperacillin-tazobactam and azithromycin against representative species of the enterobacteriaceae family and pseudomonas aeruginosa to extend its antibacterial spectrum, and to protect mecillinam from inactivating enzymes taking advantage of the presence of tazobactam. drug interactions were studied by microdilution method, by selection of spontaneous resistant mutants on agar plates containing the drugs in combination and by time kill ...200818437840
soft tissue infections with arcanobacterium haemolyticum: report of three cases.we report here three polymicrobial wound infections associated with arcanobacterium haemolyticum in rural patients aged between 60-65 years. in two patients, one with cellulitis and the other with postoperative wound infection following amputation of the limb, arcanobacterium haemolyticum was isolated repeatedly along with beta haemolytic streptococci (bhs). the bhs belonged to lancefield's group g and group c respectively. in another patient, who was a diabetic with chronic osteomyelitis, arcan ...200818445965
secondary chromosomal attachment site and tandem integration of the mobilizable salmonella genomic island 1.the salmonella genomic island 1 is an integrative mobilizable element (ime) originally identified in epidemic multidrug-resistant salmonella enterica serovar typhimurium (s. typhimurium) dt104. sgi1 contains a complex integron, which confers various multidrug resistance phenotypes due to its genetic plasticity. previous studies have shown that sgi1 integrates site-specifically into the s. enterica, escherichia coli, or proteus mirabilis chromosome at the 3' end of thdf gene (attb site).200818446190
[pleural empyema secondary to pyonephrosis]. 200818448021
identification of a novel storage glycine-rich peptide from guava (psidium guajava) seeds with activity against gram-negative bacteria.bacterial pathogens cause an expressive negative impact worldwide on human health, with ever increasing treatment costs. a significant rise in resistance to commercial antibiotics has been observed in pathogenic bacteria responsible for urinary and gastro-intestinal infections. towards the development of novel approaches to control such common infections, a number of defense peptides with antibacterial activities have been characterized. in this report, the peptide pg-amp1 was isolated from guav ...200818448201
comparative study of electrokinetic potentials and binding affinity of lipopolysaccharides-chitosan complexes.electrokinetic properties of complexes of chitosan (ch) with lipopolysaccharides (lpss) from escherichia coli o55:b5, yersinia pseudotuberculosis 1b 598, and proteus vulgaris o25 (48/57) and their size distribution were investigated using zeta-potential distribution assay and quasi-elastic light scattering. the interaction of lps from different microorganisms with chitosan at the same w/w ratio of components (1:1) resulted in the formation of complexes in which the negative charge of lps was neu ...200818455288
reduced susceptibility of proteus mirabilis to triclosan.clinical isolates of proteus mirabilis causing catheter encrustation and blockage are susceptible to the biocide triclosan (mics of 0.2 mg/liter). studies with laboratory models of the bladder have demonstrated that the inflation of catheter retention balloons with triclosan solutions rather than water results in the diffusion of triclosan from the balloons into the surrounding urine and the inhibition of catheter encrustation by p. mirabilis. the aim of this study was to test whether the exposu ...200818180352
respiratory disease associated with bovine coronavirus infection in cattle herds in southern italy.four outbreaks of bovine respiratory disease (brd) associated with bovine coronavirus (bcov) infection in italian cattle herds were reported. in 3 outbreaks, brd was observed only in 2-3-month-old feedlot calves, whereas in the remaining outbreak, lactating cows, heifers, and calves were simultaneously affected. by using reverse transcription polymerase chain reaction (rt-pcr), bcov rna was detected in all outbreaks without evidence of concurrent viral pathogens (i.e., bovine respiratory syncyti ...200818182504
bacteriological analysis of meshes removed for complications after surgical management of urinary incontinence or pelvic organ prolapse.the aim of this study is to examine the role of bacterial infection in complications following surgical management of urinary incontinence and genital prolapse using meshes. there were sixteen prostheses removed. eight were monofilament polypropylene-knitted meshes, one was a silicone-coated polypropylene mesh, another was a collagen-coated polypropylene mesh, four were silicone-coated polyester meshes and two were polyester meshes. the most frequent cause for removal was symptomatic vaginal ero ...200818183341
the 5-ht3b subunit confers spontaneous channel opening and altered ligand properties of the 5-ht3 receptor.current receptor theory suggests that there is an equilibrium between the inactive (r) and active (r*) conformations of ligand-gated ion channels and g protein-coupled receptors. the actions of ligands in both receptor types could be appropriately explained by this two-state model. ligands such as agonists and antagonists affect receptor function by stabilizing one or both conformations. the 5-ht3 receptor is a member of the cys-loop ligand-gated ion channel superfamily participating in synaptic ...200818187416
[resistance to third generation cephalosporins in sfax hospitals, tunisia (1999-2005)].the authors wanted to assess the prevalence and to monitor the trends of resistance to broad-spectrum cephalosporins among various species of enterobacteria in the region of sfax (tunisia).200818191516
intracellular microrheology of motile amoeba proteus.the motility of amoeba proteus was examined using the technique of passive particle tracking microrheology, with the aid of newly developed particle tracking software, a fast digital camera, and an optical microscope. we tracked large numbers of endogeneous particles in the amoebae, which displayed subdiffusive motion at short timescales, corresponding to thermal motion in a viscoelastic medium, and superdiffusive motion at long timescales due to the convection of the cytoplasm. subdiffusive mot ...200818192370
characterization of a novel gene, wosa, regulating flhdc expression in proteus mirabilis.in this study, we describe wosa, a proteus mirabilis gene identified by its ability to increase swarming motility when overexpressed. at various times during the swarming cycle, the increased expression of wosa resulted in a 4- to 16-fold upregulation of the transcription of flhdc, encoding the master regulator of the flagellar cascade. in turn, the expression of flaa, encoding flagellin, was substantially increased in wosa-overexpressing strains. the overexpression of wosa also resulted in cons ...200818192389
antimicrobial potential of a lipopeptide biosurfactant derived from a marine bacillus circulans.to isolate the biologically active fraction of the lipopeptide biosurfactant produced by a marine bacillus circulans and study its antimicrobial potentials.200818194244
the high-affinity phosphate transporter pst is a virulence factor for proteus mirabilis during complicated urinary tract infection.proteus mirabilis is a ubiquitous bacterium associated with complicated urinary tract infection (uti). mutagenesis studies of the wild-type strain hi4320 in the cba mouse model of ascending utis have identified attenuated mutants with transposon insertions in genes encoding the high-affinity phosphate transporter pst (psts, psta). the transcription of the pst operon (pstscab-phou) and other members of the phosphate regulon of escherichia coli, including alkaline phosphatase (ap), are regulated b ...200818194341
proteus syndrome associated with hemimegalencephaly and ohtahara syndrome: report of two cases.the authors report two cases of brazilian children with most of the common syndromic features of proteus syndrome, such as asymmetric overgrowth of tissues, skin abnormalities, hypotonia and mental retardation. in both patients, a refractory epilepsy, compatible with ohtahara syndrome, as well as hemimegalencephaly, with asymmetric distribution of facial fat, were also diagnosed.200818082431
hydrogen and polyhydroxybutyrate producing abilities of microbes from diverse habitats by dark fermentative process.thirty five bacterial isolates from diverse environmental sources such as contaminated food, nitrogen rich soil, activated sludges from pesticide and oil refineries effluent treatment plants were found to belong to bacillus, bordetella, enterobacter, proteus, and pseudomonas sp. on the basis of 16s rrna gene sequence analysis. under dark fermentative conditions, maximum hydrogen (h(2)) yields (mol/mol of glucose added) were recorded to be 0.68 with enterobacter aerogenes egu16 followed by 0.63 w ...200818083024
experimental supratrigonal cystectomy: ii--evaluation of urinary calculi, infection, and bladder dysfunction in the pathogenesis of renal failure.the objective of this study was to evaluate the role of urolithiasis, infection, and bladder dysfunction in the pathogenesis of renal failure in rats subjected to supratrigonal cystectomy. one group of sprague-dawley rats was submitted to supratrigonal cystectomy, a second to cystectomy during which a suspension of proteus mirabilis was injected into the bladder stump, and a third to sham surgery (controls). the animals were sacrificed two months after surgery. blood pressure and serum urea and ...200818085427
isolation and purification of enterocin e-760 with broad antimicrobial activity against gram-positive and gram-negative bacteria.strain nrrl b-30745, isolated from chicken ceca and identified as enterococcus durans, enterococcus faecium, or enterococcus hirae, was initially identified as antagonistic to campylobacter jejuni. the isolate produced a 5,362-da bacteriocin (enterocin) that inhibits the growth of salmonella enterica serovar enteritidis, s. enterica serovar choleraesuis, s. enterica serovar typhimurium, s. enterica serovar gallinarum, escherichia coli o157:h7, yersinia enterocolitica, citrobacter freundii, klebs ...200818086839
antibacterial activity of plants used in traditional medicines of ghana with particular reference to mrsa.: in an ethno botanical survey carried out in the akwapim-north district of the republic of ghana, 25 plant species, used in traditional medicine to treat skin disease and/or to treat antimicrobial (viral, bacterial or protozoan) infections were identified.200818096337
antibiotic policy and prescribing strategies for therapy of extended-spectrum beta-lactamase-producing enterobacteriaceae: the role of piperacillin-tazobactam.therapy of infections caused by extended-spectrum beta-lactamase (esbl)-producing bacteria with an antimicrobial to which they are resistant results in treatment failure, higher cost and increased mortality. the clsi recommends reporting esbl-producing strains of escherichia coli, klebsiella spp. and proteus spp. as resistant to all penicillin, true cephalosporin and monobactam antimicrobials, but as susceptible to beta-lactam-beta-lactamase inhibitor combinations, including piperacillin-tazobac ...200818154544
production and characterization of a bioflocculant by proteus mirabilis tj-1.a bioflocculant tj-f1 with high flocculating activity, produced by strain tj-1 from a mixed activated sludge, was investigated with regard to its production and characterization. by 16s rdna sequence and biochemical and physiological characteristics, strain tj-1 was identified as proteus mirabilis. the most preferred carbon source, nitrogen source and c/n ratio (w/w) for strain tj-1 to produce the bioflocculant were found to be glucose, peptone and 10, respectively. tj-f1 production could be gre ...200818155905
etiology of foot intertrigo in the district of afyonkarahisar, turkey: a bacteriologic and mycologic study.foot intertrigo, occurring in the interdigital space, is mostly caused initially by dermatophytes and yeasts and less frequently by gram-negative and gram-positive bacteria. with time, a "complex" may develop in the setting of moisture and maceration that contains multiple fungal and bacterial organisms.200818202333
complicated catheter-associated urinary tract infections due to escherichia coli and proteus mirabilis.catheter-associated urinary tract infections (cautis) represent the most common type of nosocomial infection and are a major health concern due to the complications and frequent recurrence. these infections are often caused by escherichia coli and proteus mirabilis. gram-negative bacterial species that cause cautis express a number of virulence factors associated with adhesion, motility, biofilm formation, immunoavoidance, and nutrient acquisition as well as factors that cause damage to the host ...200818202436
histopathological features of proteus syndrome.proteus syndrome is a rare, sporadic overgrowth disorder for which the underlying genetic defect remains unknown. although the clinical course is well-described there is no systematic histopathological description of the lesional pathology.200818205855
evaluation of different laboratory tests for the detection of metallo-beta-lactamase production in enterobacteriaceae.clinical isolates of klebsiella pneumoniae (91), escherichia coli (49), enterobacter spp. (27), proteus mirabilis (17), citrobacter freundii (2), providencia stuartii (3) and serratia spp. (5), with various mics of imipenem, were examined for production of metallo-beta-lactamases (mbls) with different phenotypic laboratory tests that have been previously published to detect mbls in pseudomonas aeruginosa and acinetobacter spp.200818222954
synergistic antibacterial activity between thymus vulgaris and pimpinella anisum essential oils and methanol extracts.essential oils (eos) and methanol extracts obtained from aerial parts of thymus vulgaris and pimpinella anisum seeds were evaluated for their single and combined antibacterial activities against nine gram-positive and gram-negative pathogenic bacteria: staphylococcus aureus, bacillus cereus, escherichia coli, proteus vulgaris, proteus mirabilis, salmonella typhi, salmonella typhimurium, klebsiella pneumoniae and pseudomonas aeruginosa. the essential oils and methanol extracts revealed promising ...200818226481
composite active site of chondroitin lyase abc accepting both epimers of uronic acid.enzymes have evolved as catalysts with high degrees of stereospecificity. when both enantiomers are biologically important, enzymes with two different folds usually catalyze reactions with the individual enantiomers. in rare cases a single enzyme can process both enantiomers efficiently, but no molecular basis for such catalysis has been established. the family of bacterial chondroitin lyases abc comprises such enzymes. they can degrade both chondroitin sulfate (cs) and dermatan sulfate (ds) gly ...200818227125
evaluation of a modified double-disc synergy test for detection of extended spectrum beta-lactamases in ampc beta-lactamase-producing proteus mirabilis.the detection of extended-spectrum beta-lactamases (esbls) in gram-negative bacteria that produce ampc beta-lactamases is problematic. in the present study, the performance of modified double-disc synergy test (mddst) that employs a combination of cefepime and piperacillin-tazobactam for the detection of proteus mirabilis producing extended spectrum and ampc beta-lactamases was evaluated and compared with double-disc synergy test (ddst) and nccls phenotypic disc confirmatory test (nccls-pdct). a ...200818227600
[an improved model of a catheterised human bladder for screening bactericidal agents].catheter encrustation and associated blockage by a crystalline proteus mirabilis biofilm constitute a continuous problem in long-term catheterised patients. the objective of the present work was to verify a new, physiological bladder model possessing the ability to show that triclosan-blocking solutions exert bactericidal and bacteriostatic activities.200818228188
efficacy of ertapenem for consolidation therapy of extended-spectrum beta-lactamase-producing gram-negative infections: a case series report.infections caused by extended-spectrum beta-lactamase (esbl)-producing gram-negative organisms are becoming increasingly common and present significant challenges in terms of treatment. carbapenems is the antibiotic class of choice for treatment of these types of infections. ertapenem is the newest carbapenem, capable of being dosed once daily, and has some in vitro but little in vivo evidence supporting its use for the treatment of these resistant infections.200818230703
diabetic foot osteomyelitis.bone infection in the diabetic foot is always a complication of a preexisting infected foot wound. prevalence can be as high as 66%. diagnosis can be suspected in two mains conditions: no healing (or no depth decrease) in spite of appropriate care and off-loading, and/or a visible or palpated bone with a metal probe. the first recommended diagnostic step is to perform (and if necessary to repeat) plain radiographs. after a four-week treatment period, if plain radiographs are still normal, suspic ...200818242114
antibacterial and antifungal activities of different parts of tribulus terrestris l. growing in iraq.antimicrobial activity of organic and aqueous extracts from fruits, leaves and roots of tribulus terrestris l., an iraqi medicinal plant used as urinary anti-infective in folk medicine, was examined against 11 species of pathogenic and non-pathogenic microorganisms: staphylococcus aureus, bacillus subtilis, bacillus cereus, corynebacterium diphtheriae, escherichia coli, proteus vulgaris, serratia marcescens, salmonella typhimurium, klebsiella pneumoniae, pseudomonas aeruginosa and candida albica ...200818257138
evaluation of pyelonephritis in culled indoor and outdoor high parity sows.the present trial was conducted in hungary in neighboring large indoor and outdoor pig production units, belonging to the same breeding company. rejected kidneys from 201 (out of 241; 83.4%) outdoor, and 191 (out of 512, 37.3%) indoor high parity sows, with previous history of recidiving postparturient fever and excessive postparturient vulvovaginal discharge were gross pathologically bacteriologically, and histologically evaluated. all rejected kidneys revealed chronic pyelonephritis. in outdoo ...200818265757
a rare case of proteus syndrome.we report a rare case of proteus syndrome which presented with dysphagia due to unilateral tonsillar hypertrophy.200818279570
isolated plantar collagenoma not associated with proteus syndrome.isolated plantar cerebriform collagenomas are a relatively rare type of connective tissue nevus. they have been suggested to be pathognomonic of proteus syndrome. however, their presence is now considered to be a major criterion of proteus syndrome, but the diagnosis of proteus syndrome also requires the presence of other minor criteria. we present an unusual case of an acquired plantar cerebriform collagenoma, which is not associated with proteus syndrome. collagenomas, or connective tissue nev ...200818280350
antimicrobial activity of the crude extracts and five flavonoids from the twigs of dorstenia barteri (moraceae).the aim of this study was to evaluate the antimicrobial activity of the crude extract of the twigs of dorstenia barteri (dbt) as well as that of four of the five flavonoids isolated from this extract. gram-positive bacteria (six species), gram-negative bacteria (12 species) and fungi (four species) were used. the agar disc diffusion test was used to determine the sensitivity of the tested samples while the well micro-dilution was used to determine the minimal inhibition concentrations (mic) and ...200818280679
in vitro activities of doripenem and six comparator drugs against 423 aerobic and anaerobic bacterial isolates from infected diabetic foot wounds.against 182 anaerobe and 241 aerobe strains obtained from diabetic foot infections, doripenem was the most active carbapenem against pseudomonas aeruginosa (mic(90), 2 microg/ml), more active than imipenem against proteus mirabilis, and ertapenem was more active against escherichia coli and klebsiella spp. the mic(50) and mic(90) values were < or =0.125 microg/ml for methicillin-sensitive staphylococcus aureus and all streptococci and 0.25/1 for bacteroides fragilis.200818070958
effect of octenisept antiseptic on bioburden of neoplastic ulcers in patients with advanced cancer.to assess the effect of octenidine dihydrochloride (octenisept, schülke & mayr) on the clinical condition and bacterial flora in neoplastic ulcers.200818210953
in vitro activity profile of ceftobiprole, an anti-mrsa cephalosporin, against recent gram-positive and gram-negative isolates of european origin.to determine the in vitro activity profile of ceftobiprole, a pyrrolidinone cephalosporin with activity against methicillin-resistant staphylococcus aureus (mrsa), for use as a contemporary baseline to help detect any changes in its activity profile throughout the course of clinical development and use.200818218646
performance of chromid esbl, a chromogenic medium for detection of enterobacteriaceae producing extended-spectrum beta-lactamases.the chromogenic agar medium chromid esbl (biomérieux) was compared with blse agar medium (aes) for selective isolation and presumptive identification of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae from clinical samples. a total of 765 samples (468 rectal swabs, 255 urine samples and 42 pulmonary aspirations) obtained from 547 patients was processed. all bacterial strains isolated on either medium were further characterized using biochemical tests, and esbl producers were ...200818287293
isolation, identification and application of novel bacterial consortium tj-1 for the decolourization of structurally different azo dyes.a novel bacterial consortium (tj-1), which could decolorize acid orange 7 (ao7) and manyother azo dyes, was developed. in tj-1 three bacterial strains were identified as aeromonas caviae, proteus mirabilis and rhodococcus globerulus by 16s rrna gene sequence analysis. ao7 decolorization was significantly higher with the use of consortium as compared to the use of individual strains, indicating complementary interactions among these strains. ao7 decolorization was observed under microaerophilic c ...200818289845
increase in beta-lactam-resistant proteus mirabilis strains due to ctx-m- and cmy-type as well as new veb- and inhibitor-resistant tem-type beta-lactamases.the aim of this study was to characterize the different inhibitor-resistant tem beta-lactamases, extended-spectrum beta-lactamases (esbls) and plasmid-mediated ampc beta-lactamases implicated in beta-lactam resistance in proteus mirabilis, which has increased over recent years.200818292096
surveillance for plasmid-mediated quinolone resistance determinants in enterobacteriaceae within the calgary health region, canada: the emergence of aac(6')-ib-cr.a study was designed to determine the prevalence of plasmid-mediated quinolone resistance (pmqr) determinants among clinical isolates of enterobacteriaceae from the calgary health region (chr).200818296438
viper metalloproteinase (agkistrodon halys pallas) with antimicrobial activity against multi-drug resistant human pathogens.metalloproteinases are abundant enzymes in crotalidae and viperidae snake venoms. snake venom metalloproteinases (svmps) comprise a family of zinc-dependent enzymes, which display many different biological activities. a 23.1 kda protein was isolated from agkistrodon halys (pallas, chinese viper) snake venom. the toxin is a single chain polypeptide with a molecular weight of 23146.61 and an n-terminal sequence (miqvllvticlavfpyqgssiiles) relatively similar to that of other metalloprotein-like pro ...200818297685
synthesis, structure and biological activities of cobalt(ii) and zinc(ii) coordination compounds with 2-benzimidazole derivatives.in this work we present the synthesis, structural and spectroscopic characterisation of a series of cobalt(ii) and zinc(ii) coordination compounds with benzimidazole (bz) and its 2-benzimidazole derivatives: 2-aminobenzimidazole (2ab), albendazole (abz) and tris(2-benzimidazolylmethyl)amine (ntb). the compounds were evaluated for their in vitro antimicrobial activity against staphylococcus aureus, micrococcus luteus, salmonella typhi, pseudomonas aeruginosa, escherichia coli and proteus vulgaris ...200818304644
case 131: proteus syndrome. 200818309020
experimental and qsar studies on antimicrobial activity of benzimidazole derivatives.twenty eight analogues of benzimidazoles had been synthesized and tested for their antimicrobial activity against four bacteria (staphylococcus auerus, escherichia coli, bacillus pumilus and proteus vulgaris) and two fungi (aspergillus flavus and aspergilus niger). compounds with r as c6h4no2 and r' as so2c6h4-ch3(p), with r as c6h4och3 and r' as so2c6h4-ch3(p), and with r as ch2c6h5 and r' as ch2(ch2)9cl exhibited comparable or higher antibacterial activity than ciprofloxacin against s. auerus ...200818310935
identification of an inhibitor of the murc enzyme, which catalyzes an essential step in the peptidoglycan precursor synthesis pathway.the pathway for synthesis of the peptidoglycan precursor udp-n-acetylmuramyl pentapeptide is essential in gram-positive and gram-negative bacteria. this pathway has been exploited in the recent past to identify potential new antibiotics as inhibitors of one or more of the mur enzymes. in the present study, a high-throughput screen was employed to identify potential inhibitors of the escherichia coli murc (udp-n-acetylmuramic acid:l-alanine ligase), the first of four paralogous amino acid-adding ...200818315498
role of rppa in the regulation of polymyxin b susceptibility, swarming, and virulence factor expression in proteus mirabilis.proteus mirabilis, a human pathogen that frequently causes urinary tract infections, is intrinsically highly resistant to cationic antimicrobial peptides, such as polymyxin b (pb). to explore the mechanisms underlying p. mirabilis resistance to pb, a mutant which displayed increased (> 160-fold) sensitivity to pb was identified by transposon mutagenesis. this mutant was found to have tn5 inserted into a novel gene, rppa. sequence analysis indicated that rppa may encode a response regulator of th ...200818316383
microbial keratitis in a case of lamellar ichthyosis.we report a case of microbial keratitis in a patient with lamellar ichthyosis. an 11-year-old boy, a known case of lamellar ichthyosis, presented with microbial keratitis. microbiological evaluation of corneal scraping revealed a mixed infection caused by gram-negative bacilli and gram-positive cocci. he was treated with topical ciprofloxacin and fortified cefazolin eyedrops. microbial keratitis in patients with lamellar ichthyosis has a poor prognosis.200817828596
endoscopic treatment of pituitary abscess: two case reports and literature review.pituitary abscess is a rare entity and the correct diagnosis is still difficult before surgery. more than 210 cases have been reported in the medical literature, mostly in the form of isolated case reports. we report two cases of pituitary abscess treated endoscopically and we review the literature. a 30-year-old woman and a 35-year-old man were admitted with a history of pituitary dysfunction. patient 1 presented with polyuria, polydipsia, amenorrhea, headache, and visual impairment. magnetic r ...200817899232
prevalence of extended-spectrum beta-lactamases in proteus mirabilis in a taiwanese university hospital, 1999 to 2005: identification of a novel ctx-m enzyme (ctx-m-66).a total of 1574 nonduplicate proteus mirabilis isolates collected at a taiwanese hospital during 1999 to 2005 were analyzed for production of extended-spectrum beta-lactamases (esbls). forty-four esbl-producing isolates including 22 ctx-m-14, 18 ctx-m-3, 2 ctx-m-24, and 2 ctx-m-66 producers were detected, and the proportion of esbl producers increased from 0.7% in 1999 to approximately 6% after 2002. ctx-m-66 is a novel variant of ctx-m esbls that differs from ctx-m-3 by a ser to asn change at a ...200817913434
structure and mode of action of the antimicrobial peptide arenicin.the solution structure and the mode of action of arenicin isoform 1, an antimicrobial peptide with a unique 18-residue loop structure, from the lugworm arenicola marina were elucidated here. arenicin folds into a two-stranded antiparallel beta-sheet. it exhibits high antibacterial activity at 37 and 4 degrees c against gram-negative bacteria, including polymyxin b-resistant proteus mirabilis. bacterial killing occurs within minutes and is accompanied by membrane permeabilization, membrane detach ...200817935487
synthesis, characterization and antibacterial activity of cobalt(iii) complexes with pyridine-amide ligands.the ligands 2-(n-(x-pyridyl)carbamoyl)pyridine (x=2, 3 or 4 for hl1-hl3, respectively) and 2,6-bis(n-(y-pyridyl)carbamoyl)pyridine (y=2, 3 or 4 for h2l4-h2l6, respectively) in their mono- and di-deprotonated forms have been used to synthesize kinetically stable cobalt(iii) compounds [co(l1-3)3] (1-3) and na[co(l4-6)2] (4-6), respectively. the co(iii) ion is in octahedral environment and is surrounded by three bidentate ligands in complexes 1-3 and two tridentate ligands in complexes 4-6. ligands ...200817959275
rheumatoid arthritis in smokers could be linked to proteus urinary tract infections.there is an interplay between genetic and environmental factors in the induction and development of rheumatoid arthritis (ra). the ra-associated hla-drb1 alleles which contain the shared epitope moiety as well as microbial triggers such as proteus are involved in the aetiopathogenesis of this disease. increased association between proteus urinary tract infections (utis) and ra on one hand and the link between smoking and utis on the other hand could explain the increased frequency of ra among sm ...200817983708
type 2 segmental cowden disease vs. proteus syndrome: reply from authors. 200817999697
presence of aquaporin and v-atpase on the contractile vacuole of amoeba proteus.the results of water permeability measurements suggest the presence of an aqp (aquaporin) in the membrane of the cv (contractile vacuole) in amoeba proteus [nishihara, shimmen and sonobe (2004) cell struct. funct. 29, 85-90].200818004980
rmtc 16s rrna methyltransferase in australia. 200818025117
salmonella genomic island 1 (sgi1), variant sgi1-i, and new variant sgi1-o in proteus mirabilis clinical and food isolates from china.salmonella genomic island 1 (sgi1) and variants (sgi1-i and the new variant sgi1-o) were mapped in five strains of proteus mirabilis isolated from humans and food in china. sequencing showed that sgi1 and variants were integrated at the 3' end of the chromosomal thdf gene as previously described for salmonella strains.200818025121
in vitro activities of tigecycline combined with other antimicrobials against multiresistant gram-positive and gram-negative pathogens.to test the activity of tigecycline combined with 16 antimicrobials in vitro against 22 gram-positive and 55 gram-negative clinical isolates.200818033780
antimicrobial activities of the bromophenols from the red alga odonthalia corymbifera and some synthetic derivatives.a series of bromophenols was obtained by isolation from red alga odonthalia corymbifera and by reactions of bis(hydroxyphenyl)methanes with bromine. new bromophenols including 3,3',5,5'-tetrabromo-2,2',4,4'-tetrahydroxydiphenylmethane (10), a regioisomer of the potent antimicrobial natural product, together with known derivatives were synthesized in high yield. all of the isolated and synthesized compounds were tested for antimicrobial activity against gram-negative, gram-positive bacteria and f ...200818053715
detection of plasmid-mediated ampc in escherichia coli, klebsiella pneumoniae and proteus mirabilis.this study investigated the prevalence of plasmid-mediated ampc production in selected clinical isolates of escherichia coli, klebsiella species and proteus mirabilis, and compared the results of boronic acid disc screening with conventional susceptibility testing for the detection of ampc-positive isolates.200818057079
activities of the frog skin peptide, ascaphin-8 and its lysine-substituted analogs against clinical isolates of extended-spectrum beta-lactamase (esbl) producing bacteria.extended-spectrum beta-lactamase (esbl)-producing gram-negative bacteria are becoming increasingly prevalent and their antibiotic resistance necessitates novel therapeutic intervention. ascaphin-8 is a cationic alpha-helical peptide that shows broad-spectrum antibacterial activity but is also toxic to human erythrocytes (lc(50)= 55 microm). this study assesses the activity of ascaphin-8, and a series of l-lysine-substituted analogs, against a range of clinical isolates of esbl-producing bacteria ...200818068868
new triazole and triazolothiadiazine derivatives as possible antimicrobial agents.triazole and triazoles fused with six-membered ring systems are found to possess diverse applications in the fields of medicine, agriculture and industry. the new 1,2,4-triazole and 1,2,4-triazolo[3,4-b][1,3,4]thiadiazine derivatives were synthesized as novel antimicrobial agents. the reaction of 1h-indol-3-acetic acid with thiocarbohydrazide gave the 4-amino-3-mercapto-5-[(1h-indol-3-yl)methyl]-4h-1,2,4-triazole. the reaction of triazole with arylaldehydes in ethanol gave the 4-arylideneamino-3 ...200817499887
diverse tetracycline resistant bacteria and resistance genes from coastal waters of jiaozhou bay.environmental microbiology investigation was carried out in jiaozhou bay to determine the source and distribution of tetracycline-resistant bacteria and their resistance mechanisms. at least 25 species or the equivalent molecular phylogenetic taxa in 16 genera of resistant bacteria could be identified based on 16s ribosomal deoxyribonucleic acid sequence analysis. enterobacteriaceae, pseudomonadaceae, and vibrionaceae constituted the majority of the typical resistant isolates. indigenous estuari ...200817668262
antimicrobial activity of essential oils of ocimum gratissimum l. from different populations of kenya.hydro-distilled volatile oils from the leaves of ocimum gratissimum l. (lamiaceae) of 13 populations of different silvicultural zones were evaluated for antimicrobial activity against gram positive (staphylococcus aereus, bacillus spp.) and gram negative (escherichia coli, pseudomonas aeruginosa, samonella typhi, klebisiella pneumoniae, proteus mirabilis) bacteria and a pathogenic fungus, candida albicans. all the essential oils are active to the tested microbiles with different strength. the hi ...200820161936
evaluation of phytochemical and antibacterial potential of helicteres isora l. fruits against enteric bacterial pathogens.antibacterial activities of aqueous, acetone, ethanol and methanol extracts of fruits of helicteres isora (mororphali) were studied. the fruit aqueous extracts of h. isora showed prominent antibacterial activities against e. coli, staphylococcus epidermidis, salmonella typhimurium and proteus vulgaris; moderate activity against enterobacter aerogenes, staphylococcus aureus, salmonella typhi and least activity against pseudomonas aeruginosa. the aqueous extract showed maximal, the ethanol and met ...200820161950
isolation of bacteria causing secondary bacterial infection in the lesions of cutaneous leishmaniasis.cutaneous leishmaniasis (cl) is a parasitic disease characterized by single or multiple ulcerations. secondary bacterial infection is one of the complications of the disease that can increase the tissue destruction and the resulting scar.200819882011
frequency of renal stone disease in patients with urinary tract infection.to determine incidence of renal stone disease in patients with urinary tract infection, this cross sectional study was conducted at surgical unit of district head quarters hospital, charsadda over a period of one year from january to december 2005.200819024188
antibacterial activity of acalypha indica l.hexane, chloroform, ethyl acetate and methanol extracts from the leaves of acalypha indica were tested against gram-positive (staphylococcus aureus, staphylococcus epidermidis, bacillus cereus, streptococcus faecalis) and gram-negative (klebsiella pneumoniae, escherichia coli, proteus vulgaris, pseudomonas aeruginosa) bacteria. all the extracts exhibited antibacterial activity against gram-positive organisms with minimum inhibitory concentrations (mic) between 0.156 to 2.5 mg/ml. among the gram- ...200819024213
prevalence and characterization of verotocytoxin producing escherichia coli o157 from diarrhoea patients in morogoro, tanzania.escherichia coli o157:h7 is an important agent of haemorrhagic colitis and haemolytic uraemic syndrome in children less than five years old and elderly people. the objective of this study was to investigate the prevalence of verotocytoxin producing e. coli 0157 (vtec o157) among human patients with diarrhoea in morogoro, tanzania. faecal samples originating from 275 human patients with diarrhoea were screened for presence of e. coli o157:h7. a total of 96 e. coli isolate were identified. of thes ...200819024340
[nationwide susceptibility surveillance of ciprofloxacin and various parenteral antimicrobials against bacteria isolated from patients with severe infections--third ciproxan injection special survey (2005)].we conducted 3 nationwide surveillance studies between 2001 and 2005 at 39 participating institutions throughout japan according to the special survey plan to investigate susceptibility to ciprofloxacin (cpfx) and various parenteral antimicrobials using clinical isolates from patients with severe infection during the reexamination period of parenteral cpfx. results of the first special survey (2001) were already reported in this journal. the current third special survey (2005) was conducted at 3 ...200819024645
nationwide surveillance in taiwan of the in-vitro activity of tigecycline against clinical isolates of extended-spectrum beta-lactamase-producing enterobacteriaceae.tigecycline in-vitro surveillance in taiwan (tist), initiated in 2006, is a nationwide surveillance programme designed to monitor longitudinally the in-vitro activity of tigecycline against commonly encountered resistant bacteria. this study compared the in-vitro activity of tigecycline against clinical isolates of resistant gram-negative bacteria determined by the broth microdilution and etest methods. a total of 622 isolates were collected from patients treated at 20 teaching hospitals. tigecy ...200819013351
incomplete renal tubular acidosis as a predisposing factor for calcium phosphate stones in neuropathic bladder: a case report.abstract: we present a male tetraplegic patient, who developed stones in neuropathic bladder six times within a span of three years. unusual features of this case are: (1) this patient started developing stones in urinary bladder thirteen years after sustaining spinal cord injury. (2) he was performing intermittent catheterisation and did not have an indwelling catheter. (3) the presenting symptom of vesical lithiasis was abdominal spasms and not urine infection. (4) the major component of the s ...200819014688
susceptibility pattern of some clinical bacterial isolates to selected antibiotics and disinfectants.the antibacterial activities of five antibiotics, three brands of ofloxacins (obenasin, floxavid and drovid) and two brands of ciprofloxacins (uroxin and siprosan), and five commonly used disinfectants (lysol, dettol, purit, roberts and wex-cide) against staphylococcus aureus, escherichia coli, proteus spp., pseudomonas aeruginosa, streptococcus spp. and bacillus spp. were investigated. the growth inhibitory effect of both the antibiotics and disinfectants were determined using paper disk diffus ...200819004240
bacteriology of non-surgical wound infections in ibadan, nigeria.previous studies done on wound infections in this environment had been mostly on the surgical variety rather than the non-surgical. however,few studies available on the non-surgical type have indicated that changes do occur in the pattern and antibiogram of the bacterial isolates of these non-surgical wound infections. this study was therefore designed to investigate the bacteriology of non-surgical wound infections in this environment. a retrospective review of seven hundred and fifty four case ...200818982819
ascomycones a-c, heptaketide metabolites from an unidentified ascomycete.the ascomycones a-c (1-3), three novel heptaketide-derived secondary metabolites, have been isolated from cultures of an unknown ascomycete. while 1 and 2 are closely related pyranonaphthoquinones, 3 has a bicyclic dihydronaphthoquinone core. compounds 2 and 3 exhibited significant activity against several phytopathogenic fungi.200818989977
highly resistant microorganisms in a teaching hospital: the role of horizontal spread in a setting of endemicity.to determine the incidence density of highly resistant organisms (hros) and the relative contribution of horizontal spread in a setting of endemicity.200818973456
dna adenine methylation of sams1 gene in symbiont-bearing amoeba proteus.the expression of amoeba sams genes is switched from sams1 to sams2 when amoebae are infected with legionella jeonii. to elucidate the mechanism for the inactivation of host sams1 gene by endosymbiotic bacteria, methylation states of the sams1 gene of d and xd amoebae was compared in this study. the sams1 gene of amoebae was methylated at an internal adenine residue of gatc site in symbiont-bearing xd amoebae but not in symbiont-free d amoebae, suggesting that the modification might have caused ...200818974959
in vitro study to compare sensitivity of amoxicillin+clavulanic acid and cefpodoxime+clavulanic acid among beta-lactamase positive clinical isolates of gram-positive and gram-negative pathogens.the present study was carried out to compare the in vitro sensitivity of cefpodoxime + clavulanic acid and amoxicillin + clavulanic acid against 55 gram-positive and 123 gram-negative beta-lactamase positive clinical isolates. micro-organisms isolated from different clinical specimens were tested for beta-lactamase/esbl by using nitrocefin disc test and for metallo beta-lactamase by using double disc synergy test. a total of 299 (93 gram-positive and 206 gram-negative) clinical isolates were tes ...200818975519
prevalence and beta-lactamase producing bacterial isolates in a teaching hospital in peshawar, pakistan: a four year study.prevalence of microorganisms was studies in clinical samples of various body fluids (n=12,259), collected from patients at post graduate medical institute hayatabad medical complex (pgmi, hmc) peshawar. only 34.90% of samples exhibited growth, 36.25% of these isolates were gram positive and 63.75% were gram negative bacteria. e. coli was the most prevalent organism (39.45%) followed by s. aureus (32.23%), proteus spp. (9.23%), pseudomonas spp. (6.54%), streptococuss spp. (3.51%), acinetobacter s ...200818930859
determination of pathogenic bacteria by cze with surface-modified capillaries.the importance of electromigration techniques in molecular biology and medicine is increasing rapidly, especially in systematic studies on proteomes and metabolomes. staphylococcus aureus and escherichia coli are bacterial species most frequently encountered in human infections, and many serious illnesses can be observed in the hospital environment. in this contribution we proposed a ce method with different modification of internal capillary surface and with monolithic beds as a selective mater ...200818942676
detection of protozoan and bacterial pathogens of public health importance in faeces of corvus spp. (large-billed crow).parasites and bacteria are reported in the faeces of birds in the current study. fresh faecal samples of the large-billed crow (corvus spp.) were collected from the study site at bangsar, an urban setting in kuala lumpur, malaysia. these samples were transported to laboratory and analysed for parasites and bacteria. pre-prepared xld agar plates were used for culturing the bacteria in the laboratory. using the api 20etm test strips, 9 different species of bacteria were identified belonging to the ...200818948884
purple like a glove. 200818951389
[purple urine bag syndrome]. 200818922671
bacterial biofilms in patients with indwelling urinary catheters.bacteria have a basic survival strategy: to colonize surfaces and grow as biofilm communities embedded in a gel-like polysaccharide matrix. the catheterized urinary tract provides ideal conditions for the development of enormous biofilm populations. many bacterial species colonize indwelling catheters as biofilms, inducing complications in patients' care. the most troublesome complications are the crystalline biofilms that can occlude the catheter lumen and trigger episodes of pyelonephritis and ...200818852707
a prospective study of ceftriaxone treatment in acute pyelonephritis caused by extended-spectrum beta-lactamase-producing bacteria.much controversy exists as to whether cephalosporin treatment is appropriate for infections caused by esbl-producing organisms because no randomized controlled studies have been performed.200818788687
design, synthesis, and evaluation of novel organophosphorus inhibitors of bacterial ureases.a new group of organophosphorus inhibitors of urease, p-methyl phosphinic acids was discovered by using the structure based inhibitor design approach. several derivatives of the lead compound, aminomethyl(p-methyl)phosphinic acid, were synthesized successfully. their potency was evaluated in vitro against urease from bacillus pasteurii and proteus vulgaris. the studied compounds constitute a group of competitive, reversible inhibitors of bacterial ureases. obtained thiophosphinic analogues of th ...200818717581
antibacterial activity of halogenated sesquiterpenes from malaysian laurencia spp.during our studies on malaysian laurencia species, brominated metabolites, tiomanene, acetylmajapolene b, and acetylmajapolene a were isolated from an unrecorded species collected at pulau tioman, pahang along with known majapolene b and majapolene a. acetylmajapolene a was a mixture of diastereomers as in the case of majapolene a. tiomanene may be a plausible precursor for acetylmajapolenes b and a. in addition, three known halogenated sesquiterpenes and two known halogenated c(15) acetogenins ...200818718619
prevalence and mechanisms of broad-spectrum beta-lactam resistance in enterobacteriaceae: a children's hospital experience.the objective of this study was to investigate the trends and patterns of resistance in beta-lactamase-producing members of the family enterobacteriaceae in a children's hospital over a 9-year period (1999 to 2007). clinically significant isolates of the enterobacteriaceae were screened for patterns of broad-spectrum resistance to beta-lactams. the strains likely to be resistant were subsequently confirmed by an inhibitor-based disc test. the plasmid-mediated resistance determinants in these iso ...200818765688
[lower urinary tract infections: bacterial epidemiology and recommendations].lower urinary tract infection remains frequent particularly in women, despite improvement in therapeutic means. it seems likely to revisit bacterial epidemiology and therapeutic available strategies. to analyze elements in presence i.e. infecting bacteria and antibiotics still active or identification of acquired resistance mechanisms should permit to establish the evolution during the last 10 years. the study shows that bacterial epidemiology in urinary tract infection has not changed significa ...200818773847
prevalence of quinolone-resistant urinary tract infections in comanche county memorial hospital.urinary tract infection (uti) is not only a common outpatient but also the most frequently occurring nosocomial infection. the most common causative organisms of uti remain escherichia coli, nosocomial gram-negative bacilli, enteroccoci and candida. many physicians use quinolones as the agent of choice for treatment of uti. as calculated by the drug utilization data of 2007, the rate of quinolone use by physicians for uti is 48%. this study was performed to determine the prevalence of quinolone- ...200818856050
comparison of pcr-based molecular markers for the characterization of proteus mirabilis clinical isolates.proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. for epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (pfge) or ribotyping, have been developed for this pathogen. however, these methods are labor intensive a ...200819219283
[occupational exposure to mesophilic microorganisms associated with commercial processing of compost for mushroom production].the aim of the study was to assess the occupational exposure to mesophilic microorganisms associated with commercial processing of compost for mushroom production.200819227882
antimicrobial activity of some macrophytes from lake manzalah (egypt).the antimicrobial activities of aqueous and organic solvents (chloroform, ethanol and methanol) extracts of four plants ceratophyllum demersum l., eichhornia crassipes, potamogeton crispus and potamogeton pectinatus were tested in vitro against seventeen different microorganisms including gram-positive and gram-negative bacteria and fungi. nine of these identified organisms were obtained from different sources, bacillus subtilis 1020, bacillus cereus 1080, staphylococcus aureus, erwinia carotovo ...200819205264
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