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Antimicrobial susceptibility of Gram-negative organisms from intraabdominal infections and evolution of isolates with extended spectrum ß-lactamases in the SMART study in Spain (2002-2010).Introduction. The SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study records the antimicrobial susceptibility of Gram-negative bacilli obtain from intraabdominal infections with special focus in isolates with extended spectrum ß-lactamases (ESBLs). Material and Methods. The antimicrobial susceptibility of 8,869 isolates was analyzed by microdilution during the SMART study performed in Spain from 2002 to 2010. Isolates were recovered in 16 centres. Results. Escherichi ...201122173194
Origins of the elephant man: mosaic somatic mutations cause Proteus syndrome. 201122035467
synthesis, characterization, theoretical crystal structure, and antibacterial activities of some transition metal complexes of the thiosemicarbazone (z)-2-(pyrrolidin-2-ylidene)hydrazinecarbothioamide.problem statement. in iraq like most third world countries, attempts discovered new antibiotic drugs derived from thiosemicarbazide and its metal complexes and developed the branch of applied in organic chemistry. approach. new (z)-2-(pyrrolidin-2-ylidene)hydrazinecarbothioamide (l) was synthesized in a good yield by the reaction of pyrrolidone with thiosemicarbazide. co(ii), ni(ii), and cu(ii) complexes of (l) were prepared and characterized by ft-ir, uv/visible spectra, (1)hnmr, and chn analys ...201121804771
a new class of 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazine analogues with antimicrobial/antimycobacterial activity.this study presents the synthesis and in vitro pharmacological evaluations of novel 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazines. the title compounds were assayed for their in vitro antimicrobial activity against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria) and four fungi (aspergillus niger, aspergillus fumigatus, a ...201121740100
giant chondrolipoma of the tongue in a 14-year-old child with mandibular hypertrophy. isolated lesion or regional overgrowth syndrome.chondrolipoma is a rare condition; especially in the oral cavity. the authors described a giant chondrolipoma of the tongue, associated with mandibular and lower lip overgrowth, in a 14-year-old boy. after tumour excision, histopathological examination showed mature cartilage within lobules of mature adipocytes. this is the first case of giant chondrolipoma associated with facial overgrowth. the aetiology and the association with a localized proteus syndrome are discussed.201121802258
a mosaic activating mutation in akt1 associated with the proteus syndrome.background the proteus syndrome is characterized by the overgrowth of skin, connective tissue, brain, and other tissues. it has been hypothesized that the syndrome is caused by somatic mosaicism for a mutation that is lethal in the nonmosaic state. methods we performed exome sequencing of dna from biopsy samples obtained from patients with the proteus syndrome and compared the resultant dna sequences with those of unaffected tissues obtained from the same patients. we confirmed and extended an o ...201121793738
hamartoma syndromes, exome sequencing, and a protean puzzle.gross malformations have been well known for centuries, even millennia. however, it was not until 1904 that albrecht coined the concept of hamartoma. in 1934, the concept entered the field of developmental pathology generally in reference to tissue malformations. hamartomata are localized overgrowths of a single tissue or combination of tissues, indigenous to the affected body part or organ, usually growing at the same rate as the normal components and causing little pain or functional impairmen ...201121793737
improved detection of ampc β-lactamases in escherichia coli, klebsiella pneumoniae, and proteus mirabilis using new susceptibility breakpoints for third-generation cephalosporins. 201121549542
new delhi metallo-ss-lactamase-1: detection and prevention. 201121788418
detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (sift-ms).selected ion flow tube-mass spectrometry has been used to measure the volatile compounds occurring in the headspace of urine samples inoculated with common urinary tract infection (uti)-causing microbes escherichia coli, proteus vulgaris, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, enterococcus faecalis, or candida albicans. this technique has the potential to offer rapid and simple diagnosis of the causative agent of utis.201121741416
n-substituted aminomethanephosphonic and aminomethane-p-methylphosphinic acids as inhibitors of ureases.small unextended molecules based on the diamidophosphate structure with a covalent carbon-to-phosphorus bond to improve hydrolytic stability were developed as a novel group of inhibitors to control microbial urea decomposition. applying a structure-based inhibitor design approach using available crystal structures of bacterial urease, n-substituted derivatives of aminomethylphosphonic and p-methyl-aminomethylphosphinic acids were designed and synthesized. in inhibition studies using urease from ...201121559954
[clinical and economic expedience of ertapenem therapy of complicated urinary tract infection].clinical and economic investigation of various antibiotics use in the treatment of complicated urinary tract infection (cuti) was performed under the russian economic environment. the drugs of comparison were ertapenem, ceftriaxone and levofloxacin. direct costs and their structure were shown, and the cost efficiency was calculated. alternative analysis and one-side susceptibility analysis were performed. in complicated urinary tract infections when the major pathogens were escherichia coli, kle ...201121780670
Macrodactyly with skin hypertrophy: a minimal form of the Proteus syndrome.The Proteus syndrome was described 1983 . It has asymmetric gigantism of the limbs, verrucous epidermal naevi, cerebriform enlargement of the plantar region, vascular malformations and neoplasms, as lipomas. It received this denomination after Proteus from the Greek mythology, who had the ability to change his form . A 15 year-old boy, reported a congenital hypertrophy with syndactily of the second and third right fingers . The second case is a 35 year-old man, who reported that since birth the ...201121738976
Trimethoprim/sulfametrole: evaluation of the available clinical and pharmacokinetic/pharmacodynamic evidence.Emergence of resistance to widely used trimethoprim/sulfamethoxazole (TMP/SMX) as well as common adverse events in human immunodeficiency virus (HIV)-infected patients casts interest on combinations of TMP with other sulfonamides. Sulfametrole (SMT) combined with TMP could provide a choice for difficult-to-treat infections, particularly when administered intravenously. The objective of this review was to evaluate the available clinical and pharmacokinetic/pharmacodynamic (PK/PD) evidence regardi ...201121741802
[proteus mirabilis isolated from urine, resistance to antibiotics and biofilm formation].in the rather rare urinary pathogen proteus mirabilis resistance to antibiotics and ability to form biofilm were studied.201121780025
the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds.the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ...201122182219
vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital.an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ...201121917010
embolic capture angioplasty of lower extremity lesion following distal embolization.peripheral embolization is a known complication of peripheral artery interventions. it can lead to occlusion of distal vessels leading to significant lower extremity ischemia and complications. peripheral artery interventions involving chronic total occlusions have been shown to have higher rates of complications including distal embolization. although distal embolic protection strategies are available, they are seldom utilized during lower extremity interventions, especially during treatment of ...201121616726
potentiation of antimicrobial activity of ciprofloxacin by pelargonium graveolens essential oil against selected uropathogens.the recent approach of using herbs and antibiotics in combination constitutes a strategy to overcome the problems of resistance and side effects associated with conventional antibiotics. in the present study, the antimicrobial effect of pelargonium graveolens l' hér essential oil in combination with ciprofloxacin was evaluated on uropathogens, namely, klebsiella pneumoniae kt2, proteus mirabilis prt3 and staphylococcus aureus st2. minimum inhibitory concentrations of p. graveolens essential oil ...201121618302
carbon nanoparticles-assisted mediator-less microbial fuel cells using proteus vulgaris.recently mediator-less microbial fuel cells (mfcs) are attracting great interest among researchers due to their potential applications to electricity generation as well as wastewater treatment. common mediator-less mfcs employ electroactive bacteria called exoelectrogens to directly transfer electrons to the anode from the bacteria. however, exoelectrogens are rather limited in number and thus may not find general use for practical purposes. here we showed our results in which mediator-less mfcs ...201121764289
superior in vitro activity of ertapenem and piperacillin/tazobactam against recent clinical isolates of proteus mirabilis from intra-abdominal infections (smart 2009-2010). 201121632217
Plasmid-mediated transfer of the bla(NDM-1) gene in Gram-negative rods.The latest threat of multidrug-resistant Gram-negative bacteria corresponds to the emergence of carbapenemase NDM-1 (New Delhi metallo-ß-lactamase) producers, mostly in Enterobacteriacae. Five bla(NDM) (-1) -positive plasmids of different incompatibility groups (IncL/M, FII, A/C and two untypeable plasmids) from clinical Enterobacteriaceae were evaluated for conjugation properties and host specificity. Successful conjugative transfers were obtained using all tested enterobacterial species as rec ...201122092811
susceptibility of esbl-producing enterobacteriaceae with the new clsi breakpoints.in 2010 clsi lowered the susceptibility breakpoints of some cephalosporins and aztreonam for enterobacteriaceae and eliminated the need to perform esbl screening and confirmatory tests. the aim of this study was to determine how many esbl-producing strains will test susceptible for three common species of enterobacteriaceae using the new breakpoints. as determined with the clsi screening and confirmatory tests, 382 consecutive esbl-producing strains were collected at our hospital between 2007 an ...201121752977
Bactericidal properties of pradofloxacin against veterinary pathogens.Pradofloxacin is a new veterinary 8-cyano-fluoroquinolone developed for use against bacterial infections in dogs and cats involving both aerobic and anaerobic bacteria. The minimal bactericidal concentrations have been determined against clinical isolates of Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus canis, Proteus spp., Fusobacterium spp., Porphyromonas gingivalis and Prevotella species. A subset of these species was selected, ...201122209121
pcb accumulation and tissue distribution in cave salamander (proteus anguinus anguinus, amphibia, urodela) in the polluted karstic hinterland of the krupa river, slovenia.for over two decades, a manufacturer of electrical capacitors disposed of its waste within the karstic hinterland of the krupa river (slovenia) resulting in the surroundings becomming heavily polluted with pcb. albeit the extent of the contamination has been known since 1983 and the krupa river has become one of the most pcb polluted river in europe, the effects on the cave fauna of the region remain unknown. the most famous cave dweller of the krupa hinterland is the endemic cave salamander pro ...201121658741
lateral sinus thrombosis in children: a review.a retrospective study was undertaken to review the clinical presentation, evaluation, management, and outcome of otogenic lateral sinus thrombosis (lst) in children. all pediatric patients with lst seen in our department between 1999 and 2007 were included; there were 9 cases involving 6 boys and 3 girls whose ages ranged from 8 to 12 years. they had all been treated with antibiotics elsewhere prior to admission, and the duration of symptoms before admission ranged from 5 to 18 days. the most co ...201121674459
Acinetobacter baumannii is Able to Gain and Maintain a Plasmid Harbouring In35 Found in Enterobacteriaceae Isolates From Argentina.The aim of this study was to determine the presence of bla (CTX-M-2) in our A. baumannii population and their putative role as an alternative mechanism of resistance to third-generation cephalosporins in this species. The bla (CTX-M-2) gene is widespread among the Enterobacteriaceae isolates from our country; however, it was not found in 76 isolates A. baumannii non-epidemiologically related clinical isolates resistant to third-generation cephalosporins ...201122119898
Effective cohorting and "superisolation" in a single intensive care unit in response to an outbreak of diverse multi-drug-resistant organisms.Cohorting patients in dedicated hospital wards or wings during infection outbreaks reduces transmission of organisms, yet frequently, this may not be feasible because of inadequate capacity, especially in the intensive care unit (ICU). We hypothesized that cohorting isolation patients in one geographic location in a single ICU and using enhanced isolation procedures ("superisolation") can prevent the further spread of highly multi-drug-resistant organisms (MDRO).201121936667
control of clinical pathogens by the haemolymph of paratelphusa hydrodromous, a freshwater crab.in the present study, effort has been made to find the antimicrobial activity of haemolymph collected from freshwater crab, paratelphusa hydrodromous. the haemolymph collected was tested for antimicrobial assay by disc diffusion method against clinical pathogens. five bacterial species, namely, escherichia coli, klebsiella pneumonia, proteus mirabilis, pseudomonas aeruginosa, staphylococcus aureus, and five fungal strains, namely and aspergillus flavus, aspergillus fumigatus, aspergillus niger, ...201122084719
Antibacterial activity of some Indian Ayurvedic preparations against enteric bacterial pathogens.In Ayurveda, various herbal preparations are clinically used to prevent or cure infectious diseases. Herbal preparations such as Triphala churna, Hareetaki churna, Dashmula churna, Manjistadi churna, Sukhsarak churna, Ajmodadi churna, Shivkshar pachan churna, Mahasudarshan churna, Swadist Virechan churna and Pipramool churna were investigated by preparing their organic solvent extract for antibacterial potential against enteric bacterial pathogens such as Escherichia coli, Staphylococcus aureus, ...201122171288
prevalence of sxt/r391-like integrative and conjugative elements carrying blacmy-2 in proteus mirabilis.objectives to characterize the vectors involved in the dissemination of bla(cmy-2) genes in clinical isolates of proteus mirabilis collected between 1999 and 2007. methods plasmid analysis of 19 p. mirabilis carrying ampc genes was performed by pcr-based replicon typing, s1-pfge and southern hybridization with ampc and replicon probes. isolates that could not be characterized were examined for the presence of sxt/r391-like elements. to demonstrate the involvement of these elements in the dissemi ...201121752830
antimicrobial activity of two essential oils.the aim of the study was to evaluate the antimicrobial activity of essential oils in vitro for possible application to reduce the content of microorganisms in the air of animal houses. the essential oils of cymbopogon citrarus l. and malaleuca alternifolia l. were screened against bacteria staphylococcus aureus, enterococcus faecium, pseudomonas aeruginosa, escherichia coli, proteus mirabilis and yeast candida albicans. the minimal inhibitory concentration of the active essential oils was tested ...201121739934
Fluorinated s-triazinyl piperazines as antimicrobial agents.A series of 1,3,5-triazine derivatives that contain 4-amino-2-trifluoromethyl-benzonitrile, 8-hydroxyquinoline, and different piperazines as substituents at the carbon atoms of the triazine ring have been synthesized by a simple and efficient synthetic protocol. The chemical structures of the compounds were elucidated with the aid of IR, 1H NMR and 13C NMR spectroscopy, and elemental analysis. The antimicrobial activity of the compounds was tested against seven bacteria (Staphylococcus aureus MT ...201121950158
gram-negative bacillary meningitis in egypt.gram-negative bacillary meningitis (gnbm) is a rare disease with a high rate of mortality.201121527836
antimicrobial resistance patterns of uropathogens among children in istanbul, turkey.urinary tract infections are a common cause of end-stage renal disease in turkey. this prospective study investigated the antibiotic resistance patterns of uropathogens in order to recommend appropriate therapeutic protocols for children with urinary tract infections in istanbul, turkey. between october 2007 and october 2008, children presenting with a first episode of urinary tract infection to a pediatric outpatient clinic were enrolled in the study. urine samples were cultured, and antimicrob ...201121710858
[russian results of the international epidemiological study of clinical aspects and resistance to antimicrobial medicines of cystitis uropathogens in females (aresc): significance of empiric therapy].a total of 656 female patients from russia aged between 18 to 65 years with uncomplicated cystitis entered the international aresc trial, of them 647 were eligible for final analysis. positive cultural urine tests ( > 10(4) cfu/ml) were in 419 (64.7%) patients, 393 (93.8%) patients had monoinfection. the central laboratory of genoa (to which the isolated samples were sent) has tested 416 uropathogens from 399 patients. the following pathogens were isolated: e. coli (72.6%), enterococcus (7.0%), ...201121815455
inhibition of inos reduces the therapeutic effects of ozone in acute necrotizing pancreatitis: an in vivo animal study.abstract objective. previously, it was shown that ozone and s-methylthiourea (smt) treatments had ameliorative effects on experimental models of acute necrotizing pancreatitis (anp). it is possible that the combination of ozone and smt may be more effective than either therapy. therefore, we investigated the efficacy of combination therapy with ozone and smt in an experimental rat model of anp. material and methods. sprague-dawley rats were divided into five experimental groups. groups were desi ...201121463160
jacaranda cuspidifolia mart. (bignoniaceae) as antibacterial agent.abstract this study evaluated, in vitro, the antimicrobial activity of the hexane extract (jche), methanol extract (jcme), and chloroform fraction (jccf) of bark from jacaranda cuspidifolia mart. (family bignoniaceae), a brazilian medicinal plant, traditionally used as anti-syphilis and anti-gonorrhea treatment. the antimicrobial activity was evaluated using the disc diffusion method followed by the determination of minimum inhibitory concentration (mic) values. jche was not active against the b ...201121663482
chronic suppurative otitis media in children of luanda, angola.aim:  evaluation of clinical characteristics, bacteriology and hearing in paediatric patients with and without chronic suppurative otitis media (csom) in luanda, angola. methods:  interview, clinical examination, ear-discharge culture, open air pure-tone audiometry and brainstem auditory-evoked potentials of 23 outpatients with csom and 23 controls in a paediatric hospital. results:  of the csom vs. control children, 35% vs. 26% had running water, 70% vs. 70% electricity, 64% vs. 0% hiv (p < 0.0 ...201121477130
anti-proliferative activity of meroditerpenoids isolated from the brown alga stypopodium flabelliforme against several cancer cell lines.the sea constitutes one of the most promising sources of novel compounds with potential application in human therapeutics. in particular, algae have proved to be an interesting source of new bioactive compounds. in this work, six meroditerpenoids (epitaondiol, epitaondiol diacetate, epitaondiol monoacetate, stypotriol triacetate, 14-ketostypodiol diacetate and stypodiol) isolated from the brown alga stypopodium flabelliforme were tested for their cell proliferation inhibitory activity in five ce ...201121673894
quantifying selective reporting and the proteus phenomenon for multiple datasets with similar bias.meta-analyses play an important role in synthesizing evidence from diverse studies and datasets that address similar questions. a major obstacle for meta-analyses arises from biases in reporting. in particular, it is speculated that findings which do not achieve formal statistical significance are less likely reported than statistically significant findings. moreover, the patterns of bias can be complex and may also depend on the timing of the research results and their relationship with previou ...201121479240
[cmy-2-type plasmid-mediated ampc ß-lactamases emerging in tucumán, argentina].in the last years, enterobacteriaceae such as klebsiella pneumoniae, proteus mirabilis and escherichia coli, have acquired resistance to third-generation cephalosporins (c3g) because of the presence of plasmid-mediated ampc ß-lactamases. the aim of this work was to detect plasmid ampc enzymes and to investigate the predominant types in our region. between march and july 2009, 733 consecutive isolates of enterobacteriaceae derived from hospitals and outpatient centers were studied. susceptibility ...201121491062
[urinary tract infection in patient after brain injury].intruduction: one of the most important problems in treating patients after head injury is urinary complication. the first place take urinary infections, which disturb rehabilitation process. clean intermittent catherization, bladder training and good care do not preserve infection. the purpose of our study was to present the frequency of urinary infections and main pathogens involved in these infections among patients treated in the department of rehabilitation after head injury.201121812229
[biochemical criteria for severity of colonic dysbacteriosis].the purpose of the investigation was to estimate the content of malondialdehyde (mda) in the coprofiltrates of human beings with bacteriologically verified colonic dysbacteriosis and to clarify a relationship of the amount of mda in the coprofiltrates to the degree of the abnormal qualitative and quantitative composition of microbiocenosis. specimens from 109 subjects aged 19 to 56 years were examined. the change in the level of mda in the coprofiltrates was found to correspond to the impairment ...201121506382
inhibition of development, swarming differentiation and virulence factors in proteus mirabilis by an extract of lithrea molleoides and its active principle (z,z)-5-(trideca-4',7'-dienyl)-resorcinol.antibacterial activity of lithrea molleoides extract against proteus mirabilis has been previously reported by our group. in the present study, the compound (z,z)-5-(trideca-4',7'-dienyl)-resorcinol (1) was isolated as its responsible active principle. the effects of the compound obtained and of l. molleoides extract on p. mirabilis growth and virulence factors were evaluated. compound 1 showed mic and mbc values of 4000μg/ml. it was found that the extract, at four times the mic, produced comple ...201121514124
microbial isolation and emergence of antimicrobial resistance associated with tigecycline usage.background: with the increasing experience of tigecycline usage, its ecological impact on microorganisms raises concerns but remains unknown. we aimed to analyze the difference in microorganisms isolated before, during, and after tigecycline usage and their susceptibility to antimicrobial agents. methods: between july 2008 and august 2009, 66 patients who received tigecycline monotherapy for more than 2 days at a taiwan medical center were enrolled. antimicrobial susceptibility testing was perfo ...201121524975
the free-radical damage theory: accumulating evidence against a simple link of oxidative stress to ageing and lifespan.recent work on a small european cave salamander (proteus anguinus) has revealed that it has exceptional longevity, yet it appears to have unexceptional defences against oxidative damage. this paper comes at the end of a string of other studies that are calling into question the free-radical damage theory of ageing. this theory rose to prominence in the 1990s as the dominant theory for why we age and die. despite substantial correlative evidence to support it, studies in the last five years have ...201121290398
in vitro antimicrobial and antioxidant activity of acetone and methanol extracts from thymus leucotrichius (lamiaceae).thymus species has been used as tonic and herbal tea, antiseptic, antitussive, carminative, antioxidant, anti-inflammatory and antimicrobial activities. the acetone and methanol extracts of thymus (t.) leucotrichius (labiatae/lamiaceae) was examined for antimicrobial and antioxidant properties.201121796869
diversity of somatic coliphages in coastal regions with different levels of anthropogenic activity in sao paulo state, brazil.bacteriophages are the most abundant and genetically diverse viruses on earth, with their complex ecology in both quantitative and qualitative terms. somatic coliphages (sc) have been reported to be good indicators of fecal pollution in seawater. this study focused on determining the concentration of sc and their diversity by electron microscopy in seawater, plankton and bivalve samples collected at three coastal regions são paulo, brazil. the sc counts varied from <1 to 3.4×10(3) pfu/100ml in s ...201121531842
synthesis of novel fluconazoliums and their evaluation for antibacterial and antifungal activities.a series of novel fluconazoliums were synthesized and their bioactive evaluation as potential antibacterial and antifungal agents were described. some target compounds displayed good and broad-spectrum antimicrobial activities with low mic values ranging from 0.25 to 64 µg/ml against all the tested strains, including three gram-positive bacteria (staphylococcus aureus, mrsa and bacillus subtilis), three gram-negative bacteria (escherichia coli, pseudomonas aeruginosa and bacillus proteus) as wel ...201121794961
synthesis and studies of novel 2-(4-cyano-3-trifluoromethylphenyl amino)-4-(quinoline-4-yloxy)-6-(piperazinyl/piperidinyl)-s-triazines as potential antimicrobial, antimycobacterial and anticancer agents.a series of novel s-triazine analogs were synthesized and characterized by ir, (1)h nmr, (13)c nmr, (19)f nmr spectroscopy and elemental analysis. preliminary screening of target compounds against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberculosis ...201121794959
identifying patients harboring esbl-producing enterobacteriaceae on hospital admission: derivation and validation of a scoring system.increases in community-acquired infections caused by extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae have important implications for hospital infection control and empirical antibiotic therapy protocols. we developed and validated a tool for identifying patients harboring these organisms at hospital admission. we retrospectively analyzed chart data for 849 adult inpatients. the derivation cohort included 339 patients admitted to a large rome hospital during 2008, with (n=113) o ...201121537020
identity gene expression in proteus mirabilis.swarming colonies of independent proteus mirabilis isolates recognize each other as foreign and do not merge together whereas apposing swarms of clonal isolates merge with each other. swarms of mutants with deletions in the ids gene cluster do not merge with their parent. thus ids genes are involved in the ability of p. mirabilis to distinguish self from non-self. here we have characterized expression of the ids genes. we show that idsabcde and f are transcribed as an operon, and we define the p ...201121551301
clinical features and long-term follow-up of quasi-moyamoya disease in children.background: inherited or acquired disorders and conditions may present in conjunction with moyamoya disease. this condition is known as quasi-moyamoya disease. methods: a retrospective review of 69 moyamoya disease patients treated for the past 20 years identified 7 patients with quasi-moyamoya disease and 5 of them were pediatric patients. results: the mean age at initial diagnosis was 6.4 years (range 5-9). associated disorders were: craniosynostosis, dwarfism with coarctation of aorta, proteu ...201121576916
chemical composition and antimicrobial activity of the essential oil of rosemary.the composition of the essential oil of rosemary was analyzed by gas chromatography-mass spectrometry (gc-ms). 22 components, which constitute 97.41% of the oil, were identified. the major constituents were 1,8-cineole (26.54%) and a-pinene (20.14%). minimum inhibitory concentrations (mics), minimal bactericidal concentration (mbc) and time-kill dynamic processes against three gram-positive bacteria (staphylococcus epidermidis, staphylococcus aureus and bacillus subtilis), three gram-negative ba ...201121787731
new inhibitors of colony spreading in bacillus subtilis and bacillus anthracis.we have recently characterized sliding motility in bacillus subtilis strains that lack functional flagella, and here describe the discovery of inhibitors of colony spreading in these strains as well as the aflagellate pathogen, bacillus anthracis. aflagellate b. subtilis strains were used to screen for new types of antibacterials that might inhibit colony spreading on semi-solid media. from a diverse set of organic structures, p-nitrophenylglycerol (npg), an agent used primarily in clinical labo ...201121784632
surgical approach to extensive hidradenitis suppurativa.background hidradenitis suppurativa (hs) is a chronic suppurative disease of skin with high recurrence. objective to determine factors affecting complications and recurrence of hs in patients who underwent surgery. materials and methods we operated on 15 patients with hs at 36 sites between 1999 and 2009. the affected areas were classified as groin, axilla, buttocks, nuchae, perianal, and perineal. all patients were treated using wide surgical excision under general anesthesia. the methods of re ...201121605246
new delhi metallo-ss-lactamase-1: local acquisition in ontario, canada, and challenges in detection.new delhi metallo-ß-lactamase-1 (ndm-1) is a recently identified metallo-ß-lactamase that confers resistance to carbapenems and all other ß-lactam antibiotics, with the exception of aztreonam. ndm-1 is also associated with resistance to many other classes of antibiotics. the enzyme was first identified in organisms isolated from a patient in sweden who had previously received medical treatment in india, but it is now recognized as endemic throughout india and pakistan and has spread worldwide. t ...201121624908
antibacterial activity of selected ethnomedicinal plants from south india.to screen the antimicrobial potential of three ethnomedicinal plants chassalia curviflora thw. (c. curviflora), cyclea peltata hook. f. & thomson (c. peltata) and euphorbia hirta l (e. hirta) used in folk medicines in aarukani hills kani tribe, tamil nadu, india against human bacterial pathogens.201121771680
antibacterial activity of leaves and inter-nodal callus extracts of mentha arvensis l.to determine the anti-bacterial efficacy of chloroform, ethanol, ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from mentha arvensis (m. arvensis) against salmonella typhi(s. typhi), streptococcus pyogenes(s. pyogenes), proteus vulgaris(p. vulgaris) and bacillus subtilis(b. subtilis).201121771452
synthesis of p-triazinylphosphonium salts--hybrid molecules with potential antimicrobial activity.the new hybrid drugs combining in a single molecule triazine ring attached to phosphonium salt were prepared and their bactericidal activity against gram-positive bacteria staphylococcus aureus atcc43300 (mrsa - methycyline resistant staphylococcus aureus strain) two gram-negative bacteria escherichia coli ccug31997 serotype o153 (epec - enteropathogenic escherichia coli strain), proteus mirabilis 1784 (mdr - multidrug resistant clinical strain) were determined using microdilution method.201121648193
evolution and spread of a multidrug-resistant proteus mirabilis clone with chromosomal ampc-type cephalosporinases in europe.proteus mirabilis isolates obtained in 1999 to 2008 from three european countries were analyzed; all carried chromosomal ampc-type cephalosporinase bla(cmy) genes from a citrobacter freundii origin (bla(cmy-2)-like genes). isolates from poland harbored several bla(cmy) genes (bla(cmy-4), bla(cmy-12), bla(cmy-14), bla(cmy-15), and bla(cmy-38) and the new gene bla(cmy-45)), while isolates from italy and greece harbored bla(cmy-16) only. earlier isolates with bla(cmy-4) or bla(cmy-12), recovered in ...201121402851
a computer-assisted diagnostic and treatment concept to increase accuracy and safety in the extracranial correction of cranial vault asymmetries.purpose: proteus syndrome is described as a progressive, asymmetric, disproportional overgrowth of various parts of the body. the theory of somatic mosaicism is widely accepted to be the cause of this disease. affected patients present very heterogeneous symptoms, but in about 30% craniofacial deformities are the leading clinical features. because no causal therapy exists, treatment options are limited to surgical improvement of functional constraints. materials and methods: a computer-assisted ...201121807449
potential use of 68ga-apo-transferrin as a pet imaging agent for detecting staphylococcus aureus infection.(67)ga citrate has been extensively used to detect infection and inflammation since 1971. however, its clinical utility is compromised due to several limitations. the present project explored whether (68)ga-apo-transferrin ((68)ga-tf), when prepared in vitro, is a useful agent for positron emission tomography (pet) imaging of bacterial infection.201121492788
microbiology at first visit of moderate-to-severe diabetic foot infection with antimicrobial activity and a survey of quinolone monotherapy.samples from 1295 patients with diabetic foot infection were evaluated; 4332 samples were collected with an average of 3.3 samples per patient. fifty-seven percent of patients had a 2b ulcer and 23% had a 3b ulcer according to texas university classification. in 64.2% of samples collected at first visit an etiologic agent was identified. about 40% of the positive samples were polymicrobial. gram positive bacteria were more frequently isolated (52.6%), staphylococcus aureus was the most frequentl ...201121824673
microbiological profile with antibiotic sensitivity pattern of cholesteatomatous chronic suppurative otitis media among children.chronic suppurative otitis media (csom) is the most common cause of childhood hearing impairment in the developing countries and atticoantral type is associated with increased incidence of intracranial and extracranial complications. this study was undertaken to define the microbiology of atticoantral type of chronic otitis media and the antibiotic sensitivity pattern, thereby reducing the potential risks of complications.201121715027
composition and antimicrobial activity of achillea distans essential oil.the essential oil of achillea distans w. et k. flower heads was analyzed by gc and gc-ms. altogether 43 components in concentrations more than 0.1% were identified representing 93.5% of the oil composition. the main constituents were 1,8-cineole (16.8%), trans-thujone (9.8%), sabinene (8.2%), borneol (7.5%), beta-pinene (6.5%), and camphor (5.8%). the oil showed moderate activity against staphylococcus aureus and candida albicans, and weak activity against salmonella typhimurium, proteus vulgari ...201121815436
proteus syndrome: a medical rarity. 201121804811
epidemiology of ciprofloxacin resistance and its relationship to extended-spectrum +¦-lactamase production in proteus mirabilis bacteremia.we evaluated the clinical features of ciprofloxacin-resistant proteus mirabilis bacteremia and risk factors for ciprofloxacin resistance.201121437168
molecular analysis of ndm-1-producing enterobacterial isolates from geneva, switzerland.objectives to analyse the mechanisms responsible for decreased susceptibility or resistance to carbapenems in several enterobacterial isolates recovered in 2009-10 in geneva university hospitals, switzerland. methods pcr and sequencing were used to identify β-lactamases, 16s rna methylases and plasmid-mediated quinolone resistance genes. the transferable properties of the plasmids were analysed, as well as their plasmid type. the strains were typed by multilocus sequence typing. results three pa ...201121628303
re: strategy to control catheter encrustation with citrated drinks: a randomized crossover study. a. khan, f. housami, r. melotti, a. timoney and d. stickler. j urol 2010; 183: 1390-1394. 201121439588
setting interpretive breakpoints for antimicrobial susceptibility testing using disk diffusion.antimicrobial susceptibility testing plays a key role in clinical microbiology. the disk diffusion test dates back to the 1940s and became standardised from the 1950s, with the international collaborative study (ics) and national committee for clinical laboratory standards (nccls) as the two major standards. interlaboratory variation of disk test results was recognised early but has never been dealt with in a satisfactory manner. the error-rate bounded method was described in 1974 and its role i ...201121640560
recognition of dna by the helix-turn-helix global regulatory protein lrp is modulated by the amino terminus.the asnc/lrp family of regulatory proteins links bacterial and archaeal transcription patterns to metabolism. in escherichia coli lrp regulates approximately 400 genes, over 200 of them directly. in earlier studies, lrp genes from vibrio cholerae, proteus mirabilis, and e. coli were introduced into the same e. coli background, and yielded overlapping but significantly different regulons. these differences were seen despite amino acid sequence identities of 92% (vibrio) and 98% (proteus) to e. co ...201121642464
production of bioactive sheep β-defensin-1 in pichia pastoris.previous research has shown that sheep β-defensin-1 (sbd-1), a small cationic peptide with a broad range of antimicrobial activities, could inhibit the growth of both gram-positive and gram-negative bacteria as well as that of fungi. in order to increase the yield of current ovine defensin purification methods, mature sbd-1 (msbd-1) was added with a 6-his tag on the c-terminus (msbd-1-t) and expressed in pichia pastoris in the presented work. the msbd-1 and msbd-1-t were expressed in the pichia ...201121643707
[practice management of chronic bacterial prostatitis with levofloxacin].the aim of this prospective observational study was to present relevant data on the efficacy and safety of levofloxacin in the treatment of chronic bacterial prostatitis (nih-ii) in the daily clinical routine of urologists in their practices. in total, 243 patients were treated, including 62 patients with a microbiologically confirmed diagnosis -based on expressed prostatic secretion (eps). the current evaluation is based on these patients. their median age was 53 years. the patients were treate ...201121445824
in vitro synthesis of biogenic amines by brochothrix thermosphacta isolates from meat and meat products and the influence of other microorganisms.twenty brochothrix thermosphacta strains tested for biogenic amines (bas) production, formed histamine (6.6-16.2 mg/kg) and tyramine (18.7-35.4 mg/kg) but neither putrescine nor cadaverine. six of the twenty strains were also investigated in respect of their influence on the synthesis of bas by escherichia coli, pseudomonas sp., proteus mirabilis and lactobacillus sakei. in pure culture escherichia coli produced all of the studied amines (histamine, tyramine, putrescine and cadaverine) with a to ...201121382674
antimicrobial resistance in urinary tract pathogens in canada from 2007 to 2009: canward surveillance study.from january 2007 to december 2009 an annual canadian national surveillance study (canward) tested 2943 urinary culture pathogens for antimicrobial susceptibilities following clinical and laboratory standards institute (clsi) guidelines. the most frequently isolated urinary pathogens (n, % of all isolates) were escherichia coli (1581, 54%), enterococci (410, 14%), klebsiella pneumoniae (274, 9%), proteus mirabilis (122, 4%), pseudomonas aeruginosa (100, 3%), and staphylococcus aureus (80, 3%). r ...201121537027
in vitro antimicrobial efficacy of a silver alginate dressing on burn wound isolates.to test the antimicrobial effectiveness of a silver alginate dressing on opportunistic pathogens, namely meticillin-sensitive staphylococcus aureus (mssa) and meticillin-resistant staphylococcus aureus (mrsa), klebsiella spp., enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, escherichia coli, enterobacter sakazakii, enterobacter cloacae, serratia marcescens, chryseobacterium indologenes, proteus vulgaris and acinetobacter baumannii.201121537295
synthesis and antimicrobial activities of hexahydroimidazo[1,5-a]pyridinium bromides with varying benzyl substituents.variously substituted benzyl bromides were employed to quaternize hexahydrobenzylimidazo[1,5-a]pyridine (a) and the resulting bromides (1-11) were evaluated for their in vitro antimicrobial activity against 10 pathogenic microorganisms: staphylococcus aureus, staphylococcus epidermidis, bacillus cereus, micrococcus luteus, proteus vulgaris, escherichia coli, salmonella typhimurium, klebsiella pneumonia, candida albicans and candida krusei. antimicrobial activities were surprisingly high (mic: 0. ...201121543140
cold atmospheric plasma: a successful treatment of lesions in hailey-hailey disease. 201121482888
bacteriologic and plasmid analysis of etiologic agents of conjunctivitis in lagos, nigeria.background: conjunctivitis, an inflammation of the conjunctiva, is one of the most common eye problems affecting all age groups in nigeria. a better understanding of its epidemiology and the antibiotic susceptibility of etiologic bacterial agents is crucial for the initiation of preventive and therapeutic measures. this study determined the distribution and patterns of bacterial infections in nigerian patients with conjunctivitis. antibiotic resistance patterns and the plasmid profiles of these ...201121484175
phytochemical screening and antibacterial activities of vernonia ambigua, vernonia blumeoides and vernonia oocephala (asteraceae).some vernonia species (vernonia ambigua, vernonia blumeoides and vernonia oocephala) used in northern nigerian traditional medicine, were subjected to phytochemical screening using standard procedures. the antibacterial activity using the disc diffusion method as outlined by the nccls was carried out on klebsiella pneumoniae, streptococcus pyogenes, staphylococcus aureus, corynbacterium ulcerans, methicillin resistant staphylococcus aureus (mrsa), salmonella typhi, pseudomonas aeruginosa, shigel ...201121485703
ß-lactamase producing enterobacteria isolated from surveillance swabs of patients in a cardiac intensive care unit in rio de janeiro, brazil.there is a high incidence of infections caused by betalactamase-producing gram-negative microorganisms in brazil. these organisms are of clinical and epidemiological importance, since their mobile genetic elements facilitate cross-infection. the present study was conducted in sentinel rectal swabs from patients admitted to a cardiac surgery hospital in rio de janeiro, from january through december 2007, in a consecutive manner. the aim of the study was to characterize the genotype and phenotype ...201121412586
[beta-lactamases of clinical isolates of enterobacteriaceae family].among 62 clinical strains of enterobacteria, isolated from intestinal tract, representatives of genus escherichia made--67.4%, klebsiella--4.84%, citrobacter--4.84%, enterobacter--8.06%, proteus--6.45%, morganella of 8.06%. preparations of fluorquinolone group--gatifloxacin and moxifloxacin, the number of steady strains being from 0.0% to 33.3%, were the most effective in respect of enterobacteria the investigated strains of enterobacteria showed considerable resistance to beta-lactam antibiotic ...201121598655
in vitro antimicrobial activity of ten medicinal plants against clinical isolates of oral cancer cases.abstract: background: suppression of immune system in treated cancer patients may lead to secondary infections that obviate the need of antibiotics. in the present study, an attempt was made to understand the occurrence of secondary infections in immuno-suppressed patients along with herbal control of these infections with the following objectives to: (a) isolate the microbial species from the treated oral cancer patients along with the estimation of absolute neutrophile counts of patients. (b) ...201121599889
screening of australian plants for antimicrobial activity against campylobacter jejuni.campylobacter jejuni is the most common cause of acute enteritis in humans, with symptoms such as diarrhoea, fever and abdominal cramps. in this study, 115 extracts from 109 australian plant species were investigated for their antimicrobial activities against two c. jejuni strains using an in vitro broth microdilution assay. among the plants tested, 107 (93%) extracts showed activity at a concentration between 32 and 1024 µg/ml against at least one c. jejuni strain. seventeen plant extracts were ...201121604309
pulmonary manifestations in proteus syndrome: pulmonary varicosities and bullous lung disease.we report on two patients with proteus syndrome (ps), with emphasis on its pulmonary manifestations. the first patient was a 6-year-old girl diagnosed with ps at 5 years of age. the pulmonary abnormalities first observed at age 3 years and included streaky densities with accentuated vascular markings detected by chest radiography. the patient had persistent abnormalities on follow-up chest radiographs. chest computed tomography (ct) scans showed diffuse pulmonary venous dilatations. the second p ...201121412980
a novel compound from the marine bacterium bacillus pumilus s6-15 inhibits biofilm formation in gram-positive and gram-negative species.biofilm formation is a critical problem in nosocomial infections and in the aquaculture industries and biofilms show high resistance to antibiotics. the aim of the present study was to reveal a novel anti-biofilm compound from marine bacteria against antibiotic resistant gram-positive and gram-negative biofilms. the bacterial extract (50 μg ml(-1)) of s6-01 (bacillus indicus = mtcc 5559) showed 80-90% biofilm inhibition against escherichia coli, shigella flexneri, proteus mirabilis and s6-15 (ba ...201121614700
antimicrobial activity of protease inhibitor from leaves of coccinia grandis (l.) voigt.antimicrobial activity of protease inhibitor isolated from coccinia grandis (l.) voigt. has been reported. a 14.3 kda protease inhibitor (pi) was isolated and purified to homogeneity by ammonium sulfate precipitation (20-85% saturation), sephadex g-75, deae sepharose column and trypsin-sepharose affinity chromatography from the leaves of c. grandis. the purity was checked by reverse phase high performance liquid chromatography. pi exhibited marked growth inhibitory effects on colon cell lines in ...201121615062
neurofibromatosis type 1: persisting misidentification of the "elephant man" disease.during informal interviews in the course of an ethnographic study on intergenerational dialogue between individuals with neurofibromatosis and their parents, many members of canadian neurofibromatosis associations have stated that they continue to be told the condition that afflicts them or their children is "elephant man's disease." today, even though well-established clinical criteria make it possible to diagnose and differentiate the 2 diseases, the confusion between neurofibromatosis type 1 ...201121209351
antimicrobial activity of organotin(iv) complexes with the ligand benzil bis(benzoylhydrazone) and 4,4'-bipyridyl as coligand.several methyltin(iv) and butyltin(iv) complexes with the ligand benzil bis(benzoylhydrazone) and 4,4'-bipyridyl as coligand were synthesised and characterized by elemental analysis and by ir, (1)h, (13)c and (119)sn nmr spectroscopies. some of them were also analyzed using single crystal x-ray diffraction. the title compounds were evaluated for their in vitro antimicrobial properties. all buthyltin complexes showed significant inhibition of gram positive bacteria, resulting bacillus subtilis, s ...201121443849
urine cultures from indwelling bladder catheters in nursing home patients: a point prevalence study in a swedish county.abstract objectives. to survey the bacterial flora and antibiotic resistance in urinary strains from patients with indwelling bladder catheters residing in nursing homes within a geographically defined region. material and methods. urine was sampled for culture from 163 catheter patients (126 men and 37 women) during a 2 week period in march 2010. susceptibility testing of the isolated bacteria was compared with all urinary strains (n = 9994) from hospitals and primary healthcare in the same geo ...201121452928
urease inhibitors as potential drugs for gastric and urinary tract infections: a patent review.introduction: urease is the enzyme that catalyzes the hydrolysis of urea, which is involved in serious infections caused by helicobacter pylori in the gastric tract, as well as proteus and related species in the urinary tract. the necessity to treat such infections has stimulated intensive studies on various groups of urease inhibitors. areas covered: patent literature on urease inhibitors with possible applications in medicine is reviewed in this paper. hydroxamic acids, phosphoramidates, urea ...201121457123
[chronic pyelonephritis and xanthogranulomatous pyelonephritis]. 201121458176
identification of bacteria isolated from nasal polyps and their ability to produce superantigens and biofilms in lebanese patients.staphylococcus aureus superantigens and bacterial biofilms have been implicated in the development of chronic rhinosinusitis and nasal polyps. we conducted a study of 32 lebanese patients-21 males and 11 females, aged 15 to 71 years (mean: 39)-to identify bacteria isolated from nasal polyps and to determine if these bacteria produced superantigens and biofilms. polyps were surgically removed, homogenized, and subjected to bacteriologic studies. the presence or absence of s aureus enterotoxin a, ...201121500161
synergistic combination of chitosan acetate with nanoparticle silver as a topical antimicrobial: efficacy against bacterial burn infections.chitosan and nanoparticle silver are both materials with demonstrated antimicrobial properties and have been proposed singly or combined as constituents of antimicrobial burn dressings. here we show that they combine synergistically together to inhibit the in vitro growth of gram-positive methicillin-resistant staphylococcus aureus (mrsa) and gram-negative bacteria (pseudomonas aeruginosa, proteus mirabilis and acinetobacter baumannii) as judged by bioluminescence monitoring and isobolographic a ...201121502618
antibiotic susceptibility and imaging findings of the causative microorganisms responsible for acute urinary tract infection in children: a five-year single center study.we studied the differences in the antibiotic susceptibilities of the microorganisms that causeing urinary tract infections (uti) in children to obtain useful information on appropriate drug selection for childhood uti.201121503201
sitafloxacin: in bacterial infections.sitafloxacin is a fluoroquinolone antibacterial with in vitro activity against a broad range of gram-positive and -negative bacteria, including anaerobic bacteria, as well as against atypical pathogens. it is approved in japan for use in a number of bacterial infections caused by sitafloxacin-susceptible strains of staphylococcus spp., streptococcus pneumoniae, other streptococcus spp., enterococcus spp., moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., enterobacter sp ...201121504249
transcriptome of proteus mirabilis in the murine urinary tract: virulence and nitrogen assimilation gene expression.the enteric bacterium proteus mirabilis is a common cause of complicated urinary tract infections. in this study, microarrays were used to analyze p. mirabilis gene expression in vivo from experimentally infected mice. urine was collected at 1, 3, and 7 days postinfection, and rna was isolated from bacteria in the urine for transcriptional analysis. across 9 microarrays, 471 genes were upregulated and 82 were downregulated in vivo compared to in vitro broth culture. genes upregulated in vivo enc ...201121505083
carijodienone from the octocoral carijoa multiflora. a spiropregnane-based steroid.two new steroids (1 and 2) and the known pregna-1,4,20-trien-3-one (3) have been isolated from the pacific octocoral carijoa multiflora. compound 1 possesses a novel spiropregnane-based steroidal skeleton. the photochemical transformation of 3 into 1 allowed the assignment of the absolute configuration at c-10 of 1. the antibacterial activities of compounds 1 and 3 were evaluated against a panel of bacterial strains.201121235217
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