TitleAbstractYear(sorted ascending)
plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing enterobacteriaceae in morocco.although independently acquired, plasmid-mediated quinolone resistance appears to be linked with extended-spectrum or ampc-type beta-lactamases. since no data are available in african countries, the prevalence of qnr genes at the university hospital ibn rochd, casablanca, morocco, was investigated.201021252459
assessment of effectiveness and safety of ibicella lutea extract in the control of experimental proteus mirabilis urinary tract infection.proteus mirabilis is an important cause of complicated urinary tract infections (uti). like many other microorganisms, p. mirabilis has acquired resistance to many antibiotics. due to the serious effects associated with uropathogenic p. mirabilis and the problems related to the use of antibiotics, alternative strategies for its control must be developed. previously, we studied the effect of ibicella lutea extract, a south american indigenous plant, on in vitro uropathogenicity of p. mirabilis. w ...201021252462
microbial evaluation and public health implications of urine as alternative therapy in clinical pediatric cases: health implication of urine therapy.cultural means of pediatric treatment during ill health is a mainstay in africa, and though urine has been known to contain enteric pathogens, urine therapy is still culturally applicable in some health conditions and also advocated as alternative therapy. the study therefore, is to evaluate the microbial contents and safety of urine.201021293739
bacteremia in a multilevel geriatric hospital.bloodstream infection (bsi) is a major cause of morbidity and mortality in hospitals. bacteremia in the elderly remains a diagnostic as well as a therapeutic challenge to the clinician.201021333922
akara ogbomoso: microbiological examination and identification of hazards and critical control points.akara ogbomoso was examined toward the establishment of hazard analysis and critical control point (haccp). the akara was produced in residential buildings with the attendant consequence of contamination. there was ample growth of aerobes, coliforms, staphylococci, shigella and yeast/mold from the samples, water and cowpea pastes. microbial contaminations occur through the processing, which can be corrected through education by adopting good hygienic and manufacturing practices. the critical con ...201021339157
prevalence of pseudomonas aeruginosa in post-operative wound infection in a referral hospital in haryana, india.the objective of our study was to determine the prevalence of pseudomonas aeruginosa in the isolates of postoperative wound and its susceptibility pattern to commonly used antibiotics.201021346900
[immunovac-vp-4 for prevention of acute respiratory diseases in children's communities].assessment of prophylactic effect of immunovac-vp-4 against acute respiratory diseases (ards) in collectives of children.201021381375
a case of disproportionate macrodactyly or a mild form of proteus syndrome? an interesting case.we present a 20-year-old malay male whom we believe has proteus syndrome, a rare congenital disorder of asymmetrical overgrowth of body tissues. there are fewer than 100 confirmed cases reported worldwide thus the clinical presentation and histopathological findings are of significance. our patient presented with an overgrown right small finger and subcutaneous purplish pigmentation over his left upper arm and chest since birth. his small finger gradually increased in size. he had no abnormaliti ...201021400985
antibiotic susceptibility pattern of urinary tract pathogens in ibadan, nigeria.urinary tract infections (uti) affect all age groups and occur in both hospitalized and non-hospitalized individuals and have serious impact on the socioeconomic life of the individual and the society, and also account for a large proportion of antibacterial drug consumption. treatment failure has been attributed to increasing resistance to common antibiotics, but there is paucity of data from this part of nigeria. this prospective study was carried out to determine the prevalence and antibiotic ...201021416786
syndromes with focal overgrowth in infancy: diagnostic approach and surgical treatment.abstract syndromes with focal overgrowth are sporadic diseases and comprise proteus syndrome and congenital lipomatous overgrowth, vascular malformations, and epidermal naevi (clove) syndrome, and isolated hemihyperplasia. we describe 3 children classified according to standard criteria with dysregulated growth of various tissues that was excised, together with excess toes, and tumours. correct classification facilitates diagnosis and operations. interdisciplinary treatment and follow-up are rec ...201021446802
lactobacillus fermentum isolated from human colonic mucosal biopsy inhibits the growth and adhesion of enteric and foodborne pathogens.a number of lactobacillus species are used as probiotic strains in order to benefit health. we have isolated l. fermentum from human colonic mucosal biopsy samples that possess antimicrobial activities against entroinvasive and foodborne pathogens such as escherichia coli, salmonella paratyphi a, shigella sonnei, staphylococcus aureus, enterococcus faecalis, proteus mirabilis, pseudomonas aeruginosa, and vibrio sp. in addition to lactic acid, l. fermentum secretes antimicrobial proteinacious com ...201021535608
[antimicrobial resistance pattern for gram-negative uropathogens isolated from hospitalised patients and outpatients in cartagena, 2005-2008].determining the microbial aetiology spectrum and antibiotic resistance pattern of uropathogens causing urinary tract infections in hospitalised patients and outpatients.201022030688
[antimicrobial susceptibility of clinical isolates of aerobic gram-negative bacteria in 2006].we determined mics of antibacterial agents against 1280 clinical strains of aerobic gram-negative bacteria (19 genus or species) isolated at 16 japanese facilities in 2006. mics were determined using mostly broth microdilution method and antibacterial activity was assessed. strains producing extended-spectrum beta-lactamases (esbl) accounted for 3.7% of escherichia coli, 2.7% of klebsiella spp., and 11.4% of proteus spp. notably, 18.8% of proteus mirabilis was found to produce esbl higher than 1 ...201021425597
role of bacteria isolates in the spoilage of fermented african oil bean seed was carried out to determine the spoilage association of traditionally fermented ugba, product of african oil bean seed. samples were collected from three markets within enugu metropolis in eastern nigeria. microbial population of ugba was isolated, characterized and identified. effect of time on product quality, total viable cell count and individual growth pattern of isolates was evaluated. microorganisms isolated were proteus sp., klebsiella sp., staphylococcus epidermidis, e. coli, bac ...201021848074
studies of antimicrobial activities of some 4-thiazolidinone fused pyrimidines, [1,5]-benzodiazepines and their oxygen substituted hydroxylamine derivatives.thiazolidin-4-one fused pyrimidines, [1,5]-benzodiazepines and their oxygen substituted hydroxylamine derivatives have been screened for antibacterial, antifungal and antimalarial activity. bacillus subtilis, escherichia coli, proteus mirabilis and salmonella typhi were used for antibacterial screening. aspergillus fumigatus and candida albicans were used for antifungal screening and plasmodium species were used for antimalarial screening. the antibacterial and antifungal activities are expresse ...201021694993
prolonged bacteraemia caused by vim-1 metallo-beta-lactamase-producing proteus mirabilis: first report from italy.persistent bacteraemia arising from a case of post-operative mediastinitis as a result of a proteus mirabilis isolate, possessing two class 1 integrons carrying bla(vim-1) and aada2 gene cassettes located on chromosomal and plasmidic dna, respectively, is reported. despite the in vitro susceptibility to carbapenems, meropenem therapy failed, whereas the patient responded to treatment with cefepime plus amikacin. to our knowledge, this is the first report of metallo-beta-lactamase production in a ...201019624511
980-nm laser diode and treatment of subcutaneous mass in proteus-like syndrome. 201019627404
detection of ampc beta-lactamases in escherichia coli, klebsiella spp., salmonella spp. and proteus mirabilis in a regional clinical microbiology laboratory.there are currently no standardized diagnostic tests available for the reliable detection of ampc beta-lactamases in klebsiella spp., escherichia coli, proteus mirabilis and salmonella spp. a study was designed to evaluate a confirmation disk test using cefotetan (ctt) and cefoxitin (fox) with phenylboronic acid (pba). it also investigated the most suitable screening concentrations of fox, ceftriaxone (cro) and ceftazidime (caz) for the detection of ampc beta-lactamases. a total of 126 control ( ...201019456838
monozygotic twins discordant for vascular malformations and dysregulated growth.there is a large group of disorders characterized by non-cranial vascular malformations and a dysregulated growth, of which the prototype may be klippel-trenaunay syndrome (kts). the aetiology of kts and vascular malformations-dysregulated growth (vm-dg) syndromes resembling kts is obscure, but polygenic paradominant inheritance involving mutations or polymorphisms in two or more genes simultaneously, of which one (or more) are involved in (lymph)angiogenesis and the other(s) in growth regulatio ...201019716450
gram-negative bacteria associated with brewery yeasts: reclassification of obesumbacterium proteus biogroup 2 as shimwellia pseudoproteus gen. nov., sp. nov., and transfer of escherichia blattae to shimwellia blattae comb. nov.phylogenetic analyses of type and reference strains of obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16s rrna gene sequences and partial sequences of four protein-coding genes (fusa, leus, pyrg and rpob). both approaches revealed that o. proteus biogroup 1 strains were closely related to hafnia alvei. biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included escherichia blattae. phenotypic tests were ...201019661513
syndromes with focal overgrowth in infancy: diagnostic approach and surgical treatment.abstract syndromes with focal overgrowth are sporadic diseases and comprise proteus syndrome and congenital lipomatous overgrowth, vascular malformations, and epidermal naevi (clove) syndrome, and isolated hemihyperplasia. we describe 3 children classified according to standard criteria with dysregulated growth of various tissues that was excised, together with excess toes, and tumours. correct classification facilitates diagnosis and operations. interdisciplinary treatment and follow-up are rec ...201019634071
characterization and flocculation mechanism of high efficiency microbial flocculant tj-f1 from proteus mirabilis.the characterization and the flocculation mechanism of microbial flocculant tj-f1 with high flocculating activity, produced by proteus mirabilis from a mixed activated sludge, was investigated. mainly consisting of protein and acid polysaccharide, tj-f1 owns a molecular weight of 1.2 x 10(5)da, which brings strong van der waals forces and ample binding-sites. it contains carboxyl, hydroxyl, amino groups and hydrogen bonds preferred for the flocculation process. the key parameters influencing the ...201019773148
prevalence of acquired ampc beta-lactamases in enterobacteriaceae lacking inducible chromosomal ampc genes at a spanish hospital from 1999 to 2007, a significant increase in acquired ampc genes in enterobacteriaceae from 0.06% in 1999 to 1.3% was observed. proteus mirabilis showed the highest prevalence (0.95%) and cmy-2 was the most prevalent ampc enzyme (66.7%). other enzymes such as cmy-4, dha-1, acc-1, and three new enzymes called cmy-25, cmy-27 and cmy-40 were detected. seven out of the 117 isolates (6%) also produced an extended-spectrum beta-lactamase. as acquired ampc enzymes are likely to become a serious public health iss ...201019523051
antibacterial activities of crude extract of aloe barbadensis to clinically isolated bacterial pathogens.the antibacterial activity of aloe barbadensis was tested on clinically isolated bacterial pathogens i.e. enterococcus bovis, staphylococcus aureus, escherichia coli, proteus vulgaris, proteus mirabilis, pseudomonas aeruginosa, morganella morganii, and klebsiella pneumoniae causing infection in human being. ethanolic and aqueous extracts were used for the antibacterial effect, which was measured by the appearance of zone of inhibition. relatively higher mic concentrations were obtained for gram ...201019263248
repression of common bull sperm flora and in vitro impairment of sperm motility with pseudomonas aeruginosa introduced by contaminated lubricant.semen collected from clinically healthy bulls at an artificial insemination centre was examined for bacterial diversity. while bacteria that are normally present in the common flora of bovine semen were absent, such as mycoplasma sp., proteus sp. and corynebacterium sp., all semen samples contained an unusually high number of pseudomonas aeruginosa strains. analysis via pulsed field gel electrophoresis demonstrated that one particular p. aeruginosa strain, present in a sealed bottle of lubricant ...201019144031
chronic rectal bleeding in proteus syndrome.proteus syndrome is a rare congenital hamartomatous disorder that typically manifests itself in overgrowth, vascular malformation and disregulation of fatty tissue. the tissues affected are commonly the limbs but can be of any tissue. vascular anomalies are common and appear at random sites on the body. diagnosis is often difficult leading to wrong treatment. we describe a case of a 17-year-old girl with proteus syndrome presented with symptomatic anaemia secondary to chronic rectal bleeding. co ...201021901955
isolation and identification of microbes associated with mobile phones in dammam in eastern saudi arabia.this study was conducted to determine microbial contamination of mobile phones in the city of dammam, in the eastern region of saudi arabia, and identify the most important microbial species associated with these phones in order to take the necessary remedial measures.201022022665
in-vitro activity of three brands of ceftriaxone against different clinical isolates.ceftriaxone is an extended spectrum cephalosporin that has been used for decades for combating infections caused by wide array of pathogens and prescription of antibiotic is in favor of specific brands without realizing the potency of the brand. the in-vitro activity of three brands of ceftriaxone and various antibiotics against clinical isolates is studied.201022049828
genetic features of blandm-1-positive enterobacteriaceae.genetic features associated with the bla(ndm-1) gene were investigated in 6 escherichia coli, 7 klebsiella pneumoniae, 1 citrobacter freundii, 1 proteus mirabilis, and 1 providencia stuartii isolate of worldwide origin. clonal diversity was observed for both e. coli and k. pneumoniae. the bla(ndm-1) gene was carried by different plasmid types (inca/c, incf, incl/m, or untypeable) and was likely chromosome borne in two isolates. the bla(ndm-1) plasmids coharbored a variety of resistance determina ...201121859933
photoletter to the editor: oral ulceration in pyoderma gangrenosum.a 65-year-old woman presented with widespread necrotising cutaneous ulceration and oral involvement. past history included rheumatoid arthritis, and a left nephrectomy.examination revealed multiple violaceous undermined ulcers. blood investigations showed an acute inflammatory response. skin histopathology showed epidermal ulceration with acute and chronic inflammation. direct immunofluorescence was negative. a diagnosis of pyoderma gangrenosum with oral involvement was made. mycophenolate mofet ...201121894254
penile reconstruction for a case of genital lymphoedema secondary to proteus our knowledge penile lymphoedema secondary to proteus syndrome has not previously been reported. hence we report a case of a 16-year-old male who was referred with features of right hemi-hypertrophy and severe lymphoedema affecting his scrotum and penis. he had previously undergone scrotal reduction surgery at the age of 13, but had since developed worsening penile oedema. his main concern was that of cosmetic appearance prior to sexual debut, and he also complained of erectile dysfunction. a ...201122084799
the microbial flora associated with oral carcinomas.changes in the microbial flora on the oral mucosa after cancerous alteration may lead to both local and systemic infections. in this study, we assessed the microbial flora associated with the surfaces of oral squamous cell carcinoma. a comparative evaluation of these microbial contents was made with that of the contralateral healthy mucosa and control (healthy) mucosa. we also assessed the microbial flora from the saliva culture in subjects with oral squamous cell carcinoma and healthy controls.201121909494
the rising problem of antimicrobial resistance in the intensive care unit.abstract: mainly due to its extremely vulnerable population of critically ill patients, and the high use of (invasive) procedures, the intensive care unit (icu) is the epicenter of infections. these infections are associated with an important rise in morbidity, mortality, and healthcare costs. the additional problem of multidrug-resistant pathogens boosts the adverse impact of infections in icus. several factors influence the rapid spread of multidrug-resistant pathogens in the icu, e.g., new mu ...201122112929
Gram stain microbiological pattern of upper extremities suppuration at Baptist Medical Centre, Ogbomoso Nigeria: a fifteen month review.Sixty-eight (68) patients with serious upper extremity suppurative infections, presenting within a period of fifteen (15) months, were prospectively studied clinically, Gram stain of aspirates/pus were performed, specimen cultured, planted, and where indicated glucose levels and haemoglobin genotype determined. Half of the patients had hand infections. Staphylococcus aureus was isolated from thirty-nine (39) patients. Gram Negative bacilli, including Salmonella were more isolated from patients w ...201122195385
Preventing catheter-related urinary tract infection: focus on Proteus mirabilis.Urinary tract infectious caused by Proteus are uncommon, and account for only 1-2% of community-acquired infectious. However, it is important that staff are able to recognise the features of a Proteus infection. Proteus infections, if unrecognised, can become established and cause major health problems. Proteus is more common in people who have or have had a urinary catheter. Minimizing the incidence and duration of urinary catheterization is an important part of preventing infection. Community ...201122145192
Macrodystrophia lipomatosa involving multiple nerves.Macrodystrophia lipomatosa (MDL), a rare congenital disorder, is considered by some to be a localized form of Proteus syndrome. The implication of the PTEN (phosphatase and tensin homolog deleted on chromosome 10) gene in both strengthens this belief. We present a case who had MDL in multiple nerve territories-all on the same side of the body-with hypertrophy of mainly fibroadipose tissue throughout their distribution, thus pointing to a form of localized hemihypertrophy; both hemihypertrophy an ...201121948052
[effect of acetylcholine and acetylcholinesterase on the activity of contractile vacuole of amoeba proteus].acetylcholine (ach, 1 microm) stimulates activity of the contractile vacuole of proteus. the effect of ach is not mimicked by its analogs which are not hydrolyzed by acetylcholinesterase (ache), i. e., carbacholine and 5-methylfurmethide. the effect of ach is not sensitive to the blocking action of m-cholinolytics, atropine and mytolone, but is suppressed by n-cholinolytic, tubocurarine. the inhibitors of ache, eserine (0.01 microm) and armine (0.1 microm), suppress the effect of ach on amoeba c ...201121870511
Structure of the core part of the lipopolysaccharide from Proteus mirabilis genomic strain HI4320.The structure of the core part of the lipopolysaccharide from Proteus mirabilis genomic strain HI4320 was studied. Core oligosaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide and analyzed by NMR spectroscopy and mass spectrometry as well as chemical methods. The structure of the oligosaccharide was established.201121999542
Pulmonary Zygomycosis with Cunninghamella bertholletiae in a Killer Whale (Orcinus orca).An adult female killer whale (Orcinus orca) was transported to the Port of Nagoya public aquarium in June 2010. While the animal was being maintained in the aquarium there was a gradual decrease in body weight. On October 1st, 2010 the whale exhibited signs of gastrointestinal disease and died on January 14th, 2011. At necropsy examination the gastric compartments were filled with a large number of variably-sized rocks (total weight 81.4 kg) and there was marked ulceration in the third compartme ...201122000035
proteus mirabilis biofilms and catheter-associated urinary tract infections.proteus mirabilis inhabits the environment and causes a number of infections including those of the skin, respiratory tract, wounds and urinary tract. these organisms express virulence factors associated with adhesion, motility, immunoavoidance, nutrient acquisition, host damage, as well as biofilm formation. p. mirabilis produces biofilms in diverse habitats with those formed in the human host playing a key role in indwelling device infections. the most studied p. mirabilis biofilms are those f ...201121921687
radial and spiral stream formation in proteus mirabilis colonies.the enteric bacterium proteus mirabilis, which is a pathogen that forms biofilms in vivo, can swarm over hard surfaces and form a variety of spatial patterns in colonies. colony formation involves two distinct cell types: swarmer cells that dominate near the surface and the leading edge, and swimmer cells that prefer a less viscous medium, but the mechanisms underlying pattern formation are not understood. new experimental investigations reported here show that swimmer cells in the center of the ...201122219724
Proteus penneri lurking in the intensive care unit: An important often ignored nosocomial pathogen. 201122013265
Isolation and identification of antibacterial compound from the leaves of Cassia auriculata.Antimicrobial properties of medicinal plants and plant parts such as flowers, roots, fruits, seeds and oils are being used to cure some chronic and acute diseases throughout the world. In the present study, an attempt has been made to isolate and identify the antibacterial compound present in the leaves of the Cassia auriculata.201122013726
[evaluation of biofilm formation by proteus mirabilis strains on the surface of different biomaterials by two methods].proteus sp. rods are opportunistic human pathogens. these microorganisms are mainly isolated from patients with urinary tract infections, particularly associated with using of biomaterials, on which surface they can form biofilm. the aim of our study was the estimation of proteus mirabilis rods ability to form biofilm on the surface of 5 biomaterials (polychloride vinyl, silicone latex, polypropylene, polybutylen teraftalan and polyamide) using richards' and quantitative method and comparison re ...201122184907
anthemis wiedemanniana essential oil prevents lps-induced production of no in raw 264.7 macrophages and exerts antiproliferative and antibacterial activities in vitro.anthemis wiedemanniana is known in folk medicine for the treatment of microbial infections, cancer and also urinary and pulmonary problems. in this study, the chemical composition of the essential oil from a. wiedemanniana was evaluated and its antibacterial activity was tested against 10 bacterial strains. the oil was also tested for its potentiality to inhibit nitric oxide production in raw 264.7 macrophages and for its cytotoxicity against four human cancer cell lines. a. wiedemanniana oil, r ...201122124231
outbreak caused by proteus mirabilis isolates producing weakly expressed tem-derived extended-spectrum β-lactamase in spinal cord injury patients with recurrent bacteriuria.we performed a retrospective extended-spectrum β-lactamase (esbl) molecular characterization of proteus mirabilis isolates recovered from urine of spinal cord injury patients. a incorrectly detected tem-24-producing clone and a new weakly expressed tem-derived esbl were discovered. in such patients, esbl detection in daily practice should be improved by systematic use of a synergy test in strains of p. mirabilis resistant to penicillins.201121888562
Cerebral Abscess Complicating Proteus mirabilis Meningitis in a Newborn Infant.Neonatal bacterial meningitis is associated with high morbidity and mortality, especially when complicated by intracranial abscesses. The authors report the case of an 11-day-old female with Proteus mirabilis meningitis complicated by an asymptomatic brain abscess. With this report, the authors illustrate the importance of surveillance neuroradiologic imaging in patients with Proteus meningitis.201122114212
application of high-throughput sequencing to measure the performance of commonly used selective cultivation methods for the foodborne pathogen campylobacter.campylobacter is an important foodborne human pathogen, which has traditionally been studied using a variety of selective cultivation methods. here we use next-generation sequencing to ask the following: (i) how selective are commonly used campylobacter cultivation methods relative to the initial sample and (ii) how do the specificity and sensitivity of these methods compare with one another? to answer these questions, we used 16s rrna tagged-pyrosequencing to sequence directly from a pooled fec ...201122092388
influence of quorum sensing signal molecules on biofilm formation in proteus mirabilis o18.the influence of basis of quorum sensing molecules on proteus strains is much less known as compared to pseudomonas or escherichia. we have previously shown that a series of acylated homoserine lactones (acyl-hsl) does not influence the ureolytic, proteolytic, or hemolytic abilities, and that the swarming motility of proteus mirabilis rods is strain specific. the aim of the presented study was to find out if the presence of a series of acyl-hsl influences biofilm formation of p. mirabilis labora ...201122198843
prevalence of viral, bacterial and parasitological diseases in rats and mice used in research environments in australasia over a 5-y period.viral, bacterial and parasitological infections in rats and mice used in biomedical research continue to occur despite improved housing and biosurveillance. the presence of disease in laboratory animals can lead to spurious results for research undertaken in universities, research institutes and the pharmaceutical industry. here the authors report the results of serological, microbiological, parasitological and molecular tests done on mice and rats from australasia submitted to a rodent health m ...201122012194
the new variant of salmonella genomic island 1 (sgi1-v) from a proteus mirabilis french clinical isolate harbours blaveb-6 and qnra1 in the multiple antibiotic resistance region.the clinical strain of proteus mirabilis vb1248 isolated from a blood culture in august 2009 was multiresistant (i.e. resistant to β-lactams, fluoroquinolones, aminoglycosides and sulphonamides). we searched for the presence of a salmonella genomic island 1 (sgi1).201121846670
diagnosis and management of extensive vascular malformations of the lower limb: part i. clinical diagnosis.there is significant confusion in the literature when describing vascular anomalies, and vascular malformations are often misnamed or incorrectly classified. part i of this two-part series on the diagnosis and management of extensive vascular malformations of the lower limbs will discuss the dermatologist's role in the diagnosis of these lesions. at least nine types of vascular malformations with specific clinical and radiologic characteristics must be distinguished in the lower limbs: klippel-t ...201122000870
antibacterial activities of 4-substituted-2-[(e)-{(1s,2r)/(1r,2s)-1-hydroxy-1-phenylpropan-2-ylimino}methyl]phenol.a series of norephedrine-based schiff bases (1a-6a and 1b-6b) were synthesized by reacting substituted salicylaldehydes with d-norephedrine or l-norephedrine. the structure of these compounds was confirmed by elemental analyses and spectroscopic techniques. the molecular structures of 5a and 6a have been determined by x-ray crystallography, which revealed that the compounds are in the oxoamino form, with bent intramolecular n-h···o (n···o ≈ 2.58 å) hydrogen bonds and that they are associated in ...201122098701
Use of a DNA microarray for detection and identification of bacterial pathogens associated with fishery products.We established a microarray for the simultaneous detection and identification of diverse putative pathogens often associated with fishery products by targeting specific genes of Listeria monocytogenes, Salmonella, Shigella, Staphylococcus aureus, Streptococcus pyogenes, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, and Yersinia enterocolitica and the 16S-23S rRNA gene internal transcribed spacer (ITS) region of Proteus mirabilis and Proteus vulgaris. The microarray contained 26 sp ...201121965411
multiple origins of endosymbiosis within the enterobacteriaceae (gamma-proteobacteria): convergence of complex phylogenetic approaches.abstract: background: the bacterial family enterobacteriaceae gave rise to a variety of symbiotic forms, from the loosely associated commensals, often designated as secondary (s) symbionts, to obligate mutualists, called primary (p) symbionts. determination of the evolutionary processes behind this phenomenon has long been hampered by the unreliability of phylogenetic reconstructions within this group of bacteria. the main reasons have been the absence of sufficient data, the highly derived nat ...201122201529
Impact of adopting minimum inhibitory concentration as the determinant of susceptibility to cephalosporins and carbapenems in multi-drug resistant Enterobacteriaceae.The Clinical and Laboratory Standards Institute has recommended that Enterobacteriaceae susceptibility to most cephalosporins and carbapenems be reported according to minimum inhibitory concentration (MIC) alone. We analyzed our record of multi-drug resistant Enterobacteriaceae to assess the impact of these changes. We compared susceptibilities of ceftriaxone-resistant Enterobacteriaceae when using the 2009 and new 2010 MIC standards. Vitek2® (BioMerieux), was used to assess the changes in susce ...201121912922
urinary pathogens and drug susceptibility patterns of urinary tract infections among antenatal clinic attendees in ibadan, nigeria.aim:  to determine the bacterial agents involved in urinary tract infections in pregnant women and their antibiotic susceptibility patterns in ibadan, nigeria. methods:  all consenting subjects who attended antenatal clinics of the university college hospital and adeoyo maternity hospital, ibadan, nigeria, from 1 april 2007 and 30 march 2009 were interviewed to obtain demographic and pregnancy health data. mid-stream urine samples obtained were processed by standard methods. confirmed bacterial ...201121917070
Microbiology of the chronic suppurative otitis media.The aim of the study was to determine aetiological agents of chronic suppurative inflammation of the middle ear and their susceptibility to antibiotics, which could lead to better therapeutic decisions and consequently avoidance of appearance of resistance to specific antibiotics. Most frequently isolated agents were Staphylococcus aureus (30.6%), Pseudomonas aeruginosa (22.4%), Klebsiella spp. (10.6%) and Proteus spp. (7.1%). None of the isolates were resistant to ciprofloxacin. High sensitivit ...201121849954
predictors of surgical site infections of the abdomen in lagos, nigeria.the issue of nosocomial infection constitute a significant burden on any health care facility particularly in resource constrained centers of many developing countries.201121913509
Participation of mast cells in chronic otitis media.In the pathogenesis of chronic otitis media (COM), much attention is paid to the molecular mechanisms of local inflammatory reactions in which mast cells (MCs) may be involved due to their role not only in allergic but also inflammatory processes. The aim of this study was to assess the density of mast cells in chronic otitis media in relationship to different clinical courses of COM, bacterial infections and types of disease. The MCs expression was measured immunohistochemically in paraffin-emb ...201122038229
dissemination of the rmtb gene carried on incf and incn plasmids among enterobacteriaceae in a pig farm and its investigate the prevalence and characterization of 16s rrna methylase-producing bacteria in a pig farm and its environment in east china.201121852287
description and plasmid characterization of qnrd determinants in proteus mirabilis and morganella morganii.clin microbiol infect abstract: we investigated the presence of qnrc and qnrd among 756 non-replicate enterobacteriaceae isolated in italy, selected for being non-susceptible to fluoroquinolones and/or resistant to third-generation cephalosporins. four proteus mirabilis and one morganella morganii (0.66% of the total) presented a qnrd gene, located in a 2687-base-pair plasmid that was entirely sequenced. the plasmid is un-typable, and contains no known coding region other than qnrd. that the qnr ...201122192340
chromosomal integration and location on inct plasmids of the blactx-m-2 gene in proteus mirabilis clinical isolates.analysis of five ctx-m-2-producing proteus mirabilis isolates in japan demonstrated that bla(ctx-m-2) was located on the chromosome in four isolates and on inct plasmids in three isolates, including two isolates that also carried the gene on the chromosome. in all four isolates with chromosomal bla(ctx-m-2), isecp1 was responsible for the integration of the gene into the chromosome. three different sites in the p. mirabilis genomic sequence were utilized as integration sites.201122106217
phytochemical constituents and antibacterial efficacy of the flowers of peltophorum pterocarpum (dc.) baker ex investigate the preliminary phytochemistry and antibacterial activity of the flower extract of peltophorum pterocarpum .201121967698
[evaluation of chromogenic medium uriselect4 in urine culture].we evaluated the performance and the cost of chromogenic medium uriselect4 agar with regard to the standard medium for the detection and identification of urinary tract pathogens. a total of 503 clinical urine specimens containing leucocytes greater or equal to 104/ml were analysed prospectively, in parallel by two different persons on blood agar (gs) and uriselect4 according to the manufacturers' instructions. of the 503 urine specimens tested, 210 gave a positive culture on uriselect4 versus 1 ...201122008133
Deciphering simultaneous bioelectricity generation and dye decolorization using Proteus hauseri.This first-attempt study disclosed how and why electron-shuttling mediators were capable to stimulate bioelectricity-generating capabilities of dye-bearing microbial fuel cells (MFCs) using Proteus hauseri. Due to significant biotoxicity of 4-aminophenol (4AP) and the absence of electron-mediating potential of 3AP, only 2AP among all isomers could work as an exogenous mediator to stimulate bioelectricity generation of P. hauseri. Dye toxicity to cells on anodic biofilm in MFCs apparently affecte ...201122178022
development of a specific polymerase chain reaction assay for the detection of basidiobolus.the aethiology of chronic diarrhoea is complex in humans and animals. it is always necessary to evaluate a list of differential diagnosis, including bacteria, protozoa and fungi. basidiobolomycosis is a fungal disease reported sporadically worldwide, mainly caused by b. ranarum, a frequent organism found in soil or in the intestine and skin of lizards and frogs. it is an opportunistic infection characterized by granulomatous lesions in the subcutaneous tissues but also in the intestine wall in h ...201122075784
Comparative study of antimicrobial activity and phytochemical analysis of methanolic and aqueous extracts of the fruit of Emblica officinalis against pathogenic bacteria.To investigate antimicrobial potential of aqueous (infusions, decoctions) and methanlic extracts (1:2 and 1:5 concentrations) of Emblica officinalis (amla) against seven pathogenic bacteria namely Staphylococcs aureus, Staphylococcus saprophyticus, Escherichia coli, Enterococcus faecalis, Enterococcus cloacae, Proteus vulgaris and Klebsiella pneumoniae.201121977871
antimicrobial activity of two essential oils.the aim of the study was to evaluate the antimicrobial activity of essential oils in vitro for possible application to reduce the content of microorganisms in the air of animal houses. the essential oils of cymbopogon citrarus l. and malaleuca alternifolia l. were screened against bacteria staphylococcus aureus, enterococcus faecium, pseudomonas aeruginosa, escherichia coli, proteus mirabilis and yeast candida albicans. the minimal inhibitory concentration of the active essential oils was tested ...201121739934
a new class of 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazine analogues with antimicrobial/antimycobacterial activity.this study presents the synthesis and in vitro pharmacological evaluations of novel 2-(4-cyanophenyl amino)-4-(6-bromo-4-quinolinyloxy)-6-piperazinyl (piperidinyl)-1,3,5-triazines. the title compounds were assayed for their in vitro antimicrobial activity against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria) and four fungi (aspergillus niger, aspergillus fumigatus, a ...201121740100
surface microbial consortia from livarot, a french smear-ripened cheese.the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ...201121815832
evaluation of efficacy of topical povidone-iodine and different types of fluoroquinolones in the sterilization of bacterial flora on the conjunctiva.abstract purpose: to compare the sterilization effectivity of topical povidone-iodine, ciprofloxacin, and ofloxacin on the conjunctival bacterial flora. methods: one hundred sixty-four eyes of 164 patients scheduled for cataract surgery were prospectively analyzed. conjunctival cultures were obtained 1 day before the surgery without any topical application in all patients. conjunctival flora was examined in 53 eyes of 53 patients (group i) 15ôçëmin after 5% topical povidone-iodine application on ...201121834670
antimicrobial resistance patterns of uropathogens among children in istanbul, turkey.urinary tract infections are a common cause of end-stage renal disease in turkey. this prospective study investigated the antibiotic resistance patterns of uropathogens in order to recommend appropriate therapeutic protocols for children with urinary tract infections in istanbul, turkey. between october 2007 and october 2008, children presenting with a first episode of urinary tract infection to a pediatric outpatient clinic were enrolled in the study. urine samples were cultured, and antimicrob ...201121710858
acidic environments induce differentiation of proteus mirabilis into swarmer morphotypes.although swarmer morphotypes of proteus mirabilis have long been considered to result from surfaced-induced differentiation, the present findings show that, in broth medium containing urea, acidic conditions transform some swimmer cells into elongated swarmer cells. this study has also demonstrates that p. mirabilis cells grown in acidic broth medium containing urea enhance virulence factors such as flagella production and cytotoxicity to human bladder carcinoma cell line t24, though no signific ...201121707738
[russian results of the international epidemiological study of clinical aspects and resistance to antimicrobial medicines of cystitis uropathogens in females (aresc): significance of empiric therapy].a total of 656 female patients from russia aged between 18 to 65 years with uncomplicated cystitis entered the international aresc trial, of them 647 were eligible for final analysis. positive cultural urine tests ( > 10(4) cfu/ml) were in 419 (64.7%) patients, 393 (93.8%) patients had monoinfection. the central laboratory of genoa (to which the isolated samples were sent) has tested 416 uropathogens from 399 patients. the following pathogens were isolated: e. coli (72.6%), enterococcus (7.0%), ...201121815455
detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (sift-ms).selected ion flow tube-mass spectrometry has been used to measure the volatile compounds occurring in the headspace of urine samples inoculated with common urinary tract infection (uti)-causing microbes escherichia coli, proteus vulgaris, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, enterococcus faecalis, or candida albicans. this technique has the potential to offer rapid and simple diagnosis of the causative agent of utis.201121741416
investigation of the antimicrobial activity of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.).the aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. material and methods. ethanol extract from the leaves of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.) was produced at the department of food technology, kaunas university of technology. the antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria s ...201121822040
Cerebriform Plantar Hyperplasia: The Clinico-pathological Hallmark of Proteus Syndrome. 201121681363
Macrodactyly with skin hypertrophy: a minimal form of the Proteus syndrome.The Proteus syndrome was described 1983 . It has asymmetric gigantism of the limbs, verrucous epidermal naevi, cerebriform enlargement of the plantar region, vascular malformations and neoplasms, as lipomas. It received this denomination after Proteus from the Greek mythology, who had the ability to change his form . A 15 year-old boy, reported a congenital hypertrophy with syndactily of the second and third right fingers . The second case is a 35 year-old man, who reported that since birth the ...201121738976
Trimethoprim/sulfametrole: evaluation of the available clinical and pharmacokinetic/pharmacodynamic evidence.Emergence of resistance to widely used trimethoprim/sulfamethoxazole (TMP/SMX) as well as common adverse events in human immunodeficiency virus (HIV)-infected patients casts interest on combinations of TMP with other sulfonamides. Sulfametrole (SMT) combined with TMP could provide a choice for difficult-to-treat infections, particularly when administered intravenously. The objective of this review was to evaluate the available clinical and pharmacokinetic/pharmacodynamic (PK/PD) evidence regardi ...201121741802
relationship between chemical structure and biological activity of alkali metal o-, m- and p-anisates. ft-ir and microbiological this work we investigated relationship between molecular structure of alkali metal o-, m-, p-anisate molecules and their antimicrobial activity. for this purpose ft-ir spectra for lithium, sodium, potassium, rubidium and caesium anisates in solid state and solution were recorded, assigned and analysed. microbial activity of studied compounds was tested against escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and proteus vulgaris. in order to evaluate the dependency between chem ...201121852187
the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds.the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ...201122182219
vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ...201121917010
Development of an antibiogram in a long-term care facility.To create an antibiogram-a profile of an organism's susceptibility/resistance to a panel of antibiotics- for a long-term care facility to assess the prevalence of resistance of bacteria present at the facility.201122079792
Antimicrobial susceptibility of Gram-negative organisms from intraabdominal infections and evolution of isolates with extended spectrum ß-lactamases in the SMART study in Spain (2002-2010).Introduction. The SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study records the antimicrobial susceptibility of Gram-negative bacilli obtain from intraabdominal infections with special focus in isolates with extended spectrum ß-lactamases (ESBLs). Material and Methods. The antimicrobial susceptibility of 8,869 isolates was analyzed by microdilution during the SMART study performed in Spain from 2002 to 2010. Isolates were recovered in 16 centres. Results. Escherichi ...201122173194
Origins of the elephant man: mosaic somatic mutations cause Proteus syndrome. 201122035467
[Synthesis and antimicrobial evaluation of coumarin-based benzotriazoles and their synergistic effects with chloromycin and fluconazole].A series of new coumarin-based benzotriazole derivatives were successfully synthesized via a multi-step sequence of cyclization, etherification and N-alkylation, and were confirmed by 1H NMR, IR, MS spectra as well as elemental analyses. All these synthesized coumarin compounds were evaluated for in vitro antimicrobial activities against four Gram-positive bacteria, four Gram-negative bacteria and three fungi by two fold serial dilution technique. The bioactive assay showed that all these prepar ...201122010349
Plasmid-mediated transfer of the bla(NDM-1) gene in Gram-negative rods.The latest threat of multidrug-resistant Gram-negative bacteria corresponds to the emergence of carbapenemase NDM-1 (New Delhi metallo-ß-lactamase) producers, mostly in Enterobacteriacae. Five bla(NDM) (-1) -positive plasmids of different incompatibility groups (IncL/M, FII, A/C and two untypeable plasmids) from clinical Enterobacteriaceae were evaluated for conjugation properties and host specificity. Successful conjugative transfers were obtained using all tested enterobacterial species as rec ...201122092811
clinical characteristics and outcomes of patients with extended-spectrum β-lactamase-producing bacteremias in the emergency department.extended-spectrum β-lactamase (esbl)-producing bacteria have been spreading from hospitals to communities. despite this, there are limited emergency department (ed) patient-based studies about these bacteremias. a retrospective matched case-control study with a ratio of 1:3 was conducted at a university hospital. the case group consisted of patients aged >16 years with esbl-producing bacteremias in the ed. patients matched for age and sex with non-esbl-producing bacteremias were sampled as the c ...201122033790
Bactericidal properties of pradofloxacin against veterinary pathogens.Pradofloxacin is a new veterinary 8-cyano-fluoroquinolone developed for use against bacterial infections in dogs and cats involving both aerobic and anaerobic bacteria. The minimal bactericidal concentrations have been determined against clinical isolates of Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus canis, Proteus spp., Fusobacterium spp., Porphyromonas gingivalis and Prevotella species. A subset of these species was selected, ...201122209121
nosocomial infection and characterization of extended-spectrum β-lactamases-producing enterobacteriaceae in northeast brazil.extended spectrum β-lactamases (esbls) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide.201121860889
macroscopic behavior of systems with an axial dynamic preferred direction.we present the derivation of the macroscopic equations for systems with an axial dynamic preferred direction. in addition to the usual hydrodynamic variables, we introduce the time derivative of the local preferred direction as a new variable and discuss its macroscopic consequences including new cross-coupling terms. such an approach is expected to be useful for a number of systems for which orientational degrees of freedom are important including, for example, the formation of dynamic macrosco ...201122120542
Acinetobacter baumannii is Able to Gain and Maintain a Plasmid Harbouring In35 Found in Enterobacteriaceae Isolates From Argentina.The aim of this study was to determine the presence of bla (CTX-M-2) in our A. baumannii population and their putative role as an alternative mechanism of resistance to third-generation cephalosporins in this species. The bla (CTX-M-2) gene is widespread among the Enterobacteriaceae isolates from our country; however, it was not found in 76 isolates A. baumannii non-epidemiologically related clinical isolates resistant to third-generation cephalosporins ...201122119898
[correlation between histomorphometric changes and the type of aerobic bacteria isolated in chronic suppurative otitis media].bacterial flora is a very important factor in pathogenesis of chronic suppurative otitis media (csom) and significantly influences the type and intensity of osteolytic process. there are few histomorphometric investigations of middle ear mucosa in chronic otitis. the aim of this study was to identify aerobic bacteria responsible for chronic suppurative otitis media as well as their association with histomorphometric changes of middle ear mucosa.201121425618
correlation between antibiotic consumption and resistance of gram-negative bacteria causing healthcare-associated infections at a university hospital in taiwan from 2000 to 2009.this study investigated the correlation between antibiotic consumption and antimicrobial resistance in gram-negative bacteria causing healthcare-associated infections at a university hospital in taiwan from 2000 to 2009.201121436153
epidemiology of ciprofloxacin resistance and its relationship to extended-spectrum +¦-lactamase production in proteus mirabilis bacteremia.we evaluated the clinical features of ciprofloxacin-resistant proteus mirabilis bacteremia and risk factors for ciprofloxacin resistance.201121437168
re: strategy to control catheter encrustation with citrated drinks: a randomized crossover study. a. khan, f. housami, r. melotti, a. timoney and d. stickler. j urol 2010; 183: 1390-1394. 201121439588
antimicrobial activity of organotin(iv) complexes with the ligand benzil bis(benzoylhydrazone) and 4,4'-bipyridyl as coligand.several methyltin(iv) and butyltin(iv) complexes with the ligand benzil bis(benzoylhydrazone) and 4,4'-bipyridyl as coligand were synthesised and characterized by elemental analysis and by ir, (1)h, (13)c and (119)sn nmr spectroscopies. some of them were also analyzed using single crystal x-ray diffraction. the title compounds were evaluated for their in vitro antimicrobial properties. all buthyltin complexes showed significant inhibition of gram positive bacteria, resulting bacillus subtilis, s ...201121443849
[practice management of chronic bacterial prostatitis with levofloxacin].the aim of this prospective observational study was to present relevant data on the efficacy and safety of levofloxacin in the treatment of chronic bacterial prostatitis (nih-ii) in the daily clinical routine of urologists in their practices. in total, 243 patients were treated, including 62 patients with a microbiologically confirmed diagnosis -based on expressed prostatic secretion (eps). the current evaluation is based on these patients. their median age was 53 years. the patients were treate ...201121445824
extended-spectrum beta-lactamase orthopedic wound infections in nigeria.extended-spectrum beta-lactamase (esbl)-producing gram-negative bacteria are emerging and impacting significantly on the management of patients and hospital costs. besides, they are not being routinely sought after in diagnostic laboratories thus contributing to treatment failure.201121887050
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