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convenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory.although transmissible metallo-beta-lactamases (mbls) are a serious threat to beta-lactam antibiotic therapy, the clsi currently does not recommend testing methods for the detection of mbls. the aim of this study was to evaluate the capability of double-disk tests (ddts) by using disks containing a combination of the chelators 2-mercaptopropionic acid (mpa) and tris-edta (te) to detect mbls. sixteen isolates (4 acinetobacter baumannii isolates, 6 pseudomonas aeruginosa isolates, 1 serratia marce ...200717596358
structure of a new ribitol teichoic acid-like o-polysaccharide of a serologically separate proteus vulgaris strain, tg 276-1, classified into a new proteus serogroup o53.an unusual ribitol teichoic acid-like o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide from a previously non-classified proteus vulgaris strain tg 276-1. structural studies using chemical analyses and 2d (1)h and (13)c nmr spectroscopy showed that the polysaccharide is a zwitterionic polymer with a repeating unit containing 2-acetamido-4-amino-2,4,6-trideoxy-d-galactose (d-fucnac4n) and two d-ribitol phosphate (d-rib-ol-5-p) residues and having the following struc ...200717601512
new aspects of membrane dynamics of amoeba proteus contractile vacuole revealed by vital staining with fm 4-64.the contractile vacuole (cv) cycle of amoeba proteus has been studied by phase contrast and electron microscopy. however, the understanding of membrane dynamics in this cycle is still poor. in this study, we used live imaging by fluorescence microscopy to obtain new insights. we succeeded in staining the cv with a styryl dye, fm 4-64 (n-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium dibromide), and obtained the following results. (1) the cv membrane was directly s ...200717602276
actin dynamics in amoeba proteus motility.we studied the distribution of the endogenous arp2/3 complex in amoeba proteus and visualised the ratio of filamentous (f-actin) to total actin in living cells. the presented results show that in the highly motile amoeba proteus, arp2/3 complex-dependent actin polymerisation is involved in the formation of the branching network of the contractile layer, adhesive structures, and perinuclear cytoskeleton. the aggregation of the arp2/3 complex in the cortical network, with the exception of the uroi ...200717602277
synthesis and structure of silver complexes with nicotinate-type ligands having antibacterial activities against clinically isolated antibiotic resistant pathogens.the synthesis and low-temperature x-ray crystal structures of five new silver complexes, [ag(2)-mu-o,o'(2-aminonicotinium)(2)(no(3))(2)](n) (7), [ag(isonicotinamide)(2)-mu-o,o'(no(3))](2) (8), [ag(ethyl nicotinate)(2)](no(3)) (9), [ag(ethyl isonicotinate)(2)(no(3))] (10), and [ag(methyl isonicotinate)(2)(h(2)o)](no(3)) (11), are presented and fully characterized by spectral and elemental analysis. the antimicrobial activities of these complexes were screened using 12 different clinical isolates ...200717602550
in vitro activity of cefadroxil, cephalexin, cefatrizine and cefpirome in presence of essential and trace elements.evidences supporting the introduction of metallic elements in several biological processes are rapidly accumulating. likewise, many drugs possess modified toxicological and pharmacological properties when in the form of metal complexes. in order to ascertain the role of various essential and trace element complexation on the antibacterial activity of various cephalosporins, the synergistic or antagonistic behavior of cefadroxil, cephalexin, cefatrizine and cefpirome in presence of essential and ...200717604254
interspecies spread of ctx-m-32 extended-spectrum beta-lactamase and the role of the insertion sequence is1 in down-regulating bla ctx-m gene expression.to characterize the extended-spectrum beta-lactamases (esbls) as well as their genetic environment in different isolates of enterobacteriaceae from a patient with repeated urinary tract infections.200717332005
microbiology of diabetic foot infections in a teaching hospital in malaysia: a retrospective study of 194 cases.diabetes mellitus is a progressive disease with chronic complications. foot infections are a major complication of diabetes and eventually lead to development of gangrene and lower extremity amputation. the microbiological characteristics of diabetic foot infections have not been extensively studied in malaysia. this study investigated the microbiology of diabetic foot infections and their resistance to antibiotics in patients with diabetic foot infections treated at university of malaya medical ...200717332905
digestive tract microbiota in healthy volunteers.the aim of this study was to standardize the methods of sample collection of mucus from the digestive tract and to determine the microbiota in healthy volunteers from brazil, collecting samples from the mouth, esophagus, stomach, duodenum, jejunum, ileum, colon, and rectum.200717334549
amoeba proteus displays a walking form of locomotion.this report deals with observations on the directional locomotion of amoeba before and after fixation and scanning electron microscopy. the study was aimed at visualization of the stepwise events of directional movements. after the analysis of the data it is proposed that the amoeba undergoes a sequence of movement events that can be defined as a walking form of locomotion.200717336550
[incidence and clinical characteristics of symptomatic urinary infections in a geriatric hospital].objectives and settings: the authors had for aim to study the incidence of symptomatic urinary infections (suti) in elderly patients, to describe their clinical and microbiologic characteristics and first-line treatment in a geriatric hospital with 902 beds: 124 in acute care (acf), 293 in rehabilitation and intermediate-care (ricf), and 485 in long-term-care-facilities (ltcf).200717337143
linear cowden nevus: a new distinct epidermal nevus.within the group of epidermal nevi, a so far nameless disorder is described under the term "linear cowden nevus". this non-organoid epidermal nevus is caused by loss of heterozygosity, occurring at an early developmental stage in an embryo with a germline pten mutation, giving rise to cowden disease. hence, linear cowden nevus can be categorized as a characteristic feature of type 2 segmental cowden disease. until now, several authors had mistaken this epidermal nevus as a manifestation of prote ...200717337396
mild and delayed-onset proteus syndrome. 200717337412
antibacterial activities of emblica officinalis and coriandrum sativum against gram negative urinary pathogens.present investigation is focused on antibacterial potential of aqueous infusions and aqueous decoctions of emblica officinalis (amla) and coriandrum sativum (coriander) against 345 bacterial isolates belonging to 6 different genera of gram negative bacterial population isolated from urine specimens by employing well diffusion technique. aqueous infusion and decoction of emblica officinalis exhibited potent antibacterial activity against escherichia coli (270), klebsiella pneumoniae (51), k. ozae ...200717337425
surveillance of antimicrobial susceptibility of urinary pathogens in a tertiary care hospital. 200717342301
surveillance of extended-spectrum beta-lactamase-producing bacteria and routine use of contact isolation: experience from a three-year period.the usefulness and applicability of isolation precautions were questioned for extended-spectrum beta-lactamase (esbl)-producing strains of enterobacteriaceae in the endemic setting. we performed a surveillance programme for esbl-positive organisms and the infection control management of patients colonized or infected with these organisms. between 1 january 2002 and 31 december 2004, a total of 147 cases of esbl-producing strains of escherichia coli, klebsiella pneumoniae and proteus mirabilis fr ...200717350720
characteristics of motive force derived from trajectory analysis of amoeba proteus.we used a monochromatic charge-coupled-device camera to observe the migration behavior of amoeba proteus every 5 s over a time course of 10000 s in order to investigate the characteristics of its centroid movement (cell velocity) over the long term. fourier transformation of the time series of the cell velocity revealed that its power spectrum exhibits a lorentz type profile with a relaxation time of a few hundred seconds. moreover, some sharp peaks were found in the power spectrum, where the ra ...200717351734
does water activity rule p. mirabilis periodic swarming? i. biochemical and functional properties of the extracellular matrix.the dynamics of bacterial colonies is complex in nature because it correlates the behavior of numerous individual cells in space and time and is characterized by emergent properties such as virulence or antibiotics resistance. because there is no clear-cut evidence that periodic swarming of p. mirabilis colonies is ruled by chemical triggers responsible for cell-to-cell signaling in most of the biofilms, we propose that the observed periodicity relies on the colony's global properties. hence, th ...200717355120
does water activity rule p. mirabilis periodic swarming? ii. viscoelasticity and water balance during swarming.following the analysis of the biochemical and functional properties of the p. mirabilis extra cellular matrix performed in the first part of this study, the viscoelasticity of an actively growing colony was investigated in relation to water activity. the results demonstrate that the p. mirabilis colony exhibits a marked viscoelastic character likely due to both cell rafts and exoproduct h-bond networks. besides, the water loss by evaporation during migration has been measured, whereas the experi ...200717355121
pericardial tamponade masquerading as septic shock.a 53-year-old man with steroid dependent rheumatoid arthritis presented with fever and serous articular drainage. oral antibiotics were initially prescribed. subsequent hemodynamic instability was attributed to septic shock. further evaluation revealed a pericardial effusion with tamponade. pericardiocentesis of purulent fluid promptly corrected the hypotension. proteus mirabilis was later isolated from both the infected joint and the pericardial fluid. this is the first report of combined prote ...200717356998
[ocular manifestations in proteus syndrome].case report: congenital disfiguring malformations are rare and usually have a multifactorial aetiology. here we report on the ocular manifestations seen in a patient with proteus syndrome. the retina showed retinal dysgenesia, retinal pigmentary abnormalities and optic nerve hypoplasia. other abnormalities included strabismus and high myopia. discussion: proteus syndrome is a complex hamartomatous disorder defined by local overgrowth, subcutaneous tumours and various bone, cutaneous and/or vascu ...200717357896
microfluidic chip analysis of outer membrane proteins responsible for serological cross-reaction between three gram-negative bacteria: proteus morganii o34, escherichia coli o111 and salmonella adelaide o35.bacterial strains have complex and individual antigenic structure, which provides basis for their serological identification. however, serological cross-reaction may occur when antibodies against a certain strain recognize other strains too. the molecular basis of this phenomenon is the expression of similar or identical antigenic epitopes on the surface of different bacterial cells. such cross-reactions might harden the serological diagnosis of pathogenic bacteria. but it can be also advantageo ...200717362972
germa-gamma-lactones as novel inhibitors of bacterial urease activity.organogermanium compounds have been used as pharmacological agents. however, very few reports are available on the synthesis and antibacterial activities of lactones containing organogermaniums. the purpose of the present investigation was to determine the effects of different lactone-substituted organogermaniums on bacterial growth and their urease activity. we report synthesis of 12 germa-gamma-lactones (gel) and their antimicrobial activities against several bacterial pathogens. antibacterial ...200717367756
detection of extended-spectrum beta-lactamases among enterobacteriaceae by use of semiautomated microbiology systems and manual detection procedures.three commercially available microbiology identification and susceptibility testing systems were compared with regard to their ability to detect extended-spectrum beta-lactamase (esbl) production in enterobacteriaceae, i.e., the phoenix automated microbiology system (bd diagnostic systems, sparks, md), the vitek 2 system (biomérieux, marcy l'etoile, france), and the microscan walkaway-96 system (dade behring, inc., west sacramento, ca), using routine testing panels. one hundred fifty putative es ...200717287329
potential integration sites of the salmonella genomic island 1 in proteus mirabilis and other bacteria. 200717293370
spectroscopic description of an unusual protonated ferryl species in the catalase from proteus mirabilis and density functional theory calculations on related models. consequences for the ferryl protonation state in catalase, peroxidase and chloroperoxidase.the catalase from proteus mirabilis peroxide-resistant bacteria is one of the most efficient heme-containing catalases. it forms a relatively stable compound ii. we were able to prepare samples of compound ii from p. mirabilis catalase enriched in (57)fe and to study them by spectroscopic methods. two different forms of compound ii, namely, low-ph compound ii (lph ii) and high-ph compound ii (hph ii), have been characterized by mössbauer, extended x-ray absorption fine structure (exafs) and uv-v ...200717237942
the first structure of a cold-active catalase from vibrio salmonicida at 1.96 a reveals structural aspects of cold adaptation.the cold-adapted catalase from the fish-pathogenic bacterium vibrio salmonicida (vsc) has recently been characterized and shown to be two times more catalytically efficient compared with catalase from the mesophilic human pathogen proteus mirabilis [pmc; lorentzen et al. (2006), extremophiles, 10, 427-440]. vsc is also less temperature-stable, with a half-life of 5 min at 333 k compared with 50 min for pmc. this was the background for solving the crystal structure of the cold-adapted vsc to 1.96 ...200717242507
rhomboid protease aara mediates quorum-sensing in providencia stuartii by activating tata of the twin-arginine translocase.the providencia stuartii aara protein is a member of the rhomboid family of intramembrane serine proteases and is required for the production of an unknown quorum-sensing molecule. in a screen to identify rhomboid-encoding genes from proteus mirabilis, tata was identified as a multicopy suppressor and restored extracellular signal production as well as complementing all other phenotypes of a prov. stuartii aara mutant. tata is a component of the twin-arginine translocase (tat) protein secretion ...200717215357
structure of proteus mirabilis biofilms grown in artificial urine and standard laboratory media.proteus mirabilis is a urinary pathogen that can differentiate from a swimmer cell into a swarmer cell morphotype and can form biofilms on the surfaces of urinary catheters. these biofilms block these catheters due to crystals trapped within these structures. the effect of encrustation on biofilm formation and structure has not been studied using confocal scanning laser microscopy (cslm). therefore, a comparison of biofilm structure in artificial urine (au) and laboratory media was undertaken. w ...200717250761
emerging bacterial pathogens: escherichia coli, enterobacter aerogenes and proteus mirabilis clinical isolates harbouring the same transferable plasmid coding for metallo-beta-lactamase vim-1 in greece. 200717255145
synthesis of pseudopeptides based l-tryptophan as a potential antimicrobial agent.four compounds named l-btrppa, l-trp-o-pa, l-trp-m-pa and l-trp-p-pa, pseudopeptides constructed from pyridine and tryptophan units, were synthesized and tested against the gram-positive, gram-negative strains of bacteria and human pathogenic fungi. l-trp-o-pa proved to be a broad-spectrum antimicrobial agent, showing a significant inhibition of the growth of gram-positive bacteria (staphylococcus aureus, methicillin-resistant s. aureus, bacillus subtilis, micrococcus luteus), and pathogenic fun ...200717257839
[community-acquired urinary tract infections in 15 to 65 years old female patients in france. susceptibility of e. coli according to history: aforcopi-bio network 2003].a multicenter study was implemented in order to determine the distribution and antibiotic susceptibility patterns of strains isolated from 15 to 65 year old female patients with community-acquired urinary tract infections.200717258415
rheumatoid arthritis is linked to proteus--the evidence.rheumatoid arthritis (ra) is a chronic inflammatory arthritic and potentially disabling condition, mainly affecting women of middle age and having characteristic clinical features. various microbial agents were implicated in the causation of ra. extensive literature based on the results of various genetic, microbiological, molecular, and immunological studies carried out by independent research groups supports the role of proteus mirabilis bacteria in the etiopathogenesis of ra. new diagnostic m ...200717206398
use of boronic acid disk methods to detect the combined expression of plasmid-mediated ampc beta-lactamases and extended-spectrum beta-lactamases in clinical isolates of klebsiella spp., salmonella spp., and proteus mirabilis.a study using boronic acid (ba), an ampc enzyme inhibitor, was designed to detect the combined expression of plasmid-mediated ampc beta-lactamases (pampcs) and extended-spectrum beta-lactamases (esbls) in bacterial isolates naturally lacking chromosomal ampc genes. a total of 122 klebsiella spp., salmonella spp., and proteus mirabilis isolates producing or nonproducing pampcs and/or esbls were analyzed. detection of genes encoding esbls and ampcs was confirmed by polymerase chain reaction (pcr) ...200717174510
infection of a congenital epidural dermoid cyst secondary to lumbar puncture. 200717179297
proteus bacteriuria is associated with significant morbidity in spinal cord injury.retrospective chart review.200717179975
structure of the o-polysaccharide and serological cross-reactivity of the lipopolysaccharide of providencia alcalifaciens o32 containing n-acetylisomuramic acid.the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o32 and studied by sugar and methylation analyses, solvolysis with triflic acid, 1h and 13c nmr spectroscopy, including two-dimensional 1h,1h cosy, tocsy, roesy, h-detected 1h,13c hsqc and hmbc experiments. it was found that the polysaccharide has a branched tetrasaccharide repeating unit containing 2-acetamido-3-o-[(s)-1-carboxyethyl]-2-deoxy-d-glucose (d-glcnac3slac, n-acetylisomur ...200717182016
helicobacter pylori: an underestimated factor in acute anterior uveitis and spondyloarthropathies?acute anterior uveitis (aau) is the most common form of intraocular inflammation, but its aetiology is still unclear. fifty percent of aau patients are hla-b27-positive, and half of these also have spondyloarthropathies (spa). numerous serological studies have shown elevated levels of serum antibodies to various gram-negative bacteria in hla-b27-positive aau and spa patients. antigenic similarities between these bacteria and host components (hla-b27) have already been shown. still, the mechanism ...200717183194
both sulfate-reducing bacteria and enterobacteriaceae take part in marine biocorrosion of carbon steel.in order to evaluate the part played in biocorrosion by microbial groups other than sulfate-reducing bacteria (srb), we characterized the phylogenetic diversity of a corrosive marine biofilm attached to a harbour pile structure as well as to carbon steel surfaces (coupons) immersed in seawater for increasing time periods (1 and 8 months). we thus experimentally checked corroding abilities of defined species mixtures.200717184331
role of ser11 in the stabilization of the structure of ochrobactrum anthropi glutathione transferase.gsts (glutathione transferases) are a multifunctional group of enzymes, widely distributed and involved in cellular detoxification processes. in the xenobiotic-degrading bacterium ochrobactrum anthropi, gst is overexpressed in the presence of toxic concentrations of aromatic compounds such as 4-chlorophenol and atrazine. we have determined the crystal structure of the gst from o. anthropi (oagst) in complex with gsh. like other bacterial gsts, oagst belongs to the beta class and shows a similar ...200717223798
the xaxab genes encoding a new apoptotic toxin from the insect pathogen xenorhabdus nematophila are present in plant and human pathogens.xenorhabdus nematophila, a member of the enterobacteriaceae, kills many species of insects by strongly depressing the immune system and colonizing the entire body. a peptide cytotoxin has been purified from x. nematophila broth growth, and the cytolytic effect on insect immunocytes and hemolytic effect on mammalian red blood cells of this toxin have been described (ribeiro, c., vignes, m., and brehélin, m. (2003) j. biol. chem. 278, 3030-3039). we show here that this toxin, xenorhabdus alpha-xen ...200717229739
the effect of lipopolysaccharide from proteus mirabilis on the level of the stable end metabolic products of nitric oxide in blood platelets.nitric oxide ((*)no) plays an important role in a number of physiologic processes. evidence exists that (*)no, which stimulates soluble guanylate cyclase and enhances cyclic guanosine monophosphate (cgmp) levels, may inhibit platelet activation. in contrast, during platelet activation induced by different agonists, synthesis of (*)no in platelets occurs. in these studies, production of the stable end-products of (*)no-nitrite and nitrate (no(x)) in human platelets, stimulated by different doses ...200717160361
a comparison of the binding of secretory component to immunoglobulin a (iga) in human colostral s-iga1 and s-iga2.a detailed investigation of the binding of secretory component to immunoglobulin a (iga) in human secretory iga2 (s-iga2) was made possible by the development of a new method of purifying s-iga1, s-iga2 and free secretory component from human colostrum using thiophilic gel chromatography and chromatography on jacalin-agarose. sodium dodecyl sulphate-polyacrylamide gel electrophoresis of unreduced pure s-iga2 revealed that, unlike in s-iga1, a significant proportion of the secretory component was ...200717156102
antimicrobial resistance among uropathogens that cause community-acquired urinary tract infections in antananarivo, madagascar.urinary tract pathogens obtained from patients in madagascar are becoming increasingly resistant to commonly used antibiotics that are readily available at a low price. this poses a real problem for the treatment of community-acquired urinary tract infections (utis) in madagascar.200717138569
proteus mirabilis clinical isolate harbouring a new variant of salmonella genomic island 1 containing the multiple antibiotic resistance region.a clinical isolate of proteus mirabilis strain 18306, which displayed the multidrug resistance phenotype of salmonella genomic island 1 (sgi1), was examined for the presence of this island including its multiple antibiotic resistance genomic region.200717114173
a microbial diagnostic microarray technique for the sensitive detection and identification of pathogenic bacteria in a background of nonpathogens.a major challenge in microbial diagnostics is the parallel detection and identification of low-bundance pathogens within a complex microbial community. in addition, a high specificity providing robust, reliable identification at least at the species level is required. a microbial diagnostic microarray approach, using single nucleotide extension labeling with gyrb as the marker gene, was developed. we present a novel concept applying competitive oligonucleotide probes to improve the specificity o ...200717123456
increased serum iga and igm against lps of enterobacteria in chronic fatigue syndrome (cfs): indication for the involvement of gram-negative enterobacteria in the etiology of cfs and for the presence of an increased gut-intestinal permeability.there is now evidence that chronic fatigue syndrome (cfs) is accompanied by immune disorders and by increased oxidative stress. the present study has been designed in order to examine the serum concentrations of iga and igm to lps of gram-negative enterobacteria, i.e. hafnia alvei; pseudomonas aeruginosa, morganella morganii, proteus mirabilis, pseudomonas putida, citrobacter koseri, and klebsiella pneumoniae in cfs patients, patients with partial cfs and normal controls. we found that the preva ...200717007934
there is a low incidence of recurrent bacteriuria in painful bladder syndrome/interstitial cystitis patients followed longitudinally.the objective of this paper was to establish whether patients with confirmed painful bladder syndrome/interstitial cystitis (pbs/ic) presenting with symptoms of uti have actual bacteriuria vs a flare of their pbs/ic symptoms. one hundred and six (n = 106) consecutive female patients (mean age 39.8 +/- 14 years) with newly diagnosed ic were identified and followed longitudinally for 24 months. at the initial visit and at all subsequent visits, urinary specimens were obtained by sterile catheteriz ...200717036170
antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection.to compare the effectiveness of antimicrobial photodynamic therapy (pdt), standard endodontic treatment and the combined treatment to eliminate bacterial biofilms present in infected root canals.200717066481
are laboratory-based antibiograms reliable to guide the selection of empirical antimicrobial treatment in patients with hospital-acquired infections?antibiograms are often taken into account to define a rational selection of an empirical antimicrobial therapy for treating patients with hospital-acquired infections. in this study, we performed a paired comparison between the antibiogram constructed with laboratory-based data and that formed with data subjected to prior clinical validation.200717079239
in vitro activities of various piperacillin and sulbactam combinations against bacterial pathogens isolated from intensive care units in taiwan: smart 2004 programme data.we investigated the in vitro activity of various piperacillin and sulbactam combinations against gram-negative bacterial isolates from intensive care units (icus) in taiwan. antimicrobial susceptibility testing of 1030 bacterial isolates recovered from icus of nine major teaching hospitals was performed using the agar dilution method. sulbactam was added to piperacillin either at a fixed sulbactam concentration of 4 mg/l and 8 mg/l or at a piperacillin:sulbactam ratio of 2:1 and 4:1. piperacilli ...200716815690
three cases of xanthogranulomatous epididymitis caused by e. coli.xanthogranulomatous epididymitis is an uncommon but benign process, characterized by tissue destruction and the accumulation of abundant foamy macrophages mixed with lymphocytes and plasma cells. the pathogens generally found to cause xanthogranulomatous inflammation have been proteus and e. coli. we identified such a pathogen by sequencing divergent regions of 16s rrna, followed by pathogen-specific-pcr using alr, ipah, ial, and iuc primer pairs in dna microdissected from an h&e stained slide. ...200716762415
biodegradation and decolourization of anaerobically treated distillery spent wash by a novel bacterial consortium.the aim of this study was to isolate microorganisms capable of decolourizing and degrading anaerobically treated distillery spent wash. a bacterial consortium dmc comprising of three bacterial cultures was selected on the basis of rapid effluent decolourization and degradation, which exhibited 67 +/- 2% decolourization within 24 h and 51 +/- 2% chemical oxygen demand reduction within 72 h when incubated at 37 degrees c under static condition in effluent supplemented with 0.5% glucose, 0.1% kh(2) ...200716473005
new triazole and triazolothiadiazine derivatives as possible antimicrobial agents.triazole and triazoles fused with six-membered ring systems are found to possess diverse applications in the fields of medicine, agriculture and industry. the new 1,2,4-triazole and 1,2,4-triazolo[3,4-b][1,3,4]thiadiazine derivatives were synthesized as novel antimicrobial agents. the reaction of 1h-indol-3-acetic acid with thiocarbohydrazide gave the 4-amino-3-mercapto-5-[(1h-indol-3-yl)methyl]-4h-1,2,4-triazole. the reaction of triazole with arylaldehydes in ethanol gave the 4-arylideneamino-3 ...200817499887
antimicrobial activity of essential oils of ocimum gratissimum l. from different populations of kenya.hydro-distilled volatile oils from the leaves of ocimum gratissimum l. (lamiaceae) of 13 populations of different silvicultural zones were evaluated for antimicrobial activity against gram positive (staphylococcus aereus, bacillus spp.) and gram negative (escherichia coli, pseudomonas aeruginosa, samonella typhi, klebisiella pneumoniae, proteus mirabilis) bacteria and a pathogenic fungus, candida albicans. all the essential oils are active to the tested microbiles with different strength. the hi ...200820161936
evaluation of phytochemical and antibacterial potential of helicteres isora l. fruits against enteric bacterial pathogens.antibacterial activities of aqueous, acetone, ethanol and methanol extracts of fruits of helicteres isora (mororphali) were studied. the fruit aqueous extracts of h. isora showed prominent antibacterial activities against e. coli, staphylococcus epidermidis, salmonella typhimurium and proteus vulgaris; moderate activity against enterobacter aerogenes, staphylococcus aureus, salmonella typhi and least activity against pseudomonas aeruginosa. the aqueous extract showed maximal, the ethanol and met ...200820161950
isolation of bacteria causing secondary bacterial infection in the lesions of cutaneous leishmaniasis.cutaneous leishmaniasis (cl) is a parasitic disease characterized by single or multiple ulcerations. secondary bacterial infection is one of the complications of the disease that can increase the tissue destruction and the resulting scar.200819882011
antimicrobial activity of acanthus ilicifolius (l.).the antimicrobial activity of alcoholic, butanolic and chloroform extracts of leaves and roots of the plant acanthus ilicifolius ware studied. ampicillin and clotrimazole were used as standard antibacterial and antifungal agents respectively. the result of the study revealed that the alcoholic extract and chloroform extract of leaves exhibited strong inhibitory action against bacillus subtilis, staphylococcus aureus, candida albicans, aspergillus fumigatus and aspergillus niger and moderate inhi ...200821369453
antimicrobial activities of the bromophenols from the red alga odonthalia corymbifera and some synthetic derivatives.a series of bromophenols was obtained by isolation from red alga odonthalia corymbifera and by reactions of bis(hydroxyphenyl)methanes with bromine. new bromophenols including 3,3',5,5'-tetrabromo-2,2',4,4'-tetrahydroxydiphenylmethane (10), a regioisomer of the potent antimicrobial natural product, together with known derivatives were synthesized in high yield. all of the isolated and synthesized compounds were tested for antimicrobial activity against gram-negative, gram-positive bacteria and f ...200818053715
detection of plasmid-mediated ampc in escherichia coli, klebsiella pneumoniae and proteus mirabilis.this study investigated the prevalence of plasmid-mediated ampc production in selected clinical isolates of escherichia coli, klebsiella species and proteus mirabilis, and compared the results of boronic acid disc screening with conventional susceptibility testing for the detection of ampc-positive isolates.200818057079
presence of aquaporin and v-atpase on the contractile vacuole of amoeba proteus.the results of water permeability measurements suggest the presence of an aqp (aquaporin) in the membrane of the cv (contractile vacuole) in amoeba proteus [nishihara, shimmen and sonobe (2004) cell struct. funct. 29, 85-90].200818004980
rmtc 16s rrna methyltransferase in australia. 200818025117
salmonella genomic island 1 (sgi1), variant sgi1-i, and new variant sgi1-o in proteus mirabilis clinical and food isolates from china.salmonella genomic island 1 (sgi1) and variants (sgi1-i and the new variant sgi1-o) were mapped in five strains of proteus mirabilis isolated from humans and food in china. sequencing showed that sgi1 and variants were integrated at the 3' end of the chromosomal thdf gene as previously described for salmonella strains.200818025121
synthesis, characterization and antibacterial activity of cobalt(iii) complexes with pyridine-amide ligands.the ligands 2-(n-(x-pyridyl)carbamoyl)pyridine (x=2, 3 or 4 for hl1-hl3, respectively) and 2,6-bis(n-(y-pyridyl)carbamoyl)pyridine (y=2, 3 or 4 for h2l4-h2l6, respectively) in their mono- and di-deprotonated forms have been used to synthesize kinetically stable cobalt(iii) compounds [co(l1-3)3] (1-3) and na[co(l4-6)2] (4-6), respectively. the co(iii) ion is in octahedral environment and is surrounded by three bidentate ligands in complexes 1-3 and two tridentate ligands in complexes 4-6. ligands ...200817959275
type 2 segmental cowden disease vs. proteus syndrome: reply from authors. 200817999697
rheumatoid arthritis in smokers could be linked to proteus urinary tract infections.there is an interplay between genetic and environmental factors in the induction and development of rheumatoid arthritis (ra). the ra-associated hla-drb1 alleles which contain the shared epitope moiety as well as microbial triggers such as proteus are involved in the aetiopathogenesis of this disease. increased association between proteus urinary tract infections (utis) and ra on one hand and the link between smoking and utis on the other hand could explain the increased frequency of ra among sm ...200817983708
proteus syndrome associated with hemimegalencephaly and ohtahara syndrome: report of two cases.the authors report two cases of brazilian children with most of the common syndromic features of proteus syndrome, such as asymmetric overgrowth of tissues, skin abnormalities, hypotonia and mental retardation. in both patients, a refractory epilepsy, compatible with ohtahara syndrome, as well as hemimegalencephaly, with asymmetric distribution of facial fat, were also diagnosed.200818082431
hydrogen and polyhydroxybutyrate producing abilities of microbes from diverse habitats by dark fermentative process.thirty five bacterial isolates from diverse environmental sources such as contaminated food, nitrogen rich soil, activated sludges from pesticide and oil refineries effluent treatment plants were found to belong to bacillus, bordetella, enterobacter, proteus, and pseudomonas sp. on the basis of 16s rrna gene sequence analysis. under dark fermentative conditions, maximum hydrogen (h(2)) yields (mol/mol of glucose added) were recorded to be 0.68 with enterobacter aerogenes egu16 followed by 0.63 w ...200818083024
experimental supratrigonal cystectomy: ii--evaluation of urinary calculi, infection, and bladder dysfunction in the pathogenesis of renal failure.the objective of this study was to evaluate the role of urolithiasis, infection, and bladder dysfunction in the pathogenesis of renal failure in rats subjected to supratrigonal cystectomy. one group of sprague-dawley rats was submitted to supratrigonal cystectomy, a second to cystectomy during which a suspension of proteus mirabilis was injected into the bladder stump, and a third to sham surgery (controls). the animals were sacrificed two months after surgery. blood pressure and serum urea and ...200818085427
isolation and purification of enterocin e-760 with broad antimicrobial activity against gram-positive and gram-negative bacteria.strain nrrl b-30745, isolated from chicken ceca and identified as enterococcus durans, enterococcus faecium, or enterococcus hirae, was initially identified as antagonistic to campylobacter jejuni. the isolate produced a 5,362-da bacteriocin (enterocin) that inhibits the growth of salmonella enterica serovar enteritidis, s. enterica serovar choleraesuis, s. enterica serovar typhimurium, s. enterica serovar gallinarum, escherichia coli o157:h7, yersinia enterocolitica, citrobacter freundii, klebs ...200818086839
activities of the frog skin peptide, ascaphin-8 and its lysine-substituted analogs against clinical isolates of extended-spectrum beta-lactamase (esbl) producing bacteria.extended-spectrum beta-lactamase (esbl)-producing gram-negative bacteria are becoming increasingly prevalent and their antibiotic resistance necessitates novel therapeutic intervention. ascaphin-8 is a cationic alpha-helical peptide that shows broad-spectrum antibacterial activity but is also toxic to human erythrocytes (lc(50)= 55 microm). this study assesses the activity of ascaphin-8, and a series of l-lysine-substituted analogs, against a range of clinical isolates of esbl-producing bacteria ...200818068868
in vitro activities of tigecycline combined with other antimicrobials against multiresistant gram-positive and gram-negative pathogens.to test the activity of tigecycline combined with 16 antimicrobials in vitro against 22 gram-positive and 55 gram-negative clinical isolates.200818033780
antibacterial activity of plants used in traditional medicines of ghana with particular reference to mrsa.: in an ethno botanical survey carried out in the akwapim-north district of the republic of ghana, 25 plant species, used in traditional medicine to treat skin disease and/or to treat antimicrobial (viral, bacterial or protozoan) infections were identified.200818096337
in vitro activities of doripenem and six comparator drugs against 423 aerobic and anaerobic bacterial isolates from infected diabetic foot wounds.against 182 anaerobe and 241 aerobe strains obtained from diabetic foot infections, doripenem was the most active carbapenem against pseudomonas aeruginosa (mic(90), 2 microg/ml), more active than imipenem against proteus mirabilis, and ertapenem was more active against escherichia coli and klebsiella spp. the mic(50) and mic(90) values were < or =0.125 microg/ml for methicillin-sensitive staphylococcus aureus and all streptococci and 0.25/1 for bacteroides fragilis.200818070958
antibiotic policy and prescribing strategies for therapy of extended-spectrum beta-lactamase-producing enterobacteriaceae: the role of piperacillin-tazobactam.therapy of infections caused by extended-spectrum beta-lactamase (esbl)-producing bacteria with an antimicrobial to which they are resistant results in treatment failure, higher cost and increased mortality. the clsi recommends reporting esbl-producing strains of escherichia coli, klebsiella spp. and proteus spp. as resistant to all penicillin, true cephalosporin and monobactam antimicrobials, but as susceptible to beta-lactam-beta-lactamase inhibitor combinations, including piperacillin-tazobac ...200818154544
production and characterization of a bioflocculant by proteus mirabilis tj-1.a bioflocculant tj-f1 with high flocculating activity, produced by strain tj-1 from a mixed activated sludge, was investigated with regard to its production and characterization. by 16s rdna sequence and biochemical and physiological characteristics, strain tj-1 was identified as proteus mirabilis. the most preferred carbon source, nitrogen source and c/n ratio (w/w) for strain tj-1 to produce the bioflocculant were found to be glucose, peptone and 10, respectively. tj-f1 production could be gre ...200818155905
structure and mode of action of the antimicrobial peptide arenicin.the solution structure and the mode of action of arenicin isoform 1, an antimicrobial peptide with a unique 18-residue loop structure, from the lugworm arenicola marina were elucidated here. arenicin folds into a two-stranded antiparallel beta-sheet. it exhibits high antibacterial activity at 37 and 4 degrees c against gram-negative bacteria, including polymyxin b-resistant proteus mirabilis. bacterial killing occurs within minutes and is accompanied by membrane permeabilization, membrane detach ...200817935487
prevalence of extended-spectrum beta-lactamases in proteus mirabilis in a taiwanese university hospital, 1999 to 2005: identification of a novel ctx-m enzyme (ctx-m-66).a total of 1574 nonduplicate proteus mirabilis isolates collected at a taiwanese hospital during 1999 to 2005 were analyzed for production of extended-spectrum beta-lactamases (esbls). forty-four esbl-producing isolates including 22 ctx-m-14, 18 ctx-m-3, 2 ctx-m-24, and 2 ctx-m-66 producers were detected, and the proportion of esbl producers increased from 0.7% in 1999 to approximately 6% after 2002. ctx-m-66 is a novel variant of ctx-m esbls that differs from ctx-m-3 by a ser to asn change at a ...200817913434
microbial keratitis in a case of lamellar ichthyosis.we report a case of microbial keratitis in a patient with lamellar ichthyosis. an 11-year-old boy, a known case of lamellar ichthyosis, presented with microbial keratitis. microbiological evaluation of corneal scraping revealed a mixed infection caused by gram-negative bacilli and gram-positive cocci. he was treated with topical ciprofloxacin and fortified cefazolin eyedrops. microbial keratitis in patients with lamellar ichthyosis has a poor prognosis.200817828596
endoscopic treatment of pituitary abscess: two case reports and literature review.pituitary abscess is a rare entity and the correct diagnosis is still difficult before surgery. more than 210 cases have been reported in the medical literature, mostly in the form of isolated case reports. we report two cases of pituitary abscess treated endoscopically and we review the literature. a 30-year-old woman and a 35-year-old man were admitted with a history of pituitary dysfunction. patient 1 presented with polyuria, polydipsia, amenorrhea, headache, and visual impairment. magnetic r ...200817899232
diverse tetracycline resistant bacteria and resistance genes from coastal waters of jiaozhou bay.environmental microbiology investigation was carried out in jiaozhou bay to determine the source and distribution of tetracycline-resistant bacteria and their resistance mechanisms. at least 25 species or the equivalent molecular phylogenetic taxa in 16 genera of resistant bacteria could be identified based on 16s ribosomal deoxyribonucleic acid sequence analysis. enterobacteriaceae, pseudomonadaceae, and vibrionaceae constituted the majority of the typical resistant isolates. indigenous estuari ...200817668262
urease-positive bacteria in the stomach induce a false-positive reaction in a urea breath test for diagnosis of helicobacter pylori infection.this study investigated the influence of urease-positive non-helicobacter pylori bacteria on the results of a urea breath test (ubt) to evaluate the diagnostic utility of a ubt using film-coated [(13)c]urea tablets. the ubt was performed in 102 patients treated with a proton pump inhibitor and antibiotics for the eradication of h. pylori. urease-producing bacteria other than h. pylori were isolated and identified from the oral cavity and stomach. in 4/102 patients, the ubt gave false-positive re ...200818566138
biofilm structure differentiation based on multi-resolution analysis.quantitative parameters for describing the morphology of biofilms are crucial towards establishing the influence of growing conditions on biofilm structure. parameters used in earlier studies generally fail to differentiate complex three-dimensional structures. this article presents a novel approach of defining a parameter vector based on the energy signature of multi-resolution analysis, which was applied to differentiating biofilm structures from confocal laser scanning microscopy (clsm) biofi ...200818568669
antibacterial and preliminary phytochemical and physico-chemical analysis of eucalyptus citriodora hk leaf.the present communication deals with some studies on the antibacterial, physico-chemical and phytochemical parameters of different extracts of eucalyptus citriodora leaf. the antibacterial study was performed using the agar ditch method on some clinically important bacteria, namely pseudomonas pseudoalcaligenes, proteus vulgaris, citrobacter freundii, staphylococcus subflava, bacillus megaterium, and enterobacter aerogenes. physico-chemical parameters namely water, methanol, 1,4-dioxane, dmf, ac ...200818569717
tapering immunosuppressive therapy significantly improves in vivo cutaneous delayed type hypersensitivity responses.immunosuppressive therapy affects cell-mediated immunity and thereby increases the frequency of infections and malignancies in transplanted patients. we questioned whether reducing the immunosuppressive dose in stable kidney transplant patients has an in vivo effect on cutaneous delayed type hypersensitivity responses (dth) reflecting cell-mediated immunity. we measured dth responses to recall antigens (tetanus, diphteria, streptococcus, tuberculin, candida, trychophyton, proteus, glycerin contr ...200818573662
flavonols and an oxychromonol from piliostigma reticulatum.the leaf extract from the plant piliostigma reticulatum was found to exhibit antimicrobial activity against some bacteria and fungi such as staphylococcus aureus (nctc 6571), escherichia coli (nctc 10418), bacillus subtilis (nctc 8236), proteus vulgaris (nctc 4175), aspergillus niger (atcc 10578) and candida albicans (atcc 10231). upon investigation of the chemical constituents present in the leaf extract, a total of seven compounds were isolated and their structures were unambiguously establish ...200818582911
[evaluation of the phoenix automated microbiology system for detecting extended-spectrum beta-lactamase in escherichia coli, klebsiella species and proteus mirabilis].the aim of this study was to compare the bd phoenix (beckton dickinson diagnostic systems, usa) extended-spectrum beta-lactamase (esbl) test with the clinical and laboratory standards institute (clsi) esbl phenotypic confirmatory test by disk diffusion (clsi esbl test) in escherichia coli, klebsiella pneumoniae, klebsiella oxytoca and proteus mirabilis.200818594169
[etiology of acute and chronic pyelonephritis in children in khabarovsk region].microflora of urinary tract was studied in 419 children aged 1 - 17 years and hospitalized due to acute or chronic pyelonephritis. etiology of inflammatory process was established in 57.8% of cases. according to our study, etiologic structure of causative agents of pyelonephritis did not differ from all-russian data. the leading positions belonged to gram-negative microorganisms from enterobacteriaceae family: escherichia coli, proteus mirabilis, and klebsiella spp. results of the study point to ...200818595469
the mtor pathway and its role in human genetic diseases.the signalling components upstream and downstream of the protein kinase mammalian target of rapamycin (mtor) are frequently altered in a wide variety of human diseases. upstream of mtor key signalling molecules are the small gtpase ras, the lipid kinase pi3k, the akt kinase, and the gtpase rheb, which are known to be deregulated in many human cancers. mutations in the mtor pathway component genes tsc1, tsc2, lkb1, pten, vhl, nf1 and pkd1 trigger the development of the syndromes tuberous sclerosi ...200818598780
[influence of the mixed inoculum on the species-identification and antimicrobial susceptibility test results by the automated microbial systems].the influence of the mixed inoculum on the species-identification and antimicrobial susceptibility test results was evaluated for the three automated microbial systems, walkaway-40 (dade microscan, west sacramento, ca, usa), vitek 2 compact (biomérieux, marcy l'etoile, france) and raisus (nissui pharmaceutical, tokyo). in the evaluation, two different species or two different strains were mixed in serial ratios and adjusted to the inoculum cell suspension for the respective systems, and then tes ...200818516962
[in-vitro activity of panipenem against clinical isolates in 2006].the antimicrobial activity of various antibiotics against clinical bacterial isolates recovered from patients with infectious diseases at the medical facilities in the kanto region between march and september 2006 was evaluated. a total of 1030 clinical isolates were available for susceptibility tests: 420 aerobic gram-positive organisms, 520 aerobic gram-negative organisms, 30 anaerobic gram-positive organisms and 60 anaerobic gram-negative pathogens. antimicrobial susceptibility data for strep ...200818536215
detection of a lysozyme inhibitor in proteus mirabilis by a new reverse zymogram method.a reverse zymogram method for the detection of bacterial lysozyme inhibitors was developed. this method was validated by using a periplasmic protein extract of escherichia coli containing a known inhibitor and subsequently led to the detection of a new proteinaceous hen egg white lysozyme inhibitor in proteus mirabilis.200818539812
contemporary management of uncomplicated urinary tract infections.uncomplicated urinary tract infections (uutis) are common in adult women across the entire age spectrum, with mean annual incidences of approximately 15% and 10% in those aged 15-39 and 40-79 years, respectively. by definition, utis in males or pregnant females and those associated with risk factors known to increase the risk of infection or treatment failure (e.g. acquisition in a hospital setting, presence of an indwelling urinary catheter, urinary tract instrumentation/interventions, diabetes ...200818547131
observations on the development of the crystalline bacterial biofilms that encrust and block foley catheters.the care of many patients undergoing long-term bladder catheterisation is complicated when the flow of urine through the catheter is blocked by encrustation. the problem results from infection by urease-producing bacteria, especially proteus mirabilis, and the subsequent formation of crystalline biofilms on the catheter. the aim of this study was to discover how p. mirabilis initiates the development of these crystalline biofilms. the early stages in the formation of the biofilms were observed o ...200818550219
afebrile emphysematous pyelonephritis. 200818550410
characterization of antimicrobial resistance and class 1 integrons in enterobacteriaceae isolated from mediterranean herring gulls (larus cachinnans).mediterranean herring gulls (larus cachinnans) were investigated as a possible reservoir of antibiotic resistant bacteria and of cassette-borne resistance genes located in class 1 integrons. two hundred and fourteen isolates of the family enterobacteriaceae were collected from cloacal swabs of 92 chicks captured in a natural reserve in the north east of italy. they showed high percentages of resistance to ampicillin and streptomycin. high percentages of resistance to trimethoprim/sulfamethoxazol ...200818476779
complications of chronic otitis media with cholesteatoma during a 10-year period in kosovo.we review and discuss the results of treatments for complications of cholesteatomatous chronic otitis media (ccom) in a tertiary health care center. in a retrospective study, the medical records of patients with complications of ccom who had undergone surgical treatment at the ent clinic of the university clinical center of kosovo for the period 1994-2004 were reviewed. from a total of 1,803 patients suffering from ccom, in 91 patients, 55 (60.4%) men and 36 (39.6) women, one or two complication ...200818478242
incisional surgical infection in heart transplantation.incisional surgical site infections (issis) are common bacterial infections in heart transplantation (ht). the purpose of this study was to determine the incidence, etiology, timing, and risk factors for issis.200818482203
ulcer infection by esbetal-producing proteus mirabilis: a case report.in this article, a case of decubitus ulcer infection caused by an extended-spectrum beta-lactamase-producing proteus mirabilis strain, successfully treated with oral amoxicillin-clavulanate (1-month therapy) is described. this article focuses on diffusion and clinical effect of extended-spectrum beta-lactamases-producing proteus mirabilis on treatment of gram negative lower extremity infections.200818483010
proteus2: a web server for comprehensive protein structure prediction and structure-based annotation.proteus2 is a web server designed to support comprehensive protein structure prediction and structure-based annotation. proteus2 accepts either single sequences (for directed studies) or multiple sequences (for whole proteome annotation) and predicts the secondary and, if possible, tertiary structure of the query protein(s). unlike most other tools or servers, proteus2 bundles signal peptide identification, transmembrane helix prediction, transmembrane beta-strand prediction, secondary structure ...200818483082
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