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an in vitro assessment of the antibacterial effect of garlic (allium sativum) on bacterial isolates from wound infections.garlic (allium sativum) has come to be seen as an all rounded treatment for preventing wound infection, common cold, malaria, cough and lung tuberculosis, hypertension, sexually transmitted diseases, mental illness, kidney diseases, liver diseases, asthma, diabetes.200617370439
[evaluation of vitek 2 performance for identifying extended spectrum beta-lactamases in enterobacteriaceae "other than escherichia coli, proteus mirabilis and klebsiella spp"].production of beta-lactamases is the main resistance mechanism of gram-negative bacteria against beta-lactam antibiotics. extended spectrum beta-lactamases (esbls) have the ability to hydrolyze a broader spectrum of beta-lactam drugs. hence rapid, accurate detection of this resistance mechanism is extremely important to guide proper patient antimicrobial therapy. these enzymes are most commonly produced by klebsiella spp. and escherichia coli, but may also occur widely in other gram-negative bac ...200617380089
perinephric abscess secondary to a staghorn calculus presenting as a subcutaneous abscess.perinephric abscesses often present with flank pain and signs of sepsis, and are known to discharge to the skin. we report a case of a young patient presenting with a 48-hour history of painful swelling in her right renal angle following a urinary tract infection and no overt signs or symptoms of sepsis. plain abdominal films showed a perinephric abscess secondary to a staghorn calculus in the upper pole of the right kidney. on incision and drainage, 700 ml of pus was drained freely. the patient ...200617324312
spontaneous bacterial peritonitis causing serratia marcescens and proteus mirabilis ventriculoperitoneal shunt infection. case report.the authors report their experience treating a polymicrobial ventriculoperitoneal (vp) shunt infection in a developmentally delayed 21-year-old woman. cerebrospinal fluid (csf) cultures grew serratia marcescens and proteus mirabilis. on admission and throughout her hospitalization, results of physical examination of her abdomen were normal, and radiographic studies showed no evidence of bowel perforation or pseudocyst formation. contrast-enhanced computed tomography of the abdomen revealed a sma ...200617219841
increased resistance to first-line agents among bacterial pathogens isolated from urinary tract infections in latin america: time for local guidelines?emerging resistance phenotypes and antimicrobial resistance rates among pathogens recovered from community-acquired urinary tract infections (ca-uti) is an increasing problem in specific regions, limiting therapeutic options. as part of the sentry antimicrobial surveillance program, a total of 611 isolates were collected in 2003 from patients with ca-uti presenting at latin american medical centers. each strain was tested in a central laboratory using clinical laboratory standard institute (clsi ...200617160281
bacterial isolates of blood cultures in patients with suspected septicaemia in ilorin, nigeria.periodic bacteriologic surveillance of blood cultures is a necessity. to determine currently prevalent bacterial pathogens of blood cultures in patients with suspected septicaemia in ilorin. nigeria. three thousand and fifty-seven patients from all age and sex groups with clinical suspicion of septicaemia underwent bacteriologic screening of their blood specimen over a 13 month period starting from january. 2003. twenty point three percent were positive for bacterial growth. an incidence of 41.7 ...200617209308
skull base osteomyelitis presenting as villaret's syndrome.this report documents a 47-year-old male who developed acute deficits of the ix, x, xi, xii cranial nerves and horner's symptoms, consistent with villaret's syndrome. neuroimaging studies demonstrated an osteolytic lesion in the skull base involving the clivus and jugular foramen. the patient recovered after the antibiotic treatment for proteus mirabilis infection. we suggest that villaret's syndrome can be a rare presentation of skull base osteomyelitis.200617214089
more than just a bump: the hamartoma syndromes.the hamartoma syndromes detailed in this review are just a few examples of the many genodermatoses now known to be associated with uncontrolled tumor proliferation secondary to mutations in tumor suppressor genes. knowledge gained through the study of these syndromes has not only improved our understanding of patients afflicted with such conditions, but has also led to significant insight into the important role tumor suppressor genes play in preventing tumor formation and in carcinogenesis. as ...200617249301
[bacteriological evaluation in chronic otitis media].in chronic otitis media bacterial infection is often a cause of exacerbation in the clinical course and failure in treatment. the aim of the study was to investigate the incidence and profile of the aerobic bacterial flora in chronic otitis media and to determine the drug susceptibility of selected isolated bacteria.200617263251
in vivo evaluation of teicoplanin- and calcium sulfate-loaded pmma bone cement in preventing implant-related osteomyelitis in rats.the objective of this study was to evaluate the efficacy of teicoplanin- and calcium sulphate-loaded polymethylmethacrylate (pmma) bone cements in preventing experimental implant-related osteomyelitis in rats. four groups of antibiotic-loaded rods were prepared and were implanted into the lateral condylus of the rat femur after inoculation of staphylococcus aureus. the effectiveness of these were assessed microbiologically, radiographically, and histopathologically. radiographic evaluation revea ...200617267341
classification of proteus mirabilis tg 115 and ccug 10701 into the proteus o23 serogroup based on chemical and serological studies of o-polysaccharides.bacteria of the genus proteus are a common cause of urinary tract infections. the o-polysaccharide (ops) chain of their lipopolysaccharide (lps) defines the serological specificity of strains. based on the ops structures and the immunospecificity of the lps, proteus strains have been classified into 74 o-serogroups.200617122881
in-vitro activity of triclosan-eluting ureteral stents against common bacterial uropathogens.ureteral stents are commonly used in urology today, but the biofilms that form on them within hours of placement may harbor bacteria that can result in infection or encrustation. triclosan is an antimicrobial commonly used in consumer and medical products that inhibits bacterial fatty-acid synthesis. the bactericidal and bacteriostatic effect of a triclosan-eluting ureteral stent was tested against clinical isolates of common bacterial uropathogens in an in-vitro setting.200617144870
[comparison of different methods in order to identify proteus spp].comparison of different methods in order to identify proteus spp. the objectives were: (a) to identify proteus strains to species level, following farmer's and o'hara's conventional biochemical reactions; b) to evaluate the sensitivity and specificity of both the api 20e method and a schema of reduced reactions (tsi and mio agar: motility, indole and ornithine) comparing them with conventional methodology, and c) to evaluate the utility of sds-page (total proteins) in order to identify proteus s ...200617152651
capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.the nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. this dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. the values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pi markers and were found comparable with pi of the native compounds. the m ...200617165837
comparison of pharmacodynamic and pharmacokinetic indices of efficacy for 5 fluoroquinolones toward pathogens of dogs and cats.fluoroquinolones are often used interchangeably in dogs and cats.200617186841
bacteriology of chronic discharging ears in port harcourt, nigeria.the objective of the study was to isolate and identify aerobic bacteria in chronic discharging ears and determine their antibiotic susceptibilities, which is essential for reasonable empiric treatment.200617191422
[using antibiotics in urinary tract infection in a first level of attention health care unit in bogotá, colombia].describing using antibiotics for urinary tract infection in a first level of attention health care unit.200617191601
pathogenic colonization of the stomach in enterally fed elderly patients: comparing percutaneous endoscopic gastrostomy with nasogastric tube.to compare the gastric juice microbiota of older people fed through a percutaneous endoscopic gastrostomy tube (peg) with that of those fed through a nasogastric tube (ngt).200617198497
accelerated detection of extended-spectrum beta-lactamases in clinical isolates of enterobacteriaceae.a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum beta-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esbl-producers by the standard clinical laboratory stand ...200617201201
comparison of three techniques for the diagnosis of urinary tract infections in dogs with urolithiasis.to identify an appropriate sampling technique(s) to accurately detect the bacteria causing urinary tract infections in dogs with urolithiasis.200617201824
[pseudomonas aeruginosa colonisation in bronchiectatic patients and clinical reflections].bronchiectasis is characterized with irreversible dilatation according to destruction of epithelium, elastic and muscular layer. most important cause of bronchiectasis is chronic bacterial infections. pseudomonas aeruginosa colonisation is frequently seen in bronchiectatic patients. we aimed to find out p. aeruginosa colonisation frequency and clinical, radiological and spirometric reflections due to colonisation. we analysed 83 cases retrospectively. mean age was 58.2 and 54.2% of them were fem ...200617203422
[short communication: prevalence of extended spectrum beta-lactamases in gram negative rods: data of 2001-2004 period].this study was undertaken to determine the prevalence of extended spectrum beta-lactamase (esbl) production by gram negative rods (gnrs) isolated from various clinical specimens (3.240 urine, 336 pus, 277 feces, 83 blood, 38 sputum) sent to our hospital laboratory, between 2001-2004. of isolated bacteria 71.4% were identified as escherichia coli, 11.1% were klebsiella spp, 4.2% were salmonella spp, 3.7% were pseudomonas spp, 3.5% were proteus spp, 2.9% were shigella spp, 2.3% were enterobacter s ...200617205693
plasmid determined resistance to quinolones in clinical isolates of gram-negative bacilli.a total of 166 strains of gram-negative bacilli comprising 65 klebsiella species, 22 escherichia coli, 39 pseudomonas aeruginosa, 20 proteus species, 18 other pseudomonas species and 2 salmonella typhi, were isolated from clinical specimens in the university college hospital, ibadan. antimicrobial susceptibility testing and plasmid profiling of the strains were done. ceftazidine had the highest antibacterial activity of 83.1% compared to the fluoroquinolones followed by ciprofloxacin (78.3%), pe ...200617722809
antifungal and antibacterial activities of mexican tarragon (tagetes lucida).mexican tarragon (tagetes lucida cv. asteraceae: campanulatae) is an important, nutritious plant and an effective herbal medicine. seven coumarins, 7,8-dihydroxycoumarin (4), umbelliferone (7-hydroxycoumarin) (5), scoparone (6,7-dimethoxycoumarin) (7), esculetin (6,7-dihydroxycoumarin) (11), 6-hydroxy-7-methoxycoumarin (12), herniarin (7-methoxycoumarin) (13), and scopoletin (6-methoxy-7-hydroxycoumarin) (14), and three flavonoids, patuletin (18), quercetin (19), and quercetagetin (20), were iso ...200619127719
the establishment of resistance phenotypes for bacteria isolated from outpatients in urine cultures.from 1911 outpatients, who addressed a timi┼čoara private clinical laboratory, from january to december 2005, we collected 1,889 urine cultures, 431 being positive. bacteria identification was generally done using morphological, cultural, biochemical characters and pathogenicity tests. sensitivity testing to antimicrobial medical drugs was done by using the classical diffusion kirby-bauer method and the automatic analyzer osiris, also. the main bacteria involved in the etiology of these infection ...200618389723
microbial degradation of palm (elaeis guineensis) biodiesel.the kinetics of biodegradation of palm-derived fatty methyl and ethyl esters (elaeis guineensis biodiesel) by a wild-type aerobic bacterial population was measured at 20 degrees c, as the rate of oxygen uptake by a manometric technique. the methyl and ethyl biodiesels were obtained by potassium-hydroxide catalysed trans-esterification of palm oil, respectively. the bacterial flora included the genera bacillus, proteus, pseudomonas, citrobacter and enterobacter. the rate of oxygen uptake for palm ...200618457175
common pathogens isolated in diabetic foot infection in bir hospital.foot ulcers are a frequent complication of patients suffering with diabetes mellitus, accounting for up to 20% of diabetes-related hospital admission . secondary infection of these ulcers is by far the leading cause of amputation of feet and legs and the polymicrobial nature of diabetic foot infection has been well documented in the literature. the present study sought to reveal the bacterial etiology of diabetic foot ulcer in patients presenting to bir hospital.200618603922
composition and antimicrobial activity of essential oil from the fruits of amomum cannicarpum.essential oil from the fruits of amomum cannicarpum (wight) bentham ex baker (zingiberaceae) was hydrodistilled and characterized by gas chromatography-mass spectrometry (gc-ms). major constituents of the oil were beta-pinene (14.00%), elemol (10.45%) and alpha-cadinol (8.50%). thirty-three (91.48%) out of forty-one constituents were identified by gc-ms and subsequent data analysis. antimicrobial activity of the oil against gram-positive and gram-negative bacteria and some fungi, was determined ...200619839139
production and characterisation of monoclonal antibodies specific for escherichia coli o157:h7.seven monoclonal antibodies (mabs) specific for escherichia coli o157:h7, one of the major causes of haemorrhagic colitis in humans, were produced by immunising balb/c mice with the strain e. coli o157:h7. these monoclonal antibodies do not cross-react with other bacteria such as salmonella enterica serovar typhimurium, e. coli o14, e. coli jm109, s. enterica serovar enteritidis, s. panama, s. saintpaul, s. derby, s. muenchen, s. bredeney, s. hadar, yersinia enterocolitica, proteus vulgaris, shi ...200620429059
evaluation of antimicrobial activity of the stem bark of cylicodiscus gabunensis (mimosaceae).ethyl acetate (ea) extract of the stem bark of cylicodiscus gabunensis (cg) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: escherichia coli, klebsiella pneumoniae, shigella dysenteriae, shigella flexneri, morganella morganii, proteus vulgaris, proteus mirabilis, salmonella typhi, citrobacter freundii, enterobacter cloacae, enterobacter agglomerans, staphylococcus aureus, streptococcus feacalis, pseudomonas aeruginosa ...200620162076
intra-pericardial rupture of bacterial hepatic abscess: an unusual cause of 'cardiac' chest pain.we report a rare case of bacterial pericarditis secondary to rupture of liver abscess into the pericardium. the patient presented with cardiac-sounding chest pain and with normal ecg, cxr and troponin-i assay. the initial echocardiogram showed minimal pericardial fluid but, when he later developed overt clinical signs of cardiac tamponade, a ct scan of chest and abdomen revealed a pronounced pericardial effusion. it also revealed an abscess located in the left lobe of liver and this had apparent ...200621611623
the therapeutic alliance--a proteus in disguise.the therapeutic alliance is a vital component of not only the psychoanalytic relation but of all therapeutic encounters between psychotherapist and patient. despite the universal application and realization of the alliance concept in therapeutic endeavors, it is often ignored as an operative concept in the therapeutic theoretical armamentarium or is formulated in alternative terms. it also comes into play implicitly, even when the concept is formally dismissed as irrelevant. this discussion addr ...200622122095
confirmation of presumptive salmonella colonies contaminated with proteus swarming using the polymerase chain reaction (pcr) method.in mexico, zero tolerance regulation is practiced regarding salmonella in food products. the presence of which is verified by the procedure described in nom 114-ssa-1994. during the period between august 2002 and march 2003, 245 food samples were tested using this procedure in the central laboratories of the department of health for the state of jalisco (ceeslab). of these 245 samples, 35 showed presumptive colonies contaminated with proteus swarm cells even after selective isolation. these swar ...200718693548
bacteriology of wound infections in the surgical wards of a teaching hospital in enugu, nigeria.during a 24-month period, january 2001 - december 2002, 404 wound swabs from 390 patients made up of 280 from surgical wounds, 92 from ulcers and 32 from other wounds like lacerations and discharging sinuses were studied. the prevalence of bacterial organisms and their antibiotic susceptibility pattern was analyzed, using standard bacteriologic techniques. two hundred and sixty four organisms (69.8%) were isolated from surgical wounds, 82 (21.7%) from ulcers and 32 (8.5%) from other wounds. coli ...200718564650
tigecycline in the treatment of complicated intra-abdominal and complicated skin and skin structure infections.tigecycline, a glycylcycline related to the tetracycline class of antibiotics, represents a new option for the treatment of complicated intra-abdominal and complicated skin and skin structure infections. it displays favorable activity in vitro against the most common causative gram-positive, gram-negative and anaerobic pathogens. in addition, tigecycline demonstrates activity against drug-resistant pathogens such as methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, a ...200718516315
epidermal nevus syndromes.the term "epidermal nevus syndrome" (ens) has been used to describe the association of epidermal hamartomas and extra-cutaneous abnormalities. epidermal nevi follow the lines of blaschko. the majority of the extra-cutaneous manifestations involve the brain, eye, and skeletal systems. several subsets with characteristic features have been delineated including the nevus sebaceous syndrome, proteus syndrome, child syndrome, becker nevus syndrome, nevus comedonicus syndrome, and phakomatosis pigment ...200718395670
[new perspectives of the phage therapy].a solution to the problem of the increasing number of antibiotic-resistant bacterial strains can be the use of rational phage therapy. in the past, bacteriophages (phages) were often used for the treatment and prevention of infections and unlike antibiotic therapy, phage therapy caused almost no serious side effects. while previously several preparations containing whole phage particles were available for phage therapy, currently, the isolation of well characterised and purified phage components ...200718320502
antimicrobial resistance patterns of clinical bacterial isolates in southwestern ethiopia.the aim of this study was to assess the pattern of bacterial resistance to antimicrobial agents in a hospital setting in southwest ethiopia.200718326346
serological and structural characterization of the o-antigens of the unclassified proteus mirabilis strains tg 83, tg 319, and ccug 10700 (oa).lipopolysaccharide (endotoxin, lps) is an important potential virulence factor of proteus rods. the serological specificity of the bacteria is defined by the structure of the o-polysaccharide chain (o-antigen) of the lps. until now, 76 o-serogroups have been differentiated among proteus strains.200718219766
[synthesis and antimicrobial activity of some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoyl)-d,l-methionine].in order to obtain new compounds with potential antimicrobial activity some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoil)-d,l-methionine have been synthesized. the compounds have been obtained by decyclization reaction of 2-(m-nitrophenyl)-4-(beta-p-methylthioethyl)- delta2-5-oxazolinone with various sulfonamides. the chemical structure of the new sulfonamides was confirmed by elemental analysis data and ir and 1nmr spectral measurements. the compounds were also investigated f ...200718293716
preliminary investigation on the phytochemistry and antimicrobial activity of senna alata l. flower.preliminary studies on the phytochemistry and extracts of water, methanol, chloroform and petroleum ether, of senna alata flowers were examined for antimicrobial properties. extracts tested at a final concentration of 500 microg ml(-1) produced in vitro antimicrobial activities in assays against clinical isolates of staphylococus aureus, candida albicans, escherichia coli, proteus vulgaris, pseudomonans aureginosa and bacillus subtilis. the zones of inhibitions produced by the extracts in agar d ...200719069869
studies on antimicrobial activity of perionyx excavatus.the antimicrobial activity of vermiextract was assessed against four human pathogenic microorganisms. especially strains of proteus species are a prominent cause of urinary tract infection in children. the present investigation deals with particular urinary tract pathogenic organisms like e. coli, proteus, providencia, morganella species. screening was performed by disc diffusion method. the zone of inhibition range from 17 to 19 mm against 1.0x10(8) cells of each culture at 1 mg concentration o ...200719090177
antibacterial activity of some plant extracts used in folk medicine.in the present work, selected plants were screened for their potential antibacterial activity. for evaluating antibacterial activity, both aqueous and organic solvent methanol was used. the plants screened were ocimum sanctum, jatropha gossypifolia, boerhavia diffusa, azadirachta indica, solidago virgaurea, and commelina benghalensis. the antibacterial activity was assessed against six bacterial strains--pseudomonas testosteroni, staphylococcus epidermidis, klebsiella pneumoniae, bacillus subtil ...200718928141
occurrence of proteus mirabilis associated with two species of venezuelan oysters.the fecal contamination of raw seafood by indicators and opportunistic pathogenic microorganisms represents a public health concern. the objective of this study was to investigate the presence of enteric bacteria colonizing oysters collected from a venezuelan touristic area. oyster samples were collected at the northwestern coast of venezuela and local salinity, ph, temperature, and dissolved oxygen of seawater were recorded. total and fecal coliforms were measured for the assessment of the micr ...200718157401
resin-salve from norway spruce--a potential method to treat infected chronic skin ulcers?the home-made resin salve from norway spruce is traditionally and widely used in folk medicine to heal various skin infections and wounds in northern finland. we have performed laboratory studies to solve the mechanism of resin salve. the resin salve exhibited a bacteriostatic effect against all tested gram-positive bacteria important in human medicine including methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus (vre), but was not effective against gram-nega ...200719356034
first report of the emergence of ctx-m-type extended-spectrum beta-lactamases (esbls) as the predominant esbl isolated in a u.s. health care system.ctx-m-type extended-spectrum beta-lactamases (esbls) have become increasingly common worldwide, with the notable exception of the united states, where tem- and shv-type esbls have appeared to predominate. we have noted the emergence of esbls in our health care system (the university health system in san antonio, tx), especially in escherichia coli isolates, that preferentially hydrolyze cefotaxime rather than ceftazidime, suggesting the possibility of ctx-m-type enzymes. microbiology laboratory ...200717724160
prevalence of hospital-acquired infection in a moroccan university hospital.infection control and hospital-acquired infection (hai) prevalence reports from developing countries are often not well established because of the lack of staff and resources. the aim of this study was to estimate the prevalence of hai in a moroccan hospital as well as to identify its predisposing factors.200717660013
a case of proteus syndrome with lateral embryonal vein and frontal intraosseous lipoma.a patient with proteus syndrome is reported with heretofore unreported features including a frontal intraosseous lipoma and a lateral embryonal leg vein (servelle) associated with a venous malformation. these associated anomalies further broaden the phenotypic variability of proteus syndrome.200717760489
is it a proteus syndrome? 200717762684
acquired ampc type beta-lactamases: an emerging problem in italian long-term care and rehabilitation facilities.we report the multiple detection of proteus mirabilis isolates, from 4 different long-term care and rehabilitation facilities (ltcrfs) of northern italy, resistant to expanded-spectrum cephalosporins and cephamycins and producing an acquired ampc-like beta-lactamase, named cmy-16. genotyping by pfge showed that isolates were clonally related to each other, although not identical. in all isolates the bla(cmy16) gene was not transferable by conjugation and was found to be carried on the chromosome ...200717802913
molecular epidemiology of esbetal producing p. mirabilis strains from a long-term care and rehabilitation facility in italy.we report the detection of multidrug resistant esbetal producing proteus mirabilis isolates from a long-term care and rehabilitation facility (ltcrf) in northern italy. 53% of the collected p. mirabilis strains were esbetal producers. pcr and sequencing techniques confirmed the presence of the bla(tem-92) and bla(cmy-16) resistance genes in 23/26 (88.5%) and 3/26 (11.5%) of the esbetal producers respectively. pfge showed that the tem-92 beta-lactamase producing isolates were not clonally related ...200717802927
polymerase chain reaction for the rapid diagnosis of tuberculous meningitis.polymerase chain reaction (pcr) based on the amplification of a 169 bp dna fragment specific for the mycobacterium tuberculosis complex was evaluated for the rapid diagnosis of tuberculous meningitis (tbm). a total of 105 csf specimens from clinically suspected cases of tbm were studied. clinical details of the cases and cytochemical parameters of the csf specimens were recorded. for pcr 10 csf specimens from cases other than tbm, 4 non-mycobacterial culture isolates (one strain of e coli, one s ...200717802972
hormonal regulation of the contractile vacuole in amoeba proteus: the role of cyclic nucleotide phosphodiesterases. 200717668613
an urgent problem of aerobic gram-negative pathogen infection in complicated parapneumonic effusions or empyemas.complicated parapneumonic effusion or empyema is a troublesome disease with a high mortality. the most common involved microorganisms seem to have changed over recent decades, influenced by the introduction of new antibiotics, and the increase of immunocompromised hosts, and the elderly population. more epidemiological studies on the current bacteriology are needed to help us to empirically select adequate antibiotics.200717675765
profile of community- and hospital-acquired pneumonia cases admitted to basra general hospital, iraq.over an 18-month period 485 throat swabs and sputum samples were taken from patients admitted to basra general hospital, iraq, with a clinical diagnosis of pneumonia. most patients (94.0%) had community-acquired pneumonia; 29 (6.0%) had hospital-acquired pneumonia. patients aged < or = 15 years and males had the highest incidence of pneumonia. cases were analysed by type of pneumonia: bronchial (76.3%) versus lobar (23.7%), and primary (81.0%) versus secondary (19.0%). the most common pathogen w ...200717684843
[ecthyma gangrenosum in a patient with non-hodgkin lymphoma].ecthymagangrenosum is a rare disease of the skin that causes the localized necrosis of the skin and subcutaneous fat tissue, leading to the multiple ulcerations surrounded by local hyperaemia. the ulcerations are usually localized in groins, and perianal area. in the majority of cases ecthyma is caused by a pseudomonas aeruginosa sepsis. the disease usually appears in immunocompromized, most frequently hematological patients.200717687947
synthesis and antibacterial activity of some novel chiral fluorophoric biscyclic macrocycles.synthesis of chiral permanent fluorophoric biscyclic macrocycles incorporating anthraquinone and (s)-binol core is described. interestingly, the biscyclic macrocycle 1 exhibited remarkable antibacterial activity against most of the pathogenic bacteria in the tested concentrations as compared to the other three compounds 2, 14 and 17 as well as the test control, tetracycline. further biscyclophanes 1 and 2 exhibited permanent fluorescence sensing property even under highly acidic conditions.200717689076
comparison of real-time polymerase chain reaction and conventional polymerase chain reaction methods for the rapid identification of bacillus anthracis.bacillus anthracis spores have been shown to be one of the most effective biological weapons. for the rapid detection of b. anthracis spores, several genetic markers, including chromosomal and plasmid-based sequences, were studied with polymerase chain reaction (pcr) methods. in the present study, a method using a primer/probe set based on the pxo1-encoded pag gene for the detection of b. anthracis was tested in addition to culture. eight pathological samples (four blood-immersed cotton specimen ...200717691694
apnoea-hypopnoea and mandibular retrusion as uncommon findings associated with proteus syndrome.the aetiology of proteus syndrome (ps) is yet unclear. this disease includes partial gigantism of the hands and/or feet, nevi, hemihypertrophy due to overgrowth of long bones, subcutaneous tumours, macrocephaly, cranial hyperostosis, and pulmonary and renal abnormalities. this case report is about a 17-year-old boy with two uncommon findings associated with ps: apnoea-hypopnoea and mandibular retrusion. a multidisciplinary team was important to provide professional care for this patient. dentist ...200717699709
production of volatile aroma compounds by bacterial strains isolated from different surface-ripened french cheeses.twelve bacterial strains belonging to eight taxonomic groups: brevibacterium linens, microbacterium foliorum, arthrobacter arilaitensis, staphylococcus cohnii, staphylococcus equorum, brachybacterium sp., proteus vulgaris and psychrobacter sp., isolated from different surface-ripened french cheeses, were investigated for their abilities to generate volatile aroma compounds. out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromat ...200717701035
parenting children with proteus syndrome: experiences with, and adaptation to, courtesy stigma.courtesy stigma refers to the stigmatization an unaffected person experiences due to his or her relationship with a person who bears a stigma. parents of children with genetic conditions are particularly vulnerable to courtesy stigma, but little research has been done to explore this phenomenon. the purpose of this study was to investigate the courtesy stigma experiences of parents of children with proteus syndrome (ps) and related overgrowth conditions. thematic analysis of transcripts from 31 ...200717702022
the structure of apo tryptophanase from escherichia coli reveals a wide-open conformation.the crystal structure of apo tryptophanase from escherichia coli (space group f222, unit-cell parameters a = 118.4, b = 120.1, c = 171.2 a) was determined at 1.9 a resolution using the molecular-replacement method and refined to an r factor of 20.3% (r(free) = 23.2%). the structure revealed a significant shift in the relative orientation of the domains compared with both the holo form of proteus vulgaris tryptophanase and with another crystal structure of apo e. coli tryptophanase, reflecting th ...200717704565
[intracranial hypertension in proteus syndrome].proteus syndrome, described for the first time in 1979, is a sporadic congenital poly-malformation syndrome named for its highly variable manifestations. we report the case of a 36-year-old male patient with several malformations including skull hyperostosis and huge frontal sinus hypertrophy compressing the brain. he complained of increasing headache for 5 years. cerebrospinal fluid pressure monitoring revealed severe hypertension. the patient underwent frontoparietal craniectomy, which allowed ...200717706728
[selective action of broad-spectrum antibiotics on intestinal flora in sepsis in rats].to investigate the selective effect of broad-spectrum antibiotics on the intestinal flora in sepsis in rats.200717708836
[first report of parasitism of the trichogramma bruni nagaraja (hymenoptera: trichogrammatidae) in eggs of urbanus proteus (l.) (lepidoptera: hesperiidae) in snap beans (phaseolus vulgaris) ( l.)].aiming to verify the presence of eggs parasitoids of the genus trichogramma, for using in the biological control of insects pests, were collected eggs from long tailed skipper butterfly, a lepidopteran defoliator of snap beans, which presented eggs with parasitism characteristics (dark eggs). the eggs were taken to the laboratory were 44.9% of parasitism was verified. viability was 96.3%, with an average of 6.6 individuals per egg and a sexual ratio of 0.93. male specimens were identified as tri ...200717710338
aggregative adherence of uropathogenic proteus mirabilis to cultured epithelial cells.proteus mirabilis is an important cause of urinary tract infection (uti) in patients with complicated urinary tracts. thirty-five strains of p. mirabilis isolated from uti were examined for the adherence capacity to epithelial cells. all isolates displayed the aggregative adherence (aa) to hep-2 cells, a phenotype similarly presented in llc-mk(2) cells. biofilm formation on polystyrene was also observed in all strains. the mannose-resistant proteus-like fimbriae (mr/p), type i fimbriae and aaf/i ...200717714491
antimicrobial resistance among gram-negative bacilli causing infections in intensive care unit patients in the united states between 1993 and 2004.during the 12-year period from 1993 to 2004, antimicrobial susceptibility profiles of 74,394 gram-negative bacillus isolates recovered from intensive care unit (icu) patients in united states hospitals were determined by participating hospitals and collected in a central location. mics for 12 different agents were determined using a standardized broth microdilution method. the 11 organisms most frequently isolated were pseudomonas aeruginosa (22.2%), escherichia coli (18.8%), klebsiella pneumoni ...200717715376
diversity of microflora in the gut and casts of tropical composting earthworms reared on different substrates.the diversity of fungi, bacteria, yeast, actinomycetes and protozoa were analysed in the gut and casts of eudrilus eugeniae, lampito mauritii, eisenia fetida and perionyx excavatus, both qualitatively and quantitatively as influenced by different feed substrates like clay loam soil, cowdung and pressmud. while actinomycetes (streptomyces albus, s. somaliensis, nocardia asteroides, n. caviae and saccharomonosporia) were not digested by any of these species of worms, protozoa (amoeba proteus, a. t ...200717717992
antimicrobial activity of methanol extract of origanum majorana l. (sweet marjoram).in-vitro microbicidal activity of the methanol extract of origanum majorana l. was tested against seven fungi (fusarium solani, candida albicans, aspergillus niger, a. parasiticus, rhizopus oryzae, rhizoctonia otyzae-sativae and altemaria brassicicola) and six bacteria (bacillus subtilis, b. megaterium, escherichia coil, proteus vulgaris, pseudomonas aeruginosa and staphylococcus aureus). the methanol extract of o. majorana can be used as an effective herbal protectant against different pathogen ...200717718003
in vitro antimicrobial activity of a novel propolis formulation (actichelated propolis).this study compared in vitro activities of actichelated propolis (a multicomposite material obtained with mechano-chemichal activation) and of a hydroalcoholic extract of propolis.200717953601
an unusual case of a ureteric hernia into the sciatic foramen causing urinary sepsis: successfully treated laparoscopically.we present the rare case of a ureteric hernia into the sciatic foramen. the presentation was that of a 59-year-old woman with urinary tract sepsis associated with an acute deterioration of renal function. the hernia was successfully repaired laparoscopically.200717958996
use of in vitro susceptibility and pathogen prevalence data to model the expected clinical success rates of tigecycline and other commonly used antimicrobials for empirical treatment of complicated skin and skin-structure infections.the overall expected coverage for tigecycline and selected antimicrobial regimens based upon prevailing susceptibility rates was evaluated for the empirical therapy of complicated skin and skin-structure infections (csssis). consecutive, non-duplicate bacterial isolates collected from 2000-2005 from patients with documented csssi in 57 medical centres located in the usa (38 centres), france (6 centres), germany (7 centres), italy (3 centres) and spain (3 centres) were used to evaluate the freque ...200717959359
newly delineated syndrome of congenital lipomatous overgrowth, vascular malformations, and epidermal nevi (clove syndrome) in seven patients.we present a series of seven patients who were previously diagnosed with proteus syndrome, but who do not meet published diagnostic criteria for this disorder and whose natural history is distinct from that of proteus syndrome. this newly recognized phenotype comprises progressive, complex, and mixed truncal vascular malformations, dysregulated adipose tissue, varying degrees of scoliosis, and enlarged bony structures without progressive bony overgrowth. we have named this condition congenital l ...200717963221
species interactions in mixed-community crystalline biofilms on urinary catheters.previous experimental investigations of the crystalline biofilms that colonize and block urinary catheters have focussed on their formation by pure cultures of proteus mirabilis. in the urine of patients undergoing long-term catheterization, p. mirabilis is commonly found in mixed communities with other urinary tract pathogens. little is known about the effect that the other species have on the rate at which p. mirabilis encrusts catheters. in the present study, a set of data on the nature of th ...200717965358
polyamines in bacteria: pleiotropic effects yet specific mechanisms.extensive data in a wide range of organisms point to the importance of polyamine homeostasis for growth. the two most common polyamines found in bacteria are putrescine and spermidine. the investigation of polyamine function in bacteria has revealed that they are involved in a number of functions other than growth, which include incorporation into the cell wall and biosynthesis of siderophores. they are also important in acid resistance and can act as a free radical ion scavenger. more recently ...200717966408
[epidemiology and treatment of acute prostatitis after prostatic biopsy].acute prostatitis is the main complication of prostatic biopsies (pb) and sometimes requires hospitalisation and appropriate antibiotic therapy. this study evaluated the pathogens responsible and proposes a statistically adapted empirical antibiotic therapy.200717969797
effect of natural preservatives on the growth of histamine producing bacteria.present study deals with the hampering of the growth of histamine producing bacteria (hpb), by using nacl and spices which are easily available and cheaper cost wise. for this experiment, four strains of hpb viz. vibrio parahaemolyticus, bacillus cereus, pseudomonas aeruginosa and proteus mirabilis were tested against 1 to 10% concentrations of nacl and 1 to 5% concentrations of natural preservatives (turmeric, ginger and garlic) in a basal medium. hpb showed different growth rates at different ...200717915763
cowden syndrome.cowden syndrome (cs), due to germline mutations of the pten tumor-suppressor gene, is an often overlooked cancer predisposition syndrome associated with an increased risk of breast, thyroid, and endometrial cancers, as well as benign manifestations. germline pten mutations also are associated with syndromes that have not been historically connected to an increase in risk for malignancy. these disorders include bannayan-riley-ruvalcaba syndrome (brrs), proteus syndrome (ps), and proteus-like synd ...200717920899
bacterial contaminations of informally marketed raw milk in ghana.summary200717925843
brain abscess in infants and children: a retrospective study of 107 patients in northeast thailand.to review the clinical manifestation, predisposing factor location of abscess, causative organism, treatment, and outcome of brain abscess.200717926990
[phosphatase activity in amoeba proteus at ph 9.0].in the free-living amoeba amoeba proteus (strain b), after paag disk-electrophoresis of the homogenate supernatant, at using 1-naphthyl phosphate as a substrate and ph 9.0, three forms of phosphatase activity were revealed; they were arbitrarily called "fast", "intermediate", and "slow" phosphatases. the fast phosphatase has been established to be a fraction of lysosomal acid phosphatase that preserves some low activity at alkaline ph. the question as to which particular class the intermediate p ...200717933343
regulatory circuit based on autogenous activation-repression: roles of c-boxes and spacer sequences in control of the pvuii restriction-modification system.type ii restriction-modification (r-m) systems comprise a restriction endonuclease (rease) and a protective methyltransferase (mtase). after r-m genes enter a new cell, mtase must appear before rease or the chromosome will be cleaved. pvuii and some other r-m systems achieve this delay by cotranscribing the rease gene with the gene for an autogenous transcription activator (the controlling or 'c' protein c.pvuii). this study reveals, through in vivo titration, that c.pvuii is not only an activat ...200717933763
structure of a glucosyl phosphate-containing o-polysaccharide of proteus vulgaris o42.an o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of proteus vulgaris o42 and studied by sugar and methylation analyses along with 1h, 13c and 31p nmr spectroscopy. the following structure of the polysaccharide having a linear pentasaccharide phosphate repeating unit was established: -->3)-alpha-l-fucpnac4ac-(1-->4)-alpha-d-glcp-1-p-(o-->4)-alpha-d-glcpnac-(1-->3)-alpha-l-fucpnac4ac-(1-->3))-alpha-d-glcpnac6ac-(1--> where the degree of o-acetylation is approxima ...200717936254
antimicrobial susceptibility of klebsiella spp. and proteus spp. from various organ systems of horses, dogs and cats as determined in the bft-germvet monitoring program 2004-2006.a total of 120 isolates of klebsiella spp. and proteus spp. collected from horses and small animals (dogs and cats) were screened for their susceptibility to 24 different antimicrobial agents. klebsiella spp. were included from infections of the genital tract (gt) of horses (36 isolates) and the urinary/genital tract (ugt) from dogs and cats (17 isolates), while proteus spp. were from small animal (dogs and cats) infections of the ugt (37 strains) and the skin (incl. ear/mouth) (30 isolates). in ...200717939455
proteus syndrome in the aetiology of carpal tunnel syndrome. 200717939492
anti-amebic antibody activity in patients, determined with antigens prepared from virulent parasites (indirect hemagglutination assay and enzyme-linked immunosorbent assay).the serology of amebiasis is affected by low sensitivity and specificity.200717939629
clinical epidemiology of ciprofloxacin-resistant proteus mirabilis isolated from urine samples of hospitalised patients.this study investigated the clinical characteristics of ciprofloxacin-resistant proteus mirabilis isolates from urine samples associated with nosocomial infection or colonisation, and identified the risk-factors for ciprofloxacin resistance. data for patients with ciprofloxacin-resistant p. mirabilis isolates (n=13) were compared with those for randomly selected patients with ciprofloxacin-susceptible p. mirabilis isolates (n=40) who were matched by temporal occurrence as control patients. the m ...200717850340
bacteriology of chronic suppurative otitis media--a multicenter study.bacterial predominance and antibiotic sensitivity have changed over time, making continuous and periodic surveillance necessary in guiding appropriate antibacterial therapy.200717851935
mannose-resistant proteus-like and p. mirabilis fimbriae have specific and additive roles in p. mirabilis urinary tract infections.proteus mirabilis is an important uropathogen that can cause complicated urinary tract infections (uti). it produces several types of fimbriae, including mannose-resistant proteus-like (mr/p) fimbriae and p. mirabilis fimbriae (pmf). previously, we determined that these fimbriae affect the ability of p. mirabilis to colonize the urinary tract. the objective of this study was to analyse the effect of the simultaneous lack of p. mirabilis mr/p and pmf fimbriae in uti pathogenesis. a double mutant ...200717854474
bacterial defluorination of 4-fluoroglutamic acid.fluorinated amino acids are used as enzyme inhibitors, mechanistic probes and in the production of pharmacologically active peptides. because enantiomerically pure 4-fluoroglutamate is difficult to prepare, the selective degradation of the l-isomer is a potentially convenient method of obtaining d-4-fluoroglutamate from the racemate. in this paper, we describe our investigations on the degradation of 4-fluoroglutamate by bacteria. fluoride ion was detected in resting-cell cultures of a number of ...200717901951
pattern of pathogens in ear discharge of hiv-infected children in nnewi, southeast nigeria.this is a descriptive, prospective, hospital-based study designed to determine the prevalent organisms in ear infections and their antimicrobial susceptibility patterns in hiv-infected children compared to age-and sex-matched hiv sero-negative patients.200717902505
purification, crystallization, x-ray diffraction analysis and phasing of an engineered single-chain pvuii restriction endonuclease.the restriction endonuclease pvuii from proteus vulgaris has been converted from its wild-type homodimeric form into the enzymatically active single-chain variant scpvuii by tandemly joining the two subunits through the peptide linker gly-ser-gly-gly. scpvuii, which is suitable for the development of programmed restriction endonucleases for highly specific dna cleavage, was purified and crystallized. the crystals diffract to a resolution of 2.35 a and belong to space group p4(2), with unit-cell ...200717909283
septic postpartum uterine inversion.puerperal uterine inversion is an uncommon but life threatening obstetrical emergency. a 26-year-old woman, para six, was referred from a peripheral hospital seven days after delivery, with a mass protruding per vaginum. complete uterine inversion had occurred after delivery of baby and placenta. she was resuscitated and her genital infection was treated. she had a vaginal hysterectomy upon request. her postoperative recovery was uneventful. poor management of the third stage of labour is a comm ...200717909681
production of l-carnitine by secondary metabolism of bacteria.abstract: the increasing commercial demand for l-carnitine has led to a multiplication of efforts to improve its production with bacteria. the use of different cell environments, such as growing, resting, permeabilized, dried, osmotically stressed, freely suspended and immobilized cells, to maintain enzymes sufficiently active for l-carnitine production is discussed in the text. the different cell states of enterobacteria, such as escherichia coli and proteus sp., which can be used to produce l- ...200717910757
antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent.the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ...200717913083
[the antibacterial activity of fluoroquinolones against gram-negative bacteria isolated from gifu prefecture (2005)].we investigated the susceptibility to 6 fluoroquinolones against 433 strains of gram-negative bacteria isolated from 6 medical facilities in gifu prefecture between january and september in 2005, determined by the agar dilution methods in according with the japan society of chemotherapy. we also investigated the correlation between the degree of resistance to fluoroquinolones and the amino acid substitutions in the quinolone resistance-determining region (qrdr). the tested clinical isolates were ...200717867488
frequency of urinary tract infection in catheterized dogs and comparison of bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with urinary tract infections.to determine frequency of urinary tract infections (utis) in catheterized dogs that had intervertebral disk disease (ivdd) or disease other than ivdd and compare bacterial culture and susceptibility testing results for catheterized and noncatheterized dogs with utis.200717867973
synthesis and antimicrobial activity of some novel nucleoside analogues of adenosine and 1,3-dideazaadenosine.a number of nucleoside analogues have been synthesized and evaluated for their antibacterial and antifungal activities against staphylococcus aureus, group d streptococcus, pseudomonas aeruginosa, proteus spp., salmonella spp., aspergillus fumigatus, penicillium marneffei, candida albicans, cryptococcus neoformans, and mucor spp. the compounds 1, 4, and 6 emerged as potent antibacterial agents with mic values of 0.75, 0.38, and 0.19 microm, respectively, against group d streptococcus. further, t ...200717890082
breast abscess bacteriologic features in the era of community-acquired methicillin-resistant staphylococcus aureus epidemics.increasing rates of community-acquired methicillin-resistant staphylococcus aureus (mrsa) infections have also affected the microbial profile of breast abscesses.200717891866
the type iii secretion system of proteus mirabilis hi4320 does not contribute to virulence in the mouse model of ascending urinary tract infection.the gram-negative enteric bacterium proteus mirabilis is a frequent cause of urinary tract infections (utis) in individuals with long-term indwelling catheters or with complicated urinary tracts. the recent release of the p. mirabilis strain hi4320 genome sequence has facilitated identification of potential virulence factors in this organism. genes appearing to encode a type iii secretion system (ttss) were found in a low gc-content pathogenicity island in the p. mirabilis chromosome. this islan ...200717893161
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