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modeling and designing a proteomics application on proteus.biomedical applications, such as analysis and management of mass spectrometry proteomics experiments, involve heterogeneous platforms and knowledge, massive data sets, and complex algorithms. main requirements of such applications are semantic modeling of the experiments and data analysis, as well as high performance computational platforms. in this paper we propose a software platform allowing to model and execute biomedical applications on the grid.200515924180
characterization of a cdna of peroxiredoxin ii responding to hydrogen peroxide and phagocytosis in amoeba proteus.phagocytic cells have defense systems against reactive oxygen species generated as the first non-specific defense mechanism against invading pathogens or microorganisms. we cloned a cdna encoding a 21.69-kda protein in amoeba proteus homologous to 2-cys peroxiredoxin (prx-ap). in the disk inhibition assay using h2o2 as an oxidizing agent, escherichia coli overproducing prx-ap showed better viability than did e. coli transformed with pbluescript ii sk for control. monoclonal antibodies (mab) prod ...200515926998
sternal split approach to the cervicothoracic junction in children.we present a descriptive case series outlining the surgical technique and outcome in six patients managed with a combined anterior neck and sternal splitting approach.200515928540
preventive and therapeutic administration of an indigenous lactobacillus sp. strain against proteus mirabilis ascending urinary tract infection in a mouse model.probiotics are increasingly being considered as non-pharmaceutical and safe potential alternatives for the treatment and prevention of a variety of pathologies including urinary tract infections. these are the most common infections in medical practice and are frequently treated with antibiotics, which have generated an intense selective pressure over bacterial populations. proteus mirabilis is a common cause of urinary tract infections in catheterised patients and people with abnormalities of t ...200515928974
organisms causing urinary tract infection in pediatric patients at ayub teaching hospital abbottabad.urinary tract infection (uti) is common in pediatric practice and an important cause of morbidity and mortality in children. infected urine stimulates an immunological and inflammatory response leading to renal injury and scarring, ultimately leading to end stage renal failure. the present study identifies the micro organisms causing urinary tract infection in children presenting with uti at ayub teaching hospital, abbottabad.200515929535
plasmid-mediated, inducible ampc beta-lactamase (dha-1)-producing enterobacteriaceae at a korean hospital: wide dissemination in klebsiella pneumoniae and klebsiella oxytoca and emergence in proteus mirabilis.the aim of the study was to investigate the phenotypic and genetic characteristics of recently emerging cefoxitin-resistant and induction-positive isolates of escherichia coli, klebsiella species, and proteus mirabilis. strains of enterobacteriaceae were isolated at a korean tertiary care hospital between june and december 2002. induction was tested using cefoxitin and aztreonam disks, the bladha allele was detected by pcr, and pulsed-field gel electrophoresis (pfge) patterns were also analyzed. ...200515936167
dentomaxillofacial imaging in proteus syndrome.proteus syndrome is a rare condition that involves atypical growth of the bones, skin and head and a variety of other symptoms. only a few authors have reported on the craniofacial manifestations so far. the authors present a case of a 7-year-old girl with proteus syndrome in which the facial skeleton showed unilateral overgrowth. the analysis of the radiological evaluation revealed a bialveolar prognathism, a skeletal class iii, a dolicocephalic growth pattern and a left convex face scoliosis. ...200515961602
spread of novel expanded-spectrum beta-lactamases in enterobacteriaceae in a university hospital in the paris area, france.in 2002, 28 non-duplicate enterobacterial isolates producing extended-spectrum beta-lactamases (esbls) were collected from infected patients at the bicêtre hospital in paris, france. escherichia coli was the predominant esbl-positive enterobacterial species, comprising ten (36%) of the isolates. ctx-m enzymes (ctx-m-3, ctx-m-10, ctx-m-14 and ctx-m-15) were produced by 11 (39%) of the isolates (six e. coli, two enterobacter cloacae, one enterobacter aerogenes, one proteus mirabilis and one citrob ...200515966981
first description of dha-1 ampcbeta-lactamase in proteus mirabilis.this report describes the first occurrence of the dha-1 ampcbeta-lactamase gene in proteus mirabilis. the organism was isolated from the vaginal flora of a pregnant woman in a french hospital. the dha-1 beta-lactamase gene was identified on the basis of phenotypic characteristics, pcr amplification and sequencing. antagonism between cefoxitin and the other cephalosporins suggested inducible production of the dha-1 enzyme.200515966982
in-vitro antibacterial, antifungal and cytotoxic properties of sulfonamide--derived schiff's bases and their metal complexes.a series of new antibacterial and antifungal schiff's bases derived from sulfonamides, as well as their transition metal complexes incorporating cobalt (ii), copper (ii), nickel (ii) and zinc (ii) were synthesized, characterized and screened for their in-vitro antibacterial activity against six gram-negative (escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi and shigella dysentriae) and four gram-positive (bacillus cereus, corynebacterium diphth ...200515968823
[antibiotic resistance transformation in community-acquired urinary infections].to detect the prevalence of uropathogens in community-acquired urinary tract infection in our environment, and the degree of sensitivity to antibiotics used as empirical treatment.200515970133
noninvasive biophotonic imaging for monitoring of catheter-associated urinary tract infections and therapy in mice.urinary tract infections (utis) are among the most common bacterial infections acquired by humans, particularly in catheterized patients. a major problem with catheterization is the formation of bacterial biofilms on catheter material and the risk of developing persistent utis that are difficult to monitor and eradicate. to better understand the course of utis and allow more accurate studies of in vivo antibiotic efficacy, we developed a catheter-based biofilm infection model with mice, using bi ...200515972473
antimicrobial susceptibility pattern of blood isolates from a teaching hospital in north india.bloodstream infections are associated with significant patient morbidity and mortality worldwide. in this study, we examined antimicrobial susceptibility patterns by reviewing the data on 5,704 blood samples that were collected from patients with fever/sepsis admitted to government medical college and hospital, chandigarh, india, over a period of 1 year from august 2003 to july 2004. among the 567 qualifying samples, pseudomonas aeruginosa (19.75%), escherichia coli (15.17%), klebsiella pneumoni ...200515973011
bioactivity guided isolation of antimicrobial compounds from lythrum salicaria.lythrum salicaria extracts showed activity against the phytopathogenic fungus cladosporium cucumerinum and activity against the bacteria staphylococcus aureus, proteus mirabilis and microccocus luteus. bioautography on thin-layer chromatograms was used to isolate the two antifungal triterpenoids oleanolic and ursolic acid. the hexahydroxydiphenoyl ester vescalagin was isolated as active principle of the antibacterial activity. furthermore, the flavon-c-glucosides vitexin, isovitexin, orientin an ...200515975734
structure of the o-polysaccharide of proteus mirabilis oc (ccug 10702) from a new proposed proteus serogroup o75.a neutral o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of proteus mirabilis oc (ccug 10702) and studied by sugar and methylation analyses and (1)h and (13)c nmr spectroscopy. the following structure of the tetrasaccharide repeating unit of the polysaccharide was established: [structure: see text]. based on the unique structure of the o-polysaccharide and serological data, we propose classifying p. mirabilis oc (ccug 10702) into a new separate proteus serogroup ...200515979596
proteus mirabilis bloodstream infections: risk factors and treatment outcome related to the expression of extended-spectrum beta-lactamases.bloodstream infection (bsi) due to proteus mirabilis strains is a relatively uncommon clinical entity, and its significance has received little attention. this study was initiated to evaluate risk factors and treatment outcome of bsi episodes due to p. mirabilis producing extended-spectrum beta-lactamases (esbls). twenty-five bsi episodes caused by p. mirabilis occurred at our hospital (ospedale di circolo e fondazione macchi, varese, italy) over a 7.5-year period. phenotypic and molecular metho ...200515980325
peptides selected for binding to a virulent strain of haemophilus influenzae by phage display are bactericidal.nontypeable haemophilus influenzae (nthi) is an obligate parasite of the oropharynx of humans, in whom it commonly causes mucosal infections such as otitis media, sinusitis, and bronchitis. we used a subtractive phage display approach to affinity select for peptides binding to the cell surface of a novel invasive nthi strain r2866 (also called int1). over half of the selected phage peptides tested were bactericidal toward r2866 in a dose-dependent manner. five of the clones encoded the same pept ...200515980377
proteus: identifying signatures in protein termini.proteus (protein terminus) is a web-based tool for the identification of short linear signatures in protein termini. it is based on a position-based search method for revealing short signatures in termini of all proteins. the initial step in proteus development was to collect all signature groups (sigs) based on their relative positions at the termini. the initial set of sigs went through a sequential process of inspection and removal of sigs, which did not meet the attributed statistical thresh ...200515980469
clinical management and outcome of childhood lung abscess: a 16-year experience.in order to evaluate the clinical manifestations, management and outcome of childhood lung abscess, a retrospective chart review of 27 pediatric patients with international classification of diseases, ninth revision-clinical modification (icd-9 cm) code of 503.1 (lung abscess) from august 1987 to august 2003 was conducted. among the 27 patients (14 males and 13 females), 30% (8/27) were primary lung abscess and 70% (19/27) had underlying chronic diseases (secondary lung abscess). the predisposin ...200515986068
selective iga immune unresponsiveness to proteus mirabilis fumarate reductase a-chain in rheumatoid arthritis.to determine if selective immune unresponsiveness to microbial antigens is associated with predisposition to rheumatoid arthritis (ra).200515996053
aetiology of catheter-associated bacteriuria in lagos university teaching hospital.a prospective study to determine the causative agents of catheter-related bacteriuria was carried out on 99 patients with median age of 55 years, on admission in the oncology, paediatric surgery and obstetric and gynaecology wards of the lagos university teaching hospital. there was significant bacteriuria in seventy-one (72%) of the 99 urine samples. all catheter urine samples were contaminated by day 5 gram-negative bacilli were predominant (89.3%) of which pseudomonas aeruginosa (26.2%) was t ...200515997255
[long-term efficacy of trus-guided transperineal needle aspiration and irrigation on persistent hematospermia].to evaluate the long-term efficacy of transrectal ultrasonography (trus)-guided transperineal needle aspiration and irrigation for persistent hematospermia.200515999493
ampc disk test for detection of plasmid-mediated ampc beta-lactamases in enterobacteriaceae lacking chromosomal ampc beta-lactamases.although plasmid-mediated ampc beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. these enzymes are typically produced by isolates of escherichia coli, klebsiella spp., proteus mirabilis, and salmonella spp. and are associated with multiple antibiotic resistance that leaves few therapeutic options. plasmid-mediated ampc beta-lactamases have been associated with false in vitro susceptibility to cephalosporins. many ...200516000421
[xanthogranulomatous pyelonephritis: presentation of an unusual case].xanthogranulomatous pyelonephritis is a morphologically and clinically unique manifestation of chronic pyelonephritis with the formation of pus or granulomas. the most frequent predisposing factors for the development of xanthogranulomatous pyelonephritis are urinary obstruction (e. g., stones, tumors, congenital anomalies and functional impairment) and infection of the collecting system.200516001341
evaluation of cd4(+)/cd8(+) status and urinary tract infections associated with urinary schistosomiasis among some rural nigerians.data on urinary schistosomiasis in nigeria are mainly epidemological. the knowledge of co-infections of urinary schistosomiasis and other pathogens are important epidemiological tools for the control and health benefits of the rural dwellers. the granulomatous reactions in urinary schistosomiasis is cd4(+) dependent. the cd8(+) is cytotoxic to parasites and it is activated by cd4(+). these parameters therefore participate in the immune responses to urinary schistosomiasis.200516006219
analysis of pten gene mutations in korean patients with cowden syndrome and polyposis syndrome.pten (phosphatase and tensin homologue deleted in chromosome 10) is a candidate tumor suppressor gene. mutations of this gene are responsible for pten hamartoma tumor syndromes, including cowden syndrome, bannayan-riley-ruvalcaba syndrome, proteus syndrome, and proteus -like syndromes. recently, pten mutations were identified in several human neoplasms. we analyzed the dna of various organs and lesions in korean patients with cowden syndrome, their family members, and patients with familial aden ...200516007494
etiological and clinical patterns of childhood urolithiasis in iraq.this study was conducted to evaluate the etiological and clinical characteristics of urolithiasis in iraqi children. from 1999 to 2004, 204 children with renal calculi were evaluated. the age range of the patients was 4 months to 14 years, 61.3% of the patients were under 5 years. male to female ratio was 2.8:1. the mean age at onset of symptoms was 3.2 years, and stone disease was diagnosed at a mean of 3.5 years. hematuria (44.6%) and pain (28.4%) were the main clinical presentation. of the 20 ...200516010596
proteus syndrome: an update.proteus syndrome is a complex disorder consisting variably of disproportionate, asymmetric overgrowth of body parts; cerebriform connective tissue nevi; epidermal nevi; vascular malformations of the capillary, venous, and lymphatic types; and dysregulated adipose tissue. serious complications may ensue, such as pulmonary embolism, cystic lung disease, and various neoplasms. somatic mosaicism, lethal in the nonmosaic state, is the best working hypothesis. although proteus syndrome data are consis ...200516010681
[synthesis and antibacterial activity of 3-(5-substituted phenyl-[1,3,4] oxadiazole-2-yl-methylenethio)-5-pyridin-3-yl-[1,2,4] triazole-4-yl-amines].to study on synthesis and antibacterial activity evaluation of polyheterocycles.200516011262
swarmer cell differentiation in proteus mirabilis.under the appropriate environmental conditions, the gram-negative bacterium proteus mirabilis undergoes a remarkable differentiation to form a distinct cell type called a swarmer cell. the swarmer cell is characterized by a 20- to 40-fold increase in both cell length and the number of flagella per cell. environmental conditions required for swarmer cell differentiation include: surface contact, inhibition of flagellar rotation, a sufficient cell density and cell-to-cell signalling. the different ...200516011745
molecular characterization of multidrug-resistant proteus mirabilis isolates from retail meat products.sixty-four multidrug-resistant isolates of proteus mirabilis were collected from retail meat products in oklahoma. the isolates showed four different patterns of antibiotic resistance based on their resistant phenotype and genotypes. most of these isolates were resistant to ampicillin, tetracycline, gentamycin, and kanamycin. class 1 integrons were detected as a common carrier of the antibiotic-resistant genes, such as aada1, aadb, and aada2. a few isolates (9%) contained class 2 integrons with ...200516013378
colouterine fistula mimicking pyometrium--diagnosis established with multi-detector computed tomography. 200516022134
pyrrolobenzodiazepine dimers: novel sequence-selective, dna-interactive, cross-linking agents with activity against gram-positive bacteria.pyrrolo[2,1-c][1,4]benzodiazepine (pbd) dimers are synthetic sequence-selective interstrand dna minor-groove cross-linking agents developed from anthramycins. we investigated the antibacterial activity of three dimers, sjg-136, drg-16 and elb-21, which differ in the structure of the pbd monomeric unit and the length of the linker region between the two identical pbd monomers.200516024592
[species-specific detection of proteus vulgaris and proteus mirabilis by the polymerase chain reaction].sets of primers for the species-specific detection of p. mirabilis and p. vulgaris by the polymerase chain reaction (pcr) were developed. as targets for these primers beta-lactamase and 16s rrna gene fragments were chosen on the basis of the multiple leveling of the sequences of the dna of all known p. mirabilis and p. vulgaris isolates. for differential detection oligonucleotides were selected in such a way that primers, specific for p. vulgaris, contained the non-paired nucleotide for p. mirab ...200516028510
preclinical evaluation of rapeseed, raspberry, and pine bark phenolics for health related effects.rapeseed, raspberry, and pine bark are promising bioactive sources of plant phenolics selected from among ca. 100 previously screened plant materials for in vitro preclinical evaluation of health related effects. phenolic extracts and isolated fractions of the selected materials were investigated for antioxidant, antimicrobial, antiinflammatory, and antimutagenic properties as well as for cell permeability. it was shown that rapeseed and pine bark phenolics and raspberry anthocyanins were good o ...200516028975
extended-spectrum beta-lactamases.the first beta-lactamase was identified in an isolate of escherichia coli in 1940. to date, there are >130 tem-type and >50 sulfhydryl variable (shv)-type beta-lactamases, mainly in e. coli, klebsiella pneumoniae, and proteus mirabilis but also in other members of the enterobacteriaceae family and in some nonenteric organisms, such as acinetobacter species. the incidence of expanded-spectrum beta-lactamases (esbls) varies, depending on which area of the globe the isolates originate from. esbls r ...200516032564
recalcitrant scoliosis in proteus syndrome.proteus syndrome is a rare congenital condition with various clinical features such as hemihypertrophy, macrodactyly, subcutaneous masses, brain-like hyperplasia of the soles and/or palms, epidermal naevi and scoliosis with other mesodermal malformations. multifocal overgrowth can affect various tissues causing severe functional and cosmetic disability, but intellectual and language development are mostly normal. orthopaedic problems include macrodactyly, hindfoot deformity, limb length inequali ...200516035717
antimicrobial susceptibility patterns of contemporary pathogens from uncomplicated urinary tract infections isolated in a multicenter italian survey: possible impact on guidelines.during 2004 four italian laboratories assessed the prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in female out-patients. a total of 600 urine samples from individuals aged 18-65 were studied. the overall prevalence of escherichia coli was 85.3%. klebsiella pneumoniae, staphylococcus saprophyticus, proteus mirabilis, enterococcus faecalis and other rarer species were far less represented. determination of the antibiotic susceptibility pattern of th ...200516038517
antibacterial and antioxidant activities of ethanol extract from paullinia cupana mart.the antibacterial and antioxidant activity of the ethanol extract from paullinia cupana var. sorbilis mart. (sapindaceae) seeds, commonly called guarana, was assessed towards selected bacteria as well as in different antioxidant models. the extract, at a concentration between 16 and 128 microg/ml, showed a significant antibacterial effect expressed as minimum inhibitory concentration (mic) against both gram-negative and gram-positive bacteria. in particular, pseudomonas aeruginosa (mic=16 microg ...200516040216
tigecycline.new antimicrobial agents are urgently needed for clinical use due to the increasing prevalence and spread of multidrug-resistant bacteria that are commonly responsible for serious and life-threatening diseases. the need to develop new agents that effectively overcome existing mechanisms of resistance displayed by bacteria resistant to currently available drugs has become paramount. tigecycline, the first in a new class of antimicrobials, the glycylcyclines, is an analogue of minocycline with add ...200516040625
application of electrospray ionization with fourier transform ion cyclotron resonance mass spectrometry for structural screening of core oligosaccharides from lipopolysaccharides of the bacteria proteus.electrospray ionization with fourier transform ion cyclotron resonance mass spectrometry (esi-fticrms) was used for screening and structural elucidation of core oligosaccharides isolated from lipopolysaccharides of bacteria of the genus proteus. mass spectra allowed the determination of the molecular masses with high accuracy and the estimation of the chemical heterogeneity of the samples. they did not, however, provide sufficient information to identify structural details of the branched oligos ...200516041825
[hydrolytic activity of marine bacteria associated with the mussel mytilus trossulus].the ability to produce hydrolytic enzymes--dnases, rnases, alkaline phosphatases, chitinases has been studied in 316 strains of bacteria isolated from mussel mytilus trossulus. strains of vibrio spp. produced all the spectrum of examined enzymes, bacteria of pseudoalteromonas genus produced alkaline phosphatase and dnase. chitin-degrading enzymes were found in vibrio and cytophaga--flavobacterium--bacteroides group. bacillus and vibrio spp. strains produced most actively rnases. the presence of ...200515765877
malignant mimickers: chronic bacterial and fungal infections of the larynx.chronic infections of the larynx are notorious "copycats" of squamous cell carcinoma. patients typically present with a historical picture and symptoms identical to those seen in a neoplastic setting: dyspnea, hoarseness, odynophagia, weight loss, and a history of tobacco and alcohol abuse. historically, these patients were subject to an extensive resection for what was in reality a benign disease. a better understanding and awareness of these conditions has reinforced the need for a direct lary ...200515766861
antimicrobial activity of sesquiterpenes from the essential oil of juniperus thurifera.chemical analysis of the essential oil of juniperus thurifera wood led to the identification of fifty-eight sesquiterpene compounds, of which twenty-eight have been isolated, two of them being new natural cedrane derivatives (1 and 2). their structures were established on the basis of 1d nmr and 2d nmr spectra. the antimicrobial activities of different pure compounds of this oil were tested against nine microorganisms (gram-positive and gram-negative bacteria, and yeasts). seven of the tested co ...200515678376
structure and serological studies of the o-polysaccharide of proteus penneri 75 epitopes and subgroups of proteus serogroup o73.the o-specific polysaccharide of the lipopolysaccharide of proteus penneri strain 75 consists of tetrasaccharide-ribitol phosphate repeating units and resembles ribitol teichoic acids of gram-positive bacteria. the following structure of the polysaccharide was elucidated by chemical methods and 1h and 13c nmr spectroscopy: [structure in text] where rib-ol is ribitol. serological studies with polyclonal antisera showed that the same structure of the o-polysaccharide occurred in two strains: p. pe ...200515681143
[enzymatic resistance to beta lactam antibiotics within the genus proteus and evaluation of proteus mirabilis phenotypes and genotypes for resistance to third- and fourth-generation cephalosporins].the aim of this study was to evaluate betalactam resistance within the genus proteus and characterize the betalactamases responsible for this resistance.200515757582
interleukin-8 response in cells from the human urinary tract induced by lipopolysaccharides of proteus mirabilis o3 and o18.proteus mirabilis is a common pathogen associated mainly with complicated urinary tract infections and sometimes with septicemia. there is great serological diversity of the microorganism. while p. mirabilis o3 is commonly found in patients with infections, the serotype o18 rarely occurs. the o18 lipopolysaccharide contains a phosphocholine substitute, which makes it unique among proteus strains. to explain different clinical significance of the strains we evaluated the biological activity of th ...200515758809
(1)h nmr spectroscopy in the diagnosis of pseudomonas aeruginosa-induced urinary tract infection.the utility of (1)h nmr spectroscopy is suggested and demonstrated for the diagnosis of pseudomonas aeruginosa in urinary tract infection (uti). the specific property of p. aeruginosa of metabolizing nicotinic acid to 6-hydroxynicotinic acid (6-ohna) is exploited. the quantity of 6-ohna produced correlates well with the viable bacterial count. other common bacteria causing uti such as escherichia coli, klebsiella pneumonia, enterobacter aerogenes, acinetobacter baumanii, proteus mirabilis, citro ...200515759292
chlorine covers on living bacteria: the initial step in antimicrobial action of active chlorine compounds.although active chlorine compounds are well-known antimicrobial agents in human medicine, their initial steps of action have not been completely clarified. using n-chlorotaurine (nct), an endogenous mild representative, we observed persisting oxidation capacity affixed to bacteria. it was the aim of this study to investigate this 'chlorine cover'.200515761074
synthesis of some 1-[(n, n-disubstituted thiocar bamoylthio)acetyl]-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives and investigation of their antibacterial and antifungal activities.fourteen new 1-[(n, n-disubstituted thiocarbamoylthio)acetyl]-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives (7a-n) were synthesised by reacting 1-(chloroacetyl)-3-(2-thienyl)-5-aryl-2-pyrazolines (5a-g) and appropriate sodium salts of n, n-disubstituted dithiocarbamoic acids (6a, b). the structures of the synthesised compounds were confirmed by elemental analyses, uv, ir, (1)h-nmr and fab(+)-ms spectral data. their antibacterial activities against proteus vulgaris (nrrl b-123), escherichia coli ...200515765490
evaluation of antimicrobial activity of beta-lactam antibiotics using etest against clinical isolates from 60 medical centres in japan.an antimicrobial resistance surveillance study was carried out in 60 medical centres across japan. resistance to piperacillin was 10.8% in clinical isolates of escherichia coli, while 1.3% or fewer isolates were resistant to other beta-lactams. klebsiella spp. were more susceptible to imipenem, cefepime and cefpirome. isolates of enterobacter spp., citrobacter spp., indole-positive proteus and serratia spp. were susceptible to imipenem, cefepime and cefpirome, while acinetobacter spp. were most ...200515784308
the antimicrobial activity of extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent.the antimicrobial activity and the mic values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. at least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus vulgaris, salmonella ...200515787241
[resistance of catheter-associated urinary tract infections to antibacterials].catheter-associated urinary tract infections (cauti) are the most common nosocomial infections. the worldwide data show the increasing resistance to conventional antibiotics among urinary tract pathogens.200515790045
incidental detection of purulent fluid in kidney at percutaneous nephrolithotomy for branched renal calculi.some patients undergoing percutaneous nephrolithotomy (pcnl) have purulent fluid in the pelvicaliceal system at the time of puncture, although preoperative features are not suggestive of infection. we report the management and outcome of 19 such patients.200515798405
subset of individuals with autism spectrum disorders and extreme macrocephaly associated with germline pten tumour suppressor gene mutations.the genetic aetiology of autism remains elusive. occasionally, individuals with cowden syndrome (a cancer syndrome) and other related hamartoma disorders such as bannayan-riley-ruvalcaba syndrome, proteus syndrome, and proteus-like conditions, are characterised by germline pten mutations, and may have neurobehavioural features resembling autism as well as overgrowth and macrocephaly. therefore, we undertook pten gene mutation analysis in 18 subjects mainly prospectively ascertained with autism s ...200515805158
molecular diversity of proteus mirabilis isolates producing extended-spectrum beta-lactamases in a french university hospital.between february 1997 and december 2002, 3340 hospitalised patients yielded samples positive for proteus mirabilis, of whom 45 (1.3%) were colonised/infected by p. mirabilis producing extended-spectrum beta-lactamases (esbls). the gross incidence of patients colonised/infected by esbl-producing p. mirabilis was 1.61/10(5) days of hospitalisation, with 20% of isolates being collected from patients in urology wards, most frequently (53.3%) from urine samples. seventeen (37.7%) of the 43 isolates w ...200515819867
ossicular discontinuity and exostoses in proteus syndrome: a case report.proteus syndrome (ps) is a rare hamartomatous disorder characterized by mosaic overgrowth of multiple tissues that manifests early in life and is progressive. the presence of unilateral external auditory canal exostoses in a patient who is not a swimmer or surfer is suggestive of ps. however, hearing loss is not a typical feature. here, we describe exostoses and ossicular discontinuity with conductive hearing loss in a patient with ps. the treatment consisted of canalplasty and ossicular chain r ...200515825577
[severe community-acquired pneumonia due to proteus mirabilis]. 200515826556
childhood parapneumonic pleural effusion in enugu.to review the epidemiological pattern and clinical features, management and outcome of childhood parapneumonic pleural effusion in enugu.200515827593
survey of the susceptibility of urinary isolates to antibacterial agents in 2003.in 2003, 826 uropathogenic strains were obtained from the urine of our patients. we assessed their activities to antibacterial agents based on the minimal inhibitory concentration (mic) breakpoints recommended by the national committee for clinical standards (nccls). methicillin-resistant strains accounted for about 70% of staphylococcus aureus and staphylococcus epidermidis. the most common gram-positive species was enterococcus faecalis (15.6%). enterococcus faecium was the most resistant of t ...200515729488
identification and functional characterisation of genes and corresponding enzymes involved in carnitine metabolism of proteus sp.enzymes involved in carnitine metabolism of proteus sp. are encoded by the cai genes organised as the caitabcdef operon. the complete operon could be sequenced from the genomic dna of proteus sp. amino acid sequence similarities and/or enzymatic analysis confirmed the function assigned to each protein involved in carnitine metabolism. cait was suggested to be an integral membrane protein responsible for the transport of betaines. the caia gene product was shown to be a crotonobetainyl-coa reduct ...200515731894
susceptibility testing of urinary isolates of escherichia coli to mecillinam using nccls methodology.criteria for susceptibility testing of mecillinam against 533 isolates of escherichia coli and a further 309 enterobacteriaceae, according to nccls methodology, were determined. correlation of mic to inhibition zones was good for all species. for urinary isolates of e. coli, the following agar dilution breakpoints and corresponding interpretive zone diameters seem appropriate: < or = 8 mg/l/> or = 15 mm for susceptible; 16 mg/l/12-14 mm for intermediate susceptible and > or = 32 mg/l/< or = 11 m ...200515737512
structure of the o-polysaccharide and serological studies of the lipopolysaccharide of proteus penneri 60 classified into a new proteus serogroup o70.an alkali-treated lipopolysaccharide of proteus penneri strain 60 was studied by chemical analyses and 1h, 13c and 31p nmr spectroscopy, and the following structure of the linear pentasaccharide-phosphate repeating unit of the o-polysaccharide was established: 6)-alpha-d-galp-(1-->3)-alpha-l-fucpnac-(1-->3)-alpha-d-glcpnac-(1-->3)-beta-d-quip4nac-(1-->6)-alpha-d-glcp-1-p-(o--> rabbit polyclonal o-antiserum against p. penneri 60 reacted with both core and o-polysaccharide moieties of the homologo ...200515708308
isolation of bacteria with antibiotic resistance from household cockroaches (periplaneta americana and blattella germanica).cockroaches may harbor and disseminate microorganisms to the environment. in this study, periplaneta americana and blattella germanica were collected from 40 households in kaohsiung city and kaohsiung county, taiwan. cockroach infestation was found in 50% of the studied households and 226 cockroaches (123 p. americana and 103 b. germanica) collected by trapping. p. americana was more often found in the kitchen (70.7%) whereas b. germanica in the storage room (51.5%) and kitchen (36.9%). there wa ...200515716054
pharmacodynamics of ibafloxacin in micro-organisms isolated from cats.the pharmacodynamic properties of ibafloxacin were investigated in micro-organisms isolated from cats. minimal inhibitory concentrations (mic) of ibafloxacin (racemate, r- and s-enantiomers) and its metabolites (7-hydroxy- and 8-hydroxy-ibafloxacin) and time-kill kinetics were determined against gram-negative and gram-positive bacteria isolated from dermal and respiratory and urinary tract infections in cats. racemic ibafloxacin has a broad spectrum of bactericidal activity against gram-negative ...200515720512
chondroitinase abc i from proteus vulgaris: cloning, recombinant expression and active site identification.galags (galactosaminoglycans) are one subset of the gag (glycosaminoglycan) family of chemically heterogeneous polysaccharides that are involved in a wide range of biological processes. these complex biomacromolecules are believed to be responsible for the inhibition of nerve regeneration following injury to the central nervous system. the enzymic degradation of gag chains in damaged nervous tissue by cabc i (chondroitinase abc i), a broad-specificity lyase that degrades galags, promotes neural ...200515691229
longitudinal trends in antimicrobial susceptibilities across long-term-care facilities: emergence of fluoroquinolone resistance.antibiotic resistance in the longterm-care facility (ltcf) setting is of increasing concern due to both the increased morbidity and mortality related to infections in this debilitated population and the potential for transfer of resistant organisms to other healthcare settings. longitudinal trends in antibiotic resistance in ltcfs have not been well described.200515693409
[distribution and drug-resistance of 3 500 gram-negative bacteria in guangzhou].to investigate the distribution and drug-resistance of the common gram-negative bacteria in guangzhou.200515698988
[etiology and antimicrobial susceptibility among uropathogens causing community-acquired lower urinary tract infections: a nationwide surveillance study].knowledge of antimicrobial susceptibility patterns is required to prescribe empirical therapy and formulate guidelines for the treatment of community-acquired urinary tract infections. this multicenter study assesses the etiology and antimicrobial susceptibility of the main community-acquired uropathogens in spain.200515701325
influence of various criteria for elimination of duplicates when calculating the prevalence and antibiotic susceptibility of microorganisms associated with urinary infections.the effect of applying various criteria for elimination of repeated isolates in the same patient on the prevalence and antibiotic susceptibility of microorganisms frequently associated with urinary infections were analyzed. when time criteria were applied, there was a decrease in the number of isolates of 13.4-27.5%, whereas when variations in antibiotic susceptibility were considered, between 4.3 and 20.3% of the isolates were eliminated. the absolute number of isolates varied considerably depe ...200515664489
evaluation of a new screen agar plate for detection and presumptive identification of enterobacteriaceae producing extended-spectrum beta-lactamases.a new agar screen plate for extended-spectrum beta-lactamase (esbl) detection was evaluated with 50 clinical isolates of esbl-producing enterobacteriaceae species: enterobacter cloacae (n = 10), escherichia coli (n = 10), klebsiella oxytoca (n = 3), klebsiella pneumoniae (n = 25), and proteus mirabilis (n = 2). fecal samples were artificially inoculated with 2 concentrations (25 and 250 colony forming units [cfu]/plate) of the test strains and then applied to the new agar screen plates. by this ...200515629229
nature of the promoter activated by c.pvuii, an unusual regulatory protein conserved among restriction-modification systems.a widely distributed family of small regulators, called c proteins, controls a subset of restriction-modification systems. the c proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of the protective methyltransferase when the genes enter a new cell. c proteins bind to conserved sequences called c boxes. in the pvuii system, the c boxes have been reported to extend ...200515629920
intracranial complications of otitis media: 15 years of experience in 33 patients.complications in the central nervous system (cns) from acute otitis media (aom) and chronic otitis media (com) are becoming fewer, although they still represent a challenge for early recognition, adequate treatment, and satisfactory results. this retrospective study analyzed clinical data and therapeutic options in 33 patients with intracranial involvement resulting from otitis media. important clinical features of intracranial complications and the sequence of the most efficient therapeutic man ...200515632907
clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle.bacteria contaminate the uterus of most dairy cattle after parturition and endometritis causes infertility. an endometritis score can be ascribed based on the vaginal mucus character and odour but it is not clear if the clinical score reflects the number of uterine bacteria or the inflammatory response. the present study tested the hypothesis that clinical evaluation of endometritis reflects the number of bacteria present in the uterus, and the acute phase protein response. swabs (n = 328) were ...200515589277
does the valve regulated release of urine from the bladder decrease encrustation and blockage of indwelling catheters by crystalline proteus mirabilis biofilms?we tested whether valve regulated, intermittent flow of urine from catheterized bladders decreases catheter encrustation.200515592093
contribution of the two conserved tryptophan residues to the catalytic and structural properties of proteus mirabilis glutathione s-transferase b1-1.pmgstb1-1 (proteus mirabilis glutathione s-transferase b1-1) has two tryptophan residues at positions 97 and 164 in each monomer. structural data for this bacterial enzyme indicated that trp97 is positioned in the helix a4, whereas trp164 is located at the bottom of the helix a6 in the xenobiotic-binding site. to elucidate the role of the two tryptophan residues they were replaced by site-directed mutagenesis. trp97 and trp164 were mutated to either phenylalanine or alanine. a double mutant was ...200515320869
brain abscess in children.to find out the clinical presentation, radiological characteristics, various underlying predisposing conditions and causative organisms of brain abscess in children in our setup.200519810297
metabolic activation of bladder procarcinogens, 2-aminofluorene, 4-aminobiphenyl, and benzidine by pseudomonas aeruginosa and other human endogenous bacteria.pseudomonas aeruginosa, an opportunistic pathogen of the human urinary tract, and other selected human endogenous bacteria were investigated for metabolic activation of the bladder procarcinogens, 2-aminofluorene (2-af), 4-aminobiphenyl (4-ab), and benzidine (bz). the cell-free extracts of pseudomonas aeruginosa, escherichia coli, enterobacter aerogenes, proteus mirabilis, proteus vulgaris, staphylococcus epidermidis, staphylococcus saprophyticus, klebsiella pneumoniae, and intestinal anaerobes, ...200616203120
evolutionarily conserved structural motifs in bacterial gst (glutathione s-transferase) are involved in protein folding and stability.the bacterium proteus mirabilis expresses a cytosolic class beta glutathione s-transferase (pmgst b1-1) that is part of a family of multifunctional detoxication enzymes. like other cytosolic gsts, pmgst b1-1 possesses two local structural motifs, an n-capping box and a hydrophobic staple motif, both of which are located between amino acids 151 and 156. the n-capping box consists of a reciprocal hydrogen bonding interaction of thr152 with asp155, whereas the hydrophobic staple motif consists of a ...200616248855
toxicological effect of indole and its azo dye derivatives on some microorganisms under aerobic conditions.azo dyes are ubiquitous commercial chemicals that present unique environmental problems. the azo dyes in particular can undergo natural anaerobic degradation to potentially carcinogenic amines. they pose a major problem for water-treatment plants downstream. one strategy to remidate polluted surface contaminants is to make use of the degradative capacity of bacteria rather than using destructive chemical reactions. therefore, pathogenic and non-pathogenic microorganisms namely; bacillus thuringi ...200616165191
antimicrobial, wound healing and antioxidant activity of plagiochasma appendiculatum lehm. et lind.plagiochasma appendiculatum (aytoniaceae) of the order marchantiales is widely used in the form of paste ethnomedicinally by gaddi tribe in kangra valley for treating skin diseases. in this context, antimicrobical potential of plagiochasma appendiculatum against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. th ...200616600543
purification of antibacterial agents from tragia involucrata--a popular tribal medicine for wound healing.tragia involucrata has been widely used in traditional systems of medicine for a variety of diseases. in the present study, in vitro antibacterial properties of nine different compounds including vinyl hexylether, shellsol, 2,4-dimethyl hexane, 2-methylnonane and 2,6-dimethyl heptane were isolated from the leaf of tragia involucrata studied against escherichia coli, proteus vulgaris and staphylococcus aureus using the disc-diffusion method at 50 microg/ml concentrations. the compound vinyl hexyl ...200616600546
antibiotic-lock therapy for long-term intravascular catheter-related bacteraemia: results of an open, non-comparative study.infection is one of the main problems associated with long-term central venous catheters. this study assesses the effectiveness of antibiotic-lock therapy (alt) for treating catheter-related bacteraemia (crb).200616597634
a sensor to detect the early stages in the development of crystalline proteus mirabilis biofilm on indwelling bladder catheters.a simple sensor has been developed to detect the early stages of urinary catheter encrustation and avoid the clinical crises induced by catheter blockage. in laboratory models of colonization by proteus mirabilis, the sensor signaled encrustation at an average time of 43 h before catheters were blocked with crystalline biofilm.200616597888
antibiotic treatment and resistance of unselected uropathogens in the elderly.a population-based study was conducted among women over the age of 70 years with complaints of uncomplicated urinary tract infections (utis). the positive predictive value of the nitrite test alone or in combination with the leukocyte esterase test ranged between 83% and 99%. the nitrofurantoin prescription rate decreased whereas fluoroquinolone and amoxicillin/clavulanic acid prescriptions increased with increasing age. the aetiology of infection was age-dependent. escherichia coli was the most ...200616473503
functional grouping based on signatures in protein termini.the two ends of each protein are known as the amino (n-) and carboxyl (c-) termini. short signatures in a protein's termini often carry vital cellular function. no systematic research has been conducted to address the importance of short signatures (3 to 10 amino acids) in protein termini at the proteomic level. specifically, it is unknown whether such signatures are evolutionarily conserved, and if so, whether this conservation confers shared biological functions. current signature detection me ...200616475191
global assessment of the antimicrobial activity of polymyxin b against 54 731 clinical isolates of gram-negative bacilli: report from the sentry antimicrobial surveillance programme (2001-2004).in total, 54 731 gram-negative bacilli isolated worldwide between 2001 and 2004 from diverse sites of infection were tested for susceptibility to polymyxin b by the broth reference microdilution method, with interpretation of results according to clsi (formerly nccls) guidelines. polymyxin b showed excellent potency and spectrum against 8705 pseudomonas aeruginosa and 2621 acinetobacter spp. isolates (mic50, < or = 1 mg/l and mic90, 2 mg/l for both pathogens). polymyxin b resistance rates were s ...200616524407
[proteus syndrome with cerebral vascular malformations].proteus syndrome is a congenital hamartomatous dysplasia. this sporadic disorder involves the skeletal system, soft tissues, skin and vascular system. the most likely pathogenesis involves somatic mosaicism. main manifestations included soft-tissue and epidermal nevi, partial gigantism, hemihypertrophy, exostoses, lipomas and vascular anomalies. the most common brain abnormalities are hemimegencephaly and migrational disorders. we present a case of proteus syndrome with cerebral vascular anomali ...200616525914
[protective effect of the aerosol immunization of mice with the polycomponent vaccine immunovac vp-4 after the challenge of the animals with klebsiella pneumoniae virulent strain].used four schemes of the administration of the preparation with different time of the exposition of the animals in an aerosol chamber were tested with their subsequent intraperitoneal challenge with k. pneumoniae virulent strain k16. irrespective of the number of immunization courses, the administration of the preparation made at intervals of 1 day, or daily, did not ensure any protective effect, but only led to an insignificant increase in their survival time in comparison with nonimmunized ani ...200616532635
clinical and economic impact of bacteremia with extended- spectrum-beta-lactamase-producing enterobacteriaceae.we studied outcomes of extended-spectrum beta-lactamase (esbl) production in enterobacteriaceae bacteremia. inpatients with bacteremia caused by esbl-producing escherichia coli, klebsiella spp., or proteus spp. (cases) were compared with patients with bacteremia caused by non-esbl producers (controls). outcomes included mortality, mortality due to infection, length of stay (los), delay in appropriate therapy (dat), discharge to a chronic care facility, and hospital cost. ninety-nine cases and 99 ...200616569837
in vitro antibacterial, antifungal & cytotoxic activity of some isonicotinoylhydrazide schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes.isonicotinoylhydrazide schiff's bases formed by the reaction of substituted and unsubstituted furyl-2-carboxaldehyde and thiophene-2-carboxaldehyde with isoniazid and, their co (ii), cu (ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against mycobacterium tuberculosis, escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebac ...200616570512
antibiotic susceptibility tests directly on urine samples using uro-quick, a rapid automated system.during the period june 2003-march 2004, 12,579 urine samples were examined employing the uro-quick system. positive samples (1,948) were subsequently gram-stained and processed by standard procedures for microorganism identification and antibiotic susceptibility determination by the disk diffusion method. results of this latter test were compared with those obtained employing the new rapid uro-quick method. antibiotics were introduced in vials containing 2 ml of mueller-hinton broth, then 0.5 ml ...200616572888
modulation of crystalline proteus mirabilis biofilm development on urinary catheters.the crystalline biofilms formed by proteus mirabilis can seriously complicate the care of patients undergoing long-term bladder catheterization. the generation of alkaline urine by the bacterial urease causes calcium and magnesium phosphates to precipitate from urine and accumulate in the catheter biofilm, blocking the flow of urine from the bladder. the ph at which these salts crystallize from a urine sample, the nucleation ph (ph(n)), can be elevated by diluting the urine and by increasing its ...200616585633
clonal occurrences of multidrug-resistant gram-negative bacilli: report from the meropenem yearly susceptibility test information collection surveillance program in the united states (2004).the meropenem yearly susceptibility test information collection program is a global, longitudinal resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of bacterial pathogens to carbapenems and other broad-spectrum agents. between 1999 and 2004, a total of 10-16 us medical centers referred up to 200 nonduplicate isolates from clinical infections to a central processing laboratory. over this 6-year period, the antimicrobial activity of 12 broad-s ...200616466890
cowden disease and lhermitte-duclos disease: an update. case report and review of the literature.cowden disease is a rare autosomal-dominant phacomatosis and cancer syndrome that is associated with lhermitte-duclos disease (ldd), also called dysplastic cerebellar gangliocytoma.200616459996
cmy-16, a novel acquired ampc-type beta-lactamase of the cmy/lat lineage in multifocal monophyletic isolates of proteus mirabilis from northern italy.we report multifocal detection (four different cities in northern italy) of proteus mirabilis isolates resistant to both oxyimino- and 7-alpha-methoxy-cephalosporins and producing a novel acquired ampc-like beta-lactamase. the enzyme, named cmy-16, is a variant of the cmy/lat lineage, which differs from the closest homologues, cmy-4 and cmy-12, by a single amino acid substitution (a171s or n363s, respectively) and from cmy-2 by two substitutions (a171s and w221r). expression of the cloned bla(cm ...200616436718
surgical treatment of an unusual patient with proteus syndrome. 200616432429
mössbauer identification of a protonated ferryl species in catalase from proteus mirabilis: density functional calculations on related models.the proteus mirabilis catalase is one of the most efficient heme-containing catalase and forms a relatively stable compound ii. samples of compound ii were prepared from pmc enriched in (57)fe. for the first time, two different forms of compound ii, namely low ph compound ii (lph ii) (43%) and high ph compound ii (hph ii) (25%), have been characterized by mössbauer spectroscopy at ph 8.3. the ratio lph ii/hph ii increases irreversibly with decreasing ph. the large quadrupole splitting value of l ...200616442627
a new gastrointestinal finding in proteus syndrome: report of a case of multiple colonic hemangiomas.a patient with proteus syndrome presented with lower gastrointestinal bleeding due to multiple colonic hemangiomas, a finding which has not been described previously in this syndrome. the patient was a 20-year-old man with features fulfilling the diagnostic criteria for proteus syndrome. he fulfilled both general criteria (mosaic distribution of the lesions, progressive course and sporadic occurrence) and specific criteria (including epidermal nevus, disproportionate overgrowth of limbs and vasc ...200616445505
factors affecting crystal precipitation from urine in individuals with long-term urinary catheters colonized with urease-positive bacterial species.weekly urinalysis was conducted for 12 weeks on a group of 21 long-term catheter users with confirmed catheter encrustation and urinary tract colonization with urease-positive bacteria, in order to explore the cause of considerable variation in the severity of encrustation between sufferers. the rapidity of catheter blockage correlated significantly with the ph above which crystals precipitated from urine (the nucleation ph) but not the ph of the voided urine itself. linear regression showed the ...200616453146
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