PMID(sorted ascending)
studies on bacterial nutrition : i. growth of bacillus influenzae in hemoglobin-free media.from the data presented in the foregoing experiments it is evident that bacillus influenzae will grow in a fluid medium consisting of plain broth to which have been added small amounts of emulsions or extracts of mucoid bacilli or of bacillus proteus. the bacterial extracts may be made by simple boiling of the bacillary emulsions in broth or saline solution and centrifuging out the bacterial bodies; they may be filtered without losing their growth-inducing property. cultures of bacillus influenz ...192119868534
experimental studies on the etiology of typhus fever : i. concurrent infections during the course of experimental typhus fever in guinea pigs.the work reported in this paper relates to the bacteria which can be cultivated from the blood and spleen of guinea pigs at different stages of infection with the virus of typhus fever. the studies show that during the period of incubation and before the onset of fever no ordinary bacteria appear in the cultures, while on the 1st day of the febrile reaction different bacteria were found in 6 of 26 guinea pigs cultured; on the 2nd day, in 10 of 16; on the 3rd day, in 3 of 4; and on the 4th day in ...192119868575
blood sugar studies : i. rapid alterations in the blood sugar level of rabbits as result of intravenous injections of killed bacteria of various types.a rapid rise in the blood sugar level of rabbits was produced by intravenous injections of killed bacillus proteus, bacillus coli, and bacillus paratyphosus b, which returned to nearly the previous level in a few hours time. a less pronounced rise in blood sugar was produced by killed bacillus paratyphosus a and bacillus enteritidis.192519869033
on precipitable substances derived from bacillus typhosus and bacillus paratyphosus b.attempts to confirm certain statements that ether-soluble specific substances can be obtained from b. typhosus have lead to negative results. two serologically active protein substances and another that was non-protein have been separated from b. typhosus. the first two are not resistant to tryptic digestion or to treatment with alkaline hypochlorite solution whereas the third resists both. one of the proteins could be extracted with 75 per cent alcohol. specific precipitable substances reacting ...192819869396
a serological study of the polysaccharides of meningococcus, b. anthracis, b. proteus, b. subtilis and b. mesentericus.1. the meningococcus polysaccharide reacts with a broad precipitable carbohydrate antibody in common with those of b. anthracis, b. subtilis, b. proteus and b. mesentericus. 2. the anthrax and proteus polysaccharides are specific in the higher dilutions of serum. 3. antianthrax serum contains two different polysaccharide precipitable antibodies, one specific and the other non-specific. 4. agglutinins have no relation to the carbohydrate precipitable substance, specific or non-specific. 5. an imm ...193119869953
carbohydrates adsorbed on colloids as antigens.evidence is here given that polysaccharides can be rendered antigenic by haptogenic adsorption upon a colloid carrier. the polysaccharides studied were those of b. anthracis, the meningococcus, streptococcus viridans (bargen), b. proteus, s. morgani, b. dysenteriae, both the shiga and hiss types, and the pneumococci. with the polysaccharide of type iii pneumococci, we have been unable in 6 weeks to produce any detectable protective antibodies, but we were able to produce anticarbohydrate antibod ...193219869994
studies on typhus fever : ix. on the serum reactions of mexican and european typhus rickettsia.1. the blood of guinea pigs convalescent from old world and new world typhus infection develops agglutinating properties for the tunica and rat rickettsiae of the new world diseases and for the louse rickettsia of the old world disease. 2. the two microorganisms are closely related, though probably not identical. 3. human convalescents of both varieties of typhus develop agglutinins for both types of rickettsiae. such rickettsia-agglutinating properties are parallel with the weil-felix reaction ...193219870078
studies on typhus fever : xi. a report on the properties of the serum of a horse immunized with killed formalinized rickettsia.the experiments recorded above demonstrate that the systematic treatment of a horse with formalin- and phenol-killed rickettsiae obtained from the mexican virus by our x-ray rat technique induces the development of properties in the horse's serum which may be described as follows: 1. an original agglutinating potency for proteus x-19 (weil-felix reaction) not exceeding dilutions of 1-40 was enhanced to a potency of 1-160 and, feebly, 1-320. 2. the horse's serum, after immunization, exerts distin ...193319870138
the antigenic relationship between proteus x-19 and typhus rickettsiae : a study of the weil-felix reaction.1. the absorption of typhus sera (human or antityphus horse serum) with proteus x-19 removes only the proteus agglutinins, leaving the rickettsia agglutinins intact. 2. the absorption of typhus sera with mexican rickettsiae removes the agglutinins for both the rickettsia and proteus x-19. 3. while normal or formalinized rickettsiae are not agglutinated by anti-proteus serum, these organisms-when formalinized and heated at 75 degrees c.-become agglutinable by such serum. 4. the absorption of anti ...193319870181
the antigenic relationship between proteus x-19 and typhus rickettsia : ii. a study of the common antigenic factor.a soluble specific substance was isolated from mexican typhus rickettsia which gave, with proteus x-19 antiserum and typhus human serum, the same precipitation reactions as the polysaccharides extracted from b. proteus ox-19. the soluble specific substance extracted from rickettsia and proteus ox-19 is likely to be of a polysaccharide nature owing to the strong molisch reactions obtained with such extracts, the heat stability and the negative protein reactions (biuret). since, however, it still ...193419870282
the antigenic relationship between bacillus proteus x-19 and rickettsiae : iii. a study of the antigenic composition of the extracts of bacillus proteus x-19.two substances differing in immunological behavior as well as in certain chemical properties have been isolated from soluble extracts of b. proteus x-19. both substances appear to be polysaccharides. the first substance is precipitated from x-19 crude extracts by a relatively low percentage of alcohol and electrolytes (from one to two and a half volumes of alcohol). when purified as far as possible, it gives a negative biuret reaction, a positive molish and has a nitrogen content of 4 per cent. ...193519870415
demonstration of agglutinins for bartonella bacilliformis.1. methods of preparing a satisfactory antigen having been developed, a technique for performing an agglutination test with b. bacilliformis is made available. 2. as a result of repeated intravenous injection of living cultures of b. bacilliformis at short intervals, rabbits have been found to produce a high titre of specific agglutinins which, under the conditions obtaining in the present series of experiments, begins to decline after about one month following the last inoculation. 3. sera from ...194219871168
experimental typhus infection in the eastern cotton rat (sigmodon hispidus hispidus).1. the course of typhus infection in cotton rats has been described, and a relationship between the dose administered and the time of death has been demonstrated. 2. rather incomplete pathological studies indicated that typhus in the cotton rat is an acute infection primarily involving mesothelial tissue, and tissues of mesothelial origin. depending somewhat upon the route of infection, rickettsiae were most easily demonstrated in smears from liver, brain, and pericardial and peritoneal exudates ...194419871421
the chemical alteration of a bacterial surface, with special reference to the agglutination of b. proteus ox-19.the chemical substitution of a bacterial surface has been effected by the addition of benzene sulfonyl chloride to b. proteus ox-19. the substitution of imidazole and amino groups on the bacteria has been followed by the microelectrophoretic method. concomitant changes in the agglutinability of substituted organisms have been observed, without appreciable change in the antibody-combining capacities of the organisms. the significance of these observations has been discussed.194519871488
micrurgical studies in cell physiology : i. the action of the chlorides of na, k, ca, and mg on the protoplasm of amoeba means of micro-dissection and injection amoeba proteus was treated with the chlorides of na, k, ca, and mg alone, in combination, and with variations of ph. i. the plasmalemma. 1. nacl weakens and disrupts the surface membrane of the ameba. tearing the membrane accelerates the disruption which spreads rapidly from the site of the tear. kcl has no disruptive effect on the membrane but renders it adhesive. 2. mgcl(2) and cacl(2) have no appreciable effect on the integrity of the surface membran ...192619872206
micrurgical studies in cell physiology : ii. the action of the chlorides of lead, mercury, copper, iron, and aluminum on the protoplasm of amoeba proteus.i. plasmalemma. 1. the order of toxicity of the salts used in these experiments on the surface membrane of a cell, taking as a criterion viability of amebae immersed in solutions for 1 day, is hgcl(2), fecl(3)> alcl(3)> cucl(2)> pbcl(2)> fecl(2). using viability for 5 days as a criterion, the order of toxicity is pbcl(2)> cucl(2)> hgcl(2)> alcl(3)> fecl(3)> fecl(2). 2. the rate of toxicity is in the order fecl(3)> hgcl(2)> alcl(3)> fecl(2)> cucl(2)> pbcl(2). 3. the ability of amebae to recover f ...192619872315
intracellular oxidation-reduction studies : i. reduction potentials of amoeba dubia by micro injection of indicators.twenty-five oxidation-reduction indicators were injected in oxidized or reduced form into amoeba dubia and amoeba proteus under controlled conditions of oxygen access. (1) under anaerobiosis the ameba was able to reduce completely all the reversible oxidation-reduction indicators down to and including indigo disulfonate. (2) under anaerobiosis the ameba was unable to reoxidize six of the most easily oxidizable indicators. (3) under aerobiosis the ameba was able to reduce completely all the indic ...192819872422
measurements of the metabolism of two protozoans.1. the respiration of amoeba proteus was measured. 10 c. mm. of cells were found to use about 1.6 mm.(3) of oxygen per hour at 20 degrees c. the respiratory quotient was found to be nearly unity. 2. no anaerobic metabolism was found for amoeba. 3. the respiration of blepharisma was found to be from 3 to 7 mm.(3) oxygen per hour for 10 mm.(3) cells. the respiratory quotient was about 1. 4. blepharisma was shown to have a definite anaerobic metabolism. 80 mm.(3) cells caused the evolution of 12.5 ...192919872514
on the rate of oxygen consumption by fertilized and unfertilized eggs : v. comparisons and interpretation.1. the rate of oxygen consumption by eggs may not merely undergo no change at fertilization, as in the case of the starfish, but it decreases to about half in chaetopterus and in cumingia. 2. the absolute rate of oxygen consumption in mm.(3) o(2) per hour per 10 mm.(3) eggs differs widely in several species of unfertilized eggs. it is very low in the sea urchin, intermediary in nereis, and high in chaetopterus and cumingia. the range for these eggs is approximately 0.4 to 3.1 mm.(3) o(2) per hou ...193319872719
cyanide-sensitive bacterial respiratory systems different from the usual cytochrome-cytochrome oxidase system.aerobic respiration of streptococcus pyogenes and pneumococcus type 1 are strongly inhibited by kcn, nan(3), and na(2)s. the anaerobic glycolysis of glucose by pneumococcus is also inhibited by kcn and nan(3). streptococcus pyogenes, e. coli, pneumococcus type 1, b. subtilis, b. proteus, and staphylococcus aureus did not catalyze the oxygen uptake by p-phenylenediamine in the presence of added cytochrome c or in its absence. yeast cells, b. subtilis, and b. pyocyaneus oxidized p-phenylenediamine ...194219873323
i. the relation between attachment to the substratum and ingestion by ameba in strychnine sulfate solution and conditioned media : ii. biological assay of conditioned media.1. strychnine sulfate 0.000069 m decreased percentage attachment to the substratum by amoeba proteus in 0.0029 m nacl from 77.3 to 1.3, in 0.0029 m kcl from 40.8 to 2.5, in 0.002 m cacl(2) from 73.3 to 68.0, in 0.002 m mgcl(2) from 85.5 to 83.3. 2. frequency of ingestion of chilomonads by amoeba proteus is increased by adding strychnine sulfate to solutions of nacl, kcl, or cacl(2). frequency of ingestion is increased in nacl solution from 1.3 to 2.3, in kcl from 0.75 to 2.25, and in cacl(2) fro ...194419873390
cross-reaction between proteins of larrea divaricata cav. (jarilla) and proteins of gram-negative bacteria.larrea divaricata is an abundant plant of northwest of argentina used to treat different pathologies. we aimed to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (jpce) of jarilla. we evaluated the cross reaction between jpce and whole cell-bacterial proteins (w-cbp) of escherichia coli, pseudomonas aeruginosa, proteus vulgaris, and klebsiella pneumoniae using a mouse anti-jpce serum. protein profiles of jpce and w-cbp were analyzed. for jpce, 18 bands ...200919874237
evaluation of new chromogenic substrates for the detection of evaluate a new range of chromogenic substrates for the detection of beta-galactosidase activity in coliforms and to compare their performance in agar media and broths.201019878523
assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health.200919880150
isolation of bacteria causing secondary bacterial infection in the lesions of cutaneous leishmaniasis.cutaneous leishmaniasis (cl) is a parasitic disease characterized by single or multiple ulcerations. secondary bacterial infection is one of the complications of the disease that can increase the tissue destruction and the resulting scar.200819882011
ciprofloxacin-induced hemorrhagic vasculitis.ciprofloxacin-induced hemorrhagic vasculitis is a rare side effect. it has only been described in 10 case reports since 1989. however, recently we were confronted with two cases within 1 month. in one patient the vasculitis resolved after termination of the ciprofloxacin therapy; in the other patient the ciprofloxacin-induced hemorrhagic vasculitis was superimposed on a severe forefoot infection, leading to progressive gangrene and a below-knee amputation. ciprofloxacin is among the standard tre ...201019892516
comparison of 3 phenotypic-detection methods for identifying plasmid-mediated ampc beta-lactamase-producing escherichia coli, klebsiella pneumoniae, and proteus mirabilis strains.plasmid-mediated ampc beta-lactamases (pabls) have been detected in the strains of escherichia coli, klebsiella spp., proteus mirabilis, and salmonella spp. pabls may be difficult to detect and might interfere in the therapeutic and infection-control processes. although several pabl-detection methods based on phenotypes have been reported, the clinical and laboratory standards institute currently does not recommend a routine detection method for pabls. the aim of this study is to compare the per ...200919893354
rheumatoid arthritis, proteus, anti-ccp antibodies and karl popper.rheumatoid arthritis (ra) is a crippling joint disease affecting over 20 million people worldwide. the cause of ra is most probably linked to the triad of microbial trigger, genetic association and autoimmunity and can be explained using the philosophical method of karl popper or popperian sequences. ten "popper sequences" have been identified which point to the urinary microbe proteus mirabilis as the cause of ra: popper sequence 1 establishes that hla-dr4 lymphocytes injected into a rabbit evo ...201019895906
tensile strength of absorbable suture materials: in vitro analysis of the effects of ph and evaluate the tensile strength and suture degradation of 5 commonly used absorbable suture materials in different ph and bacteria inoculated media.200919896625
joint extraintestinal manifestations in ulcerative extraintestinal manifestation (eim) very often occurs in ulcerative colitis (uc) patients. eim modifies the natural course of uc and decreases the quality of life in these patients. the aim of this study was to analyze clinical and laboratory findings in uc patients with joint eim. 319 uc patients were examined. among them were 131 (41.1%) patients with distal uc, 102 (32.0%) suffered from left-sided uc and 86 (26.9%) had pancolitis. 95 (29.8%) uc patients had joint eim. arthritis correlated ...200919897966
effect of ciprofloxacin and n-acetylcysteine on bacterial adherence and biofilm formation on ureteral stent surfaces.the aim of this study was to evaluate the effect of ciprofloxacin (cip), n-acetylcysteine (nac) alone and in combination on biofilm production and pre-formed mature biofilms on ureteral stent surfaces. two strains each of staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebseilla pneumoniae, pseudomonas aeruginosa and proteus vulgaris, recently isolated from patients undergoing ureteral stent removal and shown to be capable of biofilm production, were used in this study. the ...200919899620
the cross-reactivity of shewanella fidelis lipopolysaccharide with anti-proteus antibodies.the serological cross-reactivity between lipopolysaccharides (lps) of s. fidelis kmm3582(t) and rabbit anti-o p. mirabilis antibodies was tested. using elisa and western blot cross-reactivity between s. fidelis lps and antisera against p. mirabilis o14, o3 lpss was found. the observed cross-reaction may suggest that anti-p. mirabilis s1959 (o3) antibodies may bind to the internal part of s. fidelis o-polysaccharides. a weak interaction between s. fidelis lps and antiserum against p. mirabilis o1 ...200919899622
modification of n-glycosylation sites allows secretion of bacterial chondroitinase abc from mammalian cells.although many eukaryotic proteins have been secreted by transfected bacterial cells, little is known about how a bacterial protein is treated as it passes through the secretory pathway when expressed in a eukaryotic cell. the eukaryotic n-glycosylation system could interfere with folding and secretion of prokaryotic proteins whose sequence has not been adapted for glycosylation in structurally appropriate locations. here we show that such interference does indeed occur for chondroitinase abc fro ...201019900493
hospital and community isolates of uropathogens at a tertiary hospital in south investigate the profile of common uropathogens isolated from urine specimens submitted to the diagnostic microbiology laboratory at a tertiary teaching hospital and assess their antimicrobial susceptibility patterns to commonly used antimicrobial agents.200919908617
infections and treatment of wounds in survivors of the 2004 tsunami in thailand.on 26 december 2004, a tsunami devastated the west coast of thailand and caused 8457 injuries and 5395 deaths. data were collected from 26 december 2004 to 31 january 2005 at four public hospitals to describe the character and treatment of wounds of 523 persons who were injured during tsunami and sought medical treatment. wounds were contaminated with mud, sand, debris and sea water and had an infection rate of 66.5% (674/1013). most wounds (45%) had poly-microbial infection with gram-negative r ...200919912391
identification and characterization of the metal ion-dependent l-alanoyl-d-glutamate peptidase encoded by bacteriophage t5.although bacteriophage t5 is known to have lytic proteins for cell wall hydrolysis and phage progeny escape, their activities are still unknown. this is the first report on the cloning, expression and biochemical characterization of a bacteriophage t5 lytic hydrolase. the endolysin-encoding lys gene of virulent coliphage t5 was cloned in escherichia coli cells, and an electrophoretically homogeneous product of this gene was obtained with a high yield (78% of total activity). the protein purified ...200919919545
chronic suppurative otitis media in cleft palate: microorganism etiology and investigate the microbial etiology of suppurative chronic otitis media (scom) in patients with complete cleft lip and palate and isolated cleft palate and to determine the sensitivity of isolated microorganisms to antibiotics by drug diffusion from impregnated discs in agar and the minimum inhibitory concentration of each drug to these microorganisms by drug dilution in agar.200919929097
[clinical aspects and epidemiology of uncomplicated cystitis in women. german results of the aresc study].uncomplicated cystitis in women is among the most frequent infections in the community setting. the german results of the international aresc study are reported concerning clinical aspects, epidemiology, and antimicrobial susceptibility of uropathogens.201019943031
comparative study on characteristics of azo dye decolorization by indigenous decolorizers.this study provides a first attempt to explore indigenous strains with excellent decolorization capability from the most biodiverse region in taiwan for dye-bearing wastewater treatment. bacterial isolates were obtained via serial selections under selection pressure of the fungicide nystatin and model textile dye(s). according to profiles of protein expression and pcr-augmented 16s rrna gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to ty ...201019944603
[intracranial suppurations of otorhinolaryngological origin in children in senegal].intracranial suppurations (icss) are poorly assessed in sub-saharan africa, especially in children.201019951836
quantitative imaging of cellular adhesion by total internal reflection holographic internal reflection (tir) holographic microscopy uses a prism in tir as a near-field imager to perform quantitative phase microscopy of cell-substrate interfaces. the presence of microscopic organisms, cell-substrate interfaces, adhesions, and tissue structures on the prism's tir face causes relative index of refraction and frustrated tir to modulate the object beam's evanescent wave phase front. we present quantitative phase images of test specimens such as amoeba proteus and cells such a ...200919956284
antimicrobial properties of the stem bark of saraca indica (caesalpiniaceae).chloroform, methanol, aqueous and ethanolic extracts of the stem bark of saraca indica were investigated for their antibacterial and antifungal activity against standard strains of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus cereus, klebsiella pneumoniae, proteus mirabilis, salmonella typhimurium and streptococcus pneumoniae and the fungi: candida albicans and cryptococcus albidus. methanolic and aqueous extract exhibited antimicrobial activity with mic ranging from ...200919961043
electrostatic binding of substituted metal phthalocyanines to enterobacterial cells: its role in photodynamic inactivation.the effect of ionic substituents in zinc and aluminum phthalocyanine molecules and of membrane surface charge on the interaction of dyes with artificial membranes and enterobacterial cells, as well as on photosensitization efficiency was studied. it has been shown that increasing the number of positively charged substituents enhances the extent of phthalocyanine binding to escherichia coli cells. this, along with the high quantum yield of singlet oxygen generation, determines efficient photodyna ...200919961410
[enteropathogens relating to diarrhea in hiv patients on antiretroviral therapy].the etiology of the diarrheic process in aids may be caused by viruses, bacteria, fungi, protozoa or helminths, as well as hiv itself. this study evaluated enteropathogens relating to diarrhea in hiv patients who were on antiretroviral therapy. the parasitological methods used were faust, hoffmann and kinyoun. isolation and culturing of fungi were carried out in accordance with the methodology recommended by the nccls m27-a standard. the yeast species were identified using the polymerase chain r ...200919967238
a fatal infection with an organism of the proteus group. 192419972606
typhus-like fevers of unknown aetiology, with special reference to the malay states.typhus exanthematicus, rocky mountain fever, and the tsutsugamushi disease have been classified in the "typhus group" by megaw, as louse-typhus, tick-typhus, and mite-typhus. he has added a fourth-class, comprising typhus-like fevers, with unknown vectors. it is the diseases of this class with which this paper is concerned.endemic typhus (brill's disease) is very closely related to typhus fever; the weil-felix reaction is positive, typhus-like vascular lesions are present, and there is cross-imm ...193019987538
international spread and persistence of tem-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an inca/c2 plasmid containing tn1696::tn1 and is5075-tn21.tem-24 remains one of the most widespread tem-type extended-spectrum beta-lactamases (esbls) among enterobacteriaceae. to analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative tem-24 producers from belgium, france, portugal, and spain (13 enterobacter aerogenes isolates, 6 escherichia coli isolates, 6 klebsiella pneumoniae isolates, 2 proteus mirabilis isolates, and 1 klebsiella oxytoca isolate, from 1998 to 2004). ...201019995930
urinary tract infections caused by multi-drug resistant proteus mirabilis: risk factors and clinical outcomes.proteus mirabilis (pm) as well as other members of the enterobacteriaceae family are a leading cause of infectious diseases in both the community and acute care settings. the prevalence of multi-drug resistant (mdr) bacterial isolates have increased in the last few years, affecting the prognosis and survival of hospitalized patients. the aim of our study was to determine the risk factors and clinical outcomes of urinary tract infections (utis) caused by mdr pm in patients hospitalized in our ins ...201019998053
bacteriological evaluation of pre-cut fruits sold in kano metropolis, kano state, hundred and fifty (150) pre-cut fruit samples comprising of pineapples (50), paw-paw (50) and watermelon (50) at the point of stand retail outlets were tested by standard bacteriological methods to determine bacterial contamination of the fruits. out of these 150 examined 136 (90.67%) were contaminated with bacteria. the bacterial distribution were; escherichia coli 69 (46.00%), staphylococcus aureus 29 (19.33%), salmonella species 13 (8.67%), proteus species 18 (12.00%), enterobacter aeroge ...200920000065
structural and serological studies of the o-polysaccharide of strains from a newly created proteus o78 serogroup prevalent in polish proteus mirabilis strains from five polish patients (five isolates from urea and two from feces) appeared to be a bacterial clone widespread in hospitals, most probably due to nosocomial infection and autoinfection. enzyme-linked immunosorbent assay and western blot showed that lipopolysaccharides from all strains studied are serologically identical to each other but distinct from proteus lipopolysaccharides studied earlier and, hence, these strains could not be classified in any of the cu ...201020002745
detecting vim-1 production in proteus mirabilis by an imipenem-dipicolinic acid double disk synergy test. 201020007383
effect of ibicella lutea on uropathogenic proteus mirabilis growth, virulence, and biofilm formation.proteus mirabilis, an important uropathogen that can cause complicated urinary tract infections (uti), has emerged as a therapeutic problem following mutations that compromise the use of antimicrobial drugs. due to the serious effects associated with uropathogenic p. mirabilis and the problems related to the use of antibiotics, it is necessary to develop alternative strategies for its control. the objective of this study was to assess the effect of ibicella lutea extract, a south american indige ...200920009277
[local control treatment of bacteria found present in an infected bed sore by means of a hydropolymer dressing with ionic silver].every chronic wound has microorganisms present on its surface and therefore, its contamination is universal and unavoidable, but the majority of these wounds are not infected. it is important, therefore, to refine the diagnosis of infection indentifying the microorganisms which have succeeded in gaining access to the submarginal tissues in order to be able to prevent unnecessary or inappropriate antibiotic treatment and to lessen possible complications. a high level of bacteria present, close to ...200920014620
nosocomial infections prevalence study in a serbian university hospital.nosocomial infections (ni) are a serious health problem resulting in an enromous burden of excess morbidity and mortaliti rates, and health care costs. the aim of this study was to assess the prevalence of ni and to identify groups of patients at special risk for ni in the university clinical center, kragujevac, serbia.200920017416
microbiological findings in prepubertal girls with vulvovaginitis.the aim of the study was to define the most common causes, symptoms and clinical features of vulvovaginitis in prepubertal girls, and to evaluate treatment success depending on the causative agent involved. the study included 115 girls aged 2-8 (mean 4.8) years, presenting with vulvovaginitis to the outpatient clinic for pediatric and adolescent gynecology, zagreb children's hospital, between september 2006 and july 2007. medical history data were obtained from parents. vaginal samples were coll ...200920021979
orbus hercynius gen. nov., sp. nov., isolated from faeces of wild boar, is most closely related to members of the orders 'enterobacteriales' and pasteurellales.a novel gammaproteobacterium, strain cn3(t), was isolated from the faeces of wild boar. strain cn3(t) was facultatively anaerobic and appeared coccoid or rod-shaped. the partial 16s rrna gene sequence determined for strain cn3(t) suggested a distant relationship with members of the orders 'enterobacteriales' and pasteurellales. the gene sequence showed highest similarity (90.3 %) with obesumbacterium proteus dsm 2777(t), a member of the family enterobacteriaceae. the closest relatives outside th ...201020023064
transfer of a phage t4 gene into enterobacteriaceae, determined at the single-cell level.the transfer range of phage genes was investigated at the single-cell level by using an in situ dna amplification technique. after absorption of phages, a phage t4 gene was maintained in the genomes of non-plaque-forming bacteria at frequencies of 10(-2) gene copies per cell. the gene transfer decreased the mutation frequencies in nonhost recipients.201020023087
in vitro antimicrobial activity of a commercial ear antiseptic containing chlorhexidine and tris-edta.minimum bactericidal concentrations (mbcs) of a commercial ear antiseptic containing chlorhexidine 0.15% and tris-edta (otodine) were determined by broth microdilution for 150 isolates representing the most common pathogens associated with canine otitis. the microorganisms were classified into three groups according to their levels of susceptibility. the most susceptible group included staphylococcus pseudintermedius, malassezia pachydermatis, streptococcus canis and corynebacterium auriscanis, ...201020030799
antimicrobial susceptibility of multidrug-resistant (mdr) and extensively drug-resistant (xdr) enterobacteriaceae isolates to fosfomycin.the advancing antimicrobial drug resistance among enterobacteriaceae renders the evaluation of potential novel therapeutic options necessary. we sought to evaluate the in vitro antimicrobial activity of fosfomycin against multidrug-resistant (mdr) enterobacteriaceae isolates. antimicrobial susceptibility to fosfomycin and 12 additional antibiotics of mdr enterobacteriaceae isolates collected between november 2007 and april 2009 at the university hospital of heraklion, crete, greece, was examined ...201020034765
the influence of omentectomy on bacterial clearance: an experimental study.the influence of an omentectomy on peritoneal defense mechanisms and its clinical consequences have not been fully elucidated. in the present study, we aimed to investigate the influence of omentectomy on bacterial growth in blood and tissue specimen cultures in rats with experimental peritonitis.200920037870
proteus mirabilis sp. intestinal microflora grow in a dextran sulfate sodium-rich environment.the pathogenic mechanisms responsible for inflammatory bowel disease, especially ulcerative colitis (uc), are poorly understood. as an animal model, the oral administration of dextran sulfate sodium (dss) induces colitis, which exhibits several clinical and histological features similar to uc. however, the pathogenic factors responsible for dss-induced colitis and above all, the intestinal microflora in this colitis remain unclear. therefore, we investigated the relationships between dss and the ...201020043128
bladder hemangiomas and proteus syndrome: a rare clinical association.a case of bladder hemangiomas in association with proteus syndrome (ps) is described. ps is a rare hamartomatous disorder and comprises an association of verrucous epidermal nevi, infantile hemangiomas, lipoma-like subcutaneous hamartomas and asymmetrical overgrowth of almost any part of the body. to our knowledge, this is the second case reported of hematuria associated with bladder hemangiomas in ps.201020044313
antimicrobial resistance among clinical isolates from the chinese meropenem surveillance study (cmss), 2003-2008.the chinese meropenem surveillance study (cmss) programme was initiated in 2003 with the aim of monitoring the antimicrobial activity of broad-spectrum agents against nosocomial gram-negative bacilli in china. from 2003 to 2008, a total of 3892 isolates were collected from 10 teaching hospitals. the minimum inhibitory concentrations (mics) of 11 antimicrobial agents were determined by the agar dilution method. during the study period, a marked decrease in the susceptibility of acinetobacter spp. ...201020047820
[antimicrobial treatment of nosocomial intra-abdominal infections--new treatment options with tygecycline].nosocomial, intra-abdominal infections are extremely serious conditions, considering possibilities for their early diagnosis, as well as for their effective therapy. multiresistant bacteria (enterobacteriacae producing extended-spectrum beta-lactamases - esbl escherichia coli, klebsiella species, vancomycin-resistant enterococci [vre], and methicillin-resistant staphylococcus aureus [mrsa]) are frequently isolated as pathogens of these infections. tygecycline is among the novel wide- spectrum an ...200920052927
intra/extracellular biosynthesis of silver nanoparticles by an autochthonous strain of proteus mirabilis isolated from photographic waste.there is an enormous interest in developing green synthesis procedures for production of nanoparticles by using biomimetic approaches. in our research focus has been given to the development of an efficient and eco-friendly viable process for the synthesis of nanoscale silver particles using proteus mirabilis ptcc 1710, a bacterial strain that was isolated during a screening program from photographic waste. a significant result of this study is our observation that silver nanoparticles could be ...200920055006
discorhabdins from the korean marine sponge sceptrella sp.two new pyrroloiminoquinone alkaloids of the discorhabdin class, along with 12 compounds including one previously described synthetic derivative of the same and related skeletal classes, were isolated from the sponge sceptrella sp., collected from gageodo, korea. the structures of these new compounds, designated as (-)-3-dihydrodiscorhabdin d (11) and (-)-discorhabdin z (12), were determined by combined spectroscopic analyses. compound 12 possesses an unusual hemiaminal group among the discorhab ...201020055495
necrotizing otitis externa: 19 cases' report.necrotizing otitis externa is an uncommon but severe infectious disease of the external auditory canal. patients at risk are those immunodepressed or having diabetes. the causal germ is often pseudomonas aeruginosa. over a period of 10 years (1997-2006), we treated 19 patients: 94.7% had diabetes (insulin dependent in 6 cases). the causal germ was p. aeruginosa in 59% of cases. the pretherapeutic work-up included a computed tomography and a scintigraphy practiced in order to confirm diagnosis an ...201020058154
severe renal failure in acute bacterial pyelonephritis: do not forget corticosteroids.acute renal failure (arf) is a rare complication of acute pyelonephritis in adult immunocompetent patients. recovery of renal function usually occurs if antibiotics are promptly initiated. however, long-term consequences of renal scarring due to acute pyelonephritis are probably underestimated, and some patients present with prolonged renal failure despite adequate antibiotic therapy. we report two cases of severe arf complicating bacterial pyelonephritis successfully treated with corticosteroid ...201020061705
human pathogens abundant in the bacterial metagenome of cigarettes.many studies have evaluated chemical, heavy metal, and other abiotic substances present in cigarettes and their roles in the development of lung cancer and other diseases, yet no studies have comprehensively evaluated bacterial diversity of cigarettes and the possible impacts of these microbes on respiratory illnesses in smokers and exposed nonsmokers.201020064769
antimicrobial resistance patterns of staphylococcus aureus and proteus spp. isolated from otitis media at bahir dar regional laboratory, north west ethiopia.otitis media is a major health problem of children in low income countries like ethiopia. thus, this study was aimed at determining the predominant bacterial isolates from discharging ear and their antimicrobial susceptibility patterns.200920067141
in vitro antimicrobial activity of four ficus carica latex fractions against resistant human pathogens (antimicrobial activity of ficus carica latex).methanolic, hexanoïc, chloroformic and ethyl acetate extracts of ficus carica latex were investigated for their in vitro antimicrobial proprieties against five bacteria species and seven strains of fungi. the green fruit latex was collected from chott mariam souse, middle east coast of tunisia. the antimicrobial activity of the extracts was evaluated and based respectively on the inhibition zone using the disc-diffusion assay, minimal inhibition concentration (mic) for bacterial testing and the ...201020067867
antibacterial effect of nsaids on clinical isolates of urinary tract infection and diabetic foot infection.non steroidal anti-inflammatory drugs (nsaids) are a group of chemically dissimilar agents that have primary effect of inhibition of prostaglandin's synthesis. aspirin (acetyl salicylic acid) is used as an analgesic, anti pyretic, anti-inflammatory agent and also have an anticoagulant effect. tylenol (acetaminophen) is used as pain reliever. the objective of this study was to determine the effect of aspirin and tylenol against clinical isolates of urinary tract infection (uti) and diabetic foot ...201020067876
pathogen incidence and antibiotic resistance patterns of catheter-associated urinary tract infection in children.the aim of this study was to characterize the pathogens and their antibiotic susceptibilities in children with catheter-associated urinary tract infection (cauti) in order to optimize empirical antibiotic therapy and prophylaxis. from 2001 to 2006, 895 children with an indwelling catheter from 3 hospitals in china were included in this study, of whom 335 (37.4%) had cauti. antimicrobial susceptibility testing of 450 bacterial isolates was performed using the clsi broth and kirby-bauer agar dilut ...200920071290
autoimmune diseases: solution of the environmental, immunological and genetic components with principles for immunotherapy and transplantation.autoimmune diseases have environmental and genetic components. these are the microbial trigger, the immunity system component and the genetic component. here we describe these components and how they interact. known microbial triggers are streptococcus pyogenes for rheumatic carditis, proteus mirabilis for rheumatoid arthritis and klebsiella pneumoniae for ankylosing spondylitis. the immunity system component has been clarified by realisation that no autoimmune disease is caused by loss of suppr ...201020083235
[investigation of antibacterial activity of sertralin].sertralin is a psychotropic drug which acts by inhibiting the selective serotonin re-uptake in the synaptic area. previous studies have shown that some antidepressant agents have antibacterial activity. the aim of this study was to investigate the in vitro antibacterial activity of sertralin. a total of 224 bacterial strains isolated from clinical specimens together with standard control strains were included to the study. the antibacterial activity of sertralin was determined by microdilution m ...200920084919
antibacterial and antioxidant activities of the essential oil of artemisia echegarayi hieron. (asteraceae).artemisia echegarayi hieron. (asteraceae) is commonly known in argentina as "ajenjo". many studies report high efficacy of essential oils against food-borne pathogenic bacteria. the antimicrobial activity and minimal inhibitory concentration of a. echegarayi essential oil were evaluated against seven bacterial species of significant importance in food hygiene, by using the disc diffusion assay and the micro-well dilution method, respectively. volatile components of the extract were analyzed by g ...200920085186
[etiological factors of community acquired urinary tract infections in hospitalized patients].etiological factors of community-acquired urinary tract infections (uti) are specific for age, gender, season, complication of uti and type of uti. their prevalence and susceptibility to antimicrobial agents shows geographic and time variability.200920088156
validation of the cdc biofilm reactor as a dynamic model for assessment of encrustation formation on urological device materials.contemporary medical science is reliant upon the rational selection and utilization of devices, and therefore, an increasing need has developed for in vitro systems aimed at replicating the conditions to which urological devices will be subjected to during their use in vivo. we report the development and validation of a novel continuous flow encrustation model based on the commercially available cdc biofilm reactor. proteus mirabilis-induced encrustation formation on test biomaterial sections un ...201020091909
resistance to ciprofloxacin by enhancement of antioxidant defenses in biofilm and planktonic proteus mirabilis.antibiotic resistance and antioxidant defense were induced by ciprofloxacin in planktonic proteus mirabilis and compared with the natural antibiotic resistance of biofilm. resistant variants (1x and 1y) were obtained from cultures of the sensitive wild type "wt" strain 1 in the presence of the antibiotic. planktonic strain 1 exhibited oxidative stress with increases in the reactive oxygen species (ros) and consumption of no in the presence of ciprofloxacin, whereas 1x and 1y suffered non-signifi ...201020097163
antibacterial activity of artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria.the six organic solvent extracts of artemisia nilagirica were screened for the potential antimicrobial activity against phytopathogens and clinically important standard reference bacterial strains.201020109237
resistances of antibiotics and heavy metals in enterobacteriaceae spp. isolated from gills and intestines of achanthobrama marmid (heckel,1843) from sir dam lake turkey.a total of 94 bacteria, associated with wild achanthobrama marmid (heckel, 1843) in sir dam lake of turkey identified. subsequently selected isolates were characterized and identified to the genus level the 94 members of enterobacteriaceae were isolated in the gills and intestines, and among the isolates, e. coli were represented at a rate of 55%, shigella spp. at a rate of 21%, salmonella spp. at a rate of 9%, citrobacter spp. at a rate of 9%, klebsiella spp. at a rate of 3% and proteus spp., a ...200920112859
parental preference or child well-being: an ethical ethical dilemma that is not uncommon to encounter when caring for children occurs when parental preference does not appear to be in the child's best interest. challenges facing the health care team are further amplified when the family's cultural background does not match that of the team. a case study will be used to illustrate the challenges of a pediatric palliative care ethical dilemma further complicated by cultural diversity. review of the child's medical condition, patient/parent prefe ...201020117678
extended-spectrum beta-lactamase-producing escherichia coli in patients with travellers' diarrhoea.the identification of patients carrying extended-spectrum beta-lactamase (esbl)-producing bacteria is important, since these patients are at risk of receiving inappropriate empirical therapy if they become infected. the purpose of this study was to investigate the occurrence of esbl-producing bacteria in patients with travellers' diarrhoea. patients with travellers' diarrhoea (n = 242) having delivered stool samples for the diagnosis of salmonella, shigella, yersinia or campylobacter, were also ...201020121649
proteus mirabilis pmri, an rppa-regulated gene necessary for polymyxin b resistance, biofilm formation, and urothelial cell invasion.proteus mirabilis is naturally resistant to polymyxin b (pb). to investigate the underlying mechanisms, tn5 mutagenesis was performed, and a mutant exhibiting increased pb susceptibility was isolated. the mutant was found to have tn5 inserted into the ppmri (proteus pmri) gene, a gene which may encode a udp-glucuronic acid decarboxylase. in other bacteria, pmri belongs to the seven-gene pmrf operon, which is involved in lipopolysaccharide (lps) modification. while the ppmri knockout mutant had a ...201020123999
use of the cefepime-clavulanate esbl etest for detection of extended-spectrum beta-lactamases in ampc co-producing bacteria.extended-spectrum beta-lactamases (esbls) may not always be detected in routine susceptibility tests. this study reports the performance of the cefepime-clavulanate esbl etest for the detection of esbls in enterobacteriaceae, including those producing ampc enzyme.200920130375
antibacterial and antifungal properties of acetonic extract of feijoa sellowiana fruits and its effect on helicobacter pylori growth.feijoa sellowiana berg. fruit, widely used for human consumption, is well appreciated for its good nutritional characteristics and for its pleasant flavor and aroma. in a prior study we showed that the acetonic extract of f. sellowiana fruit exerts a potent antibacterial activity against some gram-positive and gram-negative bacterial strains. to confirm and deepen the study on antimicrobial activities of the fruit, in this article we analyze the same extract for its effectiveness against differe ...201020136454
microbial evaluation and deterioration of paints and paint-products.the microbial quality of materials and final products of a reputable paint industry in lagos area were analysed. the bacterial contaminants isolated in the paint-products included bacillus brevis, b. polymyxa, b. laterosporus, lactobacillus gasseri, l. brevis, escherichia coli and proteus mirabilis. the fungal contaminants detected in the paints were mainly aspergillus niger, a. flavus and penicillium citrinum. the microbial populations in the raw materials ranged from 1.0 x 10(6) - 9.5 x 10(6) ...200920143715
extension of an infectious abdominal aneurysm into the l3 and l4 vertebrae: report of a difficult case. 201020149710
differential characterization of biogenic amine-producing bacteria involved in food poisoning using maldi-tof mass fingerprinting.histamine poisoning is caused by the consumption of fish and other foods that harbor bacteria possessing histidine decarboxylase activity. with the aim of preventing histamine formation, highly specific mass spectral fingerprints were obtained from the 16 major biogenic amine-producing enteric and marine bacteria by means of maldi-tof ms analysis. all bacterial strains analyzed exhibited specific spectral fingerprints that enabled its unambiguous differentiation. this technique also identified p ...201020151397
assessment upon azo dye decolorization and bioelectricity generation by proteus hauseri.this study explored dye decolorization and bioelectricity generation of indigenous proteus hauseri zmd44 for dye-bearing wastewater treatment. chemical structures of azo dyes apparently affected the performance of dye biodecolorization. additions of diazo dye c.i. reactive blue 160 (rbu160) stimulated simultaneous dye decolorization and bioelectricity generation of zmd44 in single chamber microbial fuel cells (mfcs). however, high-level additions of rbu160 repressed capabilities of power product ...201020156682
characterization of udp-glucose dehydrogenase and udp-glucose pyrophosphorylase mutants of proteus mirabilis: defectiveness in polymyxin b resistance, swarming, and virulence.proteus mirabilis is known to be highly resistant to the action of polymyxin b (pb). however, the mechanism underlying pb resistance is not clear. in this study, we used tn5 transposon mutagenesis to identify genes that may affect pb resistance in p. mirabilis. two genes, ugd and galu, which may encode udp-glucose dehydrogenase (ugd) and udp-glucose pyrophosphorylase (galu), respectively, were identified. knockout mutants of ugd and galu were found to be extremely sensitive to pb, presumably bec ...201020160049
notes on the microbiology of cholesteatoma: clinical findings and treatment.anomalous proliferation of the cholesteatoma epithelium is caused by extrinsic factors such as toxins or bacterial antigens combined with lytic enzymes, lymphokines and cytokines released from the inflammatory infiltrate. this could explain the close relationship between the aggressiveness of cholesteatoma and repeated bacterial superinfection, therefore it is very important to know the bacteria involved in order to control the regrowth of skin following surgery, reduce the aggressive potential ...200920161877
antimicrobial, wound healing and antioxidant activities of anthocephalus cadamba.anthocephalus cadamba (roxb.) miq. syn a. chinensis (lamk) a. rich (rubiaceae) is ethnomedicinally widely used in the form of paste by tribe in western ghats for treating skin diseases. in this context, antimicrobial potential of a. cadamba against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing. the alchoholic and aqueou ...200720161916
antimicrobial activity of essential oils of ocimum gratissimum l. from different populations of kenya.hydro-distilled volatile oils from the leaves of ocimum gratissimum l. (lamiaceae) of 13 populations of different silvicultural zones were evaluated for antimicrobial activity against gram positive (staphylococcus aereus, bacillus spp.) and gram negative (escherichia coli, pseudomonas aeruginosa, samonella typhi, klebisiella pneumoniae, proteus mirabilis) bacteria and a pathogenic fungus, candida albicans. all the essential oils are active to the tested microbiles with different strength. the hi ...200820161936
evaluation of phytochemical and antibacterial potential of helicteres isora l. fruits against enteric bacterial pathogens.antibacterial activities of aqueous, acetone, ethanol and methanol extracts of fruits of helicteres isora (mororphali) were studied. the fruit aqueous extracts of h. isora showed prominent antibacterial activities against e. coli, staphylococcus epidermidis, salmonella typhimurium and proteus vulgaris; moderate activity against enterobacter aerogenes, staphylococcus aureus, salmonella typhi and least activity against pseudomonas aeruginosa. the aqueous extract showed maximal, the ethanol and met ...200820161950
evaluation of antimicrobial activity of the stem bark of cylicodiscus gabunensis (mimosaceae).ethyl acetate (ea) extract of the stem bark of cylicodiscus gabunensis (cg) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: escherichia coli, klebsiella pneumoniae, shigella dysenteriae, shigella flexneri, morganella morganii, proteus vulgaris, proteus mirabilis, salmonella typhi, citrobacter freundii, enterobacter cloacae, enterobacter agglomerans, staphylococcus aureus, streptococcus feacalis, pseudomonas aeruginosa ...200620162076
strategy to control catheter encrustation with citrated drinks: a randomized crossover study.nucleation ph is the ph at which ca and mg come out of urine to form crystals. if the safety margin between voiding ph and nucleation ph could be increased, it would increase the possibility of an alternative to controlling the activity of urease producing bacteria as a strategy to control catheter encrustation.201020171661
bladder vascular anomalies. 201020171931
synthesis, antibacterial and antifungal activities of some carbazole derivatives.a series of n-substituted carbazole derivatives were synthesized and evaluated for antibacterial and antifungal activities against staphylococcus aureus, methicillin-resistant staphylococcus aureus (mrsa), bacillus subtilis, escherichia coli, pseudomonas aeruginosa, bacillus proteus, candida albicans and aspergillus fumigatus by two fold serial dilution technique. some of the synthesized compounds displayed comparable or even better antibacterial and antifungal activities than reference drugs fl ...201020176480
ultrastructure of previtellogene oocytes in the neotenic cave salamander proteus anguinus anguinus (amphibia, urodela, proteidae).oogenesis in the neotenic, cave dwelling salamander proteus anguinus anguinus has not been studied yet, and this study provides a detailed description of the early growth of the oocytes. early previtellogene oocytes ranging from 100 to 600 µm in diameter were examined by light and transmission electron microscopy. the oocytes were divided into two stages based on size, color, and histology. stage i oocytes can be identified by their transparent cytoplasm and a homogenous juxtanuclear mass, compo ...201020177720
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