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the use of chromagar orientation as a primary isolation medium with presumptive identification for the routine screening of urine specimens.the aim of the study was to compare the use of a novel differential culture medium chromagar, for both primary isolation and presumptive identificaton, with the method currently used in our laboratory for screening mid-stream-urine samples (msu). routine methods (rm) included blotting paper imprinting of all specimens and additional quantitative culture on cysteine lactose electrolyte-deficient agar (cled) for selected samples together with microbact 12e for further identification. the chromagar ...199910519322
antimicrobial activity of elaeocarpus sphaericus.antibacterial activity of petroleum ether (pe), benzene (be), chloroform (ce), acetone (ae) and ethanol (ee) extracts of dried elaeocarpus sphaericus fruit was investigated against 28 gram-positive and gram-negative bacteria using the disc diffusion and plate dilution methods. the ae fraction showed marked antimicrobial activity against ten organisms. be was active against salmonella typhimurium and morganella morganii, and ee against plesiomonas shigelloides, shigella flexnerii and sh. sonneii. ...199910441793
molecular characterization of the genera proteus, morganella, and providencia by ribotyping.the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ...199910449462
in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group.we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ...199910467641
susceptibility to levofloxacin of clinical isolates of bacteria from intensive care and haematology/oncology patients in switzerland: a multicentre study.the objective of this study was to examine the susceptibility of clinical isolates to levofloxacin, a fluoroquinolone with extended activity against gram-positive bacteria, and other antibiotics in 12 swiss clinical microbiology laboratories using the nccls disc diffusion technique. isolates were prospectively collected from intensive care units (icus (59%), oncology wards (7%) and other units with haematology/oncology patients (34%) from june 1995 to march 1996. the levofloxacin breakpoints use ...199910404338
prevalence of proteus species in urinary tract infections in a regional hospital in trinidad.proteus bacteria are a well-known cause of urinary tract infections (utis). the prevalence of utis is high among catheterized patients and those undergoing manipulation of the urinary tract. this study assessed the prevalence of utis due to proteus species, the predisposing factors, complications and extent of antimicrobial resistance at a regional teaching hospital.199910418178
a novel selective nucleoside phosphorylating enzyme from morganella morganii.a selective nucleoside phosphorylating enzyme was purified to homogeneity from morganella morganii ncimb10466 crude extract. the enzyme appeared to consist of six subunits identical in molecular mass (m(r) 25,000). it phosphorylated various nucleosides at the 5'-position to produce nucleoside-5'-monophosphates using pyrophosphate as the phosphate source. energy-rich compounds, such as carbamylphosphate and acetylphosphate, were also very effective phosphate donors. the enzyme also exhibited phos ...199916232546
resolution of high-molecular-weight components in lipopolysaccharides of escherichia coli, morganella morganii, citrobacter freundii and citrobacter diversus strains with sodium dodecyl sulfate polyacrylamide gels.the use of 0.5% sodium dodecyl sulfate in polyacrylamide separation gels allowed the resolution in several bands of high-molecular-mass components in smooth lipopolysaccharide of bacterial outer membrane from escherichia coli, morganella morganii, citrobacter freundii and citrobacter diversus. with or without 0.1% sds, however, such a result was not possible.200010576704
application of the bigelow (z-value) model and histamine detection to determine the time and temperature required to eliminate morganella morganii from seafood.in new zealand, the product most frequently implicated in cases of scombroid or histamine poisoning is the hot-smoked fish, kahawai (arripis trutta). a properly controlled heating step in the production of hot-smoked seafood could eliminate bacteria able to convert the amino acid histidine to histamine. in this study, we determined the core temperatures and times required during hot smoking of kahawai to eliminate histamine-forming bacteria and to ensure a final product that will not produce his ...200010678437
histamine and biogenic amine production by morganella morganii isolated from temperature-abused albacore.histamine-producing bacteria were isolated from albacore stored at 0, 25, 30, and 37 degrees c. they were screened using niven's differential medium, and their histamine production was confirmed by high-pressure liquid chromatography analysis. the optimum temperature for growth of histamine-producing bacteria was 25 degrees c. the bacterium producing the highest level of histamine was isolated from fish abused at 25 degrees c. it was identified as morganella morganii by morphological, cultural, ...200010678431
influence of intestinal flora on the development of fibrosis and cirrhosis in a rat model.the gut flora play a significant role in the disposition of many foreign substances, as well as producing nutrients and toxins that may be absorbed and reach the liver. this study examines the influence of antibiotic-induced alterations in gut flora on the development of hepatic fibrosis in a rat model.200011129226
[two cases of purulent pericarditis as complication of myocardial infarction]. 200010723156
palliative percutaneous transhepatic gallbladder drainage of gallbladder empyema before laparoscopic cholecystectomy.this study is conducted to evaluate the feasibility of percutaneous transhepatic gallbladder drainage prior to laparoscopic cholecystectomy for the treatment of gallbladder empyema. we also determine the sonographic findings, causative organism, clinical signs and symptoms, laboratory data, associated underlying medical disorders and the complications related to both cholecystostomy and laparoscopic cholecystectomy.200011020851
type i nitroreductases in soil enterobacteria reduce tnt (2,4,6,-trinitrotoluene) and rdx (hexahydro-1,3,5-trinitro-1,3,5-triazine).many enteric bacteria express a type i oxygen-insensitive nitroreductase, which reduces nitro groups on many different nitroaromatic compounds under aerobic conditions. enzymatic reduction of nitramines was also documented in enteric bacteria under anaerobic conditions. this study indicates that nitramine reduction in enteric bacteria is carried out by the type i, or oxygen-insensitive nitroreductase, rather than a type ii enzyme. the enteric bacterium morganella morganii strain b2 with document ...200010749541
quantum mechanical study of the intermediates formed following the reaction of the histidine decarboxylase's substrate and inhibitors with coenzyme.histidine decarboxylase catalyses the decarboxylation of l-histidine to histamine using pyridoxal-5'-phosphate (plp) as coenzyme. the pm3 quantum mechanical conformation method of analysis and heat of formation calculation were carried out for intermediates which are probably formed during the interaction of histidine (substrate), (s)-alpha-methylhistidine, (s)-alpha-hydrazinohistidine, (s)-alpha-fluoromethylhistidine and (s)-alpha-difluoromethylhistidine (inhibitors) with plp-dependent histidin ...200010785554
a kinetic study on the interaction between tazobactam (a penicillanic acid sulphone derivative) and active-site serine beta-lactamases.the interaction between tazobactam and several chromosome- and plasmid-encoded (tem, shv, pse types) class a and c beta-lactamases was studied by spectrophotometry. tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner beta-lactam. a detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum beta-lactamases; the presence of a turn-over of acyl-enzyme ...200010850951
phosphorylation of nucleosides by the mutated acid phosphatase from morganella morganii.a novel nucleoside phosphorylation process using the food additive pyrophosphate as the phosphate source was investigated. the morganella morganii gene encoding a selective nucleoside pyrophosphate phosphotransferase was cloned. it was identical to the m. morganii phoc acid phosphatase gene. sequential in vitro random mutagenesis was performed on the gene by error-prone pcr to construct a mutant library. the mutant library was introduced into escherichia coli, and the transformants were screened ...200010877772
homology-based molecular modelling of plp-dependent histidine decarboxylase from mmorganella morganii.the 3-d structural information is a prerequisite for a rational ligand design. in the absence of experimental data, model building on the basis of a known 3-d structure of a homologous protein is at present the only reliable method to obtain structural information. a homology model building study of the pyridoxal 5'-phosphate (plp)-dependent histidine decarboxylase from morganella morganii (hdc-mm) has been carried out based on the crystal structure of the aspartate aminotransferase from escheri ...200010906409
isolation of bacteria from toxic dinoflagellate alexandrium minutum and their effects on algae toxicity.attempts were made to isolate the bacteria from toxic dinoflagellate alexandrium minutum t1 and to study the effect of these bacteria on the growth and toxicity of a. minutum t1. it was found that intracellular bacterial species including pasteurella haemolytica, pseudomonas vesicularis, and sphingomonas sp., and extracellular bacterial species including pasteurolla pneumotropica, morganella wisconsensis, flavobacterium oryzihabitans, pseudomonas pseudomallei, and sphingomonas sp. all of them we ...200011126517
decreased permeability and enhanced proton-dependent active efflux in the development of resistance to quinolones in morganella morganii.five quinolone-resistant strains were developed from a clinical morganella morganii isolate (m1 strain) which was susceptible to nalidixic acid and fluoroquinolones. all five in vitro selected mutants showed diminished norfloxacin accumulation and two of them also decreased the expression of the ago in the outer membrane lipopolysaccharide with respect to their parent strain and to the m. morganii nctc-235 type strain. likewise, the m1 strain did not express a 37-38 kda protein and accumulated l ...200010720807
evaluation of the sirscan automated zone reader in a clinical microbiology laboratory.we compared readings of kirby-bauer plates by the sirscan, an automated image analyzer that measures zone diameters, to those of experienced clinical microbiologists measuring zones with a hand-held caliper interfaced to a computer and with a ruler. to read plates of escherichia coli, morganella morganii, and pseudomonas aeruginosa containing 12 antibiotic disks the sirscan took 11 s; technologists took 28 s by caliper and 39 s by ruler. reading times of four different technologists ranged from ...200010747172
in vitro antibacterial spectrum of a new broad-spectrum 8-methoxy fluoroquinolone, gatifloxacin.the in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin mic90s, < or =1 mg/l, except 4 mg/l against methicillin-resistant staphylococcus aureus and enterococcus faecium). gatifloxacin was two-fold less potent than ciprofloxacin, and the same as or two-fold more potent than ofloxacin against ent ...200010747819
fragmentation of 23s rrna in strains of proteus and providencia results from intervening sequences in the rrn (rrna) genes.intervening sequences (ivss) were originally identified in the rrl genes for 23s rrna (rrl genes, for large ribosomal subunit, part of rrn operon encoding rrna) of salmonella enterica serovars typhimurium lt2 and arizonae. these sequences are transcribed but later removed during rnase iii processing of the rrna, resulting in fragmentation of the 23s species; ivss are uncommon, but have been reported in at least 10 bacterial genera. through pcr amplification of ivs-containing regions of the rrl g ...200010648538
a novel integron in salmonella enterica serovar enteritidis, carrying the bla(dha-1) gene and its regulator gene ampr, originated from morganella morganii.the genetic organization of the gene coding for dha-1 and the corresponding ampr gene was determined by pcr mapping. these genes have been mobilized from the morganella morganii chromosome and inserted into a complex suli-type integron, similar to in6 and in7. however, they are not themselves mobile cassettes. this integron probably includes a specific site for recombination allowing the mobilization of diverse resistance genes, as observed for bla(cmy-1) and bla(mox-1).200010602756
cost-effective and rapid presumptive identification of gram-negative bacilli in routine urine, pus, and stool cultures: evaluation of the use of chromagar orientation medium in conjunction with simple biochemical tests.the algorithm for a new identification system was designed on the basis of colony color and morphology on chromagar orientation medium in conjunction with simple biochemical tests such as indole (ind), lysine decarboxylase (ldc), and ornithine decarboxylase (odc) utilization tests with gram-negative bacilli isolated from urine samples as well as pus, stool, and other clinical specimens by the following colony characteristics, biochemical reactions, and serological results: pinkish to red, ind po ...200011101600
beta-lactam susceptibility patterns and investigation of cephalosporin hydrolysing beta-lactamases of gram-negative extraintestinal clinical isolates.of more than 3500 isolates of enterobacteriaceae, 48-69% were resistant to aminopenicillins and 11-45% to amoxycillin+clavulanic acid. resistance to second and third generation cephalosporins was present in 11-17 and 3-8% of escherichia coli, 47-56 and 15-52% of klebsiella-enterobacter, 36-57 and 16-27% of proteus, providencia and morganella isolates. pseudomonas aeruginosa strains varied in their resistance to antipseudomonal beta-lactams. isoelectric points, inhibitor profiles and substrate pr ...200011118847
tem-72, a new extended-spectrum beta-lactamase detected in proteus mirabilis and morganella morganii in italy.a new natural tem-2 derivative, named tem-72, was identified in a proteus mirabilis strain and in a morganella morganii strain isolated in italy in 1999. compared to tem-1, tem-72 contains the following amino acid substitutions: q39k, m182t, g238s, and e240k. kinetic analysis showed that tem-72 exhibits an extended-spectrum activity, including activity against oxyimino-cephalosporins and aztreonam. expression of bla(tem-72) in escherichia coli was capable of decreasing the host susceptibility to ...200010952610
classification, identification, and clinical significance of proteus, providencia, and morganella.this review presents the current taxonomy of the genera proteus, providencia, and morganella, along with the current methods for the identification of each species within the three genera, incorporating both conventional biochemical and commercial methods. while all of these organisms are ubiquitous in the environment, individual case reports and nosocomial outbreak reports that demonstrate their ability to cause major infectious disease problems are presented. lastly, anticipated antimicrobial ...200011023955
classification of proteus vulgaris biogroup 3 with recognition of proteus hauseri sp. nov., nom. rev. and unnamed proteus genomospecies 4, 5 and 6.strains traditionally identified as proteus vulgaris formed three biogroups. biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as proteus penneri. biogroup 2, characterized by positive reactions for indole, salicin and aesculin, was shown by dna hybridization (hydroxyapatite method) to be a genetic species separate from biogroup 1 and from biogroup 3 which is positive for indole production and negative for salicin an ...200011034498
in vitro activity of gemifloxacin against a broad range of recent clinical isolates from the usa.the antibacterial potencies of gemifloxacin (sb-265805) and 13 comparator compounds were determined by broth microdilution against a panel of 645 gram-positive and 995 gram-negative organisms collected from various usa sites. time-kill studies were performed and postantibiotic effect (pae) was determined for several organisms using trovafloxacin and ciprofloxacin as comparator compounds. based on mic(90)s, gemifloxacin was the most potent compound tested against gram-positive isolates: streptoco ...200010824027
[the structure and antibiotic sensitivity of the causative agents of community-acquired infectious diseases of bacterial origin in children].four hundred and forty pediatric patients at the age of 7 days to 15 years with various infections admitted to the hospital within a month were examined. the biological material was inoculated to blood agar on the first days of the patient admittance to the hospital and after the growth the organisms were isolated and identified. antibiotic susceptibility of the isolates was assayed with the disk diffusion method. 479 strains in all were tested. the most frequent cases requiring hospitalization ...200010768061
the hybrid-cluster protein ('prismane protein') from escherichia coli. characterization of the hybrid-cluster protein, redox properties of the [2fe-2s] and [4fe-2s-2o] clusters and identification of an associated nadh oxidoreductase containing fad and [2fe-2s].hybrid-cluster proteins ('prismane proteins') have previously been isolated and characterized from strictly anaerobic sulfate-reducing bacteria. these proteins contain two types of fe/s clusters unique in biological systems: a [4fe-4s] cubane cluster with spin-admixed s = 3/2 ground-state paramagnetism and a novel type of hybrid [4fe-2s-2o] cluster, which can attain four redox states. genomic sequencing reveals that genes encoding putative hybrid-cluster proteins are present in a range of bacter ...200010651802
natural antibiotic resistance of bacteria isolated from larvae of the oil fly, helaeomyia petrolei.helaeomyia petrolei (oil fly) larvae inhabit the asphalt seeps of rancho la brea in los angeles, calif. the culturable microbial gut contents of larvae collected from the viscous oil were recently examined, and the majority (9 of 14) of the strains were identified as providencia spp. subsequently, 12 of the bacterial strains isolated were tested for their resistance or sensitivity to 23 commonly used antibiotics. all nine strains classified as providencia rettgeri exhibited dramatic resistance t ...200011055901
deep neck infections in children.from december 1989 through 1998, a total of 68 children with deep neck infection were enrolled into this study. the mean age was 5.9 years (range, 1 month-15.8 years). infections in the retropharyngeal space (36.7%) were most common, followed by parapharyngeal space (30.8%), peritonsillar space (20.6%), and submandibular space (11.9%). fever, neck pain, and swelling were the most frequent symptoms. the most common pathogens were viridans streptococci (41%, 16/39) and staphylococcus aureus (26%, ...200111825010
primary low-grade malt lymphoma of the gallbladder.we report a case of primary low-grade b-cell lymphoma of the mucosa-associated lymphoid tissue (low-grade malt lymphoma) in the gallbladder. a 58-year-old woman suspected of gallbladder carcinoma underwent laparoscopic cholecystectomy. microscopic examination of the gallbladder demonstrated lymphoid cell infiltration forming lymphoid follicles with hyperplastic secondary follicles. the surrounding monocytoid b cells and centrocyte-like cells selectively infiltrated the crypt epithelium forming l ...200111844071
antimicrobial resistance of bacterial organisms isolated from rats.to determine if antimicrobial resistance occurs in various bacterial species isolated from rats.200112199446
[bacterial causes of meningitis in newborns].bacterial meningitis jeopardizes the life of affected newborns and in survived children often leaves permanent sequels. the sooner the diagnosis is established and the therapy is started, the more a prosperous outcome can be expected. when the indication is established, lumbar punction should be performed. on the basis of the results of cytological and biochemical analyses of the cerebrospinal fluid (csf) that point to bacterial meningitis, the initial therapy should begin even before the result ...200115637989
source and identification of histamine-producing bacteria from fresh and temperature-abused albacore.histamine-producing bacteria were isolated from fresh and temperature-abused albacore using two different isolation procedures. typically, the bacterial isolates on niven's or modified niven's medium produced negligible or low levels of histamine (<300 ppm) in histamine enumeration broth. the most frequently found species using this approach was hafnia alvei. by prescreening on selective media (eosin methylene blue [emb] agar for enteric bacteria; deman rogosa sharpe agar for lactic acid bacteri ...200111456189
necrotizing fasciitis in a newborn infant: a case report.we report the case of a one-day-old newborn infant, female, birth weight 1900 g, gestational age 36 weeks presenting with necrotizing fasciitis caused by e. coli and morganella morganii. the newborn was allowed to fall into the toilet bowl during a domestic delivery. the initial lesion was observed at 24 hours of life on the left leg at the site of the venipuncture for the administration of hypertonic glucose solution. despite early treatment, a rapid progression occurred resulting in a fatal ou ...200111460206
chemical composition and cytotoxic and antimicrobial activity of calycotome villosa (poiret) link leaves.the chemical composition of the essential oil and methanol extract of calycotome villosa (poiret) link leaves collected in sardinia (italy) has been studied by analytical and spectroscopic methods. falcarinol and some alcohols, terpenes, furan derivatives, and paraffins have been isolated from the essential oil. thirteen alkaloids and falcarinol have been identified in the chloroform fraction of the basic methanol extract. six flavonoids and four anthraquinones have been isolated in the chlorofo ...200111482772
plasmid-mediated and inducible cephalosporinase dha-2 from klebsiella pneumoniae.a klebsiella pneumoniae strain resistant to cefoxitin and oxyimino-cephalosporins was cultured from a child hospitalized in paris, france, in 1992. this isolate harboured a beta-lactamase gene located on an approximately 200 kb non-self-transferable plasmid. the beta-lactamase identified, dha-2, shared 99% amino acid identity with the ampc enzyme of morganella morganii. dha-2 was a point-mutant derivative of dha-1 identified previously in a salmonella enteritidis isolate. dha-2 expression was in ...200111157909
antimicrobial properties and mode of action of the pyrrothine holomycin.holomycin, a member of the pyrrothine class of antibiotics, displayed broad-spectrum antibacterial activity, inhibiting a variety of gram-positive and gram-negative bacteria, with the exception of enterobacter cloacae, morganella morganii, and pseudomonas aeruginosa. the antibiotic lacked activity against the eukaryotic microorganisms saccharomyces cerevisiae and candida kefyr. holomycin exhibited a bacteriostatic response against escherichia coli that was associated with rapid inhibition of rna ...200111158751
immunohistopathologic demonstration of pleuropneumonia associated with morganella morganii in a piglet.serofibrinous pleuropneumonia in a piglet was examined microbiologically and immunohistopathologically. large numbers of morganella morganii were isolated from the pneumonic lesion, but no other pathogens were identified. a large amount of m. morganii antigen was demonstrated, and its distribution was closely associated with the histologic lesion. this finding suggests that pleuropneumonia in piglets might be caused by m. morganii.200111355667
[dental sepsis as cause of fever of unknown origin]. 200111387852
multiple myeloma associated with extramedullary plasmacytoma causing nerve root compression: a case report.multiple myeloma associated with extramedullary plasmacytoma at initial presentation is rare. we describe a 45-year-old female patient with an initial presentation of low back pain and right side l5, s1 radiculopathy. there was no evidence of vertebral involvement but an epidural tumor was found later during neurosurgical intervention. the final diagnosis was immunoglobulin g, kappa multiple myeloma complicated with spinal root compression by an extramedullary plasmacytoma. no osteolytic lesion ...200111393129
antibacterial effect of protamine in combination with edta and refrigeration.the antimicrobial effect of protamine (clupeine) on a range of gram-positive and gram-negative foodborne pathogens and spoilage bacteria, was evaluated using an agar dilution assay and a broth dilution assay with alamar blue as growth indicator. protamine was tested alone at concentrations from 0 to 10,000 microg/ml, and in combination with edta (0.9 mm). assays were performed at 5 degrees c, 10 degrees c, 18 degrees c and 30 degrees c to test the effect of temperature. minimum inhibitory concen ...200111428574
growth and histamine formation of morganella morganii in determining the safety and quality of inoculated and uninoculated bluefish (pomatomus saltatrix).the objective of this study was to determine the effect of normal microflora and morganella morganii on histamine formation and olfactory acceptability in raw bluefish under controlled storage conditions. fillets inoculated with and without m. morganii were stored at 5, 10, and 15 degrees c for 7 days. microbial isolates from surface swabs were identified and screened for histidine decarboxylase activity. olfactory acceptance was performed by an informal sensory panel. histamine levels were quan ...200111770632
human v gamma 2v delta 2 t cells produce ifn-gamma and tnf-alpha with an on/off/on cycling pattern in response to live bacterial products.whereas cytokine production in alphabeta t cells is rapidly regulated by exposure to peptide ag, the mechanisms regulating cytokine production by gammadelta t cells are unknown. in this study, we demonstrate that human vgamma2vdelta2 t cells produce ifn-gamma and tnf-alpha as early as 2 h after ag exposure, and that they produce these cytokines in a dose- and time- dependent manner in response to stimulation with a live bacterial product, iso-butylamine (iba), but not to dead bacteria or lps. ga ...200111714780
antibiotic treatment to prevent urinary tract infections after urodynamic evaluation.the aim of the study was to determine the efficacy of cotrimoxazole administration after urodynamic testing to prevent urinary tract infections. in a single-blind prospective randomized study 94 women who attended for urodynamic evaluation were included. after multichannel urodynamic testing, including two catheterizations, the women received a single dose of cotrimoxazole or placebo. a clean-catch urine specimen was tested for infection after 1 week. seventy women returned a urine specimen afte ...200111569654
clinical picture: bacterial translocation in neutropenic sepsis. 200111741622
urinary tract pathogens in complicated infection and in elderly individuals. 200111171003
investigation on the nutritive value and microbiological quality of wild quail carcasses.quail meats have many advantages and superiority one the other species of poultry. this study was planned to throw plenty of light on gross chemical composition, lipid fractions, fatty acids composition, amino acids composition, of thigh and breast of male and female wild quail meat as well as the microbiological quality. the mean values of moisture, protein, fat, ash and energy contents ranged from 60.1 to 69.2%, 55.0 to 68.8%, 28.8 to 42.1%, 2.40 to 3.63% and 696 to 1000 kj, respectively. seve ...200111253642
in vitro and in vivo antibacterial activity and pharmacokinetics of sc-002 and its derivative, sc-004: new oral cephalosporins.sc-002 is a novel oral cephalosporin possessing a unique thiadiazolylethenyl moiety at the 3 position. in the present study, it was the most active against gram-positive bacteria among oral cephalosporins such as cefdinir (cfdn), cefpodoxime, cefditoren and cefaclor (ccl). it was equal to or 16 times more active than cfdn against standard and clinical strains. in particular, against clinical isolates of morganella morganii and haemophilus influenzae, sc-002 was 8-64 times more active than cfdn. ...200111306784
identification and functional characterization of arylamine n-acetyltransferases in eubacteria: evidence for highly selective acetylation of 5-aminosalicylic acid.arylamine n-acetyltransferase activity has been described in various bacterial species. bacterial n-acetyltransferases, including those from bacteria of the gut flora, may be involved in the metabolism of xenobiotics, thereby exerting physiopathological effects. we characterized these enzymes further by steady-state kinetics, time-dependent inhibition, and dna hybridization in 40 species, mostly from the human intestinal microflora. we report for the first time n-acetyltransferase activity in 11 ...200111344150
ceftibuten stability to active-site serine and metallo-beta-lactamases.ceftibuten is an oral third-generation cephalosporin active against a wide range of bacteria and shows an improved stability to hydrolysis by several beta-lactamases because of the carboxyethilidine moiety at position 7 of the ss-acyl side chain. the kinetic interactions between ceftibuten and active-site serine and metallo-ss-lactamases were investigated. the activity of several tem-derived extended spectrum beta-lactamases (esbetals) against ceftibuten, cefotaxime and ceftazidime was compared ...200111137648
[etiological factors of urinary tract infections in children].etiological factors of urinary tract infection in children have been studied. the escherichia coli, klebsiella sp., enterococcus sp. and enterobacter sp. were the most often isolated bacteria from infant and baby urine. in this age group of patients uromycoses were also affirmed. from the urine of older children (1-18 years old) mainly escherichia coli, enterococcus sp., proteus sp. and morganella morganii have been raised. uropathogens' occurrence was analysed by a statistical method in the rel ...200111852813
cefepime, piperacillin-tazobactam, and the inoculum effect in tests with extended-spectrum beta-lactamase-producing enterobacteriaceae.there is little information about the clinical effectiveness of cefepime and piperacillin-tazobactam in the treatment of infections caused by extended-spectrum beta-lactamase (esbl)-producing pathogens. some inferences have been drawn from laboratory studies, which have usually involved only one or a few strains of esbl-producing klebsiella pneumoniae or escherichia coli that produced only a limited range of esbls. such studies are indirect, sometimes conflicting, indicators of efficacy. to exte ...200111709338
comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study.the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ...200111448564
bacterial keratitis: a prospective clinical and microbiological study.to define the clinical and microbiological profile of bacterial keratitis at the jules gonin eye hospital and to test the in vitro bacterial resistance.200111423460
fatal septicemia and meningitis due to morganella morganii in a patient with hodgkin's disease.a unique case of spontaneous morganella morganii meningitis in a patient with stage iv hodgkin's disease, following hematogenous spread from the urinary tract, is described. late initiation of appropriate antibiotic treatment was probably responsible for the fatal outcome. this case illustrates the pathogenic potential of m. morganii in immunocompromised hosts.200111515771
comparative antimicrobial spectrum and activity of bms284756 (t-3811, a desfluoroquinolone) tested against 656 enterobacteriaceae, including preliminary in vitro susceptibility test comparisons and development.bms284756 (t-3811), a novel des-f(6)-quinolone, was evaluated using isolates of enterobacteriaceae from the sentry antimicrobial surveillance program tested by etest (ab biodisk, solna, sweden), reference broth microdilution and disk diffusion (5-microg) methods. ciprofloxacin, levofloxacin, gemifloxacin and gatifloxacin were also tested by broth microdilution as comparator antimicrobial agents within the same drug class. the 656 isolate collection included species from the genera citrobacter, e ...200111516937
early onset mixed morganella and klebsiella sepsis in a neonate.a premature baby girl was delivered vaginally to a mother who had an otherwise normal pregnancy, and spontaneous premature onset of labour. she had early onset neonatal sepsis with pneumonia. the baby's blood culture as well as the amniotic membrane culture grew morganella and klebsiella. she recovered on appropriate antibiotics. this is only the second reported case of early onset neonatal sepsis due to morganella. the literature is reviewed.200111519292
intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae.intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ...200111544246
gram-negative bacterial toe web infection: a survey of 123 cases from the district of cagliari, italy.foot intertrigo is mostly caused by dermatophytes and yeasts, less frequently by gram-positive and gram-negative bacteria. nevertheless, the importance of polymicrobial infections and especially colonizations of pseudomonas aeruginosa can cause therapy problems in relation to antibiotic resistance and the risk of potentially lethal complications.200111568743
identification of bacteria crucial to histamine accumulation in pacific mackerel during storage.bacterial growth and histamine formation in pacific mackerel during storage at 0, 4, 15, and 25 degrees c were monitored. to identify bacterial species contributing to histamine formation, several groups of bacteria were isolated by using selective media under temperatures corresponding to the various storage conditions. initially, low counts of bacteria were found in the gill, skin, and intestine of fresh fish, and only weak histamine formers were found in the gill. histamine was found in the m ...200111601705
acid phosphatase/phosphotransferases from enteric bacteria.we have investigated the enzymatic phosphorylation of nucleosides and found that morganella morganii phoc acid phosphatase exhibits regioselective pyrophosphate (pp(i))-nucleoside phosphotransferase activity. in this study, we isolated genes encoding an acid phosphatase with regioselective phosphotransferase activity (ap/ptase) from providencia stuartii, enterobacter aerogenes, escherichia blattae and klebsiella planticola, and compared the primary structures and enzymatic characteristics of the ...200116233057
haemagglutination, haemolysin production and serum resistance of proteus and related species isolated from clinical sources.a total of 148 strains of proteus and related species comprising of proteus mirabilis (116), proteus vulgaris (24), providentia rettgeri (4), providentia alcalifaciens (2), providentia stuarti (1) and morganella morganii (1), isolated from various sources, were examined for haemagglutination (ha), haemolysin production (hl) and serum resistance (sr). maximum isolates were obtained from urine (47.30%) and pus (40.54%) and they were multidrug resistant. the sensitivity to ciprofloxacin was 78.38%, ...200117664798
antibacterial effect of human v gamma 2v delta 2 t cells in vivo.v gamma 2v delta 2 cells, a class of t cells found only in primates, are reactive to nonpeptide organophosphate and alkylamine antigens secreted by bacteria and parasites. these cells make up 2-5% percent of human peripheral blood t cells but expand to make up 8-60% of peripheral blood t cells during bacterial and parasitic infections. we show here, using a chimeric severe combined immunodeficiency (scid) mouse (hu-scid) model, that human v gamma 2v delta 2 t cells mediate resistance to extracel ...200111696580
biogenic amines produced by enterobacteriaceae isolated from meat products.biogenic amines in ground meat and processed meat products are one of the indicators to determine the poor quality raw material. major histamine forming bacterium was escherichia coli (strain ec04 with 65.88 mg/100 ml in brain heart infusion medium), followed by the microorganisms morganella morganii (strain mm4 with 8.40 mg/100 ml and strain mm7 with 8.28 mg/100 ml) and proteus mirabilis (strain pm02 with 8.76 mg/100 ml), respectively. the highest putrescine production level was found in citrob ...200122062111
morganelliasis pneumonia in a captive jaguar.suppurative bronchopneumonia was discovered in a 6-yr-old male jaguar (panthera onca onca) that died after a 1 wk history of anorexia, depression, and respiratory difficulty. morganella morganii was isolated as a pure culture from the lung, spleen, and heart blood. this is the first record of m. morganii induced pneumonia in a jaguar.200211838216
ceftriaxone activity against gram-positive and gram-negative pathogens isolated in us clinical microbiology laboratories from 1996 to 2000: results from the surveillance network (tsn) database-usa.ceftriaxone was introduced into clinical practice in the usa in 1985 and was the first extended-spectrum (third-generation) cephalosporin approved for once-daily treatment of patients with gram-positive or gram-negative infections. review of ceftriaxone activity is important given its continued use since the mid-1980s and reports of emerging resistance among all antimicrobial agent classes. we reviewed the activity of ceftriaxone and relevant comparative agents against five gram-positive and 11 ...200212007850
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, monobactams, and carbapenems. changes in czop susceptibility for the bacteria were also evaluated with the bacterial resistance ratio calculated with the breakpoint mic. twenty-five species (3,362 strains) of gram-negative bacteria were isolated from the clinica ...200211977920
men 10700, a new penem antibiotic: in vitro antibacterial activity on clinical isolates.men 10700 is a new broad-spectrum penem, currently in preclinical development. in the present study, the activity of men 10700 was compared to that of imipenem, meropenem, cefotaxime, ampicillin/sulbactam, amikacin and ciprofloxacin against 619 gram-positive and gram-negative bacterial strains, and to that of imipenem, meropenem, cefotaxime, ceftriaxone, ceftazidime, cefepime and ampicillin/sulbactam against 38 strains of ciprofloxacin-resistant escherichia coli, and against 19 extended-spectrum ...200211901251
evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies.phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ...200211931166
[second multicenter study of the in vitro susceptibility to piperacillin-tazobactam].a study was conducted in 1999 on the activity of piperacillin-tazobactam on 1349 strains collected in a spanish multicenter study involving 10 hospitals. a total of 897 gram-negative bacteria of the following genuses were collected: escherichia coli (169), klebsiella spp. (112), pseudomonas spp. (122), enterobacter spp. (92), serratia spp. (44), citrobacter spp. (37), proteus spp. (114), morganella spp. (49), acinetobacter spp. (47), stenotrophomonas spp. (47), haemophilus spp. (45), moraxella s ...200212582446
identification and characterization of pvua, a gene encoding the ferric vibrioferrin receptor protein in vibrio parahaemolyticus.we previously reported that vibrio parahaemolyticus expresses two outer membrane proteins of 78 and 83 kda concomitant with production of siderophore vibrioferrin in response to iron starvation stress and that these proteins are the ferric vibrioferrin receptor and heme receptor, respectively (s. yamamoto, t. akiyama, n. okujo, s. matsuura, and s. shinoda, microbiol. immunol. 39:759-766, 1995; s. yamamoto, y. hara, k. tomochika, and s. shinoda, fems microbiol. lett. 128:195-200, 1995). in this s ...200211807053
enhancement of nucleoside phosphorylation activity in an acid phosphatase.escherichia blattae non-specific acid phosphatase (eb-nsap) possesses a pyrophosphate-nucleoside phosphotransferase activity, which is c-5'-position selective. current mutational and structural data were used to generate a mutant eb-nsap for a potential industrial application as an effective and economical protein catalyst in synthesizing nucleotides from nucleosides. first, gly74 and ile153 were replaced by asp and thr, respectively, since the corresponding replacements in the homologous enzyme ...200212200535
[yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria].the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ...200212071094
comparative evaluation of two commercial chromogenic media for detection and presumptive identification of urinary tract pathogens.the performance of two commercial chromogenic media for the isolation and presumptive identification of urinary tract pathogens, the cps id2 (biomérieux, france) and the chromagar orientation (bbl becton dickinson, usa), was evaluated and compared with that of cystine-lactose-electrolyte-deficient agar and tryptic soy agar with 5% sheep blood. the detection, determination of bacterial counts, and presumptive identification of bacteria causing urinary tract infections were evaluated in 3,000 urin ...200212072939
emerging fluoroquinolone-resistance for common clinically important gram-negative bacteria in taiwan.the in vitro antimicrobial activity of the three most commonly used fluoroquinolones (norfloxacin, ofloxacin and ciprofloxacin) was measured for 2235 isolates of seven common pathogenic gram-negative bacteria. collected from a major teaching hospital in taiwan during the years 1985-86, 1989-90, and 1996-97, the samples were evaluated using the agar dilution method. the overall susceptibility to fluoroquinolones has decreased rapidly after the wide use of these antimicrobial agents. isolates of e ...200212088622
brain abscess caused by aerobic gram-negative bacilli: clinical features and therapeutic outcomes.thirty-three patients (24 males and nine females) with brain abscesses resulting from infection by aerobic gram-negative bacilli were identified at kaohsiung chang gung memorial hospital over a period of 14 years. of these, 23 cases developed spontaneously, with the remaining ten postneurosurgery. the organisms most frequently involved were klebsiella (k.) pneumoniae, pseudomonas aeruginosa, escherichia coli and proteus species and included some rare pathogens, such as salmonella and enterobacte ...200212445926
[pathogenicity islands in opportunistic enterobacteria].the presence of fragments of genomes hlya, hlyb, papah, papc, sfag, sfaa and kps mt, associated with the pathogenicity islands of escherichia coli, in clinical strains of other genera of the family enterobacteriaceae, has been experimentally evaluated with the use of pcr. the presence of dna fragments specific to the known genes of the pathogenicity clusters of e. coli in representatives of the genera citrobacter, enterobacter, hafnia, klebsiella, morganella, proteus, providencia, serratia and y ...200212506618
[antibacterial activity of cefpodoxime against clinical isolates in 2000 and 2001].as the post-marketing surveillance of cefpodoxime proxetil (banan), mics of cefpodoxime (cpdx, an active form of banan) against 1090 clinical isolates of 22 species from 15 medical institutions all over japan from june 2000 to march 2001 were measured using the broth microdilution method approved by the japanese society of chemotherapy and compared with those of oral cephem antibacterials, cefaclor, cefdinir, cefditoren, and cefcapene. in this study, remarkable change in the activity of cpdx was ...200212621736
morganella morganii causing solitary liver abscess complicated by pyopericardium and left pleural effusion in a nondiabetic patient.morganella morganii is a rare cause of solitary liver abscess in taiwan. the complication of pyopericardium and pleural effusion in nondiabetic patient with solitary liver abscess are also rare. we present a case of a 48-year-old nondiabetic woman who experienced with epigastric discomfort 1 month prior to admission. chills and fever developed 2 weeks before admission. physical examination on admission revealed engorgement of the jugular vein over the right neck, precordial friction rubs, and te ...200212380794
liver abscess metastasizing to prostate and lung. 200212411623
structural investigation of the o-specific polysaccharides of morganella morganii consisting of two higher sugars.the lipopolysaccharide of the bacterium morganella morganii (strain kf 1676, rk 4222) yielded two polysaccharides, ps1 and ps2, when subjected to mild acid degradation followed by gpc. the polysaccharides were studied by 1h and 13c nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, 1h,(13)c hmqc, and hmbc experiments. each polysaccharide was found to contain a disaccharide repeating unit consisting of two higher sugars, 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-l-glycero-d-galact ...200212423973
early-onset neonatal sepsis caused by vertical transmission of morganella morganii.the onset of morganella morganii infection within the first 72 h after birth has only been reported on 1 occasion. the authors describe a second case in which m. morganii was cultured from both the neonate's blood and the mother's lochia. the infant was successfully treated with cefotaxime and gentamicin.200212195880
new synthetic catecholate-type siderophores with triamine backbone.new analogues of triscatecholate siderophores based on linear or tripodal triamines with or without spacer groups or lipophilic and hydrophilic substituents were synthesized. the catecholate moieties were prepared in oh-forms, as acetylated compounds or masked as 8-methoxycarbonyloxy-2,4-dioxo-1,3-benzoxazine derivatives. some of the new compounds were active as siderophores tested by growth promotion assays using various gram-negative bacteria and mycobacteria under iron limitation and by cas-a ...200212046921
[occurrence of beta-lactamase types esbl and ampc among gram-negative rods isolated from food].the aim of the study was to isolate extended spectrum beta-laktamases (esbl) and chromosomal beta-laktamases ampc producing strains from food products. a total of 739 gram-negative bacteria were tested with double disc diffusion method using cefotaxim, ceftasidim and amoxycillin with clavulanic acid. one strain producing esbl and belonging to stenotrophomonas maltophilia was detected from the ice cream (0.14% of all strains). from different food products a total of 14 microorganisms (1.9%) havin ...200212185694
modification of niven's medium for the enumeration of histamine-forming bacteria and discussion of the parameters associated with its use.the objective of this study was to improve niven's medium (nm) for the optimized enumeration of histamine-forming bacteria (hfb). the parameters modified related to solidification of the agar at low ph values (ph 5.3 to 5.8), incubation time (24, 48, and 72 h) and temperature (30 and 37 degrees c), number of colonies developed on the plate to allow enumeration of hfb, and color differentiation. strains of hfb, morganella morganii, klebsiella pneumoniae, and hafnia alvei were examined for their a ...200211899055
[morganella morgagnii septic arthritis]. 200211940403
monoassociation of scid mice with helicobacter muridarum, but not four other enterics, provokes ibd upon receipt of t cells.recently, a number of animal models for different aspects of inflammatory bowel disease (ibd) have been developed. the aim of this study was to use one of these to determine whether particular, ostensibly innocuous, intestinal bacteria could provoke or exacerbate ibd.200211984521
antibacterial activities and characterization of novel inhibitors of lpxc.lipid a is the hydrophobic anchor of lipopolysaccharide (lps) and forms the major lipid component of the outer monolayer of the outer membrane of gram-negative bacteria. lipid a is required for bacterial growth and virulence, and inhibition of its biosynthesis is lethal to bacteria. udp-3-o-(r-3-hydroxymyristoyl)-n-acetylglucosamine deacetylase (lpxc) is a metalloenzyme that catalyzes the second step in the biosynthesis of lipid a. inhibitors of lpxc have previously been shown to have antibiotic ...200212019092
development of a sensitive and specific enzyme-linked immunosorbent assay for detecting and quantifying cmy-2 and shv beta-lactamases.polyclonal rabbit antibodies against shv-1 and cmy-2 beta-lactamases were produced and characterized, and enzyme-linked immunosorbent assays (elisas) were developed. immunoblots revealed that the anti-shv-1 antibody recognized shv-1 but did not recognize tem-1, k-1, oxa-1, or any ampc beta-lactamase tested. the anti-cmy-2 antibody detected escherichia coli cmy-2, enterobacter cloacae p99, klebsiella pneumoniae act-1, and the ampc beta-lactamases of enterobacter aerogenes, morganella morganii, an ...200212037047
[surveillance of susceptibility of clinical isolates to cefmetazole between 2000 and 2002].the antibacterial activity of cefmetazole (cmz) against clinical isolates from 15 medical institutions all over japan was evaluated yearly for two years from june 2000 to march 2002 and compared with that of other parenteral beta-lactams, cefazolin (cez), cefotiam (ctm), sulbactam/cefoperazone (sbt/cpz), and flomoxef (fmox). in the first surveillance from june 2000 to march 2001, 575 isolates of 13 species were tested, and 548 isolates of the same 13 species were tested in the second surveillanc ...200315007873
[post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria].as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, monobactams, and penicillins. changes in czop susceptibility among bacteria were also evaluated with the bacterial resistance ratio calculated from the breakpoint mic. twenty-five species (4,154 strains) of gram-negative bacteria were isolated from the clinical ...200314692381
what have we learnt from community-acquired infections in hong kong?this study was initiated throughout hong kong, to reveal the characteristics of community-acquired infections. all specimens collected by general practitioners from infected patients were followed prospectively, and those that were culture-positive were analysed. four thousand seven hundred and forty-one specimens were collected from 3977 patients by 89 doctors from july 2000 to october 2001. the most common specimens were throat swabs (33%), urine (26%) and sputa (16%). the average culture-posi ...200312654732
[morganella morganii endophthalmitis after vitrectomy: case report and review of the literature].postoperative bacterial endophthalmitis are caused in 80 % of the cases by the patient's own flora. most of the time, bacterial agents are gram-positive ((2/3) of cases) and more rarely gram-negative ((1/3) of cases). usually, pseudomonas sp, proteus sp or klebsiella sp are isolated, but very rarely morganella morganii.200312664382
characterization of clinical isolates of enterobacteriaceae from italy by the bd phoenix extended-spectrum beta-lactamase detection method.production of extended-spectrum beta-lactamases (esbls) is an important mechanism of beta-lactam resistance in enterobacteriaceae: identification of esbls based on phenotypic tests is the strategy most commonly used in clinical microbiology laboratories. the phoenix esbl test (bd diagnostic systems, sparks, md.) is a recently developed automated system for detection of esbl-producing gram-negative bacteria. an algorithm based on phenotypic responses to a panel of cephalosporins (ceftazidime plus ...200312682131
bacteraemia due to tribe proteeae: a review of 132 cases during a decade (1991-2000).to characterize the clinical features of bacteraemia due to tribe proteeae, 132 cases among 130 patients from 1991 to 2000 were analysed. the organisms included the proteus species in 63 cases (p. mirabilis in 41, p. penneri in 2 and p. vulgaris in 20), the providencia species in 8 (p. rettgeri in 3 and p. stuartii 5) and morganella morganii in 61. morganella bacteraemia occurred more frequently in the hospital (70.5%). biliary and hepatic diseases were predominant in cases with morganella bacte ...200312693558
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