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cloning and characterization of the napa acid phosphatase/phosphotransferase of morganella morganii: identification of a new family of bacterial acid-phosphatase-encoding genes.the gene encoding a minor phosphate-irrepressible acid phosphatase (named napa) of morganella morganii was cloned and sequenced, and its product characterized. napa is a secreted acid phosphatase composed of four 27 kda polypeptide subunits. the enzyme is active on several organic phosphate monoesters but not on diesters, and is also endowed with transphosphorylating activity from organic phosphoric acid esters to nucleosides and other compounds with free hydroxyl groups. its activity is inhibit ...19957894706
in vitro activity of subinhibitory concentrations of quinolones on urea-splitting bacteria: effect on urease activity and on cell surface hydrophobicity.the effect of subinhibitory concentrations of ciprofloxacin, lomefloxacin, norfloxacin, ofloxacin, and sparfloxacin on urease activity and on cell surface hydrophobicity of urea-splitting bacteria was examined. quinolones at 0.5 mics demonstrated variable effects on bacterial-urease activity. norfloxacin inhibited enzyme activity in proteus vulgaris and proteus mirabilis, while other quinolones had no effects. in morganella morganii, sparfloxacin and ciprofloxacin enhanced urease activity, parti ...19957844396
purification and properties of crystalline 3-methylaspartase from two facultative anaerobes, citrobacter sp. strain yg-0504 and morganella morganii strain yg-0601.3-methylaspartase (3-methylaspartate ammonia-lyase, ec 4.3.1.2) from two facultative anaerobes from soil, citrobacter sp. strain yg-0504 and morganella morganii strain yg-0601, were purified and crystallized from their crude extracts. both of the citrobacter and morganella enzymes appeared to be a dimer of subunits of m(r) 40,000 and 44,000, respectively. the enzymes had similar enzymological properties: optimum ph for the deamination reaction of (2s,3s)-3-methylaspartic acid, substrate specific ...19957765982
the in-vitro activity of cp 99,219, a new naphthyridone antimicrobial agent: a comparison with fluoroquinolone agents.the in-vitro activity of a new fluoroquinolone, cp 99,219 was compared with that of ciprofloxacin, du 6859, sparfloxacin and levofloxacin. cp 99,219 showed generally similar in-vitro activity to the other compounds tested against the enterobacteriaceae (mic90 of all agents < 0.5 mg/l except morganella morganii). it was found to be more active than ciprofloxacin, sparfloxacin and levofloxacin against the strains of acinetobacter calcoaceticus tested (mic90 1 mg/l). cp 99,219 was found to be four- ...19957559198
ferric rhizoferrin uptake into morganella morganii: characterization of genes involved in the uptake of a polyhydroxycarboxylate siderophore.iron uptake in morganella morganii, mediated by the fungal siderophore rhizoferrin, was studied. a mud1 insertion mutant devoid of growth on ferric rhizoferrin was complemented by a chromosomal dna fragment of m. morganii that encoded an outer membrane protein and a periplasmic protein named ruma and rumb (for rhizoferrin uptake into morganella spp.), respectively. ruma and rumb have the same transcription polarity and are probably cotranscribed from an iron-regulated promoter upstream of ruma. ...19968550472
[fluoroquinolone and aminoglycoside resistance in chromosomal cephalosporinase-overproducing gram-negative bacilli strains with inducible beta-lactamase].the increasing prevalence of stable derepressed mutants over-producers of type i chromosomal cephalosporinase in inducible enterobacteriaceae and pseudomonas aeruginosa challenges the adequacy of third generation cephalosporins in the empirical treatment of certain nosocomial infections. we sought to determine the frequency of stable over-producers of type i enzyme and their associated resistance to fluoroquinolones and aminoglycosides.19969044634
[antimicrobial activity of cefodizime against clinical isolates].in order to evaluate antimicrobial activity of cefodizime (cdzm), minimum inhibitory concentrations (mics) of cdzm and control drugs were determined against clinical isolates collected from nation-wide medical institutions and in our laboratory from september to december of 1992 and from september to december of 1995. the results are summarized as follows: 1. bacterial species with no or few strains resistant to cdzm included streptococcus pyogenes, haemophilus influenzae, citrobacter koseri, pr ...19968986558
scope of urinary pathogens isolated in the public health bacteriology laboratory, harare: antibiotic susceptibility patterns of isolates and incidence of haemolytic bacteria.to determine the scope of urinary pathogens isolated in the public health bacteriology laboratory, their antibiotic susceptibility patterns and the incidence of haemolytic bacteria in harare.19968990570
a study of nosocomial diarrhea in calcutta.nosocomial infection is a major problem in hospitalized patients, particularly those who are debilitated. these infections may manifest as diarrhea. the spectrum of infections agents causing nosocomial diarrhea in our country is not known.19968840619
a simple method to detect bacteriolytic enzymes produced by enterobacteriaceae.the production of bacteriolytic enzymes by enterobacteriaceae in various growth conditions was investigated. peptone-based media containing killed gram-negative cells facilitated detection of bacteriolytic enzyme production in the highest number of species. these belonged to the genera serratia, proteus, morganella and providencia. in contrast, escherichia coli, shigella, salmonella, klebsiella, enterobacter and citrobacter species did not produce bacteriolytic activities in any of the condition ...19968893522
prevalence of pathogenic role of morganella-proteus providencia-group of bacteria in human faeces.speculation in literature that organisms of the morganella-proteus-providencia (mpp) group can cause diarrhoea led to the present studies which investigated their occurrence and pathogenic role in human faeces. faecal specimens were collected from a total of 307 subjects, 80 of which were from diarrhoea cases and 277 from healthy controls. ninety-two species of the mpp (24/80 diarrhoea and 68/277 control) were isolated from 30% of all subjects. none of the species of the mpp group was significan ...19969110049
[putaminal abscess occurring at the site of hemorrhage: a case report].brain abscesses following stroke have been reported only rarely. we presented a case of putaminal abscess following putaminal hemorrhage. the patient was admitted to our hospital because of acute onset of left hemiplegia. the size of the hematoma was medium and the patient was conservatively treated. in about two months after the ictus, he became intermittently febrile and laboratory examinations suggested the presence of general infections including meningitis. meanwhile, ct and mri revealed cl ...19968827738
[antimicrobial activities of tosufloxacin against recently obtained clinical isolates].to evaluate antimicrobial activities of tosufloxacin (tflx), minimum inhibitory concentrations (mics) of tflx and other drugs were determined against clinical isolates that were obtained in our laboratory from february of 1993 to january of 1994 (period i), and from february of 1995 to january of 1996 (period ii). the results are summarized as follows: 1. compared to mic90's reported by others for tflx in the 1980's, those obtained by us against beta-streptococci, streptococcus pneumoniae, pepto ...19968828073
biochemical investigations of biogroups and subspecies of morganella morganii.we determined the subspecies and biogroup designations for 73 strains of morganella morganii principally recovered from routine clinical specimens. on the basis of trehalose fermentation, 90% of all strains were identified as m. morganii subsp. morganii (trehalose negative), while the remaining 10% were designated m. morganii subsp. sibonii (trehalose positive). using three tests (ornithine decarboxylase [odc] and lysine decarboxylase [ldc] activities and susceptibility to tetracycline), we dete ...19968748284
[antimicrobial activities of cefetamet against clinical isolates from urinary tract infection].in order to evaluate antimicrobial activity of cefetamet (cemt), minimum inhibitory concentrations (mics) of cemt and control drugs were determined against gram-negative rods mainly from complicated urinary tract infections examined in our laboratory from april to september of 1994. the results are summarized as follows; 1. the obtained strains were citrobacter diversus 20, citrobacter freundii 30, enterobacter aerogenes 20, enterobacter cloacae 30, serratia marcescens 30, proteus mirabilis 30, ...19969032594
a bifunctional urease enhances survival of pathogenic yersinia enterocolitica and morganella morganii at low ph.to infect a susceptible host, the gastrointestinal pathogen yersinia enterocolitica must survive passage through the acid environment of the stomach. in this study, we showed that y. enterocolitica serotype o8 survives buffered acidic conditions as low as ph 1.5 for long periods of time provided urea is available. acid tolerance required an unusual cytoplasmically located urease that was activated 780-fold by low-ph conditions. acid tolerance of helicobacter species has also been attributed to u ...19968932305
[cytolysins and homologous proteins produced by gram-negative bacteria. ii. hemolysins of aeromonas, serratia, proteus and morganella and proteins from gram-negative bacteria related to cytolysins].this article presents the genetic determination, synthesis and activation of hemolysins produced by mentioned above bacteria, as well as the possible role of these toxins in the pathogenicity. all these cytolysins are related to rtx proteins presented to the previous paper. the short information about cytotoxic proteins from other gram-negative bacteria (p. aeruginosa, k. pneumoniae, vibrionaceae, g. vaginalis) and homologous proteins from e. chrysanthemi and r. leguminosarum biovar viciae are a ...19969019745
identification and characterization of phon-sf, a gene on the large plasmid of shigella flexneri 2a encoding a nonspecific phosphatase.a gene encoding a nonspecific phosphatase, named phon-sf, was identified on the large virulence plasmid (pmysh6000) of shigella flexneri 2a ysh6000. the phosphatase activity in ysh6000 was observed under high-phosphate conditions. however, it was found that low-phosphate conditions induced a slightly higher level of activity. the nucleotide sequence of the phon-sf region cloned from pmysh6000 possessing the phon-sf gene encoded 249 amino acids with a typical signal sequence at the n terminus. th ...19968755883
incidence of histamine-forming bacteria and histamine content in scombroid fish species from retail markets in the barcelona area.incidence and diversity of histidine decarboxylating bacteria were determined in samples of tunafish, bonito and mackerel purchased at different retail markets. histamine-forming bacteria occurred in a low proportion and always accounted for less than 0.1% of the total bacterial load in the fish samples studied. similarly, histamine content in fish samples also was low ( < 25 ppm) and all of them met current histamine standards established by the european union. histamine was found in 83.3% of t ...19968652349
prevalence, antimicrobial properties and beta-lactamase production of haemolytic enterobacteria in patients with diarrhoea and urinary tract infections in legos, nigeria.to determine the prevalence, antimicrobial properties and beta-lactamase production of haemolytic enterobacteria in patients with diarrhoea and urinary tract infections in lagos, nigeria.19968771935
[in vitro antibacterial activity of a new parenteral penem, sulopenem].eighty percent minimum inhibitory concentrations (mic80) of sulopenem against clinically isolated 12 to 80 strains of each of different bacteria were as follows: methicillin-susceptible staphylococcus aureus (mssa): 0.20 micrograms/ml, methicillin-resistant s. aureus (mrsa): 50 micrograms/ml, coagulase-negative staphylococci: 3.13 micrograms/ml, streptococcus pyogenes: < or = 0.013 microgram/ml, streptococcus pneumoniae: < or = 0.013 microgram/ml, beta-streptococci: 0.05 microgram/ml, enterococc ...19968786624
[in vitro and in vivo antibacterial activities of sulopenem, a new penem antibiotic].the in vitro and in vivo antibacterial activities of sulopenem, a new penem, were evaluated in comparison with imipenem (ipm), meropenem (mepm), ceftazidime (caz) and flomoxef (fmox). sulopenem had broad and potent antibacterial spectra against gram-positive and gram-negative bacteria, including enterococcus faecalis, proteus vulgaris, morganella morganii, enterobacter spp. and citrobacter freundii. sulopenem showed concentration-dependent bactericidal activities against staphylococcus aureus, e ...19968786626
distribution of diaminopropane and acetylspermidine in enterobacteriaceae.polyamines of 97 strains (60 species) belonging to 18 genera of the family enterobacteriaceae were determined by high performance liquid chromatographic analysis. in addition to putrescine and cadavarine, diaminopropane was widely distributed in enterobacteriaceae and almost ubiquitously within enterobacter, pantoea, erwinia, leminorella, proteus, leclercia, morganella, klebsiella, hafnia, rahnella, serratia, and tatumella species and sporadically within citrobacter, escherichia, moellerella, pr ...19968742354
isolation and characterisation of dog uropathogenic proteus mirabilis strains.proteus mirabilis strains isolated from the urine of dogs with urinary tract infections, were characterised with respect to the production of haemolysin and fimbriae. in contrast to healthy dogs, p. mirabilis was also isolated in high numbers from the faeces of dogs suffering from recurrent urinary tract infections. production of fimbriae was demonstrated by electron microscopy and the presence of genes for two different types of major fimbrial subunits (mr/p-like or uca-like) was demonstrated b ...19968701578
piperacillin/tazobactam in the treatment of hospitalized patients with urinary tract infections: an open non-comparative and multicentered trial.the aim of this multicentered, prospective and open study was to determine the clinical and bacteriological efficacy and safety of piperacillin/tazobactam (4g/500 mg iv tid) in the treatment of 79 adult patients with complicated urinary tract infections (uti) requiring hospitalization. forty-seven women and 32 men (mean age 54.2 years, and range 21-91) from 4 argentinean and 6 mexican hospitals were enrolled. sixty-one clinically and bacteriologically evaluable patients were treated for a mean o ...19968708743
coordination chemistry of the carboxylate type siderophore rhizoferrin: the iron(iii) complex and its metal analogs.rhizoferrin is a member of a new class of siderophores (microbial iron transport compounds) based on carboxylate and hydroxy donor groups rather than the commonly encountered hydroxamates and catecholates. we have studied the coordination chemistry of rhizoferrin (rf), as a representative of this group, with fe(3+), rh(3+), cr(3+), al(3+), ga(3+), vo(2+), and cu(2+). the metal complexes have been studied by uv-vis, cd, nmr, and epr spectroscopies and mass spectrometry. the formation constants fo ...199611666790
belgian multicentre study on the in vitro activity of cefepime against gram-negative bacilli.the in vitro activity of cefepime has been compared with that of cefotaxime, ceftazidime, aztreonam, and piperacillin against 1826 enterobacteriaceae including 537 inducible enterobacteriaceae (enterobacter spp., serratia spp., citrobacter spp,. morganella morganii) and 572 non-fermenters, including 401 pseudomonas aeruginosa and 111 acinebacter spp. isolated from hospitalized patients in 28 belgian hospitals. overall, cefepime was found substantially more active than the third-generation cephal ...19968669160
inhibitory effect of spices on in vitro histamine production and histidine decarboxylase activity of morganella morganii and on the biogenic amine formation in mackerel stored at 30 degrees c.the inhibitory effects of clove, cinnamon, cardamom, turmeric and pepper on the histamine production and histidine decarboxylase activity of morganella morganii (a potent histamine-producing bacteria in fish) was examined at 30 degrees c using hplc. cinnamon and clove exhibited a significant (p < 0.01) inhibitory effect, whereas turmeric and cardamom had a moderate effect. these spices were applied to whole mackerel at a level of 3% and their inhibitory effect on biogenic amine formation at 30 d ...19968765991
pyomyositis caused by morganella morganii in a patient with aids. 19968838200
comparative in vitro studies on the beta-lactamase-inhibiting effect of clavulanic acid and sulbactam on ampicillin-resistant enterobacteriaceae.clavulanic acid and sulbactam alone are ineffective against pseudomonas and enterococci. staphylococci and enterobacteriaceae are inhibited by high concentrations not attainable under clinical conditions. strains of acinetobacter, especially a. lwoffii are susceptible to clavulanic acid and highly susceptible to sulbactam. the ampicillin/sulbactam combination is superior to ampicillin/clavulanic acid against citrobacter freundii, enterobacter spp. and morganella morganii. the latter combination ...199618611726
bactericidal activity of cefodizime on enterobacteriaceae in an in-vitro model simulating plasma pharmacokinetics in humans.an in-vitro dialysis model was employed to assess the feasibility of once-daily dosing of cefodizime in the treatment of infections caused by various enterobacteriaceae: escherichia coli, klebsiella pneumoniae, morganella morganii, serratia marcescens, providencia stuartii and enterobacter cloacae. this model simulated the concentrations of cefodizime detected in human blood after an intravenous (i.v.) bolus injection of 1 g or 2 g of the antibiotic. validation of the model was undertaken to con ...19979069535
high frequency of antibiotic resistance among gram-negative isolates in intensive care units at 10 swedish hospitals.objective: to investigate the resistance rates among gram-negative isolates in swedish intensive care units (icus). methods: during 1994-95, members of the swedish study group collected, on clinical indication, 502 consecutive initial isolates of gram-negative bacteria from patients admitted to icus at 10 swedish hospitals and performed minimal inhibitory concentration (mic) determinations with the etest. breakpoints were defined according to the criteria of the swedish reference group for antib ...199711864106
occurrence and detection of extended-spectrum beta-lactamases in members of the family enterobacteriaceae at a veterans medical center: seek and you may find.a total of 907 consecutive isolates of members of the family enterobacteriaceae recovered during a 20-week period were tested for production of extended-spectrum beta-lactamases (esbls) by the double-disk (dd) potentiation method. of 84 dd-positive isolates, 83 (9.2%) produced esbls based on isoelectric focusing. shv-derived esbls and several tem-derived esbls were present in nine species, including the first isolate of citrobacter koserii and morganella morganii known to harbor an shv-derived e ...19979316913
plasmid-encoded fosfomycin resistance in bacteria isolated from the urinary tract in a multicentre survey.sixty out of 219 fosfomycin-resistant bacteria selected from more than 7400 urinary pathogens in an epidemiological multicentre survey performed in italy were screened for plasmid genes fosa and fosb conferring fosfomycin resistance. only five strains, three enterobacteria and two staphylococci, carried plasmids harbouring, respectively, fosa and fosb genes. fosfomycin resistance in the other isolates was caused by an alteration of the chromosomally encoded glpt transport system. one strain, mor ...19979338493
cloning and sequencing of the gene encoding the ampc beta-lactamase of morganella morganii.the chromosomal beta-lactamase gene of a clinical isolate of morganella morganii was cloned in escherichia coli and sequenced. the beta-lactamase had a pi of 7.4 and conferred a typical ampc susceptibility pattern. the insert obtained was found to encode a protein of 379 amino acids. its deduced amino acid sequence revealed it to be a class c beta-lactamase: 39-56% identity with chromosomal ampc beta-lactamases of serratia marcescens, yersinia enterocolitica, citrobacter freundii, enterobacter c ...19979066104
antibacterial activity of bms-180680, a new catechol-containing monobactam.the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ...19979145861
[validation of tkt medium for detection of streptococcus agalactiae in bulk milk samples].a selective medium for quantitative determination of streptococcus agalactiae in bulk milk samples was tested. among the medium bases, edwards medium (ew) supplemented with 6 to 9% (v/v) of carefully washed sheep erythrocytes was proved to be best (tab. v). the production of sphingomyelinasis c (bhe, tab. ii) or d (corex, tab. i) as a supplement supporting the formation of specific hemolytic zones in the medium, was tested in four strains of staphylococcus aureus and seven strains of corynebacte ...19979182394
susceptibility pattern of uropathogenic gram negative bacilli to antimicrobial chemotherapeutic agents in a national hospital in dar es salaam.in a period of two months, 232 consecutive urinary tract pathogens were isolated from hospitalised and non-hospitalised patients. among the isolates, 200 (86.2%) were gram negative bacilli, including e. coli 109 (54.5%), klebsiella species, 44 (22.5%), enterobacter species 19 (9.5%), proteus species 18 (9%), morganella morganii 9 (4.5%) and salmonella typhimurium, one (0.5%). antimicrobial susceptibility testing to amoxycillin/clavulanic acid, nitrofurantoin, gentamicin and cefuroxime was perfor ...19979185413
in-vitro activity of trovafloxacin against sensitive and resistant aerobic bacteria using the standard microdilution broth method and etest.a comparison of mics of trovafloxacin (cp-99,219) determined by the standard microdilution broth method versus the etest was performed for multiple strains of gram-positive and gram-negative bacteria. a comparison was also made of the in-vitro activity of trovafloxacin versus ciprofloxacin and ofloxacin. the mic50 and mic90 were determined by both methods for each species tested. the etest resulted in mics one to two dilutions higher than the microdilution broth method. trovafloxacin was the mos ...19979222068
morganella morganii: a newly reported, rare cause of neonatal sepsis.this case report reviews the clinical course of an 11-day-old boy who developed late-onset neonatal sepsis secondary to a rare neonatal pathogen, morganella morganii. this gram-negative enteric bacterium, within the enterobacteriaceae family, has most commonly been a nosocomial pathogen in debilitated, postsurgical patients. like many other enterobacteriaceae, m. morganii has an inducible beta-lactamase and is resistant to multiple antibiotics. when caring for neonates with culture-proven m. mor ...19979223696
upper urinary tract stones & ureaplasma urealyticum.extensive culture of stones and of pre-operative and renal pelvic urine for isolation of bacteria and ureaplasma urealyticum were performed in 70 patients of nephrolithiasis. stones were subjected to biochemical analysis and scanning electron microscopy. micro-organisms were isolated from 33 (47%) of 70 renal stones. of the 38 species of micro-organisms isolated, 14 were urea-splitting (u. urealyticum 2; klebsiella pneumoniae 8; morganella morganii 1; acinetobacter spp 3) and 24 were nonurea spl ...19979029830
the most frequent aminoglycoside resistance mechanisms--changes with time and geographic area: a reflection of aminoglycoside usage patterns? aminoglycoside resistance study groups.the aminoglycoside resistance mechanisms revealed by two surveys in europe and other countries have been compared to those revealed in earlier studies. mechanisms have become more complex in all bacterial groups. in providencia, serratia, pseudomonas, acinetobacter, and staphylococcus species isolates, genus-specific mechanisms were very common, and it was not possible to see differences between different geographic areas. in other enterobacteriaceae, the increasing complexity of mechanisms was ...19978994779
morganella morganii postoperative endophthalmitis. 19979059257
the in-vitro activity of faropenem, a novel oral penem.the in-vitro activity of faropenem, a novel oral penem, was studied in comparison with other beta-lactam antimicrobials against 711 recent clinical isolates including gram-negative, gram-positive and anaerobic bacteria. mic data showed that faropenem was active against most members of the enterobacteriaceae (mics < or = 4 mg/l), with reduced activity against serratia spp. (mic90 = 32 mg/l). in common with its comparators, faropenem had weak activity against pseudomonas aeruginosa and stenotropho ...19979044026
evaluation of bbl chromagar orientation medium for detection and presumptive identification of urinary tract pathogens.the microbiological performance of bbl chromagar orientation medium and cps id2 agar was compared to that of columbia agar with 5% sheep blood and macconkey agar without crystal violet for the enumeration and presumptive identification of bacteria responsible for urinary tract infections. of a total of 658 clinical urine specimens, 118 specimens yielded no growth, 402 specimens yielded growth with cell counts of > or = 10(5) cfu/ml, and 138 specimens yielded growth with cell counts of < 10(5) cf ...19979350731
relapsing henoch-schönlein purpura associated with a tubo-ovarian abscess due to morganella morganii.henoch-schönlein purpura is the most common vasculitis in children. it is frequently preceded by either viral or bacterial infections of the upper respiratory tract. we present a 15-year-old female who developed recurrent episodes of henoch-schönlein purpura. eventual investigation of the patient's abdominal pain, initially attributed to vasculitis involving the gastrointestinal tract, revealed a left tubo-ovarian abscess caused by morganella morganii. salpingo-oophorectomy resulted in complete ...19979382169
3-methylaspartate ammonia-lyase as a marker enzyme of the mesaconate pathway for (s)-glutamate fermentation in enterobacteriaceae.the enzyme 3-methylaspartase (3-methylaspartate ammonia-lyase, ec 4. 3.1.2) was found in the cells of enteric bacteria, especially in the genera citrobacter and morganella, that were grown under anoxic and oxygen-limited conditions. the enzymes were purified to homogeneity from the cell-free extracts of 18 active strains and had similar enzymological properties such as action on columns, specific activity, molecular weight, subunit structure, and n-terminal amino acid sequence similarity. the pr ...19979385136
[monotherapy with meropenem in febrile granulocytopenic patients].infection remains the major cause of morbidity and mortality for cancer patients who become granulocytopenic. combinations of beta-lactams plus aminoglycosides have been the standard empiric therapy for febrile granulocytopenic patients, especially those with profound long-lasting granulocytopenia. the advent of new broad-spectrum cephalosporins and carbapenems has favoured the possibility of empiric monotherapy. meropenem is a parenteral carbapenem antibiotic stable to renal dehydropeptidase-i ...19979410073
nitrate-reducing bacteria in diversion colitis: a clue to inflammation?a pathogenic role of nitric oxide has been suggested in acute and chronic intestinal inflammation. we took the opportunity offered by studies in patients with excluded colon, which represents a model of chronic intestinal inflammation with no exogenous nitrite or nitrate supply, to evaluate the quantity and the quality of nitrate reducers in diversion colitis. thirty patients (17 men, 13 women, mean age 45 years) having an excluded colon for various reasons were sampled by rectal swabs and compa ...19979440640
changing trends in frequency and antimicrobial resistance of urinary pathogens in outpatient clinics and a hospital in southern israel, 1991-1995.in order to monitor changes in the frequency and antimicrobial resistance of urinary pathogens over several years, urinary cultures received from outpatient clinics and from a hospital during a period of one month each in 1991 and 1995 were analyzed at a clinical microbiology laboratory. in 1991 and 1995, 1366 and 1534 significant monomicrobic cultures respectively were reviewed. the frequency of escherichia coli dropped significantly in the outpatient clinics from 70.5% to 61.2% (p < 0.0001). t ...19979447906
molecular characterization of a tem-21 beta-lactamase in a clinical isolate of morganella morganii.a clinical isolate of morganella morganii, with reduced susceptibility to expanded-spectrum cephalosporins and aztreonam, was found to produce an extended-spectrum beta-lactamase with a pi of 6.4. the nucleotide sequence of the encoding gene was that of the gene encoding tem-21. this is the first molecular characterization of an extended-spectrum beta-lactamase in m. morganii.19989687421
interactions of beta-lactamases with sanfetrinem (gv 104326) compared to those with imipenem and with oral beta-lactams.sanfetrinem is a trinem beta-lactam which can be administered orally as a hexatil ester. we examined whether its beta-lactamase interactions resembled those of the available carbapenems, i.e., stable to ampc and extended-spectrum beta-lactamases but labile to class b and functional group 2f enzymes. the comparator drugs were imipenem, oral cephalosporins, and amoxicillin. mics were determined for beta-lactamase expression variants, and hydrolysis was examined directly with representative enzymes ...19989593145
imipenem resistance in aerobic gram-negative bacteria.a prospective study was undertaken to observe the emergence of resistance to imipenem, if any, among aerobic gram-negative bacteria. a total of 736 isolates were tested during 1994-95 and less than 1% of them were resistant to imipenem, whereas the next year ('95-'96) the rate increased to 11 of the 903 isolates tested. the resistant isolates during '94-'95 were all stenotrophomonas maltophilia whereas the spectrum of resistant bacterial species increased in '95-'96 to include pseudomonas aerugi ...19989603633
case of chorioamnionitis in an immunocompetent woman caused by morganella morganii.morganella morganii is an opportunistic organism that leads to serious morbidity. it is an enteric organism commonly associated with immunocompromise and chronic urinary catheterization. we present a case of a healthy gravid individual who presented with chorioamnionitis and neonatal seizures associated with m. morganii. this organism, which causes severe morbidity, is fortunately rare in obstetrics and gynecology.19989502663
bacterial acid phosphatase gene fusions useful as targets for cloning-dependent insertional inactivation.the morganella morganii phoc gene, encoding a class a acid phosphatase, was used to generate gene fusions with modified amino-terminal moieties of the escherichia coli lacz gene carrying a multiple-cloning site flanked by phage-specific promoters and recognition sites for universal sequencing primers. the corresponding hybrid proteins retained a phoc-like enzymatic activity which is easily detectable by a plate histochemical assay, rendering similar gene fusions potentially useful as targets for ...19989548775
morganella morganii early onset sepsis. 19989877376
[in vitro antimicrobial activity of cefpirome compared to other broad-spectrum beta-lactam drugs against 804 clinical isolates from 9 brazilian hospitals].to evaluate the in vitro activity of the fourth-generation cephalosporin cefpirome in comparison to that of ceftazidime, ceftriaxone, cefotaxime and imipenem in a multicenter study involving nine hospitals from six cities (four states).19989852647
microbiological examinations and in-vitro testing of different antibiotics in therapeutic endoscopy of the biliary system.prior to endoscopic therapeutic procedures, no antibiotic prophylaxis is administered routinely. because of the reported incidence of infectious complications, which may reach up to 10%, a prospective study was undertaken to investigate the effects of a prophylactic dose of cefuroxime on the incidence of bacteremia and clinical signs of infection, but no significant effects could be demonstrated. in addition to this published work, blood and bile cultures obtained in this trial were also investi ...19989865561
ecthyma gangrenosum-like eruption associated with morganella morganii infection.ecthyma gangrenosum is considered as a pathognomonic sign of pseudomonas aeruginosa sepsis. lesions similar to ecthyma gangrenosum may be caused by other organisms. we report a case of an ecthyma gangrenosum-like eruption caused by morganella morganii, a gram-negative bacillus.19989767304
[a national multicenter study of the in-vitro activity of piperacillin-tazobactam. the spanish piperacillin-tazobactam group].the action of piperacillin-tazobactam on 4,137 of the 4,364 strains collected in a spanish multicenter study involving 46 participating hospitals was studied. the samples were from the following: 41% urine, 18% exuded from wound abscesses, 13% respiratory, 9% blood, 3% peritoneal liquid, and the remainder from other various sources. the gram-negative bacteria included 2,778 strains from 13 genera: escherichia (1,289), pseudomonas (451), proteus (230), klebsiella (203), haemophilus (172), enterob ...19989795299
[physiologic-biochemical features of morganella under various conditions of cultivation].the parameters of the cultivation process and the content of protein and polysaccharide in m.morganii cells grown under the conditions of submerged cultivation in 2 nutrient media having different composition were studied. the study revealed that the highest values of specific growth rate and the maximum yield of biomass were observed during cultivation under the conditions of medium values of oxygen concentration in the medium (po2 = 20-30%). the content of protein in bacterial cells was practi ...19989825487
[comparative study of morganella and providencia bacteriophages].7 strains of m.morganii phages and 7 strains of p.rettgeri phages were isolated from lysogenic cultures and the environment. the main biological properties of these phages were studied. the phages under study formed independent taxonomic groups. these phages were found to be highly specific and capable of being used for the identification of bacteria.19989825491
bacterial isolates from california sea lions (zalophus californianus), harbor seals (phoca vitulina), and northern elephant seals (mirounga angustirostris) admitted to a rehabilitation center along the central california coast, 1994-1995.in 2 yr of bacteriologic culturing of 297 california sea lions (zalophus californianus), 154 harbor seals (phoca vitulina), and 89 northern elephant seals (mirounga angustirostris) stranded alive along the california coast, the most frequent isolates from inflammatory lesions in live animals were escherichia coli, streptococcus viridans, listeria ivanovii, beta-hemolytic streptococcus spp., and enterococcus spp. this is the first report of l. ivanovii isolation from a marine mammal. the common i ...19989732032
salmonella enteritidis: ampc plasmid-mediated inducible beta-lactamase (dha-1) with an ampr gene from morganella morganii.dha-1, a plasmid-mediated cephalosporinase from a single clinical salmonella enteritidis isolate, conferred resistance to oxyimino-cephalosporins (cefotaxime and ceftazidime) and cephamycins (cefoxitin and moxalactam), and this resistance was transferable to escherichia coli hb101. an antagonism was observed between cefoxitin and aztreonam by the diffusion method. transformation of the transconjugant e. coli strain with plasmid pnh5 carrying the ampd gene (whose product decreases the level of ex ...19989736562
[a female case of fournier's gangrene in a patient with lupus nephritis].infection is one of the common causes of death in patients with systemic lupus erythematosus (sle). it is associated with the use of immunosuppressive agents, renal failure, and increased disease activity. fournier's gangrene is a necrotizing fasciitis occurring in the genital region. it is rare, but can be crucial if surgical drainage is delayed. we report a female case of fournier's gangrene occurring in a patient with lupus nephritis and chronic renal failure. the patient was a 21-year-old fe ...19989752693
[antibiotic sensitivity to isolated bacteria in pediatric urinary tract infections].of the childhood urinary tract infections, more than 50% are caused by escherichia coli (e coli), followed by proteus mirabilis (p mirabilis), klebsiella sp, other enterobacteria, enterococci, pseudomonas aeruginosa, and staphylococci. of e coli isolates, 50 to 60% are resistant to ampicillin (ampi-r), 10% being susceptible to amoxicillin + clavulanic acid (amc). for p mirabilis, ampi-r isolates are less frequent and more often susceptible to amc. klebsiella sp is resistant to ampicillin, 75% of ...19989759315
study of rapidity and quality for the vitek susceptibility cards with 45 wells.vitek 45 wells card was evaluated. recent clinical isolates of gram-positive cocci (107) and gram-negative bacilli (179) were antimicrobial susceptibility testing by vitek system in comparison with mic-2000 system. the antimicrobial susceptibility results were available within of 8 h for gram-positive cocci without a part of mrsa. enterobac-teriaceae were available within of 8 h and pseudomonas aeruginosa were from 8 to 11 h. the antimicrobial susceptibility data had disagreement of 60% with em, ...199810036375
identification and natural antibiotic susceptibility of morganella morganii.our data should elucidate whether or not natural antibiotic susceptibility can be used as an aid for subspecies or biovar discrimination of morganella morganii (ii). furthermore, our goal was to create a database of the natural susceptibility of m. morganii (iii) and we were interested in the relative frequency of the recently described subspecific taxa (i). on the basis of trehalose fermentation (tre), ornithine decarboxylase (odc), and lysine decarboxylase (ldc) activities, we determined the b ...19989572021
important and emerging beta-lactamase-mediated resistances in hospital-based pathogens: the amp c enzymes.resistance to third-generation cephalosporins mediated by beta-lactamases is an increasing problem for clinical therapeutics. a wide range of enterobacteriaceae produce these ampc enzymes (bush-jacoby-medeiros group 1), including enterobacter spp., citrobacter freundii, morganella morganii, providencia spp., and serratia marcescens. resistance via this mechanism has been shown to be statistically correlated with the use of some third-generation cephalosporins, and the infections caused by these ...19989635237
[pharmacokinetic, bacteriological and clinical evaluation of tazobactam/piperacillin in pediatrics].pharmacokinetic, bacteriological and clinical studies were performed in pediatrics on tazobactam/piperacillin (taz/pipc), a combined drug of a new beta-lactamase inhibitor tazobactam and piperacillin at a ratio of 1:4. 1. serum levels and urinary excretions of taz, pipc and desethyl piperacillin (det-pipc), a metabolite of pipc, after one shot intravenous administration of 50 mg/kg of taz/pipc to two children (6-7 years old) were investigated. the serum taz level at 0.08 hour was 50.8-51.0 micro ...19989693967
[bactericidal activity and capacity for selection of mutants resistant to imipenmen and meropenem in strains of proteus, morganella and providencia].to compare the bactericidal activity and frequency of mutation for meropenem and imipenem against proteus mirabilis, morganella morganii and providencia rettgeri.19989763741
gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria.naturally produced membrane vesicles (mvs), isolated from 15 strains of gram-negative bacteria (citrobacter, enterobacter, escherichia, klebsiella, morganella, proteus, salmonella, and shigella strains), lysed many gram-positive (including mycobacterium) and gram-negative cultures. peptidoglycan zymograms suggested that mvs contained peptidoglycan hydrolases, and electron microscopy revealed that the murein sacculi were digested, confirming a previous modus operandi (j. l. kadurugamuwa and t. j. ...19989765585
a family of stability determinants in pathogenic bacteria.a novel segregational stability system was identified on plasmid r485, which originates from morganella morganii. the system is composed of two overlapping genes, stbd and stbe, which potentially encode proteins of 83 and 93 amino acids, respectively. homologs of the stbde genes were identified on the enterotoxigenic plasmid p307 from escherichia coli and on the chromosomes of vibrio cholerae and haemophilus influenzae biogroup aegyptius. the former two homologs also promote plasmid stability in ...19989829958
utility of serial rectal swab cultures for detection of ceftazidime- and imipenem-resistant gram-negative bacilli from patients in the intensive care unit.forty-four patients receiving intensive care were studied prospectively to assess the utility of serial rectal swab cultures and clinical correlates of resistance for pseudomonas aeruginosa, enterobacter spp., citrobacter spp., morganella morganii, and serratia marcescens strains resistant to ceftazidime or imipenem. strains of pseudomonas aeruginosa, enterobacter spp., citrobacter spp., or morganella morganii were found in 26 of 44 (59%) patients: 17 (65%) in clinical sites (11 with concomitant ...19989865988
[descriptive study of infections caused by central venous catheters with peripheral insertion].to know the infection rate for central venous catheters inserted via a peripheral vein (cvcipv), the microorganisms involved, and the incidence of complications derived from catheter infections.19989934059
unusual case of postoperative infection caused by morganella morganii.morganella morganii is a facultative gram-negative anaerobe present in the human gastrointestinal system as normal flora. clinically, the organism is important when it manifests as an opportunistic pathogenic infection elsewhere in the body. this paper reviews an unusual case in which m. morganii is the pathogen responsible for a devastating postoperative infection in a diabetic patient. currently, there are no known reported cases in the literature of m. morganii as an etiologic pathogen for a ...19989571462
studies on the formation of crystalline bacterial biofilms on urethral catheters.a model of the catheterised bladder was used to test the ability of urease-producing urinary tract pathogens to encrust urethral catheters. encrustation was assessed by determining the amounts of calcium and magnesium deposited on the catheters and visualised by scanning electron microscopy. urease-positive morganella morganii, klebsiella pneumoniae, and pseudomonas aeruginosa failed to raise the urinary ph and form crystalline biofilms. in contrast, strains of proteus mirabilis, proteus vulgari ...19989832268
fatal early onset infection in an extremely low birth weight infant due to morganella morganii.this paper reports a case of chorioamnionitis due to morganella morganii in a mother who presented with ruptured membranes at 24 weeks' gestation and was treated with dexamethasone and prophylactic ampicillin. her premature infant developed severe early onset infection due to the same organism and expired.199910685307
in vitro and in vivo activities of syn2190, a novel beta-lactamase inhibitor.syn2190, a monobactam derivative containing 1,5-dihydroxy-4-pyridone as the c-3 side chain, is a potent inhibitor of group 1 beta-lactamase. the concentrations of inhibitor needed to reduce the initial rate of hydrolysis of substrate by 50% for syn2190 against these enzymes were in the range of 0.002 to 0.01 microm. these values were 220- to 850-fold lower than those of tazobactam. syn2190 showed in vitro synergy with ceftazidime and cefpirome. this synergy was dependent on the concentration of ...199910428909
[the results of a multicenter study of the comparative activity of cefepime and other antibiotics against the causative agents of severe hospital infections (the micromax program)].a multicentre trial was performed on the activity of cefepime in comparison with ceftazidime, ceftriaxone, piperacillin/tazobactam, imipenem and ciprofloxacin against severe hospital infection pathogens in intensive care units. the isolates of escherichia coli and proteus spp. from the majority of the centres were highly susceptible to the antibiotics (90 to 100 per cent of the isolates). in some centres up to 40 per cent of the isolates produced esbl. the isolates of klebsiella spp. were charac ...199910629736
molecular recognition of synthetic siderophore analogues: a study with receptor-deficient and fhu(a-b) deletion mutants of escherichia coli.the biological activity of six synthetic siderophore analogues (two dihydroxamates, two trihydroxamates, one tetrahydroxamate and one 3-hydroxy-4(1h)pyridinone) has been studied in escherichia coli, morganella morganii 13 and proteus mirabilis 8993 strains by using growth promotion tests. various transport-deficient mutants of e. coli were used to study the route of entry into gram-negative bacteria. the results indicated that the synthetic hydroxamate compounds are transported via fhu-mediated ...199910581683
cefotaxime-hydrolysing beta lactamases in morganella morganii.the frequency of enterobacterial isolates with high resistance to expanded-spectrum beta-lactam antibiotics (mainly cefotaxime or ceftriaxone) has increased notoriously in argentina, mainly because of the spread of extended-spectrum beta-lactamases. the aim of this work was the study of extended-spectrum beta-lactamases in several morganella morganii isolates with unusually high resistance to ceftriaxone. these strains produced at least two beta-lactamases, of apparent pis of 5.4 and 8.2, molecu ...199910584905
comparative evaluation of five chromogenic media for detection, enumeration and identification of urinary tract pathogens.five chromogenic agar plates--cps id2 medium (biomérieux, france), chromagar orientation medium (becton dickinson, france), uriselect3 medium (sanofi diagnostics pasteur, france), rainbow agar uti medium (biolog, usa) and chromogenic uti medium (oxoid, germany)--for the detection, enumeration and direct identification of urinary tract pathogens were compared using 443 urine specimens at two hospital laboratories. the enumeration of microorganisms was consistent on the five media for 403 of the 4 ...199910614954
bacteremia caused by digestive system endoscopy.to evaluate bacteremias caused during endoscopic examination of the digestive tract.199910231302
prevalence of proteus species in urinary tract infections in a regional hospital in trinidad.proteus bacteria are a well-known cause of urinary tract infections (utis). the prevalence of utis is high among catheterized patients and those undergoing manipulation of the urinary tract. this study assessed the prevalence of utis due to proteus species, the predisposing factors, complications and extent of antimicrobial resistance at a regional teaching hospital.199910418178
[the use of minipigs to produce diagnostic sera for the serological identification of bacteria in the genus morganella].mini-pigs of the svetlogorsk breed were immunized with vaccine prepared from of m. morganii culture; the culture had been grown in a liquid nutrient medium, obtained from casein hydrolysate, in flasks placed in a thermostatically controlled shaker to the phases of exponential growth and cell growth deceleration. o-agglutinating sera thus obtained were specific. the highest antibody level was detected in the serum obtained as the result of the immunization of guinea pigs with the vaccine prepared ...199910356725
[verification of identification of infectious agents by culture in routine gynecologic cytological screening].the original bethesda classification system for reporting cervical/vaginal cytologic diagnoses has claimed besides oncologic evaluation also a statement on the presence of infectious agents. their diagnosis should be followed by appropriate treatment. based upon the comparison of careful bacterioscopic study in a series of 175 routine cervical smears with the results of microbiological, virological and mycological examinations the following pathogens might be-according to the authors' opinion-di ...199911038666
antimicrobial activity of elaeocarpus sphaericus.antibacterial activity of petroleum ether (pe), benzene (be), chloroform (ce), acetone (ae) and ethanol (ee) extracts of dried elaeocarpus sphaericus fruit was investigated against 28 gram-positive and gram-negative bacteria using the disc diffusion and plate dilution methods. the ae fraction showed marked antimicrobial activity against ten organisms. be was active against salmonella typhimurium and morganella morganii, and ee against plesiomonas shigelloides, shigella flexnerii and sh. sonneii. ...199910441793
phylogenetic analysis of l4-mediated autogenous control of the s10 ribosomal protein operon.we investigated the regulation of the s10 ribosomal protein (r-protein) operon among members of the gamma subdivision of the proteobacteria, which includes escherichia coli. in e. coli, this 11-gene operon is autogenously controlled by r-protein l4. this regulation requires specific determinants within the untranslated leader of the mrna. secondary structure analysis of the s10 leaders of five enterobacteria (salmonella typhimurium, citrobacter freundii, yersinia enterocolitica, serratia marcesc ...199910498727
molecular characterization of a ceftazidime-resistant morganella morganii isolate producing a tem-10 beta-lactamase. 19999989337
the use of chromagar orientation as a primary isolation medium with presumptive identification for the routine screening of urine specimens.the aim of the study was to compare the use of a novel differential culture medium chromagar, for both primary isolation and presumptive identificaton, with the method currently used in our laboratory for screening mid-stream-urine samples (msu). routine methods (rm) included blotting paper imprinting of all specimens and additional quantitative culture on cysteine lactose electrolyte-deficient agar (cled) for selected samples together with microbact 12e for further identification. the chromagar ...199910519322
regulation of inducible ampc beta-lactamase expression among enterobacteriaceae.ampc ss-lactamases are active-site serine enzymes that are primarily cephalosporinases. in many gram negative organisms, including enterobacter spp.,citrobacter freundii, serratia marcescens, morganella morganii and pseudomonas aeruginosa, the expression of chromosomal ampc genes is low but inducible in response to ss-lactams and other stimuli. the current working model for ampc induction requires exposure of bacterial cells to ss-lactam drugs or other stimuli and is linked to the cell wall recy ...199910539994
isolation, cloning, and expression of an acid phosphatase containing phosphotyrosyl phosphatase activity from prevotella intermedia.a novel acid phosphatase containing phosphotyrosyl phosphatase (ptpase) activity, designated piacp, from prevotella intermedia atcc 25611, an anaerobe implicated in progressive periodontal disease, has been purified and characterized. piacp, a monomer with an apparent molecular mass of 30 kda, did not require divalent metal cations for activity and was sensitive to orthovanadate but highly resistant to okadaic acid. the enzyme exhibited substantial activity against tyrosine phosphate-containing ...199910559178
in vivo activities of peptidic prodrugs of novel aminomethyl tetrahydrofuranyl-1 beta-methylcarbapenems.a series of novel aminomethyl tetrahydrofuranyl (thf)-1 beta-methylcarbapenems which have excellent broad-spectrum antibacterial activities exhibit modest efficacies against acute lethal infections (3.8 mg/kg of body weight against escherichia coli and 0.9 mg/kg against staphylococcus aureus) in mice when they are administered orally. in an effort to improve the efficacies of orally administered drugs through enhanced absorption by making use of a peptide-mediated transport system, several diffe ...199910049251
segmented filamentous bacteria are potent stimuli of a physiologically normal state of the murine gut mucosal immune system.segmented filamentous bacteria (sfb) are autochthonous bacteria inhabiting the intestinal tracts of many species, including humans. we studied the effect of sfb on the mucosal immune system by monoassociating formerly germfree c3h/hen mice with sfb. at various time points during 190 days of colonization, fragment cultures of small intestine and peyer's patches (pp) were analyzed for total immunoglobulin a (iga) and sfb-specific iga production. also, phenotypic changes indicating germinal center ...199910085047
cloning, sequence analyses, expression, and distribution of ampc-ampr from morganella morganii clinical isolates.shotgun cloning experiments with restriction enzyme-digested genomic dna from morganella morganii 1, which expresses high levels of cephalosporinase, into the pbkcmv cloning vector gave a recombinant plasmid, ppon-1, which encoded four entire genes: ampc, ampr, an hybf family gene, and orf-1 of unknown function. the deduced ampc beta-lactamase of pi 7.6 shared structural and functional homologies with ampc from citrobacter freundii, escherichia coli, yersinia enterocolitica, enterobacter cloacae ...199910103179
antibiotic sensitivity of bacteria associated with community-acquired urinary tract infection in britain.twelve laboratories in different parts of britain each supplied approximately 80 consecutive urinary bacterial isolates from community patients. all strains were identified by a central laboratory, where sensitivity to a variety of orally administered antimicrobials was determined by microtitre broth dilution. 65.1% of isolates were escherichia coli, 23.4% 'coliforms' other than e. coli, 4.6% proteus and morganella spp., 1.8% pseudomonas spp., 2.4% enterococci, 0.7% group b streptococci, 1.5% co ...199910511403
in vitro antibacterial activity of fk041, a new orally active cephalosporin.the in vitro activity of fk041, a new orally active cephem antibiotic, against a wide variety of clinical isolates of bacteria was investigated and compared with those of cefdinir (cfdn) and cefditoren (cdtr). fk041 exhibited broad spectrum activity against reference strains of gram-positive and gram-negative aerobes and anaerobes. fk041 was active against clinical isolates of gram-positive organisms except enterococcus faecalis with mic90s less than 1.56 microg/ml. fk041 was more active than cf ...199910513845
in vitro antibacterial activity of men 10700, a new penem antibiotic.we report here the antibacterial activity of the new penem antibiotic men 10700 against a total of 740 gram-positive and gram-negative clinical isolates, in comparison to imipenem, meropenem, cefotaxime, ceftriaxone, ciprofloxacin and gentamicin. men 10700 has shown a wide spectrum of antibacterial activity, with a potent activity both against gram-positive species (with the exception of methicillin-resistant staphylococcus aureus and staphylococcus epidermidis) as indicated by minimal inhibitor ...199910567770
molecular characterization of the genera proteus, morganella, and providencia by ribotyping.the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ...199910449462
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