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Bacterial infections of Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to gamete collecting weirs in Michigan.Herein, we describe the prevalence of bacterial infections in Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to spawn in two tributaries within the Lake Michigan watershed. Ten bacterial genera, including Renibacterium, Aeromonas, Carnobacterium, Serratia, Proteus, Pseudomonas, Hafnia, Salmonella, Shewanella and Morganella, were detected in the kidneys of Chinook salmon (n = 480) using culture, serological and molecular analyses. Among these, Aeromonas salmonicida was detected at ...201222168454
pcr detection and identification of histamine-forming bacteria in filleted tuna fish samples.total of 14 filleted yellowfin tuna fish (thunnus albacares) sold in wholesale fish market and supermarkets in milan, italy, were purchased and tested to determine microbial count, histamine level, histamine-forming bacteria, and their ability to produce histamine in culture broth. although histamine level was less than 10 ppm, many samples showed high total viable bacterial and enterobacterial counts that reached dangerous levels after temperature abuse for short periods of time. a pcr assay ta ...201222251187
species-specific dna probe and development of a quantitative pcr assay for the detection of morganella morganii.aims: the development of a sybr green quantitative pcr assay (qpcr) for the specific detection of morganella morganii, a a fish pathogen responsible of the histamine fish poisoning. methods and results: a new primer set, amplifying a 179 bp fragment of the 16s rrna gene was selected for specificity, and 14 m. morganii strains and 32 non-morganella strains were evaluated. the melting temperature of 84 °c was consistently specific for the amplicon. two standard curves were constructed: the minimum ...201222251367
antimicrobial susceptibility of gram-negative pathogens isolated from patients with complicated intra-abdominal infections in south african hospitals (smart study 2004-2009): impact of the new carbapenem breakpoints.abstract background: the study for monitoring antimicrobial resistance trends (smart) follows trends in resistance among aerobic and facultative anaerobic gram-negative bacilli (gnb) isolated from complicated intra-abdominal infections (ciais) in patients around the world. methods: during 2004-2009, three centralized clinical microbiology laboratories serving 59 private hospitals in three large south african cities collected 1,218 gnb from complicated intra-abdominal infections (ciais) and t ...201222220506
Genus-wide physicochemical evidence of extracellular crystalline silver nanoparticles biosynthesis by Morganella spp.This study was performed to determine whether extracellular silver nanoparticles (AgNPs) production is a genus-wide phenotype associated with all the members of genus Morganella, or only Morganella morganii RP-42 isolate is able to synthesize extracellular Ag nanoparticles. To undertake this study, all the available Morganella isolates were exposed to Ag+ ions, and the obtained nanoproducts were thoroughly analyzed using physico-chemical characterization tools such as transmission electron micro ...201121713008
[antimicrobial sensitive of morganella morganii].the aim of this study was the evaluation of the antimicrobial sensitive of morganella morganii rods isolated from clinical samples. this study included 50 of m. morganii strains isolated in the clinical microbiology department of dr. a. jurasz university hospital in 2008-2009. all of strains were sensitive to carbapenems (imipenem, meropenem, ertapenem, doripenem) and piperacillin/tazobactam and most of them to beta-lactam antibiotics, aminoglycosides and fluorochinolons. resistance to tetracycl ...201122184910
antibacterial activity of essential oil vapor for histamine-producing bacteria.in this study, we evaluated the antibacterial activity of essential oil vapors against histamine-producing bacteria morganella morganii nbrc3848 and raultella planticola nbrc3317. we measured the minimum inhibitory dose (mid) of 14 essential oils towards these two strains. allyl isothiocyanate (ait) and salicylaldehyde (sa) vapors showed higher antibacterial activity than the other 12 essential oil vapors. both ait and sa vapors suppressed growth of total aerobic bacteria and histamine-producing ...201122200745
finding recurrent copy number alterations preserving within-sample homogeneity.copy number alterations (cnas) represent an important component of genetic variation and play a significant role in many human diseases. development of array comparative genomic hybridization (acgh) technology has made it possible to identify cnas. identification of recurrent cnas represents the first fundamental step to provide a list of genomic regions which form the basis for further biological investigations. the main problem in recurrent cnas discovery is related to the need to distinguish ...201121873327
tumor abscess formation caused by morganella morganii complicated with bacteremia in a patient with gastrointestinal stromal tumor.we report the case of a 22-year-old man who presented with a 3-day history of watery diarrhea, abdominal pain, and fever. an image of the abdomen showed a heterogeneously echogenic mass lesion in the pelvis. the results of the blood cultures performed on admission showed the presence of morganella morganii. computed tomography-guided tube drainage was performed, and a culture of the drained abscess fluid yielded m. morganii growth. exploratory laparotomy with segmental resection of the jejunum a ...201121924697
extended-spectrum beta-lactamase orthopedic wound infections in nigeria.extended-spectrum beta-lactamase (esbl)-producing gram-negative bacteria are emerging and impacting significantly on the management of patients and hospital costs. besides, they are not being routinely sought after in diagnostic laboratories thus contributing to treatment failure.201121887050
[extracellular slime production and adhesion of morganella morganii strains to polystyrene].the aim of this study was the evaluation of the ability of extracellular slime production and adhesive properties of m. morganii strains. this study included 50 of m. morganii strains isolated from clinical samples. all of these strains were isolated in the clinical microbiology department of dr. a. jurasz university hospital in 2008-2009. five (10.0%) out of 50. m. morganii strains demonstrated extracellular slime production. adherence to polystyrene revealed 36 (72.0%) of m. morganii strains i ...201122184894
surgery in management of snake envenomation in children.snakebite is common in children especially in the developing countries. this study was undertaken to determine the role of surgery in the treatment of venomous snake bite in pediatric patients.201121877258
Clinical validation of integrated nucleic acid and protein detection on an electrochemical biosensor array for urinary tract infection diagnosis.Urinary tract infection (UTI) is a common infection that poses a substantial healthcare burden, yet its definitive diagnosis can be challenging. There is a need for a rapid, sensitive and reliable analytical method that could allow early detection of UTI and reduce unnecessary antibiotics. Pathogen identification along with quantitative detection of lactoferrin, a measure of pyuria, may provide useful information towards the overall diagnosis of UTI. Here, we report an integrated biosensor platf ...201122066011
description and plasmid characterization of qnrd determinants in proteus mirabilis and morganella morganii.clin microbiol infect abstract: we investigated the presence of qnrc and qnrd among 756 non-replicate enterobacteriaceae isolated in italy, selected for being non-susceptible to fluoroquinolones and/or resistant to third-generation cephalosporins. four proteus mirabilis and one morganella morganii (0.66% of the total) presented a qnrd gene, located in a 2687-base-pair plasmid that was entirely sequenced. the plasmid is un-typable, and contains no known coding region other than qnrd. that the qnr ...201122192340
Characterisation of histamine-producing bacteria from farmed blackspot seabream (Pagellus bogaraveo) and turbot (Psetta maxima).Turbot (Psetta maxima) and blackspot seabream (Pagellus bogaraveo) represent two of the most important emerging farmed fish species in European countries. However, no information of the presence and development of histamine-producing bacteria on them has been reported so far. Accordingly, the aim of this study was to isolate and identify the main histamine-producing bacteria in farmed turbot and blackspot seabream. For this study, 24 isolates (12 from turbot and 12 from blackspot seabream) were ...201121925757
dissemination of the rmtb gene carried on incf and incn plasmids among enterobacteriaceae in a pig farm and its environment.to investigate the prevalence and characterization of 16s rrna methylase-producing bacteria in a pig farm and its environment in east china.201121852287
Effects of wilting and molasses addition on fermentation and bacterial community in guinea grass silage.Aims: Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. Methods and Results: Direct-cut and wilted guinea grass silage was prepared with and without molasses. A high acetic acid level was observed during the fermentation of direct-cut silage, and long storage inc ...201122150390
phylogenetic characterization of bacteria in the gut of house flies (musca domestica l.).house flies (musca domestica l.) are cosmopolitan, ubiquitous, synanthropic insects that serve as mechanical or biological vectors for various microorganisms. to fully assess the role of house flies in the epidemiology of human diseases, it is essential to understand the diversity of microbiota harbored by natural fly populations. this study aimed to identify the diversity of house fly gut bacteria by both culture-dependent and culture-independent approaches. a total of 102 bacterial strains wer ...201122092755
antibacterial effect of food additives and detergents against histamine-producing bacteria on food contact material surfaces.we investigated the antibacterial activity of food additives and detergents against histamine-producing bacteria on food contact material surfaces. based on minimum inhibitory concentration (mic) testing with morganella morganii nbrc3848, raoultella planticola nbrc3317 and enterobacter aerogenes nctc10006, we screened nine food additives and four detergents with relatively high inhibitory potency. we prepared food contact material surfaces contaminated with histamine-producing bacteria, and dipp ...201122200801
Inhibitory effect of essential oils, food additives, peracetic Acid and detergents on bacterial of histidine decarboxylase.The aim of this study is to examine whether various essential oils, food additives, peracetic acid and detergents inhibit bacterial histidine decarboxylase. Crude extract of Morganella morganii NBRC3848 was prepared and incubated with various agents. Histidine decarboxylase activity was significantly inhibited (p<0.05) by 26 of 45 compounds tested. Among the 26 agents, sodium hypochlorite completely decomposed both histidine and histamine, while peracetic acid caused slight decomposition. Histid ...201122200744
polymicrobial necrotizing fasciitis involving enterobacteria producing ctx-m-15 extended-spectrum β-lactamases.necrotizing fasciitis due to multiple gram-negative organisms in a nigerian patient is described. morganella morganii and citrobacter freundii carrying the ctx-m-15 extended-spectrum β-lactamase gene were isolated, highlighting the emergence of this β-lactamase in western africa and its successful spread amongst a wider range of members of the enterobacteriaceae.201120813849
structural characterization and mhcii-dependent immunological properties of the zwitterionic o-chain antigen of morganella morganii.morganella morganii is a commensal gram-negative bacterium that has long been known to produce an antigen bearing phosphocholine groups. we determined the structure of this o-chain antigen and found that its repeating unit also contains a free amino group and a second phosphate: this alternating charge character places the m. morganii o-chain polysaccharide into a small family of zwitterionic polysaccharides (zpss) known to induce t-cell-dependent immune responses via presentation by class ii ma ...201121321054
neonatal morganella morganii sepsis: a case report and review of the literature. 201121342342
sitafloxacin: in bacterial infections.sitafloxacin is a fluoroquinolone antibacterial with in vitro activity against a broad range of gram-positive and -negative bacteria, including anaerobic bacteria, as well as against atypical pathogens. it is approved in japan for use in a number of bacterial infections caused by sitafloxacin-susceptible strains of staphylococcus spp., streptococcus pneumoniae, other streptococcus spp., enterococcus spp., moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., enterobacter sp ...201121504249
identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal rna gene sequence analysis.clin microbiol infect 2011; 17: 776-779 abstract: dna sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16s and internal transcribed spacer (its) ribosomal rna gene sequence analysis. pcr was positive for 24 of 99 samples tested. both culture and 16s rdna sequence analysis identified pseudomonas aeruginosa, streptococci and enterobacteriaceae. isolates misidentified as burkholderia cepacia by biochemical tes ...201121521414
[adhesive properties of microorganisms colonizing rheumatoid arthritis and systemic lupus erythematosus patients].evaluation of adhesive properties of microorganisms colonizing rheumatoid arthritis and systemic lupus erythematosus patients.201121598617
[beta-lactamases of clinical isolates of enterobacteriaceae family].among 62 clinical strains of enterobacteria, isolated from intestinal tract, representatives of genus escherichia made--67.4%, klebsiella--4.84%, citrobacter--4.84%, enterobacter--8.06%, proteus--6.45%, morganella of 8.06%. preparations of fluorquinolone group--gatifloxacin and moxifloxacin, the number of steady strains being from 0.0% to 33.3%, were the most effective in respect of enterobacteria the investigated strains of enterobacteria showed considerable resistance to beta-lactam antibiotic ...201121598655
new delhi metallo-ss-lactamase-1: local acquisition in ontario, canada, and challenges in detection.new delhi metallo-ß-lactamase-1 (ndm-1) is a recently identified metallo-ß-lactamase that confers resistance to carbapenems and all other ß-lactam antibiotics, with the exception of aztreonam. ndm-1 is also associated with resistance to many other classes of antibiotics. the enzyme was first identified in organisms isolated from a patient in sweden who had previously received medical treatment in india, but it is now recognized as endemic throughout india and pakistan and has spread worldwide. t ...201121624908
[urinary tract infection in patient after brain injury].intruduction: one of the most important problems in treating patients after head injury is urinary complication. the first place take urinary infections, which disturb rehabilitation process. clean intermittent catherization, bladder training and good care do not preserve infection. the purpose of our study was to present the frequency of urinary infections and main pathogens involved in these infections among patients treated in the department of rehabilitation after head injury.201121812229
new delhi metallo-ss-lactamase-1: detection and prevention. 201121788418
bacterial infection in association with snakebite: a-á10-year experience in a northern taiwan medical center.background: microbiological data of secondary wound infections following snakebites is rarely reported in taiwan. the objective of this study was to assess the secondary wound infection after venomous snakebites. methods: we conducted a 10-year retrospective survey on patients admitted for venomous snakebites and microbiological data of wound cultures at a medical center in northern taiwan. results: between april 2001 and april 2010, 231 patients who experienced snakebites were included. male pr ...201121700517
quantification of total and specific gram-negative histamine-producing bacteria species in fish using an mpn real-time pcr method.quantification of histamine-producing bacteria (hpb) is necessary in order to elucidate the role that hpb play in scombrotoxin (histamine) fish poisoning. we report here the evaluation of a real-time pcr method for the quantification of total and specific gram-negative hpb species in fish using a most probable number (mpn) format. the species-specific real-time pcr assay was 100% inclusive for independently detecting morganella morganii, enterobacter aerogenes, raoultella planticola/ornithinolyt ...201121839377
identification of bla (kpc-2) on different plasmids of three morganella morganii isolates.three morganella morganii strains resistant to carbapenems were recovered from the surgical intensive care unit (sicu) in our hospital. carbapenemases and extended-spectrum β-lactamases (esbls) were respectively detected by the modified hodge test and the modified clinical and laboratory standards institute (clsi) esbl confirmatory test in all isolates. amplification of whole-cell and plasmid dnas extracted from isolates with primers specific for the bla (kpc) produced an amplicon confirmed to b ...201121874606
first reported fatal morganella morganii infections in chickens.morganella morganii, a gram-negative rod commonly found in the intestines of humans and other animals, is here confirmed to cause a fatal infection in chickens by isolation and identification of the bacteria, 16s rrna gene sequencing, and experimental infection. this is the first case of m. morganii infection in chickens.201122176761
metallo-β-lactamases among enterobacteriaceae from routine samples in an italian tertiary-care hospital and long-term care facilities during 2008.the emergence of metallo-β-lactamase (mbl)-producing enterobacteriaceae is a serious public health concern. producers have been repeatedly isolated from patients and long-term care facility (ltcf) residents around bolzano, and we sought to assess their prevalence and clinical impact. all routine enterobacteriaceae isolates from a bolzano tertiary-care hospital and associated long-term care facilities in 2008 (n = 5500) were screened for mbls, with case details reviewed for the source patients. i ...201120345467
infected abdominal aortic aneurysm due to morganella morganii: ct findings.an infected aortic aneurysm, or mycotic aneurysm, is a rare arterial dilatation due to destruction of the infected vessel wall. common pathogens resulting in an infected aortic aneurysm are salmonella and clostridium species, as well as staphylococcus aureus; morganella morganii, on the other hand, is very rare. an infected abdominal aortic aneurysm has tendencies to grow rapidly and to rupture. the mortality rate is high in patients undergoing emergent surgical intervention. we report the case ...201120352211
development of molecular-based methods for determination of high histamine producing bacteria in fish.histamine (or scombroid) fish poisoning is a significant cause of food borne disease in the united states. in this study, we describe the development of a molecular-based technique which uses digoxigenin (dig) labeled dna probes for the detection of gram negative bacteria producing high amounts of histamine (>1000 ppm). a cocktail of pcr amplification fragments corresponding to a 709 bp fragment of the histidine decarboxylase (hdc) gene of four high producing bacteria (morganella morganii, enter ...201020392504
[bacterial meningitis due to gram-negative bacilli in adults].gram-negative bacilli are causative agents of bacterial meningitis in the neonatal period but rarely cause meningitis in adults without risk factors.201020414871
[meningoencephalitis due to morganella morganii: a case report].a central nervous system infection due to morganella morganii is uncommon. we report a case diagnosed at the neurological department of fann teaching hospital in dakar, senegal. a 12-year-old boy was hospitalized for acute meningoencephalitis. the ct scan was normal and the study of cerebrospinal fluid (csf) revealed cytological and biochemical abnormalities and m. morganii. hiv and syphilitic serologies were negative and blood cd4 lymphocyte count showed 354 per mm(3). the treatment with cefota ...201020431984
communal action of microbial cultures for red he3b degradation.the consortium pmb11 consisting of three bacterial species, originally isolated from dye contaminated soil was identified as bacillus odysseyi suk3, morganella morganii suk5 and proteus sp. suk7. the consortium possessed the ability to decolorize various textile dyes as well as mixtures of dyes. pmb11 could decolorize red he3b (50 mg l(-1)) with 99% of decolorization within 12 h in nutrient broth, while in mineral medium it could decolorize up to 97% within 24h. induction in the activities of va ...201020510505
defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions.a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ...201020537393
identification of lytic bacteriophage mmp1, assigned to a new member of t7-like phages infecting morganella morganii.mmp1 (morganella morganii phage 1) is a lytic bacteriophage newly isolated from the host bacterium m. morganii. the entire genome was sequenced, and final assembly yielded a 38,234bp linear double-stranded dna (dsdna) with a g+c content of 46.5%. in the mmp1 genome, 49 putative genes, 10 putative promoters and 2 predicted sigma-independent terminators were determined through bioinformatic analysis. a striking feature of the mmp1 genome is its high degree of similarity to the t7 group of phages. ...201020561579
[unusual cause of infant severe community-acquired pneumonia: morganella morganii]. 201020570476
phylogenetic analysis of the genera proteus, morganella and providencia by comparison of rpob sequences of type and clinical strains suggests the classification of proteus myxofaciens in a new genus cosenzaea gen. nov., as cosenzaea myxofaciens comb. nov.phylogenetic analysis of partial rpob gene sequences of type and clinical strains belonging to different 16s rrna gene-fingerprinting ribogroups within 11 species of enterobacteria of the genera proteus, morganella, and providencia allowed to define rpob clades supported by high bootstrap values and confirmed by >/=2.5% nucleotide divergence. no rpob clade included strains belonging to different species and the majority of the species were confirmed as discrete and homogeneous, since they consti ...201020709916
seasonal variation, chemical composition, and analgesic and antimicrobial activities of the essential oil from leaves of tetradenia riparia (hochst.) codd in southern brazil.the seasonal variation of the chemical composition of the essential oil from fresh leaves of tetradenia riparia (hochst.) codd grown in southern brazil was analyzed by gc-ms, and the analgesic and antimicrobial activities of this oil were assayed. the yield of essential oil ranged from 0.17% to 0.26%, with the maximum amount in winter and the minimum in spring. the results obtained from principal components analysis (pca) revealed the existence of high chemical variability in the different seaso ...201020714310
morganella morganii pericarditis in a child with x-linked agammaglobulinemia. 201020723125
prospective study of urinary tract infection surveillance after kidney transplantation.urinary tract infection (uti) remains one of the main complications after kidney transplantation and it has serious consequences.201020723254
first case of capd-related peritonitis caused by morganella morganii.morganella morganii, a gram-negative bacillus, is a rare cause of peritonitis. in this article we report a 55-year-old female patient with peritonitis due to morganella morganii who was receiving continuous ambulatory peritoneal dialysis (capd).201020056997
histamine, cadaverine, and putrescine produced in vitro by enterobacteriaceae and pseudomonadaceae isolated from spinach.a total of 364 bacterial isolates, obtained from spinach leaves, were assayed in a decarboxylase broth containing histidine, lysine, and ornithine to check their ability to produce biogenic amines, and then quantified by high-performance liquid chromatography. among these isolates, 240 formed cadaverine, 208 formed putrescine, and 196 formed histamine, in widely varying amounts. they frequently produced more than one biogenic amine. klebsiella pneumoniae subsp. pneumoniae and morganella morganii ...201020132689
differential characterization of biogenic amine-producing bacteria involved in food poisoning using maldi-tof mass fingerprinting.histamine poisoning is caused by the consumption of fish and other foods that harbor bacteria possessing histidine decarboxylase activity. with the aim of preventing histamine formation, highly specific mass spectral fingerprints were obtained from the 16 major biogenic amine-producing enteric and marine bacteria by means of maldi-tof ms analysis. all bacterial strains analyzed exhibited specific spectral fingerprints that enabled its unambiguous differentiation. this technique also identified p ...201020151397
the conundrum of the gram-positive rod: are we missing important pathogens in complicated skin and soft-tissue infections? a case report and review of the literature.the designation "gram-positive bacillus" includes a variety of pleomorphic microorganisms, including diphtheroids, coryneform species, coccobacilli, and other small rods. despite differing greatly in their virulence, sources, and even genus, these microscopically similar organisms are often difficult to differentiate without genetic testing.201019803730
antibacterial activities of crude extract of aloe barbadensis to clinically isolated bacterial pathogens.the antibacterial activity of aloe barbadensis was tested on clinically isolated bacterial pathogens i.e. enterococcus bovis, staphylococcus aureus, escherichia coli, proteus vulgaris, proteus mirabilis, pseudomonas aeruginosa, morganella morganii, and klebsiella pneumoniae causing infection in human being. ethanolic and aqueous extracts were used for the antibacterial effect, which was measured by the appearance of zone of inhibition. relatively higher mic concentrations were obtained for gram ...201019263248
screening of the constituents, antimicrobial and antioxidant activity of endemic origanum hypericifolium o. schwartz & p.h. davis.the chemical compositions, total phenol content, antioxidant and antimicrobial activities with oxidant status of the essential oil from an endemic turkish species, origanum hypericifolium, were investigated. steam distillation (sd) was used to isolate the essential oils, and the chemical analyses were performed by gas chromatography-mass spectrometry (gc-ms). the antimicrobial activity was tested by agar disc diffusion method against morganella morganii (clinic isolate), micrococcus flavus (clin ...201020835958
new delhi metallo-beta lactamase (ndm-1) in enterobacteriaceae: treatment options with carbapenems compromised.carbapenems are among the few useful antibiotics against multidrug resistant gram negative bacteria particularly those with extended spectrum beta lactamase. however resistance to carbapenems occurs and is mediated by mechanisms like loss of outer membrane proteins and production of beta lactamase that is capable of hydrolyzing carbapenems. an alert issued in the uk in 2009 warned of an increasing number of carbapenem resistant enterobacteriaceae strains identified in uk hospital patients. many ...201020848811
detection and pfge analysis of esbl-producing isolates of proteus species isolated from patients at tehran hospitals.the production of extended-spectrum β-lactamases (esbls), in particular tem and ctx-m type, by the strains of proteus spp. is a contributing factor to the emergence of bacterial drug resistance. the aim of this study was to screen and determine of esbls genes among strains of proteus spp. at tehran hospitals.201020885345
diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania.aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ...201020941581
[viable non-culturable bacteria].viable but non-culturable cells (vbnc) are defined as live bacteria, but which do not either grow or divide. such bacteria cannot be cultivated on conventional media (they do not form colonies on solid media, they do not change broth appearance), but their existence can be proved using other methods. the switch to the vbnc stage has been described and documented for several bacterial species: vibrio spp. (cholerae, vulnificus and other species), escherichia coli (including ehec), campylobacter j ...201021038700
comparison of bacterial diversity in wheat bran and in the gut of larvae and newly emerged adult of musca domestica (diptera: muscidae) by use of ethidium monoazide reveals bacterial colonization.the objective of the current study is to investigate the bacterial colonization within the gut of the house fly, musca domestica l. (diptera: muscidae), at the larval stage and the bacterial community of the gut of the house fly at the newly emerged adult stage. after using ethidium monoazide to inhibit recovery of nucleic acids from dead bacteria, three polymerase chain reaction (pcr)-amplified 16s rdna libraries from wheat bran, larvae, and newly emerged adults was constructed, analyzed, and c ...201021061987
predation of human pathogens by the predatory bacteria micavibrio aeruginosavorus and bdellovibrio bacteriovorus.the focus of this study was to evaluate the potential use of the predatory bacteria bdellovibrio bacteriovorus and micavibrio aeruginosavorus to control the pathogens associated with human infection.201021114596
laboratory detection of extended-spectrum-beta-lactamase-producing enterobacteriaceae: evaluation of two screening agar plates and two confirmation techniques.the worldwide prevalence of extended-spectrum-beta-lactamase-producing esbl-producing enterobacteriaceae (esbl-e) is increasing, making the need for optimized detection techniques more urgent. in this study we investigated the performance of two esbl-e screening and two esbl-e confirmation techniques. in accordance with the dutch national guidelines (www.wip.nl), a collection of 642 highly resistant enterobacteriaceae strains, as identified by vitek2, was used to test the performances of two scr ...201021123527
recurrent lower limb abscesses as the primary manifestation of aorto-femoral graft paraprosthetic fistula.a 62-year-old man was presented to our facility with recurrent right lower limb abscesses. he had an aortobifemoral graft for an aortoiliac occlusive disease. the diagnosis of paraprosthetic fistula was confirmed by performing a high-resolution contrast-enhanced computed tomography, whereas labeled leukocyte imaging provided a negative result. the graft present in the right limb was removed and extra-anatomical femoro-femoral bypass was performed along with segmental ileal resection of the affec ...201021126852
bacterial kinetics-controlled shape-directed biosynthesis of silver nanoplates using morganella psychrotolerans.we show for the first time that by controlling the growth kinetics of morganella psychrotolerans, a silver-resistant psychrophilic bacterium, the shape anisotropy of silver nanoparticles can be achieved. this is particularly important considering that there has been no report that demonstrates a control over shape of ag nanoparticles by controlling the growth kinetics of bacteria during biological synthesis. additionally, we have for the first time performed electrochemistry experiments on bacte ...201021142094
morganella morganii could be an important intensive care unit pathogen. 201021253351
biodegradation studies and sequencing of microcystin-lr degrading bacteria isolated from a drinking water biofilter and a fresh water lake.the presence of microcystin-lr -degrading bacteria in an active anthracite biofilter and in lake mead, nevada was investigated. four bacterial isolates from enrichment culture were identified using 16s rrna analysis. microcystin biodegradation tests were performed with both, the enrichment cultures and the respective isolates, using microcystin alone and acetate as carbon sources. a newly recognized microcystin-degrading bacterium, morganella morganii, was isolated from the biofilter and from la ...201020219514
development of a real-time pcr assay with an internal amplification control for detection of gram-negative histamine-producing bacteria in fish.prompt detection of bacteria that contribute to scombrotoxin (histamine) fish poisoning can aid in the detection of potentially toxic fish products and prevent the occurrence of illness. we report development of the first real-time pcr method for rapid detection of gram-negative histamine-producing bacteria (hpb) in fish. the real-time pcr assay was 100% inclusive for detecting high-histamine producing isolates and did not detect any of the low- or non-histamine producing isolates. the efficienc ...201021356438
extended spectrum beta-lactamase detection in gram-negative bacilli of nosocomial origin.resistance to third generation cephalosporins by acquisition and expression of extended spectrum beta lactamase (esbl) enzymes among gram-negative bacilli is on a rise. the presence of esbl producing organisms significantly affects the course and outcome of an infection and poses a challenge to infection management worldwide.200920300397
catheter-related urinary tract infection in patients suffering from spinal cord injuries.urinary tract infection is commoner in patients with spinal cord injuries because of incomplete bladder emptying and the use of catheters that can result in the introduction of bacteria into the bladder. 145 patients suffering from spinal cord injuries, admitted to the institute for physical medicine and rehabilitation, centre for paraplegia of the clinical centre of the university of sarajevo, were included. the patients were divided in three groups according to the method of bladder drainage: ...200919284388
separation of viable histamine-producing bacteria from yellowtail meat components by density gradient centrifugation.quantitative separation of live cells from food samples is essential for non-culture methods to be validated. in this viewpoint, the feasibility of density gradient centrifugation (dgc) was demonstrated for the first time using samples of yellowtail meat to which morganella morganii, a histamine producing bacterium had been added. using a ficoll density gradient from 50 to 10 w/v % with 10 w/v % steps, meat-free fractions of m. morganii cells were collected in 20-50 w/v % layers. the total cell ...200919344096
[ability of opportunistic enterobacteria to adapt to different temperatures].to study variability of enzymatic apparatus of opportunistic enterobacteria.200919459471
antimicrobial activity of selected plant species from "the argentine puna" against sensitive and multi-resistant bacteria.the plant species reported here are traditionally used in the "puna" or "altiplano" of argentina for ailments related to bacterial infections. the aim of this study was to evaluate their antimicrobial properties against a panel of sensitive and multi-resistant gram-positive and gram-negative bacteria.200919467313
haemorrhagic bullae associated with a chicken scratch.complicated skin and soft tissue infection caused by a chicken scratch has rarely been reported. a 3-year-old boy who developed haemorrhagic bullae and necrotising fasciitis following a chicken scratch is reported. morganella morganii and enterococci were cultured from the aspirate. he received antimicrobial treatment, surgical debridement and a skin graft, and recovered uneventfully.200919941755
tubo-ovarian abscess with morganella morganii bacteremia.tubo-ovarian abscess caused by morganella morganii is unusual. a 54-year-old menopausal woman visited the emergency room with lower abdominal pain, vomiting, and fever for 4 days. pelvic examination revealed lifting tenderness over the right adnexum without motion tenderness of the uterus. pelvic sonogram revealed a cystic lesion with heterogeneous content in the right ovary. salpingo-oophoritis was suspected. clindamycin and gentamicin were administered intravenously after obtaining blood cultu ...200919949761
[fatal neonatal sepsis caused by vertical transmission of morganella morganii. report of one case].we report a term neonate who developed early-onset sepsis due to morganella morganii. the child was vaginally delivered after a short labor, and presented signs of perinatal asphyxia. blood cultures taken soon after birth and from mother's lochia were positive for this microorganism. the infection was unresponsive to treatment with cefotaxime, to which the microorganism was susceptible, and the infant died at 17 days of age. m morganii is an opportunistic and uncommon pathogen, causing disease m ...200920011962
18-f fluorodeoxyglucose positron emission tomography indicating unsuspected infections in two patients with dermatomyositis.dermatomyositis is an idiopathic inflammatory myopathy that may be associated with malignancies. the technique of 18-f fluorodeoxyglucose (fdg)-positron emission tomography (pet) is an important tool to investigate underlying malignancy in patients with a possible paraneoplastic syndrome. we report two consecutive patients with dermatomyositis in whom 18-f fdg-pet revealed unsuspected infections. physicians should be aware that a positive 18-f fdg-pet is not specific for malignancy and may revea ...200920055842
antibiotic resistance of gram negatives isolates from loggerhead sea turtles (caretta caretta) in the central mediterranean sea.previous studies on fish and marine mammals support the hypothesis that marine species harbor antibiotic resistance and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to assess the resistance to antimicrobial agents of gram negative strains isolated from loggerhead sea turtles (carettacaretta). oral and cloacal swabs from 19 live-stranded loggerhead sea turtles, with hooks fixed into the gut, were analyzed. the antimicrobial resistance ...200919473669
design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties.the aims of the present study were to determine the antibacterial and antifungal activity as well as mutagenicity of sechium edule fluid extract and to obtain a pharmaceutical formulation with them. the extract exhibited antimicrobial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, serratia marcescens, morganella morganii, acinetobacter baumannii, pseudomonas aeruginosa, stenotrophomonas maltophilia, candida spp. and aspergillus spp. isolated fr ...200919477252
oral bacterial flora of the chinese cobra (naja atra) and bamboo pit viper (trimeresurus albolabris) in hong kong sar, china.to determine the oral bacterial flora associated with two common local venomous snakes in hong kong, namely the chinese cobra (naja atra) and the bamboo pit viper (trimeresurus albolabris).200919494373
fatal morganella morganii bacteraemia in a diabetic patient with gas gangrene.we report a case of a 60-year-old lady with a history of a heel ulcer that had not responded to antibiotic therapy. this progressed to involve the right leg, which was swollen and erythematous. radiological imaging revealed the presence of gas within the fascial planes. blood cultures on admission yielded morganella morganii. due to the extent of the gas gangrene and her co-morbidities the patient was not suitable for surgical intervention and was treated conservatively with antibiotics. she det ...200919502368
[evaluation of effectiveness and tolerance of treatment with azithromycin 1.5% eye drops in bacterial conjunctivitis].azithromycin is a macrolide class antibiotic, recently adapted for topical use in ophthalmology. it is effective against the most frequent pathogens found in bacterial conjunctivitis, gram positive and gram negative bacteria. azithromycin has the specificity to have sustained high tissue levels: after repeated instillation, it has been shown to reach sustained concentrations above the mics of susceptible bacteria for 4 days in tears and for 7 days in conjunctiva.200919517846
crystalline bacterial biofilm formation on urinary catheters by urease-producing urinary tract pathogens: a simple method of control.the problem of catheter encrustation stems from infection by urease-producing bacteria. these organisms generate ammonia from urea, elevate the ph of urine and cause crystals of calcium and magnesium phosphates to form in the urine and the biofilm that develops on the catheter. in this study, a laboratory model was used to compare the ability of 12 urease-positive species of urinary tract pathogens to encrust and block catheters. proteus mirabilis, proteus vulgaris and providencia rettgeri were ...200919556373
investigation of shell disease in map turtles (graptemys spp.).nineteen map turtles (graptemys spp.) maintained under natural conditions were investigated because of chronic shell abnormalities. animals were evaluated using a novel shell scoring system that divided the 54 scutes into six regions, with each region scored for lesion extent and severity, and summated to produce a total shell disease score (tsds). complete blood counts and various biochemistry analytes (total protein, albumin, globulin, urea, uric acid, 25-hydroxycholecalciferol, phosphorus, an ...200919617474
[multicenter study on the monitoring of in vitro susceptibility to tigeeyeline in santiago, chile].the objective of this multicenter study was to determine tigecycline susceptibility rates, measured by agar diffusion, in nine hospitals in santiago and to compare these rates with other antimicrobials. each center studied 20 strains per month. all intermediate and fully resistant strains as well as 10% of susceptibile strains were also studied by the broth microdilution method. overall, 2301 strains were studied displaying the following susceptibility rates for tigecycline: 100% for streptococc ...200919621154
identification of a modular pathogenicity island that is widespread among urease-producing uropathogens and shares features with a diverse group of mobile elements.pathogenicity islands (pais) are a specific group of genomic islands that contribute to genomic variability and virulence of bacterial pathogens. using a strain-specific comparative genomic hybridization array, we report the identification of a 94-kb pai, designated icepm1, that is common to proteus mirabilis, providencia stuartii, and morganella morganii. these organisms are highly prevalent etiologic agents of catheter-associated urinary tract infections (cauti), the most common hospital acqui ...200919687197
[formation of biofilms by fecal strains of enterobacteria and yeast fungi from candida genus].to assess the ability of bacteria and candida genus' yeast fungi from human fecal microflora to form biofilms.200919715202
extended-spectrum beta-lactamases among enterobacteriaceae isolated in a public hospital in brazil.extended-spectrum beta-lactamases (esbl) in enterobacteria are recognized worldwide as a great hospital problem. in this study, 127 esbl-producing enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at são paulo, brazil, were submitted to analysis by pcr with specific primers for bla shv, bla tem and bla ctx-m genes. from the 127 isolates, 96 (75.6%) klebsiella pneumoniae, 12 (9.3%) escherichia coli, 8 (6.2%) morganella morganii, 3 (2.3%) proteus ...200919739000
needle puncture and transcutaneous bone biopsy cultures are inconsistent in patients with diabetes and suspected osteomyelitis of the foot.needle puncture has been suggested as a method for identifying bacteria in the bones in patients with diabetes with osteomyelitis of the foot. however, no studies have compared needle puncture with concomitant transcutaneous bone biopsy, which is the current standard recommended in international guidelines.200919228109
[susceptibility of enterobacteriaceae rods to selected tetracyclines].the aim of this study was to evaluated the susceptibility of enterobacteriaceae rods to tigecycline, tetracycline and doxycycline. we analysed 121 strains belonged to species as follows: escherichia coli (40 strains), proteus mirabilis (22), enterobacter cloacae (19), klebsiellapneumoniae (18), morganella morgannii (13), serratia marcescens (4), klebsiella oxytoca (3) i proteus vulgaris (2). all strains of e. coli, k. oxytoca, s. marcescens and p. vulgaris were susceptible to tigecycline. twenty ...200920201321
[epidemiology of bacterial intrahospital infections in newborns].nosocomial infections are important problem for the modern medicine. especially sensitive category, susceptible to nosocomial infections, are the infants. the aim of the research was to establish an active monitoring over the breaking out of the nosocomial infections in the neonatology department of the gynecology and obstetrics clinic of the university clinical center tuzla (ukc) and to determine the kind of bacteria that causes the nosocomial infections as well as the type of infection accordi ...200819469274
early and late endophthalmitis following trabeculectomy in a chinese population.to investigate the rate of bleb-related endophthalmitis over 5 years in a chinese population. methods.:retrospective chart review.200818203087
phylogenetic relationships among species and genera of lycoperdaceae based on its and lsu sequence data from north european taxa.phylogenetic relationships, limits of species, and genera within lycoperdaceae, were inferred by use of its and lsu nu-rdna sequence data. lycoperdaceae was confirmed as monophyletic, and mycenastrum corium as a sister taxon to the ingroup. four major clades were identified and received weak to moderate support and correspond with the genera lycoperdon, bovista, calvatia, and disciseda. the lycoperdon clade includes species from lycoperdon, vascellum, morganella, handkea, bovistella, and calvati ...200818207380
chemical markers for the evaluation of raw material hygienic quality in egg products.the aim of this research was to study uracil and lactic and acetic acids as chemical markers for hygienic quality evaluation of raw material in liquid pasteurized egg products. uracil, absent in sound whole eggs, was formed in raw and pasteurized egg products as a consequence of high microbial contamination (>10(6) cfu/g) after a sufficient lag time, remaining stable at 4 degrees c but disappearing after 7 days of storage at 25 degrees c. both lactic and acetic acids, starting from initial value ...200818211016
the gut-brain barrier in major depression: intestinal mucosal dysfunction with an increased translocation of lps from gram negative enterobacteria (leaky gut) plays a role in the inflammatory pathophysiology of depression.there is now evidence that major depression (mdd) is accompanied by an activation of the inflammatory response system (irs) and that pro-inflammatory cytokines and lipopolysacharide (lps) may induce depressive symptoms. the aim of the present study was to examine whether an increased gastrointestinal permeability with an increased translocation of lps from gram negative bacteria may play a role in the pathophysiology of mdd. toward this end, the present study examines the serum concentrations of ...200818283240
diverse antimicrobial killing by enterococcus faecium e 50-52 bacteriocin.an effective bacteriocin was identified and characterized. lactic acid bacteria were screened against campylobacter jejuni. one bacteriocin producer, enterococcus faecium (nrrl b-30746), was studied. the isolate was grown, and the bacteriocin was purified to single-band homogeneity. biochemical traits indicated that the peptide was a class iia bacteriocin, and it was named e 50-52. the bacteriocin had a molecular weight of 3339.7 and an isoelectric point of 8.0. the minimal inhibitory concentrat ...200818293921
surveillance for plasmid-mediated quinolone resistance determinants in enterobacteriaceae within the calgary health region, canada: the emergence of aac(6')-ib-cr.a study was designed to determine the prevalence of plasmid-mediated quinolone resistance (pmqr) determinants among clinical isolates of enterobacteriaceae from the calgary health region (chr).200818296438
morganella morganii-associated arthritis in a diabetic patient.this case report involves a 60-year-old diabetic man who developed septic arthritis as a result of the pathogen morganella morganii. the patient had complaints of elevated body temperature, malaise, rigors and pain in the left knee, despite no history of trauma. on examination of the knee, erythema, warmth, tenderness and swelling was observed. arthrocentesis performed on his left knee indicated the presence of straw-coloured, cloudy fluid without crystals. bacterial identification based on bioc ...200818327547
secondary structure of its2 rrna provides taxonomic characters for systematic studies--a case in lycoperdaceae (basidiomycota).the secondary structure of the its2 rdna transcript (pre-rrna) could provide information for identifying homologous nucleotide characters useful for cladistic inference of relationships. such structure data could become taxonomic characters. this work compares the effect of several modern nucleotide alignment strategies, including those making use of structure data, on phylogenetic inference. from both the phylogenetic analyses and comparative secondary structure, implications for taxonomy and e ...200818342242
characterization of a second ornithine decarboxylase isolated from morganella morganii.the genes involved in the putrescine formation by morganella morganii were investigated because putrescine is an indicator of food process deterioration. we report here on the existence of a new gene for ornithine decarboxylase (odc) in m. morganii. the sequenced 5,311-bp dna region showed the presence of four complete and one partial open reading frame. putative functions have been assigned to several gene products by sequence comparison with the proteins included in the databases. the third op ...200818389719
in vitro interaction between mecillinam and piperacillin-tazobactam in the presence of azithromycin against members of the enterobacteriaceae family and pseudomonas aeruginosa.mecillinam was tested in vitro alone or in combination with piperacillin-tazobactam and azithromycin against representative species of the enterobacteriaceae family and pseudomonas aeruginosa to extend its antibacterial spectrum, and to protect mecillinam from inactivating enzymes taking advantage of the presence of tazobactam. drug interactions were studied by microdilution method, by selection of spontaneous resistant mutants on agar plates containing the drugs in combination and by time kill ...200818437840
[case of the month; purple urine bag syndrome (pubs)]. 200818460735
effects of rifaximin on bacterial virulence mechanisms at supra- and sub-inhibitory concentrations.rifaximin, a poorly absorbed rifamycin derivative, exhibited time-dependent bactericidal activity and at concentrations as low as 1/32 of the minimum inhibitory concentration (mic) caused morphological alterations in both susceptible and resistant bacterial strains. spontaneous rifaximin-resistant clones appeared with an incidence of 2.6 x 10(-7). the percentage of escherichia coli cells cured of various plasmids ranged from: 4.5-70% (flac), 0-18% (pbp507), 7.7-43.8% (plasmid carrying esbl genes ...200818467244
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