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comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study.the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ...200111448564
source and identification of histamine-producing bacteria from fresh and temperature-abused albacore.histamine-producing bacteria were isolated from fresh and temperature-abused albacore using two different isolation procedures. typically, the bacterial isolates on niven's or modified niven's medium produced negligible or low levels of histamine (<300 ppm) in histamine enumeration broth. the most frequently found species using this approach was hafnia alvei. by prescreening on selective media (eosin methylene blue [emb] agar for enteric bacteria; deman rogosa sharpe agar for lactic acid bacteri ...200111456189
necrotizing fasciitis in a newborn infant: a case report.we report the case of a one-day-old newborn infant, female, birth weight 1900 g, gestational age 36 weeks presenting with necrotizing fasciitis caused by e. coli and morganella morganii. the newborn was allowed to fall into the toilet bowl during a domestic delivery. the initial lesion was observed at 24 hours of life on the left leg at the site of the venipuncture for the administration of hypertonic glucose solution. despite early treatment, a rapid progression occurred resulting in a fatal ou ...200111460206
comparative antimicrobial spectrum and activity of bms284756 (t-3811, a desfluoroquinolone) tested against 656 enterobacteriaceae, including preliminary in vitro susceptibility test comparisons and development.bms284756 (t-3811), a novel des-f(6)-quinolone, was evaluated using isolates of enterobacteriaceae from the sentry antimicrobial surveillance program tested by etest (ab biodisk, solna, sweden), reference broth microdilution and disk diffusion (5-microg) methods. ciprofloxacin, levofloxacin, gemifloxacin and gatifloxacin were also tested by broth microdilution as comparator antimicrobial agents within the same drug class. the 656 isolate collection included species from the genera citrobacter, e ...200111516937
early onset mixed morganella and klebsiella sepsis in a neonate.a premature baby girl was delivered vaginally to a mother who had an otherwise normal pregnancy, and spontaneous premature onset of labour. she had early onset neonatal sepsis with pneumonia. the baby's blood culture as well as the amniotic membrane culture grew morganella and klebsiella. she recovered on appropriate antibiotics. this is only the second reported case of early onset neonatal sepsis due to morganella. the literature is reviewed.200111519292
intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae.intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ...200111544246
gram-negative bacterial toe web infection: a survey of 123 cases from the district of cagliari, italy.foot intertrigo is mostly caused by dermatophytes and yeasts, less frequently by gram-positive and gram-negative bacteria. nevertheless, the importance of polymicrobial infections and especially colonizations of pseudomonas aeruginosa can cause therapy problems in relation to antibiotic resistance and the risk of potentially lethal complications.200111568743
antibiotic treatment to prevent urinary tract infections after urodynamic evaluation.the aim of the study was to determine the efficacy of cotrimoxazole administration after urodynamic testing to prevent urinary tract infections. in a single-blind prospective randomized study 94 women who attended for urodynamic evaluation were included. after multichannel urodynamic testing, including two catheterizations, the women received a single dose of cotrimoxazole or placebo. a clean-catch urine specimen was tested for infection after 1 week. seventy women returned a urine specimen afte ...200111569654
identification of bacteria crucial to histamine accumulation in pacific mackerel during storage.bacterial growth and histamine formation in pacific mackerel during storage at 0, 4, 15, and 25 degrees c were monitored. to identify bacterial species contributing to histamine formation, several groups of bacteria were isolated by using selective media under temperatures corresponding to the various storage conditions. initially, low counts of bacteria were found in the gill, skin, and intestine of fresh fish, and only weak histamine formers were found in the gill. histamine was found in the m ...200111601705
cefepime, piperacillin-tazobactam, and the inoculum effect in tests with extended-spectrum beta-lactamase-producing enterobacteriaceae.there is little information about the clinical effectiveness of cefepime and piperacillin-tazobactam in the treatment of infections caused by extended-spectrum beta-lactamase (esbl)-producing pathogens. some inferences have been drawn from laboratory studies, which have usually involved only one or a few strains of esbl-producing klebsiella pneumoniae or escherichia coli that produced only a limited range of esbls. such studies are indirect, sometimes conflicting, indicators of efficacy. to exte ...200111709338
human v gamma 2v delta 2 t cells produce ifn-gamma and tnf-alpha with an on/off/on cycling pattern in response to live bacterial products.whereas cytokine production in alphabeta t cells is rapidly regulated by exposure to peptide ag, the mechanisms regulating cytokine production by gammadelta t cells are unknown. in this study, we demonstrate that human vgamma2vdelta2 t cells produce ifn-gamma and tnf-alpha as early as 2 h after ag exposure, and that they produce these cytokines in a dose- and time- dependent manner in response to stimulation with a live bacterial product, iso-butylamine (iba), but not to dead bacteria or lps. ga ...200111714780
clinical picture: bacterial translocation in neutropenic sepsis. 200111741622
growth and histamine formation of morganella morganii in determining the safety and quality of inoculated and uninoculated bluefish (pomatomus saltatrix).the objective of this study was to determine the effect of normal microflora and morganella morganii on histamine formation and olfactory acceptability in raw bluefish under controlled storage conditions. fillets inoculated with and without m. morganii were stored at 5, 10, and 15 degrees c for 7 days. microbial isolates from surface swabs were identified and screened for histidine decarboxylase activity. olfactory acceptance was performed by an informal sensory panel. histamine levels were quan ...200111770632
[bacterial causes of meningitis in newborns].bacterial meningitis jeopardizes the life of affected newborns and in survived children often leaves permanent sequels. the sooner the diagnosis is established and the therapy is started, the more a prosperous outcome can be expected. when the indication is established, lumbar punction should be performed. on the basis of the results of cytological and biochemical analyses of the cerebrospinal fluid (csf) that point to bacterial meningitis, the initial therapy should begin even before the result ...200115637989
biogenic amines produced by enterobacteriaceae isolated from meat products.biogenic amines in ground meat and processed meat products are one of the indicators to determine the poor quality raw material. major histamine forming bacterium was escherichia coli (strain ec04 with 65.88 mg/100 ml in brain heart infusion medium), followed by the microorganisms morganella morganii (strain mm4 with 8.40 mg/100 ml and strain mm7 with 8.28 mg/100 ml) and proteus mirabilis (strain pm02 with 8.76 mg/100 ml), respectively. the highest putrescine production level was found in citrob ...200122062111
antibacterial effect of human v gamma 2v delta 2 t cells in vivo.v gamma 2v delta 2 cells, a class of t cells found only in primates, are reactive to nonpeptide organophosphate and alkylamine antigens secreted by bacteria and parasites. these cells make up 2-5% percent of human peripheral blood t cells but expand to make up 8-60% of peripheral blood t cells during bacterial and parasitic infections. we show here, using a chimeric severe combined immunodeficiency (scid) mouse (hu-scid) model, that human v gamma 2v delta 2 t cells mediate resistance to extracel ...200111696580
fatal septicemia and meningitis due to morganella morganii in a patient with hodgkin's disease.a unique case of spontaneous morganella morganii meningitis in a patient with stage iv hodgkin's disease, following hematogenous spread from the urinary tract, is described. late initiation of appropriate antibiotic treatment was probably responsible for the fatal outcome. this case illustrates the pathogenic potential of m. morganii in immunocompromised hosts.200111515771
haemagglutination, haemolysin production and serum resistance of proteus and related species isolated from clinical sources.a total of 148 strains of proteus and related species comprising of proteus mirabilis (116), proteus vulgaris (24), providentia rettgeri (4), providentia alcalifaciens (2), providentia stuarti (1) and morganella morganii (1), isolated from various sources, were examined for haemagglutination (ha), haemolysin production (hl) and serum resistance (sr). maximum isolates were obtained from urine (47.30%) and pus (40.54%) and they were multidrug resistant. the sensitivity to ciprofloxacin was 78.38%, ...200117664798
chemical composition and cytotoxic and antimicrobial activity of calycotome villosa (poiret) link leaves.the chemical composition of the essential oil and methanol extract of calycotome villosa (poiret) link leaves collected in sardinia (italy) has been studied by analytical and spectroscopic methods. falcarinol and some alcohols, terpenes, furan derivatives, and paraffins have been isolated from the essential oil. thirteen alkaloids and falcarinol have been identified in the chloroform fraction of the basic methanol extract. six flavonoids and four anthraquinones have been isolated in the chlorofo ...200111482772
acid phosphatase/phosphotransferases from enteric bacteria.we have investigated the enzymatic phosphorylation of nucleosides and found that morganella morganii phoc acid phosphatase exhibits regioselective pyrophosphate (pp(i))-nucleoside phosphotransferase activity. in this study, we isolated genes encoding an acid phosphatase with regioselective phosphotransferase activity (ap/ptase) from providencia stuartii, enterobacter aerogenes, escherichia blattae and klebsiella planticola, and compared the primary structures and enzymatic characteristics of the ...200116233057
quantum mechanical study of the intermediates formed following the reaction of the histidine decarboxylase's substrate and inhibitors with coenzyme.histidine decarboxylase catalyses the decarboxylation of l-histidine to histamine using pyridoxal-5'-phosphate (plp) as coenzyme. the pm3 quantum mechanical conformation method of analysis and heat of formation calculation were carried out for intermediates which are probably formed during the interaction of histidine (substrate), (s)-alpha-methylhistidine, (s)-alpha-hydrazinohistidine, (s)-alpha-fluoromethylhistidine and (s)-alpha-difluoromethylhistidine (inhibitors) with plp-dependent histidin ...200010785554
isolation of bacteria from toxic dinoflagellate alexandrium minutum and their effects on algae toxicity.attempts were made to isolate the bacteria from toxic dinoflagellate alexandrium minutum t1 and to study the effect of these bacteria on the growth and toxicity of a. minutum t1. it was found that intracellular bacterial species including pasteurella haemolytica, pseudomonas vesicularis, and sphingomonas sp., and extracellular bacterial species including pasteurolla pneumotropica, morganella wisconsensis, flavobacterium oryzihabitans, pseudomonas pseudomallei, and sphingomonas sp. all of them we ...200011126517
natural antibiotic resistance of bacteria isolated from larvae of the oil fly, helaeomyia petrolei.helaeomyia petrolei (oil fly) larvae inhabit the asphalt seeps of rancho la brea in los angeles, calif. the culturable microbial gut contents of larvae collected from the viscous oil were recently examined, and the majority (9 of 14) of the strains were identified as providencia spp. subsequently, 12 of the bacterial strains isolated were tested for their resistance or sensitivity to 23 commonly used antibiotics. all nine strains classified as providencia rettgeri exhibited dramatic resistance t ...200011055901
the hybrid-cluster protein ('prismane protein') from escherichia coli. characterization of the hybrid-cluster protein, redox properties of the [2fe-2s] and [4fe-2s-2o] clusters and identification of an associated nadh oxidoreductase containing fad and [2fe-2s].hybrid-cluster proteins ('prismane proteins') have previously been isolated and characterized from strictly anaerobic sulfate-reducing bacteria. these proteins contain two types of fe/s clusters unique in biological systems: a [4fe-4s] cubane cluster with spin-admixed s = 3/2 ground-state paramagnetism and a novel type of hybrid [4fe-2s-2o] cluster, which can attain four redox states. genomic sequencing reveals that genes encoding putative hybrid-cluster proteins are present in a range of bacter ...200010651802
a kinetic study on the interaction between tazobactam (a penicillanic acid sulphone derivative) and active-site serine beta-lactamases.the interaction between tazobactam and several chromosome- and plasmid-encoded (tem, shv, pse types) class a and c beta-lactamases was studied by spectrophotometry. tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner beta-lactam. a detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum beta-lactamases; the presence of a turn-over of acyl-enzyme ...200010850951
[the structure and antibiotic sensitivity of the causative agents of community-acquired infectious diseases of bacterial origin in children].four hundred and forty pediatric patients at the age of 7 days to 15 years with various infections admitted to the hospital within a month were examined. the biological material was inoculated to blood agar on the first days of the patient admittance to the hospital and after the growth the organisms were isolated and identified. antibiotic susceptibility of the isolates was assayed with the disk diffusion method. 479 strains in all were tested. the most frequent cases requiring hospitalization ...200010768061
palliative percutaneous transhepatic gallbladder drainage of gallbladder empyema before laparoscopic cholecystectomy.this study is conducted to evaluate the feasibility of percutaneous transhepatic gallbladder drainage prior to laparoscopic cholecystectomy for the treatment of gallbladder empyema. we also determine the sonographic findings, causative organism, clinical signs and symptoms, laboratory data, associated underlying medical disorders and the complications related to both cholecystostomy and laparoscopic cholecystectomy.200011020851
homology-based molecular modelling of plp-dependent histidine decarboxylase from mmorganella morganii.the 3-d structural information is a prerequisite for a rational ligand design. in the absence of experimental data, model building on the basis of a known 3-d structure of a homologous protein is at present the only reliable method to obtain structural information. a homology model building study of the pyridoxal 5'-phosphate (plp)-dependent histidine decarboxylase from morganella morganii (hdc-mm) has been carried out based on the crystal structure of the aspartate aminotransferase from escheri ...200010906409
resolution of high-molecular-weight components in lipopolysaccharides of escherichia coli, morganella morganii, citrobacter freundii and citrobacter diversus strains with sodium dodecyl sulfate polyacrylamide gels.the use of 0.5% sodium dodecyl sulfate in polyacrylamide separation gels allowed the resolution in several bands of high-molecular-mass components in smooth lipopolysaccharide of bacterial outer membrane from escherichia coli, morganella morganii, citrobacter freundii and citrobacter diversus. with or without 0.1% sds, however, such a result was not possible.200010576704
a novel integron in salmonella enterica serovar enteritidis, carrying the bla(dha-1) gene and its regulator gene ampr, originated from morganella morganii.the genetic organization of the gene coding for dha-1 and the corresponding ampr gene was determined by pcr mapping. these genes have been mobilized from the morganella morganii chromosome and inserted into a complex suli-type integron, similar to in6 and in7. however, they are not themselves mobile cassettes. this integron probably includes a specific site for recombination allowing the mobilization of diverse resistance genes, as observed for bla(cmy-1) and bla(mox-1).200010602756
cost-effective and rapid presumptive identification of gram-negative bacilli in routine urine, pus, and stool cultures: evaluation of the use of chromagar orientation medium in conjunction with simple biochemical tests.the algorithm for a new identification system was designed on the basis of colony color and morphology on chromagar orientation medium in conjunction with simple biochemical tests such as indole (ind), lysine decarboxylase (ldc), and ornithine decarboxylase (odc) utilization tests with gram-negative bacilli isolated from urine samples as well as pus, stool, and other clinical specimens by the following colony characteristics, biochemical reactions, and serological results: pinkish to red, ind po ...200011101600
beta-lactam susceptibility patterns and investigation of cephalosporin hydrolysing beta-lactamases of gram-negative extraintestinal clinical isolates.of more than 3500 isolates of enterobacteriaceae, 48-69% were resistant to aminopenicillins and 11-45% to amoxycillin+clavulanic acid. resistance to second and third generation cephalosporins was present in 11-17 and 3-8% of escherichia coli, 47-56 and 15-52% of klebsiella-enterobacter, 36-57 and 16-27% of proteus, providencia and morganella isolates. pseudomonas aeruginosa strains varied in their resistance to antipseudomonal beta-lactams. isoelectric points, inhibitor profiles and substrate pr ...200011118847
influence of intestinal flora on the development of fibrosis and cirrhosis in a rat model.the gut flora play a significant role in the disposition of many foreign substances, as well as producing nutrients and toxins that may be absorbed and reach the liver. this study examines the influence of antibiotic-induced alterations in gut flora on the development of hepatic fibrosis in a rat model.200011129226
fragmentation of 23s rrna in strains of proteus and providencia results from intervening sequences in the rrn (rrna) genes.intervening sequences (ivss) were originally identified in the rrl genes for 23s rrna (rrl genes, for large ribosomal subunit, part of rrn operon encoding rrna) of salmonella enterica serovars typhimurium lt2 and arizonae. these sequences are transcribed but later removed during rnase iii processing of the rrna, resulting in fragmentation of the 23s species; ivss are uncommon, but have been reported in at least 10 bacterial genera. through pcr amplification of ivs-containing regions of the rrl g ...200010648538
histamine and biogenic amine production by morganella morganii isolated from temperature-abused albacore.histamine-producing bacteria were isolated from albacore stored at 0, 25, 30, and 37 degrees c. they were screened using niven's differential medium, and their histamine production was confirmed by high-pressure liquid chromatography analysis. the optimum temperature for growth of histamine-producing bacteria was 25 degrees c. the bacterium producing the highest level of histamine was isolated from fish abused at 25 degrees c. it was identified as morganella morganii by morphological, cultural, ...200010678431
application of the bigelow (z-value) model and histamine detection to determine the time and temperature required to eliminate morganella morganii from seafood.in new zealand, the product most frequently implicated in cases of scombroid or histamine poisoning is the hot-smoked fish, kahawai (arripis trutta). a properly controlled heating step in the production of hot-smoked seafood could eliminate bacteria able to convert the amino acid histidine to histamine. in this study, we determined the core temperatures and times required during hot smoking of kahawai to eliminate histamine-forming bacteria and to ensure a final product that will not produce his ...200010678437
decreased permeability and enhanced proton-dependent active efflux in the development of resistance to quinolones in morganella morganii.five quinolone-resistant strains were developed from a clinical morganella morganii isolate (m1 strain) which was susceptible to nalidixic acid and fluoroquinolones. all five in vitro selected mutants showed diminished norfloxacin accumulation and two of them also decreased the expression of the ago in the outer membrane lipopolysaccharide with respect to their parent strain and to the m. morganii nctc-235 type strain. likewise, the m1 strain did not express a 37-38 kda protein and accumulated l ...200010720807
[two cases of purulent pericarditis as complication of myocardial infarction]. 200010723156
evaluation of the sirscan automated zone reader in a clinical microbiology laboratory.we compared readings of kirby-bauer plates by the sirscan, an automated image analyzer that measures zone diameters, to those of experienced clinical microbiologists measuring zones with a hand-held caliper interfaced to a computer and with a ruler. to read plates of escherichia coli, morganella morganii, and pseudomonas aeruginosa containing 12 antibiotic disks the sirscan took 11 s; technologists took 28 s by caliper and 39 s by ruler. reading times of four different technologists ranged from ...200010747172
in vitro antibacterial spectrum of a new broad-spectrum 8-methoxy fluoroquinolone, gatifloxacin.the in vitro antibacterial spectrum of gatifloxacin was compared with those of ciprofloxacin and ofloxacin. gatifloxacin was two- to four-fold more potent than comparator quinolones against staphylococci, streptococci, pneumococci and enterococci (gatifloxacin mic90s, < or =1 mg/l, except 4 mg/l against methicillin-resistant staphylococcus aureus and enterococcus faecium). gatifloxacin was two-fold less potent than ciprofloxacin, and the same as or two-fold more potent than ofloxacin against ent ...200010747819
type i nitroreductases in soil enterobacteria reduce tnt (2,4,6,-trinitrotoluene) and rdx (hexahydro-1,3,5-trinitro-1,3,5-triazine).many enteric bacteria express a type i oxygen-insensitive nitroreductase, which reduces nitro groups on many different nitroaromatic compounds under aerobic conditions. enzymatic reduction of nitramines was also documented in enteric bacteria under anaerobic conditions. this study indicates that nitramine reduction in enteric bacteria is carried out by the type i, or oxygen-insensitive nitroreductase, rather than a type ii enzyme. the enteric bacterium morganella morganii strain b2 with document ...200010749541
in vitro activity of gemifloxacin against a broad range of recent clinical isolates from the usa.the antibacterial potencies of gemifloxacin (sb-265805) and 13 comparator compounds were determined by broth microdilution against a panel of 645 gram-positive and 995 gram-negative organisms collected from various usa sites. time-kill studies were performed and postantibiotic effect (pae) was determined for several organisms using trovafloxacin and ciprofloxacin as comparator compounds. based on mic(90)s, gemifloxacin was the most potent compound tested against gram-positive isolates: streptoco ...200010824027
phosphorylation of nucleosides by the mutated acid phosphatase from morganella morganii.a novel nucleoside phosphorylation process using the food additive pyrophosphate as the phosphate source was investigated. the morganella morganii gene encoding a selective nucleoside pyrophosphate phosphotransferase was cloned. it was identical to the m. morganii phoc acid phosphatase gene. sequential in vitro random mutagenesis was performed on the gene by error-prone pcr to construct a mutant library. the mutant library was introduced into escherichia coli, and the transformants were screened ...200010877772
tem-72, a new extended-spectrum beta-lactamase detected in proteus mirabilis and morganella morganii in italy.a new natural tem-2 derivative, named tem-72, was identified in a proteus mirabilis strain and in a morganella morganii strain isolated in italy in 1999. compared to tem-1, tem-72 contains the following amino acid substitutions: q39k, m182t, g238s, and e240k. kinetic analysis showed that tem-72 exhibits an extended-spectrum activity, including activity against oxyimino-cephalosporins and aztreonam. expression of bla(tem-72) in escherichia coli was capable of decreasing the host susceptibility to ...200010952610
classification, identification, and clinical significance of proteus, providencia, and morganella.this review presents the current taxonomy of the genera proteus, providencia, and morganella, along with the current methods for the identification of each species within the three genera, incorporating both conventional biochemical and commercial methods. while all of these organisms are ubiquitous in the environment, individual case reports and nosocomial outbreak reports that demonstrate their ability to cause major infectious disease problems are presented. lastly, anticipated antimicrobial ...200011023955
classification of proteus vulgaris biogroup 3 with recognition of proteus hauseri sp. nov., nom. rev. and unnamed proteus genomospecies 4, 5 and 6.strains traditionally identified as proteus vulgaris formed three biogroups. biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as proteus penneri. biogroup 2, characterized by positive reactions for indole, salicin and aesculin, was shown by dna hybridization (hydroxyapatite method) to be a genetic species separate from biogroup 1 and from biogroup 3 which is positive for indole production and negative for salicin an ...200011034498
[verification of identification of infectious agents by culture in routine gynecologic cytological screening].the original bethesda classification system for reporting cervical/vaginal cytologic diagnoses has claimed besides oncologic evaluation also a statement on the presence of infectious agents. their diagnosis should be followed by appropriate treatment. based upon the comparison of careful bacterioscopic study in a series of 175 routine cervical smears with the results of microbiological, virological and mycological examinations the following pathogens might be-according to the authors' opinion-di ...199911038666
selective medium for primary isolation of members of the tribe proteeae.a selective proteeae medium (spm) for isolation and preliminary detection of species of genera proteus, morganella, and providencia was evaluated. the spm contains tryptose phosphate agar with phenolphthalein monophosphate (as substrate for phosphatase activity), bile salts and polymyxin b (as inhibitors). the selectivity of the spm was tested by the ecometric method of quality assurance of culture media. fourteen reference cultures of enterobacteria and fifty-four strains of proteeae were teste ...199911097023
fatal early onset infection in an extremely low birth weight infant due to morganella morganii.this paper reports a case of chorioamnionitis due to morganella morganii in a mother who presented with ruptured membranes at 24 weeks' gestation and was treated with dexamethasone and prophylactic ampicillin. her premature infant developed severe early onset infection due to the same organism and expired.199910685307
comparative evaluation of five chromogenic media for detection, enumeration and identification of urinary tract pathogens.five chromogenic agar plates--cps id2 medium (biomérieux, france), chromagar orientation medium (becton dickinson, france), uriselect3 medium (sanofi diagnostics pasteur, france), rainbow agar uti medium (biolog, usa) and chromogenic uti medium (oxoid, germany)--for the detection, enumeration and direct identification of urinary tract pathogens were compared using 443 urine specimens at two hospital laboratories. the enumeration of microorganisms was consistent on the five media for 403 of the 4 ...199910614954
[the results of a multicenter study of the comparative activity of cefepime and other antibiotics against the causative agents of severe hospital infections (the micromax program)].a multicentre trial was performed on the activity of cefepime in comparison with ceftazidime, ceftriaxone, piperacillin/tazobactam, imipenem and ciprofloxacin against severe hospital infection pathogens in intensive care units. the isolates of escherichia coli and proteus spp. from the majority of the centres were highly susceptible to the antibiotics (90 to 100 per cent of the isolates). in some centres up to 40 per cent of the isolates produced esbl. the isolates of klebsiella spp. were charac ...199910629736
molecular recognition of synthetic siderophore analogues: a study with receptor-deficient and fhu(a-b) deletion mutants of escherichia coli.the biological activity of six synthetic siderophore analogues (two dihydroxamates, two trihydroxamates, one tetrahydroxamate and one 3-hydroxy-4(1h)pyridinone) has been studied in escherichia coli, morganella morganii 13 and proteus mirabilis 8993 strains by using growth promotion tests. various transport-deficient mutants of e. coli were used to study the route of entry into gram-negative bacteria. the results indicated that the synthetic hydroxamate compounds are transported via fhu-mediated ...199910581683
cefotaxime-hydrolysing beta lactamases in morganella morganii.the frequency of enterobacterial isolates with high resistance to expanded-spectrum beta-lactam antibiotics (mainly cefotaxime or ceftriaxone) has increased notoriously in argentina, mainly because of the spread of extended-spectrum beta-lactamases. the aim of this work was the study of extended-spectrum beta-lactamases in several morganella morganii isolates with unusually high resistance to ceftriaxone. these strains produced at least two beta-lactamases, of apparent pis of 5.4 and 8.2, molecu ...199910584905
molecular characterization of a ceftazidime-resistant morganella morganii isolate producing a tem-10 beta-lactamase. 19999989337
bacteremia caused by digestive system endoscopy.to evaluate bacteremias caused during endoscopic examination of the digestive tract.199910231302
in vivo activities of peptidic prodrugs of novel aminomethyl tetrahydrofuranyl-1 beta-methylcarbapenems.a series of novel aminomethyl tetrahydrofuranyl (thf)-1 beta-methylcarbapenems which have excellent broad-spectrum antibacterial activities exhibit modest efficacies against acute lethal infections (3.8 mg/kg of body weight against escherichia coli and 0.9 mg/kg against staphylococcus aureus) in mice when they are administered orally. in an effort to improve the efficacies of orally administered drugs through enhanced absorption by making use of a peptide-mediated transport system, several diffe ...199910049251
segmented filamentous bacteria are potent stimuli of a physiologically normal state of the murine gut mucosal immune system.segmented filamentous bacteria (sfb) are autochthonous bacteria inhabiting the intestinal tracts of many species, including humans. we studied the effect of sfb on the mucosal immune system by monoassociating formerly germfree c3h/hen mice with sfb. at various time points during 190 days of colonization, fragment cultures of small intestine and peyer's patches (pp) were analyzed for total immunoglobulin a (iga) and sfb-specific iga production. also, phenotypic changes indicating germinal center ...199910085047
cloning, sequence analyses, expression, and distribution of ampc-ampr from morganella morganii clinical isolates.shotgun cloning experiments with restriction enzyme-digested genomic dna from morganella morganii 1, which expresses high levels of cephalosporinase, into the pbkcmv cloning vector gave a recombinant plasmid, ppon-1, which encoded four entire genes: ampc, ampr, an hybf family gene, and orf-1 of unknown function. the deduced ampc beta-lactamase of pi 7.6 shared structural and functional homologies with ampc from citrobacter freundii, escherichia coli, yersinia enterocolitica, enterobacter cloacae ...199910103179
[the use of minipigs to produce diagnostic sera for the serological identification of bacteria in the genus morganella].mini-pigs of the svetlogorsk breed were immunized with vaccine prepared from of m. morganii culture; the culture had been grown in a liquid nutrient medium, obtained from casein hydrolysate, in flasks placed in a thermostatically controlled shaker to the phases of exponential growth and cell growth deceleration. o-agglutinating sera thus obtained were specific. the highest antibody level was detected in the serum obtained as the result of the immunization of guinea pigs with the vaccine prepared ...199910356725
resistance to third-generation cephalosporins in adult gram-negative bacillary meningitis.ninety-three patients with gram-negative bacillary meningitis (gnbm) were identified at kaohsiung chang gung memorial hospital, over a period of 12 years. among them, eight showed resistance to third-generation cephalosporins, accounting for 9% of the total gnbm cases and 29% of the postneurosurgical gnbm cases. the resistant pathogens included acinetobacter baumannii, klebsiella pneumoniae, citrobacter freundii and morganella morganii. these eight patients, six males and two females aged 18-61 ...199910378134
[out of hospital pneumonia by morganella morganii]. 199910396093
susceptibility to levofloxacin of clinical isolates of bacteria from intensive care and haematology/oncology patients in switzerland: a multicentre study.the objective of this study was to examine the susceptibility of clinical isolates to levofloxacin, a fluoroquinolone with extended activity against gram-positive bacteria, and other antibiotics in 12 swiss clinical microbiology laboratories using the nccls disc diffusion technique. isolates were prospectively collected from intensive care units (icus (59%), oncology wards (7%) and other units with haematology/oncology patients (34%) from june 1995 to march 1996. the levofloxacin breakpoints use ...199910404338
prevalence of proteus species in urinary tract infections in a regional hospital in trinidad.proteus bacteria are a well-known cause of urinary tract infections (utis). the prevalence of utis is high among catheterized patients and those undergoing manipulation of the urinary tract. this study assessed the prevalence of utis due to proteus species, the predisposing factors, complications and extent of antimicrobial resistance at a regional teaching hospital.199910418178
in vitro and in vivo activities of syn2190, a novel beta-lactamase inhibitor.syn2190, a monobactam derivative containing 1,5-dihydroxy-4-pyridone as the c-3 side chain, is a potent inhibitor of group 1 beta-lactamase. the concentrations of inhibitor needed to reduce the initial rate of hydrolysis of substrate by 50% for syn2190 against these enzymes were in the range of 0.002 to 0.01 microm. these values were 220- to 850-fold lower than those of tazobactam. syn2190 showed in vitro synergy with ceftazidime and cefpirome. this synergy was dependent on the concentration of ...199910428909
antimicrobial activity of elaeocarpus sphaericus.antibacterial activity of petroleum ether (pe), benzene (be), chloroform (ce), acetone (ae) and ethanol (ee) extracts of dried elaeocarpus sphaericus fruit was investigated against 28 gram-positive and gram-negative bacteria using the disc diffusion and plate dilution methods. the ae fraction showed marked antimicrobial activity against ten organisms. be was active against salmonella typhimurium and morganella morganii, and ee against plesiomonas shigelloides, shigella flexnerii and sh. sonneii. ...199910441793
molecular characterization of the genera proteus, morganella, and providencia by ribotyping.the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ...199910449462
in vitro activity of newer broad spectrum beta-lactam antibiotics against enterobacteriaceae and non-fermenters: a report from austrian intensive care units. austrian carbapenem susceptibility surveillance group.we compared the in vitro activity of broad spectrum beta-lactam antibiotics against 573 gram-negative isolates (enterobacteriaceae and non-fermenters) collected between november 1996 and may 1997 from 9 laboratories serving intensive care units throughout austria. mic's (minimal inhibitory concentration) were obtained with the e-test for meropenem, imipenem, ceftazidime, cefepime, cefpirome and piperacillin/tazobactam. pseudomonas aeruginosa was the most frequently isolated organism (22%), follo ...199910467641
biogenic amine survey and organoleptic changes in fresh, stored, and temperature-abused bluefish (pomatomus saltatrix).changes in histamine, putrescine, and cadaverine concentrations in bluefish filets (pomatomus saltatrix) stored at 5, 10, and 15 degrees c were determined using high-performance liquid chromatography. an organoleptic assessment was conducted simultaneously with the biogenic amine analyses. the histamine levels found in fresh bluefish obtained from wholesale seafood distributors ranged between <1 ppm and 99 with an average of 39 ppm. putrescine and cadaverine were not found in fresh bluefish. fis ...199910492478
phylogenetic analysis of l4-mediated autogenous control of the s10 ribosomal protein operon.we investigated the regulation of the s10 ribosomal protein (r-protein) operon among members of the gamma subdivision of the proteobacteria, which includes escherichia coli. in e. coli, this 11-gene operon is autogenously controlled by r-protein l4. this regulation requires specific determinants within the untranslated leader of the mrna. secondary structure analysis of the s10 leaders of five enterobacteria (salmonella typhimurium, citrobacter freundii, yersinia enterocolitica, serratia marcesc ...199910498727
antibiotic sensitivity of bacteria associated with community-acquired urinary tract infection in britain.twelve laboratories in different parts of britain each supplied approximately 80 consecutive urinary bacterial isolates from community patients. all strains were identified by a central laboratory, where sensitivity to a variety of orally administered antimicrobials was determined by microtitre broth dilution. 65.1% of isolates were escherichia coli, 23.4% 'coliforms' other than e. coli, 4.6% proteus and morganella spp., 1.8% pseudomonas spp., 2.4% enterococci, 0.7% group b streptococci, 1.5% co ...199910511403
in vitro antibacterial activity of fk041, a new orally active cephalosporin.the in vitro activity of fk041, a new orally active cephem antibiotic, against a wide variety of clinical isolates of bacteria was investigated and compared with those of cefdinir (cfdn) and cefditoren (cdtr). fk041 exhibited broad spectrum activity against reference strains of gram-positive and gram-negative aerobes and anaerobes. fk041 was active against clinical isolates of gram-positive organisms except enterococcus faecalis with mic90s less than 1.56 microg/ml. fk041 was more active than cf ...199910513845
the use of chromagar orientation as a primary isolation medium with presumptive identification for the routine screening of urine specimens.the aim of the study was to compare the use of a novel differential culture medium chromagar, for both primary isolation and presumptive identificaton, with the method currently used in our laboratory for screening mid-stream-urine samples (msu). routine methods (rm) included blotting paper imprinting of all specimens and additional quantitative culture on cysteine lactose electrolyte-deficient agar (cled) for selected samples together with microbact 12e for further identification. the chromagar ...199910519322
regulation of inducible ampc beta-lactamase expression among enterobacteriaceae.ampc ss-lactamases are active-site serine enzymes that are primarily cephalosporinases. in many gram negative organisms, including enterobacter spp.,citrobacter freundii, serratia marcescens, morganella morganii and pseudomonas aeruginosa, the expression of chromosomal ampc genes is low but inducible in response to ss-lactams and other stimuli. the current working model for ampc induction requires exposure of bacterial cells to ss-lactam drugs or other stimuli and is linked to the cell wall recy ...199910539994
in vitro antibacterial activity of men 10700, a new penem antibiotic.we report here the antibacterial activity of the new penem antibiotic men 10700 against a total of 740 gram-positive and gram-negative clinical isolates, in comparison to imipenem, meropenem, cefotaxime, ceftriaxone, ciprofloxacin and gentamicin. men 10700 has shown a wide spectrum of antibacterial activity, with a potent activity both against gram-positive species (with the exception of methicillin-resistant staphylococcus aureus and staphylococcus epidermidis) as indicated by minimal inhibitor ...199910567770
isolation, cloning, and expression of an acid phosphatase containing phosphotyrosyl phosphatase activity from prevotella intermedia.a novel acid phosphatase containing phosphotyrosyl phosphatase (ptpase) activity, designated piacp, from prevotella intermedia atcc 25611, an anaerobe implicated in progressive periodontal disease, has been purified and characterized. piacp, a monomer with an apparent molecular mass of 30 kda, did not require divalent metal cations for activity and was sensitive to orthovanadate but highly resistant to okadaic acid. the enzyme exhibited substantial activity against tyrosine phosphate-containing ...199910559178
a novel selective nucleoside phosphorylating enzyme from morganella morganii.a selective nucleoside phosphorylating enzyme was purified to homogeneity from morganella morganii ncimb10466 crude extract. the enzyme appeared to consist of six subunits identical in molecular mass (m(r) 25,000). it phosphorylated various nucleosides at the 5'-position to produce nucleoside-5'-monophosphates using pyrophosphate as the phosphate source. energy-rich compounds, such as carbamylphosphate and acetylphosphate, were also very effective phosphate donors. the enzyme also exhibited phos ...199916232546
a family of stability determinants in pathogenic bacteria.a novel segregational stability system was identified on plasmid r485, which originates from morganella morganii. the system is composed of two overlapping genes, stbd and stbe, which potentially encode proteins of 83 and 93 amino acids, respectively. homologs of the stbde genes were identified on the enterotoxigenic plasmid p307 from escherichia coli and on the chromosomes of vibrio cholerae and haemophilus influenzae biogroup aegyptius. the former two homologs also promote plasmid stability in ...19989829958
bacterial acid phosphatase gene fusions useful as targets for cloning-dependent insertional inactivation.the morganella morganii phoc gene, encoding a class a acid phosphatase, was used to generate gene fusions with modified amino-terminal moieties of the escherichia coli lacz gene carrying a multiple-cloning site flanked by phage-specific promoters and recognition sites for universal sequencing primers. the corresponding hybrid proteins retained a phoc-like enzymatic activity which is easily detectable by a plate histochemical assay, rendering similar gene fusions potentially useful as targets for ...19989548775
identification and natural antibiotic susceptibility of morganella morganii.our data should elucidate whether or not natural antibiotic susceptibility can be used as an aid for subspecies or biovar discrimination of morganella morganii (ii). furthermore, our goal was to create a database of the natural susceptibility of m. morganii (iii) and we were interested in the relative frequency of the recently described subspecific taxa (i). on the basis of trehalose fermentation (tre), ornithine decarboxylase (odc), and lysine decarboxylase (ldc) activities, we determined the b ...19989572021
case of chorioamnionitis in an immunocompetent woman caused by morganella morganii.morganella morganii is an opportunistic organism that leads to serious morbidity. it is an enteric organism commonly associated with immunocompromise and chronic urinary catheterization. we present a case of a healthy gravid individual who presented with chorioamnionitis and neonatal seizures associated with m. morganii. this organism, which causes severe morbidity, is fortunately rare in obstetrics and gynecology.19989502663
unusual case of postoperative infection caused by morganella morganii.morganella morganii is a facultative gram-negative anaerobe present in the human gastrointestinal system as normal flora. clinically, the organism is important when it manifests as an opportunistic pathogenic infection elsewhere in the body. this paper reviews an unusual case in which m. morganii is the pathogen responsible for a devastating postoperative infection in a diabetic patient. currently, there are no known reported cases in the literature of m. morganii as an etiologic pathogen for a ...19989571462
interactions of beta-lactamases with sanfetrinem (gv 104326) compared to those with imipenem and with oral beta-lactams.sanfetrinem is a trinem beta-lactam which can be administered orally as a hexatil ester. we examined whether its beta-lactamase interactions resembled those of the available carbapenems, i.e., stable to ampc and extended-spectrum beta-lactamases but labile to class b and functional group 2f enzymes. the comparator drugs were imipenem, oral cephalosporins, and amoxicillin. mics were determined for beta-lactamase expression variants, and hydrolysis was examined directly with representative enzymes ...19989593145
imipenem resistance in aerobic gram-negative bacteria.a prospective study was undertaken to observe the emergence of resistance to imipenem, if any, among aerobic gram-negative bacteria. a total of 736 isolates were tested during 1994-95 and less than 1% of them were resistant to imipenem, whereas the next year ('95-'96) the rate increased to 11 of the 903 isolates tested. the resistant isolates during '94-'95 were all stenotrophomonas maltophilia whereas the spectrum of resistant bacterial species increased in '95-'96 to include pseudomonas aerugi ...19989603633
important and emerging beta-lactamase-mediated resistances in hospital-based pathogens: the amp c enzymes.resistance to third-generation cephalosporins mediated by beta-lactamases is an increasing problem for clinical therapeutics. a wide range of enterobacteriaceae produce these ampc enzymes (bush-jacoby-medeiros group 1), including enterobacter spp., citrobacter freundii, morganella morganii, providencia spp., and serratia marcescens. resistance via this mechanism has been shown to be statistically correlated with the use of some third-generation cephalosporins, and the infections caused by these ...19989635237
molecular characterization of a tem-21 beta-lactamase in a clinical isolate of morganella morganii.a clinical isolate of morganella morganii, with reduced susceptibility to expanded-spectrum cephalosporins and aztreonam, was found to produce an extended-spectrum beta-lactamase with a pi of 6.4. the nucleotide sequence of the encoding gene was that of the gene encoding tem-21. this is the first molecular characterization of an extended-spectrum beta-lactamase in m. morganii.19989687421
[pharmacokinetic, bacteriological and clinical evaluation of tazobactam/piperacillin in pediatrics].pharmacokinetic, bacteriological and clinical studies were performed in pediatrics on tazobactam/piperacillin (taz/pipc), a combined drug of a new beta-lactamase inhibitor tazobactam and piperacillin at a ratio of 1:4. 1. serum levels and urinary excretions of taz, pipc and desethyl piperacillin (det-pipc), a metabolite of pipc, after one shot intravenous administration of 50 mg/kg of taz/pipc to two children (6-7 years old) were investigated. the serum taz level at 0.08 hour was 50.8-51.0 micro ...19989693967
bacterial isolates from california sea lions (zalophus californianus), harbor seals (phoca vitulina), and northern elephant seals (mirounga angustirostris) admitted to a rehabilitation center along the central california coast, 1994-1995.in 2 yr of bacteriologic culturing of 297 california sea lions (zalophus californianus), 154 harbor seals (phoca vitulina), and 89 northern elephant seals (mirounga angustirostris) stranded alive along the california coast, the most frequent isolates from inflammatory lesions in live animals were escherichia coli, streptococcus viridans, listeria ivanovii, beta-hemolytic streptococcus spp., and enterococcus spp. this is the first report of l. ivanovii isolation from a marine mammal. the common i ...19989732032
salmonella enteritidis: ampc plasmid-mediated inducible beta-lactamase (dha-1) with an ampr gene from morganella morganii.dha-1, a plasmid-mediated cephalosporinase from a single clinical salmonella enteritidis isolate, conferred resistance to oxyimino-cephalosporins (cefotaxime and ceftazidime) and cephamycins (cefoxitin and moxalactam), and this resistance was transferable to escherichia coli hb101. an antagonism was observed between cefoxitin and aztreonam by the diffusion method. transformation of the transconjugant e. coli strain with plasmid pnh5 carrying the ampd gene (whose product decreases the level of ex ...19989736562
[a female case of fournier's gangrene in a patient with lupus nephritis].infection is one of the common causes of death in patients with systemic lupus erythematosus (sle). it is associated with the use of immunosuppressive agents, renal failure, and increased disease activity. fournier's gangrene is a necrotizing fasciitis occurring in the genital region. it is rare, but can be crucial if surgical drainage is delayed. we report a female case of fournier's gangrene occurring in a patient with lupus nephritis and chronic renal failure. the patient was a 21-year-old fe ...19989752693
[antibiotic sensitivity to isolated bacteria in pediatric urinary tract infections].of the childhood urinary tract infections, more than 50% are caused by escherichia coli (e coli), followed by proteus mirabilis (p mirabilis), klebsiella sp, other enterobacteria, enterococci, pseudomonas aeruginosa, and staphylococci. of e coli isolates, 50 to 60% are resistant to ampicillin (ampi-r), 10% being susceptible to amoxicillin + clavulanic acid (amc). for p mirabilis, ampi-r isolates are less frequent and more often susceptible to amc. klebsiella sp is resistant to ampicillin, 75% of ...19989759315
[bactericidal activity and capacity for selection of mutants resistant to imipenmen and meropenem in strains of proteus, morganella and providencia].to compare the bactericidal activity and frequency of mutation for meropenem and imipenem against proteus mirabilis, morganella morganii and providencia rettgeri.19989763741
gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria.naturally produced membrane vesicles (mvs), isolated from 15 strains of gram-negative bacteria (citrobacter, enterobacter, escherichia, klebsiella, morganella, proteus, salmonella, and shigella strains), lysed many gram-positive (including mycobacterium) and gram-negative cultures. peptidoglycan zymograms suggested that mvs contained peptidoglycan hydrolases, and electron microscopy revealed that the murein sacculi were digested, confirming a previous modus operandi (j. l. kadurugamuwa and t. j. ...19989765585
ecthyma gangrenosum-like eruption associated with morganella morganii infection.ecthyma gangrenosum is considered as a pathognomonic sign of pseudomonas aeruginosa sepsis. lesions similar to ecthyma gangrenosum may be caused by other organisms. we report a case of an ecthyma gangrenosum-like eruption caused by morganella morganii, a gram-negative bacillus.19989767304
[a national multicenter study of the in-vitro activity of piperacillin-tazobactam. the spanish piperacillin-tazobactam group].the action of piperacillin-tazobactam on 4,137 of the 4,364 strains collected in a spanish multicenter study involving 46 participating hospitals was studied. the samples were from the following: 41% urine, 18% exuded from wound abscesses, 13% respiratory, 9% blood, 3% peritoneal liquid, and the remainder from other various sources. the gram-negative bacteria included 2,778 strains from 13 genera: escherichia (1,289), pseudomonas (451), proteus (230), klebsiella (203), haemophilus (172), enterob ...19989795299
[physiologic-biochemical features of morganella under various conditions of cultivation].the parameters of the cultivation process and the content of protein and polysaccharide in m.morganii cells grown under the conditions of submerged cultivation in 2 nutrient media having different composition were studied. the study revealed that the highest values of specific growth rate and the maximum yield of biomass were observed during cultivation under the conditions of medium values of oxygen concentration in the medium (po2 = 20-30%). the content of protein in bacterial cells was practi ...19989825487
[comparative study of morganella and providencia bacteriophages].7 strains of m.morganii phages and 7 strains of p.rettgeri phages were isolated from lysogenic cultures and the environment. the main biological properties of these phages were studied. the phages under study formed independent taxonomic groups. these phages were found to be highly specific and capable of being used for the identification of bacteria.19989825491
studies on the formation of crystalline bacterial biofilms on urethral catheters.a model of the catheterised bladder was used to test the ability of urease-producing urinary tract pathogens to encrust urethral catheters. encrustation was assessed by determining the amounts of calcium and magnesium deposited on the catheters and visualised by scanning electron microscopy. urease-positive morganella morganii, klebsiella pneumoniae, and pseudomonas aeruginosa failed to raise the urinary ph and form crystalline biofilms. in contrast, strains of proteus mirabilis, proteus vulgari ...19989832268
[in vitro antimicrobial activity of cefpirome compared to other broad-spectrum beta-lactam drugs against 804 clinical isolates from 9 brazilian hospitals].to evaluate the in vitro activity of the fourth-generation cephalosporin cefpirome in comparison to that of ceftazidime, ceftriaxone, cefotaxime and imipenem in a multicenter study involving nine hospitals from six cities (four states).19989852647
microbiological examinations and in-vitro testing of different antibiotics in therapeutic endoscopy of the biliary system.prior to endoscopic therapeutic procedures, no antibiotic prophylaxis is administered routinely. because of the reported incidence of infectious complications, which may reach up to 10%, a prospective study was undertaken to investigate the effects of a prophylactic dose of cefuroxime on the incidence of bacteremia and clinical signs of infection, but no significant effects could be demonstrated. in addition to this published work, blood and bile cultures obtained in this trial were also investi ...19989865561
utility of serial rectal swab cultures for detection of ceftazidime- and imipenem-resistant gram-negative bacilli from patients in the intensive care unit.forty-four patients receiving intensive care were studied prospectively to assess the utility of serial rectal swab cultures and clinical correlates of resistance for pseudomonas aeruginosa, enterobacter spp., citrobacter spp., morganella morganii, and serratia marcescens strains resistant to ceftazidime or imipenem. strains of pseudomonas aeruginosa, enterobacter spp., citrobacter spp., or morganella morganii were found in 26 of 44 (59%) patients: 17 (65%) in clinical sites (11 with concomitant ...19989865988
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