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identification of lytic bacteriophage mmp1, assigned to a new member of t7-like phages infecting morganella morganii.mmp1 (morganella morganii phage 1) is a lytic bacteriophage newly isolated from the host bacterium m. morganii. the entire genome was sequenced, and final assembly yielded a 38,234bp linear double-stranded dna (dsdna) with a g+c content of 46.5%. in the mmp1 genome, 49 putative genes, 10 putative promoters and 2 predicted sigma-independent terminators were determined through bioinformatic analysis. a striking feature of the mmp1 genome is its high degree of similarity to the t7 group of phages. ...201020561579
[unusual cause of infant severe community-acquired pneumonia: morganella morganii]. 201020570476
phylogenetic analysis of the genera proteus, morganella and providencia by comparison of rpob sequences of type and clinical strains suggests the classification of proteus myxofaciens in a new genus cosenzaea gen. nov., as cosenzaea myxofaciens comb. nov.phylogenetic analysis of partial rpob gene sequences of type and clinical strains belonging to different 16s rrna gene-fingerprinting ribogroups within 11 species of enterobacteria of the genera proteus, morganella, and providencia allowed to define rpob clades supported by high bootstrap values and confirmed by >/=2.5% nucleotide divergence. no rpob clade included strains belonging to different species and the majority of the species were confirmed as discrete and homogeneous, since they consti ...201020709916
seasonal variation, chemical composition, and analgesic and antimicrobial activities of the essential oil from leaves of tetradenia riparia (hochst.) codd in southern brazil.the seasonal variation of the chemical composition of the essential oil from fresh leaves of tetradenia riparia (hochst.) codd grown in southern brazil was analyzed by gc-ms, and the analgesic and antimicrobial activities of this oil were assayed. the yield of essential oil ranged from 0.17% to 0.26%, with the maximum amount in winter and the minimum in spring. the results obtained from principal components analysis (pca) revealed the existence of high chemical variability in the different seaso ...201020714310
morganella morganii pericarditis in a child with x-linked agammaglobulinemia. 201020723125
prospective study of urinary tract infection surveillance after kidney transplantation.urinary tract infection (uti) remains one of the main complications after kidney transplantation and it has serious consequences.201020723254
screening of the constituents, antimicrobial and antioxidant activity of endemic origanum hypericifolium o. schwartz & p.h. davis.the chemical compositions, total phenol content, antioxidant and antimicrobial activities with oxidant status of the essential oil from an endemic turkish species, origanum hypericifolium, were investigated. steam distillation (sd) was used to isolate the essential oils, and the chemical analyses were performed by gas chromatography-mass spectrometry (gc-ms). the antimicrobial activity was tested by agar disc diffusion method against morganella morganii (clinic isolate), micrococcus flavus (clin ...201020835958
new delhi metallo-beta lactamase (ndm-1) in enterobacteriaceae: treatment options with carbapenems compromised.carbapenems are among the few useful antibiotics against multidrug resistant gram negative bacteria particularly those with extended spectrum beta lactamase. however resistance to carbapenems occurs and is mediated by mechanisms like loss of outer membrane proteins and production of beta lactamase that is capable of hydrolyzing carbapenems. an alert issued in the uk in 2009 warned of an increasing number of carbapenem resistant enterobacteriaceae strains identified in uk hospital patients. many ...201020848811
detection and pfge analysis of esbl-producing isolates of proteus species isolated from patients at tehran hospitals.the production of extended-spectrum β-lactamases (esbls), in particular tem and ctx-m type, by the strains of proteus spp. is a contributing factor to the emergence of bacterial drug resistance. the aim of this study was to screen and determine of esbls genes among strains of proteus spp. at tehran hospitals.201020885345
diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania.aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ...201020941581
[viable non-culturable bacteria].viable but non-culturable cells (vbnc) are defined as live bacteria, but which do not either grow or divide. such bacteria cannot be cultivated on conventional media (they do not form colonies on solid media, they do not change broth appearance), but their existence can be proved using other methods. the switch to the vbnc stage has been described and documented for several bacterial species: vibrio spp. (cholerae, vulnificus and other species), escherichia coli (including ehec), campylobacter j ...201021038700
comparison of bacterial diversity in wheat bran and in the gut of larvae and newly emerged adult of musca domestica (diptera: muscidae) by use of ethidium monoazide reveals bacterial colonization.the objective of the current study is to investigate the bacterial colonization within the gut of the house fly, musca domestica l. (diptera: muscidae), at the larval stage and the bacterial community of the gut of the house fly at the newly emerged adult stage. after using ethidium monoazide to inhibit recovery of nucleic acids from dead bacteria, three polymerase chain reaction (pcr)-amplified 16s rdna libraries from wheat bran, larvae, and newly emerged adults was constructed, analyzed, and c ...201021061987
predation of human pathogens by the predatory bacteria micavibrio aeruginosavorus and bdellovibrio bacteriovorus.the focus of this study was to evaluate the potential use of the predatory bacteria bdellovibrio bacteriovorus and micavibrio aeruginosavorus to control the pathogens associated with human infection.201021114596
laboratory detection of extended-spectrum-beta-lactamase-producing enterobacteriaceae: evaluation of two screening agar plates and two confirmation techniques.the worldwide prevalence of extended-spectrum-beta-lactamase-producing esbl-producing enterobacteriaceae (esbl-e) is increasing, making the need for optimized detection techniques more urgent. in this study we investigated the performance of two esbl-e screening and two esbl-e confirmation techniques. in accordance with the dutch national guidelines (www.wip.nl), a collection of 642 highly resistant enterobacteriaceae strains, as identified by vitek2, was used to test the performances of two scr ...201021123527
recurrent lower limb abscesses as the primary manifestation of aorto-femoral graft paraprosthetic fistula.a 62-year-old man was presented to our facility with recurrent right lower limb abscesses. he had an aortobifemoral graft for an aortoiliac occlusive disease. the diagnosis of paraprosthetic fistula was confirmed by performing a high-resolution contrast-enhanced computed tomography, whereas labeled leukocyte imaging provided a negative result. the graft present in the right limb was removed and extra-anatomical femoro-femoral bypass was performed along with segmental ileal resection of the affec ...201021126852
bacterial kinetics-controlled shape-directed biosynthesis of silver nanoplates using morganella psychrotolerans.we show for the first time that by controlling the growth kinetics of morganella psychrotolerans, a silver-resistant psychrophilic bacterium, the shape anisotropy of silver nanoparticles can be achieved. this is particularly important considering that there has been no report that demonstrates a control over shape of ag nanoparticles by controlling the growth kinetics of bacteria during biological synthesis. additionally, we have for the first time performed electrochemistry experiments on bacte ...201021142094
morganella morganii could be an important intensive care unit pathogen. 201021253351
structural characterization and mhcii-dependent immunological properties of the zwitterionic o-chain antigen of morganella morganii.morganella morganii is a commensal gram-negative bacterium that has long been known to produce an antigen bearing phosphocholine groups. we determined the structure of this o-chain antigen and found that its repeating unit also contains a free amino group and a second phosphate: this alternating charge character places the m. morganii o-chain polysaccharide into a small family of zwitterionic polysaccharides (zpss) known to induce t-cell-dependent immune responses via presentation by class ii ma ...201121321054
neonatal morganella morganii sepsis: a case report and review of the literature. 201121342342
polymicrobial necrotizing fasciitis involving enterobacteria producing ctx-m-15 extended-spectrum β-lactamases.necrotizing fasciitis due to multiple gram-negative organisms in a nigerian patient is described. morganella morganii and citrobacter freundii carrying the ctx-m-15 extended-spectrum β-lactamase gene were isolated, highlighting the emergence of this β-lactamase in western africa and its successful spread amongst a wider range of members of the enterobacteriaceae.201120813849
finding recurrent copy number alterations preserving within-sample homogeneity.copy number alterations (cnas) represent an important component of genetic variation and play a significant role in many human diseases. development of array comparative genomic hybridization (acgh) technology has made it possible to identify cnas. identification of recurrent cnas represents the first fundamental step to provide a list of genomic regions which form the basis for further biological investigations. the main problem in recurrent cnas discovery is related to the need to distinguish ...201121873327
tumor abscess formation caused by morganella morganii complicated with bacteremia in a patient with gastrointestinal stromal tumor.we report the case of a 22-year-old man who presented with a 3-day history of watery diarrhea, abdominal pain, and fever. an image of the abdomen showed a heterogeneously echogenic mass lesion in the pelvis. the results of the blood cultures performed on admission showed the presence of morganella morganii. computed tomography-guided tube drainage was performed, and a culture of the drained abscess fluid yielded m. morganii growth. exploratory laparotomy with segmental resection of the jejunum a ...201121924697
extended-spectrum beta-lactamase orthopedic wound infections in nigeria.extended-spectrum beta-lactamase (esbl)-producing gram-negative bacteria are emerging and impacting significantly on the management of patients and hospital costs. besides, they are not being routinely sought after in diagnostic laboratories thus contributing to treatment failure.201121887050
[extracellular slime production and adhesion of morganella morganii strains to polystyrene].the aim of this study was the evaluation of the ability of extracellular slime production and adhesive properties of m. morganii strains. this study included 50 of m. morganii strains isolated from clinical samples. all of these strains were isolated in the clinical microbiology department of dr. a. jurasz university hospital in 2008-2009. five (10.0%) out of 50. m. morganii strains demonstrated extracellular slime production. adherence to polystyrene revealed 36 (72.0%) of m. morganii strains i ...201122184894
surgery in management of snake envenomation in children.snakebite is common in children especially in the developing countries. this study was undertaken to determine the role of surgery in the treatment of venomous snake bite in pediatric patients.201121877258
Clinical validation of integrated nucleic acid and protein detection on an electrochemical biosensor array for urinary tract infection diagnosis.Urinary tract infection (UTI) is a common infection that poses a substantial healthcare burden, yet its definitive diagnosis can be challenging. There is a need for a rapid, sensitive and reliable analytical method that could allow early detection of UTI and reduce unnecessary antibiotics. Pathogen identification along with quantitative detection of lactoferrin, a measure of pyuria, may provide useful information towards the overall diagnosis of UTI. Here, we report an integrated biosensor platf ...201122066011
description and plasmid characterization of qnrd determinants in proteus mirabilis and morganella morganii.clin microbiol infect abstract: we investigated the presence of qnrc and qnrd among 756 non-replicate enterobacteriaceae isolated in italy, selected for being non-susceptible to fluoroquinolones and/or resistant to third-generation cephalosporins. four proteus mirabilis and one morganella morganii (0.66% of the total) presented a qnrd gene, located in a 2687-base-pair plasmid that was entirely sequenced. the plasmid is un-typable, and contains no known coding region other than qnrd. that the qnr ...201122192340
Characterisation of histamine-producing bacteria from farmed blackspot seabream (Pagellus bogaraveo) and turbot (Psetta maxima).Turbot (Psetta maxima) and blackspot seabream (Pagellus bogaraveo) represent two of the most important emerging farmed fish species in European countries. However, no information of the presence and development of histamine-producing bacteria on them has been reported so far. Accordingly, the aim of this study was to isolate and identify the main histamine-producing bacteria in farmed turbot and blackspot seabream. For this study, 24 isolates (12 from turbot and 12 from blackspot seabream) were ...201121925757
dissemination of the rmtb gene carried on incf and incn plasmids among enterobacteriaceae in a pig farm and its environment.to investigate the prevalence and characterization of 16s rrna methylase-producing bacteria in a pig farm and its environment in east china.201121852287
Effects of wilting and molasses addition on fermentation and bacterial community in guinea grass silage.Aims: Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. Methods and Results: Direct-cut and wilted guinea grass silage was prepared with and without molasses. A high acetic acid level was observed during the fermentation of direct-cut silage, and long storage inc ...201122150390
phylogenetic characterization of bacteria in the gut of house flies (musca domestica l.).house flies (musca domestica l.) are cosmopolitan, ubiquitous, synanthropic insects that serve as mechanical or biological vectors for various microorganisms. to fully assess the role of house flies in the epidemiology of human diseases, it is essential to understand the diversity of microbiota harbored by natural fly populations. this study aimed to identify the diversity of house fly gut bacteria by both culture-dependent and culture-independent approaches. a total of 102 bacterial strains wer ...201122092755
antibacterial effect of food additives and detergents against histamine-producing bacteria on food contact material surfaces.we investigated the antibacterial activity of food additives and detergents against histamine-producing bacteria on food contact material surfaces. based on minimum inhibitory concentration (mic) testing with morganella morganii nbrc3848, raoultella planticola nbrc3317 and enterobacter aerogenes nctc10006, we screened nine food additives and four detergents with relatively high inhibitory potency. we prepared food contact material surfaces contaminated with histamine-producing bacteria, and dipp ...201122200801
Inhibitory effect of essential oils, food additives, peracetic Acid and detergents on bacterial of histidine decarboxylase.The aim of this study is to examine whether various essential oils, food additives, peracetic acid and detergents inhibit bacterial histidine decarboxylase. Crude extract of Morganella morganii NBRC3848 was prepared and incubated with various agents. Histidine decarboxylase activity was significantly inhibited (p<0.05) by 26 of 45 compounds tested. Among the 26 agents, sodium hypochlorite completely decomposed both histidine and histamine, while peracetic acid caused slight decomposition. Histid ...201122200744
sitafloxacin: in bacterial infections.sitafloxacin is a fluoroquinolone antibacterial with in vitro activity against a broad range of gram-positive and -negative bacteria, including anaerobic bacteria, as well as against atypical pathogens. it is approved in japan for use in a number of bacterial infections caused by sitafloxacin-susceptible strains of staphylococcus spp., streptococcus pneumoniae, other streptococcus spp., enterococcus spp., moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., enterobacter sp ...201121504249
identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal rna gene sequence analysis.clin microbiol infect 2011; 17: 776-779 abstract: dna sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16s and internal transcribed spacer (its) ribosomal rna gene sequence analysis. pcr was positive for 24 of 99 samples tested. both culture and 16s rdna sequence analysis identified pseudomonas aeruginosa, streptococci and enterobacteriaceae. isolates misidentified as burkholderia cepacia by biochemical tes ...201121521414
[adhesive properties of microorganisms colonizing rheumatoid arthritis and systemic lupus erythematosus patients].evaluation of adhesive properties of microorganisms colonizing rheumatoid arthritis and systemic lupus erythematosus patients.201121598617
[beta-lactamases of clinical isolates of enterobacteriaceae family].among 62 clinical strains of enterobacteria, isolated from intestinal tract, representatives of genus escherichia made--67.4%, klebsiella--4.84%, citrobacter--4.84%, enterobacter--8.06%, proteus--6.45%, morganella of 8.06%. preparations of fluorquinolone group--gatifloxacin and moxifloxacin, the number of steady strains being from 0.0% to 33.3%, were the most effective in respect of enterobacteria the investigated strains of enterobacteria showed considerable resistance to beta-lactam antibiotic ...201121598655
new delhi metallo-ss-lactamase-1: local acquisition in ontario, canada, and challenges in detection.new delhi metallo-ß-lactamase-1 (ndm-1) is a recently identified metallo-ß-lactamase that confers resistance to carbapenems and all other ß-lactam antibiotics, with the exception of aztreonam. ndm-1 is also associated with resistance to many other classes of antibiotics. the enzyme was first identified in organisms isolated from a patient in sweden who had previously received medical treatment in india, but it is now recognized as endemic throughout india and pakistan and has spread worldwide. t ...201121624908
[urinary tract infection in patient after brain injury].intruduction: one of the most important problems in treating patients after head injury is urinary complication. the first place take urinary infections, which disturb rehabilitation process. clean intermittent catherization, bladder training and good care do not preserve infection. the purpose of our study was to present the frequency of urinary infections and main pathogens involved in these infections among patients treated in the department of rehabilitation after head injury.201121812229
new delhi metallo-ss-lactamase-1: detection and prevention. 201121788418
bacterial infection in association with snakebite: a-á10-year experience in a northern taiwan medical center.background: microbiological data of secondary wound infections following snakebites is rarely reported in taiwan. the objective of this study was to assess the secondary wound infection after venomous snakebites. methods: we conducted a 10-year retrospective survey on patients admitted for venomous snakebites and microbiological data of wound cultures at a medical center in northern taiwan. results: between april 2001 and april 2010, 231 patients who experienced snakebites were included. male pr ...201121700517
Genus-wide physicochemical evidence of extracellular crystalline silver nanoparticles biosynthesis by Morganella spp.This study was performed to determine whether extracellular silver nanoparticles (AgNPs) production is a genus-wide phenotype associated with all the members of genus Morganella, or only Morganella morganii RP-42 isolate is able to synthesize extracellular Ag nanoparticles. To undertake this study, all the available Morganella isolates were exposed to Ag+ ions, and the obtained nanoproducts were thoroughly analyzed using physico-chemical characterization tools such as transmission electron micro ...201121713008
quantification of total and specific gram-negative histamine-producing bacteria species in fish using an mpn real-time pcr method.quantification of histamine-producing bacteria (hpb) is necessary in order to elucidate the role that hpb play in scombrotoxin (histamine) fish poisoning. we report here the evaluation of a real-time pcr method for the quantification of total and specific gram-negative hpb species in fish using a most probable number (mpn) format. the species-specific real-time pcr assay was 100% inclusive for independently detecting morganella morganii, enterobacter aerogenes, raoultella planticola/ornithinolyt ...201121839377
identification of bla (kpc-2) on different plasmids of three morganella morganii isolates.three morganella morganii strains resistant to carbapenems were recovered from the surgical intensive care unit (sicu) in our hospital. carbapenemases and extended-spectrum β-lactamases (esbls) were respectively detected by the modified hodge test and the modified clinical and laboratory standards institute (clsi) esbl confirmatory test in all isolates. amplification of whole-cell and plasmid dnas extracted from isolates with primers specific for the bla (kpc) produced an amplicon confirmed to b ...201121874606
first reported fatal morganella morganii infections in chickens.morganella morganii, a gram-negative rod commonly found in the intestines of humans and other animals, is here confirmed to cause a fatal infection in chickens by isolation and identification of the bacteria, 16s rrna gene sequencing, and experimental infection. this is the first case of m. morganii infection in chickens.201122176761
[antimicrobial sensitive of morganella morganii].the aim of this study was the evaluation of the antimicrobial sensitive of morganella morganii rods isolated from clinical samples. this study included 50 of m. morganii strains isolated in the clinical microbiology department of dr. a. jurasz university hospital in 2008-2009. all of strains were sensitive to carbapenems (imipenem, meropenem, ertapenem, doripenem) and piperacillin/tazobactam and most of them to beta-lactam antibiotics, aminoglycosides and fluorochinolons. resistance to tetracycl ...201122184910
antibacterial activity of essential oil vapor for histamine-producing bacteria.in this study, we evaluated the antibacterial activity of essential oil vapors against histamine-producing bacteria morganella morganii nbrc3848 and raultella planticola nbrc3317. we measured the minimum inhibitory dose (mid) of 14 essential oils towards these two strains. allyl isothiocyanate (ait) and salicylaldehyde (sa) vapors showed higher antibacterial activity than the other 12 essential oil vapors. both ait and sa vapors suppressed growth of total aerobic bacteria and histamine-producing ...201122200745
metallo-β-lactamases among enterobacteriaceae from routine samples in an italian tertiary-care hospital and long-term care facilities during 2008.the emergence of metallo-β-lactamase (mbl)-producing enterobacteriaceae is a serious public health concern. producers have been repeatedly isolated from patients and long-term care facility (ltcf) residents around bolzano, and we sought to assess their prevalence and clinical impact. all routine enterobacteriaceae isolates from a bolzano tertiary-care hospital and associated long-term care facilities in 2008 (n = 5500) were screened for mbls, with case details reviewed for the source patients. i ...201120345467
infected abdominal aortic aneurysm due to morganella morganii: ct findings.an infected aortic aneurysm, or mycotic aneurysm, is a rare arterial dilatation due to destruction of the infected vessel wall. common pathogens resulting in an infected aortic aneurysm are salmonella and clostridium species, as well as staphylococcus aureus; morganella morganii, on the other hand, is very rare. an infected abdominal aortic aneurysm has tendencies to grow rapidly and to rupture. the mortality rate is high in patients undergoing emergent surgical intervention. we report the case ...201120352211
Bacterial infections of Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to gamete collecting weirs in Michigan.Herein, we describe the prevalence of bacterial infections in Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to spawn in two tributaries within the Lake Michigan watershed. Ten bacterial genera, including Renibacterium, Aeromonas, Carnobacterium, Serratia, Proteus, Pseudomonas, Hafnia, Salmonella, Shewanella and Morganella, were detected in the kidneys of Chinook salmon (n = 480) using culture, serological and molecular analyses. Among these, Aeromonas salmonicida was detected at ...201222168454
pcr detection and identification of histamine-forming bacteria in filleted tuna fish samples.total of 14 filleted yellowfin tuna fish (thunnus albacares) sold in wholesale fish market and supermarkets in milan, italy, were purchased and tested to determine microbial count, histamine level, histamine-forming bacteria, and their ability to produce histamine in culture broth. although histamine level was less than 10 ppm, many samples showed high total viable bacterial and enterobacterial counts that reached dangerous levels after temperature abuse for short periods of time. a pcr assay ta ...201222251187
species-specific dna probe and development of a quantitative pcr assay for the detection of morganella morganii.aims: the development of a sybr green quantitative pcr assay (qpcr) for the specific detection of morganella morganii, a a fish pathogen responsible of the histamine fish poisoning. methods and results: a new primer set, amplifying a 179 bp fragment of the 16s rrna gene was selected for specificity, and 14 m. morganii strains and 32 non-morganella strains were evaluated. the melting temperature of 84 °c was consistently specific for the amplicon. two standard curves were constructed: the minimum ...201222251367
antimicrobial susceptibility of gram-negative pathogens isolated from patients with complicated intra-abdominal infections in south african hospitals (smart study 2004-2009): impact of the new carbapenem breakpoints.abstract background: the study for monitoring antimicrobial resistance trends (smart) follows trends in resistance among aerobic and facultative anaerobic gram-negative bacilli (gnb) isolated from complicated intra-abdominal infections (ciais) in patients around the world. methods: during 2004-2009, three centralized clinical microbiology laboratories serving 59 private hospitals in three large south african cities collected 1,218 gnb from complicated intra-abdominal infections (ciais) and t ...201222220506
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