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studies on antimicrobial activity of perionyx excavatus.the antimicrobial activity of vermiextract was assessed against four human pathogenic microorganisms. especially strains of proteus species are a prominent cause of urinary tract infection in children. the present investigation deals with particular urinary tract pathogenic organisms like e. coli, proteus, providencia, morganella species. screening was performed by disc diffusion method. the zone of inhibition range from 17 to 19 mm against 1.0x10(8) cells of each culture at 1 mg concentration o ...200719090177
pathogens in early-onset and late-onset intensive care unit-acquired pneumonia.to compare the type of pathogens isolated from patients with early-onset intensive care unit (icu)-acquired pneumonia with those isolated from patients with late-onset icu-acquired pneumonia and to study risk factors for the isolation of pathogens that are potentially resistant to multiple drugs.200717385143
insecticidal bacteria isolated from predatory larvae of the antlion species myrmeleon bore (neuroptera: myrmeleontidae).various bacterial species were isolated from the crop (digestive organ) of the antlion species myrmeleon bore and tested for their insecticidal activity against caterpillars by injection. sixty-eight isolates from the antlion crop were grouped into twenty-four species based on homologies of 16s rrna gene sequences and biochemical properties. isolated bacillus cereus, bacillus sphaericus, morganella morganii, serratia marcescens and a klebsiella species killed 80% or more cutworms when injected a ...200717399737
population-based laboratory surveillance for tribe proteeae isolates in a large canadian health region.the tribe proteeae comprises the genera proteus, morganella and providencia. few studies have specifically investigated the epidemiology of infections caused by the proteeae, and none has been conducted in a large non-selected population. the present study was a population-based laboratory surveillance in the calgary health region (population 1.2 million), canada during 2000-2005 that aimed to define the incidence, demographical risk-factors for acquisition and antimicrobial susceptibilities of ...200717403132
detection of extended-spectrum beta-lactamases among enterobacteriaceae by use of semiautomated microbiology systems and manual detection procedures.three commercially available microbiology identification and susceptibility testing systems were compared with regard to their ability to detect extended-spectrum beta-lactamase (esbl) production in enterobacteriaceae, i.e., the phoenix automated microbiology system (bd diagnostic systems, sparks, md), the vitek 2 system (biomérieux, marcy l'etoile, france), and the microscan walkaway-96 system (dade behring, inc., west sacramento, ca), using routine testing panels. one hundred fifty putative es ...200717287329
cervical abscess with vaginal fistula after extraperitoneal cesarean section.extraperitoneal cesarean section was once used for the prevention of infection and postoperative adhesion. however, we report an unusual complication after this procedure. a 29-year-old woman had pus discharge from the anterior vaginal wall after extraperitoneal cesarean section. broad-spectrum antibiotics failed to relieve her symptoms and vaginal culture yielded morganella morganii . magnetic resonance imaging, sagittal view, showed a cervical abscess measuring 5 x 5 cm with a tract extending ...200718194912
increased serum iga and igm against lps of enterobacteria in chronic fatigue syndrome (cfs): indication for the involvement of gram-negative enterobacteria in the etiology of cfs and for the presence of an increased gut-intestinal permeability.there is now evidence that chronic fatigue syndrome (cfs) is accompanied by immune disorders and by increased oxidative stress. the present study has been designed in order to examine the serum concentrations of iga and igm to lps of gram-negative enterobacteria, i.e. hafnia alvei; pseudomonas aeruginosa, morganella morganii, proteus mirabilis, pseudomonas putida, citrobacter koseri, and klebsiella pneumoniae in cfs patients, patients with partial cfs and normal controls. we found that the preva ...200717007934
albomycin is an effective antibiotic, as exemplified with yersinia enterocolitica and streptococcus pneumoniae.albomycin belongs to the class of sideromycins, compounds composed of iron carriers linked to antibiotic moieties. albomycin was found to be active against bacteria that have a functional ferric hydroxamate transport system meaning that bacteria will actively transport albomycin until they die. we examined the activity spectrum of albomycin for bacterial pathogens and found that enterobacteriaceae except species of proteus and morganella were sensitive. resistance in the two genera was due to th ...200717459767
[purification and characterization of carbonyl enantioselective reductase from morganella morganii j-8].the purification and the characteristics of an enzyme from morganella morganii j-8, which could produce d-pseudoephedrine from 1-phenyl-2-methylamine-acetone, were performed in this study. in this research, first, cells were disrupted by ultrasonic treatment at 4 degrees c. the carbonyl enantioselective reductase was purified with a combination of ammonium precipitation, phenyl superose hydrophobic chromatography, deae anion exchange, and native polyacrylamide gel electrophoresis. the molecular ...200717460900
thirteen-lined ground squirrels (spermophilus tridecemlineatus) harbor multiantibiotic-resistant bacteria.whether wild-caught animals used for biomedical research carry antibiotic-resistant bacteria is not well studied. thirteen- lined ground squirrels (spermophilus tridecemlineatus) are small mammals used to study hibernation. these animals are captured from the wild or are born in laboratory animal facilities to wild-caught mothers. because microorganisms harbored by 13-lined ground squirrels may be pathogenic to their caretakers and other laboratory animals, learning more about antibiotic resista ...200717487947
[genetic markers of pathogenicity of conditionally pathogenic enterobacteria isolated from children with diarrheal diseases].in the process of examination of 89 children from different age groups with diarrheal disease caused by bacteria from enterobacteriaceae family 89 microorganisms were isolated including klebsiella spp. (37 isolates), citrobacter spp. (9 isolates), enterobacter spp. (17 isolates), hafnia alvei (1 isolate), morganella morganii (11 isolates), proteus spp. (14 isolates). presence of genes associated with pathogenicity islands (pais): hlya, hlyb (hemolysin), sfag (fimbria antigen type s), cnf1 (cytot ...200717523471
in vitro activity of ceftaroline (ppi-0903m, t-91825) against bacteria with defined resistance mechanisms and phenotypes.ceftaroline (ppi-0903m, t-91825) is a novel cephalosporin, administered as an n-phosphono prodrug. we investigated its in vitro activity and resistance selection potential.200717548456
gene organization of the ornithine decarboxylase-encoding region in morganella morganii.the production of putrescine is a relevant property related to food quality and safety. morganella morganii is responsible for putrescine production in fresh fish decomposition. the aim of this study was to gain deeper insights into the genetic determinants for putrescine production in m. morganii.200717578420
prevalence, microbiology, and clinical characteristics of extended-spectrum beta-lactamase-producing enterobacter spp., serratia marcescens, citrobacter freundii, and morganella morganii in korea.we examined the prevalence and characteristics of extended-spectrum beta-lactamase (esbl)-producing clinical isolates among enterobacter spp., serratia marcescens, citrobacter freundii, and morganella morganii, and evaluated screening criteria, clinical characteristics and outcomes of infections caused by esbl-producing organisms. between january and june 2005, a total of 493 nonduplicate consecutive isolates were collected at asan medical center, a 2,300-bed tertiary hospital in seoul, republic ...200717587073
changes in histamine and microbiological analyses in fresh and frozen tuna muscle during temperature abuse.temperature abuse of tuna (thunnus alalunga) was carried out in order to assess the histamine buildup in fish-processing facilities where fish can be exposed to high temperatures for short periods of time. histamine production was studied in tuna loins under different storage and abuse conditions. tuna was stored at 0-2 degrees c, 3-4 degrees c, and 6-7 degrees c, and abused for 2 h daily at 20 degrees c and 30 degrees c for 7-12 days. loins abused at 30 degrees c for 2 h daily contained potenti ...200717613069
ctx-m-3-type extended-spectrum beta-lactamase-producing morganella morganii: first description of an isolate from turkey. 200717644345
diversity of microflora in the gut and casts of tropical composting earthworms reared on different substrates.the diversity of fungi, bacteria, yeast, actinomycetes and protozoa were analysed in the gut and casts of eudrilus eugeniae, lampito mauritii, eisenia fetida and perionyx excavatus, both qualitatively and quantitatively as influenced by different feed substrates like clay loam soil, cowdung and pressmud. while actinomycetes (streptomyces albus, s. somaliensis, nocardia asteroides, n. caviae and saccharomonosporia) were not digested by any of these species of worms, protozoa (amoeba proteus, a. t ...200717717992
first report of the emergence of ctx-m-type extended-spectrum beta-lactamases (esbls) as the predominant esbl isolated in a u.s. health care system.ctx-m-type extended-spectrum beta-lactamases (esbls) have become increasingly common worldwide, with the notable exception of the united states, where tem- and shv-type esbls have appeared to predominate. we have noted the emergence of esbls in our health care system (the university health system in san antonio, tx), especially in escherichia coli isolates, that preferentially hydrolyze cefotaxime rather than ceftazidime, suggesting the possibility of ctx-m-type enzymes. microbiology laboratory ...200717724160
[waterhouse-friderichsen syndrome associated to a morganella morganii and enterococcus faecium peritonitis].about fifty to sixty percent of patients with septic shock acquire acute adrenal insufficiency. this insufficiency is most often relative, but can sometimes be absolute. bilateral adrenal haemorrhage is a rare aetiology of absolute acute adrenal insufficiency. it is classically described in patients with severe meningococcemia (purpura fulminans), who commonly present many of the risk factors associated with bilateral adrenal haemorrhage (shock, coagulation disorders, sepsis). we report a case o ...200717766079
[the antibacterial activity of fluoroquinolones against gram-negative bacteria isolated from gifu prefecture (2005)].we investigated the susceptibility to 6 fluoroquinolones against 433 strains of gram-negative bacteria isolated from 6 medical facilities in gifu prefecture between january and september in 2005, determined by the agar dilution methods in according with the japan society of chemotherapy. we also investigated the correlation between the degree of resistance to fluoroquinolones and the amino acid substitutions in the quinolone resistance-determining region (qrdr). the tested clinical isolates were ...200717867488
epidemiology and outcomes of multiple antibiotic-resistant bacterial infection in renal transplantation.mutiresistant bacterial infections are an emerging problem in the nosocomial setting. our objectives were to describe the incidence, outcome, and risk factors for acquisition of multiresistant bacteria among renal transplant recipients.200717889144
bacterial gangs attack implants. 200717918118
isolation of morganella morganii from a domestic rabbit with bronchopneumonia. 200717938414
anti-amebic antibody activity in patients, determined with antigens prepared from virulent parasites (indirect hemagglutination assay and enzyme-linked immunosorbent assay).the serology of amebiasis is affected by low sensitivity and specificity.200717939629
species interactions in mixed-community crystalline biofilms on urinary catheters.previous experimental investigations of the crystalline biofilms that colonize and block urinary catheters have focussed on their formation by pure cultures of proteus mirabilis. in the urine of patients undergoing long-term catheterization, p. mirabilis is commonly found in mixed communities with other urinary tract pathogens. little is known about the effect that the other species have on the rate at which p. mirabilis encrusts catheters. in the present study, a set of data on the nature of th ...200717965358
multiple antimicrobial resistance in enterobacteriaceae isolates from pristine freshwater.a freshwater enterobacterial population (n = 111) was studied for antimicrobial and mercury resistance patterns, and for its possible association with biotic and abiotic factors in that environment. conventional biochemical tests identified klebsiella sp, morganella sp, serratia sp, escherichia sp, enterobacter sp, edwarsiella sp, proteus sp, citrobacter sp, providencia sp, and kluyvera sp. there was no correlation between antimicrobial resistance patterns of isolates and bacterial genera, but r ...200717985304
bacterial translocation in small intestinal ischemia-reperfusion injury and efficacy of anti-cinc antibody treatment.the involvement of bacterial translocation in small intestinal ischemia-reperfusion injuries and the efficacy of using anti-cinc antibodies for treatment were investigated. a model for ischemia-reperfusion injury of the small intestine was constructed by clamping the supramesenteric artery (for 90 min) in rats. anti-cinc antibodies and saline were given just before the induction of ischemia in the treatment group and the control group, respectively. six hours after reperfusion, bacteria were det ...200717337893
characterization of in3mor, a new integron carrying vim-1 metallo-beta-lactamase and sat1 gene, from morganella morganii.a carbapenem-resistant morganella morganii clinical isolate that was phenotypically metallo-beta-lactamase (mbl)-positive was recovered from a greek patient. the aim of the study was to analyse the structure of the integron containing the mbl gene.200717341470
antimicrobial activity and pollen composition of honey samples collected from different provinces in turkey.the antibacterial activity of honey samples from different sources were collected and investigated against bacillus cereus, staphylococcus aureus atcc 25923, pseudomonas aeruginosa atcc 27853, klebsiella pneumoniae atcc 27736, morganella morganii, micrococcus luteus nrrl b-4375, escherichia coli atcc 35218, and candida albicans. pathogens exhibited different sensitivities towards the honey samples. the results showed that majority of the honey samples (75%) generally inhibitied the bacteria test ...200717365707
primary urinary tract infection in infants: prophylaxis for uncomplicated pyelonephritis.urinary tract infection (uti) is one of the most common causes of unexplained fever in infants with a reported prevalence range of 5-11%. the clinical and laboratory findings were reviewed, and diagnosis and treatment for 95 infants with primary uti were evaluated in this study.200717371343
evaluation of a new selective chromogenic agar medium for detection of extended-spectrum beta-lactamase-producing enterobacteriaceae.a novel chromogenic agar medium (esbl-bx; biomérieux, marcy l'etoile, france) was compared to macconkey agar supplemented with 2 mg ceftazidime/liter (mckc) for the selective isolation and presumptive identification of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae directly from clinical samples. of a total of 644 clinical specimens (including 551 fecal samples), 496 yielded no growth and 148 yielded growth on one or both media. overall, 44 esbl-producing enterobacteriaceae ...200717182756
histidine decarboxylases and their role in accumulation of histamine in tuna and dried saury.histamine-producing bacteria (hpb) such as photobacterium phosphoreum and raoultella planticola possess histidine decarboxylase (hdc), which converts histidine into histamine. histamine fish poisoning (hfp) is attributable to the ingestion of fish containing high levels of histamine produced by hpb. because freezing greatly decreases the histamine-producing ability of hpb, especially of p. phosphoreum, it has been speculated that hfp is caused by hdc itself from hpb cells autolyzing during froze ...200717220267
tension pyopneumothorax. 200717254692
high-level vancomycin-resistant staphylococcus aureus isolates associated with a polymicrobial biofilm.glycopeptides such as vancomycin are the treatment of choice for infections due to methicillin-resistant staphylococcus aureus. this study describes the identification of high-level vancomycin-resistant s. aureus (vrsa) isolates in a polymicrobial biofilm within an indwelling nephrostomy tube in a patient in new york. s. aureus, enterococcus faecalis, enterococcus faecium, micrococcus species, morganella morganii, and pseudomonas aeruginosa were isolated from the biofilm. for vrsa isolates, vanc ...200717074796
in vitro activities of tigecycline combined with other antimicrobials against multiresistant gram-positive and gram-negative pathogens.to test the activity of tigecycline combined with 16 antimicrobials in vitro against 22 gram-positive and 55 gram-negative clinical isolates.200818033780
early and late endophthalmitis following trabeculectomy in a chinese population.to investigate the rate of bleb-related endophthalmitis over 5 years in a chinese population. methods.:retrospective chart review.200818203087
phylogenetic relationships among species and genera of lycoperdaceae based on its and lsu sequence data from north european taxa.phylogenetic relationships, limits of species, and genera within lycoperdaceae, were inferred by use of its and lsu nu-rdna sequence data. lycoperdaceae was confirmed as monophyletic, and mycenastrum corium as a sister taxon to the ingroup. four major clades were identified and received weak to moderate support and correspond with the genera lycoperdon, bovista, calvatia, and disciseda. the lycoperdon clade includes species from lycoperdon, vascellum, morganella, handkea, bovistella, and calvati ...200818207380
chemical markers for the evaluation of raw material hygienic quality in egg products.the aim of this research was to study uracil and lactic and acetic acids as chemical markers for hygienic quality evaluation of raw material in liquid pasteurized egg products. uracil, absent in sound whole eggs, was formed in raw and pasteurized egg products as a consequence of high microbial contamination (>10(6) cfu/g) after a sufficient lag time, remaining stable at 4 degrees c but disappearing after 7 days of storage at 25 degrees c. both lactic and acetic acids, starting from initial value ...200818211016
the gut-brain barrier in major depression: intestinal mucosal dysfunction with an increased translocation of lps from gram negative enterobacteria (leaky gut) plays a role in the inflammatory pathophysiology of depression.there is now evidence that major depression (mdd) is accompanied by an activation of the inflammatory response system (irs) and that pro-inflammatory cytokines and lipopolysacharide (lps) may induce depressive symptoms. the aim of the present study was to examine whether an increased gastrointestinal permeability with an increased translocation of lps from gram negative bacteria may play a role in the pathophysiology of mdd. toward this end, the present study examines the serum concentrations of ...200818283240
diverse antimicrobial killing by enterococcus faecium e 50-52 bacteriocin.an effective bacteriocin was identified and characterized. lactic acid bacteria were screened against campylobacter jejuni. one bacteriocin producer, enterococcus faecium (nrrl b-30746), was studied. the isolate was grown, and the bacteriocin was purified to single-band homogeneity. biochemical traits indicated that the peptide was a class iia bacteriocin, and it was named e 50-52. the bacteriocin had a molecular weight of 3339.7 and an isoelectric point of 8.0. the minimal inhibitory concentrat ...200818293921
surveillance for plasmid-mediated quinolone resistance determinants in enterobacteriaceae within the calgary health region, canada: the emergence of aac(6')-ib-cr.a study was designed to determine the prevalence of plasmid-mediated quinolone resistance (pmqr) determinants among clinical isolates of enterobacteriaceae from the calgary health region (chr).200818296438
morganella morganii-associated arthritis in a diabetic patient.this case report involves a 60-year-old diabetic man who developed septic arthritis as a result of the pathogen morganella morganii. the patient had complaints of elevated body temperature, malaise, rigors and pain in the left knee, despite no history of trauma. on examination of the knee, erythema, warmth, tenderness and swelling was observed. arthrocentesis performed on his left knee indicated the presence of straw-coloured, cloudy fluid without crystals. bacterial identification based on bioc ...200818327547
secondary structure of its2 rrna provides taxonomic characters for systematic studies--a case in lycoperdaceae (basidiomycota).the secondary structure of the its2 rdna transcript (pre-rrna) could provide information for identifying homologous nucleotide characters useful for cladistic inference of relationships. such structure data could become taxonomic characters. this work compares the effect of several modern nucleotide alignment strategies, including those making use of structure data, on phylogenetic inference. from both the phylogenetic analyses and comparative secondary structure, implications for taxonomy and e ...200818342242
characterization of a second ornithine decarboxylase isolated from morganella morganii.the genes involved in the putrescine formation by morganella morganii were investigated because putrescine is an indicator of food process deterioration. we report here on the existence of a new gene for ornithine decarboxylase (odc) in m. morganii. the sequenced 5,311-bp dna region showed the presence of four complete and one partial open reading frame. putative functions have been assigned to several gene products by sequence comparison with the proteins included in the databases. the third op ...200818389719
in vitro interaction between mecillinam and piperacillin-tazobactam in the presence of azithromycin against members of the enterobacteriaceae family and pseudomonas aeruginosa.mecillinam was tested in vitro alone or in combination with piperacillin-tazobactam and azithromycin against representative species of the enterobacteriaceae family and pseudomonas aeruginosa to extend its antibacterial spectrum, and to protect mecillinam from inactivating enzymes taking advantage of the presence of tazobactam. drug interactions were studied by microdilution method, by selection of spontaneous resistant mutants on agar plates containing the drugs in combination and by time kill ...200818437840
[case of the month; purple urine bag syndrome (pubs)]. 200818460735
effects of rifaximin on bacterial virulence mechanisms at supra- and sub-inhibitory concentrations.rifaximin, a poorly absorbed rifamycin derivative, exhibited time-dependent bactericidal activity and at concentrations as low as 1/32 of the minimum inhibitory concentration (mic) caused morphological alterations in both susceptible and resistant bacterial strains. spontaneous rifaximin-resistant clones appeared with an incidence of 2.6 x 10(-7). the percentage of escherichia coli cells cured of various plasmids ranged from: 4.5-70% (flac), 0-18% (pbp507), 7.7-43.8% (plasmid carrying esbl genes ...200818467244
non-pseudomonal ecthyma gangrenosum present in diclofenac-induced agranulocytosis. 200818474477
extracellular synthesis of crystalline silver nanoparticles and molecular evidence of silver resistance from morganella sp.: towards understanding biochemical synthesis mechanism.there has been significant progress in the biological synthesis of nanomaterials. however, the molecular mechanism of synthesis of such bio-nanomaterials remains largely unknown. here, we report the extracellular synthesis of crystalline silver nanoparticles (agnps) by using morganella sp., and show molecular evidence of silver resistance by elucidating the synthesis mechanism. the agnps were 20+/-5 nm in diameter and were highly stable at room temperature. the kinetics of agnps formation was in ...200818491326
enterobacteriaceae and related organisms isolated from shell eggs collected during commercial processing.in the united states, commercial shell eggs are washed and graded before retail. since passage of the egg products inspection act in 1971, processing guidelines have been set to ensure that external and internal characteristics are maintained. however, less is known about how commercial processing affects the safety of shell eggs. to identify enteric bacteria entering plants and persisting throughout processing, eggs were collected from 3 us commercial shell egg-processing plants on 3 separate v ...200818493013
emergence of antibiotic resistance during therapy for infections caused by enterobacteriaceae producing ampc beta-lactamase: implications for antibiotic use.enterobacter spp., serratia marcescens, citrobacter freundii, and morganella morganii are characterized by chromosomally encoded ampc beta-lactamases and possess the ability to develop resistance upon exposure to broad-spectrum cephalosporins. to determine the incidences of the emergence of resistance during antimicrobial therapy for infections caused by these organisms and the effect of the emergence of resistance on patient outcomes, all patients who were admitted to the asan medical center (s ...200818086837
isolation and purification of enterocin e-760 with broad antimicrobial activity against gram-positive and gram-negative bacteria.strain nrrl b-30745, isolated from chicken ceca and identified as enterococcus durans, enterococcus faecium, or enterococcus hirae, was initially identified as antagonistic to campylobacter jejuni. the isolate produced a 5,362-da bacteriocin (enterocin) that inhibits the growth of salmonella enterica serovar enteritidis, s. enterica serovar choleraesuis, s. enterica serovar typhimurium, s. enterica serovar gallinarum, escherichia coli o157:h7, yersinia enterocolitica, citrobacter freundii, klebs ...200818086839
biodegradation of reactive blue 59 by isolated bacterial consortium pmb11.morphologically different, three bacterial strains, capable of decolorizing reactive blue 59 were isolated from dye effluent contaminated soil sample, collected from ichalkaranji, india. the individual bacterial strains viz. bacillus odysseyi suk3, morganella morganii suk5 and proteus sp. suk7 decolorized reactive blue 59 (50 mg l(-1)) completely within 60, 30, 24 h, respectively, while the bacterial consortium pmb11 of these strains required 3 h for the complete decolorization. the decolorizati ...200818661161
[intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees].dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ...200818683661
growth, inactivation and histamine formation of morganella psychrotolerans and morganella morganii - development and evaluation of predictive models.mathematical models for growth, heat inactivation and histamine formation by morganella psychrotolerans and morganella morganii were studied to evaluate the importance of these bacteria in seafood. curves for growth and histamine formation by m. psychrotolerans in broth and seafood were generated at constant and changing storage temperatures (n=12). observed and predicted times to formation of 100, 500 and 2000 ppm histamine were used for evaluation of an existing m. psychrotolerans histamine fo ...200818845350
modelling the effect of temperature, carbon dioxide, water activity and ph on growth and histamine formation by morganella psychrotolerans.a mathematical model was developed to predict growth and histamine formation by morganella psychrotolerans depending on temperature (0-20 degrees c), atmosphere (0-100% co2), nacl (0.0-6.0%) and ph (5.4-6.5). data from experiments with both sterile tuna meat and luria bertani broth was used to develop the mathematical growth and histamine formation model. the expanded logistic model with a growth dampening parameter (m) of 0.7 was used as primary growth model. a primary model for histamine forma ...200818845351
highly resistant microorganisms in a teaching hospital: the role of horizontal spread in a setting of endemicity.to determine the incidence density of highly resistant organisms (hros) and the relative contribution of horizontal spread in a setting of endemicity.200818973456
[nationwide susceptibility surveillance of ciprofloxacin and various parenteral antimicrobials against bacteria isolated from patients with severe infections--third ciproxan injection special survey (2005)].we conducted 3 nationwide surveillance studies between 2001 and 2005 at 39 participating institutions throughout japan according to the special survey plan to investigate susceptibility to ciprofloxacin (cpfx) and various parenteral antimicrobials using clinical isolates from patients with severe infection during the reexamination period of parenteral cpfx. results of the first special survey (2001) were already reported in this journal. the current third special survey (2005) was conducted at 3 ...200819024645
[biochemical and bacteriological investigation of six cases of purple urine bag syndrome (pubs) in a geriatric ward for dementia].purple urine bag syndrome is a condition in which the urinary catheter bag turns purple. a tryptophan-indigo hypothesis has been proposed as the mechanism of pubs, in which bacterial decomposition of tryptophan in gut associated with chronic constipation, bacterial overgrowth in the urinary tract and alkaline urine causes production of indigo and discoloration. we considered that further investigation of cases was needed.200819057104
study on the bacterial midgut microbiota associated to different brazilian populations of lutzomyia longipalpis (lutz & neiva) (diptera: psychodidae).the bacterial community associated with the midgut of three brazilian lutzomyia longipalpis (lutz & neiva) populations, two from endemic areas for visceral leishmaniasis (jacobina, bahia state and são luís, maranhão state) and one from a non-endemic area (lapinha cave, minas gerais state), was identified. five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. the species identification was based on molecular and traditional ba ...200819061048
[detection of ampc beta-lactamases in gram-negative bacteria isolated from urine].ampc beta-lactamases, enzymes produced by a range of gram-negative bacteria and able to hydrolyse penicillins, monobactams, cephalosporins and cephamycins, are not inhibited by beta-lactamase inhibitors. they are spread via plasmids and pose a huge epidemiological and diagnostic problem. the production of ampc b-lactamases was tested in various gram-negative bacteria. of strains isolated from urine of patients diagnosed with urinary tract infection, we selected those that were resistant to third ...200819069027
normalization of leaky gut in chronic fatigue syndrome (cfs) is accompanied by a clinical improvement: effects of age, duration of illness and the translocation of lps from gram-negative bacteria.there is now evidence that an increased translocation of lps from gram negative bacteria with subsequent gut-derived inflammation, i.e. induction of systemic inflammation and oxidative & nitrosative stress (io&ns), is a new pathway in chronic fatigue syndrome (cfs).200819112401
tetracycline-resistance genes in gram-negative isolates from estuarine waters.to investigate the diversity and dissemination of tetracycline resistance genes in isolates from estuarine waters.200819120920
comparison of pcr-based molecular markers for the characterization of proteus mirabilis clinical isolates.proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. for epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (pfge) or ribotyping, have been developed for this pathogen. however, these methods are labor intensive a ...200819219283
renal abscess with morganella morganii complicating leukemoid reaction.we report a case of leukemoid reaction (lr) complicating renal abscess caused by morganella morganii infection in an 80-year-old man. on administration, laboratory tests revealed white blood cell count of 76160 /microl and c reactive protein 3.09 mg/dl. although chronic myeloid leukemia was suspected, bcr/abl fusion transcript was not observed. contrast enhanced computer tomography imaging of the abdomen showed abscess in the right kidney. m. morganii was detected repeatedly in material of liqui ...200818176006
[epidemiology of bacterial intrahospital infections in newborns].nosocomial infections are important problem for the modern medicine. especially sensitive category, susceptible to nosocomial infections, are the infants. the aim of the research was to establish an active monitoring over the breaking out of the nosocomial infections in the neonatology department of the gynecology and obstetrics clinic of the university clinical center tuzla (ukc) and to determine the kind of bacteria that causes the nosocomial infections as well as the type of infection accordi ...200819469274
antibiotic resistance of gram negatives isolates from loggerhead sea turtles (caretta caretta) in the central mediterranean sea.previous studies on fish and marine mammals support the hypothesis that marine species harbor antibiotic resistance and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to assess the resistance to antimicrobial agents of gram negative strains isolated from loggerhead sea turtles (carettacaretta). oral and cloacal swabs from 19 live-stranded loggerhead sea turtles, with hooks fixed into the gut, were analyzed. the antimicrobial resistance ...200919473669
design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties.the aims of the present study were to determine the antibacterial and antifungal activity as well as mutagenicity of sechium edule fluid extract and to obtain a pharmaceutical formulation with them. the extract exhibited antimicrobial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, serratia marcescens, morganella morganii, acinetobacter baumannii, pseudomonas aeruginosa, stenotrophomonas maltophilia, candida spp. and aspergillus spp. isolated fr ...200919477252
oral bacterial flora of the chinese cobra (naja atra) and bamboo pit viper (trimeresurus albolabris) in hong kong sar, china.to determine the oral bacterial flora associated with two common local venomous snakes in hong kong, namely the chinese cobra (naja atra) and the bamboo pit viper (trimeresurus albolabris).200919494373
fatal morganella morganii bacteraemia in a diabetic patient with gas gangrene.we report a case of a 60-year-old lady with a history of a heel ulcer that had not responded to antibiotic therapy. this progressed to involve the right leg, which was swollen and erythematous. radiological imaging revealed the presence of gas within the fascial planes. blood cultures on admission yielded morganella morganii. due to the extent of the gas gangrene and her co-morbidities the patient was not suitable for surgical intervention and was treated conservatively with antibiotics. she det ...200919502368
[evaluation of effectiveness and tolerance of treatment with azithromycin 1.5% eye drops in bacterial conjunctivitis].azithromycin is a macrolide class antibiotic, recently adapted for topical use in ophthalmology. it is effective against the most frequent pathogens found in bacterial conjunctivitis, gram positive and gram negative bacteria. azithromycin has the specificity to have sustained high tissue levels: after repeated instillation, it has been shown to reach sustained concentrations above the mics of susceptible bacteria for 4 days in tears and for 7 days in conjunctiva.200919517846
crystalline bacterial biofilm formation on urinary catheters by urease-producing urinary tract pathogens: a simple method of control.the problem of catheter encrustation stems from infection by urease-producing bacteria. these organisms generate ammonia from urea, elevate the ph of urine and cause crystals of calcium and magnesium phosphates to form in the urine and the biofilm that develops on the catheter. in this study, a laboratory model was used to compare the ability of 12 urease-positive species of urinary tract pathogens to encrust and block catheters. proteus mirabilis, proteus vulgaris and providencia rettgeri were ...200919556373
investigation of shell disease in map turtles (graptemys spp.).nineteen map turtles (graptemys spp.) maintained under natural conditions were investigated because of chronic shell abnormalities. animals were evaluated using a novel shell scoring system that divided the 54 scutes into six regions, with each region scored for lesion extent and severity, and summated to produce a total shell disease score (tsds). complete blood counts and various biochemistry analytes (total protein, albumin, globulin, urea, uric acid, 25-hydroxycholecalciferol, phosphorus, an ...200919617474
[multicenter study on the monitoring of in vitro susceptibility to tigeeyeline in santiago, chile].the objective of this multicenter study was to determine tigecycline susceptibility rates, measured by agar diffusion, in nine hospitals in santiago and to compare these rates with other antimicrobials. each center studied 20 strains per month. all intermediate and fully resistant strains as well as 10% of susceptibile strains were also studied by the broth microdilution method. overall, 2301 strains were studied displaying the following susceptibility rates for tigecycline: 100% for streptococc ...200919621154
identification of a modular pathogenicity island that is widespread among urease-producing uropathogens and shares features with a diverse group of mobile elements.pathogenicity islands (pais) are a specific group of genomic islands that contribute to genomic variability and virulence of bacterial pathogens. using a strain-specific comparative genomic hybridization array, we report the identification of a 94-kb pai, designated icepm1, that is common to proteus mirabilis, providencia stuartii, and morganella morganii. these organisms are highly prevalent etiologic agents of catheter-associated urinary tract infections (cauti), the most common hospital acqui ...200919687197
[formation of biofilms by fecal strains of enterobacteria and yeast fungi from candida genus].to assess the ability of bacteria and candida genus' yeast fungi from human fecal microflora to form biofilms.200919715202
extended-spectrum beta-lactamases among enterobacteriaceae isolated in a public hospital in brazil.extended-spectrum beta-lactamases (esbl) in enterobacteria are recognized worldwide as a great hospital problem. in this study, 127 esbl-producing enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at são paulo, brazil, were submitted to analysis by pcr with specific primers for bla shv, bla tem and bla ctx-m genes. from the 127 isolates, 96 (75.6%) klebsiella pneumoniae, 12 (9.3%) escherichia coli, 8 (6.2%) morganella morganii, 3 (2.3%) proteus ...200919739000
needle puncture and transcutaneous bone biopsy cultures are inconsistent in patients with diabetes and suspected osteomyelitis of the foot.needle puncture has been suggested as a method for identifying bacteria in the bones in patients with diabetes with osteomyelitis of the foot. however, no studies have compared needle puncture with concomitant transcutaneous bone biopsy, which is the current standard recommended in international guidelines.200919228109
[susceptibility of enterobacteriaceae rods to selected tetracyclines].the aim of this study was to evaluated the susceptibility of enterobacteriaceae rods to tigecycline, tetracycline and doxycycline. we analysed 121 strains belonged to species as follows: escherichia coli (40 strains), proteus mirabilis (22), enterobacter cloacae (19), klebsiellapneumoniae (18), morganella morgannii (13), serratia marcescens (4), klebsiella oxytoca (3) i proteus vulgaris (2). all strains of e. coli, k. oxytoca, s. marcescens and p. vulgaris were susceptible to tigecycline. twenty ...200920201321
extended spectrum beta-lactamase detection in gram-negative bacilli of nosocomial origin.resistance to third generation cephalosporins by acquisition and expression of extended spectrum beta lactamase (esbl) enzymes among gram-negative bacilli is on a rise. the presence of esbl producing organisms significantly affects the course and outcome of an infection and poses a challenge to infection management worldwide.200920300397
catheter-related urinary tract infection in patients suffering from spinal cord injuries.urinary tract infection is commoner in patients with spinal cord injuries because of incomplete bladder emptying and the use of catheters that can result in the introduction of bacteria into the bladder. 145 patients suffering from spinal cord injuries, admitted to the institute for physical medicine and rehabilitation, centre for paraplegia of the clinical centre of the university of sarajevo, were included. the patients were divided in three groups according to the method of bladder drainage: ...200919284388
separation of viable histamine-producing bacteria from yellowtail meat components by density gradient centrifugation.quantitative separation of live cells from food samples is essential for non-culture methods to be validated. in this viewpoint, the feasibility of density gradient centrifugation (dgc) was demonstrated for the first time using samples of yellowtail meat to which morganella morganii, a histamine producing bacterium had been added. using a ficoll density gradient from 50 to 10 w/v % with 10 w/v % steps, meat-free fractions of m. morganii cells were collected in 20-50 w/v % layers. the total cell ...200919344096
[ability of opportunistic enterobacteria to adapt to different temperatures].to study variability of enzymatic apparatus of opportunistic enterobacteria.200919459471
antimicrobial activity of selected plant species from "the argentine puna" against sensitive and multi-resistant bacteria.the plant species reported here are traditionally used in the "puna" or "altiplano" of argentina for ailments related to bacterial infections. the aim of this study was to evaluate their antimicrobial properties against a panel of sensitive and multi-resistant gram-positive and gram-negative bacteria.200919467313
haemorrhagic bullae associated with a chicken scratch.complicated skin and soft tissue infection caused by a chicken scratch has rarely been reported. a 3-year-old boy who developed haemorrhagic bullae and necrotising fasciitis following a chicken scratch is reported. morganella morganii and enterococci were cultured from the aspirate. he received antimicrobial treatment, surgical debridement and a skin graft, and recovered uneventfully.200919941755
tubo-ovarian abscess with morganella morganii bacteremia.tubo-ovarian abscess caused by morganella morganii is unusual. a 54-year-old menopausal woman visited the emergency room with lower abdominal pain, vomiting, and fever for 4 days. pelvic examination revealed lifting tenderness over the right adnexum without motion tenderness of the uterus. pelvic sonogram revealed a cystic lesion with heterogeneous content in the right ovary. salpingo-oophoritis was suspected. clindamycin and gentamicin were administered intravenously after obtaining blood cultu ...200919949761
[fatal neonatal sepsis caused by vertical transmission of morganella morganii. report of one case].we report a term neonate who developed early-onset sepsis due to morganella morganii. the child was vaginally delivered after a short labor, and presented signs of perinatal asphyxia. blood cultures taken soon after birth and from mother's lochia were positive for this microorganism. the infection was unresponsive to treatment with cefotaxime, to which the microorganism was susceptible, and the infant died at 17 days of age. m morganii is an opportunistic and uncommon pathogen, causing disease m ...200920011962
18-f fluorodeoxyglucose positron emission tomography indicating unsuspected infections in two patients with dermatomyositis.dermatomyositis is an idiopathic inflammatory myopathy that may be associated with malignancies. the technique of 18-f fluorodeoxyglucose (fdg)-positron emission tomography (pet) is an important tool to investigate underlying malignancy in patients with a possible paraneoplastic syndrome. we report two consecutive patients with dermatomyositis in whom 18-f fdg-pet revealed unsuspected infections. physicians should be aware that a positive 18-f fdg-pet is not specific for malignancy and may revea ...200920055842
first case of capd-related peritonitis caused by morganella morganii.morganella morganii, a gram-negative bacillus, is a rare cause of peritonitis. in this article we report a 55-year-old female patient with peritonitis due to morganella morganii who was receiving continuous ambulatory peritoneal dialysis (capd).201020056997
histamine, cadaverine, and putrescine produced in vitro by enterobacteriaceae and pseudomonadaceae isolated from spinach.a total of 364 bacterial isolates, obtained from spinach leaves, were assayed in a decarboxylase broth containing histidine, lysine, and ornithine to check their ability to produce biogenic amines, and then quantified by high-performance liquid chromatography. among these isolates, 240 formed cadaverine, 208 formed putrescine, and 196 formed histamine, in widely varying amounts. they frequently produced more than one biogenic amine. klebsiella pneumoniae subsp. pneumoniae and morganella morganii ...201020132689
differential characterization of biogenic amine-producing bacteria involved in food poisoning using maldi-tof mass fingerprinting.histamine poisoning is caused by the consumption of fish and other foods that harbor bacteria possessing histidine decarboxylase activity. with the aim of preventing histamine formation, highly specific mass spectral fingerprints were obtained from the 16 major biogenic amine-producing enteric and marine bacteria by means of maldi-tof ms analysis. all bacterial strains analyzed exhibited specific spectral fingerprints that enabled its unambiguous differentiation. this technique also identified p ...201020151397
biodegradation studies and sequencing of microcystin-lr degrading bacteria isolated from a drinking water biofilter and a fresh water lake.the presence of microcystin-lr -degrading bacteria in an active anthracite biofilter and in lake mead, nevada was investigated. four bacterial isolates from enrichment culture were identified using 16s rrna analysis. microcystin biodegradation tests were performed with both, the enrichment cultures and the respective isolates, using microcystin alone and acetate as carbon sources. a newly recognized microcystin-degrading bacterium, morganella morganii, was isolated from the biofilter and from la ...201020219514
antibacterial activities of crude extract of aloe barbadensis to clinically isolated bacterial pathogens.the antibacterial activity of aloe barbadensis was tested on clinically isolated bacterial pathogens i.e. enterococcus bovis, staphylococcus aureus, escherichia coli, proteus vulgaris, proteus mirabilis, pseudomonas aeruginosa, morganella morganii, and klebsiella pneumoniae causing infection in human being. ethanolic and aqueous extracts were used for the antibacterial effect, which was measured by the appearance of zone of inhibition. relatively higher mic concentrations were obtained for gram ...201019263248
the conundrum of the gram-positive rod: are we missing important pathogens in complicated skin and soft-tissue infections? a case report and review of the literature.the designation "gram-positive bacillus" includes a variety of pleomorphic microorganisms, including diphtheroids, coryneform species, coccobacilli, and other small rods. despite differing greatly in their virulence, sources, and even genus, these microscopically similar organisms are often difficult to differentiate without genetic testing.201019803730
development of a real-time pcr assay with an internal amplification control for detection of gram-negative histamine-producing bacteria in fish.prompt detection of bacteria that contribute to scombrotoxin (histamine) fish poisoning can aid in the detection of potentially toxic fish products and prevent the occurrence of illness. we report development of the first real-time pcr method for rapid detection of gram-negative histamine-producing bacteria (hpb) in fish. the real-time pcr assay was 100% inclusive for detecting high-histamine producing isolates and did not detect any of the low- or non-histamine producing isolates. the efficienc ...201021356438
development of molecular-based methods for determination of high histamine producing bacteria in fish.histamine (or scombroid) fish poisoning is a significant cause of food borne disease in the united states. in this study, we describe the development of a molecular-based technique which uses digoxigenin (dig) labeled dna probes for the detection of gram negative bacteria producing high amounts of histamine (>1000 ppm). a cocktail of pcr amplification fragments corresponding to a 709 bp fragment of the histidine decarboxylase (hdc) gene of four high producing bacteria (morganella morganii, enter ...201020392504
[bacterial meningitis due to gram-negative bacilli in adults].gram-negative bacilli are causative agents of bacterial meningitis in the neonatal period but rarely cause meningitis in adults without risk factors.201020414871
[meningoencephalitis due to morganella morganii: a case report].a central nervous system infection due to morganella morganii is uncommon. we report a case diagnosed at the neurological department of fann teaching hospital in dakar, senegal. a 12-year-old boy was hospitalized for acute meningoencephalitis. the ct scan was normal and the study of cerebrospinal fluid (csf) revealed cytological and biochemical abnormalities and m. morganii. hiv and syphilitic serologies were negative and blood cd4 lymphocyte count showed 354 per mm(3). the treatment with cefota ...201020431984
communal action of microbial cultures for red he3b degradation.the consortium pmb11 consisting of three bacterial species, originally isolated from dye contaminated soil was identified as bacillus odysseyi suk3, morganella morganii suk5 and proteus sp. suk7. the consortium possessed the ability to decolorize various textile dyes as well as mixtures of dyes. pmb11 could decolorize red he3b (50 mg l(-1)) with 99% of decolorization within 12 h in nutrient broth, while in mineral medium it could decolorize up to 97% within 24h. induction in the activities of va ...201020510505
defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions.a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ...201020537393
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