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antimicrobial activity of dactimicin in vitro compared with that of dibekacin, netilmicin, sisomicin and micronomicin.antimicrobial activity of dactimicin, a pseudo-disaccharide aminoglycoside antibiotic, was compared with those of dibekacin, netilmicin, sisomicin and micronomicin using clinical isolates of four gram-positive and sixteen gram-negative bacteria. dactimicin was more active than the reference amino-glycosides against serratia marcescens, especially gentamicin-resistant serratia sp., proteus vulgaris, p. rettgeri and klebsiella oxytoca, but less active against pseudomonas aeruginosa and p. mirabili ...19892632214
urinary tract infections in children.from january 1981 to december 1987, 346 children with urinary tract infections, proved by urine culture, were admitted to the department of pediatrics, mackay memorial hospital. the ratio of male to female was 3.0 in children below 2 years, and 0.8 in children above 2 years, of age. the urine specimens were collected from suprapubic punctures in 281 cases (81.2%). fever was the most common clinical manifestation. in children below two years old, other common symptoms and signs were body weight l ...19892637602
[bacteriologic study of abscesses caused by bites of snakes of the genus bothrops].the bacterial flora of 99 cases of abscesses following bothrops snakebite were analysed. they corresponded to 61.1% of all snakebite abscesses observed in 1030 patients attending the hospital de doenças tropicais de goiánia in goiás, brazil, from january 1984 to april 1988. an exsudate sample of each abscess was examined by gram stain, culture and susceptibility tests. the gram negative bacillis, morganella morganii, escherichia coli and providencia sp were the most frequent bacterias isolated. ...19892640511
isolation and analysis of the c-terminal signal directing export of escherichia coli hemolysin protein across both bacterial membranes.we have studied the c-terminal signal which directs the complete export of the 1024-amino-acid hemolysin protein (hlya) of escherichia coli across both bacterial membranes into the surrounding medium. isolation and sequencing of homologous hlya genes from the related bacteria proteus vulgaris and morganella morganii revealed high primary sequence divergence in the three hlya c-termini and highlighted within the extreme terminal 53 amino acids the conservation of three contiguous sequences, a pot ...19892656259
discovery of new morganocin types of morganella morganii in strains of diverse serotype and the apparent independence of bacteriocin type from serotype of strains.a collection of 45 serologically distinct strains of morganella morganii from several european countries was typed by morganocin production (p) and sensitivity (s). this permitted their differentiation into 33 morganocin p/s types; 15 new types of morganocin and one new morganocin sensitivity type were found and are defined. morganocin production and sensitivity characteristics appeared to be unrelated to the o and h antigens of the strain. the finest strain recognition in m. morganii might be a ...19892659799
comparison of the in vitro and in vivo antibacterial activities of cefepime (bmy-28142) with ceftazidime, cefuzonam, cefotaxime and cefmenoxime.cefepime (bmy-28142), a new semisynthetic cephalosporin, was evaluated for in vitro and in vivo antibacterial activities in comparison with ceftazidime, cefuzonam, cefotaxime and cefmenoxime. cefepime showed a well-balanced, broad spectrum of activity against a number of clinical isolates collected in japan. the activity of cefepime against gram-positive bacteria was several times greater than that of ceftazidime, nearly comparable to cefotaxime and cefmenoxime, and slightly weaker than cefuzona ...19892663406
[susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates].susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ...19892664255
[evaluation of a new semi-automatic method of identifying enterobacteriaceae, m.i.s.-enterobacteriaceae].m.i.s.-enterobacteriaceae is a new kit for identifying enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. the validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the classical method of identification in test-tubes. results showed a diagnosis accuracy of 96 p. cent at the species level and 97.7 p. cent at the genus level. diagnosis accuracy reac ...19892665580
[properties of bacteriocins of morganella morganii].some properties of morganella morganii bacteriocins were determined. for this purpose two strains (115 and 137) which after mitomycin c induction produced bacteriocins in high titer were chosen. the influence of several physical and chemical factors such as: heating and storage at various temperatures, a freezing and thawing, an influence of buffered fluid of different ph, and digestion by trypsin, papain and lysozyme were investigated. range of bacteriocin activity against various microorganism ...19892668659
proteeae species bacteriuria accompanying proteeae species groin skin carriage in geriatric outpatients.there have been numerous reports of proteeae species urinary tract infections among elderly individuals. to explore a possible urinary carrier source of proteeae species in this population, the frequency of aerobic gram-negative bacillus (agnb) bacteriuria at the greater than or equal to 10(2)/ml level was determined in 67 ambulatory elderly outpatients classified as to proteeae group (morganella, proteus, providencia) groin carriage by a set of two skin cultures obtained at least 1 week apart. ...19892674195
in-vitro activity of fce 22101 and other beta-lactam antibiotics against enterobacteriaceae resistant to third generation cephalosporins.the in-vitro activity of fce 22101 was compared with those of imipenem, aztreonam, cefotaxime, ceftriaxone and latamoxef against 225 enterobacteriaceae (209 clinical isolates and 16 derivatives of escherichia coli k12) chosen for their resistance (mic greater than or equal to 8 mg/l) to third-generation cephalosporins. the beta-lactamases produced by the strains of enterobacter cloacae, klebsiella pneumoniae and esch. coli were identified. the mean mics of fce 22101 were 2-8 mg/l for ent. cloaca ...19892676939
in-vitro activity of meropenem against clinical isolates in a multicentre study in italy.a multicentre in-vitro study was undertaken to evaluate the susceptibility of bacterial pathogens isolated in different italian hospitals to meropenem. a total of 1399 aerobic and 452 anaerobic strains was analysed. comparative agents were imipenem, cefotaxime, ceftazidime, ceftriaxone, piperacillin, ciprofloxacin, gentamicin, amikacin, plus vancomycin when appropriate. the mic ranges (mg/l) of meropenem were: 0.015-2 for klebsiella spp., proteus spp., morganella morganii and providencia spp.; l ...19892681128
pyridoxal 5'-phosphate dependent histidine decarboxylase: overproduction, purification, biosynthesis of soluble site-directed mutant proteins, and replacement of conserved residues.the hdc gene coding for the pyridoxal 5'-phosphate dependent histidine decarboxylase from morganella morganii has been expressed in escherichia coli under control of the lac promoter. the enzyme accumulates to 7-8% of total cell protein and is purified to homogeneity by passage through three columns. fourteen site-directed mutant enzymes were constructed to explore the roles of residues of interest, especially those in the sequence ser229-x230-his231-n epsilon-(phosphopyridoxylidene)lys232, sinc ...19892684275
anti-k1 of the iga class associated with morganella morganii infection.an anti-k1 alloantibody developed in a patient infected with morganella morganii. the serologic behavior and response to dithiothreitol initially suggested that the alloantibody was an igm. however, flow cytometry and the separation of immunoglobulin classes by serum chromatography revealed that the anti-k1 was constituted solely of iga. these data suggest that bacteria-induced red cell antibodies might be of the iga class.19892749877
in vitro activity and beta-lactamase stability of a new carbapenem, sm-7338.sm-7338, a new carbapenem, inhibited most members of the family enterobacteriaceae at mics of 0.015 to 0.25 microgram/ml, including klebsiella oxytoca, citrobacter freundii, enterobacter cloacae, and proteus vulgaris isolates resistant to cefotaxime, ceftazidime, piperacillin, and gentamicin. it was two- to eightfold more active than imipenem, but it inhibited pseudomonas aeruginosa at 1 to 8 micrograms/ml, which was comparable to the activity of imipenem. haemophilus, neisseria, and branhamella ...19892789493
[the ratios and kinds of clinical bacteria isolated in taiwan's large-size hospitals].the prevalence of clinical bacteria, as isolated from linko chang-gung memorial hospital (2,300 beds) in the period january 1985 to december 1986 and from taipei veterans general hospital (2,300 beds) during the period january 1986 to december 1986, was analyzed with the following findings: (i) the isolation ratio of anaerobic and aerobic or facultative bactria during the period of investigation were 7.8% (5,513/70,799) and 92.2% (65,286/70,799), respectively. (ii) of the total aerobic or facult ...19892791722
interactions of meropenem with class i chromosomal beta-lactamases.most newer penicillins and cephalosporins are labile to the class i beta-lactamases that are inducible in pseudomonas aeruginosa. enterobacter spp., citrobacter spp., serratia spp. and indole-positive proteeae, but fail to induce enzyme synthesis below the mic. they remain active against the inducible strains but not against mutants (termed 'stably depressed') that manufacture beta-lactamase copiously without induction. such mutants are segregated at high frequency and may be selected during the ...19892808208
antibacterial activity of tigemonam dicholate (sq 30836) and interaction with beta-lactamases of gram-negative bacteria.sq 30836 is an orally absorbed salt of tigemonam, a new monobactam similar to aztreonam in structure and microbiologic properties. when assayed against 400 clinical isolates, tigemonam's activity was similar to that of aztreonam and carumonam. it was highly effective against enterobacteriaceae but showed poor activity against gram-positive organisms. it inhibited 90% of escherichia coli, klebsiella, shigella, yersinia, proteus, providencia, and morganella strains at 0.5 micrograms/ml or less, an ...19892809698
in vitro activity of tigemonam against urinary tract pathogens.tigemonam's in vitro activity was compared with that of aztreonam, cefotaxime, gentamicin, and norfloxacin. thirty-two strains of pseudomonas and 960 strains of enterobacteriaceae were studied using the agar dilution method (including escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter sp., serratia marcescens, proteus mirabilis, proteus vulgaris, morganella morganii, and salmonella enterica). tigemonam displayed good activity against enterobacteriaceae. minimum inhibitory ...19892809699
in vitro studies of tigemonam: a comparison of the minimum inhibitory and minimum bactericidal concentrations (mic vs mbc).the in vitro activity of tigemonam, a new oral monobactam, was studied with special attention to minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs). against 250 clinical isolates, it inhibited 100% of escherichia sp., klebsiella sp., serratia sp., citrobacter sp., providencia sp., proteus sp., morganella sp., aeromonas sp., yersinia sp., shigella sp., and haemophilus sp. at 0.5 mg/l or less. with 1 mg/l, 75% of enterobacter sp. were inhibited; however, three ...19892809701
[pharmacokinetic and clinical studies of cefteram pivoxil granule in the pediatric field].cefteram pivoxil (cftm-pi), the pivaloyloxymethyl ester of cefteram (cftm) in which aminothiazol was also introduced into the 7 position of cephem nucleus, is a new oral cephem antibiotic. cftm-pi was absorbed through the intestines and hydrolyzed to cftm by esterases in the intestinal wall and existed in the body fluids as cftm. a tablet form of this drug has been released in japan and now a granular form for pediatric patients has been developed. we have determined mics of 5 drugs (cftm, cepha ...19892810762
in vitro evaluation of brl 42715, a novel beta-lactamase inhibitor.the penem brl 42715, c6-(n1-methyl-1,2,3-triazolylmethylene)penem, is a potent inhibitor of a broad range of bacterial beta-lactamases, including the plasmid-mediated tem, shv, oxa, and staphylococcal enzymes, as well as the chromosomally mediated enzymes of bacteroides, enterobacter, citrobacter, serratia, morganella, escherichia, klebsiella, and proteus species. the concentration of brl 42715 needed to reduce the initial rate of hydrolysis of most beta-lactamase enzymes by 50% was less than 0. ...19892817854
ytr-830h: its origin, the present and the future. 198916312317
in vitro evaluation of new beta-lactamase inhibitor: ytr830h and derivatives. 198916312318
[antibacterial activity and beta-lactamase stability of eleven oral cephalosporins].oral cephalosporins (cefixime, cefdinir, cefetamet, ceftibuten, cefpodoxime, loracarbef, cefprozil, cefuroxime, cefaclor, cefadroxil and bay 3522) were compared by their antibacterial profile including stability against new beta-lactamases. both activity and antibacterial spectrum of compounds structurally related to third generation parenteral cephalosporins (of the oximino class) were superior to established compounds. activity against staphylococci was found to be highest for cefdinir, cefpro ...19902079378
a comparison of the mechanisms of decreased susceptibility of aztreonam-resistant and ceftazidime-resistant enterobacteriaceae.twenty five strains of enterobacteriaceae (five each of enterobacter cloacae, citrobacter freundii, serratia marcescens, morganella morganii and providencia stuartii) were exposed to aztreonam and ceftazidime at 1/2 and 1 x mic in liquid medium for 22 h, and also to 3, 5 and 10 x mic and 16 mg/l of each agent in agar. any putative mutant with an increase in the mic of greater than or equal to 4 fold was examined for beta-lactamase expression and outer membrane protein profile. mutants were selec ...19902081717
in vitro antimicrobial activity of defensins against ocular pathogens.new approaches to antimicrobial therapy for ocular pathogens must overcome organisms that are resistant to current therapeutic modalities. this investigation examined the antimicrobial activity of novel antimicrobial neutrophil peptides (defensins np-1 and np-5) against isolates from clinical ocular microbial infections in humans and horses. the test panel of human clinical isolates included candida albicans, an alpha-hemolytic streptococcus, streptococcus pneumoniae, pseudomonas aeruginosa, and ...19902112378
in-vitro and in-vivo antibacterial activities of e1040, a new cephalosporin with potent antipseudomonal activity.e1040 is a new parenteral cephalosporin with a broad antibacterial spectrum and potent activity against gram-negative bacteria including pseudomonas aeruginosa. the in-vitro activities of e1040 against clinical isolates of enterobacter cloacae, ent. aerogenes, providencia rettgeri, and morganella morganii were superior to those of ceftazidime, cefoperazone, cefmenoxime, and cefuzonam. the activities of e1040 against gram-positive cocci were comparable with those of ceftazidime, but it was less a ...19902127928
[antimicrobial activities of gentamicin against fresh clinical isolates].antimicrobial activities of gentamicin (gm), compared with activities of other aminoglycosides (ags) and beta-lactam antibiotics, were studied against clinical isolates obtained during a period of july-december 1989. 1. gm-resistant strains were noted in 24% of staphylococcus aureus, 12% of enterobacter spp., 24% of serratia marcescens, 7% of morganella morganii and 26% of pseudomonas aeruginosa, but no gm-resistant strains were observed among isolates of escherichia coli, klebsiella pneumoniae, ...19902128340
[a rare cause of non-nosocomial pneumonia in a healthy adult: morganella morganii]. 19902147508
interactions of tazobactam and clavulanate with inducibly- and constitutively-expressed class i beta-lactamases.clavulanate and tazobactam (ytr 830) were tested as inhibitors and inducers of the ampc-type class i beta-lactamases of pseudomonas aeruginosa, enterobacter cloacae, citrobacter freundii, serratia marcescens, morganella morganii and the ic beta-lactamase of proteus vulgaris. both clavulanate and tazobactam inhibited the pr. vulgaris class ic beta-lactamase and potentiated ticarcillin and piperacillin against beta-lactamase derepressed variants of this species. tazobactam, but not clavulanate, al ...19902158496
inhibition of beta-lactamases by tazobactam and in-vitro antibacterial activity of tazobactam combined with piperacillin.the in-vitro synergistic activity of tazobactam, a new beta-lactamase inhibitor, combined with piperacillin was tested against various beta-lactamase-producing strains. the beta-lactamase inhibitory activity of tazobactam against various known types of beta-lactamase was also tested in comparison with clavulanic acid or sulbactam. tazobactam caused a remarkable reduction of the piperacillin mics for penicillinase- and oxyiminocephalosporinase-producing strains and also showed a moderate synergis ...19902161820
use of a novel phosphatase test for simplified identification of species of the tribe proteeae.phosphatase activity of 334 isolates of the tribe proteeae carefully identified to species level has been evaluated by using both a number of conventional tests and three versions of the novel methyl green-phenolphthalein (mgp) method (g. satta. r. pompei, g. grazi, and g. cornaglia, j. clin. microbiol. 26:2637-2641, 1988). we found that the different species of proteeae show different and easily distinguishable. behaviors by the mgp method, while all of them behave in a uniform way in the conve ...19902166077
aztreonam susceptibility testing. a retrospective analysis.aztreonam is a structurally and immunologically unique beta-lactam antibiotic with activity exclusively against aerobic gram-negative micro-organisms. between 1983 and 1988, its antimicrobial spectrum was evaluated against more than 5,800 fresh clinical isolates at a 300-bed community teaching hospital. only 1.1 percent of enterobacteriaceae isolates were resistant to aztreonam over the five-year study period, an incidence similar to that observed with aminoglycoside antibiotics. aztreonam was f ...19902180295
confirmation of morganella distinction from proteus and providencia among enterobacteriaceae on the basis of cellular and lipopolysaccharide fatty acid composition. 19902191036
laboratory criteria in the diagnostics of intestinal infections of staphylococcal etiology in children.faecal samples were investigated from 948 children hospitalized with the diagnose of acute intestinal infection. the authors studied the isolation and properties of a broad spectrum of microorganisms from children's faeces: salmonella, shigella, e. coli, s. aureus, rotaviruses, klebsiella, morganella, proteus. as a result, laboratory criteria were formulated of diagnosing in children intestinal infection of staphylococcal etiology.19902191037
molecular characterization of cytotoxins produced by haemophilus, actinobacillus, pasteurella.the leukotoxin of pasteurella haemolytica has been implicated as one of the major virulence factors contributing to the pathogenesis of bovine pneumonic pasteurellosis. to gain a better understanding of the various biochemical, antigenic and toxigenic properties of the toxin, the genetic determinant (lkt) which encodes the leukotoxin has been cloned from p haemolytica serotype 1. results from the molecular characterization of this determinant showed that four genes are required to synthesize and ...19902193701
characterization of enterobacteria by esterase specific-activity profiles.the spectrum of specific activities and the electrophoretic mobilities of esterases produced by 550 strains of enterobacteriaceae belonging to 36 species and subclassified into six groups (group 1, escherichia coli, shigella and escherichia hermanii; group 2, genus salmonella and genus citrobacter; group 3, genus klebsiella and genus enterobacter; group 4, genus serratia and serratia fonticola; group 5, genus proteus, genus providencia and genus morganella; and group 6, genus yersinia) were anal ...19902202780
in vitro activity of temafloxacin hydrochloride (ta-167 or a-62254), a new fluorinated 4-quinolone.the in vitro antibacterial activity of temafloxacin hydrochloride (ta-167 or a-62254) was evaluated against a wide variety of clinical isolates and compared with those of other fluoroquinolones. the potency (mic90) of the compound against gram-positive aerobic bacteria was higher or comparable to those of ciprofloxacin, ofloxacin, and norfloxacin. against most gram-negative enteric bacteria and pseudomonas species, temafloxacin was less active than ciprofloxacin, but was generally as active as o ...19902209168
infection with bacterial enteropathogens in swedish travellers to south-east asia--a prospective study.infection with potential bacterial enteropathogens was studied prospectively in 94 swedish travellers. three faecal samples were collected, regardless of diarrhoeal symptoms, after each of three 1-week stays in singapore, hong kong and japan. the specimens were analyzed for salmonella, shigella, yersinia, campylobacter and different enterotoxin-producing bacteria. a potential enteropathogen was identified in 30% (28/94) of the participants, i.e. in 26% of the healthy and in 39% of the travellers ...19902209737
use of urea filter paper disc to detect urease activity in enterobacteriaceae by multipoint replication techniques.a new method of detecting urease activity in enterobacteriaceae was developed. an 8.5 cm filter paper disc impregnated with 20% urea and 0.5% bromocresol purple was placed on the surface of a glucose fermentation plate after inoculation with a multipoint replicator and overnight incubation. this method was compared with the commercially prepared mast urea agar (multipoint) and fuscoe's urea plate medium. a total of 240 routine isolates of enterobacteriaceae were tested for urease activity using ...19902212071
numerical analysis of electrophoretic protein patterns of morganella morganii strains from faeces, wound, urine and other clinical sources.sixty-five strains of morganella morganii (mainly of human origin) were characterized by one-dimensional sds-page of cellular proteins. the strains came from various countries; 13 were from stools (including one from a toucan), 13 from wounds, 11 from urine, five from blood (including one from a snake), five from the respiratory tract (four sputum, one lung), 12 from miscellaneous sources and six from unknown sources. the protein patterns, which contained 45 to 50 discrete bands, were highly rep ...19902246146
functional similarity between the haemolysins of escherichia coli and morganella morganii.haemolysin produced by a clinical isolate of morganella morganii was examined for antigenic relatedness to the haemolysin of escherichia coli and for similarities in mode of action. the m. morganii haemolysin migrated in sds-page as a single protein band with a slightly higher molecular weight than that of e. coli haemolysin. several murine monoclonal antibodies against e. coli haemolysin cross-reacted with the m. morganii haemolysin in western blots. diminished haemolysis in the presence of osm ...19902250286
[bacterial flora of the oral cavity, fangs and venom of bothrops jararaca: possible source of infection at the site of bite].culture of fang, fang sheath and venom of fifteen healthy freshly captured bothrops jararaca were analyzed. the bacteria most frequently encountered were group d streptococci (12 snakes), enterobacter sp. (6), providencia rettgeri (6), providencia sp. (4), escherichia coli (4), morganella morganii (3) and clostridium sp. (5). the bacteria observed are similar to those found in the abscesses from bothrops bitten patients. since these snake mouth bacteria may be inoculated during the snake bite, b ...19902259834
[basic and clinical studies on norfloxacin in the pediatric field].pharmacokinetic , bacteriological and clinical studies on norfloxacin (nflx), a quinolone-carboxylic acid antibacterial agent, were conducted in the pediatric field. 1. serum concentrations and urinary excretion of nflx after single dose of 2.2 approximately 5.6 mg/kg (mean 4.4 +/- 1.2 mg/kg) were determined in 13 children with ages between 6 and 11 years. the mean peak serum concentration of the drug was 0.37 +/- 0.20 micrograms/ml at 2 hours after administration. the mean half-life of the drug ...19902262968
[fournier's disease: a report of 9 cases].nine cases of fournier's gangrene diagnosed between 1982 and 1989 are reported. all were males with a mean age of 76 (47-82 years). seven had a history of alcoholism and one had non insulin-dependent diabetes. six patients also had an anal fistula which may have been the starting point of the infection. the causal agents were two anaerobes (clostridium perfringens and bacteroides fragilis) two gram-negatives (morganella morgagni and pseudomonas aeruginosa) and one, an unidentified gram-positive. ...19902278737
in vitro activity of hre 664, a penem antibiotic.hre 664, a new penem antibiotic, inhibited 90% of escherichia coli, klebsiella, citrobacter diversus, proteus mirabilis, proteus vulgaris, salmonella, shigella, providencia, aeromonas, and morganella at less than or equal to 2 micrograms/ml but was considerably less active than cefotaxime, ceftazidime, and imipenem. it did not inhibit pseudomonas aeruginosa (mic greater than 128 micrograms/ml). hre 664 inhibited enterobacter spp., citrobacter freundii, and serratia marcescens at 1-8 micrograms/m ...19902279381
the role of microorganisms in psoriasis.the microflora of 297 psoriasis patients was extensively examined. throat, urine, and skin surfaces from scalp, ears, chest, face, axillary, submammary, umbilical, upper back, inguinal crease, gluteal-fold, perirectal, vaginal, pubis, penis, scrotal, leg, hands, feet, finger, and toenail areas were cultured for aerobic bacteria, yeast, and dermatophytes. antibody levels to streptococcal enzymes were performed (streptolysin-o, dnase-b, hyaluronidase, streptozyme). giemsa smears and koh preparatio ...19902285571
[susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates].this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ...19902287060
[clinical and diagnostic aspects of infective nephrobronchial fistulae].after reviewing all of our hospital admittances, this paper presents two cases of nephrobronchial fistula (nbf) occurring in two patients, one with renal abscess-like granulomatous pathology and one with xantogranulomatous pyelonephritis (xgp). after commenting on these cases and including iconography, among which a fistulography, very rare in this type of cases, should be emphasized, the coincidence of the three microorganisms (and the presence of morganella morganii) in lung cultures, and kidn ...19902288248
in vitro activity against clinically important gram-positive and gram-negative bacteria of sulbactam, alone and in combination with ampicillin, cefotaxime, mezlocillin, and piperacillin.the in vitro effects of the single agents, and the synergistic/antagonistic action of three different combinations of ampicillin (amp, cas 69-53-4), cefotaxime (ctx, cas 63527-52-6), mezlocillin (mez, cas 51481-65-3), and piperacillin (pip, cas 61477-96-1) with the beta-lactamase inhibitor sulbactam (sul, cas 68373-14-8) were determined against 675 gram-positive and gram-negative, both aerobic and anaerobic bacteria. all the combinations of sulbactam and the antibiotics (1: 1, 1:2 and 1:4) exhib ...19902291754
in-vitro activity of cefpodoxime against 1834 isolates from domiciliary infections at 20 uk centres.a total of 1834 non-copy, general practice or outpatient isolates were collected by 20 hospital laboratories within the british isles, and identified and tested for susceptibility to nine antimicrobial agents available by oral administration at one centre. against enterobacteriaceae cefpodoxime was the most active beta-lactam agent tested, the mic90s being: for escherichia coli 1.0 mg/l, for proteus mirabilis 1.0 mg/l, for citrobacter spp. 2.0 mg/l, and for enterobacter spp., serratia spp., morg ...19902292533
evolution of aromatic amino acid biosynthesis and application to the fine-tuned phylogenetic positioning of enteric bacteria.a comprehensive phylogenetic tree for virtually the entire assemblage of enteric bacteria is presented. character states of aromatic amino acid biosynthesis are used as criteria, and the results are compared with partial trees based upon sequencing of 16s rrna, 5s rrna, and tryptophan leader peptide. three major clusters are apparent. enterocluster 1 possesses a gene fusion (trpg-trpd) encoding anthranilate synthase: anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase of tryptop ...19902298692
purification and n-terminal analysis of urease from helicobacter pylori.urease of helicobacter pylori (formerly campylobacter pylori) is believed to represent a critical virulence determinant for this species. ammonia generated by hydrolysis of urea may protect the acid-sensitive bacterium as it colonizes human gastric mucosa. an h. pylori strain, cultured from a gastric biopsy of a patient with complaints of abdominal pain and a history of peptic ulcer disease, was isolated on selective medium and cultured in mueller-hinton broth supplemented with 4% fetal calf ser ...19902318539
diverse potential of beta-lactamase inhibitors to induce class i enzymes.the ability of various beta-lactamase inhibitors to induce class i beta-lactamases was assessed. clavulanate was the most active compound, inducing morganella morganii, aeromonas caviae, and enterobacter aerogenes over a broad concentration range and citrobacter freundii, pseudomonas aeruginosa, and serratia marcescens at high concentrations. disk approximation tests paralleled these results, with clavulanate, but not sulbactam or tazobactam, antagonizing the activity of several beta-lactams aga ...19902327752
the in-vitro activity of ceftibuten against 475 clinical isolates of gram-negative bacilli, compared with cefuroxime and cefadroxil.the in-vitro activity of ceftibuten was compared with cefuroxime and cefadroxil against 475 clinically-significant, epidemiologically-distinct isolates of gram-negative bacilli: 170 from blood, 212 from urine and 93 from a supplementary collection of multiply-resistant strains known to have resistance plasmids, to have caused sporadic or epidemic nosocomial infection, or both. ceftibuten mics ranged from 0.003 to greater than 32 mg/l, with a modal mic of 0.01 mg/l: 95% of all isolates had ceftib ...19902329100
morganella morganii urease: purification, characterization, and isolation of gene sequences.morganella morganii, a very common cause of catheter-associated bacteriuria, was previously classified with the genus proteus on the basis of urease production. m. morganii constitutively synthesizes a urease distinct from that of other uropathogens. the enzyme, purified 175-fold by passage through deae-sepharose, phenyl-sepharose, mono-q, and superose 6 chromatography resins, was found to have a native molecular size of 590 kilodaltons and was composed of three distinct subunits with apparent m ...19902345135
direct introduction of a formamido group into the 7 alpha (6 alpha)-position of cephalosporins (penicillins).a novel direct introduction of a formamido group into the 7 alpha (6 alpha)-position of cephalosporins (penicillins) was achieved by treatment of 7 beta (6 beta)-[(3,5-di-tert-butyl-4-oxo-2,5-cyclohexadien-1- ylidene)methylimino]cephem (penam) esters with n,n-bis(trimethylsilyl)formamide, followed by deblocking with girard reagent t to give the corresponding 7 alpha (6 alpha)-formamido-7 beta (6 beta)-amino derivatives. three 7 alpha-formamidocephalosporins were prepared by the conventional n-ac ...19902387776
hlyb-dependent secretion of hemolysin by uropathogenic escherichia coli requires conserved sequences flanking the chromosomal hly determinant.the synthesis and secretion of hemolysin (hlya) by escherichia coli are governed by four contiguous genes (hlycabd) that are closely conserved on plasmids and, among human pathogenic strains, on the chromosome. we have previously shown that in plasmid phly152 the coexpressed synthesis and export functions are uncoupled by intraoperon transcription termination, which is in turn alleviated by antitermination dictated in cis by a region upstream of the hly operon. in this study we describe an analo ...19901689714
[the etiological structure of acute intestinal infections].the share of various etiologic forms of acute intestinal infections, diagnosed by bacteriologic methods, is presented. the share of gastroenterocolitis induced by opportunistic microflora makes up 35.6%, that of dysentery 25.6%, salmonellosis 18.5%; mixed infection (dysentery + salmonellosis) is diagnosed in 7% of cases with acute intestinal infections. the principal representative of opportunistic microflora isolated from patients with acute intestinal infections is the klebsiella genus (40.6%) ...19901692367
protein profile typing--a new method of typing morganella morganii strains.a new, simple and stable method for typing morganella morganii strains is described. the 150 strains examined, principally from faeces, contained haemolytic and non-haemolytic representatives of diverse o serogroup, bacteriocin type and biotype. among the biotypes were some trehalose-fermenting, tetracycline-resistant strains and some non-motile, tetracycline-sensitive, glycerol fermenters. after analysis of cell lysates by sodium dodecyl sulphate-discontinuous polyacrylamide gel electrophoresis ...19901701835
new o antigens of morganella morganii and the relationships between haemolysin production. o antigens and morganocin types of strains.a collection of 142 strains of morganella morganii, principally from unrelated patients' faeces was examined to determine the relationship, if any, between haemolysin production, o antigen and morganocin production/sensitivity type. only 55 (38.7%) were agglutinable with the existing 44 o antisera. however, when o antisera were raised to some of the non-typable strains 11 new o antigens were found and 126 (88.7%) of the strains were typable. the number of o antigenic groups in m. morganii is now ...19901711754
evolution of the ferric enterobactin receptor in gram-negative bacteria.using sodium dodecyl sulfate-polyacrylamide gel electrophoresis of iron-deficient and replete cell envelopes, 59fe-siderophore uptake studies, and western immunoblots and cytofluorimetric analyses with monoclonal antibodies (mabs), we surveyed a panel of gram-negative bacteria to identify outer membrane proteins that are structurally related to the escherichia coli k-12 ferric enterobactin receptor, fepa. antibodies within the panel identified fepa epitopes that are conserved among the majority ...19911717434
microbiological evaluation of cefpodoxime proxetil.cefpodoxime, the active de-esterified molecule of the orally absorbable cephalosporin cefpodoxime proxetil, inhibits streptococci, neisseria spp., and most enterobacteriaceae, with mic50 and/or mic90 values of less than or equal to 2 mg/l; with regard to the latter family of bacteria, the mic50 and/or mic90 values of cefpodoxime are consistently greater than or equal to 4 mg/l for only enterobacter cloacae, citrobacter freundii, serratia marcescens, and morganella morganii. the mic50 of cedpodox ...19911726210
properties of a rat monoclonal antibody reactive with both the mannan of candida species and the o-antigen 6,7 polysaccharide of serogroup c1 salmonellae.monoclonal antibody masc1-mr9 of isotype immunoglobulin m was generated in lou/c rats by immunization with heat-killed salmonella thompson (serogroup c1, o-antigen 6,7 lipopolysaccharide) bacteria which had been further enriched for o-antigen by coating with homologous lipopolysaccharide. eight monoclonal antibodies were selected by screening against the lipopolysaccharide of s. thompson. in a subsequent test against candida mannan, only one antibody, masc1-mr9, was reactive. immunofluorescence ...19911702760
in-vitro evaluation of ampicillin/brobactam and comparison with other beta-lactam antibiotics.the ability of brobactam to inhibit beta-lactamases and the in vitro activity of ampicillin combined with brobactam was compared with another beta-lactamase inhibitor, clavulanic acid, and other beta-lactam antibiotics. both inhibitors showed good and similar activity against staphylococcal penicillinase and most broad-spectrum beta-lactamases found in the enterobacteriaceae, whether plasmid or chromosomally mediated. both inhibitors were less active against chromosomally mediated cephalosporina ...19911646779
in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin).the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ...19911664418
[urinary tract infection in an ambulatory population: epidemiological analysis of the etiology and antibiotic resistance of isolated gram-negative strains].15,892 urinocultures belonging to out-patients admitted to chieti ulss 04, from january '85 to december '89 were studied. among the examined samples, the positive urinocultures were 4255 (26.8%) with a prevalence in the female sex (78.6%). during the year e. coli was the most frequently identified organism (55.8%) without significant changes. 25.7% was the isolation percentage of morganella-proteus-providencia (mpp). in order to plan a right antibiotic-therapy the resistance of isolates to 19 ch ...19911670056
antimicrobial activities of piperacillin alone and in combination with tazobactam against beta-lactamase-producing bacteria.tazobactam (ytr 830), a new beta-lactamase inhibitor, was evaluated for its effect in combination with piperacillin, a broad spectrum, but beta-lactamase sensitive, penicillin, against 14 common bacteria. a total of 1,086 clinical isolates from different clinical specimens were tested for beta-lactamase production by the rapid chromogenic cephalosporin method. their susceptibilities to piperacillin alone and in combination with tazobactam in a ratio of 8:1 by the agar dilution method were evalua ...19911685175
production and purification of l-phenylalanine oxidase from morganella morganii.an enzyme fraction, acting predominantly on l-phenylalanine has been purified and characterized from morganella morganii. the total envelope was prepared by disrupting the cells with a french press followed by high speed centrifugation. after solubilization of the particulate fraction with 0.1% triton x-100 and then centrifugation, the resulting supernatant was layered onto a deae-cellulose column. active fractions eluted were applied to a phenyl-sepharose cl-4b column as the final purification ...19911368081
in vitro activity of the novel cephalosporin gr69153.the in vitro activity of gr69153 was compared to that of ceftazidime, ceftriaxone, imipenem and gentamicin against a total of 702 recent clinical isolates. mics were determined by a standard agar dilution procedure and two inocula (10(4) and 10(8) cfu) were used throughout. gr69153 inhibited 90% of isolates of escherichia coli, klebsiella pneumonia and proteus mirabilis at less than or equal to 0.25 mg/l and 90% of isolates of pseudomonas aeruginosa at less than or equal to 1 mg/l. citrobacter f ...19911748126
comparative study of pharmacokinetics and serum bactericidal activity of ceftizoxime and cefotaxime.single 2-g intravenous doses of ceftizoxime (czx) and cefotaxime (ctx) were given over 30 min to 10 adult volunteers in a crossover manner on two separate occasions. concentrations of czx, ctx, and the primary metabolite of ctx, desacetylcefotaxime (dctx), in serum, suction-induced-blister fluid, and urine were determined by high-pressure liquid chromatography. pharmacokinetic parameters were estimated by using an extended least-squares modeling program (mkmodel). czx exhibited a half-life in se ...19911759827
evaluation of brl 42715, a beta-lactamase-inhibiting penem.brl 42715 is a new penem which inhibits a wide range of plasmid as well as chromosomally mediated bacterial beta-lactamases. we used a total of 902 recent clinical isolates, consisting of 455 enterobacteriaceae, 247 staphylococci and 200 other gram-negative bacteria to evaluate its ability for potentiation of amoxycillin. mics for all the 104 strains of methicillin-susceptible staphylococcus aureus were reduced from 8-greater than 32.0 to less than or equal to 0.06 micrograms/ml in the presence ...19911760938
a new plate medium for rapid presumptive identification and differentiation of enterobacteriaceae.a new selective differential agar medium for rapid presumptive identification of enterobacteriaceae from water and food samples is described (emx id agar). by a combination of fluorogenic and chromogenic substrates, the medium detects the presence of beta-d-glucuronidase, beta-d-galactosidase, beta-d-xylosidase, tryptophane deaminase and h2s; additionally, cytochrome-oxidase and indole production can be demonstrated. this medium provides an inexpensive means for simple and rapid presumptive iden ...19911777382
beta-lactamase expression and outer membrane protein changes in cefpirome-resistant and ceftazidime-resistant gram-negative bacteria.twenty-five strains of enterobacteriaceae (five each of enterobacter cloacae, citrobacter freundii, serratia marcescens, morganella morganii, and providencia stuartii) and five strains of pseudomonas aeruginosa were exposed to various concentrations of cefpirome or ceftazidime in agar. mutants with a greater than four-fold increase in the mic were examined for changes in beta-lactamase expression and outer membrane protein (omp) profile. both agents selected mutants with decreased susceptibility ...19911778852
selection and characterization of cefepime-resistant gram-negative bacteria.the nctc type strains and four clinical isolates of enterobacter cloacae, citrobacter freundii, serratia marcescens, morganella morganii, providencia stuartii and pseudomonas aeruginosa were exposed, in agar, to cefepime at 3, 5, and 10 x mic and a breakpoint concentration of 16 mg/l. mutants were selected at a frequency of approximately 10(-8) that had decreased susceptibility to cefepime and cefpirome, and species-dependent resistance to other beta-lactams. any putative mutant with a greater t ...19911778870
in vitro activity of a new cephalosporin me-1206 compared with other agents.the in vitro activity of me-1206, a new aminothiazolyl cephalosporin that can be orally absorbed when converted to an ester, was compared with that of other beta-lactams. me-1206 inhibited 50% of the enterobacteriaceae at 2 micrograms/ml, similar to cefotaxime, ceftazidime, and cefixime. it did not inhibit, mic greater than or equal to 32 micrograms/ml, enterobacter species or citrobacter freundii resistant to cefotaxime and ceftazidime, and it was less active than cefotaxime and ceftazidime aga ...19911797456
in vitro activity evaluations of cefdinir (fk482, ci-983, and pd134393). a novel orally administered cephalosporin.cefdinir, a so-called third-generation oral cephalosporin was tested in vitro against over 700 pathogens from patients with bacteremia. cefdinir was very active against the enterobacteriaceae with a 50% minimum inhibitory concentration (mic50) value range of less than or equal to 0.03-8 micrograms/ml. the enteric species having the highest mic90s (greater than or equal to 16 micrograms/ml) were citrobacter freundii, and the enterobacters, morganella morganii, proteus vulgaris, and serratia marce ...19911797457
[susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates].we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ...19911798067
antibacterial activity of cefpodoxime in comparison with cefixime, cefdinir, cefetamet, ceftibuten, loracarbef, cefprozil, bay 3522, cefuroxime, cefaclor and cefadroxil.the new oral cephalosporins cefpodoxime, cefixime, cefdinir, cefetamet and ceftibuten demonstrate enhanced activity against enterobacteriaceae susceptible to the established compounds as well (e.g. cefuroxime, cefaclor, cefadroxil). in addition, cefpodoxime, cefixime, cefdinir, cefetamet and ceftibuten include in their spectrum species hitherto resistant to oral cephalosporins (proteus vulgaris, providencia spp., yersinia enterocolitica). besides, the majority of these compounds demonstrate rele ...19911800377
cefpodoxime: comparative antibacterial activity, influence of growth conditions, and bactericidal activity.the antimicrobial activity of cefpodoxime, the active metabolite of the new cephalosporin ester cefpodoxime proxetil, in comparison to cefixime, cefotiam, cefuroxime, and cefotaxime was determined against a broad spectrum of freshly isolated gram-positive and gram-negative bacterial strains. cefpodoxime was demonstrated to be inhibitory at concentrations of less than or equal to 1 mg/l against 90% of strains of moraxella catarrhalis, haemophilus influenzae, escherichia coli (beta-lactamase- nega ...19911800379
activity of meropenem against antibiotic-resistant or infrequently encountered gram-negative bacilli.meropenem was compared in vitro with imipenem as well as with several other contemporary beta-lactams, ciprofloxacin, and gentamicin against a group of highly antibiotic resistant members of the family enterobacteriaceae and a collection of oxidase-positive and/or glucose-nonfermentative gram-negative bacilli. in this study, meropenem was more active than imipenem against isolates of enterobacter, klebsiella, morganella, providencia, alcaligenes, aeromonas, and pasteurella.19911804016
use of peyer's patch and lymph node fragment cultures to compare local immune responses to morganella morganii.lymphoid tissue fragment cultures were established to analyze the differentiative processes among b cells in peyer's patches (pp) and peripheral lymph nodes (pln), especially those in germinal centers. pp cultures from both conventionally reared mice and formerly germ-free mice colonized with morganella morganii could be maintained for greater than 12 days with continued b-cell division, especially among cells binding high levels of peanut agglutinin, a characteristic of germinal center cells. p ...19911825484
topoisomerase mutants and physiological conditions control supercoiling and z-dna formation in vivo.the influence of topoisomerase i and gyrase mutations in escherichia coli on the supercoiled density of recombinant plasmids and the stability of left-handed z-dna was investigated. the formation of z-dna in vivo by dc-dg sequences of different lengths was used to determine the effective plasmid supercoil densities in the mutant strains. the presence of z-dna in the cells was detected by linking number and ecori methylase inhibition assays. a change in the unrestrained superhelical tension in vi ...19911846630
a conserved domain on enterobacterial porin protein analysed by monoclonal antibody.broadly cross-reactive monoclonal antibodies (mabs) against enterobacterial outer membrane (om) porin (po) protein were isolated after immunization of balb/c mice with whole cells of e. coli 055:b5. mabs (n = 6) of the igg class but of four different isotypes were studied. based on a competition elisa, all of the mabs were directed against one and the same po protein domain (po i). the mabs cross-reacted with 72 of 74 strains from 10 different genera of the enterobacteriaceae. one morganella and ...19911847063
in vitro evaluation of gr69153, a novel catechol-substituted cephalosporin.gr69153 is a c-7 catechol cephalosporin with a broad spectrum of activity against members of the family enterobacteriaceae (mics for 50% of strains tested [mic50s], 0.008 to 0.5 micrograms/ml), staphylococcus aureus (mic50, 4 micrograms/ml), pseudomonas aeruginosa (mic50, 0.25 micrograms/ml), haemophilus influenzae (mic50, 0.03 micrograms/ml), neisseria gonorrhoeae (mic50, 0.03 micrograms/ml), and acinetobacter spp. (mic50, 2 micrograms/ml). potent gr69153 activity was also demonstrated against ...19911854174
mode of antibacterial action of cefprozil, a new cephalosporin, on escherichia coli, serratia marcescens and morganella morganii.the mode of antibacterial action of cefprozil (cfpz, bmy-28100), a newly developed cephalosporin, was investigated using escherichia coli k12, serratia marcescens ifo 12648 and morganella morganii ifo 3848 as test organisms, in comparison with the action of cefaclor (ccl). the minimum inhibitory concentrations (mics) of cfpz for these organisms were 1.56, 800 and 25 micrograms/ml, whereas those of ccl were 1.56, 800 and 100 micrograms/ml, respectively. the addition of a subinhibitory concentrati ...19911864765
in vitro and in vivo evaluation of ro 09-1428, a new parenteral cephalosporin with high antipseudomonal activity.ro 09-1428, a new parenteral cephalosporin with a catechol moiety attached at position 7 of the cephalosporin ring, showed high in vitro activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, and streptococcus pyogenes, with mics for 90% of strains tested (mic90s) of less than or equal to 0.39 micrograms/ml. morganella morganii, providencia rettgeri, citrobacter freundii, haemophilus influenzae, staphylococcus aureus, and streptococcus pneumoniae were inhi ...19911906261
catheter-associated urinary tract infections: epidemiology, pathogenesis, and prevention.catheter-associated urinary tract infections (utic) remain the most common nosocomial infection. although usually benign, utic cause bacteremia in 2-4% of patients and have been associated with a case fatality rate three times as high as nonbacteriuric patients. risk factors for utic identified in multivariate analyses include increasing duration of use, female sex, absence of systemic antibiotics, and disconnection of the catheter-collecting tube junction. recent studies suggest that most episo ...19911928194
in vitro antibacterial activity of me1207, a new oral cephalosporin.me1207 is the prodrug of me1206. its in vitro antibacterial activity was compared with that of cefteram, cefpodoxime, cefixime, and cefaclor against various clinical isolates. me1206 was more active than the other cephems tested against staphylococci, streptococci, morganella morganii, pseudomonas cepacia, and flavobacterium meningosepticum and had the most potent activity against haemophilus influenzae and neiserria gonorrhoeae. the drug also showed a wide spectrum of activity against other gra ...19911929344
incidence of multi-resistance in gram-negative aerobes from intensive care units of 10 german hospitals.at the beginning of 1990, 10 german hospitals participated in a surveillance study of 100 consecutively submitted gram-negative aerobes originating in their intensive care units (icus). a total of 1,006 isolates were obtained from 446 patients. resistance rates cited apply to the initial strains isolated and use the nccls fully susceptible breakpoint. over 1/3 of the penicillin resistant populations of e. coli and klebsiella spp. were resistant to amoxicillin/clavulanate and simultaneously resis ...19911947820
enterotoxin-producing bacteria isolated from swedish travellers with diarrhoea.the isolation rate of bacterial enteropathogens of different species, particularly enterotoxin-producing gram-negative bacteria, was determined in stool specimens from swedish travellers with diarrhoea. overall, bacterial enteropathogens were identified in 101 (47%) of the 217 travellers on their return home. the most common isolates were enterotoxin-producing bacteria (20%), salmonellae (18%) and campylobacter (8%), whereas shigellae (3%) and yersinia (0.5%) were rarely identified. mixed infect ...19911957131
characterization of fce 22101-resistant enterobacteriaceae and the effect of fce 22101 upon the activity of anti-pseudomonal beta-lactams for pseudomonas aeruginosa.twenty-five strains of enterobacteriaceae (five each of enterobacter cloacae, citrobacter freundii, serratia marcescens, morganella morganii, and providencia stuartii) were exposed to fce 22101 in agar containing 3, 5, or 10 x the mic. any putative mutant with a greater than or equal to four-fold increase in the mic was examined for beta-lactamase expression and outer membrane protein (omp) profile. mutant colonies were selected at a frequency of 10(-7)-10(-11) with decreased susceptibility to f ...19911960118
[antibiotic sensitivity of bacteria isolated from patients at 6 hospitals in osaka].we examined mics of 6 oral new quinolones and 4 oral cephems against bacteria isolated from patients at 6 hospitals in osaka during the period from january to june in 1990, and the following results were obtained. 1. all species excluding streptococcus pyogenes were more sensitive with less frequencies of resistance to new quinolones than to cephems. in new quinolones, tosufloxacin (tflx) was the most active against gram-positive cocci and ciprofloxacin (cpfx) and tflx had the highest activities ...19911960855
[antibacterial activities of ofloxacin against recent isolates from patients with community-acquired infections].in order to survey antibacterial activities of ofloxacin (oflx) against 1,440 bacterial strains isolated from patients with community-acquired infections in 1987 and 1990, minimum inhibitory concentrations (mics) of the drug as well as those of other new quinolones and oral cephems were determined. the following conclusions were reached. 1. comparison of the mic distribution for strains isolated in 1987 with those in 1990 suggested a tendency toward an increase in the frequency of oflx-resistant ...19911960856
role of bacterial association with kupffer cells in occurrence of endogenous systemic bacteremia.bacteremia in immunocompromised hosts often arises from their endogenous intestinal flora. we produced experimental endogenous bacteremia by administering cyclophosphamide and ampicillin to conventional and specific-pathogen-free mice. the frequencies of bacteremia and mortality in the conventional mice were significantly higher than for the specific-pathogen-free mice. pseudomonas aeruginosa was the major pathogen causing systemic bacteremia in conventional mice and was associated with a high m ...19911987043
pyridoxal phosphate-dependent histidine decarboxylases. cloning, sequencing, and expression of genes from klebsiella planticola and enterobacter aerogenes and properties of the overexpressed enzymes.the hdc genes encoding the inducible pyridoxal-p-dependent histidine decarboxylase (hisdcase) of klebsiella planticola and enterobacter aerogenes were isolated, sequenced, and expressed in escherichia coli under control of the lac promoter, and the overproduced enzymes were purified to homogeneity from the recombinant host. formation of inclusion bodies during synthesis of the e. aerogenes enzyme was avoided by cooling the culture and inducing at 25 degrees c. the cloned enzymes were produced in ...19912033044
preferential hydrolysis of cis configuration compounds at the 3,4 position of monobactams by beta-lactamase from morganella morganii.carumonam and bo-1166 (cis configuration) were inactivated by beta-lactamase of morganella morganii more rapidly than were aztreonam and bo-1165 (trans configuration), as demonstrated by spectrophotometric analysis and microbiological assay. an active enzyme was recovered more rapidly from the inactivated enzyme-monobactam complex derived from the cis form of monobactams than from the complex derived from the trans form of monobactams. this result suggests that the configuration at the 3,4 posit ...19912039196
[antibacterial activity of ciprofloxacin against fresh clinical isolates from superficial suppurative foci].the minimum inhibitory concentrations (mics) of 5 drugs (ciprofloxacin (cpfx), and 4 drugs used as standard) were determined to investigate antibacterial potencies of cpfx against bacterial strains isolated in 1989 from superficial suppurative foci. the clinical isolates tested included 375 strains from 11 aerobic bacterial species, and 50 strains from 2 anerobic bacterial genera (group) for a total of 425 isolates. interpreting mic level distributions of these drugs as the expression of antibac ...19912041153
[antimicrobial susceptibilities of clinical isolates of morganella-proteus-providencia group of bacteria].we examined in vitro susceptibilities of 3,109 isolates belonging to 5 species of proteeae to 2 penicillins, 5 cephems and 2 aminoglycosides. the isolates were collected from 69 hospital laboratories throughout japan between 1986 and 1988. minimal inhibitory concentrations were determined using the agar dilution method with inoculation of 10(8) cells/ml of bacteria. proteus mirabilis had marked susceptibilities to the penicillins and cephems tested. proteus vulgaris and morganella morganii were ...19912041155
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