mortality due to nosocomial infection with klebsiella pneumoniae esbl().klebsiella pneumoniae is one of the most important hospital pathogens, particularly concerning the multidrug-resistant strain esbl(+). the aim of this study was to evaluate nosocomial infections with k. pneumoniae esbl(+) in the context of infection location, risk factors, and prognosis. this hospital study was conducted retrospectively and covered a 3 months' period. the infection with k. pneumoniae esbl(+) was diagnosed in 36 patients (19 women and 17 men) of the mean age of 74.2 ± 14.8 years. ...201728456930
long-lasting outbreak due to ctx-m-15-producing klebsiella pneumoniae st336 in a rehabilitation ward: report and literature review.whereas klebsiella species are the most frequently occurring agents in nosocomial outbreaks due to multidrug-resistant gram-negative organisms, very few outbreaks have been reported from rehabilitation wards.201728454769
characteristics of carbapenemase-producing klebsiella pneumoniae as a cause of neonatal infection in shandong, china.the goal of the present study was to examine the characteristics of carbapenem-resistant klebsiella pneumoniae as a cause of neonatal infection. a total of 37 carbapenem-resistant klebsiella pneumoniae-positive newborns hospitalized in shandong provincial hospital, china between april 2011 and october 2013 were examined. antibiotic susceptibility testing was performed using the agar dilution method and the etest. resistance genes were assessed by polymerase chain reaction (pcr) and sequencing. p ...201728450951
retrospective analysis of fosfomycin combinational therapy for sepsis caused by carbapenem-resistant klebsiella pneumoniae.the aim of the present study was to compare the efficacy and safety of fosfomycin combinational therapy with other antibiotics for the treatment of infections caused by carbapenem-resistant klebsiella pneumoniae (crkp). this retrospective cohort study examined 104 cases of sepsis caused by crkp occurring between january 2012 and november 2014 in shanghai tenth people's hospital. three categories of patient outcome were assessed: survival/mortality, duration of intensive care unit stays and durat ...201728450933
human alkaline phosphatase dephosphorylates microbial products and is elevated in preterm neonates with a history of late-onset sepsis.a host defense function for alkaline phosphatases (alps) is suggested by the contribution of intestinal alp to detoxifying bacterial lipopolysaccharide (endotoxin) in animal models in vivo and the elevation of alp activity following treatment of human cells with inflammatory stimuli in vitro. however the activity of alp in human plasma (primarily tissue-nonspecific alp; tnap) on lipopolysaccharide and other microbial products has not been assessed, nor has its expression been studied in preterm ...201728448526
clonal spread of 16s rrna methyltransferase-producing klebsiella pneumoniae st37 with high prevalence of esbls from companion animals in china.we screened 30 klebsiella pneumoniae isolates from dogs and cats at a single animal hospital in guangdong province, china. among them, 12 k. pneumoniae strains possessed high-level resistance to amikacin and gentamicin and these were screened for 16s rrna methyltransferase (16s-rmtase) genes. and then the genes positive isolates were detected for esbls (extended spectrum β-lactamases) and analyzed by pulsed-field gel electrophoresis, multilocus sequence typing, pcr-based replicon typing and plas ...201728446899
pharmacodynamics of colistin and fosfomycin: a 'treasure trove' combination combats kpc-producing klebsiella pneumoniae.kpc-producing klebsiella pneumoniae are an emerging public health problem around the globe. we defined the combinatorial pharmacodynamics and ability to suppress resistance of two 'old' antibiotics, fosfomycin and colistin, in time-kill experiments and hollow-fibre infection models (hfim).201728444224
pseudomonas aeruginosa prevalence, antibiotic resistance and antimicrobial use in chinese burn wards from 2007 to 2014.objective to assess the application of antibacterial agents, alongside pathogen prevalence and pseudomonas aeruginosa drug resistance, with the aim of understanding the impact of inappropriate antibacterial use. methods this retrospective study assessed bacteria from wounds, catheters, blood, faeces, urine and sputum of hospitalized patients in burn wards between 2007 and 2014. the intensity of use of antibacterial agents and resistance of p. aeruginosa to common anti-gram-negative antibiotics w ...201728443385
effect of appropriate combination therapy on mortality of patients with bloodstream infections due to carbapenemase-producing enterobacteriaceae (increment): a retrospective cohort study.the best available treatment against carbapenemase-producing enterobacteriaceae (cpe) is unknown. the objective of this study was to investigate the effect of appropriate therapy and of appropriate combination therapy on mortality of patients with bloodstream infections (bsis) due to cpe.201728442293
multihospital occurrence of pan-resistant klebsiella pneumoniae sequence type 147 with an isecp1-directed blaoxa-181 insertion in the mgrb gene in the united arab emirates.the emergence of pan-resistant klebsiella pneumoniae strains is an increasing concern. in the present study, we describe a cluster of 9 pan-resistant k. pneumoniae sequence type 147 (st147) isolates encountered in 4 patients over nearly 1 year in 3 hospitals of the united arab emirates (uae). the isolates exhibited highly similar genotypes. all produced chromosomally encoded oxa-181, and the majority also produced the ndm-5 carbapenemase. as with the previously described single isolate from the ...201728438945
emergence of the plasmid-mediated mcr-1 gene in clinical kpc-2-producing klebsiella pneumoniae sequence type 392 in brazil. 201728438940
emergence and evolution of multidrug-resistant klebsiella pneumoniae with both blakpc and blactx-m integrated in the chromosome.the extended-spectrum-β-lactamase (esbl)- and klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae represent serious and urgent threats to public health. in a retrospective study of multidrug-resistant k. pneumoniae, we identified three clinical isolates, cn1, cr14, and ny9, carrying both blactx-m and blakpc genes. the complete genomes of these three k. pneumoniae isolates were de novo assembled by using both short- and long-read whole-genome sequencing. in cr14 and ny9, blactx ...201728438939
in vitro assessment of combined polymyxin b and minocycline therapy against klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae.the multidrug resistance profiles of klebsiella pneumoniae carbapenemase (kpc) producers have led to increased clinical polymyxin use. combination therapy with polymyxins may improve treatment outcomes, but it is uncertain which combinations are most effective. clinical successes with intravenous minocycline-based combination treatments have been reported for infections caused by carbapenemase-producing bacteria. the objective of this study was to evaluate the in vitro activity of polymyxin b an ...201728438930
capsular polysaccharide types and virulence-related traits of epidemic kpc-producing klebsiella pneumoniae isolates in a chinese university hospital.klebsiella pneumoniae is an important human pathogen associated with a variety of diseases and the prevalence of blakpc carrying k. pneumoniae (kpc-kp) is rapidly increasing. capsule is an important virulence factor in k. pneumoniae. in this study, we determined to first systematically characterize capsular polysaccharide (cps) and virulence traits in kpc-kp strains. a total of 56 kpc-kp isolates were recovered from clinical samples in a chinese hospital, which were assigned to clonal lineages b ...201728437231
first case of ndm-1-producing klebsiella pneumoniae in annaba university hospital, algeria.the aim of this study was to characterize two carbapenem-resistant klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at annaba university hospital (algeria) in 2014.201728437228
siderophores in clinical isolates of klebsiella pneumoniae promote ciprofloxacin resistance by inhibiting the oxidative explore the relevance of and understand the potential mechanisms behind the production of siderophores by clinical isolates of k. pneumoniae and ciprofloxacin (cip) resistance, we divided the k. pneumoniae isolates into two groups based on bacterial siderophores production: high siderophore-yielding group (39 strains) and low siderophore-yielding group (38 strains). the rate of cip resistance in k. pneumoniae (27/39 = 69.23%) from the high siderophore-yielding group was significantly higher t ...201728435070
klebsiella pneumoniae carbapenemase-producing serratia marcescens outbreak in a university hospital. 201728431850
occurrence of qnrb1 and qnrb12 genes, mutation in gyra and ramr, and expression of efflux pumps in isolates of klebsiella pneumoniae carriers of blakpc-2.the occurrence of quinolone-resistance genes (qnra, qnrb and qnrs), the presence of mutations in gyra, gyrb and parc, as well as the expression of efflux pumps (acrb and acrf) and mutations in the gene ramr.201728425875
seasonal fluctuations of microbial aerosol in live poultry markets and the detection of endotoxin.microbial aerosol whose species and concentrations are closely related to human health is ubiquitous. the effect of microbes on human and animal health and production performance is, in many cases, caused by the spreading of air. samples in this experiment were from a live poultry market (lpm) in tai'an, china, collected three times a day (8 am, 14 pm, and 20 pm) over three consecutive days each month for 11 months (original sampling plan was a year, the government due to the environmental prote ...201728424676
microbiota-induced antibodies are essential for host inflammatory responsiveness to sterile and infectious stimuli.the indigenous intestinal microbiota is frequently considered an additional major organ of the human body and exerts profound immunomodulating activities. germ-free (gf) mice display a significantly different inflammatory responsiveness pattern compared with conventional (cv) mice, and this was dubbed a "hyporesponsive phenotype." taking into account that the deposition of immune complexes is a major event in acute inflammation and that gf mice have a distinct ig repertoire and b cell activity, ...201728424241
mcr-1 colistin resistance in esbl-producing klebsiella pneumoniae, france.we report intestinal carriage of an extended-spectrum β-lactamase-producing klebsiella pneumoniae strain with high-level resistance to colistin (mic 24 mg/l) in a patient in france who had been hospitalized for fungal meningitis. the strain had the mcr-1 plasmid gene and an inactivated mgrb gene, which are associated with colistin resistance.201728418313
in vitro discordance with in vivo activity: humanized exposures of ceftazidime-avibactam, aztreonam, and tigecycline alone and in combination against new delhi metallo-β-lactamase-producing klebsiella pneumoniae in a murine lung infection model.the management of infections with new delhi metallo-beta-lactamase-1 (ndm)-producing bacteria remains clinically challenging given the multidrug resistant (mdr) phenotype associated with these bacteria. despite resistance in vitro, ceftazidime-avibactam previously demonstrated in vivo activity against ndm-positive enterobacteriaceae herein, we observed in vitro synergy with ceftazidime-avibactam and aztreonam against an mdr klebsiella pneumoniae harboring ndm. in vivo, humanized doses of ceftazi ...201728416558
deciphering heteroresistance to colistin in a klebsiella pneumoniae isolate from marseille,, we report the description of a colistin-heteroresistant klebsiella pneumoniae isolate fortuitously isolated from the stool sample of a patient with suspicion of tuberculosis in a public hospital of marseille, france. in the colistin-resistant subpopulation, a mutation in the mgrb gene leading to a premature stop codon was found, and the hypermucoviscous phenotype was lost. susceptibility to other antibiotics remained unchanged. to our knowledge, this is the first identification of such a c ...201728416544
dusuqing granules (dsq) suppress inflammation in klebsiella pneumonia rat via nf-κb/mapk signaling.dusuqing granules (dsq) have been used in the treatment of bacterial pneumonia clinically, with remarkable benefits. this study was initiated to explore the effects of dsq on pulmonary inflammation by regulating nuclear factor (nf)-κb/mitogen-activated protein kinase (mapk) signaling in bacterial pneumonia rats.201728415997
inhibitor κbα protein therapy alleviates severe pneumonia through inhibition of nuclear factor κ investigate the effect of inhibitor κbα (iκbα) on severe pneumonia and explain the mechanisms of nuclear factor κb (nf-κb), the activation of nf-κb was induced in sprague-dawley (sd) rats infected with klebsiella pneumoniae (k. pneumoniae). the rats were then treated with differing concentrations of iκbα protein. a histological analysis was performed to compare the lung structure prior to and following treatment, and an immunohistochemistry assay was used to detect nf-κb activity. in addition ...201728413484
prevalence, molecular epidemiology and intra-hospital acquisition of klebsiella pneumoniae strains producing carbapenemases in an italian teaching hospital from january 2015 to september 2016.we described klebsiella pneumoniae producing carbapenemase (cpkp) spread from 01/01/2015 to 13/09/16 in a tertiary level hospital.201728412407
formulation of carbapenems loaded gold nanoparticles to combat multi-antibiotic bacterial resistance: in vitro antibacterial study.despite the fact that carbapenems (powerful β-lactams antibiotics) were able to fight serious infectious diseases, nowadays the spread of carbapenems-resistant bacteria is considered the main challenge in antibacterial therapy. in this study, we focused on evaluating the surface conjugation of carbapenems (imipenem and meropenem) with gold nanoparticles as a delivering strategy to specifically and safely maximize their therapeutic efficacy while destroying the developing resistance of the pathog ...201728411141
a molecular docking and dynamics approach to screen potent inhibitors against fosfomycin resistant enzyme in clinical klebsiella pneumoniae.klebsiella pneumoniae, ba6753 was cultured from a patient in the clinical microbiology laboratory of christian medical college. k. pneumoniae, ba6753 has a multidrug resistance plasmid encoding novel fosa variant-7, fosfomycin resistance enzyme. minimal side effects and a wide range of bactericidal activity of fosfomycin have resulted in its expanded clinical use that prompts the rise of fosfomycin-resistant strains. at present, there are no effective inhibitors available to conflict the fosa-me ...201728409871
colibactin contributes to the hypervirulence of pks(+) k1 cc23 klebsiella pneumoniae in mouse meningitis infections.klebsiella pneumoniae is the most common pathogen of community-acquired meningitis in taiwan. however, the lack of a physiologically relevant meningitis model for k. pneumoniae has impeded research into its pathogenesis mechanism. based on the core genome mlst analyses, the hypervirulent k1 k. pneumoniae strains, which are etiologically implicated in adult meningitis, mostly belong to a single clonal complex, cc23. some k1 cc23 k. pneumoniae strains carry a gene cluster responsible for colibacti ...201728409125
detection of pathogenic bioaerosols and occupational risk in a philippine landfill site.payatas landfill site is one of the major dumpsites in the philippines. in this study, the landfill was evaluated regarding the presence and concentration of pathogenic bacterial aerosols through culture and molecular techniques. bioaerosols are biological airborne particles that may or may not cause diseases. active sampling was done using mas-100 air sampler from both indoor and outdoor locations. to test the accuracy of the culture method, those that were presumptively identified as staphyloc ...201728406366
iga/igm responses to gram-negative bacteria are not associated with perinatal depression, but with physio-somatic symptoms and activation of the tryptophan catabolite pathway at the end of term and postnatal anxiety.evidence has implicated the translocation of commensal gram-negative bacteria (gram-b) due to leaky gut in the pathophysiology of depression and physio-somatic symptoms (e.g. fatigue, pain, irritable bowel syndrome, malaise, etc.). in addition, the leaky gut may contribute to immune-inflammatory activation and oxidative stress. this study investigated whether bacterial translocation is associated with perinatal depression and anxiety scores and with prenatal physio-somatic symptoms and immune-in ...201728403801
whole-genome sequencing of klebsiella pneumoniae mdr strain isolated in a syrian refugee. 201728403683
a kaempferol triglycoside from tephrosia preussii taub. (fabaceae).a phytochemical investigation of the meoh extract of twigs and leaves of tephrosia preussi was carried out to give a new kaempferol triglycoside, named tephrokaempferoside (1), together with five known compounds: tephrosin (2), betulinic acid (3), lupeol (4), β-sitosterol (5) and 3-o-β-d-glucopyranoside of β-sitosterol (6). the structure of the new compound was characterised by analyses of nmr (1d and 2d) and ms data, and chemical conversion. tephrokaempferoside (1) had weak antibacterial activi ...201728403640
hypervirulence and hypermucoviscosity: two different but complementary klebsiella spp. phenotypes?since the hypermucoviscous variants of klebsiella pneumoniae were first reported, many cases of primary liver abscesses and other invasive infections caused by this pathogen have been described worldwide. hypermucoviscosity is a phenotypic feature characterized by the formation of a viscous filament ≥5 mm when a bacterial colony is stretched by a bacteriological loop; this is the so-called positive string test. hypermucoviscosity appears to be associated with this unusual and aggressive type of ...201728402698
identification of is1r and is10r elements inserted into ompk36 porin gene of two multidrug-resistant klebsiella pneumoniae hospital klebsiella pneumoniae strains (n = 196) were collected in 2012-16 from the patients of a moscow neurosurgical intensive care unit. klebsiella pneumoniae strains were multidrug-resistant and carried beta-lactamase genes blashv (97.4% of strains), blactx-m (84.7%), blatem (56.1%), blaoxa-48-like (49.0%) and blandm-1 (one strain), class 1 integrons (43.4% of strains) and porin protein ompk36 gene (100% of strains). the ompk36 porin protein gene disruption by insertion sequence (is) element ...201728402509
imipenem/cilastatin encapsulated polymeric nanoparticles for destroying carbapenem-resistant bacterial isolates.carbapenem-resistance is an extremely growing medical threat in antibacterial therapy as the incurable resistant strains easily develop a multi-resistance action to other potent antimicrobial agents. nonetheless, the protective delivery of current antibiotics using nano-carriers opens a tremendous approach in the antimicrobial therapy, allowing the nano-formulated antibiotics to beat these health threat pathogens. herein, we encapsulated imipenem into biodegradable polymeric nanoparticles to des ...201728399890
modifiable risk factors for the spread of klebsiella pneumoniae carbapenemase-producing enterobacteriaceae among long-term acute-care hospital patients.objective to identify modifiable risk factors for acquisition of klebsiella pneumoniae carbapenemase-producing enterobacteriaceae (kpc) colonization among long-term acute-care hospital (ltach) patients. design multicenter, matched case-control study. setting four ltachs in chicago, illinois. participants each case patient included in this study had a kpc-negative rectal surveillance culture on admission followed by a kpc-positive surveillance culture later in the hospital stay. each matched cont ...201728397615
know thy self, know thy enemy: a current survey and a forecast for kpc-producing klebsiella pneumoniae resistance among inpatients in southern brazil. 201728397613
delay in human neutrophil constitutive apoptosis after infection with klebsiella pneumoniae serotype k1.klebsiella pneumoniae serotype k1 is a major cause of invasive syndrome defined by liver abscess with metastatic infections at other body sites. this culprit is known to be resistant to neutrophil phagocytosis and bactericidal activity. we hypothesized that k. pneumoniae serotype k1 might regulate neutrophil apoptosis and enhance the survival of the infected neutrophils that might serve as a vector for dissemination of the bacteria. two serotypes of k. pneumoniae, kp-m1 isolated from a patient w ...201728396849
highly tigecycline-resistant klebsiella pneumoniae sequence type 11 (st11) and st147 isolates from companion this study, we characterized two tigecycline-resistant klebsiella pneumoniae isolates from dog urine samples. the isolates were genetically unrelated, belonging to sequence type 11 (st11) and st147, both classically related to human isolates. to the best of our knowledge, this is the first identification of tigecycline-resistant isolates from animals. we unveil here the worrisome circulation among animals of bacterial clones resistant to this last-resort antibiotic.201728396550
resistance to ceftazidime-avibactam in klebsiella pneumoniae due to porin mutations and the increased expression of kpc-3. 201728396547
lipocalin-2 from both myeloid cells and the epithelium combats klebsiella pneumoniae lung infection in mice. 201728396494
dissemination and clinical implications of multidrug-resistant klebsiella pneumoniae isolates producing oxa-48 in a spanish infections caused by klebsiella pneumoniae isolates are increasing and few effective antibiotics are currently available to treat patients.201728395861
genome sequencing and comparative analysis of an ndm-1-producing klebsiella pneumoniae st15 isolated from a refugee patient.the escalating problem of antibiotic resistance, specifically cabarpenemase and extended-spectrum β-lacatamase (esbl) producing k. pneumoniae strains, is directly correlated with increased patient morbidity and mortality and prolonged hospitalization and costs. in this study, a comprehensive genomic analysis encompassing the resistomics, virulence repertoire and mobile genetic elements of an ndm-1 positive esbl-producing k. pneumoniae ea-meh st15 isolated from a urine sample collected from a syr ...201728395597
first report of invasive liver abscess syndrome with endophthalmitis caused by a k2 serotype st2398 hypervirulent klebsiella pneumoniae in germany, 2016.we report a case of severe infection with liver abscess and endophthalmitis caused by a hypervirulent klebsiella pneumoniae strain in an immunocompetent german male patient without travel history to asia. phenotypic and molecular characterization showed high similarity to the reference genome ntuh-k2044 isolated in asia. the isolate was assigned as st2398 (clonal complex 66). the findings underline global spread of hypervirulent klebsiella pneumoniae strains to europe.201728392922
help is on the way: monoclonal antibody therapy for multi-drug resistant bacteria. 201728306387
the hospital water environment as a reservoir for carbapenem-resistant organisms causing hospital-acquired infections-a systematic review of the literature.over the last 20 years there have been 32 reports of carbapenem-resistant organisms in the hospital water environment, with half of these occurring since 2010. the majority of these reports have described associated clinical outbreaks in the intensive care setting, affecting the critically ill and the immunocompromised. drains, sinks, and faucets were most frequently colonized, and pseudomonas aeruginosa the predominant organism. imipenemase (imp), klebsiella pneumoniae carbapenemase (kpc), and ...201728200000
epithelial myeloid-differentiation factor 88 is dispensable during klebsiella pneumonia.klebsiella pneumoniae is a common cause of pneumonia. previous studies have documented an important role for toll-like receptors (tlrs) expressed by myeloid cells in the recognition of k. pneumoniae and the initiation of a protective immune response. lung epithelial cells also express tlrs and can participate in innate immune defense. the aim of this study was to examine the role of the common tlr adaptor protein myeloid-differentiation factor (myd) 88 in lung epithelium during host defense agai ...201728187270
mincle-mediated neutrophil extracellular trap formation by regulation of autophagy.neutrophil extracellular traps (nets) constitute antimicrobial function of neutrophils but have also been linked to perpetuation of inflammation. despite this evident physiological relevance, mechanistic understanding of net formation is poor. in this study, we examined the mechanism by which mincle, a c-type lectin receptor, regulates net formation.201728186242
implant-associated esbl-klebsiella pneumonia producing small colony variant bone and joint infection in a healthy 40-year-old man. 201728167688
determining β-lactam exposure threshold to suppress resistance development in gram-negative bacteria.β-lactams are commonly used for nosocomial infections and resistance to these agents among gram-negative bacteria is increasing rapidly. optimized dosing is expected to reduce the likelihood of resistance development during antimicrobial therapy, but the target for clinical dose adjustment is not well established. we examined the likelihood that various dosing exposures would suppress resistance development in an in vitro hollow-fibre infection model.201728158470
synthesis and in vitro activity of dicationic indolyl diphenyl ethers as novel potent antibiotic agents against drug-resistant bacteria.a series of 4,4'-bis-[2-(6-n-substituted-amidino)indolyl] diphenyl ether have been synthesized and tested for their in vitro antibacterial activity including a range of gram-positive and gram-negative pathogens and cytotoxicity. most of these compounds have mainly shown anti-gram positive bacteria activities especially against drug resistant bacterial strains mrsa, mrse and vre. the anti-mrsa and anti-mrse activities of compound 7a and 7j were more potent than that of the lead compound 2, levofl ...201728109787
quantitative optical coherence microscopy for the in situ investigation of the biofilm.this paper explores the potential of optical coherence microscopy (ocm) for the <italic<in situ</italic< monitoring of biofilm growth. the quantitative imaging of the early developmental biology of a representative biofilm, <italic<klebsiella pneumonia</italic< (kp-1), was performed using a swept source-based fourier domain ocm system. the growth dynamics of the kp-1 biofilms and their transient response under perturbation was investigated using the enface visualization of microcolonies and thei ...201627936266
detection of ctx-m gene antibiotics resistance in klebsiella pneumonia isolates of hospitals in adjara (georgia).research describing the epidemiology of antibiotic resistant microbes is vital to the proactive development of new antimicrobial agents. in the last years, ctx-m extended-spectrum β-lactamases (esbls) have emerged worldwide and have replaced classical tem and shv-type esbls in many countries. ctx-m-15 is currently the most frequent, with a pandemic distribution, and its rapid spread is facilitated by incorporation of resistance genes in mobile genetic elements. the esbl is efficacious in gram-ne ...201627770529
investigation of bacterial inactivation in apheresis platelets with 24 or 30 hours between inoculation and inactivation.blood centre logistics, staffing and donor scheduling may be optimized if pathogen inactivation (pi) of platelets can be delayed until day 1, but bacteria may rapidly grow during this time. this study evaluates bacterial pi performed 24 and 30 h after collection.201627185478
protective effect of inflammasome activation by hydrogen peroxide in a mouse model of septic study the effect of a lack of antioxidant defenses during lethal pneumonia induced by klebsiella pneumonia, compared to wild-type mice.201727749344
community acquired klebsiella pneumonia related psoas abscess. 201627728379
genotyping of klebsiella pneumonia strains isolated from eldly inpatients by multiple-locus variable-number tandem-repeat analysis.objective to investigate the genotype of klebsiella pneumonia strains isolated from eldly inpatients by multiple-locus variable-number tandem-repeat analysis. methods totally 184 klebsiella pneumonia strains,isolated from eldly inpatients,were collected,and their genome dna were extracted. the polymorphism of 7 variable-number tandem-repeat locus in the dna samples was analyzed by multiple primers polymerase chain reaction and capillary electrophoresis. the clustering analysis of genotyping was ...201627594157
minocycline and tigecycline: what is their role in the treatment of carbapenem-resistant gram-negative organisms?carbapenem-resistant organisms are increasingly common worldwide, particularly in india and are associated with high mortality rates especially in patients with severe infection such as bacteremia. existing drugs such as carbapenems and polymyxins have a number of disadvantages, but remain the mainstay of treatment. the tetracycline class of antibiotics was first produced in the 1940s. minocycline, tetracycline derivative, although licensed for treatment of wide range of infections, has not been ...201727564414
study of reactivity of cyanoacetohydrazonoethyl-n-ethyl-n-methyl benzenesulfonamide: preparation of novel anticancer and antimicrobial active heterocyclic benzenesulfonamide derivatives and their molecular docking against dihydrofolate reductase.this article describes the synthesis of some novel heterocyclic sulfonamides having biologically active thiophene 3, 4, 5, 6, coumarin 8, benzocoumarin 9, thiazole 7, piperidine 10, pyrrolidine 11, pyrazole 14 and pyridine 12, 13. starting with 4-(1-(2-(2-cyanoacetyl)hydrazono)ethyl)-n-ethyl-n-methylbenzenesulfonamide (2), which was prepared from condensation of acetophenone derivative 1 with 2-cyanoacetohydrazide. the structures of the newly synthesized compounds were confirmed by elemental ana ...201627557134
phytochemical composition, gc-ms analysis, in vitro antioxidant and antibacterial potential of clove flower bud (eugenia caryophyllus) methanolic extract.plant derived pharmacologically active compounds have gained importance in food and pharmaceutical industries. the aim of the present study is to identify and study the antioxidant, antimicrobial properties of the phytochemicals present in the crude extract of eugenia caryophyllus flower buds. the antioxidant activity of the methanol, acetone and chloroform extract was evaluated by 1,1-diphenyl-2-picrylhydrazyl (dpph) assay. the methanol extract showed better radical scavenging activity than oth ...201627162398
neonatal septic arthritis in a tertiary care hospital: a descriptive study.the study was conducted to evaluate clinical and microbiological profile of neonates with septic arthritis and also to assess changing epidemiology in the microbial etiology.201627154290
invasive klebsiella pneumoniae liver abscess syndrome. 201728757731
xpert carba-r assay for the detection of carbapenemase-producing organisms in intensive care unit patients of a korean tertiary care hospital.carbapenemase-producing organisms (cpo) are rapidly disseminating worldwide, and their presence in tertiary care hospitals poses a significant threat to the management of nosocomial infections. there is a need to control cpo, especially in intensive care unit (icu) patients, because these organisms are resistant to most β-lactam antibiotics and are easily transmitted. at present, the identification of cpo is time-consuming; hence, this study focused on the use of the xpert carba-r assay (cepheid ...201626709264
chronic klebsiella pneumonia: a rare manifestation of klebsiella pneumonia.k. pneumoniae can present as two forms of community-acquired pneumonia, acute and chronic. although acute pneumonia may turn into necrotizing pneumonia, which results in a prolonged clinical course, it often has a rapidly progressive clinical course. in contrast, chronic klebsiella pneumonia runs a protracted indolent course that mimics other chronic pulmonary infections and malignancies. herein, we present two cases of chronic klebsiella pneumonia. the diagnosis was made by microorganism identi ...201526543615
m2b monocytes provoke bacterial pneumonia and gut bacteria-associated sepsis in alcoholics.chronic alcohol consumption markedly impairs host antibacterial defense against opportunistic infections. γ-irradiated nod-scid il-2rγ(null) mice inoculated with nonalcoholic pbmcs (control pbmc chimeras) resisted klebsiella pneumonia and gut bacteria-associated sepsis, whereas the chimeras created with alcoholic pbmcs (alcoholic pbmc chimeras) were very susceptible to these infections. m1 monocytes (il-12(+)il-10(-)cd163(-)cd14(+) cells), major effector cells in antibacterial innate immunity, w ...201526525287
development of a cutaneous fistula following hepatic cystic echinococcosis.hydatid cyst is an infectious disease characterized by cysts formed primarly within the gastrointestinal tract by echinococci. hepatic hydatid disease, which is the most common form, remains asymptomatic until complications occur. in this report, we present an 80 years old patient who presented with a hepatic hydatid cyst which fistulized to the abdominal skin into the emergency department. computed tomography of the abdomen showed inactive grade 5 cyst. drainage without removal of the cyst fail ...201526413444
comparative whole genome transcriptome and metabolome analyses of five klebsiella pneumonia strains.the integration of transcriptomics and metabolomics can provide precise information on gene-to-metabolite networks for identifying the function of novel genes. the goal of this study was to identify novel gene functions involved in 2,3-butanediol (2,3-bdo) biosynthesis by a comprehensive analysis of the transcriptome and metabolome of five mutated klebsiella pneumonia strains (∆wabg = sgsb100, ∆wabg∆buda = sgsb106, ∆wabg∆budb = sgsb107, ∆wabg∆budc = sgsb108, ∆wabg∆budabc = sgsb109). first, the t ...201526296382
optimization of tannase production by a novel klebsiella pneumoniae kp715242 using central composite design.a novel tannase producing bacterial strain was isolated from rhizospheric soil of acacia species and identified as klebsiella pneumoniae kp715242. a 3.25-fold increase in tannase production was achieved upon optimization with central composite design using response surface methodology. four variables namely ph, temperature, incubation period, and agitation speed were used to optimize significant correlation between the effects of these variables on tannase production. a second-order polynomial w ...201528626722
a non-pathogenic and optically high concentrated (r,r)-2,3-butanediol biosynthesizing klebsiella strain.the objective of this work was to construct a non-pathogenic klebsiella pneumonia strain that can produce optically high concentrated (r,r)-2,3-bdo. a k. pneumonia mutant lacking the pathogenic factor was used as the host strain. in order to construct a k. pneumonia strain that would biosynthesize high concentrated (r,r)-2,3-bdo, gene deletion and over-expression methods were combined; firstly, the 2,3-bdo dehydrogenase (budc) gene was deleted to re-direct utilization of the carbon source to (r, ...201526074000
an outbreak of carbapenem-resistant oxa-48 - producing klebsiella pneumonia associated to duodenoscopy.carbapenemase-producing enterobacteriaceae (cpe) have become a major problem for healthcare systems worldwide. while the first reports from european hospitals described the introduction of cpe from endemic countries, there is now a growing number of reports describing outbreaks of cpe in european hospitals. here we report an outbreak of carbapenem-resistant k. pneumoniae in a german university hospital which was in part associated to duodenoscopy.201525815166
molecular epidemiology of aminoglycosides resistance on klebsiella pneumonia in a hospital in investigate the molecular epidemiology of aminoglycosides resistance among klebsiella pneumonia in hospitals in china, the antibiotics resistance and the possession of extended-spectrum β-lactamases (esbls) from 162 isolates were examined using kirby-bauer disk diffusion and pcr sequencing. overall, 47.5% (77/162) of strains showed an esbl phenotype. according to antibiotics resistance, esbls-positive k. pneumoniae showed significantly higher resistance to most antibiotics than esbls-negative ...201525785141
[isolation of a carbapenem-resistant k1 serotype klebsiella pneumonia strain and the study of resistance mechanism].to study the virulence and mechanism of carbapenem resistance of a clinical isolate of carbapenem-resistant k1 serotype klebsiella pneumonia strain.201425622962
modification of chimeric (2s, 3s)-butanediol dehydrogenase based on structural information.a chimeric (2s, 3s)-butanediol dehydrogenase (clbdh) was engineered to have the strict (s)-configuration specificity of the (2s, 3s)-bdh (bslbdh) derived from brevibacterium saccharolyticum as well as the enzymatic stability of the (2r, 3s)-bdh (kpmbdh) from klebsiella pneumonia by swapping the domains of two native bdhs. however, while clbdh possesses the stability, it lacks the specificity. in order to assist in the design a bdh having strict substrate specificity, an x-ray structural analysis ...201425612804
m2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.alcohol abuse was found to predispose persons to opportunistic infections. in this study, we tried to improve the host antibacterial resistance of chronic alcohol-consuming (cac) mice to opportunistic infections. bactericidal macrophages with functions to produce il-12 and to express mrnas for cxcl9 and inducible nitric oxide synthase (m1 macrophages) were characterized as the main effector cells in host antibacterial innate immunities against infections with opportunistic pathogens. however, ca ...201525485859
klebsiella pneumonia-induced prostate abscess: how to work it up?klebsiella pneumonia (kp) is related to a metastatic phenomenon from the originally affected primary organ. about 28% of patients with pyogenic liver abscess arising from kp suffer from metastatic complications. this study was done to define the clinical features of kp-induced prostate abscess.201425485013
raised incidence of ankylosing spondylitis among inuit populations could be due to high hla-b27 association and starch consumption.ankylosing spondylitis (as) is a chronic inflammatory arthritis mainly affecting the spinal joints. it would appear that the most likely causative agent in the development of as is an environmental factor in the genetically susceptible, hla-b27 positive, individuals. extensive data from several countries support the notion that klebsiella pneumonia bacteria are the most likely culprit in the causation of as. these microbes possess antigens which resemble hla-b27 and spinal collagens. increased i ...201525385438
immunoproteomic to analysis the pathogenicity factors in leukopenia caused by klebsiella pneumonia bacteremia.incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. immunoproteomic is a promising method to identify pathogenicity factors of different diseases. in the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by klebsiella pneumonia bacteremia. approximately 40 protein spots localized in the 4 to 7 pi range were detected on two-dimensional electrophoresis gels, ...201425330314
concurrent endophthalmitis and orbital cellulitis from metastatic klebsiella pneumonia liver abscess.a 70-year-old korean female with a history of klebsiella pneumonia liver abscess was presented to the authors' service with signs of endophthalmitis and orbital cellulitis. vitreous biopsy confirmed k. pneumonia as the causative organism. with prolonged intravenous antibiotics and steroids, orbital symptoms resolved, but visual acuity remained at light perception. this is the first case in the literature to report on endophthalmitis and orbital cellulitis from k. pneumonia liver abscess.201725186218
antimicrobial and cytotoxic evaluation of some herbal essential oils in comparison with common antibiotics in bioassay condition.since ancient times, various infectious diseases have been treated using herbal drugs. today, efforts regarding the discovery of the effectual components of plants possessing antimicrobial properties are advanced. herbal essential oils are widely used for treatment of various diseases, and they play an important role in health care considerations.201428664090
preliminary phytochemical screening, antimicrobial and antioxidant activities of euphorbia milli.euphorbia milii is a pakistani herb used for various infectious diseases. in this study we have carried out phytochemical, antibacterial and antioxidant investigation of different extracts/fractions. phytochemical studies showed the presence of cardiac glycosides, steroids/phytosterols, anthocyanin, proteins, terpenoids, flavonoids and tannins. susceptibility testing by well diffusion assay of its chloroform and methanol fractions revealed good antimicrobial activity against klebsiella pneumonia ...201425015465
[sometimes a liver can cause you headaches].we report on a 41-year-old patient who was taken over from an outside hospital with headache, fever, polydipsy and profound sweating.201424964252
string test of hypervirulent klebsiella pneumonia. 201424890554
polyvinylpyrrolidone oral films of enrofloxacin: film characterization and drug release.enrofloxacin is a fluoroquinolone derivative used for treating urinary tract, respiratory and skin infections in animals. however, low solubility and low bioavailability prevented it from using on humans. polyvinylpyrrolidone (pvp) is an inert, non toxic polymer with excellent hydrophilic properties, besides it can enhance bioavailability by forming drug polymer conjugates. with the aim of increasing solubility and bioavailability, enrofloxacin thin films were prepared using pvp as a polymer mat ...201424858388
retroperitoneoscopic drainage of bilateral psoas abscesses under intraoperative laparoscopic ultrasound guidance.despite improved diagnostic modalities for psoas abscesses, the optimum management strategy is not uniform. a 67-year-old man presented with bilateral psoas abscesses secondary to l1-l2 pyogenic discitis. on contrast-enhanced ct, the largest of these abscesses measured 13 × 14 × 33 mm on the right. the patient developed sepsis caused by klebsiella pneumonia. there were no signs of improvement after 3 weeks of systematic antibiotic administration. we performed surgical drainage of bilateral psoas ...201424754884
[clinical analysis of nosocomial infection and risk factors of extremely premature infants].to investigate the incidence of nosocomial infections of extremely premature infants and to explore the risk factors and strategies for infection control.201424739727
efficacy and safety of low-dose colistin in the treatment for infections caused by multidrug-resistant gram-negative bacteria.infections due to multidrug-resistant gram-negative bacteria (mdr-gnb) are a significant burden to the healthcare system globally. colistin is the drug of choice for mdr-gnb and recent studies recommend high doses. this study investigated the safety of low-dose colistin and the relationship of minimum inhibitory concentration (mic) of colistin with bacterial cure in the treatment for mdr-gnb infections.201424593154
pharmacokinetics and ex-vivo pharmacodynamics of cefquinome against klebsiella pneumonia in healthy dogs.a two-period cross-over study was carried to investigate the pharmacokinetics (pk) and ex-vivo pharmacodynamics (pd) of cefquinome when administrated intravenously (iv) and intramuscularly (im) in seven healthy dogs at a dose of 2 mg/kg of body weight. serum concentrations were determined by hplc-ms/ms assay and cefquinome concentration vs. time data after iv and im were best fit to a two-compartment open model. cefquinome mean values of area under concentration-time curve (auc) were 5.15 μg · h ...201424372291
heterogeneity of lung mononuclear phagocytes during pneumonia: contribution of chemokine receptors.bacterial pneumonia is a common and dangerous illness. mononuclear phagocytes, which comprise monocyte, resident and recruited macrophage, and dendritic cell subsets, are critical to antimicrobial defenses, but the dynamics of their recruitment to the lungs in pneumonia is not established. we hypothesized that chemokine-mediated traffic of mononuclear phagocytes is important in defense against bacterial pneumonia. in a mouse model of klebsiella pneumonia, circulating ly6c(hi) and, to a lesser ex ...201324056971
il-17a and th17 cells in lung inflammation: an update on the role of th17 cell differentiation and il-17r signaling in host defense against infection.the significance of th17 cells and interleukin- (il-)17a signaling in host defense and disease development has been demonstrated in various infection and autoimmune models. numerous studies have indicated that th17 cells and its signature cytokine il-17a are critical to the airway's immune response against various bacteria and fungal infection. cytokines such as il-23, which are involved in th17 differentiation, play a critical role in controlling klebsiella pneumonia (k. pneumonia) infection. i ...201323956759
synthesis and spectroscopic characterization of 3,4-difluoroacetophenone-thiosemicarbazone and its palladium(ii) complex: evaluation of antimicrobial and antitumour activity.a new cis-palladium(ii)diaqua(3,4-difluoroacetophenonethiosemicarbazone complex (pd(ii) complex) is synthesized using 3,4-difluoroacetophenonethiosemicarbazone(l). the l and its pd(ii) complex are characterized and confirmed by elemental analyses, electrochemical analyses, ft-ir, ft-raman, uv-vis, hrms and lc-ms techniques. ligand l is further characterized by (1)h, (13)c and (19)f nmr spectroscopy. the crystal structure of l is unambiguously characterized by single x-ray crystallography. the li ...201323871984
macular hole formation secondary to bacterial septic embolism demonstrated by serial spectral-domain optical coherence tomography describe a case of macular hole (mh) formation secondary to bacterial septic embolism and demonstrate the changing thereof using serial spectral-domain optical coherence tomography (sd-oct) images.201323697861
a liver abscess deprived a healthy adult of eyesight: endogenous endophthalmitis associated with a pyogenic liver abscess caused by serotype k1 klebsiella pneumonia.klebsiella pneumonia usually causes urinary tract infections, pneumonia, and other infectious diseases in hospitalized and immunocompromised patients. among the types of klebsiella pneumonia, serotype k1 is known to be a highly virulent pathogen. we herein report the case of a healthy 63-year-old man with a pyogenic liver abscess and bilateral endogenous endophthalmitis caused by serotype k1 klebsiella pneumonia. although the patient received percutaneous abscess drainage and antibiotic therapy, ...201323583997
perirenal haematoma with klebsiella pneumonia pyelonephritis. 201323291815
microwave synthesis, characterization and bio-efficacy evaluation of novel chalcone based 6-carbethoxy-2-cyclohexen-1-one and 2h-indazol-3-ol derivatives.novel chalcone based 6-carbethoxy-2-cyclohexen-1-one and 2h-indazol-3-ol derivatives were synthesized and characterized by using spectral techniques like ir, (1)h nmr, (13)c nmr, cosy, dept, and gc-ms. all these compounds were screened for anti-fungal, anti-bacterial and anti-oxidant activity. cyclohexenone derivatives, in general, showed better anti-fungal and anti-bacterial activity than parent chalcones. whereas, all the indazole derivatives showed very good anti-oxidant activity and some wer ...201323229055
myeloid-related protein-14 contributes to protective immunity in gram-negative pneumonia derived sepsis.klebsiella (k.) pneumoniae is a common cause of pneumonia-derived sepsis. myeloid related protein 8 (mrp8, s100a8) and mrp14 (s100a9) are the most abundant cytoplasmic proteins in neutrophils. they can form mrp8/14 heterodimers that are released upon cell stress stimuli. mrp8/14 reportedly exerts antimicrobial activity, but in acute fulminant sepsis models mrp8/14 has been found to contribute to organ damage and death. we here determined the role of mrp8/14 in k. pneumoniae sepsis originating fr ...201223133376
[protection and mechanism of fagopyrum cymosum on lung injury in rats with klebsiella pneumonia].to study the mechanism of protective effect of fagopyrum cymosum on lung injury induced by klebsiella pneumonia in rats.201223019909
skin tlr7 triggering promotes accumulation of respiratory dendritic cells and natural killer cells.the tlr7 agonist imiquimod has been used successfully as adjuvant for skin treatment of virus-associated warts and basal cell carcinoma. the effects of skin tlr7 triggering on respiratory leukocyte populations are unknown. in a placebo-controlled experimental animal study we have used multicolour flow cytometry to systematically analyze the modulation of respiratory leukocyte subsets after skin administration of imiquimod. compared to placebo, skin administration of imiquimod significantly incre ...201222927956
a potential substrate binding conformation of β-lactams and insight into the broad spectrum of ndm-1 delhi metallo-β-lactamase 1 (ndm-1) is a key enzyme that the pathogen klebsiella pneumonia uses to hydrolyze almost all β-lactam antibiotics. it is currently unclear why ndm-1 has a broad spectrum of activity. docking of the representatives of the β-lactam families into the active site of ndm-1 is reported here. all the β-lactams naturally fit the ndm-1 pocket, implying that ndm-1 can accommodate the substrates without dramatic conformation changes. the docking reveals two major binding mode ...201222825119
a one-year follow-up study on the incidence and risk of endophthalmitis after pyogenic liver abscess.although endophthalmitis secondary to pyogenic liver abscess (pla) is becoming a globally emerging infectious disease, population-based investigations evaluating the relationship between pla and endogenous endophthalmitis remain scarce. this study aimed to investigate the incidence and risk of endogenous endophthalmitis in patients with pla compared with unaffected individuals by using a nationwide, population-based dataset.201222817832
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