PMID(sorted ascending)
resolution of carbapenemase-producing klebsiella pneumoniae outbreak in a tertiary cancer center; the role of active surveillance. 201728693656
prevalence of carbapenem-resistant klebsiella pneumoniae (crkp) and the distribution of class 1 integron in their strains isolated from a hospital in central china.objective the aim of our study is to investigate the prevalence of carbapenem-resistant klebsiella pneumoniae (crkp) and the genetic characteristics of the class 1 integron in crkp on multi-drug resistance.methods clinical klebsiella pneumoniae strains were collected from multiple departments of a hospital in central china. crkp strains were identified among the isolates, and antibiotics susceptibility of crkp strains was analyzed. the polymerase chain reaction (pcr) was adopted to amplify the c ...201728693691
the prevalence of rectal carriage of klebsiella pneumoniae amongst diabetic patients and their clinical relevance in taiwan: a five-year prospective study.pyogenic liver abscess (pla) and bacteremia caused by klebsiella pneumoniae is a common complication among patients with diabetes mellitus (dm). the aim of this study is to investigate the prevalence of rectal carriage and serotype distribution of k. pneumoniae amongst dm patients and their clinical relevance.201728693930
antimicrobial natural product research: a review from a south african perspective for the years 2009-2016.this review provides information on the antimicrobial research which has taken place on south african natural products for the last eight years (2009-2016). this important field is the backbone of all studies involving the use of medicinal plants against infectious diseases and hence can form the mainstay for future studies.201728694104
double carbapenem therapy (dct) for bacteremia due to carbapenem-resistant klebsiella pneumoniae (crkp): from test tube to clinical practice. 201728695756
characterization of the complete nucleotide sequences of inca/c2 plasmids carrying in809-like integrons from enterobacteriaceae isolates of wildlife origin.a total of 18 enterobacteriaceae (17 from gulls and 1 from a clinical sample) collected from australia, carrying inca/c plasmids with the imp-encoding in809-like integrons, were studied. seven plasmids, being representatives of different origins, plasmid sizes, replicon combinations, and resistance genes, were completely sequenced. plasmid pec158, identified in a clinical escherichia coli st752 isolate, showed extensive similarity to type 2 inca/c2 plasmids. pec158 carried none of the blacmy-2-l ...201728696228
prevalence and etiology of bacteremia in febrile children with sickle cell disease at a nigeria tertiary a result of immune defects in sickle cell disease (scd), affected individuals are prone to infection from encapsulated bacterial pathogens like streptococcus pneumoniae. studies on the etiological agents of bacteremia in children with scd in nigeria are few and have revealed a spectrum of organisms that is different from those recorded in other parts of the world.201728698782
antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from ocimum basilicum var. thyrsiflora leaves.fourteen fungal endophytes were isolated from the ocimum basilicum var. thyrsiflora leaves collected from northern thailand. eight genera were identified including aspergillus, ascochyta, nigrospora, blastomyces, colletotrichum, exidia, clitopilus, and nomuraea. the antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus cereus, escherichia coli, klebsiella ...201728698913
antituberculotic activity of actinobacteria isolated from the rare habitats.a distinctive screening procedure resulted in the isolation and identification of antituberculotic actinobacteria. in this course, a total of 125 actinobacteria were isolated from various soil samples from untapped areas in northwestern himalayas, india. the antibacterial screening showed that 26 isolates inhibited the growth of at least one of the tested bacterial pathogens including staphylococcus aureus (atcc 25923), staphylococcus epidermidis (atcc 12228), bacillus subtilis (atcc 11774), mic ...201728699251
st37 klebsiella pneumoniae: development of carbapenem resistance in vivo during antimicrobial therapy in investigate the mechanism leading to in vivo carbapenem resistance development in klebsiella pneumoniae.201728699768
assessing antibiotic therapy effectiveness against the major bacterial pathogens in a hospital using an integrated assess the effectiveness of antibiotic therapy against five indicator bacteria in a chinese hospital using an index-based approach.201728699775
molecular detection of β-lactamase and integron genes in clinical strains of klebsiella pneumoniae by multiplex polymerase chain reaction.infections caused by β-lactamase-producing gram-negative bacteria, such as klebsiella pneumoniae, are increasing globally with high morbidity and mortality. the aim of the current study was to determine antimicrobial susceptibility patterns and the prevalence of antibiotic resistance genes (β-lactamase and integron genes) using multiplex pcr.201728700049
accelerated detection of extended-spectrum β-lactamases in clinical isolates of enterobacteriaceae.a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum β-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esblproducers by the standard clinical laboratory standards ...200628700883
bacterial bloodstream infections in a tertiary infectious diseases hospital in northern vietnam: aetiology, drug resistance, and treatment outcome.bloodstream infections (bsis) are associated with high morbidity and mortality worldwide. however their aetiology, antimicrobial susceptibilities and associated outcomes differ between developed and developing countries. systematic data from vietnam are scarce. here we present aetiologic data on bsi in adults admitted to a large tertiary referral hospital for infectious diseases in hanoi, vietnam.201728701159
antimicrobial susceptibility among gram-positive and gram-negative organisms collected from the latin american region between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial.the in vitro activity of tigecycline and comparator agents was evaluated against gram-positive and gram-negative isolates collected in latin american centers between 2004 and 2015 as part of the tigecycline evaluation and surveillance trial (t.e.s.t.) global surveillance study.201728701170
silver nanoparticles functionalized with ampicillin.the resistance of pathogenic bacteria to antibiotics has become a serious problem. the emphasis is placed on the development of new, effective antimicrobial strategies. one of them is the use of agnps in association with antibiotic drugs. the aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high-performance liquid chromatography approach. to confirm the occurrence of silver nanopartic ...201728704596
modified double-disc test for detection of extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae.this study evaluates the performance of a modified double-disc test (mddt) for the detection of extended-spectrum β-lactamases (esbls) in clinical isolates of escherichia coli and klebsiella pneumoniae. ninety-six isolates of e. coli and 40 k. pneumoniae are studied for esbl production by the national committee for clinical laboratory standards (nccls) combination disc tests and mddt. a total of 112 (82%) isolates (80 [83%] e. coli, 32 [80%] k. pneumoniae) were positive for esbl by mddt compared ...200628705137
development of silver nanoparticles loaded chitosan-alginate constructs with biomedical potentialities.herein, a facile biosynthesis of silver nanoparticles (agnps) and agnps-loaded chitosan-alginate constructs with biomedical potentialities is reported. the uv-vis spectroscopic profile confirmed the synthesis of agnps using methanolic leaves extract of euphorbia helioscopia. the newly developed agnps were characterized using various analytical and imaging techniques including uv-vis and ft-ir spectroscopy, x-ray diffraction (xrd), scanning electron microscopy (sem), energy dispersive spectroscop ...201728705499
prevalence and risk factors for colonization by clostridium difficile and extended-spectrum β-lactamase-producing enterobacteriaceae in rehabilitation clinics in germany.rehabilitation clinics may vary widely in terms of type of care provided, duration of hospital stay, and case severity. few data are available on prevalence of clostridium difficile or extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-e) colonization in rehabilitation clinics in germany.201728705583
in-vitro activity of ceftolozane/tazobactam against pseudomonas aeruginosa and enterobacteriaceae isolates recovered from hospitalized patients in evaluate the activity of ceftolozane/tazobactam in comparison with other broad-spectrum antimicrobials against pseudomonas aeruginosa and enterobacteriaceae, 497 non-duplicate p. aeruginosa and 802 enterobacteriaceae clinical isolates were consecutively collected during the period from september 2014 to april 2015 from patients in germany with bloodstream, lower respiratory tract, intraabdominal or urinary tract infections. antimicrobial susceptibility testing was performed by broth microdilu ...201728705666
activity of temocillin against ctx-m-producing escherichia coli and klebsiella pneumoniae from germany. 201728705669
the plasmid-mediated fosfomycin resistance determinants and synergy of fosfomycin and meropenem in carbapenem-resistant klebsiella pneumoniae isolates in taiwan.epidemiology of fosfomycin susceptibility and the plasmid-mediated fosfomycinase genes of carbapenem-resistant klebsiella pneumoniae (crkp) isolates in taiwan remain unclear.201728705769
draft whole-genome assemblies of drug-resistant clinical isolates of klebsiella pneumoniae from the, we report the draft assemblies of 11 clinical isolates of klebsiella pneumoniae that are resistant to cephalosporins, carbapenems, and/or colistin. the assemblies ranged from 5.37 mbp to 5.70 mbp in size. several plasmid sequences were present, and resistance genes spanning multiple classes of antibiotics were predicted.201728705960
detection of extended-spectrum beta-lactamases (esbls) and antibiotic susceptibility patterns in klebsiella pneumoniae in western, iran.production of beta-lactamase enzymes, especially extended-spectrum beta -lactamases (esbl), is a primary mechanism of resistance in these bacteria. the purpose of this study was detection of blatem, blashv, blactx-m, blactx-m-15, blaper and blaveb in k. pneumoniae, isolated from clinical specimens by the pcr method and antibiotic susceptibility patterns in these strains isolated, in sanandaj city, iran.201728707597
arsenic-resistant and plant growth promoting firmicutes and γ-proteobacteria species from industrially polluted irrigation water and corresponding cropland.the aim of the study was to explore irrigation water polluted with industrial waste and corresponding cropland to screen bacteria for as-detoxification and plant growth promotion.201728708308
effectiveness of a double-carbapenem regimen in a kpc-producing klebsiella pneumoniae infection in an immunocompromised patient.the progressive increase of infections produced by extensively drug-resistant carbapenemase-producing klebsiella pneumoniae (xdr-cpkp) represents an important threat to public health. unfortunately, optimal therapeutic options are scarce. retrospective studies have recommended combined therapy with more than one antibiotic and, more recently, a double-carbapenem regimen has been reported to be an effective alternative therapy. here, we describe an episode of sepsis in an immunocompromised patien ...201728708458
a long-term study of the diversity of oxa-48-like carbapenemase-producing bacterial strains in infected patients and carriers.since their emergence at the beginning of the century, oxa-48 carbapenemases have spread in the community and in hospitals. to assess the diversity of oxa-48-producing bacterial strains and plasmids in the hospital setting, we studied the strains isolated from patients in three hospitals in the paris area.201728708476
bad bacteria in acute appendicitis: rare but relevant.bacterial infections are a factor for morbidity in patients with acute appendicitis (aa). the spreading of multidrug-resistant (mdr) bacteria is a significant problem in surgery, and the most relevant mdr pathogens are summarized as enterobacteriaceae, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and enterococci (eskape) bacteria. data regarding the species and distribution of bacteria in aa are available, but information about the resistances an ...201728710611
outbreak of colistin-resistant carbapenemase-producing klebsiella pneumoniae in tunisia.mechanisms of colistin and carbapenem resistance among a collection of klebsiella pneumoniae isolates recovered in a university hospital in tunisia were studied.201728711559
introduction of sample tubes with sodium azide as a preservative for ethyl glucuronide in urine.ethyl glucuronide (etg) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. however, the instability of etg in urine against bacterial degradation or the post-collectional synthesis of etg in contaminated samples may cause false interpretation of etg results in urine samples. this study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism ta ...201728712037
a novel antibacterial peptide derived from crocodylus siamensis hemoglobin hydrolysate induces membrane permeabilisation causing iron dysregulation, oxidative stress and bacterial death.a novel antibacterial peptide from crocodylus siamensis hemoglobin hydrolysate (chhs) was characterised for antimicrobial activity.201728712131
phenotypic and genotypic characterization of clinically relevant bacteria isolated from dental waste and waste workers' hands, mucosas and coats.infectious wastes are potential sources of pathogenic micro-organisms, which may represent a risk to the professionals who manage them. in this study, we aimed to characterize the infectious bacteria present in dental waste and waste workers. the dental waste produced over 24 h was collected and waste workers were sampled by swabbing. isolate resistance profiles were characterized by vitek(®) and pcr and biofilm formation by congo red agar, string test and microtitre assay. to assess similarity ...201728712134
a hospital-based matched case-control study to identify risk factors for clinical infection with oxa-48-producing klebsiella pneumoniae in rectal carriers.asymptomatic colonisation of the gastrointestinal tract by carbapenemase-producing enterobacteriaceae is an important reservoir for transmission, which may precede infection. this retrospective observational case-control study was designed to identify risk factors for developing clinical infection with oxa-48-producing klebsiella pneumoniae in rectal carriers during hospitalisation. case patients (n = 76) had carbapenemase-producing k. pneumoniae (cpkp) infection and positive rectal culture for ...201728712369
extensively drug-resistant klebsiella pneumoniae causing nosocomial bloodstream infections in china: molecular investigation of antibiotic resistance determinants, informing therapy, and clinical outcomes.the rise in diversity of antimicrobial resistance phenotypes seen in klebsiella pneumoniae is becoming a serious antibiotic management problem. we sought to investigate the molecular characteristics and clinical implications of extensively drug-resistant (xdr) k. pneumoniae isolated from different nosocomial bloodstream infections (bsis) patients from july 2013 to november 2015. even in combination treatment, meropenem did not protect against mortality of bsis patients (p = 0.015). in contrast, ...201728713357
synthetic multispecies microbial communities reveals shifts in secondary metabolism and facilitates cryptic natural product discovery.chemically mediated interactions have been hypothesized to be essential for ecosystem functioning as co-occurring organisms can influence the performance of each other by metabolic means. here, we present a co-culture device that allows co-culturing of microorganisms that are physically separated but can exchange chemical signals and metabolites. this setup was adopted to perform investigations on the secondary metabolisms of both a fungal-bacterial community and an actinomycetic-actinomycetic c ...201728714207
clinical outcomes associated with carbapenem resistant klebsiella pneumoniae (crkp) in abdominal solid organ transplant (sot) recipients. 201728714754
production of chemicals by klebsiella pneumoniae using bamboo hydrolysate as feedstock.bamboo is an important biomass, and bamboo hydrolysate is used by klebsiella pneumoniae as a feedstock for chemical production. here, bamboo powder was pretreated with naoh and washed to a neutral ph. cellulase was added to the pretreated bamboo powder to generate the hydrolysate, which contained 30 g/l glucose and 15 g/l xylose and was used as the carbon source to prepare a medium for chemical production. when cultured in microaerobic conditions, 12.7 g/l 2,3-butanediol was produced by wildtype ...201728715380
comparison of synergism between colistin, fosfomycin and tigecycline against extended-spectrum β-lactamase-producing klebsiella pneumoniae isolates or with carbapenem investigate the synergistic and bactericidal effects of antimicrobial combinations of any two of colistin, fosfomycin and tigecycline against the nine extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae (kp) clinical isolates, including 4 carbapenem-susceptible strains and five imipenem and/or meropenem-resistant strains.201728716360
phosphoenolpyruvate phosphotransferase system components positively regulate klebsiella biofilm formation.klebsiella pneumoniae is one of the leading causes of device-related infections (dris), which are associated with attachment of bacteria to these devices to form a biofilm. the latter is composed of not only bacteria but also extracellular polymeric substances (epses) consisting of extracellular dnas, polysaccharides, and other macromolecules. the phosphoenolpyruvate (pep):carbohydrate phosphotransferase system (pts) regulates diverse processes of bacterial physiology. in the genome of k. pneumo ...201728716362
the metabolite transporter peg344 is required for the full virulence of the hypervirulent klebsiella pneumoniae strain hvkp1 after pulmonary, but not subcutaneous challenge.hypervirulent klebsiella pneumoniae (hvkp) is an emerging pathotype that is capable of causing tissue-invasive, organ and life threatening infections in healthy individuals from the community. knowledge on the virulence factors specific to hvkp is limited. in this report we describe a new factor (peg344) that increases the virulence of the hvkp strain hvkp1. peg344 is present on the hvkp1 virulence plasmid, is broadly prevalent among hvkp strains, and has increased rna abundance when grown in hu ...201728717029
new delhi metallo-beta-lactamase (ndm-1)-producing klebsiella pneumoniae isolated from a burned patient.background infections affecting burn patients are frequently caused by staphylococcus aureus, pseudomonas aeruginosa, and enterobacteriaceae species. infections with these pathogens have become increasingly difficult to treat due to evolving antibiotic resistance mechanisms, including the production of carbapenemases. case report the present case report describes the evolution of a burn patient with polymicrobial healthcare-associated burn infections, including a bloodstream infection due to an ...201728717120
isolation and in vitro evaluation of bacteriophages against mdr-bacterial isolates from septic wound infections.multi-drug resistance has become a major problem for the treatment of pathogenic bacterial infections. the use of bacteriophages is an attractive approach to overcome the problem of drug resistance in several pathogens that cause fatal diseases. our study aimed to isolate multi drug resistant bacteria from patients with septic wounds and then isolate and apply bacteriophages in vitro as alternative therapeutic agents. pus samples were aseptically collected from rajiv gandhi institute of medical ...201728719657
reaction-diffusion theory explains hypoxia and heterogeneous growth within microbial biofilms associated with chronic infections.reaction-diffusion models were applied to gain insight into the aspects of biofilm infection and persistence by comparing mathematical simulations with the experimental data from varied bacterial biofilms. these comparisons, including three in vitro systems and two clinical investigations of specimens examined ex vivo, underscored the central importance of concentration gradients of metabolic substrates and the resulting physiological heterogeneity of the microorganisms. relatively simple one-di ...201628721248
identification of antipneumococcal molecules effective against different streptococcus pneumoniae serotypes using a resazurin-based high-throughput screen.streptococcus pneumoniae is a major human pathogen, causing around 1.6 million deaths worldwide each year. by optimizing a resazurin-based assay to detect s. pneumoniae growth in 384-well microplates, we developed a new high-throughput screening (hts) system for the discovery of antipneumococcal molecules, which was unsuccessful using conventional absorbance measurements. before applying our protocol to a large-scale screen, we validated the system through a pilot screen targeting about 7,800 bi ...201728723269
long-term care facility (ltcf) residents colonized with multidrug-resistant (mdr) klebsiella pneumoniae lineages frequently causing infections in portuguese clinical institutions. 201728724454
antimicrobial efficacy and synergy of metal ions against enterococcus faecium, klebsiella pneumoniae and acinetobacter baumannii in planktonic and biofilm phenotypes.the effects of metal ion solutions (silver, copper, platinum, gold and palladium) were determined individually and in combination against enterococcus faecium, acinetobacter baumannii and klebsiella pneumoniae. platinum, gold and palladium showed the greatest antimicrobial efficacy in zone of inhibition (zoi) assays. when tested in combinations using zoi assays, gold/platinum, gold/palladium and platinum/palladium were indicative of synergy. microbial inhibitory concentration demonstrated platin ...201728724953
genomic characterization of novel incfii-type multidrug resistant plasmids p0716-kpc and p12181-kpc from klebsiella pneumoniae.this study aimed to genetically characterize two fully-sequenced novel incfii-type multidrug resistant (mdr) plasmids, p0716-kpc and p12181-kpc, recovered from two different clinical klebsiella pneumoniae isolates. p0716-kpc and p12181-kpc had a very similar genomic content. the backbones of p0716-kpc/p12181-kpc contained two different replicons (belonging to a novel incfii subtype and the rep_3 family), the incfiik and incfiiy maintenance regions, and conjugal transfer gene sets from incfiik-ty ...201728725038
genomic epidemiology of global klebsiella pneumoniae carbapenemase (kpc)-producing escherichia coli.the dissemination of carbapenem resistance in escherichia coli has major implications for the management of common infections. bla kpc, encoding a transmissible carbapenemase (kpc), has historically largely been associated with klebsiella pneumoniae, a predominant plasmid (pkpqil), and a specific transposable element (tn4401, ~10 kb). here we characterize the genetic features of bla kpc emergence in global e. coli, 2008-2013, using both long- and short-read whole-genome sequencing. amongst 43/45 ...201728725045
serum albumin and osmolality inhibit bdellovibrio bacteriovorus predation in human serum.we evaluated the bactericidal activity of bdellovibrio bacteriovorus, strain hd100, within blood sera against bacterial strains commonly associated with bacteremic infections, including e. coli, klebsiella pneumoniae and salmonella enterica. tests show that b. bacteriovorus hd100 is not susceptible to serum complement or its bactericidal activity. after a two hour exposure to human sera, the prey populations decreased 15- to 7,300-fold due to the serum complement activity while, in contrast, the ...201728725056
chemical constituents of helichrysum italicum (roth) g. don essential oil and their antimicrobial activity against gram-positive and gram-negative bacteria, filamentous fungi and candida albicans.the aerial parts of helichrysum italicum (roth) g. don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. the essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). the most o ...201728725151
mcr-1 and blakpc-3 in escherichia coli sequence type 744 after meropenem and colistin therapy, portugal.escherichia coli ec36 was recovered from a patient in portugal after treatment with meropenem and colistin. besides an incf plasmid with tn1441d-blakpc-3, already reported in clinical strains in this country, e. coli ec36 co-harbored an incx4::mcr-1 gene. results highlight emerging co-resistance to carbapenems and polymyxins after therapy with drugs from both classes.201728726622
spontaneous globe rupture due to rapidly evolving endogenous hypermucoid klebsiella pneumoniae this retrospective report, the authors describe a rare case of spontaneous globe rupture from klebsiella pneumoniae endophthalmitis in a middle-aged man with poorly controlled type 2 diabetes mellitus. there have been only four previously reported cases of spontaneous globe rupture from endophthalmitis. out of the now five reported cases, all have been due to endogenous endophthalmitis, four have been due to gram-negative bacteria, and three have been due to k. pneumoniae. k. pneumoniae, espe ...201728728189
[pyogenic liver abscess: changes of organisms and consequences for diagnosis and therapy].liver abscess is a rare but potentially fatal disease with mortality rates of 2 - 12 %. the spectrum of causative microorganisms varies across the world and is changing due to increasing worldwide travel activity. as a result, previously less frequent pathogens must increasingly be considered in the differential diagnosis. the most common pathogens of liver abscess are so-called abscess streptococci, anaerobes and gram-negative bacteria such as escherichia coli and klebsiella pneumoniae. certain ...201728728202
antimicrobial resistance patterns of gram-negative bacteria isolated from bloodstream infections in an iranian referral pediatric hospital: a six-year study.bloodstream infections (bsis) are considered as a major cause of morbidity and mortality in children all over the world. the aim of this study was to describe the epidemiology and antimicrobial resistance patterns of gram-negative bacteria (gnb) isolated from bloodstream infections (bsis) in children admitted to the children medical center hospital (cmc), tehran, iran.201728729206
first two cases of severe multifocal infections caused by klebsiella pneumoniae in switzerland: characterization of an atypical non-k1/k2-serotype strain causing liver abscess and endocarditis.we describe the first two multifocal invasive infections due to klebsiella pneumoniae recently observed in switzerland.201728729207
detection of multidrug resistant (mdr) bacteria in untreated waste water disposals of hospitals in dhaka city, bangladesh.emergence of mdr bacteria is a global problem and a major burden for treatment of various infectious diseases. this study was performed to detect antibiotic resistant bacteria in untreated hospital waste.201728729208
relating switching rates between normal and persister cells to substrate and antibiotic concentrations: a mathematical modelling approach supported by experiments.we developed and compared two mathematical models of variable phenotypic switching rates between normal and persister cells that depend on substrate concentration and antibiotic presence. they could be used to simulate the formation of persisters in environments with concentration gradients such as biofilms. our models are extensions of a previous model of the dynamics of normal and persistent cell populations developed by balaban et al. (2004, science 305: 1622). we calibrated the models' param ...201728730700
biofilm formation and antibiotic resistance in klebsiella pneumoniae urinary strains.multidrug-resistant klebsiella pneumoniae has become a relevant healthcare-associated pathogen. capsule, type 1 and 3 fimbriae (mrka gene), type 2 quorum-sensing system (luxs), synthesis of d-galactan i (wbbm), lps transport (wzm) and poly-beta-1,6-n-acetyl-d-glucosamine (pgaa) seem involved in k. pneumoniae biofilm. nonenzymatic antibiotic resistance is related to nonexpression or mutation of porins (ompk35 and ompk36), and efflux pump (acrb) overexpression. the aim of this study was to analyse ...201728731269
the kp1_4563 gene is regulated by the camp receptor protein and controls type 3 fimbrial function in klebsiella pneumoniae ntuh-k2044.klebsiella pneumoniae (k. pneumoniae) is an opportunistic pathogen that can adhere to host cells or extracellular matrix via type 1 and type 3 fimbriae. kp1_4563 is a gene encoding a hypothetical protein in k. pneumoniae ntuh-k2044. kp1_4563 is located between the type 1 and type 3 fimbrial gene clusters and is likely associated with fimbrial function given its putative conserved domains of unknown function (duf1471). cyclic amp receptor protein (crp) regulates virulence-related gene expression ...201728732013
occurrence and clonal diversity of multidrug-resistant klebsiella pneumoniae recovered from inanimate surfaces in algerian hospital environment: first report of arma, qnrb and aac(6')-ib-cr genes.the aim of this study is to characterize the molecular support of antibiotic resistance in mdr klebsiella pneumoniae recovered from inanimate surfaces between march 2012 to february 2014 in three teaching hospitals (setif, bejaia and constantine) in algeria.201728732788
seven new lignan glycosides from the branches of alangium kurzii craib var. new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ...201728733150
response to letter of k. singh, 'role of silver nitrate in the efficacy of hydrogen peroxide aerial decontamination systems' regarding s. ali et al. 'efficacy of two hydrogen peroxide vapour aerial decontamination systems for enhanced disinfection of meticillin-resistant staphylococcus aureus, klebsiella pneumoniae and clostridium difficile in single isolation rooms'. 201728734723
antimicrobial resistance profiles and genotypes of extended-spectrum β-lactamase- and ampc β-lactamase-producing klebsiella pneumoniae isolated from dogs in beijing, china.klebsiella pneumoniae, which exists in the intestinal and respiratory tracts of humans and animals, is an important conditional pathogen in many animals. the aim of the current study was to investigate the antimicrobial resistance profiles and genotypes of extended-spectrum β-lactamase (esbl)- and ampc β-lactamase-producing k. pneumoniae isolated from dogs.201728735050
early kpc-producing klebsiella pneumoniae bacteremia among intensive care unit patients non-colonized upon admission.among 140 patients colonized by kpc-producing klebsiella pneumoniae (kpc-kp) between fourth and seventh day of intensive care unit stay, 24 developed bacteraemia immediately after colonization. colistin-resistance of the colonizing isolate was the factor significantly associated with early kpc-kp bacteraemia (p < 0.001; or 6.6, 95% ci 2.4-18.4), a worrisome finding since infections by colistin-resistant isolates is associated with increased mortality due to limited remaining therapeutic options.201728735313
trends of bloodstream infections in a university greek hospital during a three-year period: incidence of multidrug-resistant bacteria and seasonality in gram-negative predominance.the aim of the study was to assess the epidemiology, the incidence of multidrug-resistant bacteria and bloodstream infections' (bsis) seasonality in a university hospital. this retrospective study was carried out in the university general hospital of patras, greece, during 2011-13 y. blood cultures from patients with clinical presentation suggestive of bloodstream infection were performed by the bact/alert system. isolates were identified by vitek 2 advanced expert system. antibiotic susceptibil ...201728735318
evaluation of anthelmintic antimicrobial and antioxidant activity of chenopodium album.the objective of this study was to evaluate the anthelmintic, antimicrobial and antioxidant activities of chenopodium album against gastrointestinal nematodes of sheep and some pathogenic microbes. a worm motility inhibition assay was used for in vitro study, and a faecal egg count reduction assay was used for an in vivo study. various concentrations ranging from 100 to 500 μg/ml of the extract were subjected to antimicrobial screening by disc diffusion method against four selected bacterial (st ...201728735356
hypermucoviscosity, rmpa, and aerobactin are associated with community-acquired klebsiella pneumoniae bacteremic isolates causing liver abscess in singapore.this retrospective study investigated the clinical etiology of community-acquired bacteremic klebsiellapneumoniae infections, and characterized laboratory and genetic markers which may be associated with primary liver abscess (pla).201728736222
prevalence of a new variant oxa-204 and oxa-48 carbapenemases plasmids encoded in klebsiella pneumoniae clinical isolates in tunisia.carbapenemase-producing klebsiella pneumoniae strains have emerged as a major problem for healthcare systems. the aim of this study was to determine the role and diversity of plasmids harboring carbapenemases encoding genes from a collection of k. pneumoniae isolates recovered between july 2011 and january 2012, with decreased susceptibility to carbapenems. imipenem (ipm), ertapenem (etp), meropenem (mem), and doripenem (dor) minimum inhibitory concentrations (mics) were determined by e-test. ca ...201728737463
colistin resistance in kpc-2- and shv-5-producing klebsiella pneumoniae clinical isolates in bulgaria.colistin resistance is increasingly recognized among carbapenemase-producing klebsiella pneumoniae isolates in several european regions. the current study documents the appearance of colistin resistance among kpc-2 and shv-5-produning k. pneumoniae strains in bulgaria.201728738348
antimicrobial, modulatory and chemical analysis of the oil of croton limae.croton sp. are plants with a well-reported antimicrobial activity. croton limae a.p. gomes, m.f. sales p.e. berry (euphorbiaceae), known as 'marmeleiro-prateado', is commonly used to manage abdominal pain in brazil.201728738715
esbl-producing escherichia coli and klebsiella pneumoniae: the most prevalent clinical isolates obtained between 2005 and 2012 in identify the prevalence of esbl genes in the principal group of enterobacteriaceae causing nosocomial infections and to identify the phylogenetic group in escherichia coli isolates.201728739224
resistance to ceftazidime-avibactam is due to transposition of kpc in a porin-deficient strain of klebsiella pneumoniae with increased efflux activity.ceftazidime-avibactam is an antibiotic with activity against serine beta-lactamases, including kpc. recently, reports have emerged of kpc-producing isolates resistant to this antibiotic, including a report of a wild-type kpc-3 producing sequence type 258 klebsiella pneumoniae that was resistant to ceftazidime-avibactam. we describe a detailed analysis of this isolate, in the context of two other closely related kpc-3 producing isolates, recovered from the same patient. both isolates encoded a no ...201728739787
retention of virulence following colistin adaptation in klebsiella pneumoniae is strain-dependent rather than associated with specific mutations.this study aimed to understand the impact on virulence and fitness of mutations in specific genes found after adaptation of klebsiella pneumoniae to colistin. isolates with an increase in their inhibitory concentration (mic) to colistin of 32- to >128-fold were shown to have mutations in mgrb, phopq and pmrab, all known regulators of pathways affecting membrane lipid content. when these strains were used in studies in galleria mellonella there was no clear correlation between mutations in specif ...201728741998
multidrug resistant bacteria are sensitive to euphorbia prostrata and six others cameroonian medicinal plants extracts.multidrug resistant (mdr) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them.201728743314
molecular characterization and functional analysis of the hepcidin gene from roughskin sculpin (trachidermus fasciatus).hepcidin is a kind of cysteine-rich antimicrobial peptide that plays a vital role in host innate immune activity and iron regulation. here, we report the molecular characterization and functional analysis of a novel hamp1 hepcidin isoforms tf-hep from roughskin sculpin, trachidermus fasciatus. a cdna fragment of 988 bp with an orf of 273 bp was obtained. the coding sequence encodes for a signal peptide of 24 amino acids coupled with a prodomain of 40 amino acids and a mature peptide of 26 amino ...201728743631
molecular epidemiology of carbapenem-resistant klebsiella pneumonia at a turkish center: is the increase of resistance a threat for europe?in recent years carbapenem resistant klebsiella pneumoniae has became an important threat to hospitalized patients. our aim in this study was to identify the genetic mechanisms of carbapenem resistance in our center.201728743652
occurrence of the mcr-1 colistin resistance gene and other clinically relevant antibiotic resistance genes in microbial populations at different municipal wastewater treatment plants in wastewater treatment plants (wwtps) with different population equivalents and catchment areas were screened for the prevalence of the colistin resistance gene mcr-1 mediating resistance against last resort antibiotic polymyxin e. the abundance of the plasmid-associated mcr-1 gene in total microbial populations during water treatment processes was quantitatively analyzed by qpcr analyses. the presence of the colistin resistance gene was documented for all of the influent wastewater samples ...201728744270
biomimetic synthesis of agnps from penicillium chrysogenum strain fgcc/bls1 by optimising physico-cultural conditions and assessment of their antimicrobial potential.biomimetic synthesis of metal nanoparticles (nps) is safe and eco-friendly; therefore, find diverse applications. considering this, the soil fungi penicillium chrysogenum strain fungal germplasm collection centre/ bls1 was isolated, characterized and explored to synthesize extracellular silver nps (agnps) under optimised conditions. the synthesis of agnps was investigated using ultraviolet (uv)-visible spectroscopy, fourier-transform infra-red spectroscopy (ftir), transmission electron microscop ...201728745292
recovery of metallo-tolerant and antibiotic resistant psychrophilic bacteria from siachen glacier, pakistan.cultureable bacterial diversity of previously unexplored siachen glacier, pakistan, was studied. out of 50 isolates 33 (66%) were gram negative and 17 (34%) gram positive. about half of the isolates were pigment producers and were able to grow at 4-37°c. 16s rrna gene sequences revealed gram negative bacteria dominated by proteobacteria (especially γ-proteobacteria and β-proteobacteria) and flavobacteria. the genus pseudomonas (51.51%, 17) was dominant among γ- proteobacteria. β-proteobacteria c ...201728746396
instability of the incfii-type plasmid carrying blandm-5 in a klebsiella pneumoniae this study, we characterized the blandm-5-bearing plasmid in a klebsiella pneumoniae isolate that had lost the plasmid during serial passage. we determined the complete sequences of the plasmid pcc1410-2, which was extracted from a k. pneumoniae st709 isolate collected at a korean hospital from which two ndm-5-producing k. pneumoniae isolates were subsequently isolated. as a result, the pcc1410-2 plasmid had a backbone structure that was similar to those of two plasmids previously reported fr ...201728746989
discovery of monoclonal antibodies cross-reactive to novel subserotypes of k. pneumoniae o3.klebsiella pneumoniae is responsible for nosocomial infections causing significant morbidity and mortality. treatment of newly emerging multi-drug resistant strains is hampered due to severely limited antibiotic choices. passive immunization targeting lps o-antigens has been proposed as an alternative therapeutic option, given the limited variability of klebsiella o-antigens. here we report that the o3 serogroup, previously considered to have uniform o-antigen built of mannan, represents three d ...201728747785
klebsiella pneumoniae isolates from meningitis: epidemiology, virulence and antibiotic resistance.klebsiella pneumoniae (kp) resistance to broad-spectrum cephalosporin (bsc) in meningitis is important because of limited therapeutic options. to investigate the antibiotic resistance, virulence and epidemiology of kp in meningitis, we conducted a retrospective study for 33 non-metastatic isolates, including primary meningitis (n = 20) and post-craniotomy meningitis (n = 13) collected from 1999 to 2013. bsc resistance was found in 9 (27.3%) isolates, all from post-craniotomy meningitis, harborin ...201728747788
antimicrobial effects of lysophosphatidylcholine on methicillin-resistant staphylococcus aureus.methicillin-resistant staphylococcus aureus (mrsa) is an important health care-associated and community-associated pathogen and causes a large number of infections worldwide. for the purpose of application to topical treatment of mrsa infection, we examined the antimicrobial effects of lysophosphatidylcholine (lpc) on mrsa strains. we also investigated the combination effect of lpc and gentamicin on mrsa growth.201728748087
activity of the novel siderophore cephalosporin cefiderocol against multidrug-resistant gram-negative pathogens.the novel siderophore cephalosporin cefiderocol (s-649266) with potent activity against gram-negative pathogens was recently developed (shionogi & co., ltd.). here, we evaluated the activity of this new molecule and comparators against a collection of previously characterized gram-negative isolates using broth microdilution panels. a total of 753 clinical multidrug-resistant gram-negative isolates collected from hospitals worldwide were tested against cefiderocol and antibiotic comparators (ceft ...201728748397
risk factors for carbapenem-resistant klebsiella pneumoniae infection: a meta-analysis.carbapenem-resistant klebsiella pneumoniae (crkp) infection has been rapidly emerging as a life-threatening nosocomial disease in many countries. however, studies on the corresponding risk factors of crkp infection showed inconsistent results. to resolve these inconsistencies, we conducted a meta-analysis of previous studies on the potential risk factors of crkp infection. the results of this study could be used to develop crkp infection prevention strategies.201728749714
competition is the basis of the transport mechanism of the nhab na+/h+ exchanger from klebsiella exchange is essential for survival of all organisms, having a role in the regulation of the intracellular na+ concentration, ph and cell volume. furthermore, na+/h+ exchangers were shown to be involved in the virulence of the bacterium yersinia pestis, indicating they might be potential targets for novel antibiotic treatments. the model system for na+/h+ exchangers is the nhaa transporter from escherichia coli, ecnhaa. therefore, the general transport mechanism of nhaa exchangers is curre ...201728750048
staphylococcus aureus single-stranded dna-binding protein ssba can bind but cannot stimulate pria helicase.single-stranded dna-binding protein (ssb) and pria helicase play important roles in bacterial dna replication restart process. the mechanism by which pria helicase is bound and stimulated by ssb in escherichia coli (ec) has been established, but information on this process in gram-positive bacteria are limited. we characterized the properties of ssb from staphylococcus aureus (sassba, a counterpart of ecssb) and analyzed its interaction with sapria. the gel filtration chromatography analysis of ...201728750050
polymyxins for cns infections: pharmacology and neurotoxicity.central nervous system (cns) infections caused by multi-drug resistant (mdr) gram-negative bacteria present a major health and economic burden worldwide. due to the nearly empty antibiotic discovery pipeline, polymyxins (i.e. polymyxin b and colistin) are used as the last-line therapy against gram-negative 'superbugs' when all other treatment modalities have failed. the treatment of cns infections due to multi-drug resistant gram-negative bacteria is problematic and associated with high mortalit ...201728750947
intra-abdominal infection after radical gastrectomy for gastric cancer: incidence, pathogens, risk factors and outcomes.surgical site infection, particularly intra-abdominal infection (iai), remains a clinically important event after gastrectomy for gastric cancer. the aim of this retrospective study was to clarify the incidence, pathogens, risk factors and outcomes of iai following gastrectomy for gastric cancer.201728751223
in-vivo loss of carbapenem resistance by extensively drug-resistant klebsiella pneumoniae during treatment via porin expression modification.klebsiella pneumoniae, an enterobacteriaceae that mostly causes hospital-acquired infections, belongs to the recently published who's list of antibiotic-resistant pathogens that pose the greatest threat to human health. indeed, k. pneumoniae is the enterobacterial species most concerned by both resistance to extended-spectrum cephalosporins, due to extended-spectrum β-lactamase (esbl) production, and resistance to carbapenems, i.e. the β-lactams with the broadest activity. carbapenem resistance ...201728751669
nosocomial bacterial infections and their antimicrobial susceptibility patterns among patients in ugandan intensive care units: a cross sectional study.the intensive care unit (icu) admits critically ill patients requiring advanced airway, respiratory, cardiac and renal support. despite the highly-specialized interventions, the mortality and morbidity is still high due to a number of reasons including nosocomial infections, which are the most likely complications in hospitalized patients with the rates being highest among icu patients.201728754148
cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects.oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. in this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis‑1-benzyl-n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans‑n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of bacillus cereus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, salmonella enterica, serratia mar ...201728755871
antibiofilm potential of synthetic 2-amino-5-chlorobenzophenone schiff bases and its confirmation through fluorescence microscopy.antibacterial/antibiofilm potential of microwave-assisted synthetic thirty-three 2-amino-5-chloro benzophenone schiff bases have been carried out against four bacterial strains i.e. klebsiella pneumoniae, proteus mirabilis, staphylococcus aureus and streptococcus mutans. among them compounds 5, 6, 8, 9, 14, 16, 22, 24, 26, and 30-32 showed antibiofilm activities against isolates at less than 100 μg/ml concentrations. these compounds showed enhanced antibiofilm activity against s. aureus as compa ...201728755874
retro analog concept: comparative study on physico-chemical and biological properties of selected antimicrobial peptides.increasing drug resistance of common pathogens urgently needs discovery of new effective molecules. antimicrobial peptides are believed to be one of the possible solutions of this problem. one of the approaches for improvement of biological properties is reversion of the sequence (retro analog concept). this research is based on investigation of antimicrobial activity against gram-positive, gram-negative bacteria, and fungi, hemolysis of erythrocytes, interpretation of the circular dichroism spe ...201728756544
risk factors and predictors of mortality of candidaemia among critically ill patients: role of antifungal prophylaxis in its development and in selection of non-albicans species.the aim of the present study is to identify risk factors for development and predictors of mortality of candidaemia among critically ill patients.201728756598
antioxidant and hepatoprotective potentials of novel endophytic fungus achaetomium sp., from euphorbia isolate, identify and evaluate the antioxidant, antimicrobial, hepatoprotective potentials, total phenolic content, flavonoid content, tannin content of ethyl acetate extract of endophytic fungus achaetomium sp., isolated from euphorbia hirta.201728756924
bacteriology of peritonsillar abscess: the changing trend and predisposing factors.peritonsillar abscess (pta) is the most common deep neck infection. the infectious microorganism may be different according to clinical factors.201728756939
invasive klebsiella pneumoniae liver abscess syndrome. 201728757731
production, characterization, and antimicrobial activity of mycocin produced by debaryomyces hansenii dsmz70238.the present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by d. hansenii dsmz70238 against several pathogenic microorganisms. in this study, the effects of nacl, ph, and temperature, killer toxin production, and antimicrobial activity were studied. the results showed that the optimum inhibitory effect of killer toxin was at 8% nacl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm for staphylococcus ...201728757872
active sites of o2-evolving chlorite dismutases probed by halides and hydroxides and new iron-ligand vibrational correlations.o2-evolving chlorite dismutases (clds) fall into two subfamilies, which efficiently convert clo2(-) to o2 and cl(-). the cld from dechloromonas aromatica (dacld) represents the chlorite-decomposing homopentameric enzymes found in perchlorate- and chlorate-respiring bacteria. the cld from the gram-negative human pathogen klebsiella pneumoniae (kpcld) is representative of the second subfamily, comprising homodimeric enzymes having truncated n-termini. here steric and nonbonding properties of the d ...201728758386
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