PMID(sorted ascending)
a carbon-dot-based fluorescent nanosensor for simple visualization of bacterial nucleic acids.a simple and facile method for sensing of nucleic acids is in great need for disease biomarker detection and diagnosis. herein, a fluorescent nanosensor utilizing carbon dot nanoparticles is introduced that form visible precipitates in the presence of target dna. carbon dot nanoparticles are fabricated by microwave pyrolysis of polyethylenimine, which emits strong photoluminescence and can form precipitates when added to target dna oligonucleotides. the precipitates can be easily visualized by u ...201728614623
intraventricular administration of tigecycline for the treatment of multidrug-resistant bacterial meningitis after craniotomy: a case report.intracranial infections, especially multidrug-resistant (mdr) bacterial meningitis, are one of the most severe complications after craniotomy and may greatly impact patient outcomes.201728614982
multi-drug resistant klebsiella pneumoniae strains circulating in hospital setting: whole-genome sequencing and bayesian phylogenetic analysis for outbreak investigations.carbapenems resistant enterobacteriaceae infections are increasing worldwide representing an emerging public health problem. the application of phylogenetic and phylodynamic analyses to bacterial whole genome sequencing (wgs) data have become essential in the epidemiological surveillance of multi-drug resistant nosocomial pathogens. between january 2012 and february 2013, twenty-one multi-drug resistant k. pneumoniae strains, were collected from patients hospitalized among different wards of the ...201728615687
serum inflammatory biomarkers and clinical outcomes of copd exacerbation caused by different pathogens.copd exacerbation is characterized by worsening of symptoms, warranting change in treatment. systemic and airway inflammation play roles in the pathogenesis of copd exacerbation. we hypothesized whether increased serum inflammatory biomarkers are associated with the clinical outcomes of copd exacerbation caused by different infectious pathogens.201728615935
demonstration that a klebsiella pneumoniae subsp. pneumoniae isolated from an insect (nezara viridula) harbors a plasmid-borne type iv secretion system.previously, we reported the isolation of klebsiella pneumoniae subspecies pneumoniae strain kp 5-1 from a southern green stink bug (nezara viridula) that is a significant pest of numerous economically important crops. we subsequently sequenced the strains whole genome. here, we report the presence of a functional plasmid-borne type iv secretion (tfss) system that was identified using genomic mining of the annotated genome. comparison of the kp 5-1 resident 186 kb plasmid (pkp 5-1) with nine othe ...201728616744
identification of pathogenic factors in klebsiella pneumoniae using impedimetric sensor equipped with biomimetic surfaces.k.pneumoniae is an opportunistic pathogen that causes nosocomial infections, such as, pneumonia, urinary tract infections, septic shock, and gastro intestinal disease. lipopolysaccharide (lps), capsular polysaccharide, and fimbriae are recognized major virulence factors of k.pneumoniae and play key roles during early stages of infections. in this study, we functionalized the surface of gold electrode with mannose and mucin to monitor the adhesion-associated virulence factors of k.pneumoniae. the ...201728617330
an unusual culprit: klebsiella pneumoniae causing septicaemia outbreaks in neonatal pigs?this study investigated the cause of recent outbreaks of septicaemia in neonatal pigs in australia (victoria and queensland). septicaemia in neonatal pigs is commonly caused by enterotoxigenic e. coli, extraintestinal pathogenic e. coli and beta-haemolytic streptococci. infrequent causes of septicaemia are actinobacillus suis and citrobacter freundii. therefore, it was quite unexpected when klebsiella pneumoniae was isolated in predominant growth from multiple organs of pigs dying of septicaemia ...201728619154
isolation, morphological identification and in vitro antibacterial activity of endophytic bacteria isolated from azadirachta indica (neem) leaves.the objective of this study was to isolate endophytic bacteria from azadirachta indica (neem) leaves, their identification and investigate their antibacterial activity against three gram-positive bacteria, staphylococcus aureus, streptococcus pyogenes and bacillus cereus and gram-negative bacteria escherichia coli, salmonella typhimurium and klebsiella pneumoniae.201728620254
spectrum and antibiotic resistance of uropathogens between 2004 and 2015 in a tertiary care hospital in hungary.surveillance of the bacterial spectrum and antibiotic-resistance patterns of locally occurring uropathogens is essential to serve as a basis for empirical treatment of urinary tract infections (utis), as antibiotic-resistance rates may vary geographically with significant differences between countries and regions, and with time.201728621642
clinical features and prognostic factors of klebsiella endophthalmitis-10-year experience in an endemic region.aimsto identify the clinical features and prognostic factors of endogenous endophthalmitis caused by klebsiella pneumoniae.methodsthis is a retrospective case series of all patients with klebsiella endophthalmitis managed from january 2006 to december 2015 by kowloon east ophthalmic service. statistical analysis involved hypothesis testing on the spss 18.0 software (spss). a significance level of p<0.05 was taken.resultsin the 10-year period, k. pneumoniae accounted for 19 out of 39 cases of end ...201728622312
acyloxyacyl hydrolase promotes the resolution of lipopolysaccharide-induced acute lung injury.pulmonary infection is the most common risk factor for acute lung injury (ali). innate immune responses induced by microbe-associated molecular pattern (mamp) molecules are essential for lung defense but can lead to tissue injury. little is known about how mamp molecules are degraded in the lung or how mamp degradation/inactivation helps prevent or ameliorate the harmful inflammation that produces ali. acyloxyacyl hydrolase (aoah) is a host lipase that inactivates gram-negative bacterial endotox ...201728622363
the bactericidal effect of simultaneous titanium oxide on common hospital bacteria.bacteria prevalence has increased in health centers and hospitals. infection control can decrease the number of bacteria. bacteria cause 90% of nosocomial infections and fungi, viruses, or protozoa are less involved. bacteria control can decrease the number of diseases. the simultaneous titanium oxide (smtio) is a new method of disinfection. this process degrades bacteria by producing hydroxyl radical. the present study was conducted with the aim of analyzing the bactericidal effect of simultane ...201728623574
[causative bacterial strains and sensitivityto antimicrobial agents in female acute uncomplicated cystitis].recently, wide spreading of fluoloquinolone resistant escherichia coli is a serious problem inthe treatment of urinary tract infection. to investigate the causative bacterial strains of female acute uncomplicated cystitis (auc) in the community and their sensitivity to antimicrobial agents, we retrospectively reviewed the medical records of 215 female auc patients treated at our clinics from april 2014 to june 2015. two hundred and nineteen strains were isolated as the causative bacteria from th ...201728625025
strain-level metagenomic analysis of the fermented dairy beverage nunu highlights potential food safety risks.the rapid detection of pathogenic strains in food products is essential for the prevention of disease outbreaks. it has already been demonstrated that whole-metagenome shotgun sequencing can be used to detect pathogens in food but, until recently, strain-level detection of pathogens has relied on whole-metagenome assembly, which is a computationally demanding process. here we demonstrated that three short-read-alignment-based methods, i.e., metamlst, panphlan, and strainphlan, could accurately a ...201728625983
resistance to colistin in klebsiella pneumoniae: a 4.0 strain?the global rise of multidrug-resistant gram-negative bacteria represents an increasing threat to patient safety. from the first observation of a carbapenem-resistant gram-negative bacteria a global spread of extended-spectrum beta-lactamases and carbapenemases producing klebsiella pneumoniae has been observed. treatment options for multidrug-resistant k. pneumoniae are actually limited to combination therapy with some aminoglycosides, tigecycline and to older antimicrobial agents. unfortunately, ...201728626539
optimization of tannase production by a novel klebsiella pneumoniae kp715242 using central composite design.a novel tannase producing bacterial strain was isolated from rhizospheric soil of acacia species and identified as klebsiella pneumoniae kp715242. a 3.25-fold increase in tannase production was achieved upon optimization with central composite design using response surface methodology. four variables namely ph, temperature, incubation period, and agitation speed were used to optimize significant correlation between the effects of these variables on tannase production. a second-order polynomial w ...201528626722
colistin resistance associated with outer membrane protein change in klebsiella pneumoniae and enterobacter this study, outer membrane proteins (omps) of colistin-resistant klebsiella pneumoniae and enterobacter asburiae were analyzed. one colistin-susceptible and three colistin-resistant k. pneumoniae sequence type 258 strains as well as one colistin-susceptible e. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. omp analysis of each strain was performed by microchip method. matrix-assisted laser desorption ionization time of flight/mass spectrometry (maldi- ...201728629229
molecular characterization of clinical imp-producing klebsiella pneumoniae isolates from a chinese tertiary hospital.imp-producing klebsiella pneumoniae (impkpn) exhibits sporadic prevalence in china. the mechanisms related to the spread of impkpn remain unclear.201728629366
antibiotic resistance determinants and clonal relationships among multidrug-resistant isolates of klebsiella the present study, we performed pcr based screening to determine the presence of antibiotic resistance genes and sequencing to find mutation in qrdr region among fourteen isolates of k. pneumoniae. association analysis was conducted to detect the co-resistance among the isolates. multi-locus sequence analysis was carried out to determine the clonal relationship among them. all the k. pneumoniae isolates showed resistance to multiple antibiotics and exhibited cross-resistance to antibiotics. a ...201728629728
variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons.polyhydroxyalkanoates (pha) are efficient, renewable and environment friendly polymeric esters. these polymers are synthesized by a variety of microbes under stress conditions. this study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. six bacterial strains were isolated and identified as bacillus cereus (kf270349), klebsiella pneumoniae (kf270350), bacillus subtilis (kf270351), brevibacterium halotolerance (kf270352) ...201728629970
efficacy of cefiderocol against carbapenem-resistant gram-negative bacilli in immunocompetent-rat respiratory tract infection models recreating human plasma pharmacokinetics.cefiderocol (s-649266), a novel siderophore cephalosporin, shows potent activity against carbapenem-resistant gram-negative bacilli. in this study, we evaluated the efficacy of cefiderocol against carbapenem-resistant gram-negative bacilli (pseudomonas aeruginosa, acinetobacter baumannii, and klebsiella pneumoniae) in immunocompetent-rat respiratory tract infection models recreating plasma pharmacokinetics (pk) profiles in healthy human subjects. a total of 6 clinical isolates (1 cephalosporin-s ...201728630178
identification of 1-((2,4-dichlorophenethyl)amino)-3-phenoxypropan-2-ol, a novel antibacterial compound active against persisters of pseudomonas aeruginosa.antibiotics typically fail to completely eradicate a bacterial population, leaving a small fraction of transiently antibiotic-tolerant persister cells intact. persisters are therefore seen to be a major cause of treatment failure and greatly contribute to the recalcitrant nature of chronic infections. the current study focused on pseudomonas aeruginosa, a gram-negative pathogen belonging to the notorious eskape group of pathogens (enterococcus faecium, staphylococcus aureus, klebsiella pneumonia ...201728630188
identifying spectra of activity and therapeutic niches for ceftazidime-avibactam and imipenem-relebactam against carbapenem-resistant enterobacteriaceae.we determined imipenem, imipenem-relebactam, ceftazidime, and ceftazidime-avibactam mics against 100 cre isolates that underwent whole-genome sequencing. klebsiella pneumoniae carbapenemases (kpcs) were the most common carbapenemases. forty-six isolates carried extended-spectrum β-lactamases (esbls). with the addition of relebactam, imipenem susceptibility increased from 8% to 88%. with the addition of avibactam, ceftazidime susceptibility increased from 0% to 85%. neither imipenem-relebactam no ...201728630202
fecal carriage of extended-spectrum β-lactamase- and carbapenemase-producing enterobacteriaceae in egyptian patients with community-onset gastrointestinal complaints: a hospital -based cross-sectional study.the aim of this study was to determine the prevalence of extended-spectrum β-lactamase (esbl) and carbapenemase production among enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to el-ahrar general hospital, zagazig, egypt in the period between january 2013 and may 2013.201728630686
emerging antimicrobial resistance in early and late-onset neonatal sepsis.compared to developed countries, the use of antimicrobials in egypt is less regulated and is available over the counter without the need for prescriptions. the impact of such policy on antimicrobial resistance has not been studied. this study aimed to determine the prevalence of early and late onset sepsis, and the frequency of antimicrobial resistance in a major referral neonatal intensive care unit (nicu).201728630687
mait cells launch a rapid, robust and distinct hyperinflammatory response to bacterial superantigens and quickly acquire an anergic phenotype that impedes their cognate antimicrobial function: defining a novel mechanism of superantigen-induced immunopathology and immunosuppression.superantigens (sags) are potent exotoxins secreted by staphylococcus aureus and streptococcus pyogenes. they target a large fraction of t cell pools to set in motion a "cytokine storm" with severe and sometimes life-threatening consequences typically encountered in toxic shock syndrome (tss). given the rapidity with which tss develops, designing timely and truly targeted therapies for this syndrome requires identification of key mediators of the cytokine storm's initial wave. equally important, ...201728632753
high-throughput identification and rational design of synergistic small-molecule pairs for combating and bypassing antibiotic resistance.antibiotic-resistant infections kill approximately 23,000 people and cost $20,000,000,000 each year in the united states alone despite the widespread use of small-molecule antimicrobial combination therapy. antibiotic combinations typically have an additive effect: the efficacy of the combination matches the sum of the efficacies of each antibiotic when used alone. small molecules can also act synergistically when the efficacy of the combination is greater than the additive efficacy. however, sy ...201728632788
sirtuin 3 deficiency does not alter host defenses against bacterial and fungal infections.sirtuin 3 (sirt3) is the main mitochondrial deacetylase. sirt3 regulates cell metabolism and redox homeostasis, and protects from aging and age-associated pathologies. sirt3 may drive both oncogenic and tumor-suppressive effects. sirt3 deficiency has been reported to promote chronic inflammation-related disorders, but whether sirt3 impacts on innate immune responses and host defenses against infections remains essentially unknown. this aspect is of primary importance considering the great intere ...201728634345
genomic and phenotypic characterisation of fluoroquinolone resistance mechanisms in enterobacteriaceae in durban, south africa.resistance to fluoroquinolones (fq) is being increasingly reported and found to be mediated by efflux pumps, plasmid-mediated quinolone resistance genes (pmqr) and mutations in gyra, gyrb, parc and pare. however, studies reporting on fq resistance mechanisms (fqrm), particularly in africa, are focused mostly on salmonella. this study used a whole-genome-based approach to describe fqrm in forty-eight clinical enterobacteriaceae isolates comprising of klebsiella pneumoniae (n = 21), serratia marce ...201728636609
molecular and microbiological report of a hospital outbreak of ndm-1-carrying enterobacteriaceae in characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant enterobacteriaceae (cre) in a tertiary-care hospital in mexico.201728636666
deleting pck improves growth and suppresses by-product formation during 1,3-propanediol fermentation by klebsiella investigate the role of phosphoenolpyruvate (pep) carboxylation in cell metabolism in klebsiella pneumoniae.201728637087
emergence of ceftazidime/avibactam non-susceptibility in an mdr klebsiella pneumoniae isolate.avibactam is a novel broad-range β-lactamase inhibitor active against ambler class a (including esbl and kpc) and some ambler class c and d (e.g. oxa-48) enzymes. we here report on the emergence of ceftazidime/avibactam resistance in clinical, multiresistant, oxa-48 and ctx-m-14-producing klebsiella pneumoniae isolate dt12 during ceftazidime/avibactam treatment.201728637339
evaluation of microorganisms causing ventilator-associated pneumonia in a pediatric intensive care unit.the aim of this study was to identify microorganisms causing ventilator-associated pneumonia (vap) and also study the antibiotic resistance/susceptibility.201728638248
molecular identification of aminoglycoside-modifying enzymes and plasmid-mediated quinolone resistance genes among klebsiella pneumoniae clinical isolates recovered from egyptian patients.inappropriate use of antibiotics in clinical settings is thought to have led to the global emergence and spread of multidrug-resistant pathogens. the goal of this study was to investigate the prevalence of genes encoding aminoglycoside resistance and plasmid-mediated quinolone resistance among clinical isolates of klebsiella pneumoniae. all k. pneumoniae isolates were phenotypically identified using api 20e and then confirmed genotypically through amplification of the specific k. pneumoniae phoe ...201728638412
lipid peroxidation in gram-negative bacteremia modulates the risk for septic shock and infections by resistant klebsiella pneumoniae.controversies in outcomes with the parenteral administration of antioxidants as adjuvant therapies led to the measurement of malondialdehyde (mda), a product of lipid peroxidation, in serum collected from 120 patients with primary gram-negative bacteremia during the first 24 h from sepsis onset. mda was measured by the thiobarbiturate assay, followed by high-performance liquid chromatography (hplc) analysis. after receiver operator characteristic (roc) curve analysis, patients were divided into ...201728639164
molecular characterization of intestinal carriage of carbapenem-resistant enterobacteriaceae among inpatients at two iranian university hospitals: first report of co-production of bla ndm-7 and bla oxa-48.gastrointestinal colonization of carbapenem-resistant enterobacteriaceae (cre) could serve as a reservoir for the transmission of these pathogens in the clinical setting. the aim of this study was to investigate the intestinal carriage of cre and to analyze risk factors for cre carriage. rectal swabs were collected from 95 patients at two iranian university hospitals. cre screening was performed using selective media (chromagar and macconkey agar). polymerase chain reaction (pcr) was used to det ...201728639165
first description of methyltransferases in extensively drug-resistant klebsiella pneumoniae isolates from saudi arabia.the resistance determinants for carbapenems, fluoroquinolones and aminoglycosides were characterized in 16 extensively drug-resistant klebsiella pneumoniae (xdrkpn) strains collected from saudi hospitals during 2014.201728639934
synthesis and evaluation of arylamidine derivatives for new antimicrobial and cytotoxic activities.a series of arylamidines 3a-j was designed, synthesized and investigated for antimicrobial activity. structures of the compounds were confirmed by ir, 1h-nmr and 13c-nmr and a 2d spectroscopic study was performed. a preliminary screening of the antimicrobial tests clearly showed that three out of ten arylamidines, viz, 3f, 3g and 3i, were effective against all the gram-negative bacteria: klebsiella pneumoniae, pseudomonas aeruginosa and salmonella enteric; and against the yeast, candida albicans ...201728640352
1,1ꞌ-carbonyldiimidazole (cdi) mediated facile synthesis, structural characterization, antimicrobial activity and in-silico studies of coumarin-3-carboxamide derivatives.despite of the availability of variety of antibacterial agents, re-emergance of pathogenic bacteria is still a serious medical concern. so, identification of new, safer, and selective antibacterial agents is the key interest in the medicinal chemistry research.201728641525
the antimicrobial spectrum of xeroform(®).xeroform(®) is a petrolatum-based fine mesh gauze containing 3% bismuth tribromophenate. bismuth, similar to other metals, has antimicrobial properties. xeroform(®) has been used for decades in burn and plastic surgery as a donor site dressing and as a covering for wounds or partial thickness burns. despite this, the antimicrobial spectrum of xeroform(®) remains largely unknown. we examined the in-vitro efficacy of xeroform(®) against common burn pathogens using zone-of-inhibition methodology in ...201728641915
clinical characteristics and epidemiology of sepsis in the neonatal intensive care unit in the era of multi-drug resistant organisms: a retrospective review.sepsis in the neonatal intensive care unit (nicu) remains one of the most significant causes of morbidity and mortality, especially for preterm newborns. multi-drug resistant organisms (mdros) are emerging as important pathogens that cause neonatal sepsis in nicu. therefore, studying the epidemiology, clinical features, and outcome caused by mdros vs. non-mdros, and identifying risk factors that may predispose patients to sepsis by mdros are important.201728642139
epidemiology and outcomes of bloodstream infections in 177 severe burn patients from an industrial disaster: a multicentre retrospective determine the characteristics of bloodstream infections (bsis) and to evaluate the impact of bsis on mortality in severe burn patients.201728642142
nonribosomal peptide synthetase biosynthetic clusters of eskape pathogens.covering: up to 2017.natural products are important secondary metabolites produced by bacterial and fungal species that play important roles in cellular growth and signaling, nutrient acquisition, intra- and interspecies communication, and virulence. a subset of natural products is produced by nonribosomal peptide synthetases (nrpss), a family of large, modular enzymes that function in an assembly line fashion. because of the pharmaceutical activity of many nrps products, much effort has gone in ...201728642945
impact of appropriateness of empiric therapy on outcomes in community-onset bacteremia by extended-spectrum-β-lactamase producing escherichia coli and klebisella pneumoniae definitively treated with carbapenems.despite a significant increase of bloodstream infection caused by extended-spectrum-β-lactamase (esbl)-producing enterobacteriaceae in the community-setting, information regarding clinical outcomes of inappropriate empiric therapy (iat) in patients with those infections is limited. a multicenter-retrospective cohort study was conducted in four hospitals. a total of 249 adults were identified to have community-onset bacteremia caused by esbl-producing escherichia coli and klebsiella pneumoniae, a ...201728643188
multidrug resistance mechanisms of carbapenem resistant klebsiella pneumoniae strains isolated in chongqing, china.carbapenem-resistant klebsiella pneumoniae (crkp) is considered a serious global threat. however, little is known regarding the multidrug resistance (mdr) mechanisms of crkp. this study investigated the phenotypes and mdr mechanisms of crkp and identified their clonal characteristics.201728643488
higher mortality of severe influenza patients with probable aspergillosis than those with and without other coinfections.aspergillus-associated infection might comprise up to 23-29% of severe influenza patients from the community throughout stay in an intensive care unit (icu). in taiwan, cases of severe influenza with aspergillosis are increasingly reported. therefore, we describe the relative risk of mortality among severe influenza patients with aspergillosis and other coinfections compared to severe influenza patients without aspergillus coinfections.201728647219
extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates from neonates in tunisia.this study was conducted to investigate extended-spectrum-β-lactamase (esbl) producing enterobacteriaceae isolates from the center of maternity and neonatology of monastir, tunisia. fourty-six strains out of 283 were found to produce esbl: klebsiella pneumoniae (n = 37), escherichia coli (n = 6), enterobacter cloacae (n = 2), and citrobacter freundi (n = 1). genotyping analysis, using eric2 and rapd, showed that strains were clonally unrelated. pcr amplification followed by sequencing revealed t ...201728648624
in vitro antibacterial activity of fosfomycin combined with other antimicrobials against kpc-producing klebsiella pneumoniae.the increasing prevalence of kpc-producing klebsiella pneumoniae (kpc-kp) strains poses a serious threat to patients. therapeutic options are limited to colistin, fosfomycin, tigecycline and selected aminoglycosides. although the combination of fosfomycin with other antimicrobials is recommended, data regarding possible synergistic activity in vitro and in vivo appear inconsistent. here we report that five drug combinations (fosfomycin combined with imipenem, ertapenem, tigecycline, colistin or ...201728648647
multidrug-resistant enterobacteriaceae in lebanese hospital wastewater: implication in the one health concept.extended-spectrum beta-lactamases (esbl) and carbapenemase-producing organisms pose severe problems for hospitalized patients. in the absence of efficient sanitation and sewage disposal, the risks for transmission of hospital organisms into the community are high. our objectives were to study the occurrence and mechanisms of resistance of multidrug-resistant gram-negative bacilli in two lebanese hospital sewage treatment plants. wastewater samples were collected, filtered, and cultivated on macc ...201728650688
complete dna sequence of an incm1 plasmid bearing the novel qnre1 plasmid-mediated quinolone resistance variant and blactx-m-8 from klebsiella pneumoniae sequence type 147. 201728652231
stand-alone eal domain proteins form a distinct subclass of eal proteins involved in regulation of cell motility and biofilm formation in enterobacteria.the second messenger cyclic dimeric gmp (c-di-gmp) is almost ubiquitous among bacteria as are the c-di-gmp turnover proteins, which mediate the transition between motility and sessility. eal domain proteins have been characterized as c-di-gmp-specific phosphodiesterases. while most eal domain proteins contain additional, usually n-terminal, domains, there is a distinct family of proteins with stand-alone eal domains, exemplified by salmonella enterica serovar typhimurium proteins stm3611 (yhjh/p ...201728652301
identification and characterization of two klebsiella pneumoniae lpxl lipid a late acyltransferases and their role in virulence.klebsiella pneumoniae causes a wide range of infections, from urinary tract infections to pneumonia. the lipopolysaccharide is a virulence factor of this pathogen, although there are gaps in our understanding of its biosynthesis. here we report on the characterization of k. pneumoniaelpxl, which encodes one of the enzymes responsible for the late secondary acylation of immature lipid a molecules. analysis of the available k. pneumoniae genomes revealed that this pathogen's genome encodes two ort ...201728652313
biological activities of allium sativum and zingiber officinale extracts on clinically important bacterial pathogens, their phytochemical and ft-ir spectroscopic analysis.the spread of bacterial infectious diseases is a major public threat. herbs and spices have offered an excellent, important and useful source of antimicrobial agents against many pathological infections. in the current study, the antimicrobial potency of fresh, naturally and commercial dried allium sativum and zingiber officinale extracts had been investigated against seven local clinical bacterial isolates such as escherichia coli, pseudomonas aeruginosa, klebsiella pneumonia, staphylococcus au ...201728653916
screening of leaves extracts from calamus aromaticus for their antimicrobial activity by disc diffusion assay.the current research investigates the anti-microbial activities of different solvent extracted samples from the leaves of calamus aromaticus against gram positive, gram negative bacteria and fungi using 500, 1000 and 2000 μg disc(-1) concentrations. escherichia coli, citorbacter freundii and candida albicans showed resistivity to crude methanolic extract and the same microbes were more susceptible to water extracted fractions. maximum activity was measured by hexane extracted fractions against p ...201728653924
identification and growth optimization of a marine bacillus dk1-sa11 having potential of producing broad spectrum antimicrobial compounds.control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. marine bacteria are an enormous source of bio-controlling agents. the aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain dk1-sa11 isolated from qingdao bay of china yellow sea. microscopic characterization, api® 20e and 50 chb kit base carbohy ...201728653930
novel plasmid-mediated colistin resistance gene mcr-3 in escherichia coli.the mobile colistin resistance gene mcr-1 has attracted global attention, as it heralds the breach of polymyxins, one of the last-resort antibiotics for the treatment of severe clinical infections caused by multidrug-resistant gram-negative bacteria. to date, six slightly different variants of mcr-1, and a second mobile colistin resistance gene, mcr-2, have been reported or annotated in the genbank database. here, we characterized a third mobile colistin resistance gene, mcr-3 the gene coexisted ...201728655818
metastatic endophthalmitis and thyroid abscess complicating klebsiella pneumoniae liver abscess.the thyroid is resistant to infection due to its anatomical and physiological characteristics. we present a rare case of invasive liver abscess with metastatic thyroid abscess and endogenous endophthalmitis in a previously healthy 55-year-old female patient without diabetes or other medical illness. this report raises an important question of the virulence of klebsiella pneumoniae as an increasingly common causative agent of liver abscess.201728656747
propensity score matched analysis comparing the clinical outcome of klebsiella pneumoniae and escherichia coli causing community-onset monomicrobial bacteremia.bacteremia is a life-threatening condition that is associated with substantial healthcare costs. escherichia coli and klebsiella pneumoniae are the leading causes of community-onset gram-negative bacteremia. however, a comprehensive comparison between these pathogens involved in bacteremia episodes has yet to be this retrospective cohort study, adults with community-onset monomicrobial bacteremia caused by e coli or k pneumoniae were recruited in the emergency department of a medical ...201728658101
molecular epidemiology and risk factors of carbapenem-resistant klebsiella pneumoniae infections in eastern china.background: the increasing prevalence of carbapenem-resistant klebsiella pneumoniae (crkp) poses an immediate threat to treatment worldwide. this retrospective study assessed the molecular epidemiology and determined the risk factors for and outcomes of crkp infections in a general teaching hospital in shanghai, china. methods: from january 2013 to july 2015, 100 consecutive unique crkp isolates isolated from hospitalized patients were collected. isolates were screened for antibiotic resistance ...201728659886
successful treatment of multidrug resistant klebsiella pneumoniae using dual carbapenem regimen in immunocompromised patient.this report describes a longitudinal case of immunocompromised patient post kidney transplant who was admitted to our institution repeatedly for treatment of various infections caused by multi-drug resistant klebsiella pneumoniae. the patient was successfully treated with a combination of ertapenem/meropenem on multiple occasions despite the elevated mics. our observations corroborate previous preclinical studies and case reports showing the efficacy of double carbapenem regimens against resista ...201728660131
three new abietane-type diterpenoids from plectranthus africanus and their antibacterial activities.three new abietane-type diterpenoids, plectranthroyleanones a - c (1 - 3), together with five known compounds (4 - 8) were isolated from the methanol extract of the whole plant of plectranthus africanus using column chromatography techniques. the structures of the new compounds were elucidated using a combination of 1d and 2d nmr and hresims analyses. compound 1 exhibited weak activities with minimal inhibitory concentration values of 75 µg/ml against gram-positive bacteria, bacillus subtilis an ...201728662528
urinary tract infections after kidney transplantation: a risk factor analysis of 417 patients.background the aim of this study was to evaluate the number of urinary tract infections (uti) that occur after kidney transplantation (kt) and to identify possible risk factors for development of a uti. material and methods we retrospectively analyzed all kts performed between january 2012 and december 2013 in the erasmus university medical center, rotterdam. uti was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacteriu ...201728663538
monitoring in vitro antibacterial efficacy of 26 indian spices against multidrug resistant urinary tract infecting screen methanolic extracts of 26 commonly used indian spices against nine species of uropathogenic bacteria (enterococcus faecalis, staphylococcus aureus, acinetobacter baumannii, citrobacter freundii, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa), isolated from clinical samples of a tertiary care hospital for antibacterial activity.201428664089
antimicrobial and cytotoxic evaluation of some herbal essential oils in comparison with common antibiotics in bioassay condition.since ancient times, various infectious diseases have been treated using herbal drugs. today, efforts regarding the discovery of the effectual components of plants possessing antimicrobial properties are advanced. herbal essential oils are widely used for treatment of various diseases, and they play an important role in health care considerations.201428664090
does acinetobacter baumannii serve as a source for blandm dissemination into enterobacteriaceae in hospitalized patients?the goal was to study the possibility that acinetobacter baumannii serve as an epidemiologically significant source for transmission of the blandm gene to enterobacteriaceae by horizontal gene transfer (hgt) in hospitalized patients. the study was done at the tel-aviv sourasky medical center from december 2014 until august 2015. clinical and surveillance rectal cultures were collected as per hospital policies and were analyzed for the presence of new delhi metallo-beta-lactamase-producing entero ...201728665176
klebsiella pneumoniae. 201728665914
epidemiology, management, and outcome of carbapenem-resistant klebsiella pneumoniae bloodstream infections in hospitals within the same endemic metropolitan the last decade, carbapenem-resistant klebsiella pneumoniae (cr-kp) has become endemic in several countries, including italy. in the present study, we assessed the differences in epidemiology, management, and mortality of cr-kp bloodstream infection (bsi) in the three main adult acute-care hospitals of the metropolitan area of genoa, italy. from january 2013 to december 2014, all patients with cr-kp bsi were identified through the computerized microbiology laboratory databases of the three ho ...201728668656
in vivo development of tigecycline resistance in klebsiella pneumoniae owing to deletion of the ramr ribosomal binding site.tigecycline resistance is emerging among klebsiella pneumoniae, but knowledge regarding in vivo development of tigecycline resistance is limited. here we report a new mechanism of tigecycline resistance in k. pneumoniae that evolved during tigecycline therapy. klebsiella pneumoniae isolates were consecutively obtained from urine samples of a patient with scrotal abscess and urinary tract infection before and during tigecycline treatment. two tigecycline-resistant k. pneumoniae strains (kp-3r and ...201728668690
wastewater is a reservoir for clinically relevant carbapenemase- and 16s rrna methylase-producing enterobacteriaceae.the aim of this study was to evaluate wastewater for carbapenemase-producing enterobacteriaceae (cpe) and 16s rrna methylase-producing gram-negative bacteria (mpb) and to assess their occurrence following wastewater treatment. wastewater samples were collected between june 2015 and march 2016 in the sewage network of the city of basel (switzerland) from sites located before and after influx of wastewater from the hospital into the sewage network. samples were also obtained from the influent and ...201728668692
molecular epidemiology of colistin-resistant enterobacteriaceae in inpatients and avian from china: high prevalence of mcr-negative klebsiella pneumoniae.whether chromosomal and transmissible mechanisms contribute simultaneously to colistin resistance in klebsiella pneumoniae and escherichia coli remains unknown. this study aims to identify the underlying mechanisms of colistin-resistance in inpatient and avian k. pneumoniae and e. coli in china. we retrospectively screened 2353 enterobacteriaceae isolates from inpatients at multi-centers during 2011-2014, and 168 avian isolates from one slaughterhouse in 2013 for the presence of mcr-1/mcr-2. mut ...201728668693
nationwide surveillance of bacterial respiratory pathogens conducted by the surveillance committee of japanese society of chemotherapy, the japanese association for infectious diseases, and the japanese society for clinical microbiology in 2012: general view of the pathogens' antibacterial susceptibility.the nationwide surveillance on antimicrobial susceptibility of bacterial respiratory pathogens from the patients in japan was conducted by japanese society of chemotherapy, japanese association for infectious diseases and japanese society for clinical microbiology in 2012. the isolates were collected from clinical specimens obtained from well-diagnosed adult patients with respiratory tract infections during the period between january and december in 2012 by three societies. antimicrobial suscept ...201728669567
quick 96fasp for high throughput quantitative proteome analysis.filter aided sample preparation (fasp) is becoming a central method for proteomic sample cleanup and peptide generation prior to lc-ms analysis. we previously adapted this method to a 96-well filter plate, and applied to prepare protein digests from cell lysate and body fluid samples in a high throughput quantitative manner. while the 96fasp approach is scalable and can handle multiple samples simultaneously, two key advantages compared to single fasp, it is also time-consuming. the centrifugati ...201728669814
inhaled colistin monotherapy for respiratory tract infections in adults without cystic fibrosis: a systematic review and meta-analysis.inhaled colistin is becoming increasingly popular against respiratory tract infections due to multidrug resistant (mdr) gram-negative bacteria since it may overcome the problems associated with iv administration.201728669836
a novel approach for combating klebsiella pneumoniae biofilm using histidine functionalized silver nanoparticles.treating pathogens is becoming challenging because of multidrug resistance and availability of limited alternative therapies which has further confounded this problem. the situation becomes more alarming when multidrug resistant pathogens form a 3d structure known as biofilm. biofilms are formed in most of the infections especially in chronic infections where it is difficult to eradicate them by conventional antibiotic therapy. chemically synthesized nanoparticles are known to have antibiofilm a ...201728670301
molecular characterization of klebsiella pneumoniae isolates from stool specimens of outpatients in sentinel hospitals beijing, china, 2010-2015.klebsiella pneumoniae (k. pneumoniae) is an opportunistic pathogen associated with community-acquired infections and nosocomial infections. from 2010 to 2015, k. pneumoniae testing was included into the exiting diarrhea-syndrome surveillance with objective to estimate the prevalence of k. pneumoniae in diarrhea-syndrome patients, test antibiotics susceptibility and investigate molecular characteristics.201728670346
in vitro pharmacokinetics/pharmacodynamics evaluation of fosfomycin combined with amikacin or colistin against kpc2-producing klebsiella pneumoniae.objectives: the emergence of carbapenem-resistant enterobacteriaceae, especially klebsiella pneumoniae, has become a major concern in clinic settings. combination therapy is gaining momentum to counter the secondary resistance and potential suboptimal efficacy of monotherapy. the aim of this study was to evaluate the bactericidal effect of fosfomycin (fm), amikacin (amk), or colistin (col) alone and combinations against kpc2-producing k. pneumoniae using dynamic model by simulating human pharmac ...201728670570
extended-spectrum beta-lactamase producing gram negative bacteria in iran: a review.the emergence and spread of extended spectrum β-lactamase (esbl)-producing gram- negative bacteria (gnb), particularly in enterobacteriaceae, acinetobacter baumannii, and pseudomonas aeruginosa, have increased all over the world. esbls are characterized by their ability to hydrolyze β-lactams, early cephalosporins, oxyimino-thiazolyl cephalosporins, and monobactams, but not cephamycins or carbapenems. the rate of nosocomial infections caused by esbl-producing gnb in asia pacific has increased an ...201728670639
carbapenem resistant klebsiella pneumoniae isolated from bloodstream infection: indian experience.increased incidence of multidrug resistant (mdr) gram negative infection has resulted in high rates of morbidity and mortality. klebsiella pneumoniae is one of the commonest mdr pathogens causing bacteraemia with limited therapeutic options such as colistin and tigecycline. present study focused on molecular characterisation of mdr k. pneumoniae from bloodstream infection and their clinical outcome. a total of 115 k. pneumoniae from january 2015 to september 2016 were included in the study which ...201728670975
targeted surveillance and infection-related risk factors of nosocomial infection in patients after neurosurgical operation.the purpose of this study was to investigate the risk factors of nosocomial infection in patients after neurosurgical operations, so as to provide reasonable, effective preventative measures in the future. a total of 1,600 patients with brain tumors, hydrocephalus, craniocerebral trauma and vascular disease treated in the neurosurgery room were chosen for targeted surveillance; and through analysis on the clinical data of patients, the incidence of nosocomial infection, the site of infection and ...201728671080
emerging trends of nosocomial pneumonia in intensive care unit of a tertiary care public teaching hospital in western india.nosocomial pneumonia poses great challenge to an intensivist. detailed information about hospital-acquired pneumonia (hap) and ventilator-acquired pneumonia (vap) is crucial for prevention and optimal management, thus improving quality intensive care unit (icu) care. hence, we aimed to study the current trend of nosocomial pneumonia in icu.201728671150
the distribution of carbapenem- and colistin-resistance in gram-negative bacteria from the tamil nadu region in india.the occurrence of carbapenem- and colistin-resistance among gram-negative bacteria is increasing worldwide. the aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in tamil nadu, india.201728671537
antibacterial activities of pyrenylated coumarins from the roots of prangos hulusii.the dichloromethane extract of the roots of prangos hulusii, a recently described endemic species from turkey, has yielded nine known and one new prenylated coumarins. the structures were elucidated by spectroscopic methods and direct comparison with the reference compounds where available. the root extract and its prenylated coumarins exhibit antimicrobial activity against nine standard and six clinically isolated strains at a concentration between 5 and 125 µg/ml. in particular, the new coumar ...201728671568
current problematic and emergence of carbapenemase-producing bacteria: a brief report from a libyan hospital.a collection of 94 gram-negative bacteria isolates, showing different antimicrobial resistance phenotypes including to the carbapenem classes was investigated. strains were originated form clinical sources from a single hospital in tripoli, libya during 2015 and were identified based on cultural and phenotypic characteristics, and fully characterized by the vitek automated system. forty-eight percent (48%) of the collection was identified as acinetobacter baumannii, 50% klebsiella pneumoniae and ...201728674573
titanium surfaces immobilized with the major antimicrobial fragment fk-16 of human cathelicidin ll-37 are potent against multiple antibiotic-resistant bacteria.infections on implanted medical devices are a challenging problem, especially when bacteria form difficult-to-treat biofilms. antimicrobial peptides are considered to be a solution due to their potency against antibiotic-resistant superbugs. previously, the authors' laboratory demonstrated the prevention of staphylococcal biofilm formation in an animal catheter model by injecting merecidin (formerly known as 17biphe2), a peptide engineered based on the only human cathelicidin. this study documen ...201728675109
dissemination of carbapenem-resistant klebsiella pneumoniae clinical isolates with various combinations of carbapenemases (kpc-2, ndm-1, ndm-4, and oxa-48) and 16s rrna methylases (rmtb and rmtc) in vietnam.twenty-seven clinical isolates of carbapenem-resistant klebsiella pneumoniae with mics ≥4 mg/l for imipenem or meropenem were obtained from inpatients in a hospital in vietnam. antimicrobial susceptibility tests and whole genome sequencing were performed. multilocus sequence typing and the presence of drug resistant genes were determined and a maximum-likelihood phylogenetic tree was constructed by snp alignment of whole genome sequencing data.201728676118
effect of the meropenem mic on the killing activity of meropenem and polymyxin b in combination against kpc-producing klebsiella pneumoniae. 201728676713
double carbapenem as a rescue strategy for the treatment of severe carbapenemase-producing klebsiella pneumoniae infections: a two-center, matched case-control study.recent reports have suggested the efficacy of a double carbapenem (dc) combination, including ertapenem, for the treatment of carbapenem-resistant klebsiella pneumoniae (cr-kp) infections. we aimed to evaluate the clinical impact of such a regimen in critically ill patients.201728679413
the respiratory threat posed by multidrug resistant gram-negative bacteria.respiratory infections are a major cause of global mortality and morbidity. in recent years, an increased incidence of multidrug-resistant (mdr) gram-negative bacteria (gnb) has been described. microorganisms such as pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae or acinetobacter baumannii have been identified as causative pathogens of different respiratory tract infections. several studies have detected mdr-gnb in patients with community-acquired and nosocomial pneumonia. furth ...201728681941
pneumonia in the developing world: characteristic features and approach to pneumonia (cap) is a common cause of morbidity and mortality in adults worldwide, but its epidemiology varies markedly by region. whilst in high-income countries, the predominant burden of cap is in the elderly and those with chronic cardiovascular and pulmonary co-morbidity, cap patients in low-income settings are often of working age and, in sub-saharan africa, frequently hiv-positive. although region-specific aetiological data are limited, they are sufficient to highlight m ...201728681972
complete genome sequence of a multidrug-resistant, blandm-1-expressing klebsiella pneumoniae k66-45 clinical isolate from norway.multidrug-resistant klebsiella pneumoniae is a major cause of hospital-acquired infections. here, we report the complete genome sequence of the multidrug-resistant, blandm-1-positive strain k. pneumoniae k66-45, isolated from a hospitalized norwegian patient.201728684580
draft genome sequence of a multidrug-resistant sequence type 231 outbreak-associated clone of klebsiella pneumoniae, kp41-2015, producing oxa-232 carbapenemase.carbapenem-resistant klebsiella pneumoniae infection is a rising public health threat due to limited therapeutic options. here, we report the genome sequence of a multidrug-resistant k. pneumoniae sequence type 231 (st231) strain associated with an outbreak of infections in an intensive care unit that carries a unique complement of resistance determinants.201728684582
association between virulence factors and extended spectrum beta-lactamase producing klebsiella pneumoniae compared to nonproducing isolates.klebsiella pneumoniae is considered an important opportunistic multidrug-resistant pathogen. extended spectrum β-lactamases (esbls) and expression of a multitude of virulence factors may work in a harmony resulting in treatment failure. this study was undertaken to compare the virulence characteristics and genetic relatedness between esbl and non-esbl producing k. pneumoniae. methods. antibiotic sensitivity test of all isolates was determined by disc diffusion assay. phenotypic and genotypic det ...201728684959
emergence of ceftazidime-avibactam resistance and restoration of carbapenem susceptibility in klebsiella pneumoniae carbapenemase-producing k pneumoniae: a case report and review of literature.we used meropenem to successfully treat a patient with bacteremia due to ceftazidime-avibactam-resistant, meropenem- susceptible klebsiella pneumoniae that carried mutant blakpc-3. meropenem was bactericidal against ceftazidime-avibactam- resistant k pneumoniae isolates in vitro. nevertheless, the role of carbapenems in treating such infections remains uncertain, because meropenem resistance is selected readily during passage experiments.201728685153
prevalence and drug resistance characteristics of carbapenem-resistant enterobacteriaceae in hangzhou, china.with the abuse of antimicrobial agents in developing countries, increasing number of carbapenem-resistant enterobacteriaceae (cre) attracted considerable public concern. a retrospective study was conducted based on 242 cre strains from a tertiary hospital in hangzhou, china to investigate prevalence and drug resistance characteristics of cre in southeast china. bacterial species were identified. antimicrobial susceptibility was examined by broth microdilution method or epsilometer test. resistan ...201728687975
investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management.201728689800
chitosans inhibit the growth and the adhesion of klebsiella pneumoniae and escherichia coli clinical isolates on urinary catheters.the aim of this study was to determine the antimicrobial activity of different chitosans (cs) against typical colonizing pathogens of the urinary tract and to assess their efficacy against bacterial adhesion and the subsequent biofilm formation on urinary catheters.201728689873
in vitro activity of ceftolozane/tazobactam in combination with other classes of antibacterial agents.combination antibiotic therapy has been used successfully to treat some patients with bacterial infections. however, although certain combinations may result in beneficial synergistic activity, others may produce antagonistic effects resulting in poor treatment outcomes. ceftolozane/tazobactam is an antibacterial agent with potent activity against pseudomonas aeruginosa and many other gram-negative pathogens, including extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae. this study ...201728689923
a case of rhegmatogenous retinal detachment at late stage following endogenous bacterial report a case of rhegmatogenous retinal detachment in the late stage, despite the fact that it had previously been halted after intravitreal injection of an antimicrobial agent against endogenous bacterial endophthalmitis (ebe).201728690534
successful liver transplantation despite donor-transmitted esbl-producing klebsiella pneumoniae infection: case report and review of the literature.donor-derived bacterial infection is a recognized complication of solid organ transplantation. patients admitted to the intensive care unit are increasingly exposed to infection with multidrug-resistant microorganisms. however, no specific recommendations are available about their suitability as donors. we report a case of donor-transmitted extended-spectrum beta-lactamase-producing klebsiella pneumoniae infection in a liver recipient, and review the related literature.201728691772
antibiofilm potential of 16-oxo-cleroda-3, 13(14) e-diene-15 oic acid and its five new γ-amino γ-lactone derivatives against methicillin resistant staphylococcus aureus and streptococcus mutans.the clerodane diterpenoids 16-oxo-cleroda-3, 13(14) e-diene-15 oic acid (1) and kolavenic acid (2) isolated from polyalthia longifolia var. pendula (linn.) were previously reported for their antimicrobial activity. thus present study was designed to investigate the biofilm inhibiting potential of these diterpenoids (1-2) and five new lactone derivatives (3-7) of 1 against methicillin resistant staphylococcus aureus (mrsa), streptococcus mutans, klebsiella pneumoniae and proteus mirabilis. compou ...201728692914
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