effects of scutellaria baicalensis on activity and biofilm formation of klebsiella pneumoniae.objective to explore the effects of scutellaria baicalensis on activity and biofilm formation of klebsiella pneumonia (kp).methods the broth and agar dilution methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of scutellaria baicalensis for tw518. vitek-32 system was used to assay tw518 susceptibility to antibiotics. kp biofilms were formed in vitro and stained with baclight live/dead stain. the class integron geneⅰ1 mrna expression was ...201628803607
isobutanol and 2-ketoisovalerate production by klebsiella pneumoniae via a native pathway.isobutanol is a valuable chemical and is considered a new generation biofuel. construction of isobutanol synthesis pathways in bacteria is a hot topic in isobutanol production. here, we show that an isobutanol synthesis pathway exists naturally in klebsiella pneumoniae; however, this pathway is dormant in the wild-type bacterium. k. pneumoniae is a 2,3-butanediol producer, and the synthesis pathways of 2,3-butanediol, valine and isobutanol all start from condensation of two pyruvate molecules to ...201728802880
discovery of novel highly active and stable aspartate dehydrogenases.aspartate family amino acids (afaas) have important commercial values due to their wide spectrum of applications. most if not all afaas are produced under aerobic conditions which is energy-intensive. to establish a cost-effective anaerobic process for production of afaas, it holds great promise to develop a new pathway enabling the conversion of oxoloacetate into aspartate through direct amination which is catalyzed by aspartate dehydrogenase (aspdh). compared with the well studied aspartate am ...201728801651
prostatic abscess after stapled hemorrhoidopexy caused by esbl extended spectrum beta lactamase producing klebsiella pneumoniae: an additional challenge to postoperative sepsis.postoperative septic complications of hemorrhoids surgical interventions are rare, but very serious with high mortality rate. early diagnosis and prompt therapy are essential to save patient's life. there are a good number of articles and case reports about these septic complications. we are presenting a case report of a prostatic abscess caused by extended spectrum beta lactamase (esbl) producing klebsiella pneumoniae after hemorrhoidopexy. our patient was a healthy middle aged saudi male who h ...201728798879
impact of carbapenem-resistant klebsiella pneumoniae (cr-kp) infections in kidney transplantation.carbapenem-resistant klebsiella pneumoniae (cr-kp) infections in solid organ transplant patients are progressively increasing and are associated with worse outcomes, although potential risk factors and therapeutic strategies are still not well defined.201728796391
successful containment of the first kpc-producing klebsiella pneumoniae outbreak in croatia.we report the first outbreak caused by klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae sequence type 258 (st 258) clone that occurred in croatia from may to december 2012.201728795847
structural and functional insights into the lipopolysaccharide abc transporter lptb2fg.the cell surface of most gram-negative bacteria contains lipopolysaccharide that is essential for their viability and drug resistance. a 134-kda protein complex lptb2fg is unique among atp-binding cassette transporters because it extracts lipopolysaccharide from the external leaflet of the inner membrane and propels it along a filament that extends across the periplasm to directly deliver lipopolysaccharide into the external leaflet of the outer membrane. here we report the crystal structure of ...201728790314
molecular epidemiology of klebsiella pneumoniae invasive infections over a decade at kilifi county hospital in kenya.multidrug resistant (mdr) klebsiella pneumoniae is a common cause of nosocomial infections worldwide. recent years have seen an explosion of resistance to extended-spectrum β-lactamases (esbls) and emergence of carbapenem resistance. here, we examine 198 invasive k. pneumoniae isolates collected from over a decade in kilifi county hospital (kch) in kenya. we observe a significant increase in mdr k. pneumoniae isolates, particularly to third generation cephalosporins conferred by esbls. using who ...201728789913
diversity, virulence, and antimicrobial resistance of the kpc-producing klebsiella pneumoniae st307 clone.the global spread of klebsiella pneumoniae producing klebsiella pneumoniae carbapenemase (kpc) has been mainly associated with the dissemination of high-risk clones. in the last decade, hospital outbreaks involving kpc-producing k. pneumoniae have been predominantly attributed to isolates belonging to clonal group (cg) 258. however, results of recent epidemiological analysis indicate that kpc-producing sequence type (st) 307, is emerging in different parts of the world and is a candidate to beco ...201728785421
β-lacta(®) test performance for detection of extended-spectrum-β-lactamase-producing gram negative bacilli directly on bronchial aspirates samples: a validation study.incidence of extended spectrum β-lactamase-producing gram negative bacilli (esbl-p-gnb)-related infections is worryingly increasing worldwide. esbl-p-gnb detection directly on bronchial aspirate samples (bas) performed for suspected pneumonia may help save empirical carbapenems. our objectives were to optimize β-lacta(®) test (blt) realization and evaluate blt performance for esbl-p-gnb detection directly on bas.201728782646
emergence of low virulent carbapenem-resistant hypermucoviscous klebsiella pneumoniae in china. 201728782563
whole-genome sequencing of human clinical klebsiella pneumoniae isolates reveals misidentification and misunderstandings of klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae.klebsiella pneumoniae is a major threat to public health, causing significant morbidity and mortality worldwide. the emergence of highly drug-resistant strains is particularly concerning. there has been a recognition and division of klebsiella pneumoniae into three distinct phylogenetic groups: klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae. k. variicola and k. quasipneumoniae have often been described as opportunistic pathogens that have less virulence in humans tha ...201728776045
novel durable antimicrobial ceramic with embedded copper sub-microparticles for a steady-state release of copper ions.using pottery clay, porous ceramic stones were molded and then decorated with copper sub-microparticles inside the pores. copper added antimicrobial functionality to the clay-based ceramic and showed ability in disinfecting water. populations of both staphylococcus aureus and klebsiella pneumoniae in contaminated water were reduced by >99.9% in 3 h when exposed to an antimicrobial stone. this antimicrobial performance is attributed to a slow release of copper into water at both room and elevated ...201728773135
automated patterning and probing with multiple nanoscale tools for single-cell analysis.the nano-manipulation approach that combines focused ion beam (fib) milling and various imaging and probing techniques enables researchers to investigate the cellular structures in three dimensions. such fusion approach, however, requires extensive effort on locating and examining randomly-distributed targets due to limited field of view (fov) when high magnification is desired. in the present study, we present the development that automates 'pattern and probe' particularly for single-cell analy ...201728772204
successful ceftazidime-avibactam treatment of mdr-kpc-positive klebsiella pneumoniae infection in a patient with traumatic brain injury: a case report.carbapenem-resistant enterobacteriaceae infections are a serious health care problem, because of the high mortality. carbapenem resistance is mainly caused by carbapenemases production, including klebsiella pneumoniae carbapenemase (kpc). ceftazidime-avibactam is a new cephalosporin/β-lactamase inhibitor combination for the treatment of complicated urinary, intra-abdominal infections, and nosocomial pneumonia caused by gram negative, or other serious gram-negative infections.201728767588
population pharmacokinetics of high dose continuous infusion meropenem and considerations for the use in the treatment of infections due to kpc-producing klebsiella pneumoniae.we assessed the population pharmacokinetics of high-dose continuous infusion (hdci) meropenem in a cohort of patients with kpc-producing klebsiella pneumoniae (kpc-kp) infections. monte carlo simulations were used to define the permissible hdci meropenem regimens that could be safely considered for the treatment of kpc-kp infections due to meropenem-resistant strains. permissible doses were arbitrarily defined as those associated with ≤10-15% likelihood of meropenem steady-state concentrations ( ...201728760900
comprehensive arrayed transposon mutant library of klebsiella pneumoniae outbreak strain kpnih1.klebsiella pneumoniae and other carbapenem-resistant enterobacteriaceae are a major cause of hospital-acquired infections, yet the basis of their success as nosocomial pathogens is poorly understood. to help provide a foundation for genetic analysis of k. pneumoniae, we created an arrayed, sequence-defined transposon mutant library of an isolate from the 2011 outbreak of infections at the u.s. national institutes of health clinical center. the library is made up of 12,000 individually arrayed mu ...201728760848
active sites of o2-evolving chlorite dismutases probed by halides and hydroxides and new iron-ligand vibrational correlations.o2-evolving chlorite dismutases (clds) fall into two subfamilies, which efficiently convert clo2(-) to o2 and cl(-). the cld from dechloromonas aromatica (dacld) represents the chlorite-decomposing homopentameric enzymes found in perchlorate- and chlorate-respiring bacteria. the cld from the gram-negative human pathogen klebsiella pneumoniae (kpcld) is representative of the second subfamily, comprising homodimeric enzymes having truncated n-termini. here steric and nonbonding properties of the d ...201728758386
antioxidant and hepatoprotective potentials of novel endophytic fungus achaetomium sp., from euphorbia isolate, identify and evaluate the antioxidant, antimicrobial, hepatoprotective potentials, total phenolic content, flavonoid content, tannin content of ethyl acetate extract of endophytic fungus achaetomium sp., isolated from euphorbia hirta.201728756924
retro analog concept: comparative study on physico-chemical and biological properties of selected antimicrobial peptides.increasing drug resistance of common pathogens urgently needs discovery of new effective molecules. antimicrobial peptides are believed to be one of the possible solutions of this problem. one of the approaches for improvement of biological properties is reversion of the sequence (retro analog concept). this research is based on investigation of antimicrobial activity against gram-positive, gram-negative bacteria, and fungi, hemolysis of erythrocytes, interpretation of the circular dichroism spe ...201728756544
antibiofilm potential of synthetic 2-amino-5-chlorobenzophenone schiff bases and its confirmation through fluorescence microscopy.antibacterial/antibiofilm potential of microwave-assisted synthetic thirty-three 2-amino-5-chloro benzophenone schiff bases have been carried out against four bacterial strains i.e. klebsiella pneumoniae, proteus mirabilis, staphylococcus aureus and streptococcus mutans. among them compounds 5, 6, 8, 9, 14, 16, 22, 24, 26, and 30-32 showed antibiofilm activities against isolates at less than 100 μg/ml concentrations. these compounds showed enhanced antibiofilm activity against s. aureus as compa ...201728755874
in-vivo loss of carbapenem resistance by extensively drug-resistant klebsiella pneumoniae during treatment via porin expression modification.klebsiella pneumoniae, an enterobacteriaceae that mostly causes hospital-acquired infections, belongs to the recently published who's list of antibiotic-resistant pathogens that pose the greatest threat to human health. indeed, k. pneumoniae is the enterobacterial species most concerned by both resistance to extended-spectrum cephalosporins, due to extended-spectrum β-lactamase (esbl) production, and resistance to carbapenems, i.e. the β-lactams with the broadest activity. carbapenem resistance ...201728751669
risk factors for carbapenem-resistant klebsiella pneumoniae infection: a meta-analysis.carbapenem-resistant klebsiella pneumoniae (crkp) infection has been rapidly emerging as a life-threatening nosocomial disease in many countries. however, studies on the corresponding risk factors of crkp infection showed inconsistent results. to resolve these inconsistencies, we conducted a meta-analysis of previous studies on the potential risk factors of crkp infection. the results of this study could be used to develop crkp infection prevention strategies.201728749714
klebsiella pneumoniae isolates from meningitis: epidemiology, virulence and antibiotic resistance.klebsiella pneumoniae (kp) resistance to broad-spectrum cephalosporin (bsc) in meningitis is important because of limited therapeutic options. to investigate the antibiotic resistance, virulence and epidemiology of kp in meningitis, we conducted a retrospective study for 33 non-metastatic isolates, including primary meningitis (n = 20) and post-craniotomy meningitis (n = 13) collected from 1999 to 2013. bsc resistance was found in 9 (27.3%) isolates, all from post-craniotomy meningitis, harborin ...201728747788
discovery of monoclonal antibodies cross-reactive to novel subserotypes of k. pneumoniae o3.klebsiella pneumoniae is responsible for nosocomial infections causing significant morbidity and mortality. treatment of newly emerging multi-drug resistant strains is hampered due to severely limited antibiotic choices. passive immunization targeting lps o-antigens has been proposed as an alternative therapeutic option, given the limited variability of klebsiella o-antigens. here we report that the o3 serogroup, previously considered to have uniform o-antigen built of mannan, represents three d ...201728747785
instability of the incfii-type plasmid carrying blandm-5 in a klebsiella pneumoniae this study, we characterized the blandm-5-bearing plasmid in a klebsiella pneumoniae isolate that had lost the plasmid during serial passage. we determined the complete sequences of the plasmid pcc1410-2, which was extracted from a k. pneumoniae st709 isolate collected at a korean hospital from which two ndm-5-producing k. pneumoniae isolates were subsequently isolated. as a result, the pcc1410-2 plasmid had a backbone structure that was similar to those of two plasmids previously reported fr ...201728746989
molecular epidemiology of carbapenem-resistant klebsiella pneumonia at a turkish center: is the increase of resistance a threat for europe?in recent years carbapenem resistant klebsiella pneumoniae has became an important threat to hospitalized patients. our aim in this study was to identify the genetic mechanisms of carbapenem resistance in our center.201728743652
retention of virulence following colistin adaptation in klebsiella pneumoniae is strain-dependent rather than associated with specific mutations.this study aimed to understand the impact on virulence and fitness of mutations in specific genes found after adaptation of klebsiella pneumoniae to colistin. isolates with an increase in their inhibitory concentration (mic) to colistin of 32- to >128-fold were shown to have mutations in mgrb, phopq and pmrab, all known regulators of pathways affecting membrane lipid content. when these strains were used in studies in galleria mellonella there was no clear correlation between mutations in specif ...201728741998
resistance to ceftazidime-avibactam is due to transposition of kpc in a porin-deficient strain of klebsiella pneumoniae with increased efflux activity.ceftazidime-avibactam is an antibiotic with activity against serine beta-lactamases, including kpc. recently, reports have emerged of kpc-producing isolates resistant to this antibiotic, including a report of a wild-type kpc-3 producing sequence type 258 klebsiella pneumoniae that was resistant to ceftazidime-avibactam. we describe a detailed analysis of this isolate, in the context of two other closely related kpc-3 producing isolates, recovered from the same patient. both isolates encoded a no ...201728739787
colistin resistance in kpc-2- and shv-5-producing klebsiella pneumoniae clinical isolates in bulgaria.colistin resistance is increasingly recognized among carbapenemase-producing klebsiella pneumoniae isolates in several european regions. the current study documents the appearance of colistin resistance among kpc-2 and shv-5-produning k. pneumoniae strains in bulgaria.201728738348
prevalence of a new variant oxa-204 and oxa-48 carbapenemases plasmids encoded in klebsiella pneumoniae clinical isolates in tunisia.carbapenemase-producing klebsiella pneumoniae strains have emerged as a major problem for healthcare systems. the aim of this study was to determine the role and diversity of plasmids harboring carbapenemases encoding genes from a collection of k. pneumoniae isolates recovered between july 2011 and january 2012, with decreased susceptibility to carbapenems. imipenem (ipm), ertapenem (etp), meropenem (mem), and doripenem (dor) minimum inhibitory concentrations (mics) were determined by e-test. ca ...201728737463
hypermucoviscosity, rmpa, and aerobactin are associated with community-acquired klebsiella pneumoniae bacteremic isolates causing liver abscess in singapore.this retrospective study investigated the clinical etiology of community-acquired bacteremic klebsiellapneumoniae infections, and characterized laboratory and genetic markers which may be associated with primary liver abscess (pla).201728736222
trends of bloodstream infections in a university greek hospital during a three-year period: incidence of multidrug-resistant bacteria and seasonality in gram-negative predominance.the aim of the study was to assess the epidemiology, the incidence of multidrug-resistant bacteria and bloodstream infections' (bsis) seasonality in a university hospital. this retrospective study was carried out in the university general hospital of patras, greece, during 2011-13 y. blood cultures from patients with clinical presentation suggestive of bloodstream infection were performed by the bact/alert system. isolates were identified by vitek 2 advanced expert system. antibiotic susceptibil ...201728735318
early kpc-producing klebsiella pneumoniae bacteremia among intensive care unit patients non-colonized upon admission.among 140 patients colonized by kpc-producing klebsiella pneumoniae (kpc-kp) between fourth and seventh day of intensive care unit stay, 24 developed bacteraemia immediately after colonization. colistin-resistance of the colonizing isolate was the factor significantly associated with early kpc-kp bacteraemia (p < 0.001; or 6.6, 95% ci 2.4-18.4), a worrisome finding since infections by colistin-resistant isolates is associated with increased mortality due to limited remaining therapeutic options.201728735313
antimicrobial resistance profiles and genotypes of extended-spectrum β-lactamase- and ampc β-lactamase-producing klebsiella pneumoniae isolated from dogs in beijing, china.klebsiella pneumoniae, which exists in the intestinal and respiratory tracts of humans and animals, is an important conditional pathogen in many animals. the aim of the current study was to investigate the antimicrobial resistance profiles and genotypes of extended-spectrum β-lactamase (esbl)- and ampc β-lactamase-producing k. pneumoniae isolated from dogs.201728735050
occurrence and clonal diversity of multidrug-resistant klebsiella pneumoniae recovered from inanimate surfaces in algerian hospital environment: first report of arma, qnrb and aac(6')-ib-cr genes.the aim of this study is to characterize the molecular support of antibiotic resistance in mdr klebsiella pneumoniae recovered from inanimate surfaces between march 2012 to february 2014 in three teaching hospitals (setif, bejaia and constantine) in algeria.201728732788
the kp1_4563 gene is regulated by the camp receptor protein and controls type 3 fimbrial function in klebsiella pneumoniae ntuh-k2044.klebsiella pneumoniae (k. pneumoniae) is an opportunistic pathogen that can adhere to host cells or extracellular matrix via type 1 and type 3 fimbriae. kp1_4563 is a gene encoding a hypothetical protein in k. pneumoniae ntuh-k2044. kp1_4563 is located between the type 1 and type 3 fimbrial gene clusters and is likely associated with fimbrial function given its putative conserved domains of unknown function (duf1471). cyclic amp receptor protein (crp) regulates virulence-related gene expression ...201728732013
biofilm formation and antibiotic resistance in klebsiella pneumoniae urinary strains.multidrug-resistant klebsiella pneumoniae has become a relevant healthcare-associated pathogen. capsule, type 1 and 3 fimbriae (mrka gene), type 2 quorum-sensing system (luxs), synthesis of d-galactan i (wbbm), lps transport (wzm) and poly-beta-1,6-n-acetyl-d-glucosamine (pgaa) seem involved in k. pneumoniae biofilm. nonenzymatic antibiotic resistance is related to nonexpression or mutation of porins (ompk35 and ompk36), and efflux pump (acrb) overexpression. the aim of this study was to analyse ...201728731269
relating switching rates between normal and persister cells to substrate and antibiotic concentrations: a mathematical modelling approach supported by experiments.we developed and compared two mathematical models of variable phenotypic switching rates between normal and persister cells that depend on substrate concentration and antibiotic presence. they could be used to simulate the formation of persisters in environments with concentration gradients such as biofilms. our models are extensions of a previous model of the dynamics of normal and persistent cell populations developed by balaban et al. (2004, science 305: 1622). we calibrated the models' param ...201728730700
first two cases of severe multifocal infections caused by klebsiella pneumoniae in switzerland: characterization of an atypical non-k1/k2-serotype strain causing liver abscess and endocarditis.we describe the first two multifocal invasive infections due to klebsiella pneumoniae recently observed in switzerland.201728729207
spontaneous globe rupture due to rapidly evolving endogenous hypermucoid klebsiella pneumoniae this retrospective report, the authors describe a rare case of spontaneous globe rupture from klebsiella pneumoniae endophthalmitis in a middle-aged man with poorly controlled type 2 diabetes mellitus. there have been only four previously reported cases of spontaneous globe rupture from endophthalmitis. out of the now five reported cases, all have been due to endogenous endophthalmitis, four have been due to gram-negative bacteria, and three have been due to k. pneumoniae. k. pneumoniae, espe ...201728728189
genomic characterization of novel incfii-type multidrug resistant plasmids p0716-kpc and p12181-kpc from klebsiella pneumoniae.this study aimed to genetically characterize two fully-sequenced novel incfii-type multidrug resistant (mdr) plasmids, p0716-kpc and p12181-kpc, recovered from two different clinical klebsiella pneumoniae isolates. p0716-kpc and p12181-kpc had a very similar genomic content. the backbones of p0716-kpc/p12181-kpc contained two different replicons (belonging to a novel incfii subtype and the rep_3 family), the incfiik and incfiiy maintenance regions, and conjugal transfer gene sets from incfiik-ty ...201728725038
long-term care facility (ltcf) residents colonized with multidrug-resistant (mdr) klebsiella pneumoniae lineages frequently causing infections in portuguese clinical institutions. 201728724454
identification of antipneumococcal molecules effective against different streptococcus pneumoniae serotypes using a resazurin-based high-throughput screen.streptococcus pneumoniae is a major human pathogen, causing around 1.6 million deaths worldwide each year. by optimizing a resazurin-based assay to detect s. pneumoniae growth in 384-well microplates, we developed a new high-throughput screening (hts) system for the discovery of antipneumococcal molecules, which was unsuccessful using conventional absorbance measurements. before applying our protocol to a large-scale screen, we validated the system through a pilot screen targeting about 7,800 bi ...201728723269
reaction-diffusion theory explains hypoxia and heterogeneous growth within microbial biofilms associated with chronic infections.reaction-diffusion models were applied to gain insight into the aspects of biofilm infection and persistence by comparing mathematical simulations with the experimental data from varied bacterial biofilms. these comparisons, including three in vitro systems and two clinical investigations of specimens examined ex vivo, underscored the central importance of concentration gradients of metabolic substrates and the resulting physiological heterogeneity of the microorganisms. relatively simple one-di ...201628721248
new delhi metallo-beta-lactamase (ndm-1)-producing klebsiella pneumoniae isolated from a burned patient.background infections affecting burn patients are frequently caused by staphylococcus aureus, pseudomonas aeruginosa, and enterobacteriaceae species. infections with these pathogens have become increasingly difficult to treat due to evolving antibiotic resistance mechanisms, including the production of carbapenemases. case report the present case report describes the evolution of a burn patient with polymicrobial healthcare-associated burn infections, including a bloodstream infection due to an ...201728717120
the metabolite transporter peg344 is required for the full virulence of the hypervirulent klebsiella pneumoniae strain hvkp1 after pulmonary, but not subcutaneous challenge.hypervirulent klebsiella pneumoniae (hvkp) is an emerging pathotype that is capable of causing tissue-invasive, organ and life threatening infections in healthy individuals from the community. knowledge on the virulence factors specific to hvkp is limited. in this report we describe a new factor (peg344) that increases the virulence of the hvkp strain hvkp1. peg344 is present on the hvkp1 virulence plasmid, is broadly prevalent among hvkp strains, and has increased rna abundance when grown in hu ...201728717029
phosphoenolpyruvate phosphotransferase system components positively regulate klebsiella biofilm formation.klebsiella pneumoniae is one of the leading causes of device-related infections (dris), which are associated with attachment of bacteria to these devices to form a biofilm. the latter is composed of not only bacteria but also extracellular polymeric substances (epses) consisting of extracellular dnas, polysaccharides, and other macromolecules. the phosphoenolpyruvate (pep):carbohydrate phosphotransferase system (pts) regulates diverse processes of bacterial physiology. in the genome of k. pneumo ...201728716362
comparison of synergism between colistin, fosfomycin and tigecycline against extended-spectrum β-lactamase-producing klebsiella pneumoniae isolates or with carbapenem investigate the synergistic and bactericidal effects of antimicrobial combinations of any two of colistin, fosfomycin and tigecycline against the nine extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae (kp) clinical isolates, including 4 carbapenem-susceptible strains and five imipenem and/or meropenem-resistant strains.201728716360
production of chemicals by klebsiella pneumoniae using bamboo hydrolysate as feedstock.bamboo is an important biomass, and bamboo hydrolysate is used by klebsiella pneumoniae as a feedstock for chemical production. here, bamboo powder was pretreated with naoh and washed to a neutral ph. cellulase was added to the pretreated bamboo powder to generate the hydrolysate, which contained 30 g/l glucose and 15 g/l xylose and was used as the carbon source to prepare a medium for chemical production. when cultured in microaerobic conditions, 12.7 g/l 2,3-butanediol was produced by wildtype ...201728715380
clinical outcomes associated with carbapenem resistant klebsiella pneumoniae (crkp) in abdominal solid organ transplant (sot) recipients. 201728714754
synthetic multispecies microbial communities reveals shifts in secondary metabolism and facilitates cryptic natural product discovery.chemically mediated interactions have been hypothesized to be essential for ecosystem functioning as co-occurring organisms can influence the performance of each other by metabolic means. here, we present a co-culture device that allows co-culturing of microorganisms that are physically separated but can exchange chemical signals and metabolites. this setup was adopted to perform investigations on the secondary metabolisms of both a fungal-bacterial community and an actinomycetic-actinomycetic c ...201728714207
extensively drug-resistant klebsiella pneumoniae causing nosocomial bloodstream infections in china: molecular investigation of antibiotic resistance determinants, informing therapy, and clinical outcomes.the rise in diversity of antimicrobial resistance phenotypes seen in klebsiella pneumoniae is becoming a serious antibiotic management problem. we sought to investigate the molecular characteristics and clinical implications of extensively drug-resistant (xdr) k. pneumoniae isolated from different nosocomial bloodstream infections (bsis) patients from july 2013 to november 2015. even in combination treatment, meropenem did not protect against mortality of bsis patients (p = 0.015). in contrast, ...201728713357
a hospital-based matched case-control study to identify risk factors for clinical infection with oxa-48-producing klebsiella pneumoniae in rectal carriers.asymptomatic colonisation of the gastrointestinal tract by carbapenemase-producing enterobacteriaceae is an important reservoir for transmission, which may precede infection. this retrospective observational case-control study was designed to identify risk factors for developing clinical infection with oxa-48-producing klebsiella pneumoniae in rectal carriers during hospitalisation. case patients (n = 76) had carbapenemase-producing k. pneumoniae (cpkp) infection and positive rectal culture for ...201728712369
phenotypic and genotypic characterization of clinically relevant bacteria isolated from dental waste and waste workers' hands, mucosas and coats.infectious wastes are potential sources of pathogenic micro-organisms, which may represent a risk to the professionals who manage them. in this study, we aimed to characterize the infectious bacteria present in dental waste and waste workers. the dental waste produced over 24 h was collected and waste workers were sampled by swabbing. isolate resistance profiles were characterized by vitek(®) and pcr and biofilm formation by congo red agar, string test and microtitre assay. to assess similarity ...201728712134
outbreak of colistin-resistant carbapenemase-producing klebsiella pneumoniae in tunisia.mechanisms of colistin and carbapenem resistance among a collection of klebsiella pneumoniae isolates recovered in a university hospital in tunisia were studied.201728711559
effectiveness of a double-carbapenem regimen in a kpc-producing klebsiella pneumoniae infection in an immunocompromised patient.the progressive increase of infections produced by extensively drug-resistant carbapenemase-producing klebsiella pneumoniae (xdr-cpkp) represents an important threat to public health. unfortunately, optimal therapeutic options are scarce. retrospective studies have recommended combined therapy with more than one antibiotic and, more recently, a double-carbapenem regimen has been reported to be an effective alternative therapy. here, we describe an episode of sepsis in an immunocompromised patien ...201728708458
detection of extended-spectrum beta-lactamases (esbls) and antibiotic susceptibility patterns in klebsiella pneumoniae in western, iran.production of beta-lactamase enzymes, especially extended-spectrum beta -lactamases (esbl), is a primary mechanism of resistance in these bacteria. the purpose of this study was detection of blatem, blashv, blactx-m, blactx-m-15, blaper and blaveb in k. pneumoniae, isolated from clinical specimens by the pcr method and antibiotic susceptibility patterns in these strains isolated, in sanandaj city, iran.201728707597
draft whole-genome assemblies of drug-resistant clinical isolates of klebsiella pneumoniae from the, we report the draft assemblies of 11 clinical isolates of klebsiella pneumoniae that are resistant to cephalosporins, carbapenems, and/or colistin. the assemblies ranged from 5.37 mbp to 5.70 mbp in size. several plasmid sequences were present, and resistance genes spanning multiple classes of antibiotics were predicted.201728705960
the plasmid-mediated fosfomycin resistance determinants and synergy of fosfomycin and meropenem in carbapenem-resistant klebsiella pneumoniae isolates in taiwan.epidemiology of fosfomycin susceptibility and the plasmid-mediated fosfomycinase genes of carbapenem-resistant klebsiella pneumoniae (crkp) isolates in taiwan remain unclear.201728705769
molecular detection of β-lactamase and integron genes in clinical strains of klebsiella pneumoniae by multiplex polymerase chain reaction.infections caused by β-lactamase-producing gram-negative bacteria, such as klebsiella pneumoniae, are increasing globally with high morbidity and mortality. the aim of the current study was to determine antimicrobial susceptibility patterns and the prevalence of antibiotic resistance genes (β-lactamase and integron genes) using multiplex pcr.201728700049
st37 klebsiella pneumoniae: development of carbapenem resistance in vivo during antimicrobial therapy in investigate the mechanism leading to in vivo carbapenem resistance development in klebsiella pneumoniae.201728699768
prevalence and etiology of bacteremia in febrile children with sickle cell disease at a nigeria tertiary a result of immune defects in sickle cell disease (scd), affected individuals are prone to infection from encapsulated bacterial pathogens like streptococcus pneumoniae. studies on the etiological agents of bacteremia in children with scd in nigeria are few and have revealed a spectrum of organisms that is different from those recorded in other parts of the world.201728698782
double carbapenem therapy (dct) for bacteremia due to carbapenem-resistant klebsiella pneumoniae (crkp): from test tube to clinical practice. 201728695756
antimicrobial natural product research: a review from a south african perspective for the years 2009-2016.this review provides information on the antimicrobial research which has taken place on south african natural products for the last eight years (2009-2016). this important field is the backbone of all studies involving the use of medicinal plants against infectious diseases and hence can form the mainstay for future studies.201728694104
the prevalence of rectal carriage of klebsiella pneumoniae amongst diabetic patients and their clinical relevance in taiwan: a five-year prospective study.pyogenic liver abscess (pla) and bacteremia caused by klebsiella pneumoniae is a common complication among patients with diabetes mellitus (dm). the aim of this study is to investigate the prevalence of rectal carriage and serotype distribution of k. pneumoniae amongst dm patients and their clinical relevance.201728693930
prevalence of carbapenem-resistant klebsiella pneumoniae (crkp) and the distribution of class 1 integron in their strains isolated from a hospital in central china.objective the aim of our study is to investigate the prevalence of carbapenem-resistant klebsiella pneumoniae (crkp) and the genetic characteristics of the class 1 integron in crkp on multi-drug resistance.methods clinical klebsiella pneumoniae strains were collected from multiple departments of a hospital in central china. crkp strains were identified among the isolates, and antibiotics susceptibility of crkp strains was analyzed. the polymerase chain reaction (pcr) was adopted to amplify the c ...201728693691
resolution of carbapenemase-producing klebsiella pneumoniae outbreak in a tertiary cancer center; the role of active surveillance. 201728693656
antibiofilm potential of 16-oxo-cleroda-3, 13(14) e-diene-15 oic acid and its five new γ-amino γ-lactone derivatives against methicillin resistant staphylococcus aureus and streptococcus mutans.the clerodane diterpenoids 16-oxo-cleroda-3, 13(14) e-diene-15 oic acid (1) and kolavenic acid (2) isolated from polyalthia longifolia var. pendula (linn.) were previously reported for their antimicrobial activity. thus present study was designed to investigate the biofilm inhibiting potential of these diterpenoids (1-2) and five new lactone derivatives (3-7) of 1 against methicillin resistant staphylococcus aureus (mrsa), streptococcus mutans, klebsiella pneumoniae and proteus mirabilis. compou ...201728692914
successful liver transplantation despite donor-transmitted esbl-producing klebsiella pneumoniae infection: case report and review of the literature.donor-derived bacterial infection is a recognized complication of solid organ transplantation. patients admitted to the intensive care unit are increasingly exposed to infection with multidrug-resistant microorganisms. however, no specific recommendations are available about their suitability as donors. we report a case of donor-transmitted extended-spectrum beta-lactamase-producing klebsiella pneumoniae infection in a liver recipient, and review the related literature.201728691772
a case of rhegmatogenous retinal detachment at late stage following endogenous bacterial report a case of rhegmatogenous retinal detachment in the late stage, despite the fact that it had previously been halted after intravitreal injection of an antimicrobial agent against endogenous bacterial endophthalmitis (ebe).201728690534
emergence of ceftazidime-avibactam resistance and restoration of carbapenem susceptibility in klebsiella pneumoniae carbapenemase-producing k pneumoniae: a case report and review of literature.we used meropenem to successfully treat a patient with bacteremia due to ceftazidime-avibactam-resistant, meropenem- susceptible klebsiella pneumoniae that carried mutant blakpc-3. meropenem was bactericidal against ceftazidime-avibactam- resistant k pneumoniae isolates in vitro. nevertheless, the role of carbapenems in treating such infections remains uncertain, because meropenem resistance is selected readily during passage experiments.201728685153
association between virulence factors and extended spectrum beta-lactamase producing klebsiella pneumoniae compared to nonproducing isolates.klebsiella pneumoniae is considered an important opportunistic multidrug-resistant pathogen. extended spectrum β-lactamases (esbls) and expression of a multitude of virulence factors may work in a harmony resulting in treatment failure. this study was undertaken to compare the virulence characteristics and genetic relatedness between esbl and non-esbl producing k. pneumoniae. methods. antibiotic sensitivity test of all isolates was determined by disc diffusion assay. phenotypic and genotypic det ...201728684959
draft genome sequence of a multidrug-resistant sequence type 231 outbreak-associated clone of klebsiella pneumoniae, kp41-2015, producing oxa-232 carbapenemase.carbapenem-resistant klebsiella pneumoniae infection is a rising public health threat due to limited therapeutic options. here, we report the genome sequence of a multidrug-resistant k. pneumoniae sequence type 231 (st231) strain associated with an outbreak of infections in an intensive care unit that carries a unique complement of resistance determinants.201728684582
complete genome sequence of a multidrug-resistant, blandm-1-expressing klebsiella pneumoniae k66-45 clinical isolate from norway.multidrug-resistant klebsiella pneumoniae is a major cause of hospital-acquired infections. here, we report the complete genome sequence of the multidrug-resistant, blandm-1-positive strain k. pneumoniae k66-45, isolated from a hospitalized norwegian patient.201728684580
pneumonia in the developing world: characteristic features and approach to pneumonia (cap) is a common cause of morbidity and mortality in adults worldwide, but its epidemiology varies markedly by region. whilst in high-income countries, the predominant burden of cap is in the elderly and those with chronic cardiovascular and pulmonary co-morbidity, cap patients in low-income settings are often of working age and, in sub-saharan africa, frequently hiv-positive. although region-specific aetiological data are limited, they are sufficient to highlight m ...201728681972
double carbapenem as a rescue strategy for the treatment of severe carbapenemase-producing klebsiella pneumoniae infections: a two-center, matched case-control study.recent reports have suggested the efficacy of a double carbapenem (dc) combination, including ertapenem, for the treatment of carbapenem-resistant klebsiella pneumoniae (cr-kp) infections. we aimed to evaluate the clinical impact of such a regimen in critically ill patients.201728679413
effect of the meropenem mic on the killing activity of meropenem and polymyxin b in combination against kpc-producing klebsiella pneumoniae. 201728676713
dissemination of carbapenem-resistant klebsiella pneumoniae clinical isolates with various combinations of carbapenemases (kpc-2, ndm-1, ndm-4, and oxa-48) and 16s rrna methylases (rmtb and rmtc) in vietnam.twenty-seven clinical isolates of carbapenem-resistant klebsiella pneumoniae with mics ≥4 mg/l for imipenem or meropenem were obtained from inpatients in a hospital in vietnam. antimicrobial susceptibility tests and whole genome sequencing were performed. multilocus sequence typing and the presence of drug resistant genes were determined and a maximum-likelihood phylogenetic tree was constructed by snp alignment of whole genome sequencing data.201728676118
emerging trends of nosocomial pneumonia in intensive care unit of a tertiary care public teaching hospital in western india.nosocomial pneumonia poses great challenge to an intensivist. detailed information about hospital-acquired pneumonia (hap) and ventilator-acquired pneumonia (vap) is crucial for prevention and optimal management, thus improving quality intensive care unit (icu) care. hence, we aimed to study the current trend of nosocomial pneumonia in icu.201728671150
carbapenem resistant klebsiella pneumoniae isolated from bloodstream infection: indian experience.increased incidence of multidrug resistant (mdr) gram negative infection has resulted in high rates of morbidity and mortality. klebsiella pneumoniae is one of the commonest mdr pathogens causing bacteraemia with limited therapeutic options such as colistin and tigecycline. present study focused on molecular characterisation of mdr k. pneumoniae from bloodstream infection and their clinical outcome. a total of 115 k. pneumoniae from january 2015 to september 2016 were included in the study which ...201728670975
in vitro pharmacokinetics/pharmacodynamics evaluation of fosfomycin combined with amikacin or colistin against kpc2-producing klebsiella pneumoniae.objectives: the emergence of carbapenem-resistant enterobacteriaceae, especially klebsiella pneumoniae, has become a major concern in clinic settings. combination therapy is gaining momentum to counter the secondary resistance and potential suboptimal efficacy of monotherapy. the aim of this study was to evaluate the bactericidal effect of fosfomycin (fm), amikacin (amk), or colistin (col) alone and combinations against kpc2-producing k. pneumoniae using dynamic model by simulating human pharmac ...201728670570
molecular characterization of klebsiella pneumoniae isolates from stool specimens of outpatients in sentinel hospitals beijing, china, 2010-2015.klebsiella pneumoniae (k. pneumoniae) is an opportunistic pathogen associated with community-acquired infections and nosocomial infections. from 2010 to 2015, k. pneumoniae testing was included into the exiting diarrhea-syndrome surveillance with objective to estimate the prevalence of k. pneumoniae in diarrhea-syndrome patients, test antibiotics susceptibility and investigate molecular characteristics.201728670346
a novel approach for combating klebsiella pneumoniae biofilm using histidine functionalized silver nanoparticles.treating pathogens is becoming challenging because of multidrug resistance and availability of limited alternative therapies which has further confounded this problem. the situation becomes more alarming when multidrug resistant pathogens form a 3d structure known as biofilm. biofilms are formed in most of the infections especially in chronic infections where it is difficult to eradicate them by conventional antibiotic therapy. chemically synthesized nanoparticles are known to have antibiofilm a ...201728670301
quick 96fasp for high throughput quantitative proteome analysis.filter aided sample preparation (fasp) is becoming a central method for proteomic sample cleanup and peptide generation prior to lc-ms analysis. we previously adapted this method to a 96-well filter plate, and applied to prepare protein digests from cell lysate and body fluid samples in a high throughput quantitative manner. while the 96fasp approach is scalable and can handle multiple samples simultaneously, two key advantages compared to single fasp, it is also time-consuming. the centrifugati ...201728669814
in vivo development of tigecycline resistance in klebsiella pneumoniae owing to deletion of the ramr ribosomal binding site.tigecycline resistance is emerging among klebsiella pneumoniae, but knowledge regarding in vivo development of tigecycline resistance is limited. here we report a new mechanism of tigecycline resistance in k. pneumoniae that evolved during tigecycline therapy. klebsiella pneumoniae isolates were consecutively obtained from urine samples of a patient with scrotal abscess and urinary tract infection before and during tigecycline treatment. two tigecycline-resistant k. pneumoniae strains (kp-3r and ...201728668690
epidemiology, management, and outcome of carbapenem-resistant klebsiella pneumoniae bloodstream infections in hospitals within the same endemic metropolitan the last decade, carbapenem-resistant klebsiella pneumoniae (cr-kp) has become endemic in several countries, including italy. in the present study, we assessed the differences in epidemiology, management, and mortality of cr-kp bloodstream infection (bsi) in the three main adult acute-care hospitals of the metropolitan area of genoa, italy. from january 2013 to december 2014, all patients with cr-kp bsi were identified through the computerized microbiology laboratory databases of the three ho ...201728668656
klebsiella pneumoniae. 201728665914
successful treatment of multidrug resistant klebsiella pneumoniae using dual carbapenem regimen in immunocompromised patient.this report describes a longitudinal case of immunocompromised patient post kidney transplant who was admitted to our institution repeatedly for treatment of various infections caused by multi-drug resistant klebsiella pneumoniae. the patient was successfully treated with a combination of ertapenem/meropenem on multiple occasions despite the elevated mics. our observations corroborate previous preclinical studies and case reports showing the efficacy of double carbapenem regimens against resista ...201728660131
molecular epidemiology and risk factors of carbapenem-resistant klebsiella pneumoniae infections in eastern china.background: the increasing prevalence of carbapenem-resistant klebsiella pneumoniae (crkp) poses an immediate threat to treatment worldwide. this retrospective study assessed the molecular epidemiology and determined the risk factors for and outcomes of crkp infections in a general teaching hospital in shanghai, china. methods: from january 2013 to july 2015, 100 consecutive unique crkp isolates isolated from hospitalized patients were collected. isolates were screened for antibiotic resistance ...201728659886
metastatic endophthalmitis and thyroid abscess complicating klebsiella pneumoniae liver abscess.the thyroid is resistant to infection due to its anatomical and physiological characteristics. we present a rare case of invasive liver abscess with metastatic thyroid abscess and endogenous endophthalmitis in a previously healthy 55-year-old female patient without diabetes or other medical illness. this report raises an important question of the virulence of klebsiella pneumoniae as an increasingly common causative agent of liver abscess.201728656747
stand-alone eal domain proteins form a distinct subclass of eal proteins involved in regulation of cell motility and biofilm formation in enterobacteria.the second messenger cyclic dimeric gmp (c-di-gmp) is almost ubiquitous among bacteria as are the c-di-gmp turnover proteins, which mediate the transition between motility and sessility. eal domain proteins have been characterized as c-di-gmp-specific phosphodiesterases. while most eal domain proteins contain additional, usually n-terminal, domains, there is a distinct family of proteins with stand-alone eal domains, exemplified by salmonella enterica serovar typhimurium proteins stm3611 (yhjh/p ...201728652301
complete dna sequence of an incm1 plasmid bearing the novel qnre1 plasmid-mediated quinolone resistance variant and blactx-m-8 from klebsiella pneumoniae sequence type 147. 201728652231
in vitro antibacterial activity of fosfomycin combined with other antimicrobials against kpc-producing klebsiella pneumoniae.the increasing prevalence of kpc-producing klebsiella pneumoniae (kpc-kp) strains poses a serious threat to patients. therapeutic options are limited to colistin, fosfomycin, tigecycline and selected aminoglycosides. although the combination of fosfomycin with other antimicrobials is recommended, data regarding possible synergistic activity in vitro and in vivo appear inconsistent. here we report that five drug combinations (fosfomycin combined with imipenem, ertapenem, tigecycline, colistin or ...201728648647
higher mortality of severe influenza patients with probable aspergillosis than those with and without other coinfections.aspergillus-associated infection might comprise up to 23-29% of severe influenza patients from the community throughout stay in an intensive care unit (icu). in taiwan, cases of severe influenza with aspergillosis are increasingly reported. therefore, we describe the relative risk of mortality among severe influenza patients with aspergillosis and other coinfections compared to severe influenza patients without aspergillus coinfections.201728647219
multidrug resistance mechanisms of carbapenem resistant klebsiella pneumoniae strains isolated in chongqing, china.carbapenem-resistant klebsiella pneumoniae (crkp) is considered a serious global threat. however, little is known regarding the multidrug resistance (mdr) mechanisms of crkp. this study investigated the phenotypes and mdr mechanisms of crkp and identified their clonal characteristics.201728643488
first description of methyltransferases in extensively drug-resistant klebsiella pneumoniae isolates from saudi arabia.the resistance determinants for carbapenems, fluoroquinolones and aminoglycosides were characterized in 16 extensively drug-resistant klebsiella pneumoniae (xdrkpn) strains collected from saudi hospitals during 2014.201728639934
lipid peroxidation in gram-negative bacteremia modulates the risk for septic shock and infections by resistant klebsiella pneumoniae.controversies in outcomes with the parenteral administration of antioxidants as adjuvant therapies led to the measurement of malondialdehyde (mda), a product of lipid peroxidation, in serum collected from 120 patients with primary gram-negative bacteremia during the first 24 h from sepsis onset. mda was measured by the thiobarbiturate assay, followed by high-performance liquid chromatography (hplc) analysis. after receiver operator characteristic (roc) curve analysis, patients were divided into ...201728639164
molecular identification of aminoglycoside-modifying enzymes and plasmid-mediated quinolone resistance genes among klebsiella pneumoniae clinical isolates recovered from egyptian patients.inappropriate use of antibiotics in clinical settings is thought to have led to the global emergence and spread of multidrug-resistant pathogens. the goal of this study was to investigate the prevalence of genes encoding aminoglycoside resistance and plasmid-mediated quinolone resistance among clinical isolates of klebsiella pneumoniae. all k. pneumoniae isolates were phenotypically identified using api 20e and then confirmed genotypically through amplification of the specific k. pneumoniae phoe ...201728638412
deleting pck improves growth and suppresses by-product formation during 1,3-propanediol fermentation by klebsiella investigate the role of phosphoenolpyruvate (pep) carboxylation in cell metabolism in klebsiella pneumoniae.201728637087
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