relative susceptibility of airway organisms to antimicrobial effects of nitric oxide.nitric oxide (no) is released in the airway as a critical component of innate immune defense against invading pathogenic organisms. it is well documented that bacteriostatic and bactericidal effects of no are concentration-dependent. however, few data exist comparing relative susceptibility of common pathogens to no at physiologic concentrations. in this study we evaluated the effects of no on 4 common airway bacteria and 1 fungus, and examined the potential implications of discrepancies in sens ...201728544570
microbiological analysis of acute infections of the nail fold on the basis of bait thread acute infection of the nail fold, called paronychia, is a common clinical problem. the basis for the implementation of the treatment is the result of microbiological examination. due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced.201728507488
assessment of herbal drugs for promising anti-candida activity.microbial infections are diverse and cause serious human diseases. candida albicans infections are serious healthcare-related infections that are complicated by its morphological switching from yeast to hyphae, resistant biofilm formation and mixed infections with bacteria. due to the increase in drug resistance to currently used antimicrobial agents and the presence of undesirable side effects, the need for safe and effective novel therapies is important. compounds derived from plants are known ...201728482836
synthesis, characterization, antimicrobial and antiproliferative activity evaluation of cu(ii), co(ii), zn(ii), ni(ii) and pt(ii) complexes with isoniazid-derived compound.hydrazone complexes of cu(ii), co(ii), zn(ii), ni(ii) and pt(ii) with n-isonicotinoyl-n'-(3-metoxy-2 hydroxybenzaldehyde)-hydrazone (hl) were synthesized and characterized by different physico-chemical techniques including elemental and thermal analysis, magnetic susceptibility measurements, molar electric conductivity, as well as ir (infrared), ¹h-nmr and (13)c-nmr (hydrogen and carbon nuclear magnetic resonance, uv-vis (ultraviolet-visible), fab (fast atom bombardment), epr (electron paramagne ...201728422067
characterization of oxa-48-like-producing enterobacteriaceae isolated from river water in algeria.the spread of carbapenemase-producing enterobacteriaceae (cpe) is a significant problem for healthcare worldwide. the prevalence of carbapenem-resistant enterobacteriaceae (cpe) in water environments in algeria are unknown. the aim of this study was to screen for the presence of cpe isolates in the soummam river in bejaia, algeria. isolates of enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. the isolate ...201728486169
construction of recombinant pdu metabolosome shells for small molecule production in corynebacterium glutamicum.bacterial microcompartments have significant potential in the area of industrial biotechnology for the production of small molecules, especially involving metabolic pathways with toxic or volatile intermediates. corynebacterium glutamicum is an established industrial workhorse for the production of amino acids and has been investigated for the production of a variety of further value-added products. herein, we describe components for the establishment of bacterial microcompartments as production ...201728826205
high prevalence of extended-spectrum β-lactamase-producing enterobacteriaceae in wild fish from the mediterranean sea in algeria.we investigated the prevalence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae among wild fish from the coast of bejaia (algeria) in the mediterranean sea.201728805537
characterization of the complete nucleotide sequences of inca/c2 plasmids carrying in809-like integrons from enterobacteriaceae isolates of wildlife origin.a total of 18 enterobacteriaceae (17 from gulls and 1 from a clinical sample) collected from australia, carrying inca/c plasmids with the imp-encoding in809-like integrons, were studied. seven plasmids, being representatives of different origins, plasmid sizes, replicon combinations, and resistance genes, were completely sequenced. plasmid pec158, identified in a clinical escherichia coli st752 isolate, showed extensive similarity to type 2 inca/c2 plasmids. pec158 carried none of the blacmy-2-l ...201728696228
wastewater is a reservoir for clinically relevant carbapenemase- and 16s rrna methylase-producing enterobacteriaceae.the aim of this study was to evaluate wastewater for carbapenemase-producing enterobacteriaceae (cpe) and 16s rrna methylase-producing gram-negative bacteria (mpb) and to assess their occurrence following wastewater treatment. wastewater samples were collected between june 2015 and march 2016 in the sewage network of the city of basel (switzerland) from sites located before and after influx of wastewater from the hospital into the sewage network. samples were also obtained from the influent and ...201728668692
extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates from neonates in tunisia.this study was conducted to investigate extended-spectrum-β-lactamase (esbl) producing enterobacteriaceae isolates from the center of maternity and neonatology of monastir, tunisia. fourty-six strains out of 283 were found to produce esbl: klebsiella pneumoniae (n = 37), escherichia coli (n = 6), enterobacter cloacae (n = 2), and citrobacter freundi (n = 1). genotyping analysis, using eric2 and rapd, showed that strains were clonally unrelated. pcr amplification followed by sequencing revealed t ...201728648624
molecular and microbiological report of a hospital outbreak of ndm-1-carrying enterobacteriaceae in characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant enterobacteriaceae (cre) in a tertiary-care hospital in mexico.201728636666
genomic and phenotypic characterisation of fluoroquinolone resistance mechanisms in enterobacteriaceae in durban, south africa.resistance to fluoroquinolones (fq) is being increasingly reported and found to be mediated by efflux pumps, plasmid-mediated quinolone resistance genes (pmqr) and mutations in gyra, gyrb, parc and pare. however, studies reporting on fq resistance mechanisms (fqrm), particularly in africa, are focused mostly on salmonella. this study used a whole-genome-based approach to describe fqrm in forty-eight clinical enterobacteriaceae isolates comprising of klebsiella pneumoniae (n = 21), serratia marce ...201728636609
the bactericidal effect of simultaneous titanium oxide on common hospital bacteria.bacteria prevalence has increased in health centers and hospitals. infection control can decrease the number of bacteria. bacteria cause 90% of nosocomial infections and fungi, viruses, or protozoa are less involved. bacteria control can decrease the number of diseases. the simultaneous titanium oxide (smtio) is a new method of disinfection. this process degrades bacteria by producing hydroxyl radical. the present study was conducted with the aim of analyzing the bactericidal effect of simultane ...201728623574
dissemination of bla oxa-370 gene among several enterobacteriaceae species in brazil.oxa-370 is a recently described oxa-48 variant that has only been described in a few enterobacter spp. and klebsiella pneumoniae isolates. the purpose of this study is to assess the prevalence of oxa-370-producing isolates in carbapenem-nonsusceptible enterobacteriaceae recovered from 28 hospitals from brazil. real-time pcr was used to determine the presence of bla ndm-1, bla kpc-2, bla vim-type, bla ges-type, bla oxa-48-like, and bla imp-type genes. a total of 4,451 enterobacteriaceae were scre ...201728555403
sequencing of blaimp-carrying incn2 plasmids, and comparative genomics of incn2 plasmids harboring class 1 integrons.this work presents the complete nucleotide sequences of p0801-imp from klebsiella pneumoniae, p7121-imp from k. oxytoca, and p17285-imp from citrobacter freundii, which are recovered from three different cases of nosocomial infection. these three plasmids represent the first fully sequenced blaimp-carrying incn2 plasmids. further comparative genomics analysis of all the five integron-carrying incn2 plasmids p0801-imp, p7121-imp, p17285-imp, pjie137, and p34983-59.134kb indicates that they posses ...201728424761
igm-mediated autoimmune responses to oxidative specific epitopes, but not nitrosylated adducts, are significantly decreased in pregnancy: association with bacterial translocation, perinatal and lifetime major depression and the tryptophan catabolite (trycat) pathway.immunoglubulin (ig)m responses directed to oxidative specific epitopes (oses) and nitric oxide (no)-adducts are significantly associated with major depression and physio-somatic symptoms. end of term serum igm responses to oses and no-adducts were assayed in pregnant women with (n = 24) and without prenatal depression (n = 25) as well as in 24 non-pregnant women. associations of igm/iga responses to gram-negative gut commensal bacteria (leaky gut index) and iga/igm responses to tryptophan catabo ...201728600633
impact of a mixed educational and semi-restrictive antimicrobial stewardship project in a large teaching hospital in northern italy.the overuse of antimicrobials favors the dissemination of antimicrobial resistance, as well as invasive fungal diseases and clostridium difficile infections (cdi). in this study, we assessed the impact of a mixed educational and semi-restrictive antimicrobial stewardship (ams) project in a large teaching hospital in italy.201728856589
comparison of two whole-room uv-irradiation systems for enhanced disinfection of patient rooms contaminated with mrsa, carbapenemase-producing klebsiella pneumoniae and clostridium difficile spores.ultraviolet light decontamination systems are being used increasingly to supplement terminal disinfection of patient rooms. however efficacy may not be consistent in the presence of soil particularly against clostridium difficile spores.201728823547
response to letter of k. singh, 'role of silver nitrate in the efficacy of hydrogen peroxide aerial decontamination systems' regarding s. ali et al. 'efficacy of two hydrogen peroxide vapour aerial decontamination systems for enhanced disinfection of meticillin-resistant staphylococcus aureus, klebsiella pneumoniae and clostridium difficile in single isolation rooms'. 201728734723
prevalence and risk factors for colonization by clostridium difficile and extended-spectrum β-lactamase-producing enterobacteriaceae in rehabilitation clinics in germany.rehabilitation clinics may vary widely in terms of type of care provided, duration of hospital stay, and case severity. few data are available on prevalence of clostridium difficile or extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-e) colonization in rehabilitation clinics in germany.201728705583
the influence of maternal vaginal flora on the intestinal colonization in newborns and 3-month-old infants.the role of maternal vaginal bacteria on the colonization of neonatal gut is still a matter of discussion. our aim was to estimate the role of maternal vaginal flora on the development of intestinal flora in neonates and 3-month-old infants.201728420276
colonization by multidrug-resistant organisms in long-term care facilities in italy: a point-prevalence determine prevalence and risk factors for colonization by multidrug-resistant organisms (mdros) in long-term care facility (ltcf) residents in italy. genotypes of mdro isolates were investigated.201728412380
conformational control of udp-galactopyranose mutase inhibition.udp-galactopyranose mutase (glf or ugm) catalyzes the formation of uridine 5'-diphosphate-α-d-galactofuranose (udp-galf) from udp-galactopyranose (udp-galp). the enzyme is required for the production of galf-containing glycans. ugm is absent in mammals, but members of the corynebacterineae suborder require ugm for cell envelope biosynthesis. the need for ugm in some pathogens has prompted the search for inhibitors that could serve as antibiotic leads. optimizing inhibitor potency, however, has b ...201728608671
enhanced larvicidal, antibacterial, and photocatalytic efficacy of tio2 nanohybrids green synthesized using the aqueous leaf extract of parthenium hysterophorus.titanium dioxide nanoparticles are emerging as a biocompatible nanomaterial with multipurpose bioactivities. in this study, titanium dioxide (tio2) nanoparticles were effectively synthesized using the aqueous leaf extracts of parthenium hysterophorus prepared by microwave irradiation. tio2 nanoparticles were fabricated by treating the p. hysterophorus leaf extracts with the tio4 solution. biologically active compounds such as alcohols, phenols, alkanes, and fluoroalkanes were involved in bioredu ...201728537028
the clear and present danger of carbapenemase-producing enterobacteriaceae (cpe) in new zealand: time for a national response plan.antimicrobial resistance (amr) in general poses a threat to the sustainability of modern healthcare, but a particularly urgent and serious threat is posed by a specific group of antibiotic-resistant bacteria known as carbapenemase-producing enterobacteriaceae (cpe). cpe are resistant to nearly all antibiotics and include common pathogens such as escherichia coli and klebsiella pneumoniae. in new zealand, the incidence of cpe has increased from three isolates in 2012 to 45 in 2016. the current ep ...201728449019
colistin resistance associated with outer membrane protein change in klebsiella pneumoniae and enterobacter this study, outer membrane proteins (omps) of colistin-resistant klebsiella pneumoniae and enterobacter asburiae were analyzed. one colistin-susceptible and three colistin-resistant k. pneumoniae sequence type 258 strains as well as one colistin-susceptible e. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. omp analysis of each strain was performed by microchip method. matrix-assisted laser desorption ionization time of flight/mass spectrometry (maldi- ...201728629229
isolation and identification of nonoral pathogenic bacteria in the oral cavity of patients with removable dentures.dentures in the oral cavity may act as a reservoir of microorganisms, which may be related to systemic infections. the aim of this study was to investigate the nonoral pathogenic bacteria in the oral cavity of patients with removable dentures in shiraz, southern iran.201728852636
antimicrobial activity of ceftazidime-avibactam tested against multidrug-resistant enterobacteriaceae and pseudomonas aeruginosa isolates from united states medical centers (2013--2016).the in vitro activity of ceftazidime-avibactam and many comparator agents was determined against various resistant subsets of organisms selected among 36,380 enterobacteriaceae and 7,868 pseudomonas aeruginosa. isolates were consecutively collected from 94 us hospitals, and all isolates were tested for susceptibility by reference broth microdilution methods in a central monitoring laboratory (jmi laboratories). enterobacteriaceae isolates resistant to carbapenems (cre) and/or ceftazidime-avibact ...201728827415
a simple phenotypic method for screening of mcr-1-mediated colistin evaluate a novel method, named colistin-mac test, for phenotypic screening of acquired colistin-resistance mediated by transferable mcr-1 resistance determinants, based on colistin mic reduction in the presence of dipicolinic acid (dpa).201728827120
rapid phenotypic stress-based microfluidic antibiotic susceptibility testing of gram-negative clinical isolates.bacteremia is a life-threatening condition for which antibiotics must be prescribed within hours of clinical diagnosis. since the current gold standard for bacteremia diagnosis is based on conventional methods developed in the mid-1800s-growth on agar or in broth-identification and susceptibility profiling for both gram-positive and gram-negative bacterial species requires at least 48-72 h. recent advancements in accelerated phenotypic antibiotic susceptibility testing have centered on the micro ...201728808348
the first enterobacter cloacae co-producing ndm and oxa-48 carbapenemases and interhospital spread of oxa-48 and ndm-producing klebsiella pneumoniae in turkey.the aim of this study was to investigate the occurrence of carbapenemase-producing enterobacteriaceae.201728792697
the occurrence of ctx-m-25-producing enterobacteriaceae in day-old broiler chicks in chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between january and july 2016. sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant enterobacteriaceae. cefotaxime (ctx)-resistant klebsiella pneumoniae (1 isolate) and enterobacter cloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples. conjugation experiments revealed that th ...201728781343
antimicrobial resistance of 3 types of gram-negative bacteria isolated from hospital surfaces and the hands of health care workers.there has been an increased focus in recent years on antimicrobial resistance of bacteria isolated from clinical samples. however, resistance of bacteria from hospital environments has been less frequently investigated.201728780198
photoinactivation of multidrug resistant bacteria by monomeric methylene blue conjugated gold nanoparticles.multidrug resistant (mdr) bacterial infections have become a severe threat to the community health due to a progressive rise in antibiotic resistance. nanoparticle-based photodynamic therapy (pdt) is increasingly been adopted as a potential antimicrobial option, yet the cytotoxicity associated with pdt is quite unspecific. herein, we show concanavalin-a (cona) directed dextran capped gold nanoparticles (gnpdex-cona) enhanced the efficacy and selectivity of methylene blue (mb) induced killing of ...201728778019
antimicrobial effects of lysophosphatidylcholine on methicillin-resistant staphylococcus aureus.methicillin-resistant staphylococcus aureus (mrsa) is an important health care-associated and community-associated pathogen and causes a large number of infections worldwide. for the purpose of application to topical treatment of mrsa infection, we examined the antimicrobial effects of lysophosphatidylcholine (lpc) on mrsa strains. we also investigated the combination effect of lpc and gentamicin on mrsa growth.201728748087
introduction of sample tubes with sodium azide as a preservative for ethyl glucuronide in urine.ethyl glucuronide (etg) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. however, the instability of etg in urine against bacterial degradation or the post-collectional synthesis of etg in contaminated samples may cause false interpretation of etg results in urine samples. this study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism ta ...201728712037
multidrug-resistant enterobacteriaceae in lebanese hospital wastewater: implication in the one health concept.extended-spectrum beta-lactamases (esbl) and carbapenemase-producing organisms pose severe problems for hospitalized patients. in the absence of efficient sanitation and sewage disposal, the risks for transmission of hospital organisms into the community are high. our objectives were to study the occurrence and mechanisms of resistance of multidrug-resistant gram-negative bacilli in two lebanese hospital sewage treatment plants. wastewater samples were collected, filtered, and cultivated on macc ...201728650688
fecal carriage of extended-spectrum β-lactamase- and carbapenemase-producing enterobacteriaceae in egyptian patients with community-onset gastrointestinal complaints: a hospital -based cross-sectional study.the aim of this study was to determine the prevalence of extended-spectrum β-lactamase (esbl) and carbapenemase production among enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to el-ahrar general hospital, zagazig, egypt in the period between january 2013 and may 2013.201728630686
population pharmacokinetics of tigecycline in critically ill patients with severe infections.we sought to describe the population pharmacokinetics of tigecycline in critically ill patients and to determine optimized dosing regimens of tigecycline for different bacterial infections. this prospective study included 10 critically ill patients given a standard dose of tigecycline. blood samples were collected during one dosing interval and were analyzed using validated chromatography. population pharmacokinetics and monte carlo dosing simulations were undertaken using pmetrics. three target ...201728607024
genomic epidemiology of global vim-producing data on the molecular epidemiology of enterobacteriaceae with vim carbapenemases are limited.201728520983
clinical and molecular epidemiology of carbapenem-resistant enterobacteriaceae among adult inpatients in singapore.since 2010, the incidence of carbapenem-resistant enterobacteriaceae (cre) has been increasing in singapore. we analyzed the clinical and molecular epidemiology of cre among adult inpatients in singapore.201728475792
transfer of kpc-2 carbapenemase from klebsiella pneumoniae to enterobacter cloacae in a patient receiving meropenem therapy.the horizontal transfer of a plasmid bearing the blakpc-2 gene from k. pneumoniae to e. cloacae infecting the respiratory tract of a patient during meropenem therapy was elucidated. this finding is particularly worrisome, since these drugs are of last resort for multidrug-resistant gram-negative pathogens.201728442305
allodemic distribution of plasmids co-harbouring blactx-m-15/aac(6')-ib-cr/qnrb in klebsiella pneumoniae is the main source of extended-spectrum β-lactamases in uruguay's paediatric hospital.this study aimed to describe the characteristics of clinical isolates of extended-spectrum β-lactamase (esbl)-producing enterobacteria (epe) in uruguay's paediatric hospital.201728419869
carbapenem-resistant enterobacter cloacae in patients from the us veterans health administration, 2006-2015.we analyzed carbapenem-resistant enterobacteriaceae (cre) trends among patients from the us veterans health administration (vha). after the emergence of cre in the eastern united states, resistance rates remained stable in klebsiella pneumoniae but increased in enterobacter cloacae complex, suggesting a "second epidemic". vha offers a vantage point for monitoring nationwide cre trends.201728418318
oxa-48-like carbapenemases in the uk: an analysis of isolates and cases from 2007 to 2014.oxa-48-like carbapenemases have spread worldwide since 2001. we analysed patient and microbiological data for uk isolates with these enzymes as confirmed by the national reference laboratory from november 2007 to december 2014.201728199647
factors associated with carriage of carbapenem-non-susceptible enterobacteriaceaein north-eastern france and outcomes of infected patients.carbapenems are frequently used as a last resort to treat infections caused by multidrug-resistant gram-negative organisms, thus carbapenem-non-susceptible enterobacteriaceae (cnse) is an emerging health threat.201728158526
accelerated detection of extended-spectrum β-lactamases in clinical isolates of enterobacteriaceae.a prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum β-lactamases (esbls) in clinical isolates of escherichia coli, klebsiella pneumoniae and other gram-negative bacteria. a modified double-disc test (mddt) is incorporated in a gram-negative template for routine susceptibility testing. the mddt identified accurately esbls in all isolates subsequently confirmed as esblproducers by the standard clinical laboratory standards ...200628700883
antibacterial properties of nonwoven wound dressings coated with manuka honey or methylglyoxal.manuka honey (mh) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. mh provides unique antibacterial activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (mgo). aiming to investigate an antibiotic-free antimicrobial strategy, we studied the antibacterial activity of both mh and mgo (at equivalent mgo concentrations) when applied as a physical coating to a nonwoven fabric wound dress ...201728813014
urinary tract infections after kidney transplantation: a risk factor analysis of 417 patients.background the aim of this study was to evaluate the number of urinary tract infections (uti) that occur after kidney transplantation (kt) and to identify possible risk factors for development of a uti. material and methods we retrospectively analyzed all kts performed between january 2012 and december 2013 in the erasmus university medical center, rotterdam. uti was scored if: (1) a patient had a urine culture with no more than 2 species of microorganisms, (2) at least 1 of which was a bacteriu ...201728663538
spectrum and antibiotic resistance of uropathogens between 2004 and 2015 in a tertiary care hospital in hungary.surveillance of the bacterial spectrum and antibiotic-resistance patterns of locally occurring uropathogens is essential to serve as a basis for empirical treatment of urinary tract infections (utis), as antibiotic-resistance rates may vary geographically with significant differences between countries and regions, and with time.201728621642
bacterial sepsis in neonates: single centre study in a neonatal intensive care unit in bosnia and herzegovina.the aim of the study was to evaluate the incidence, mortality, risk factors, aetiology and the susceptibility to antibiotics of the bacteria responsible for sepsis.201728605923
collaborative research on puerperal infections in bangladesh.bangladesh is considered as a high-risk country for emerging infectious diseases because of its high population density, poverty, and unhygienic conditions. although control efforts have primarily been focused on major infectious diseases such as diarrheal diseases, tuberculosis, malaria, and hiv infection, the prevalence and impact of many local or minor infectious diseases are still unclarified in this country. in this review, we present our recent experience and outcomes of collaborative rese ...201728552890
effect of gnp functionalisation and multiple n-methylation of β-amyloid residue (32-37) on gram-positive the previous report, the authors showed the gold nanoparticle (gnp) functionalised multiple n-methylated fragments of the residue (32-37) of beta (β)-amyloid protein (1-42), cggiglmvg and cgggggiglmvg toward disruption of β-amyloid (1-42), the predominant component of senile plaques. herein the in vitro antimicrobial activities of both normal and multiple n-methylated sequences of cggiglmvg and cgggggiglmvg were screened and it was found that all the eight sequences including four (non-functi ...201728530185
development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (rpa) assays for detection of common bacterial urinary tract infection (uti) pathogens.201728510991
antimicrobial resistance patterns in women with positive urine culture: does menopausal status make a significant difference?aim. urinary tract infection (uti) is considered one of the most common bacterial infections in women. the aim of this study was to investigate the types of uropathogens present, as well as the degree of antimicrobial drug resistance seen among premenopausal (n = 2748) and postmenopausal (n = 1705) women with uncomplicated uti. methods. urinary samples (n = 4453) collected from women with uti were analyzed in terms of uropathogens present. these were considered as positive if bacterial growth wa ...201728497048
applications of copolymer for rapid identification of bacteria in blood culture broths using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) can be used to identify pathogens in blood culture samples. however, sample pretreatment is needed for direct identification of microbes in blood culture bottles. conventional protocols are complex and time-consuming. therefore, in this study, we developed a method for collecting bacteria using polyallylamine-polystyrene copolymer for application in wastewater treatment technology. using representative ba ...201728461023
bacterial epidemiology and antimicrobial resistance profiles of urinary specimens of the elderly.although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles.201728408060
low prevalence of carbapenem-resistant enterobacteriaceae among wounded military personnel.multidrug-resistant organisms (mdros) are a global health problem that affect both civilian and military populations. among wounded warriors, mdros further complicate the care of trauma-related infections, resulting in extended duration of hospitalization, as well as increased morbidity and mortality. during the wars in iraq and afghanistan, extended spectrum β-lactamase-producing enterobacteriaceae were frequently isolated from wounded warriors. the potential emergence of difficult-to-treat car ...201728853114
photodynamic inactivation of multiresistant bacteria (kpc) using zinc(ii)phthalocyanines.the worldwide increase in antibiotic resistance has led to search of alternatives anti-microbial therapies such as photodynamic inactivation. the aim of this paper was to evaluate the photodynamic activity in vitro of a neutral and two cationic zn phthalocyanines. their photokilling activity was tested on escherichia coli atcc 25922 and klebsiella pneumoniae carbapenemase (kpc)-producing. after treating bacteria with phthalocyanines, the cultures were irradiated with white light. as a result, th ...201728844390
clinical characteristics of bloodstream infections in pediatric acute leukemia: a single-center experience with 231 patients.acute leukemia is the most common pediatric hematological malignancy. bloodstream infections (bsis) are severe complications in these patients during chemotherapy. this study aimed to explore the clinical presentation and etiology of bsi, as well as the common sites of infection, and to provide a basis for the rational regarding antibiotic use.201728836551
trends in antimicrobial resistance against enterobacteriaceae strains isolated from blood: a 10-year epidemiological study in mainland china (2004-2014).antimicrobial resistance is a serious problem that compromises the empirical treatment of infections, resulting in a lack of effective antibiotics and high medical expenses. here, we aimed to monitor the trends in antimicrobial resistance among enterobacteriaceae isolated from blood samples in mainland china.201728836547
a case of emphysematous cystitis caused by klebsiella pneumoniae.emphysematous cystitis is a rare type of urinary tract infection that is characterized by air pockets within the bladder wall and lumen, which come from gas that is mainly produced by gram-negative bacteria, notably escherichia coli. this infection is more common in older women with poorly controlled diabetes. an abdominal computerized tomography (ct) scan is the gold standard method to make the diagnosis. the infection can be life-threatening, so prompt treatment is essential. we present a case ...201728832314
incidence of cross infection in liver transplant patients. risk factors and role of determine the incidence and risk factors of infection in liver transplant recipients immediately post-transplant, during admission to a liver transplant unit during the immediate post-transplant admission.201728822713
ultrahigh antibacterial efficacy of meropenem-loaded chitosan nanoparticles in a septic animal model.antimicrobial resistance is one of the most significant health challenges worldwide. meropenem is a broad spectrum beta-lactam antibiotic that possesses high activity against gram-positive and gram-negative bacteria. however, it has a short plasma half-life, and thus requiring frequent administration of high doses. for the first time, meropenem-loaded chitosan nanoparticles were prepared and evaluated as a potential tool to overcome antimicrobial resistance and to improve pharmacokinetics of the ...201728821026
prevalence study of plasmid-mediated ampc β-lactamases in enterobacteriaceae lacking inducible ampc from saudi hospitals.enterobacteriaceae encoding plasmid-mediated ampc (pampc) β-lactamases confer resistance to the third generation cephalosporins. pampc association with extended spectrum β-lactamases (esbls), plasmid-mediated quinolone resistance (pmqr) and aminoglycoside modifying enzymes (ames) is well documented. there are limited data regarding the epidemiology and clinical significance of pampc in saudi arabia. this study aimed to determine the prevalence of pampc and its coexistence with esbls, pmqr and am ...201728820112
novel consortium of klebsiella variicola and lactobacillus species enhances the functional potential of fermented dairy products by increasing the availability of branched-chain amino acids and the amount of distinctive volatiles.identify novel bacterial taxa that could increase the availability of branched-chain amino acids and the amount of distinctive volatiles during skim milk fermentation.201728815819
effect of poly-hexamethylene biguanide hydrochloride (phmb) treated non-sterile medical gloves upon the transmission of streptococcus pyogenes, carbapenem-resistant e. coli, mrsa and klebsiella pneumoniae from contact surfaces.reduction of accidental contamination of the near-patient environment has potential to reduce acquisition of healthcare-associated infection(s). although medical gloves should be removed when soiled or touching the environment, compliance is variable. the use of antimicrobial-impregnated medical gloves could reduce the horizontal-transfer of bacterial contamination between surfaces.201728814284
dipicolinic acid derivatives as inhibitors of new delhi metallo-β-lactamase-1.the efficacy of β-lactam antibiotics is threatened by the emergence and global spread of metallo-β-lactamase (mbl) mediated resistance, specifically new delhi metallo-β-lactamase-1 (ndm-1). by utilization of fragment-based drug discovery (fbdd), a new class of inhibitors for ndm-1 and two related β-lactamases, imp-1 and vim-2, was identified. on the basis of 2,6-dipicolinic acid (dpa), several libraries were synthesized for structure-activity relationship (sar) analysis. inhibitor 36 (ic50 = 80 ...201728809565
p1220-ctxm, a pkp048-related incfiik plasmid carrying bla ctx-m-14 and qnrb4.this study aimed to characterize plasmid-mediated antimicrobial resistance in clinical klebsiella pneumoniae 1220 carrying bla ctx-m-14 and qnrb4.201728799786
evolving beta-lactamase epidemiology in enterobacteriaceae from italian nationwide surveillance, october 2013: kpc-carbapenemase spreading among outpatients.extended-spectrum beta-lactamases (esbls), ampc-type beta-lactamases (acbls) and carbapenemases are among the most important resistance mechanisms in enterobacteriaceae. this study investigated the presence of these resistance mechanisms in consecutive non-replicate isolates of escherichia coli (n = 2,352), klebsiella pneumoniae (n = 697), and proteus mirabilis (n = 275) from an italian nationwide cross-sectional survey carried out in october 2013. overall, 15.3% of isolates were non-susceptible ...201728797330
prevalence of antimicrobial drug resistant bacteria carried by in- and outpatients attending a secondary care hospital in zambia.antimicrobial resistance is an increasing global health problem. very little data on resistance patterns of pathogenic bacteria in low-income countries exist. the aim of this study was to measure the prevalence of antimicrobial drug resistant bacteria carried by in- and outpatients in the resource constraint setting of a secondary care hospital in zambia. nasal and rectal samples from 50 in- and 50 outpatients were collected. patients were randomly selected and informed consent was obtained. nas ...201728797299
geographical variation in therapy for bloodstream infections due to multidrug-resistant enterobacteriaceae: a post hoc analysis of the increment study.we aimed to describe regional differences in therapy for bloodstream infection (bsi) caused by extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-e) or carbapenemase-producing enterobacteriaceae (cpe). 1,482 patients in 12 countries were included from an observational study of bsi caused by esbl-e or cpe. multivariate logistic regression was used to calculate adjusted odds ratios (aors) for the influence of country of recruitment on empirical use of β-lactam/β-lactamase inhibitors ...201728782704
impact of dust exposure on mixed bacterial cultures and during eukaryotic cell co-culture infections.on a daily basis, humans, and their colonizing microbiome, are exposed to both indoor and outdoor dust, containing both deleterious organic and inorganic contaminants, through dermal contact, inhalation, and ingestion. recent studies evaluating the dust exposure responses of opportunistic pathogens, such as escherichia coli and pseudomonas aeruginosa, revealed significant increases in biofilm formation following dust exposure. in this study, the effects of dust exposure on mixed bacterial cultur ...201728776099
high frequency of the combined presence of qrdr mutations and pmqr determinants in multidrug-resistant klebsiella pneumoniae and escherichia coli isolates from nosocomial and community-acquired infections.plasmid-mediated quinolone resistance (pmqr) determinants combined with mutations in quinolone resistance-determining regions (qrdrs) and clonal dissemination were investigated in 40 fluoroquinolone-resistant klebsiellapneumoniae and escherichiacoli isolates from nosocomial and community-acquired infections. we observed nucleotide substitutions in gyra (ser83ile, val37leu, lys154arg, ser171ala, ser19asn, ile198val, ser83tyr, ser83leu, asp87asn and asp87gly) and parc genes (ser80ile, glu84lys, al ...201728771139
determinants of implementation of isolation precautions against infections by multidrug-resistant microorganisms: a hospital-based, multicenter, observational study.objectives we aimed to ascertain the factors associated with lack of isolation precautions (ip) in patients infected or colonized by third-generation cephalosporin-resistant enterobacteriaceae (3gcr-e) and methicillin-resistant staphylococcus aureus (mrsa) in hospital settings. design prospective surveillance and audit of practices. setting the study included 4 university hospitals in lyon, france. participants all patients hospitalized between april and june in 2013 and 2015 were included. case ...201728758615
biological activities and secondary metabolite screening of rumex hastatus extract through fourier transform infrared and raman spectroscopy.human infectious diseases began by various pathogens including bacteria, fungi, viruses, parasites, and protozoans. these infectious agents are controlled by using synthetic drugs as well as natural sources.201728758575
nosocomial bacterial infections and their antimicrobial susceptibility patterns among patients in ugandan intensive care units: a cross sectional study.the intensive care unit (icu) admits critically ill patients requiring advanced airway, respiratory, cardiac and renal support. despite the highly-specialized interventions, the mortality and morbidity is still high due to a number of reasons including nosocomial infections, which are the most likely complications in hospitalized patients with the rates being highest among icu patients.201728754148
intra-abdominal infection after radical gastrectomy for gastric cancer: incidence, pathogens, risk factors and outcomes.surgical site infection, particularly intra-abdominal infection (iai), remains a clinically important event after gastrectomy for gastric cancer. the aim of this retrospective study was to clarify the incidence, pathogens, risk factors and outcomes of iai following gastrectomy for gastric cancer.201728751223
staphylococcus aureus single-stranded dna-binding protein ssba can bind but cannot stimulate pria helicase.single-stranded dna-binding protein (ssb) and pria helicase play important roles in bacterial dna replication restart process. the mechanism by which pria helicase is bound and stimulated by ssb in escherichia coli (ec) has been established, but information on this process in gram-positive bacteria are limited. we characterized the properties of ssb from staphylococcus aureus (sassba, a counterpart of ecssb) and analyzed its interaction with sapria. the gel filtration chromatography analysis of ...201728750050
competition is the basis of the transport mechanism of the nhab na+/h+ exchanger from klebsiella exchange is essential for survival of all organisms, having a role in the regulation of the intracellular na+ concentration, ph and cell volume. furthermore, na+/h+ exchangers were shown to be involved in the virulence of the bacterium yersinia pestis, indicating they might be potential targets for novel antibiotic treatments. the model system for na+/h+ exchangers is the nhaa transporter from escherichia coli, ecnhaa. therefore, the general transport mechanism of nhaa exchangers is curre ...201728750048
biomimetic synthesis of agnps from penicillium chrysogenum strain fgcc/bls1 by optimising physico-cultural conditions and assessment of their antimicrobial potential.biomimetic synthesis of metal nanoparticles (nps) is safe and eco-friendly; therefore, find diverse applications. considering this, the soil fungi penicillium chrysogenum strain fungal germplasm collection centre/ bls1 was isolated, characterized and explored to synthesize extracellular silver nps (agnps) under optimised conditions. the synthesis of agnps was investigated using ultraviolet (uv)-visible spectroscopy, fourier-transform infra-red spectroscopy (ftir), transmission electron microscop ...201728745292
esbl-producing escherichia coli and klebsiella pneumoniae: the most prevalent clinical isolates obtained between 2005 and 2012 in identify the prevalence of esbl genes in the principal group of enterobacteriaceae causing nosocomial infections and to identify the phylogenetic group in escherichia coli isolates.201728739224
detection of multidrug resistant (mdr) bacteria in untreated waste water disposals of hospitals in dhaka city, bangladesh.emergence of mdr bacteria is a global problem and a major burden for treatment of various infectious diseases. this study was performed to detect antibiotic resistant bacteria in untreated hospital waste.201728729208
antimicrobial resistance patterns of gram-negative bacteria isolated from bloodstream infections in an iranian referral pediatric hospital: a six-year study.bloodstream infections (bsis) are considered as a major cause of morbidity and mortality in children all over the world. the aim of this study was to describe the epidemiology and antimicrobial resistance patterns of gram-negative bacteria (gnb) isolated from bloodstream infections (bsis) in children admitted to the children medical center hospital (cmc), tehran, iran.201728729206
[pyogenic liver abscess: changes of organisms and consequences for diagnosis and therapy].liver abscess is a rare but potentially fatal disease with mortality rates of 2 - 12 %. the spectrum of causative microorganisms varies across the world and is changing due to increasing worldwide travel activity. as a result, previously less frequent pathogens must increasingly be considered in the differential diagnosis. the most common pathogens of liver abscess are so-called abscess streptococci, anaerobes and gram-negative bacteria such as escherichia coli and klebsiella pneumoniae. certain ...201728728202
mcr-1 and blakpc-3 in escherichia coli sequence type 744 after meropenem and colistin therapy, portugal.escherichia coli ec36 was recovered from a patient in portugal after treatment with meropenem and colistin. besides an incf plasmid with tn1441d-blakpc-3, already reported in clinical strains in this country, e. coli ec36 co-harbored an incx4::mcr-1 gene. results highlight emerging co-resistance to carbapenems and polymyxins after therapy with drugs from both classes.201728726622
serum albumin and osmolality inhibit bdellovibrio bacteriovorus predation in human serum.we evaluated the bactericidal activity of bdellovibrio bacteriovorus, strain hd100, within blood sera against bacterial strains commonly associated with bacteremic infections, including e. coli, klebsiella pneumoniae and salmonella enterica. tests show that b. bacteriovorus hd100 is not susceptible to serum complement or its bactericidal activity. after a two hour exposure to human sera, the prey populations decreased 15- to 7,300-fold due to the serum complement activity while, in contrast, the ...201728725056
genomic epidemiology of global klebsiella pneumoniae carbapenemase (kpc)-producing escherichia coli.the dissemination of carbapenem resistance in escherichia coli has major implications for the management of common infections. bla kpc, encoding a transmissible carbapenemase (kpc), has historically largely been associated with klebsiella pneumoniae, a predominant plasmid (pkpqil), and a specific transposable element (tn4401, ~10 kb). here we characterize the genetic features of bla kpc emergence in global e. coli, 2008-2013, using both long- and short-read whole-genome sequencing. amongst 43/45 ...201728725045
isolation and in vitro evaluation of bacteriophages against mdr-bacterial isolates from septic wound infections.multi-drug resistance has become a major problem for the treatment of pathogenic bacterial infections. the use of bacteriophages is an attractive approach to overcome the problem of drug resistance in several pathogens that cause fatal diseases. our study aimed to isolate multi drug resistant bacteria from patients with septic wounds and then isolate and apply bacteriophages in vitro as alternative therapeutic agents. pus samples were aseptically collected from rajiv gandhi institute of medical ...201728719657
a novel antibacterial peptide derived from crocodylus siamensis hemoglobin hydrolysate induces membrane permeabilisation causing iron dysregulation, oxidative stress and bacterial death.a novel antibacterial peptide from crocodylus siamensis hemoglobin hydrolysate (chhs) was characterised for antimicrobial activity.201728712131
a long-term study of the diversity of oxa-48-like carbapenemase-producing bacterial strains in infected patients and carriers.since their emergence at the beginning of the century, oxa-48 carbapenemases have spread in the community and in hospitals. to assess the diversity of oxa-48-producing bacterial strains and plasmids in the hospital setting, we studied the strains isolated from patients in three hospitals in the paris area.201728708476
activity of temocillin against ctx-m-producing escherichia coli and klebsiella pneumoniae from germany. 201728705669
in-vitro activity of ceftolozane/tazobactam against pseudomonas aeruginosa and enterobacteriaceae isolates recovered from hospitalized patients in evaluate the activity of ceftolozane/tazobactam in comparison with other broad-spectrum antimicrobials against pseudomonas aeruginosa and enterobacteriaceae, 497 non-duplicate p. aeruginosa and 802 enterobacteriaceae clinical isolates were consecutively collected during the period from september 2014 to april 2015 from patients in germany with bloodstream, lower respiratory tract, intraabdominal or urinary tract infections. antimicrobial susceptibility testing was performed by broth microdilu ...201728705666
silver nanoparticles functionalized with ampicillin.the resistance of pathogenic bacteria to antibiotics has become a serious problem. the emphasis is placed on the development of new, effective antimicrobial strategies. one of them is the use of agnps in association with antibiotic drugs. the aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high-performance liquid chromatography approach. to confirm the occurrence of silver nanopartic ...201728704596
assessing antibiotic therapy effectiveness against the major bacterial pathogens in a hospital using an integrated assess the effectiveness of antibiotic therapy against five indicator bacteria in a chinese hospital using an index-based approach.201728699775
in vitro activity of ceftolozane/tazobactam in combination with other classes of antibacterial agents.combination antibiotic therapy has been used successfully to treat some patients with bacterial infections. however, although certain combinations may result in beneficial synergistic activity, others may produce antagonistic effects resulting in poor treatment outcomes. ceftolozane/tazobactam is an antibacterial agent with potent activity against pseudomonas aeruginosa and many other gram-negative pathogens, including extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae. this study ...201728689923
chitosans inhibit the growth and the adhesion of klebsiella pneumoniae and escherichia coli clinical isolates on urinary catheters.the aim of this study was to determine the antimicrobial activity of different chitosans (cs) against typical colonizing pathogens of the urinary tract and to assess their efficacy against bacterial adhesion and the subsequent biofilm formation on urinary catheters.201728689873
prevalence and drug resistance characteristics of carbapenem-resistant enterobacteriaceae in hangzhou, china.with the abuse of antimicrobial agents in developing countries, increasing number of carbapenem-resistant enterobacteriaceae (cre) attracted considerable public concern. a retrospective study was conducted based on 242 cre strains from a tertiary hospital in hangzhou, china to investigate prevalence and drug resistance characteristics of cre in southeast china. bacterial species were identified. antimicrobial susceptibility was examined by broth microdilution method or epsilometer test. resistan ...201728687975
molecular epidemiology of colistin-resistant enterobacteriaceae in inpatients and avian from china: high prevalence of mcr-negative klebsiella pneumoniae.whether chromosomal and transmissible mechanisms contribute simultaneously to colistin resistance in klebsiella pneumoniae and escherichia coli remains unknown. this study aims to identify the underlying mechanisms of colistin-resistance in inpatient and avian k. pneumoniae and e. coli in china. we retrospectively screened 2353 enterobacteriaceae isolates from inpatients at multi-centers during 2011-2014, and 168 avian isolates from one slaughterhouse in 2013 for the presence of mcr-1/mcr-2. mut ...201728668693
propensity score matched analysis comparing the clinical outcome of klebsiella pneumoniae and escherichia coli causing community-onset monomicrobial bacteremia.bacteremia is a life-threatening condition that is associated with substantial healthcare costs. escherichia coli and klebsiella pneumoniae are the leading causes of community-onset gram-negative bacteremia. however, a comprehensive comparison between these pathogens involved in bacteremia episodes has yet to be this retrospective cohort study, adults with community-onset monomicrobial bacteremia caused by e coli or k pneumoniae were recruited in the emergency department of a medical ...201728658101
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