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probiotic properties of lactobacillus fermentum strains isolated from human oral samples and description of their antibacterial activity.gram positive bacteria produce peptides, defined bacteriocins which exhibit good antibacterial activity.201628034294
etiological profile of early neonatal bacterial sepsis by multiplex qpcr.given the major impact in terms of morbidity and mortality that episodes of early neonatal sepsis (ens) have on both newborns and health systems, this study aimed to identify the etiological profile of early neonatal bacterial sepsis by a multiplex quantitative real-time polymerase chain reaction (qpcr).201628036311
antimicrobial resistance of selected invasive bacteria in a tertiary care center: results of a prospective surveillance study.we aimed to report the distribution and resistance patterns of eight invasive clinically relevant bacteria surveyed in the clinical center of serbia (ccs) in belgrade.201628036312
etiology of early onset septicemia among neonates at the university college hospital, ibadan, nigeria.neonatal septicemia remains a major cause of newborn deaths in developing countries. its burden is further compounded by the emergence of multidrug-resistant pathogens, which is related to a lack of antibiotic protocols resulting in unrestricted use of antibiotics. the absence of reliable antibiotic sensitivity testing makes the formulation of antibiotic guidelines and judicious use of antibiotics difficult. this study sought to identify the current bacterial agents associated with early onset s ...201628036314
rectal carriage of extended-spectrum β-lactamase- and carbapenemase-producing enterobacteriaceae among hospitalised neonates in a neonatal intensive care unit in fez, morocco.the aim of this study was to investigate the faecal carriage and molecular epidemiology of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esble) isolated from rectal samples of neonates hospitalised in a neonatal intensive care unit (nicu) of a university hospital in fez, morocco.201728039104
the pharmacodynamics of avibactam in combination with ceftaroline or ceftazidime against β-lactamase-producing enterobacteriaceae studied in an in vitro model of infection.pharmacodynamics of β-lactamase inhibitors are an area of intense interest as new β-lactam/β-lactamase inhibitor combinations enter clinical development and clinical practice. avibactam, a non-β-lactam β-lactamase inhibitor, has been combined with ceftaroline or ceftazidime but these two combinations have not been directly compared.201728039276
polymyxin b in combination with meropenem against carbapenemase-producing klebsiella pneumoniae: pharmacodynamics and morphological changes.combination therapy provides a useful therapeutic approach to overcome resistance until new antibiotics become available. in this study, the pharmacodynamics, including the morphological effects, of polymyxin b (pmb) and meropenem alone and in combination against kpc-producing klebsiella pneumoniae clinical isolates was examined. ten clinical isolates were obtained from patients undergoing treatment for mediastinitis. kpcs were identified and mics were measured using microbroth dilution. time-ki ...201728040408
proteomic profile of susceptible and multidrug-resistant clinical isolates of escherichia coli and klebsiella pneumoniae using label-free and immunoproteomic strategies.infectious diseases caused by multidrug-resistant (mdr) enterobacteriaceae have exponentially increased in the past decade, and are a major concern in hospitals. in the first part of the work, we compared the proteome profile of mdr and susceptible clinical isolates of escherichia coli and klebsiella pneumoniae in order to identify possible biological processes associated with drug resistance and susceptible phenotypes, using a label-free approach. in the second part, we used an immunoproteomics ...201728040467
metabolic engineering of klebsiella pneumoniae based on in silico analysis and its pilot-scale application for 1,3-propanediol and 2,3-butanediol co-production.klebsiella pneumoniae naturally produces relatively large amounts of 1,3-propanediol (1,3-pd) and 2,3-butanediol (2,3-bd) along with various byproducts using glycerol as a carbon source. the ldha and mdh genes in k. pneumoniae were deleted based on its in silico gene knockout simulation with the criteria of maximizing 1,3-pd and 2,3-bd production and minimizing byproducts formation and cell growth retardation. in addition, the agitation speed, which is known to strongly affect 1,3-pd and 2,3-bd ...201728040869
determination of minimum biofilm eradication concentrations of orbifloxacin for canine bacterial uropathogens over different treatment periods.biofilm formation can cause refractory urinary tract infections (utis) in dogs; however, minimum biofilm eradication concentrations (mbecs) of veterinary drugs against canine uropathogens remain to be investigated. in this study, the mbecs of orbifloxacin (obfx), trimethoprim-sulfamethoxazole (tms) and amoxicillin/clavulanate (acv) over different time periods for treatment of canine uropathogenic escherichia coli (n = 10) were determined. the mbecs of obfx for other bacterial uropathogens, inclu ...201728042660
tigecycline therapy for nosocomial pneumonia due to carbapenem-resistant gram-negative bacteria in critically ill patients who received inappropriate initial antibiotic treatment: a retrospective case study.background. nosocomial pneumonia due to carbapenem-resistant gram-negative bacteria (crgnb) is a growing concern because treatment options are limited and the mortality rate is high. the effect of tigecycline (tgc) on nosocomial pneumonia due to crgnb in patients who have received inappropriate initial empiric antibiotic treatment (iiat) is unclear. therefore, this study aimed to examine the effect of tgc on nosocomial pneumonia due to crgnb in critically ill patients who had received iiat. meth ...201628044137
liver abscess and metastatic endophthalmitis secondary to klebsiella pneumoniae k1. 201728050045
in vitro evaluation of the antimicrobial ability and cytotoxicity on two melanoma cell lines of a benzylamide derivative of maslinic acid.maslinic acid is a pentacyclic triterpene extracted from olives that has been systematically reported to exert several therapeutic effects, such as antitumoral, antidiabetic, antioxidant, anti-inflammatory, antiparasitic, and antiviral properties. recently, new derivatives of maslinic acid have been obtained and expanded the spectrum of biological activities and improved the existing ones. the present study was meant to perform the in vitro assessment of the (i) cytotoxic effects of a benzylamid ...201628050335
epidemiology of nosocomial infections: about 70 cases.background - the infections associated with care (ias) constitute a major public health problem by the morbi- mortality than they generate as well as by their cost to support. the prevention of these infections has become a priority for several years everywhere in the world and in the occurrence in tunisia. aim - the purpose of our study is to assess the prevalence of nosocomial infections as well as develop their microbiologic  profiles in the different departments of  the hospital of fsi  mars ...201628051232
emergence of a novel β-lactamase in food of animal origin: characterization of shv-111, from a cheese isolate of klebsiella pneumoniae, with an amino acid substitution in a strictly conserved position (p174s) in the ω-loop. 201728051951
antimicrobial susceptibility of gram-negative eskape pathogens isolated from hospitalized patients with intra-abdominal and urinary tract infections in asia-pacific countries: smart 2013-2015.gram-negative eskape pathogens (klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa and enterobacter spp.) are responsible for increases in antimicrobial-resistant infections worldwide. we determined in vitro susceptibilities to eight parenteral antimicrobial agents using clinical and laboratory standards institute broth microdilution methodology for gram-negative eskape pathogens isolated from hospitalized patients with intra-abdominal infections (iais) (n=3052) and urinary t ...201728051952
detection of ges-5 carbapenemase in klebsiella pneumoniae, a newcomer in france. 201728052846
comparison of phenotypic and pcr methods for detection of carbapenemases production by enterobacteriaceae.dissemination of carbapenem resistance via enterobacteriaceae, particularly among klebsiella pneumoniae and escherichia coli, is a major public health concern. rapid methods for determining antimicrobial susceptibility are important to ensure adequate and appropriate use of antimicrobial agents and to limit the spread of these bacteria. in the current study, we compared the rapidity, sensitivity and specificity of traditional methods and molecular-based xpert carba-r pcr assay to identify sixty ...201728053586
the possible mode of antitussive and expectorant activity of the ethanol seed extracts of picralima nitida ((stapf) th. & h. durand).it has been established that picralima nitida has antitussive effect. this study therefore aimed at determining the possible mode of antitussive and expectorant activity of an ethanolic seed extract of p. nitida (pne). the muco-suppressant, mast cell stabilization, and the anxiolytic effects of pne were ascertained using ammonium chloride-induced phenol red secretion in balb/c mice; compound 48/80-induced mesenteric mast cell degranulation assay; and the open field and the elevated plus maze mod ...201728053900
prevention of evisceration or enucleation in endogenous bacterial panophthalmitis with no light perception and scleral abscess.panophthalmitis is the most extensive ocular involvement in endophthalmitis with inflammation in periocular tissues. severe inflammation of the anterior and posterior segments is frequently accompanied by corneal opacity, scleral abscess, and perforation or rupture. enucleation or evisceration was the only remaining viable treatment option when all options to salvage the eye had been exhausted. the purpose of this retrospective study is to examine the outcomes of patients with endogenous bacteri ...201728056067
antimicrobial effectiveness of synthesized bioactive silver nanoparticles by the actinomycetes hgg16n strain.biologically synthetized silver nanoparticles are promising antimicrobial agent. flow cytometry, well diffusion methods, colony-forming units (cfu) and spectroscopic approach are commonly used in antimicrobial study. the aims of this study were to provides information about effectiveness of bioagnps synthesized by actinomycetes hgg16n using fluorescence flow cytometry method as an alternative to the standard ones (well and disc diffusion method).201728056728
strain-specific transmission in an outbreak of esbl-producing enterobacteriaceae in the hemato-oncology care unit: a cohort study.extended-spectrum β-lactamase (esbl)-producing bacteria are resistant to several types of antibiotics excluding carbapenems. a transmissibility of esbl-producing enterobacteriaceae would be depending on each bacterial property, however, that has not been elucidated in clinical setting. in this study, we attempted to identify the source of an outbreak of esbl-producing bacteria in a medical oncology and immunology care unit.201728056827
epidemiology and control measures of outbreaks due to antibiotic-resistant organisms in europe (embargo): a systematic review protocol.improving our understanding of outbreaks due to antibiotic-resistant bacteria (arb) and their control is critical in the current public health scenario. the threat of outbreaks due to arb requires multifaceted efforts. however, a global overview of epidemiological characteristics of outbreaks due to arb and effective infection control measures is missing. in this paper, we describe the protocol of a systematic review aimed at mapping and characterising the epidemiological aspects of outbreaks du ...201728057656
local and global dynamics in klebsiella pneumoniae outer membrane protein a in lipid bilayers probed at atomic resolution.the role of membrane proteins in cellular mechanism strongly depends on their dynamics, and solid-state magic-angle spinning (mas) nuclear magnetic resonance (nmr) is a unique method to exhaustively characterize motions of proteins in a lipid environment. herein, we make use of advances in (1)h-detected mas nmr to describe the dynamics of the membrane domain of the outer membrane protein a of klebsiella pneumoniae (kpompa). by measuring (1)h-(15)n dipolar-coupling as well as (15)n r1 and r1ρ rel ...201728059506
role of inoculum and mutant frequency on fosfomycin mic discrepancies by agar dilution and broth microdilution methods in enterobacteriaceae.fosfomycin is re-evaluated as a treatment of multidrug-resistant enterobacteriaceae infections. however, mic differences have been described among the different susceptibility testing. the aim was to study the role of the different inoculum size used in agar dilution with respect to broth microdilution, according to clsi, in the fosfomycin mic discrepancies.201728062317
bacterial contamination of platelet products in the blood transfusion center of isfahan, iran.aim: overall the risk of transfusion transmitted infections has decreased, especially viral infections like hiv and hepatitis b and c. bacterial contamination of blood and its cellular components, however, remains a common microbiological cause of transfusion associated morbidity and mortality. platelets pose a special risk given their preservation methods. the incidence of these episodes needs to be assessed and updated on regular basis to accurately manage the risk of transfusion transmitted b ...201628066700
physicochemical and antimicrobial properties of copaiba oil: implications on product quality control.the copaiba oil is a common natural product used in cosmetic industry and as a nutraceutical product. however, lack of quality control and scarce knowledge about its antimicrobial activity is a point of concern. the proposal of this study was to investigate the physicochemical properties and the antimicrobial activity of five commercial brands of copaiba oil.201728068029
antimicrobial activity of ceftazidime-avibactam and comparator agents when tested against bacterial isolates causing infection in cancer patients (2013-2014).we evaluated the antimicrobial susceptibility of 623 gram-negative organisms causing infection in patients with cancer in 52 united states hospitals (2013-2014) as part of the international network for optimal resistance monitoring (inform) program. isolates were tested for susceptibility by broth microdilution method. β-lactamase encoding genes were evaluated for all escherichia coli and klebsiella spp. with an extended-spectrum β-lactamase (esbl) phenotype by microarray-based assay. esbl-pheno ...201728069328
antimicrobial activity of ceftazidime-avibactam against gram-negative bacteria isolated from patients hospitalized with pneumonia in u.s. medical centers, 2011 to 2015.bacterial isolates were collected from patients hospitalized with pneumonia (php), including ventilator-associated pneumonia (vap), from 76 u.s. medical centers in 2011 to 2015. the gram-negative organisms (n = 11,185, including 1,097 from vap) were tested for susceptibility to ceftazidime-avibactam and comparators by the broth microdilution method. β-lactamase-encoding genes were screened using a microarray-based assay on selected isolates. pseudomonas aeruginosa and klebsiella spp. were the mo ...201728069649
[clinical analysis for patients with continuous ambulatory peritoneal dialysis associated peritonitis].to analyze the clinical characteristics of continuous ambulatory peritoneal dialysis (capd) associated peritonitis in the tertiary hospitals and to discuss the preventive and therapeutic strategy.
 methods: the clinical characteristics, pathogens, resistance and outcomes of 126 capd associated peritonitis in 104 patients from jan, 2013 to june, 2016, were retrospectively analyzed.
 results: among the patients, the incidence rates of abdominal pain, fever, diarrhea and emesis were 104 (82.54%), 5 ...201628070047
variable coordination and c-s bond cleavage activity of n-substituted imidazolidine-2-thiones towards copper: synthesis, spectroscopy, structures, esi-mass and antimicrobial studies.an equimolar reaction of copper(i) iodide with n-ethyl-imidazolidine-2-thione (l-et) in acetonitrile formed black prismatic crystals over a period of three weeks. the x-ray structure determination of black crystals revealed that the thio-ligand l-et has transformed into a new thio-ligand, 1-ethyl-3-(1-ethyl-4,5-dihydro-1h-imidazol-2-yl)imidazolidine-2-thione (l-net), through c-s rupture/c-n bond formation which was coordinated in the unusual 2d polymer, {(cu(ii)(κ(2)-n,s-l-net)2)·(cui4)·(cui2)}n ...201728070570
screening of esbl-producing enterobacteriacae concomitant with low degree of transmission in intensive care and bone marrow transplant units.extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-e) are spreading worldwide in both hospital and community settings. in this study, the molecular epidemiology and the transmission modalities of esbl-e in intensive care- and bone marrow transplant were investigated.201728071193
molecular epidemiology and resistome analysis of multidrug-resistant st11 klebsiella pneumoniae strain containing multiple copies of extended-spectrum β-lactamase genes using whole-genome sequencing.the aim of this work was to investigate the mechanism responsible for multidrug resistance in st11 klebsiella pneumoniae ymc 2013/7/b3993 containing multiple copies of esbl genes using multiple parallel sequencing technology. in-depth analysis of the strain revealed multiple copies of esbl genes, 2 copies of blashv-12 and 1 copy of blactx-m-15. furthermore, 1 copy of blaoxa-9 and 3 copies of blatem-1 were found. the insertion of tn1331 was detected, which consisted of blaoxa-9, blatem-1, aac(6') ...201728072891
spread of mcr-1-carrying enterobacteriaceae in sewage water from spain.the mobile colistin resistance gene mcr-1 has been identified worldwide in human and animal sources, while its occurrence in the environment is still largely unknown. the aim of this study was to investigate the presence of mcr-1 -harbouring enterobacteriaceae in water samples obtained from rivers and waste water treatment plants in the area of barcelona, spain.201728073965
characterization of carbapenemase-producing enterobacteriaceae in the west midlands region of england: 2007-14.carbapenemase-producing enterobacteriaceae (cpe) have been increasingly reported in the uk since 2003. we analysed patient and isolate data for cpe confirmed by the national reference laboratory from laboratories in the west midlands region from november 2007 to december 2014.201728073969
the emergence of carbapenem resistant klebsiella pneumoniae in malaysia: correlation between microbiological trends with host characteristics and clinical factors.carbapenem resistant enterobacteriaceae is a growing concern worldwide including malaysia. the emergence of this pathogen is worrying because carbapenem is one of the 'last-line' antibiotics. the main objective of this study was to determine the prevalence of genetic mechanisms and clinical risk factors of carbapenem resistant klebsiella pneumoniae (k. pneumoniae) in malaysia.201728074126
the mito-damp cardiolipin blocks il-10 production causing persistent inflammation during bacterial pneumonia.bacterial pneumonia is a significant healthcare burden worldwide. failure to resolve inflammation after infection precipitates lung injury and an increase in morbidity and mortality. gram-negative bacteria are common in pneumonia and increased levels of the mito-damage-associated molecular pattern (damp) cardiolipin can be detected in the lungs. here we show that mice infected with klebsiella pneumoniae develop lung injury with accumulation of cardiolipin. cardiolipin inhibits resolution of infl ...201728074841
vps13f links bacterial recognition and intracellular killing in dictyostelium.bacterial sensing, ingestion, and killing by phagocytic cells are essential processes to protect the human body from infectious microorganisms. the cellular mechanisms involved in intracellular killing, their relative importance, and their specificity towards different bacteria are however poorly defined. in this study, we used dictyostelium discoideum, a phagocytic cell model amenable to genetic analysis, to identify new gene products involved in intracellular killing. a random genetic screen l ...201728076662
first report of bla ctx-m-15-type esbl-producing klebsiella pneumoniae in wild migratory birds in pakistan.we investigated wild migratory birds faecal swabs for extended-spectrum β-lactamases-producing klebsiella pneumoniae (esbl-k. pneumoniae) from wetland habitats in pakistan. esbl-k. pneumoniae were analysed for mdr phenotype, esbl genotype and genetic diversity. a total of 13 (8.6%) esbl-k. pneumoniae were recovered. of these, 8 (61%) isolates were mdr. dna sequencing confirmed bla ctx-m-15 as the dominant esbl genotype. box-pcr fingerprints showed most of the isolates are unrelated. this study i ...201728078492
aerobic microflora and endoparasites of freshly shot wild agouti (dasyprocta leporina) in trinidad, west indies.the agouti ( dasyprocta leporina ) is a new world wild rodent hunted for its meat in trinidad and other latin american countries. studies on agouti under captive conditions have yielded some data on health-related aspects, but relatively very little is known about their wild counterparts. the environment of the agouti can influence the microflora and parasites harbored by the animals, which may contain zoonotic pathogens. here, the microflora found on the nasal mucosa and sections of the intesti ...201628080916
notes from the field: pan-resistant new delhi metallo-beta-lactamase-producing klebsiella pneumoniae - washoe county, nevada, 2016. 201728081065
differences in inflammatory response induced by two representatives of clades of the pandemic st258 klebsiella pneumoniae clonal lineage producing kpc-type carbapenemases.st258-k. pneumoniae (st258-kp) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 kb genomic region that includes the cluster of capsule genes. to investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of st258-kp clades, kkbo-1 and kk207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts. the two st258-kp ...201728081233
[renal abnormalities]. 201728081902
draft genome sequence of an environmental multidrug-resistant klebsiella pneumoniae st340/cc258 harbouring blactx-m-15 and blakpc-2 genes.anthropogenic activities, including the release of wastewater and sewage from hospitals, have contributed to the contamination of aquatic environments, raising a concern to public health. in this study, we present the first draft genome sequence of a klebsiella pneumoniae strain (kp171, tiet-4200) belonging to the high-risk hospital-associated clonal lineage st340/cc258, which was recovered from a water sample collected in an urban river in brazil.201728082142
etiologies of community-onset urinary tract infections requiring hospitalization and antimicrobial susceptibilities of causative microorganisms.community-onset urinary tract infections (coutis) are the most common bacterial infections, and a decline in antibiotic susceptibility causes many clinical challenges. adequate empiric antibiotic treatment can decrease unnecessary hospital stays and complications, while reducing the antimicrobial resistance progression.201628087318
an observational study of phagocytes and klebsiella pneumoniae relationships: different behaviors.klebsiella pneumoniae is a bacterium that can be in relation with free living amoebae like acanthamoeba castellanii in natural environments such as soil and water. this pathogen, which is responsible for community-acquired pneumonia and for nosocomial infections, also has interactions with host defense mechanisms like macrophages. as it has been shown that a. castellanii shares some traits with macrophages, in particular the ability to phagocyte bacteria, we have studied the uptake and the fate ...201728087454
in vitro activity of ceftazidime/avibactam against urinary isolates from patients in a phase 3 clinical trial programme for the treatment of complicated urinary tract infections.to evaluate the in vitro activity of ceftazidime/avibactam relative to comparator agents against gram-negative isolates from a phase 3 clinical trial programme for complicated urinary tract infections (recapture).201728088768
cathelicidin antimicrobial peptide from alligator mississippiensis has antibacterial activity against multi-drug resistant acinetobacter baumanii and klebsiella pneumoniae.alligator mississippiensis (american alligator), a member of order crocodilia, lives in bacteria-laden environments but is not often known to succumb to bacterial infections. their serum has been shown to have antibacterial activity beyond that of human serum, and it is believed that this activity is partially due to cationic antimicrobial peptides (camps). camps are produced by many organisms as part of the innate immune system. camps are attractive possible therapies against multi-drug resista ...201728089718
antibacterial activity of limonium brasiliense (baicuru) against multidrug-resistant bacteria using a statistical mixture design.limonium brasiliense (boiss.) kuntze (plumbaginaceae) is commonly known as "baicuru" or "guaicuru" and preparations of its dried rhizomes have been popularly used in the treatment of premenstrual syndrome and menstrual disorder, and as an antiseptic in genito-urinary infections. this study evaluated the potential antibacterial activity of rhizome extracts against multidrug-resistant bacterial strains using statistical mixture design.201728089736
phagebiotics in treatment and prophylaxis of healthcare-associated infections.we have developed a phagebiotic composition using 8 virulent bacteriophages (2 strains of each species) which are able to lyse acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and staphylococcus aureus. the unique character of the developed composition is ensured by particular properties of each bacteriophage comprising the preparation, including their range of lytic activity toward specific bacterial pathogens, morphology of their plaques, cycle of their development, restr ...201628090384
fecal carriage of extended-spectrum β-lactamase-producing enterobacteriaceae strains is associated with worse outcome in patients hospitalized in the pediatric oncology unit of beni-messous hospital in algiers, algeria.the current study aimed to investigate extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-e) fecal carriage in children with different cancers admitted in the pediatric oncology unit of beni-messous hospital (algiers, algeria).201728095119
effect of intermittent aeration on microbial diversity in an intermittently aerated ifas reactor treating municipal wastewater: a field study.in the present study, the effect of three intermittent aeration (ia) cycles on treatment performance and microbial diversity was investigated in an integrated fixed film activated sludge (ifas) reactor treating municipal wastewater. the results showed that ia strategies were able to achieve efficient removal of organics and nitrogen ranging between 90 and 95% and 70 and 80%, respectively, however the phosphorus removal was found to be inversely proportional to the duration of aeration off time i ...201728095182
multi-institute analysis of carbapenem resistance reveals remarkable diversity, unexplained mechanisms, and limited clonal outbreaks.carbapenem-resistant enterobacteriaceae (cre) are among the most severe threats to the antibiotic era. multiple different species can exhibit resistance due to many different mechanisms, and many different mobile elements are capable of transferring resistance between lineages. we prospectively sampled cre from hospitalized patients from three boston-area hospitals, together with a collection of cre from a single california hospital, to define the frequency and characteristics of outbreaks and d ...201728096418
meropenem dosing based on a population pharmacokinetic-pharmacodynamic model in elderly patients with infection of the lower respiratory tract.meropenem is used for the treatment of severe lower respiratory tract infections (lrtis) caused by multidrug-resistant gram-negative bacilli.201728097633
emergence of blandm-7-producing enterobacteriaceae in gabon, 2016.reports of carbapenemase-producing enterobacteriaceae in africa remain rare and assess mostly blaoxa-48-producing isolates from mediterranean countries and south africa. we identified blandm-7-producing enterobacteriaceae in gabon in 2016. the isolates contained blandm-7 incx3 plasmids that were unusual and similar to the one described in a colistin-resistant klebsiella pneumoniae sz04 isolate from china.201728098536
rectal carriage of extended-spectrum beta-lactamase and carbapenemase producing gram-negative bacilli in intensive care units in tunisia.this study was conducted to evaluate the rate of fecal carriage of gram-negative bacilli (gnb) resistant to third-generation cephalosporins (third gc) in patients hospitalized in the intensive care unit (icu) of charles nicolle hospital of tunis and to identify the enzymatic mechanisms involved. from february to april 2014, rectal swabs were collected from all patients (n = 38) at admission and once weekly thereafter to identify acquisition. they were cultured on desoxycholate-lactose-agar plate ...201728099062
clinical and microbiological profile of babies born with risk of neonatal sepsis.the aim of the present study was to evaluate the effects of antibiotics on the condition of babies born with risk of neonatal sepsis. from march, 2014 to february, 2015, 200 neonates born with risk factors of septicemia in the neonatal intensive care unit at xuzhou central hospital, were enrolled in the present study. venous blood samples were collected within 6 h of birth using aseptic technique. part of the blood specimens were cultured using bactec peds plus/f culture vials. subsequently, the ...201628101156
carbapenemase-producing klebsiella pneumoniae bloodstream infection in critically ill patients: risk factors and predictors of mortality.a significant increase in carbapenemase-producing klebsiella pneumoniae (cp-kp) bacteraemias has been observed worldwide. the objective of the present work was to study the risk factors and predictors of mortality of cp-kp bacteraemias among critically ill patients. during a 4-year period (2012-3015), a matched 1:2 case-control study was conducted. klebsiella pneumoniae was identified by vitek 2 technology. antibiotic susceptibility was performed by the agar disc diffusion method and etest. the ...201728102514
enhancement of antibody functions through fc multiplications.antibodies carry out a plethora of functions through their crystallizable fragment (fc) regions, which can be naturally tuned by the adoption of several isotypes and post-translational modifications. protein engineering enables further fc function modulations through modifications of the interactions between the fc and its functional partners, including fcγr, fcrn, complement complex, and additions of auxiliary functional units. due to the many functions embedded within the confinement of an fc, ...201728102754
endotoxin neutralization by an o-antigen specific monoclonal antibody: a potential novel therapeutic approach against klebsiella pneumoniae st258.klebsiella pneumoniae st258 is a globally distributed multi-drug resistant pathogen responsible for severe invasive infections. in this study, the different virulence potential of k. pneumoniae st258 isolates in endotoxin susceptible versus resistant animal models was shown. furthermore, st258 clinical isolates were found highly sensitive to the bactericidal effect of naive animal and human serum. these observations imply that lps, released from the rapidly lysed bacteria, may contribute to the ...201728103139
clinical study on cluster care to prevent multi-drug resistant infection in icu patients with severe encephalopathy.the value of clinical cluster nursing in the prevention of multi-drug resistant (mdr) infection in patients with severe encephalopathy in icu was evaluated. icu patients (n=129) diagnosed with severe encephalopathy between 2012 and 2014 were selected as the study group, while 106 cases of icu patients diagnosed with severe encephalopathy between 2010 and 2012 were retrospectively selected as the control group. control group patients were offered conventional integrated nursing care, while the st ...201628105127
acinetodin and klebsidin, rna polymerase targeting lasso peptides produced by human isolates of acinetobacter gyllenbergii and klebsiella pneumoniae.we report the bioinformatic prediction and structural validation of two lasso peptides, acinetodin and klebsidin, encoded by the genomes of several human-associated strains of acinetobacter and klebsiella. computation of the three-dimensional structures of these peptides using nmr noesy constraints verifies that they contain a lasso motif. despite the lack of sequence similarity to each other or to microcin j25, a prototypical lasso peptide and transcription inhibitor from escherichia coli, acin ...201728106375
anti-mrka monoclonal antibodies reveal distinct structural and antigenic features of mrka.antibody therapy against antibiotics resistant klebsiella pneumoniae infections represents a promising strategy, the success of which depends critically on the ability to identify appropriate antibody targets. using a target-agnostic strategy, we recently discovered mrka as a potential antibody target and vaccine antigen. interestingly, the anti-mrka monoclonal antibodies isolated through phage display and hybridoma platforms all recognize an overlapping epitope, which opens up important questio ...201728107434
prostatic abscess of klebsiella pneumonia complicating septic pulmonary emboli and meningitis: a case report and brief review.prostatic abscess is a rare entity with an incidence of 0.5%-2.5% in all prostate diseases and usually occurs in the 5th and 6th decades of life with immunocompromised status. prostatic abscess might be a process of evolution from acute prostatitis. klebsiella pneumoniae is the leading microorganism in the diabetic patients of prostatic abscess in taiwan. a 60-year-old diabetic man, with a one-week history of acute bacterial prostatitis was reported in this study, presenting to the emergency dep ...201728107857
in vitro susceptibility to 19 agents other than β-lactams among third-generation cephalosporin-resistant enterobacteriaceae recovered on hospital admission.as part of the multicentre antibiotic therapy optimisation study, mic values of 19 non-β-lactam agents were determined for third-generation cephalosporin-resistant escherichia coli, klebsiella species and enterobacter species (3gcreb) isolates collected in german hospitals.201728108677
clinical and microbiological characteristics of bloodstream infections among patients with haematological malignancies with and without neutropenia at a medical centre in northern taiwan, 2008-2013.this study aimed to investigate the epidemiology and microbiology of bloodstream infections (bsis) in patients with haematological malignancies. clinical characteristics and microbiology of 2083 patients with haematological malignancy who were treated for bsi from 2008 to 2013 at a medical centre in taiwan were retrospectively reviewed. lymphoma (38.1%) was the most common, followed by acute myeloid leukaemia (30.9%). of the 2090 non-duplicate bsi isolates, 1310 (62.7%) were recovered from patie ...201728109554
synthesis and in vitro activity of dicationic indolyl diphenyl ethers as novel potent antibiotic agents against drug-resistant bacteria.a series of 4,4'-bis-[2-(6-n-substituted-amidino)indolyl] diphenyl ether have been synthesized and tested for their in vitro antibacterial activity including a range of gram-positive and gram-negative pathogens and cytotoxicity. most of these compounds have mainly shown anti-gram positive bacteria activities especially against drug resistant bacterial strains mrsa, mrse and vre. the anti-mrsa and anti-mrse activities of compound 7a and 7j were more potent than that of the lead compound 2, levofl ...201728109787
outbreak of nosocomial ndm-1-producing klebsiella pneumoniae st1419 in a neonatal unit.the aim of this study was to characterise carbapenem-resistant klebsiella pneumoniae isolates recovered from neonatal clinical specimens over a 4-month period.201728109845
molecular characterisations of integrons in clinical isolates of klebsiella pneumoniae in a chinese tertiary hospital.integrons are mobile genetic elements that play an important role in the distribution of antibiotic-resistance genes among bacteria. this study aimed to investigate the distribution of integrons in clinical isolates of klebsiella pneumoniae and explore the molecular mechanism of integron-mediated multiple-drug resistance in k. pneumoniae.201728111326
[clonal relationship and detection of blakpc gene in strains of klebsiella pneumoniae resistant to carbapenems, at a hospital in venezuela].in order to study the clonal relationship and blakpc gene detection in clinical isolates of klebsiella pneumoniae resistant to carbapenems, we analyzed 22 clinical isolates of k. pneumoniae with resistance to imipenem and/ or meropenem, isolated in the laboratory of bacteriology at the university hospital "antonio patricio de alcalá" (huapa) from the cumana city, sucre state, venezuela, for a period of five consecutive years. susceptibility to different antimicrobials was determined, and the pre ...201628112335
coexistence of oxa-48-producing klebsiella pneumoniae and escherichia coli in a hospitalized patient who returned from europe to china. 201728115343
survival of carbapenem-resistant klebsiella pneumoniae sequence type 258 in human blood.klebsiella pneumoniae is a prominent cause of nosocomial infections worldwide. bloodstream infections caused by carbapenem-resistant k. pneumoniae, including the epidemic lineage known as multilocus sequence type 258 (st258), are difficult to treat, and the rate of mortality from such infections is high. thus, it is imperative that we gain a better understanding of host defense against this pathogen as a step toward developing novel therapies. here we tested the hypothesis that the resistance of ...201728115349
biological treatment of cyanide by using
klebsiella pneumoniae species.in this study, optimization conditions for cyanide biodegradation by klebsiella pneumoniae strain were determined to be 25 °c, ph=7 and 150 rpm at the concentration of 0.5 mm potassium cyanide in the medium. additionally, it was found that k. pneumoniae strain is not only able to degrade potassium cyanide, but also to degrade potassium hexacyanoferrate(ii) trihydrate and sodium ferrocyanide decahydrate with the efficiencies of 85 and 87.5%, respectively. furthermore, this strain degraded potassi ...201628115902
a label-free electrochemical biosensor based on a reduced graphene oxide and indole-5-carboxylic acid nanocomposite for the detection of klebsiella pneumoniae.a label-free dna hybridization electrochemical sensor for the detection of klebsiella pneumoniae was developed, which could be helpful in the diagnosis of bacterial infections. indole-5-carboxylic acid (ica) and graphene oxide (go) were electrodeposited on a glassy carbon electrode, and the resulting reduced go (rgo)-ica hybrid film served as a platform for immobilizing oligonucleotides on a single-stranded dna (ssdna) sequence. the conditions were optimized, with excellent electrochemical perfo ...201728118564
whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental klebsiella pneumoniae.klebsiella pneumoniae is a gram-negative bacterial species capable of occupying a broad range of environmental and clinical habitats. known as an opportunistic pathogen, it has recently become a major causative agent of clinical infections worldwide. despite growing knowledge about the highly diverse population of k. pneumoniae, the evolution and clinical significance of environmental k. pneumoniae, as well as the relationship between clinical and environmental k. pneumoniae, are poorly defined.201728118859
a comparison of antibacterial and antibiofilm efficacy of phenothiazinium dyes between gram positive and gram negative bacterial biofilm.antimicrobial photodynamic therapy (apdt) is a process that generates reactive oxygen species (ros) in presence of photosensitizer, visible light and oxygen which destroys the bacterial cells. we investigated the photoinactivation efficiency of phenothiazinium dyes and the effect of ros generation on gram positive and gram negative bacterial cell as well as on biofilm.201728119141
diversity and functionality of plasmid-borne vagcd toxin-antitoxin systems of klebsiella pneumoniae.to explore the vagcd toxin-antitoxin (ta) systems encoded on plasmids in multiresistant klebsiella pneumoniae strains.201728119479
molecular mechanisms of colistin resistance in klebsiella pneumoniae causing bacteremia from india-a first report.colistin has long been a reserve drug used for the treatment of carbapenem resistant klebsiella pneumoniae. carbapenem resistance in k. pneumoniae has been increasing and is as high as 44% in india. although a reserve agent, with rise in rates of resistance to carbapenems, the usage of colistin has increased over the years leading to slow emergence of resistance. colistin resistance is mainly mediated by the alteration in the lps of bacterial outer membrane with the addition of l-ara4-n and petn ...201628119670
saccharothrix sp. td-093 from the thar desert, india: metabolite fingerprinting of antimicrobial compounds and in silico analysis.during a screening program for actinomycetes from underexplored and arid thar desert (india), td-093 was isolated. the isolate was characterized based on 16s rdna sequencing. aqueous and organic solvent extracts of culture supernatant were investigated for antimicrobial activity. bioactive fractions, after column chromatography separation, were subjected to gc-ms analysis. based on 16s rdna sequence result, isolate td-093 showed nearest match to saccharothrix (96%) and is a potential new species ...201728120024
epidemiology and susceptibility of pathogens from smart 2011-12 turkey: evaluation of hospital-acquired versus community-acquired urinary tract infections and icu- versus non-icu-associated intra-abdominal infections.to describe the epidemiology and susceptibility of pathogens (including esbl producers) from hospital-acquired (ha) versus community-acquired (ca) urinary tract infections (utis) and icu- versus non-icu-associated intra-abdominal infections (iais) in turkey as a part of the smart study.201728122913
global assessment of the activity of tigecycline against multidrug-resistant gram-negative pathogens between 2004 and 2014 as part of the tigecycline evaluation and surveillance trial.multidrug-resistant (mdr) gram-negative organisms are a burden on the global health care system. the tigecycline evaluation and surveillance trial (test) is an ongoing global study designed to monitor the in vitro activities of tigecycline and a panel of marketed antimicrobials against a range of clinically significant pathogens. in this study, in vitro data are presented for mdr acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterob ...201728124025
participation of necroptosis in the host response to acute bacterial pneumonia.common pulmonary pathogens, such as streptococcus pneumoniae and staphylococcus aureus, as well as the host-adapted pathogens responsible for health care-associated pneumonias, such as the carbapenem-resistant klebsiella pneumoniae and serratia marcecsens, are able to activate cell death through the ripk1/ripk3/mlkl cascade that causes necroptosis. necroptosis can influence the pathogenesis of pneumonia through several mechanisms. activation of this pathway can result in the loss of specific typ ...201728125817
bacteremia and other body site infection caused by hypervirulent and classic klebsiella pneumoniae.to investigate bacteremia and other body site infection caused by hypervirulent klebsiella pneumoniae (hvkp), a recently recognized pathogen of invasive infection, and classic klebsiella pneumoniae (ckp), a very common organism associated with many kinds of nosocomial infection.201728132768
panel strain of klebsiella pneumoniae for beta-lactam antibiotic evaluation: their phenotypic and genotypic characterization.klebsiella pneumoniae is responsible for numerous infections caused in hospitals, leading to mortality and morbidity. it has been evolving as a multi-drug resistant pathogen, acquiring multiple resistances such as such as horizontal gene transfer, transposon-mediated insertions or change in outer membrane permeability. therefore, constant efforts are being carried out to control the infections using various antibiotic therapies. considering the severity of the acquired resistance, we developed a ...201728133574
challenges and opportunities for whole-genome sequencing-based surveillance of antibiotic resistance.infections caused by drug-resistant bacteria are increasingly reported across the planet, and drug-resistant bacteria are recognized to be a major threat to public health and modern medicine. in this review, we discuss how whole-genome sequencing (wgs)-based approaches can contribute to the surveillance of the emergence and spread of antibiotic resistance. we outline the characteristics of sequencing technologies that are currently most used for wgs (illumina short-read technologies and the long ...201728134443
the essential oil from the fruits of the brazilian spice xylopia sericea a. st.-hil. presents expressive in-vitro antibacterial and antioxidant activity.the aims of this study were to investigate the chemical composition and the antioxidant activity and antibacterial activity of the essential oil of xylopia sericea fruits (oxs). the fruits of this species are popularly used for medicinal purposes, and as a condiment in food preparation.201728134988
use of colistin in a neonatal intensive care unit: a cohort study of 65 patients.background the emergence of infections related to multidrug-resistant gram-negative bacilli (mdr-gnb) reintroduced the use of colistin, an antibiotic that was previously abandoned due to adverse effects. however, because of its limited use in neonatal intensive care units, there is very little data about the effectiveness and safety of colistin in children and newborns. in this study, which will be the largest case study in the literature, we aimed to evaluate the effectiveness and safety of col ...201728135233
antibodies directed against a peptide epitope of a klebsiella pneumoniae-derived protein are present in ankylosing spondylitis.ankylosing spondylitis (as) is a chronic inflammatory arthritis of unknown origin. its autoimmune origin has been suggested but never proven. several reports have implicated klebsiella pneumoniae as a triggering or perpetuating factor in as; however, its role in the disease pathogenesis remains debated. moreover, despite extensive investigations, a biomarker for as has not yet been identified. to clarify these issues, we screened a random peptide library with pooled iggs obtained from 40 patient ...201728135336
spread of esbl/ampc-producing escherichia coli and klebsiella pneumoniae in the community through ready-to-eat sandwiches in algeria.the spread of extended-spectrum β-lactamases (esbls) or ampc β-lactamases (ampc) encoding genes in healthy human populations is of major concern. the role of the food chain has been questioned since numerous studies reported surface contamination of retail meat or crude vegetables with esbl/ampc-producing enterobacteriaceae (esbl/ampc-e). nonetheless, these food products are intended to be cooked or washed before consumption so that the risk of human transfer might be low. here, the presence of ...201728135647
antimicrobial activity of high-proportion cefepime-tazobactam (wck 4282) against a large number of gram-negative isolates collected worldwide in 2014.cefepime-tazobactam (wck 4282) is currently under clinical development for use at a dosage of 2 g/2 g every 8 h. a total of 7,981 isolates were collected from 146 medical centers (39 countries) in 2014 as a part of the sentry antimicrobial surveillance program, and their susceptibilities to cefepime-tazobactam (with tazobactam at fixed concentrations of 4 and 8 μg/ml) were tested by a reference broth microdilution method. isolates were mainly from patients with pneumonia (29.5%) and bloodstream ...201728137811
the carbapenemase-producing klebsiella pneumoniae population is distinct and more clonal than the carbapenem-susceptible population.we studied in parallel the population structure of 90 carbapenemase-producing and 88 carbapenemase-susceptible klebsiella pneumoniae isolates collected in 20 spanish hospitals, in the context of the euscape project. fourteen and 50 multilocus sequence types (mlsts) were detected among the carbapenemase-producing and carbapenem-susceptible isolates, respectively. st11 and st15 clones were more frequent in the carbapenemase-producing group than in the carbapenemase-susceptible group (p < 0.0001). ...201728137818
antimicrobial resistance in uropathogens and appropriateness of empirical treatment: a population-based surveillance study in indonesia.urinary tract infections (utis) are a common reason for empirical treatment with broad-spectrum antibiotics worldwide. however, population-based antimicrobial resistance (amr) prevalence data to inform empirical treatment choice are lacking in many regions, because of limited surveillance capacity. we aimed to assess the prevalence of amr to commonly used antimicrobial drugs in escherichia coli and klebsiella pneumoniae isolated from patients with community- or healthcare-associated utis on two ...201728137940
preferential pattern of mouse neutrophil cell death in response to various stimulants.neutrophils undergo cell death processes once their physiological function has been fulfilled. apoptosis, necrosis, pyroptosis, or netosis, a unique form of cell death, could occur, depending on the type of stimulant or inhibitory intervention. we investigated whether phorbol myristate acetate (pma) and klebsiella pneumoniae (kp), serving as stimulants, or whether an inhibitor (cytochalasin b, cytb) could alter the morphology and gene expression pattern associated with mouse neutrophil cell deat ...201728138860
nosocomial transmission of carbapenem-resistant klebsiella pneumoniae elucidated by single-nucleotide variation analysis: a case investigation.identifying transmission route of antimicrobial-resistant pathogen is essential for appropriate infection control strategy in healthcare facilities. we report the utility of single-nucleotide variation analysis in tracing nosocomial transmission of antimicrobial-resistant pathogens by describing a pseudo-outbreak case of carbapenem-resistant klebsiella pneumoniae. the present case highlights that infection control strategy should encompass pathological dissection rooms, neglected but potentially ...201728138943
a one-step multiplex pcr to identify klebsiella pneumoniae, klebsiella variicola, and klebsiella quasipneumoniae in the clinical routine.klebsiella pneumoniae, klebsiella variicola and klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. we propose a multiplex pcr for blashv, blalen and blaokp and their flanking gene (deor). since this scheme focuses only on chromosomal genes, it will be feasible for klebsiella identification in the clinical routine.201728139276
prevalence of mcr-1 in escherichia coli and klebsiella pneumoniae recovered from bloodstream infections in china: a multicentre longitudinal study.polymyxin antibiotics are used as last-resort therapies to treat infections caused by multidrug-resistant gram-negative bacteria. the plasmid-mediated colistin resistance determinant mcr-1 has been identified in enterobacteriaceae in china. we did this study to investigate the prevalence of the mcr-1 gene in clinical isolates from patients with bloodstream infections in china.201728139430
prevalence, risk factors, outcomes, and molecular epidemiology of mcr-1-positive enterobacteriaceae in patients and healthy adults from china: an epidemiological and clinical study.the mcr-1 gene confers transferable colistin resistance. mcr-1-positive enterobacteriaceae (mcrpe) have attracted substantial medical, media, and political attention; however, so far studies have not addressed their clinical impact. herein, we report the prevalence of mcrpe in human infections and carriage, clinical associations of mcr-1-positive escherichia coli (mcrpec) infection, and risk factors for mcrpec carriage.201728139431
an audit of colistin use in neonatal sepsis from a tertiary care centre of a resource-limited country.sepsis due to multidrug-resistant gram-negative pathogens is a challenge for clinicians and microbiologists and has led to use of parenteral colistin. there is a paucity of data regarding safety and efficacy of intravenous colistin use in neonates. the objective of this retrospective analysis was to study the efficacy and safety of intravenous colistin in the treatment of neonatal sepsis.201628139542
pathogenic bacteria distributions and drug resistance analysis in 96 cases of neonatal sepsis.this study aimed to summarize common pathogens and associated drug resistance in neonatal sepsis (ns).201728143490
clonal or not clonal? investigating hospital outbreaks of kpc-producing klebsiella pneumoniae with whole-genome sequencing.whole-genome sequencing (wgs) is a promising tool for identifying transmission pathways in outbreaks caused by multidrug-resistant bacteria. however, it is uncertain how the data produced by wgs can be best integrated into epidemiologic investigations.201728143787
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