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antibiotic resistance in burkholderia species.the genus burkholderia comprises metabolically diverse and adaptable gram-negative bacteria, which thrive in often adversarial environments. a few members of the genus are prominent opportunistic pathogens. these include burkholderia mallei and burkholderia pseudomallei of the b. pseudomallei complex, which cause glanders and melioidosis, respectively. burkholderia cenocepacia, burkholderia multivorans, and burkholderia vietnamiensis belong to the burkholderia cepacia complex and affect mostly c ...201627620956
occurrence and characterization of blandm-5-positive klebsiella pneumoniae isolates from dairy cows in jiangsu, china.to investigate the epidemiological characteristics and the surrounding genetic structure of the blandm-5 gene in klebsiella pneumoniae derived from dairy cows in jiangsu province, china.201727621177
blaoxa-48 carrying clonal colistin resistant-carbapenem resistant klebsiella pneumoniae in neonate intensive care unit, india.bacteria resistant to colistin, a last resort antibiotic reflect the pre-antibiotic era. in this study, colistin resistance carbapenem-resistant k. pneumoniae (col(r)- crkp) strains from neonate's intensive care unit were evaluated. molecular analysis showed that all the four colistin resistant k. pneumoniae isolates were clonally related with strong biofilm formation ability and harbored blashv-34 and blaoxa-48 genes. our result suggested the need of proper surveillance and adequate infection c ...201627622347
4,5-disubstituted 6-aryloxy-1,3-dihydrobenzo[c][1,2]oxaboroles are broad-spectrum serine β-lactamase inhibitors.bacterially expressed β-lactamases are rapidly eroding the clinical utility of the important β-lactam class of antibacterials, significantly impairing our ability to fight serious bacterial infections. this paper describes a study of oxaborole-derived β-lactamase inhibitors in which crystal structures and computational modeling aided in the rational design of analogues with improved spectrum of activity against class a, c, and d enzymes. crystal structures of two of these inhibitors covalently b ...201527622821
lysine-based small molecules that disrupt biofilms and kill both actively growing planktonic and nondividing stationary phase bacteria.the emergence of bacterial resistance is a major threat to global health. alongside this issue, formation of bacterial biofilms is another cause of concern because most antibiotics are ineffective against these recalcitrant microbial communities. ideal future antibacterial therapeutics should possess both antibacterial and anti-biofilm activities. in this study we engineered lysine-based small molecules, which showed not only commendable broad-spectrum antibacterial activity but also potent biof ...201527623313
zinc chelation by a small-molecule adjuvant potentiates meropenem activity in vivo against ndm-1-producing klebsiella pneumoniae.the widespread emergence of antibiotic drug resistance has resulted in a worldwide healthcare crisis. in particular, the extensive use of β-lactams, a highly effective class of antibiotics, has been a driver for pervasive β-lactam resistance. among the most important resistance determinants are the metallo-β-lactamases (mbl), which are zinc-requiring enzymes that inactivate nearly all classes of β-lactams, including the last-resort carbapenem antibiotics. the urgent need for new compounds target ...201527623408
neonatal septicaemia, bacterial isolates and antibiogram sensitivity in maiduguri north-eastern nigeria.the aim and objective of the study was to determine the incidence, bacterial isolates and the antibiogram sensitivity of the isolates in neonates with septicaemia.201627623727
klebsiella pneumoniae siderophores induce inflammation, bacterial dissemination, and hif-1α stabilization during pneumonia.klebsiella pneumoniae is a gram-negative pathogen responsible for a wide range of infections, including pneumonia and bacteremia, and is rapidly acquiring antibiotic resistance. k. pneumoniae requires secretion of siderophores, low-molecular-weight, high-affinity iron chelators, for bacterial replication and full virulence. the specific combination of siderophores secreted by k. pneumoniae during infection can impact tissue localization, systemic dissemination, and host survival. however, the ef ...201627624128
high prevalence of esbl-producing escherichia coli and klebsiella pneumoniae in community-onset bloodstream infections in china.community-onset bloodstream infections (cobsis) caused by esbl-producing escherichia coli (esbl-ec) and esbl-producing klebsiella pneumoniae (esbl-kp) are increasing globally. this study aimed to investigate the epidemiology and risk factors of esbl-ec and esbl-kp in cobsis in china.201727624571
polymyxin monotherapy or in combination against carbapenem-resistant bacteria: systematic review and meta-analysis.the objective of this study was to summarize available data on polymyxin-based combination therapy or monotherapy for carbapenem-resistant gram-negative bacteria.201727624572
an irish outbreak of new delhi metallo-β-lactamase (ndm)-1 carbapenemase-producing enterobacteriaceae: increasing but unrecognized prevalence.carbapenemase-producing enterobacteriaceae (cpe) may cause healthcare-associated infections with high mortality rates. new delhi metallo-β-lactamase-1 (ndm-1) is among the most recently discovered carbapenemases.201627624807
clinical outcomes, drug toxicity, and emergence of ceftazidime-avibactam resistance among patients treated for carbapenem-resistant enterobacteriaceae infections.thirty-seven carbapenem-resistant enterobacteriaceae (cre)-infected patients were treated with ceftazidime-avibactam. clinical success and survival rates at 30 days were 59% (22/37) and 76% (28/37), respectively. in 23% (5/22) of clinical successes, cre infections recurred within 90 days. microbiologic failure rate was 27% (10/37). ceftazidime-avibactam resistance was detected in 30% (3/10) of microbiologic failures.201627624958
glycopeptide antibiotic to overcome the intrinsic resistance of gram-negative bacteria.the emergence of drug resistance along with a declining pipeline of clinically useful antibiotics has made it vital to develop more effective antimicrobial therapeutics, particularly against difficult-to-treat gram-negative pathogens (gnps). many antibacterial agents, including glycopeptide antibiotics such as vancomycin, are inherently inactive toward gnps because of their inability to cross the outer membrane of these pathogens. here, we demonstrate, for the first time, lipophilic cationic (pe ...201627624964
crystal structures of trimethoprim-resistant dfra1 rationalize potent inhibition by propargyl-linked antifolates.multidrug-resistant enterobacteriaceae, notably escherichia coli and klebsiella pneumoniae, have become major health concerns worldwide. resistance to effective therapeutics is often carried by class i and ii integrons that can confer insensitivity to carbapenems, extended spectrum β-lactamases, the antifolate trimethoprim, fluoroquinolones, and aminoglycosides. specifically of interest to the study here, a prevalent gene (dfra1) coding for an insensitive dihydrofolate reductase (dhfr) confers 1 ...201627624966
type 3 fimbriae encoded on plasmids are expressed from a unique promoter without affecting host motility, facilitating an exceptional phenotype that enhances conjugal plasmid transfer.horizontal gene transfer (hgt), the transmission of genetic material to a recipient that is not the progeny of the donor, is fundamental in bacterial evolution. hgt is often mediated by mobile genetic elements such as conjugative plasmids, which may be in conflict with the chromosomal elements of the genome because they are independent replicons that may petition their own evolutionary strategy. here we study differences between type 3 fimbriae encoded on wild type plasmids and in chromosomes. u ...201627627107
klebsiella pneumonia liver abscess syndrome: case presentation to a college student health clinic.the authors describe a case of klebsiella pneumoniae liver abscess (kpla) in a student presenting to a university student health center. the authors also provide a review of kpla and invasive klebsiella pneumoniae liver abscess syndrome (ikplas), including epidemiology, common clinical manifestations, standard diagnostic work-up, management options, and potential complications.201727628340
production of xylonic acid by klebsiella pneumoniae.the glucose oxidation pathway is important for glucose catabolism in klebsiella pneumoniae. gluconic acid and 2-ketogluconic acid are intermediates of this pathway, and the production of these two chemicals has been developed in k. pneumoniae mutants. catalysis characteristic research in this study has shown that xylose is a suitable substrate of the glucose dehydrogenase of this pathway. here, using xylose as substrate, xylonic acid was accumulated in the broth of k. pneumoniae culture, and thi ...201627629123
whole-genome multilocus sequence typing of extended-spectrum-beta-lactamase-producing enterobacteriaceae.molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. whole-genome multilocus sequence typing (wgmlst) has emerged as a whole-genome sequencing (wgs)-based gene-by-gene typing method that may overcome these limitations and has been applied successfully for severa ...201627629900
microbiology of non-tunnelled catheter-related infections.aerobic bacterial infections often complicate vascular access in patients receiving haemodialysis, leading to catheter-related blood stream infections (crbsi). various studies report gram - positive bacteria, staphylococcus aureus (s. aureus) in particular, as the most common aetiologic agent. studies on microbiological analysis in this subset of population from india are very few.201627630843
crp-cyclic amp regulates the expression of type 3 fimbriae via cyclic di-gmp in klebsiella pneumoniae.klebsiella pneumoniae is the predominant pathogen isolated from liver abscesses of diabetic patients in asian countries. however, the effects of elevated blood glucose levels on the virulence of this pathogen remain largely unknown. type 3 fimbriae, encoded by the mrkabcdf genes, are important virulence factors in k. pneumoniae pathogenesis. in this study, the effects of exogenous glucose and the intracellular cyclic amp (camp) signaling pathway on type 3 fimbriae expression regulation were inve ...201627631471
increasing incidence of carbapenemase-producing escherichia coli and klebsiella pneumoniae in belgian hospitals.carbapenemase-producing enterobacteriaceae are increasingly reported worldwide. the aim of the study was to determine the incidence and molecular epidemiology of carbapenemase-producing (cp) escherichia coli and klebsiella pneumoniae (cp-e/k) in belgium. eleven hospital-based laboratories collected carbapenem non-susceptible (cns) isolates of e. coli and k. pneumoniae detected in clinical specimens from january 2013 to december 2014. all cns strains were tested for carbapenemase production and t ...201727639859
holothurians antifungal and antibacterial activity to human pathogens in the persian gulf.pharmaceutical industries now accept that oceans contain a vast array of organisms with unique biological properties. as investigation of antimicrobial activity, three species of holothuria sea cucumbers (holothuria scabra, holothuria parva and holothuria leucospilota) from the persian gulf, iran, were obtained, their different organs: gonads (g), body wall (bw), intestine tract (it), respiratory tree (rt) were isolated and extracted with organic solvents: ethyl acetate and methanol. antifungal ...201727641487
dissemination of imp-4-encoding pimp-hz1-related plasmids among klebsiella pneumoniae and pseudomonas aeruginosa in a chinese teaching hospital.a total of 26 blaimp-4-carrying strains of pseudomonas aeruginosa and klebsiella pneumoniae were isolated from 2009 to 2013 in a chinese teaching hospital, and these strains can be assigned into multiple sequence types or allelic profiles as determined by multilocus sequence typing. of these strains, p. aeruginosa p378 and k. pneumoniae 1220 harbor the imp-4-encoding plasmids pp378-imp and p1220-imp, respectively, whose complete nucleotide sequences are determined to be genetically closely relat ...201627641711
role of interspecies interactions in dual-species biofilms developed in vitro by uropathogens isolated from polymicrobial urinary catheter-associated bacteriuria.most catheter-associated urinary tract infections are polymicrobial. here, uropathogen interactions in dual-species biofilms were studied. the dual-species associations selected based on their prevalence in clinical settings were klebsiella pneumoniae-escherichia coli, e. coli-enterococcus faecalis, k. pneumoniae-e. faecalis, and k. pneumoniae-proteus mirabilis. all species developed single-species biofilms in artificial urine. the ability of k. pneumoniae to form biofilms was not affected by e. ...201627642801
outbreak of oxa-48-producing klebsiella pneumoniae involving a sequence type 101 clone in batna university hospital, algeria.seven nonredundant ertapenem-resistant klebsiella pneumoniae isolates were collected between may 2014 and 19 january 2015 in the nephrology and hematology units of batna university hospital in algeria. all strains coproduced the blaoxa-48, blactx-m-15, blashv-1, and blatem-1d genes. six of these isolates belonged to the pandemic clone sequence type 101 (st101). the blaoxa-48 gene was located on a conjugative incl/m-type plasmid. this is the first known outbreak of oxa-48-producing k. pneumoniae ...201627645236
klebsiella pneumoniae major porins ompk35 and ompk36 allow more efficient diffusion of β-lactams than their escherichia coli homologs ompf and ompc.klebsiella pneumoniae, one of the most important nosocomial pathogens, is becoming a major problem in health care because of its resistance to multiple antibiotics, including cephalosporins of the latest generation and, more recently, even carbapenems. this is largely due to the spread of plasmid-encoded extended-spectrum β-lactamases. however, antimicrobial agents must first penetrate the outer membrane barrier in order to reach their targets, and hydrophilic and charged β-lactams presumably di ...201627645385
[analysis of the pathogenic characteristics of 162 severely burned patients with bloodstream infection].to analyze the distribution and drug resistance of pathogen isolated from severely burned patients with bloodstream infection, so as to provide reference for the clinical treatment of these patients.201627647068
polymyxin b resistance in carbapenem-resistant klebsiella pneumoniae, são paulo, brazil. 201627648951
optimization of the production of extracellular polysaccharide from the shiitake medicinal mushroom lentinus edodes (agaricomycetes) using mutation and a genetic algorithm-coupled artificial neural network (ga-ann).exopolysaccharide (eps) production by a strain of lentinus edodes was studied via the effects of treatments with ultraviolet (uv) irradiation and acridine orange. furthermore, optimization of eps production was studied using a genetic algorithm coupled with an artificial neural network in submerged fermentation. exposure to irradiation and acridine orange resulted in improved eps production (2.783 and 5.548 g/l, respectively) when compared with the wild strain (1.044 g/l), whereas optimization l ...201627649726
impact of bacterial load on pharmacodynamics and susceptibility breakpoints for tigecycline and klebsiella pneumoniae.in the absence of other therapeutic options, tigecycline is used to treat bloodstream infections and pneumonia caused by carbapenemase-producing klebsiella pneumoniae (cp-kp). in this study, the standard and high tigecycline dosing regimens were simulated and tested against different inocula of cp-kp isolates in an in vitro pharmacokinetic (pk)/pharmacodynamic (pd) model.201727650184
intestinal carriage of multidrug-resistant gram-negative bacteria in preterm-infants during hospitalization in neonatal intensive care unit (nicu).the prevalence and antibiotic susceptibility of intestinal carriage of gram-negative bacteria among preterm infants admitted to the neonatal intensive care unit (nicu) in a tertiary teaching hospital in malaysia were determined. a total of 34 stool specimens were obtained from preterm infants upon admission and once weekly up to two weeks during hospitalization. the presumptive colonies of escherichia coli and klebsiella pneumoniae were selected for identification, antibiotic susceptibility test ...201627650884
two types of interleukin 17a-producing γδ t cells in protection against pulmonary infection with klebsiella pneumoniae.klebsiella pneumoniae frequently causes life-threatening infection in children. interleukin 17a (il-17a) is known to be involved in protection against k. pneumoniae infection through activation of neutrophils.201627651419
outbreaks of colistin-resistant and colistin-susceptible kpc-producing klebsiella pneumoniae in a brazilian intensive care unit.carbapenem-resistant enterobacteriaceae (cre), especially those that produce klebsiella pneumoniae carbapenemase (kpc) and are associated with colistin resistance, pose a severe health threat due to the limited treatment options.201627654515
metallo-beta-lactamase producing escherichia coli and klebsiella pneumoniae: a rising threat for hospitalized children.to determine the frequency of metallo-beta-lactamase (mbl) producing e.coli and klebsiella pneumoniae, better phenotypic techniques for mbl detection and choices of treatment available for such cases.201627654721
[effects of antibiotic stewardship on neonatal bloodstream infections].to investigate the effects of antibiotic stewardship on the pathogen and clinical outcome of neonatal bloodstream infections (bsis).201627655532
a review of shv extended-spectrum β-lactamases: neglected yet ubiquitous.β-lactamases are the primary cause of resistance to β-lactams among members of the family enterobacteriaceae. shv enzymes have emerged in enterobacteriaceae causing infections in health care in the last decades of the twentieth century, and they are now observed in isolates in different epidemiological settings both in human, animal and the environment. likely originated from a chromosomal penicillinase of klebsiella pneumoniae, shv β-lactamases currently encompass a large number of allelic vari ...201627656166
fragment-based whole cell screen delivers hits against m. tuberculosis and non-tuberculous mycobacteria.reactive multi-target 'fragment drugs' represent critical components of current tuberculosis regimens. these compounds, such as pyrazinamide, are old synthetic antimycobacterials that are activated inside mycobacterium tuberculosis bacilli and are smaller than the usual drug-like, single-target molecules. based on the success of small 'dirty' drugs in the chemotherapy of tuberculosis, we suggested previously that fragment-based whole cell screens should be introduced in our current antimycobacte ...201627656168
klebsiella pneumoniae necrotizing fasciitis. 201627658424
carbapenem-resistant enterobacteriaceae: prevalence and risk factors in a single community-based hospital in korea.carbapenemase-producing enterobacteriaceae (cpe) are gram-negative bacteria with increasing prevalence of infection worldwide. in korea, 25 cases of cpe isolates were reported by the korea centers for disease control and prevention in 2011. most cpe cases were detected mainly at tertiary referral hospitals. we analyzed the prevalence and risk factors for carbapenem-resistant enterobacteriaceae (cre) in a mid-sized community-based hospital in korea.201627659436
st147 ndm-1-producing klebsiella pneumoniae spread in two tunisian hospitals. 201727659734
green synthesis of silver nanoparticles using pimpinella anisum seeds: antimicrobial activity and cytotoxicity on human neonatal skin stromal cells and colon cancer cells.the present study focused on a simple and eco-friendly method for the synthesis of silver nanoparticles (agnps) with multipurpose anticancer and antimicrobial activities.201727660438
draft genome sequence of klebsiella pneumoniae strain as isolated from zhejiang provincial hospital of tcm, china.klebsiella pneumoniae is a gram-negative, nonmotile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium. here we present draft genome assemblies of klebsiella pneumoniae as, which was isolated in china. the genomic information will provide a better understanding of the physiology, adaptation, and evolution of k. pneumoniae.201627660770
draft genome sequence of a klebsiella pneumoniae strain (new sequence type 2357) carrying tn3926.we present the draft genome sequence of a klebsiella pneumoniae carbapenemase-producing sequence type 2357 (st2357) strain, nb60, which contains drug-resistant genes encoding resistance to beta-lactams, fluoroquinolones, aminoglycosides, trimethoprim-sulfamethoxazole, colistin, macrolides, and tetracycline. strain nb60 was isolated from human blood, making it an important tool for studying k. pneumoniae pathogenesis.201627660779
effect of hand antisepsis agent selection and population characteristics on surgical site infection pathogens.selection of a pre-operative hand antisepsis agent has not been studied in relation to surgical site infection (ssi) culture data. in our hospital, we introduced an alcohol-based hand rub (abr) in 2012 as an alternative to traditional aqueous surgical scrubs (tss). it was the goal of this study to review any effect of this implementation on ssi pathogen characteristics. in addition, we sought to compare our ssi culture data with available national healthcare safety network (nhsn) data. we hypoth ...201627661850
the emerging problems of klebsiella pneumoniae infections: carbapenem resistance and biofilm formation.klebsiella pneumoniae is an opportunistic pathogen that commonly causes nosocomial infections in the urinary tract, respiratory tract, lung, wound sites and blood in individuals with debilitating diseases. klebsiella pneumoniae is still a cause of severe pneumonia in alcoholics in africa and asia, and the predominant primary pathogen of primary liver abscess in taiwan and southeast asia, particularly in asian and hispanic patients, and individuals with diabetes mellitus. in the united states and ...201627664057
draft genome sequence of a ctx-m-15-producing klebsiella pneumoniae sequence type 340 (clonal complex 258) isolate from a food-producing animal.klebsiella pneumoniae carrying blactx-m-15 have been widely disseminated in hospital settings. in this regard, most clinically important strains belong to clonal complex 28 (cc258), which includes sequence type 340 (st340). in this study, we present the draft genome sequence of a ctx-m-15-producing st340 k. pneumoniae strain isolated from a food-producing animal in brazil.201627664870
ndm-producing enterobacteriaceae in a chinese hospital, 2014-2015: identification of ndm-producing citrobacterwerkmanii and acquisition of blandm-1-carrying plasmid in vivo in a clinical escherichia coli isolate.new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae (npe) shows prevalence in china. little is known about the mechanisms related to the spread of npe. recently, a total of 51 non-duplicated npe isolates were collected from a tertiary-care hospital in china and analysed for genetic relatedness by pfge, antimicrobial susceptibility by etest and sequence type by multilocus sequence typing. s1-pfge and southern blot analysis or pcr amplification were used for plasmid profiling. between ...201627667823
bacterial etiology and antibiotic resistance profile of community-acquired urinary tract infections in a cameroonian city.introduction. community-acquired urinary tract infections (cautis) are usually treated empirically. geographical variations in etiologic agents and their antibiotic sensitivity patterns are common. knowledge of antibiotic resistance trends is important for improving evidence-based recommendations for empirical treatment of utis. our aim was to determine the major bacterial etiologies of cautis and their antibiotic resistance patterns in a cosmopolitan area of cameroon for comparison with prescri ...201627667998
control of infectious mortality due to carbapenemase-producing klebsiella pneumoniae in hematopoietic stem cell transplantation.carbapenemase-producing klebsiella pneumoniae (kpc-kp) infections are an emerging cause of death after hematopoietic stem cell transplantation (hsct). in allogeneic transplants, mortality rate may rise up to 60%. we retrospectively evaluated 540 patients receiving a transplant from an auto- or an allogeneic source between january 2011 and october 2015. after an institutional increase in the prevalence of kpc-kp bloodstream infections (bsi) in june 2012, from july 2012, 366 consecutive patients r ...201727668762
acquired resistance to chlorhexidine - is it time to establish an 'antiseptic stewardship' initiative?chlorhexidine digluconate (chg) is an antimicrobial agent used for different types of applications in hand hygiene, skin antisepsis, oral care, and patient washing. increasing use raises concern regarding development of acquired bacterial resistance. published data from clinical isolates with chg minimum inhibitory concentrations (mics) were reviewed and compared to epidemiological cut-off values to determine resistance. chg resistance is rarely found in escherichia coli, salmonella spp., staphy ...201627671220
bladder rupture secondary to emphysematous bladder: a diabetic patient report.we report an emphysematous cystitis in a diabetic patient complicated by peritonitis.201627676715
architecture of class 1, 2, and 3 integrons from gram negative bacteria recovered among fruits and vegetables.the spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. to understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, tomatoes, and carrots). although low levels of resistance have been detected, the bacter ...201627679611
a 2s albumin from the seed cake of ricinus communis inhibits trypsin and has strong antibacterial activity against human pathogenic bacteria.hospital-acquired infections caused by antibiotic-resistant bacteria threaten the lives of many citizens all over the world. discovery of new agents to hinder bacterial development would have a significant impact on the treatment of infections. here, the purification and characterization of rc-2s-alb, a protein that belongs to the 2s albumin family, from ricinus communis seed cake, are reported. rc-2s-alb was purified after protein extraction with tris-hcl buffer, ph 7.5, fractionation by ammoni ...201627680092
scotti: efficient reconstruction of transmission within outbreaks with the structured coalescent.exploiting pathogen genomes to reconstruct transmission represents a powerful tool in the fight against infectious disease. however, their interpretation rests on a number of simplifying assumptions that regularly ignore important complexities of real data, in particular within-host evolution and non-sampled patients. here we propose a new approach to transmission inference called scotti (structured coalescent transmission tree inference). this method is based on a statistical framework that mod ...201627681228
biofilms from klebsiella pneumoniae: matrix polysaccharide structure and interactions with antimicrobial peptides.biofilm matrices of two klebsiella pneumoniae clinical isolates, kpts101 and kpts113, were investigated for their polysaccharide composition and protective effects against antimicrobial peptides. both strains were good biofilm producers, with kpts113 forming flocs with very low adhesive properties to supports. matrix exopolysaccharides were isolated and their monosaccharide composition and glycosidic linkage types were defined. kpts101 polysaccharide is neutral and composed only of galactose, in ...201627681920
activity of cefquinome against extended-spectrum β-lactamase-producing klebsiella pneumoniae in neutropenic mouse thigh model.increasing prevalence of extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae (k. pneumoniae) is of clinical concern. the objective of our study was to examine the in vivo activity of cefquinome against esbl-producing k. pneumoniae strain using a neutropenic mouse thigh infection model. cefquinome kinetics and protein binding in infected neutropenic mice were measured by liquid chromatography-tandem mass spectrometry (lc-ms/ms). dose-fractionation studies over a 24-h dose range o ...201627682189
cd36 provides host protection against klebsiella pneumoniae intrapulmonary infection by enhancing lipopolysaccharide responsiveness and macrophage phagocytosis.klebsiella pneumoniae remains an important cause of intrapulmonary infection and invasive disease worldwide. k. pneumoniae can evade serum killing and phagocytosis primarily through the expression of a polysaccharide capsule, but its pathogenicity is also influenced by host factors. we examined whether cd36, a scavenger receptor that recognizes pathogen and modified self ligands, is a host determinant of k. pneumoniae pathogenicity. despite differences in serum sensitivity and virulence of 3 dis ...201627683817
plasmid-mediated colistin resistance in a patient infected with klebsiella pneumoniae. 201627684336
national survey of colistin resistance among carbapenemase-producing enterobacteriaceae and outbreak caused by colistin-resistant oxa-48-producing klebsiella pneumoniae, france, 2014.from january 2014 to december 2014, 972 consecutive non-replicate carbapenemase-producing enterobacteriaceae isolates from colonised or infected patients were collected at the associated french national reference centre as part of the french national survey on antimicrobial resistance. it included 577 klebsiella spp. (59%), 236 escherichia coli (24%), 108 enterobacter spp. (11%), 50 citrobacter spp. (5%), and a single salmonella spp. isolate (0.1%). of 561 k. pneumoniae isolates, 35 were found t ...201627685838
design, synthesis, and evaluation of pyrazolo-pyrazole derivatives on methylisocitratelyase of pseudomonas aeruginosa: in silico and in vitro study.pseudomonas aeruginosa is an opportunistic micro-organism causing diseases both in animals and humans. in case of human pathology, the role of p. aeruginosa is one of the major concerns in intensive care septicemia. presently, the drug resistance strains of p. aeruginosa are arising mainly by developing multiple mechanisms due to its natural and acquired resistance to many of the antimicrobial agents commonly used in clinical practice. as a result, there is a direct need to invent new drugs so t ...201727686121
in-vivo emergence of polymyxin- b-resistant klebsiella pneumoniae in patients with bloodstream infections. 201627686265
carbapenemase-producing enterobacteriaceae in the uk: a national study (euscape-uk) on prevalence, incidence, laboratory detection methods and infection control measures.to estimate uk prevalence and incidence of clinically significant carbapenemase-producing enterobacteriaceae (cpe), and to determine epidemiological characteristics, laboratory methods and infection prevention and control (ipc) measures in acute care facilities.201727687074
bloodstream infections in febrile neutropenic patients at a tertiary cancer institute in south india: a timeline of clinical and microbial trends through the years.febrile neutropenia (fn) is an oncological emergency. the choice of empiric therapy depends on the locally prevalent pathogens and their sensitivities, the sites of infection, and cost. the infectious diseases society of america guidelines are being followed for the management of fn in india.201727688611
preparation of thermosensitive gel for controlled release of levofloxacin and their application in the treatment of multidrug-resistant bacteria.levofloxacin is a synthetic broad-spectrum antibacterial agent for oral or intravenous administration. chemically, levofloxacin is the levorotatory isomer (l-isomer) of racemate ofloxacin, a fluoroquinolone antibacterial agent. quinolone derivatives rapidly and specifically inhibit the synthesis of bacterial dna. levofloxacin has in vitro activity against a broad range of aerobic and anaerobic gram-positive and gram-negative bacteria. however, formulation of combined poloxamers thermoregulated ( ...201727689094
increase in prevalence of kpc-2-producing klebsiella pneumoniae recovered from respiratory secretions of intensive care patients-getting a free ride on a menacing colistin resistance. 201627691984
synthesis and antimicrobial activity of small cationic amphipathic aminobenzamide marine natural product mimics and evaluation of relevance against clinical isolates including esbl-carba producing multi-resistant bacteria.a library of small aminobenzamide derivatives was synthesised to explore a cationic amphipathic motif found in marine natural antimicrobials. the most potent compound e23 displayed minimal inhibitory concentrations (mics) of 0.5-2μg/ml against several gram-positive bacterial strains, including methicillin resistant staphylococcus epidermidis (mrse).e23 was also potent against 275 clinical isolates including staphylococcus aureus, enterococcus spp., escherichia coli, pseudomonas aeruginosa, and k ...201627692769
evaluation of antimicrobial, cytotoxic, and hemolytic activities from venom of the spider lasiodora sp.this study characterized the protein/peptide profile of venom isolated from the spider lasiodora sp. (mygalomorphae, theraphosidae) found in northeastern brazil and determined its antimicrobial activity, toxicity against human cells, and hemolytic activity. protein concentration of the lasiodora sp. venom was 4.53 ± 0.38 mg/ml. sds-page showed proteins with molecular masses up to 75 kda, some of which contained disulfide bridges. rp-hplc analysis separate at least 12 peaks that were identified b ...201627693304
toxicity mechanism of titanium dioxide and zinc oxide nanoparticles against food pathogens.food preservation is an important field of research. it extends the shelf life of major food products. our current study is based on food preservation through tio2 and zno nanoparticles. tio2 and zno are biocompatible nanomaterial. the biocompatibility of the materials were established through toxicity studies on cell lines. titanium dioxide and zinc oxide nanoparticle were synthesized by wet chemical process. they are characterized by x-ray diffraction and tem. the antibacterial activities of b ...201627694049
microangiopathic antiphospholipid antibody-associated syndrome in a pregnant lady.we describe a gravid 37-year-old chinese lady with known triple positive primary antiphospholipid syndrome with previous recurrent deep vein thrombosis and early spontaneous miscarriages. she was managed with low-molecular weight heparin, aspirin, hydroxychloroquine, prednisolone and monthly intravenous immunoglobulin. she presented with recurrent per-vaginal bleeding at 22 weeks of gestation and was found to have abruptio placentae. anti-coagulation was held off. she subsequently delivered a st ...201727694537
the influence of the autochthonous wastewater microbiota and gene host on the fate of invasive antibiotic resistance genes.the aim of this study was to assess the fate of invasive antibiotic resistance genes (arg) discharged in wastewater. with this objective, antibiotic resistant bacteria (arb) known to harbor specific arg were inoculated in wastewater (hospital effluent, or municipal raw and treated wastewater) and in ultra-pure sterile water microcosms. two sets of wastewater arb isolates were used - set 1, enterococcus faecalis, acinetobacter johnsonii, klebsiella pneumoniae and set 2, enterococcus faecium, acin ...201727697350
risk factors, outcomes, and mechanisms of tigecycline-nonsusceptible klebsiella pneumoniae bacteremia.a rise in tigecycline resistance in klebsiella pneumoniae has been reported recently worldwide. we sought to identify risk factors, outcomes, and mechanisms for adult patients with tigecycline-nonsusceptible k. pneumoniae bacteremia in taiwan. we conducted a matched case-control study (ratio of 1:1) in a medical center in taiwan from january 2011 through june 2015. the cases were patients with tigecycline-nonsusceptible k. pneumoniae bacteremia, and the controls were patients with tigecycline-su ...201627697759
a model for predicting nosocomial carbapenem-resistant klebsiella pneumoniae infection.mortality associated with infections due to carbapenem-resistant klebsiella pneumoniae (cr-kp) is high and the infections need to be predicted early. the risk factors for cr-kp infection are heterogeneous. the aim of the present study was to construct a model allowing for the early prediction of cr-kp infection. nosocomial infections due to k. pneumoniae were evaluated retrospectively over a 2-year period. the case cohort consisted of 370 inpatients with cr-kp infection. for each case enrolled, ...201627699021
allogeneic stem cell transplantation in a blast-phase chronic myeloid leukemia patient with carbapenem-resistant klebsiella pneumoniae tricuspid valve endocarditis: a case report.in chronic myeloid leukemia (cml), the occurrence of blastic transformation is rare. treatment outcome is generally poor. allogeneic stem cell transplantation (allo-sct) is the only potentially curative treatment option for advanced-phase cml. infections caused by carbapenem-resistant klebsiella pneumoniae (crkp) isolates are associated with high morbidity and mortality rates, particularly in patients with haematological malignancies. infection and colonization by these multiresistant bacteria m ...201627699025
distribution of esbls, ampc β-lactamases and carbapenemases among enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the asia-pacific region during 2008-14: results from the study for monitoring antimicrobial resistance trends (smart).to investigate the antimicrobial resistance and assess the molecular characteristics of β-lactamases (esbls, ampc β-lactamases and carbapenemases) among enterobacteriaceae isolates that caused intra-abdominal infections (iais) in patients hospitalized in the asia-pacific region during 2008-14.201727703058
community-onset bloodstream and other infections, caused by carbapenemase-producing enterobacteriaceae: epidemiological, microbiological, and clinical features.background.  because most infections caused by carbapenemase-producing enterobacteriaceae (cpe) begin during hospitalization, there are limited data about community-onset (co) infections caused by cpe. our aim is to describe the frequency of co infections caused by cpe as well as the clinical features of co bloodstream infections (co-bsis). methods.  this study includes retrospective case series of co infections caused by cpe in a tertiary hospital from january 2010 to july 2014. any clinical sa ...201627703997
extended-spectrum ß-lactamase-producing enterobacteriaceae as a common cause of urinary tract infections in sri lanka.extended-spectrum ß-lactamase-producing enterobacteriaceae (esbl-pe) are increasingly reported as pathogens in urinary tract infections (utis). however, in sri lanka, the clinical and molecular epidemiology of esbl-pe implicated in utis has not been well described.201627704730
development of a colloidal gold-based immunochromatographic strip for rapid detection of klebsiella pneumoniae serotypes k1 and k2.in this study, a novel colloidal gold-based immunochromatographic strip (ics) containing anti-klebsiella pneumoniae capsular polysaccharide polyclonal antibodies was developed to specifically detect k. pneumoniae serotypes k1 and k2. capsular polysaccharide k1 and k2 antigens were first used to produce polyclonal anti-k1 and anti-k2 antibodies. reference strains with different serotypes, nontypeable k. pneumoniae strains, and other bacterial species were then used to assess the sensitivity and s ...201627707941
efficacy of ceftolozane/tazobactam against urinary tract and intra-abdominal infections caused by esbl-producing escherichia coli and klebsiella pneumoniae: a pooled analysis of phase 3 clinical trials.the increase in infections caused by drug-resistant esbl-producing enterobacteriaceae (esbl-ent) is a global concern. the characteristics and outcomes of patients infected with esbl-ent were examined in a pooled analysis of phase 3 clinical trials of ceftolozane/tazobactam in patients with complicated urinary tract infections (aspect-cuti) and complicated intra-abdominal infections (aspect-ciai).201727707990
development of an in vitro assay, based on the biofilm ring test(®), for rapid profiling of biofilm-growing bacteria.microbial biofilm represents a major virulence factor associated with chronic and recurrent infections. pathogenic bacteria embedded in biofilms are highly resistant to environmental and chemical agents, including antibiotics and therefore difficult to eradicate. thus, reliable tests to assess biofilm formation by bacterial strains as well as the impact of chemicals or antibiotics on biofilm formation represent desirable tools for a most effective therapeutic management and microbiological risk ...201627708625
risk factor analysis of ciprofloxacin-resistant and extended spectrum beta-lactamases pathogen-induced acute bacterial prostatitis in korea.the objectives of this study were to investigate risk factors and the incidence of ciprofloxacin resistance and extended-spectrum beta-lactamases (esbl) in patients with acute bacterial prostatitis (abp). we reviewed the medical records of 307 patients who were diagnosed with abp between january 2006 and december 2015. the etiologic pathogens and risk factors for ciprofloxacin-resistant e. coli and esbl-producing microbes, susceptibility to ciprofloxacin, and the incidence of esbl in patients wi ...201627709861
a biodegradable polycationic paint that kills bacteria in vitro and in vivo.bacterial colonization and subsequent formation of biofilms onto surfaces of medical devices and implants is a major source of nosocomial infections. most antibacterial coatings to combat infections are either metal-based or nondegradable-polymer-based and hence limited by their nondegradability and unpredictable toxicity. moreover, to combat infections effectively, the coatings are required to display simultaneous antibacterial and antibiofilm activity. herein we report biocompatible and biodeg ...201627709890
in vitro antibacterial activity of rhodanine derivatives against pathogenic clinical isolates.bacterial infections present a serious challenge to healthcare practitioners due to the emergence of resistance to numerous conventional antibacterial drugs. therefore, new bacterial targets and new antimicrobials are unmet medical needs. rhodanine derivatives have been shown to possess potent antimicrobial activity via a novel mechanism. however, their potential use as antibacterials has not been fully examined. in this study, we determined the spectrum of activity of seven rhodanine derivative ...201627711156
[first case of ceftazidime/avibactam administration in home care. esbl producing klebsiella pneumoniae bacteremia]. 201627712848
early detection of oxa-370-producing klebsiella pneumoniae st17 co-harboring blactx-m-8 in brazil.we aimed to describe an early detection of oxa-370-producing klebsiella pneumoniae in brazil. the isolate ccbh10079 belonged to st17 and the blaoxa-370 was located in a plasmid of ≅57kb.201627712929
the contribution of capsule polysaccharide genes to virulence of klebsiella pneumoniae. 201627715471
carbapenem-resistant klebsiella pneumoniae influences the outcome of early infections in liver transplant recipients.infections remain a leading cause of morbidity and mortality among liver transplant (lt) recipients. the aim of our study was to define the factors associated with outcome of early bacterial and fungal infections in a cohort of patients who underwent lt at the university hospital of ancona over a nine year period.201627716164
pharmacokinetics of ceftazidime-avibactam in two patients with kpc-producing klebsiella pneumoniae bacteremia and renal impairment.limited data exist regarding optimal dosing of ceftazidime/avibactam (c/a) in patients with unique physiology, who were excluded from published clinical trials. data are also lacking regarding clinical efficacy of c/a in patients with infections due to multidrug-resistant gram-negative pathogens. to expand knowledge in these areas, we present pharmacokinetic data from two patients with klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae bloodstream infections, both of whom had rena ...201627716983
healthcare-associated gram-negative bloodstream infections: antibiotic resistance and predictors of mortality.this article describes the prevalence of antibiotic resistance and predictors of mortality for healthcare-associated (ha) gram-negative bloodstream infections (gn-bsi). in total, 831 cases of ha gn-bsi from 17 intensive care units in different centres in turkey were included; the all-cause mortality rate was 44%. carbapenem resistance in klebsiella pneumoniae was 38%, and the colistin resistance rate was 6%. multi-variate analysis showed that age >70 years [odds ratio (or) 2, 95% confidence inte ...201627717604
biliary diseases as main causes of pyogenic liver abscess caused by extended-spectrum beta-lactamase-producing enterobacteriaceae.little is known about aetiology and morbidity and clinical characteristics of pyogenic liver abscess caused by extended-spectrum beta-lactamase-producing enterobacteriaceae.201627718321
[the clinical feature and outcomes of severe aplastic anemia patients suffered from bacteremia following antithymocyte globulin].objective: to assess the clinical feature and outcomes of severe aplastic anemia (saa) patients suffered from bacteremia following antithymocyte globulin (atg). methods: a total of 264 cases hospitalized in our hospital between jan 2000 and july 2011 were enrolled into this study. we evaluated the associated pathogens of bacteremia, analyzed the risk factors by logistic regression and estimated the overall survival (os) by kaplan-meier method for the cohort of patients. results: bloodstream infe ...201627719726
morphological changes induced by β-lactam antibiotics observed on gram staining. 201727720866
molecular detection of carbapenemase-producing genes in referral enterobacteriaceae in south africa: a short report.molecular confirmation of carbapenemase-producing enterobacteriaceae (cpe) was introduced in south africa (sa) at the end of 2011. we report on the detection of these resistance genes based on referral isolates. enterobacteriaceae with non-susceptibility to any of the carbapenems according to defined criteria for antimicrobial susceptibility testing results were sent to a reference laboratory. a proportion of isolates had limited demographic, epidemiological and clinical data available. organism ...201627725012
distribution of virulence genes and genotyping of ctx-m-15-producing klebsiella pneumoniae isolated from patients with community-acquired urinary tract infection (ca-uti).klebsiella pneumoniae is one of the most important agents of community-acquired urinary tract infection (ca-uti). in addition to extended-spectrum β-lactamases (esbls), a number of virulence factors have been shown to play an important role in the pathogenesis of k. pneumoniae, including capsule, siderophores, and adhesins. little is known about the genetic diversity and virulence content of the ctx-m-15-producing k. pneumoniae isolated from ca-uti in iran. a total of 152 k. pneumoniae isolates ...201627725280
chitosanase purified from bacterial isolate bacillus licheniformis of ruined vegetables displays broad spectrum biofilm inhibition.a number of bacterial species produces chitosanases which has variety of applications because of its high biodegradability, non-toxicity and antimicrobial assets. in the present study chitosanase is purified from new bacterial species bacillus licheniformis from spoiled vegetable. this novel strain of bacillus licheniformis isolated from spoilt cucumber and pepper samples has the ability to produce the chitosanase enzyme when grown on chitosan substrate. study also examined its antibiofilm prope ...201627725283
community acquired klebsiella pneumonia related psoas abscess. 201627728379
nosocomial outbreak of kpc-2- and ndm-1-producing klebsiella pneumoniae in a neonatal ward: a retrospective study.the spread of resistance to carbapenems among enterobacteriaceae has become a major public health problem in recent years. in this study, we describe an outbreak of klebsiella pneumoniae in the neonatal ward. first, we aimed to study the drug resistance, genetic relatedness, and transmission mechanism of carbapenem-resistant k. pneumoniae; second, we implemented infection control measures to contain the outbreak.201627733128
effects of scutellaria baicalensis on activity and biofilm formation of klebsiella pneumoniae(△).<strong>objective</strong> to explore the effects of scutellaria baicalensis on activity and biofilm formation of klebsiella pneumonia (kp).<strong>methods</strong> the broth and agar dilution methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of scutellaria baicalensis for tw518. vitek-32 system was used to assay tw518 susceptibility to antibiotics. kp biofilms were formed in vitro and stained with baclight live/dead stain. the class in ...201627733226
solid organ transplant antibiograms: an opportunity for antimicrobial stewardship.we aimed to compare the antimicrobial susceptibility percentages in blood and urine bacterial isolates recovered from solid organ transplant (sot) recipients with those reported in the hospital-wide antibiogram.201627733304
patients with culture negative pyogenic liver abscess have the same outcomes compared to those with klebsiella pneumoniae pyogenic liver abscess.etiologic organism is not frequently isolated despite multiple blood and fluid cultures during management of pyogenic liver abscess (pla). such culture negative pyogenic liver abscess (cnpla) is routinely managed by antibiotics targeted to klebsiella pneumoniae. in this study, we evaluated the outcomes of such clinical practice.201627733320
the role of the pyruvate acetyl-coa switch in the production of 1,3-propanediol by klebsiella pneumoniae.pyruvate dehydrogenase-complex (acoabcd) and pyruvate formate-lyase (pfl) are two pathways responsible for synthesis of acetyl-coa from pyruvate (pyruvate acetyl-coa switch). the two pathways were individually deleted in klebsiella pneumoniae, and the role of the pyruvate acetyl-coa switch in 1,3-propanediol production was investigated. fermentation results showed that the two pathways were both active in the wild-type strain. acetyl-coa formation between the two pathways was equal in the wild-t ...201727734285
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