the role of intra-typic variations of antigenic pattern in the typing of erysipelothrix rhusiopathiae strains. 197789805
steroid secretion by in vitro perfused testes: inhibitors of testosterone biosynthesis. 197989816
effect of encephlitogenic protein. ppd and tetanus toxoid on leukocyte migration in agarose. a study of "cross-reactivity".the reactivity to three antigens: bovine encephalitogenic protein (ep), ppd, and tetanus toxoid, was studied with blood leukocytes from healthy humans using clausen's (5) leukocyte migration in agarose technique. there is an obvious correlation between the reaction of ep (all concentrations studied) and to low concentrations of ppd; and between the reactivity to low concentrations of ep and low concentrations of tetanus toxoid. after vaccination with tetanus vaccine, a marked increase in reactiv ...197989821
antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion. 197989825
inhibition of histamine release from rat mast cells by cytochalasin a and other sulfhydryl reagents. 197989848
[alterations in pharmacokinetics during toxicity tests (author's transl)].some possible reasons are discussed which may lead to changes in the pharmacokinetics of a drug during long-term toxicity tests. since most of these alterations have toxicological consequences, their evaluation can be helpful in the interpretation of toxicity studies. furthermore, and altered pharmacokinetic profile may be a diagnostic tool for the detection of organ damage (e.g., eliminatory organs). in this paper three examples of the "radioactive test dose method" are shown. this method allow ...197889849
a radioimmunoassay for erythropoietin.we describe the development of a radioimmunoassay (ria) for erythropoietin. antisera were raised in rabbits with an impure human urinary erythropoietin preparation used as immunogen, but with pure human erytropoietin serving as the labeled antigen in the ria and as a primary standard. the immunoreactivity of erythropoietin is not altered significantly by the mode of labeling with radioiodine, even though the biologic activity is lost. with this method, it is possible to detect 2--3 mu of erythro ...197989877
use of pm-2 dna degradation as a pharmacokinetic assay for bleomycin.the pm-2 dna fluorescence assay has been shown to be a rapid, sensitive, and reproducible assay for bleomycin biochemical activity. the assay can detect bleomycin in human serum in the nmol range. the method measures dna degradative activity of bleomycin and could be used to determine activity of bleomycin analogs and metabolites. the usefulness of the assay to perform bleomycin pharmacokinetic studies in cancer patients has been demonstrated. linear regression analyses of parallel bleomycin ass ...197989893
ultrastructural and autoradiographic study of the effects of bleomycin on the interphase nucleus of cultured normal cells.primary cultures of hepatocytes and epithelial endometrial cells were treated with bleomycin (10 to 200 microgram/ml) for 30 to 300 min. structural changes were studied with a staining method which contrasts ribonucleoproteins. the earliest visible alteration was the accumulation of perichromatin granules in association with the nucleolus. this disturbance was frequently accompanied by modifications in the nucleolar architecture. after larger treatments, the most striking changes were nucleolar ...197989895
modulation of cellular interactions between c3h/10t1/2 cells and their transformed counterparts by phosphodiesterase has been demonstrated previously that nontransformed c3h/10t1/2cl8 mouse embryo fibroblasts (10t1/2) can induce a state of reversible growth inhibition in cocultured malignantly transformed mouse fibroblasts and that this inhibition is modulated by serum concentration. the present study suggests that cyclic nucleotides may be implicated in this intercellular communication. the phosphodiesterase inhibitors theophylline, caffeine, and 3-isobutyl-1-methylxanthine (ibx) at concentrations of 10(-3 ...197989899
alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability.the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ...197989900
rat alpha-macrofetoprotein (acute-phase alpha 2-macroglobulin) during hepatocarcinogenesis.rat alpha-macrofetoprotein (amf) and alpha-fetoprotein (afp) are secreted by the fetal liver and become elevated in serum during hepatocarcinogenesis and in animals bearing hepatocellular carcinomas. it has been suggested that these fetal plasma proteins may be influenced by related control mechanisms. the experiments presented herein examined the early responses of these plasma proteins during hepatocarcinogenesis using the hepatocarcinogens acetylaminofluorene and diethylnitrosamine. under the ...197989903
coupling between neuronal activity and focal blood flow in experimental seizures.local blood flow, ecog and single cortical neurone activity were recorded simultaneously from single microelectrodes in 17 cats. seizures were induced by repeated intravenous injections of pentylenetetrazol (ptz, 10-20 mg/kg) or by local application of 1 m na-penicillin. seven to 20 sec after appearance of burst activity in cortical neurones and ecog, focal flow increased up to 300% of control. the extent of this flow increase was significantly correlated with the change in firing rate of the ne ...197989943
retrograde axonal transport of horseradish peroxidase in regenerating sciatic nerve of the rat. 197989965
surface characteristics of chicken peripheral b and t lymphocytes defined by specific heteroantisera. 197989975
neuroimmunologic disease: experimental and clinical aspects. 197990005
the cellular basis of transplantation tolerance in the rat. 197990010
isolation and characterization of diploid clones from adult and newborn rat liver cell lines.a high frequency of diploid and near-diploid clones were developed from cell lines derived from adult and newborn rat liver using micropipettes. there were some differences in morphology, biochemical properties and growth rate between clones. cloned cells had low levels of tyrosine transaminase activity, glucose-6-phosphatase activity and albumin content. a diploid clone and pseudodiploid clone derived from adult rat liver cell line were positive for alpha-fetoprotein.197990011
revertants of adenovirus type-12-transformed hamster cells have lost part of the viral genomes. 197990022
distribution of myosin isoenzymes among skeletal muscle fiber types.using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ...197990047
anionic sites in the glomerular basement membrane. in vivo and in vitro localization to the laminae rarae by cationic probes.cationized ferritin (cf) of narrow pi range (7.3-7.5) and the basic dye ruthenium red (rr) have been used as cationic probes to partially characterize anionic sites previously demonstrated in the glomerular basement membrane (gbm). when cf was given i.v. to normal rats and the left kidney was fixed by perfusion 15 min thereafter, clusters of cf molecules were found throughout the lamina rara interna (lri), lamina rara externa (lre), and mesangial matrix distributed at regular (approximately 60 n ...197990048
immunological studies of the embryonic muscle cell surface. antiserum to the prefusion myoblast.xenogeneic antisera raised in rabbits have been used to detect compositional changes at the cell surfaces of differentiating embryonic chick skeletal muscle. in this report, we present the serological characterization of antiserum (anti-m-24) against muscle tissue and developmental stage-specific cell surface antigens of the prefusion myoblast. cells from primary cultures of 12-d-old embryonic chick hindlimb muscle were injected into rabbits, and the resulting antisera were selectively absorbed ...197990049
enzymatic tracers in the study of vascular permeability.elucidation of the ultrastructural basis of vascular permeability was aided by the development of cytochemical techniques for visualizing the distribution, within the vessel wall, of intravenously injected peroxidatic enzymes of varying molecular size. tracer enzymes available range from 10 a (hemeoctapeptide) to 52 a (catalase) effective molecular radius. the use of enzymatic probe molecules assumes a thorough characterization of: (a) the molecular charge (isoelectric point of the native enzyme ...197990073
the use of avidin-biotin interaction in immunoenzymatic techniques.biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. based on avidin-biotin interaction, the labeled proteins were used in quantitative enzyme-immunoassay and enzyme-immunohistochemical staining procedures. two procedures were developed. in the first procedure, named the bridged avidin-biotin (brab) technique four steps were used sequentially in order to quantify or ...197990074
generation of h-2 restricted cytotoxic t cells by trinitrophenylated proteins in vitro: specificity and requirements. 197990099
m467: a murine iga myeloma protein that binds a bacterial protein. i. recognition of common antigenic determinants on salmonella flagellins.we have studied the binding of m467, an iga murine myeloma protein, to flagellin from seven species of salmonella. it was found that m467 was reacting with antigenic determinants that were common to all the flagellins studied. these determinants were not related to serotypic antigens. electronmicrographs of unreduced m467 showed a variety of polymeric species bound to flagella in a manner that could produce immobilization as well as agglutination and precipitation through cross-linking of antige ...197990100
specific absorption of t lymphocytes committed to soluble protein antigens by incubation on antigen-pulsed macrophage monolayers.monolayers of macrophages (mphi) pulsed with antigen were used as immunosorbents for t lymphocytes from guinea pigs primed to soluble protein antigens. t lymphocytes were cultured on the mphi monolayers for 4 hr, then aspirated and reincubated on a fresh monolayer pulsed with the same antigen for a second and a third step. t lymphocytes so treated were selectively deprived of cells responding in assay for antigen-dependent proliferation against the antigen used for pulsing the absorbing monolaye ...197990101
characterization of in vitro proliferative responses of human lymphocytes to leishmanial antigens.leishmaniasis is an intracellular protozoal infection for which host defense is believed to depend on cellular immune mechanisms. the in vitro proliferative responses of lymphocytes from patients with leishmaniasis and from control subjects to leishmanial antigens were examined. only lymphocytes from patients responded to 1 microgram of leishmanial antigen/ml, whereas both patient and control lymphocytes responded to 10 micrograms/ml. the nonspecific responses were most likely not due to a mitog ...197990105
selective vascular thrombosis induced by a direct electrical current: animal experiments. first clinical trials. 197890140
differential growth inhibition in two human carcinoma cell lines by cyclic adenosine 5'-monophosphate analogs.the inhibition of cell replication in two human carcinoma cell lines by various cyclic amp analogs was explored. in all instances, the addition of the cyclic nucleotide phosphodiesterase inhibitor 1-methyl-3-isoburylxanthine resulted in synergistic growth inhibition by the analogs. a correlation was found between an analog's ability to inhibit growth and its ability to activate protein kinase. a differential effect of the breakdown product 8-bromo-amp (8-bramp) on cell replication in the two cel ...197990151
identification of the primate papovavirus hd as the stump-tailed macaque virus.the recently isolated primate papovavirus hd is shown to be indistinguishable from the stump-tailed macaque virus by immunofluorescent reactivity, by restriction endonuclease analysis, and by nucleic acid hybridization assay.197990163
[micromethod of carrying out the passive hemagglutination reaction for detecting antibodies to influenza a2 virus in tears and aqueous humor]. 197990190
gamma-aminobutyric acid in human cerebrospinal fluid: normal values. 197990262
rubella screening by hemolysis-in-gel-test. 197990314
[species and interspecies antigenic specificity of house dust mites (genus dermatophagoides)]. 197990327
central transport and distribution of labelled glutamic and aspartic acids to the cochlear nucleus in cats: an autoradiographic study. 197990345
detection by antihapten antibodies of liver-bound compounds related to azocarcinogens or their metabolites.the localization of known azocarcinogens and metabolites such as p-aminoazobenzene and n-methyl-p-aminoazobenzene bound to components of liver cells of rats fed single or multiple doses of 3'-methyl-p-dimethylaminoazobenzene has been determined with the use of antibodies raised against p'-azo-p-aminoazobenzene and p'-azo-n-monomethyl-p-aminoazobenzene in the indirect fluorescent antibody procedure. these 2 antisera reacted with liver cells of rats fed 3'-methyl-p-dimethylaminoazobenzene, p'-amin ...197990352
[pharmacological properties and antitumor activity of a preparation diiodo-benzo-tepa in experiments].experimental data on the pharmacokinetics, toxicity, antitumor activity of the antitumor drug diiodo-benzo-tepa are reported.197990429
cross-neutralization tests on sheep pox, goat pox and contagious pustular dermatitis cross-neutralization tests in cell cultures, sheep pox and contagious pustular dermatitis (cpd) hyperimmune sera did not cross-react in the heterologous system. but goat pox hyperimmune serum neutralized both sheep pox and cpd viruses revealing a one-way serological relationship of goat pox virus with both sheep pox and cpd viruses.197990458
isolation and immunological reactivity of soluble fractions from rat seminiferous tubule basement membrane.a preparation rich in basement membranes isolated from rat testes (stbm) was exposed to pepsin, collagenase, trypsin, and pronase to obtain soluble fractions. the immunological reactivity of these fractions was studied by gel immunodiffusion or by passive hemagglutination tests against an anti-stbm serum. all fractions reacted with the antiserum, but the highest titer was detected when the antiserum was reacted with a fraction that contained only traces of hydroxyproline (fraction 1), whereas lo ...197990490
relations between fatty acids and oestrogen binding properties of pure rat alpha 1-foetoprotein.a delipidation procedure based on treatment with charcoal at ph 3 has been applied to highly purified rat alpha 1-foetoprotein preparations. the oestrogen binding properties of the delipidated proteins have been studied with an equilibrium dialysis technique, and compared with the properties of the untreated foetal protein, as well as those of preparations reconstituted from the defatted alpha 1-foetoprotein and the removed lipids. an important increase has been evidenced for the binding levels ...197990528
cells of origin of the spinocerebellar tract in the rat, studied with the method of retrograde transport of horseradish peroxidase.following injections of horseradish peroxidase into the cerebellum, the distribution of labeled neurons was studied in the whole length of the spinal cord of the rat. to find the ascending side of the axons, injections were made following hemisections at c1 or between c1 and c2. labeled spinocerebellar tract neurons were classified into two groups according to the axonal course in the spinal cord; one is composed of neurons with uncrossed ascending axons and the other, neurons with crossed ascen ...197990539
in vitro studies of the replication of moloney murine leukemia virus. 197990575
biological role of gramicidin s in spore functions. studies on gramicidin-s-negative mutants of bacillus brevis atcc9999.gramicidin-s-negative mutants of bacillus brevis atcc9999 have been isolated with a remarkly higher yield after ethidium bromide or acridine orange treatment, than after n-methyl-n'-nitro-n-nitrosoguanidine treatment. four (miv, smr170, r5 and eb 16) of 38 isolated mutants were characterized with respect to the lesion in gramicidin-s-synthesizing activity. the mutants sporulate to the same extent as the parental strain except mutant smr 170 which sporulates less. however, mutant spores were more ...197990616
relative affinity of antisera for myelin basic protein (mbp) and degree of affinity heterogeneity. 197990641
the location and expression of idiotypic determinants in the immunoglobulin variable region--i. characterization of antibodies directed against the variable region of mouse myeloma immunoglobulins 315 and 460. 197990644
[animal experimental studies on the volume expanding effect of hydroxyethyl starch 40000 in the dog acute hemorrhagic shock].after acute hemorrhagic shock in dogs the hemodynamics of hydroxyethyl starch (mw: 40,000) are studied. the experiment shows that this plasma expander has a good efficiency for 3 to 4 hours. the indications of application of this colloidal solution are discussed.197990651
characterization of the antigenic determinants of cholera toxin subunits.the antigenic specificity of purified preparations of a subunit, b subunit, alpha chain, and gamma chain of cholera toxin was studied by double immunodiffusion and radioimmunoassay with antisera produced in rabbits and mice. rabbits immunized with a subunit produced serum antibodies which were capable of binding radiolabeled a subunit, alpha chain, and b subunit. rabbits immunized with alpha chain produced serum antibodies that would bind radiolabeled alpha chain and a subunit. rabbits immunized ...197990654
the indirect rat mast cell degranulation test reconsidered.the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ...197990655
complement profiles in monkeys subjected to aggregate (immune complex) anaphylaxis, and following injection of soluble and particulate polysaccharides.complement profiles were established in four groups of macaca irus monkeys: (i) aggregate (immune complex) anaphylaxis was induced following immunization, with ovalbumin. upon challenge, systemic arterial pressure decreased from 115 to 50 mm hg (mean values) in 10 min. the complement profiles revealed decreases in: c1q to less than 10% of initial value within 5 min; c4 proportional to hypotension; c3 slowly to 60% at 24 h; c5, c6, c7, c8 and factor b to about 80% of initial value in 5--30 min. c ...197990656
antigens in penicillin allergy. iv. induction of ige antibody response in mice after daily treatment with contaminated but not with pure penicillin. 197990657
establishment, characterization and virus expression of cell lines derived from radiation- and virus-induced lymphomas of c57bl/ka mice.permanent cell lines have been established in vitro from lymphoid tumors induced in c57bh/ka mice by fractionated x-irradiation or by inoculation of the radiation leukemia virus (radov). the cultured cells are lymphoblastic, replicate rapidly in vitro, and are tumorigenic in vivo. the cell surface markers thy 1, ly 1, ly 2,3 and gix are expressed by the lymphoid tumor cells in the mouse and persist in the corresponding cell lines; expression of the h-2 and tl antigens is greatly reduced during i ...197990660
tobacco leaf mesophyll peroxidase. isolation, purification and some properties.two isoperoxidases, p1 and p2, were isolated from tobacco mesophyll leaves by the combined methods of ammonium sulphate precipitation, page and deae-cellulose column chromatography. the ph otpima for p1 and p2 were 7.0 and 7.5 respectively. the values of km for h2o2 were 1.82 and 0.87 mm for p1 and p2. results from this investigationsuggest that these isoperoxidases differ in their electrophoretic, catalytic and physical properties.197990669
purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity.a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c. was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ...197990677
on the kinetics and maximal capacity of the system for rapid axonal transport in mammalian neurones.1. rabbit peroneal nerves were incubated in vitro in two-compartment chambers. step-gradients of temperature were established so that the proximal part of each nerve was slightly warmer than the distal part. after incubation, the distribution of dopamine-beta-hydroxylase (dbh) activity along the nerves was examined as an indication of the behaviour of rapid transport in adrenergic axons. 2. with temperature gradients of 5 and 8 degrees c, transport velocity in the proximal regions was expected f ...197990719
lymphocyte viability and function following ex-vivo splenic perfusions in dogs. 197990722
lymphocyte alteration by procainamide: relation to drug-induced lupus erythematosus syndrome.sera from 11 (65%) of 17 patients with newly diagnosed procainamide-induced lupus contained cold-reactive lymphocytotoxic antibodies to normal human lymphocytes in titres of 1/2 to 1/128. in contrast, only 3 of 15 patients on long-term procainamide therapy without lupus and 3 of 65 normal men had serum lymphocytotoxic antibodies, none at a titre higher than 1/2. antibody levels in the lupus patients declined quickly after procainamide was stopped, in parallel with their clinical improvement. pro ...197990917
rubella: which vaccine? 197990959
human beta-thromboglobulin inhibits pgi2 production and binds to a specific site in bovine aortic endothelial cells. 197991112
stationary potential of the brain: part i. basic studies. 197991122
[efficacy of plasma substitutes of different molecular weight in acute haemorrhagic shock in dogs (author's transl)].dogs were bled into haemorrhagic shock. they were then given isovolaemic infusions of dextran 60 and 40 and of hydroxyethyl starch with an average molecular weight of 200,000 and 40,000 respectively with a view of assessing the haemodynamic efficacy of these plasma substitutes. solutions of high molecular weight hydroxyethyl starch (hes 400) were retained in the circulation for about the same length of time as was dextran 60. hes 40 (molecular weight 40,000) was retained for about 3-4 hours as m ...197991161
cell-free synthesis and segregation of beta 2-microglobulin.beta2-microglobulin has been synthesized in vitro by using a rabbit reticulocyte lysate system and mrna from the mouse tumor cell line el4. the molecule is synthesized as a precursor with an nh2-terminal extension of 19 amino acids: ser-x-ser-val-x-leu-val-phe-leu-val-leu-val-ser-leu-x-gly-leu-tyr-x. the processing and segregation of this peripheral membrane protein are directly comparable to those of secretory proteins and integral membrane proteins: addition of dog pancreas microsomal membrane ...197991168
the chemistry of the brain. 197991194
characterization of reverse transcriptase from filine leukemia virus by radioimmunoassay. 197991258
a study of the muscle damage caused by the 7.62 nato rifle. 197991287
lymphocyte transformation in the presence of myelin basic protein in multiple sclerosis and control subjects.the transformation of lymphocytes in vitro in the presence of human myelin basic protein has been investigated in normal people, patients with multiple sclerosis (ms) and controls with other neurological diseases. there was little or no response at low concentrations (1--10 microgram/ml) but significant transformation at higher concentrations (100--1000 microgram/ml) in all three groups. there was no significant difference among the groups as a whole, but those ms patients who had had disease fo ...197991302
the zinc-iodide-osmium technique in studies of the neurosecretory system in rats. 197991310
connectivity of the auditory forebrain nuclei in the guinea fowl (numida meleagris).injection of tritiated leucine and proline into the nucleus ovoidalis of the guinea fowl (numida meleagris) produces terminal labeling in the palaeostriatum and in three adjacent zones (field l1-l3) of the auditory neostriatum (an). l2, situated between l1 and l3, receives the main input and corresponds to the former field l of rose. these neuroanatomically defined zones of the auditory neostriatum are also characterized by differing properties of their neurons. injection of radioactive material ...197991442
fine specificity of a continuously growing killer cell clone specific for h-y antigen.h-y-specific cytotoxic t cells were first cloned in soft agar and grown over a period of 8 months in media conditioned with supernatants from mouse and rat spleen cells stimulated with concanavalin a. the specificity of cloned cells and their cytolytic potential remained essentially unchanged over the entire culture period. in addition to lysing male target cells expressing h-2db antigens, the cytolytic cells lysed also male as well as female cells expressing h-2dd alloantigens. seventeen out of ...197991527
morphochemic studies on histamine and monoaminooxidase in the liver and studies on histaminolytic activity of guinea pig plasma in the course of sensitization with heterogenic protein. 197991564
rat liver cytosolic azoreductase. electron transport properties and the mechanism of dicumarol inhibition of the purified enzyme. 197991608
further studies of the antigenic properties of h3n2 strains of influenza a isolated from swine in south east asia.h3n2 strains of influenza a isolated from swine in hong kong were compared with human strains of h3n2 influenza a variants in reciprocal hi tests using ferret sera. one isolate from swine was indistinguishable from a/hong kong/68, one set of viruses isolated in 1976 and 1977 was most related to a/hong kong/68 but was not identical to it, two isolates from 1976 were 'bridging strains' that cross-reacted equally with the contemporary variants a/victoria/3/75 and a/texas/1/77, similarly to a small ...197991663
decreased axonal flux of retrogradely transported glycoproteins in early experimental diabetes. 197991664
[method of obtaining a y. pestis fraction containing heterogenetic antigens]. 197991715
decline in hypernatraemia. 197991864
a canine model for the study of allergic asthma and suppression of hapten-specific ige antibody response. 197991961
[the effect of cerebral perfusion pressure in cerebral infarction after occlusion of middle cerebral artery in dog (author's transl)]. 197991991
serological cross-reactions of leptospiral lipopolysaccharide (f4) antigen.the serological specificity of leptospiral lipopolysaccharide (f4) antigen was examined by the technique of passive haemagglutination. f4 extracted from leptospiral serovars representative of several different serogroups showed wide cross reaction between serovars, including numerous one-way (non-reciprocal) reactions. the pattern of cross reaction was different to that of the standard leptospiral classification scheme.197992115
viability assessment by dye exclusion. a fluorescent method for fungal cells.a new fluorescent staining technique for fungi utilizes the differential affinity of dead cells for rhodamine b. a mounting medium has been devised that includes the dye. this medium allows direct processing of cells from a broth and provides optimal conditions for fluorescence intensity. comparison with the standard methylene blue exclusion test as applied to the candida albicans yeast phase indicates similar specificity. various pseudohyphae and several fungi also exhibited selective uptake of ...197992216
[orthosympathetic stimulation after hypercapnia in the rat].inhalation of a gas mixture : co2, 25% -- o2, 37.5% -- n2, 37.5%, provokes regularly in the light anaesthetized rat, an increase of catecholaminemia of central origin, associated with systemic arterial hypertension.197992225
processing of 19 s thyroglobulin in vitro [proceedings]. 197992247
shielding of phospholipid vesicles from phospholipase c hydrolysis by alpha-lactalbumin adsorption [proceedings]. 197992261
the antagonistic effect of dexamethasone and insulin on alpha-fetoprotein secretion by cultured h4-ii-e-c3 cells derived from the reuber h-35 hepatoma. 197992316
isolation of plasma-membrane components from cultured human pancreatic cancer cells by immuno-affinity chromatography of anti-beta 2m sepharose 6mb.human pancreatic exocrine adenocarcinoma cells established in tissue culture expressed both hla and beta 2-microglobulin (beta 2m). plasma-membrane components of this pancreatic cancer cell line were purified from plasma membrane fractions enriched by sucrose density-gradient centrifugation, using immunoaffinity chromatography on immobilized anti-human beta 2m antibody. both rabbit and mouse monoclonal anti-beta 2m igg were used, with a 20--25-fold overall purification of 5'-nucleotidase. the me ...197992330
[5-azacytidine and new formation of purine nucleotides, rna, proteins, and lipids in the cerebral cortex of guinea pigs (author's transl)]. 197992352
[clinical effects of chlormadinone acetate (cma) on human benign prostatic hypertrophy--comparative study with hexestrol by a double blind trial (author's transl)]. 197992377
populational polymorphisms in silver staining of nucleolus organizer regions (nors) in human acrocentric chromosomes.the ag stainability of the nucleolus organizer region (nor) was studied in the acrocentric chromosomes identified by q banding of cultured lymphocytes in 41 karyotypically normal persons (33 males and 8 females) originating from southeast estonia. the data obtained are compared with those established earlier for a combined vienna-ulm population of 51 karyotypically normal persons (see mikelsaar et al., 1977a). significant differences between the two populations in the frequency and patterns of a ...197992452
nonspecific light loss and intrinsic dna variation problems associated with feulgen dna cytophotometry.nonspecific light loss by the cell-wall-plus-cytoplasm (cwc) can cause a 50% increase in feulgen absorption units in peanut root-tip nuclei as determined by scanning at 450 nm, whereas this phenomenon is not evident with chicken erythrocytes. a two wavelength scanning method of subtracting nonspecific 450 nm absorption from 550 nm feulgen absorption values eliminated the nonspecific light loss in cwc, however, the two wavelength scanning method is time consuming and somewhat impractical with a r ...197992496
ia determinants on human t-cell subsets defined by monoclonal antibody. activation stimuli required for expression.the nature of ia antigens which appear on human t cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with the ia antigen framework. t cells were purified using monoclonal antibodies directed either at the entire t-cell population (okt3) or the t-cell inducer subset (okt4). by indirect immunofluorescence, it was shown that the human t-cell population contains no detectable ia+ cells in the resting state. in contrast, in exces ...197992523
biologically active polycycloalkanes. 6. antiviral 1-tricyclo[ 2,5]undecyl derivatives.functionalization reactions via cationic intermediates of tricyclo[ 2,5]undecane (2) were investigated to prepare derivatives with potential antiviral activities. bromination of 2 took place regiospecifically at c-1, and the resulted bromide 5 was converted into the hydroxide 9, the carboxylic acid 12, and the amine 22, from which were synthesized a variety of secondary derivatives, including homologous esters 10 and 20, amides 14 and 19, carbamates 24, and ureas 17 and 25. the hydroxide ...197992566
[method of differentiating k. ozaenae and k. rhinoscleromatis by means of a biotest]. 197992603
olivocerebellar projections in the cat studied by means of anterograde axonal transport of labelled amino acids as tracers. 197992769
intracellular ph transients induced by chloride substitution in cardiac purkyne fibres [proceedings]. 197992940
isolation and cell culture propagation of rotaviruses from turkeys and chickens.rotaviruses were detected by electron microscopy in the faeces of turkey poults and broiler chickens with diarrhoea. apparently symptomless infection was also observed in broilers. the avian rotaviruses could not be isolated in cell cultures by conventional techniques, but were adapted to serial growth in chick cell cultures following trypsin treatment of the virus and the cells. immunofluorescence studies showed that the avian and mammalian rotaviruses are antigenically related. antibodies to r ...197992979
selective aromatic-hydrophobic binding and fractionation of immunoglobulin by means of phenyl-(ch2)n-nh-substituted agaroses. 197992988
presence of human milk particle--specific antigens in malignant human breast tumor tissues. 197993088
quantitative succinate-dehydrogenase histochemistry. i. a methodological study on mammalian and fish enzyme histochemistry formazan production can be used as a measure for oxidative enzyme activity. the formazan deposits can be measured quantitatively per cell with a scanning and integrating microspectrophotometer. optimal conditions are described for the estimation of histochemical succinate dehydrogenase activity in sections of fish bodymusculature and mouse soleus and plantaris muscle. it is shown that when proper measuring conditions are chosen a ditetrazolium salt (tnbt) can be used in ...197993099
immunological relationships among ovine glycoprotein antiserum to partially purified ovine fsh (but essentially free of lh) bound 125i-labelled ovine lh or bovine tsh. the antibody was directed exclusively against determinants in the alpha subunit. in a radioimmunoassay, only the intact ovine and bovine hormones and their alpha subunits were reactive; the hormone specific beta subunits exhibited no cross-reaction. the antibody directed against the alpha subunit was highly dependent on conformation. human lh, fsh, tsh or their alpha subunits did ...197993139
specificity of serovar-specific main antigens of leptospiras shown by the inhibition of leptospiral microscopic agglutination. 197993155
[determination of human blood protein phenotypes]. 197993164
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