| comparison of interferon action in interferon resistant and sensitive l1210 cells. | translation inhibition, leu-trna aminoacylation and double-stranded rna and atp dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse l 1210 r and l 1210 s cells. no differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67k dalton phosphoprotein fraction in interferon-treated l 1210 s cell-free extracts. in non-responding cell-free lysates, the lack of sti ... | 1978 | 212514 |
| [free radicals in normal and pathological conditions of the body]. | | 1978 | 212725 |
| immunological studies on cytochrome c oxidase: arrangements of protein subunits in the solubilized and membrane-bound enzyme. | seven protein subunits of cytochrome c oxidase from bovine heart were isolated by gel filtration in the presence of sodium dodecyl sulphate (subunits i, ii and iii) and guanidine hydrochloride (subunits v, vi and vii), and ion-exchange chromatography in 6 m urea (subunit iv) after the enzyme had been dissociated in 6 m guanidine hydrochloride. when analysed by highly cross-linked sodium dodecyl sulphate/polyacrylamide gel electrophoresis in the presence of urea, the apparent molecular weights we ... | 1978 | 213267 |
| amino acid sequence of a peptide containing an essential cysteine residue of escherichia coli gmp synthetase. | the amino acid sequence of a 51-residue tryptic peptide of citraconylated [1-14c]carboxamidomethyl-labeled escherichia coli gmp synthetase was determined by sequenator analyses of the intact peptide and fragments obtained by cleavage of the peptide with cyanogen bromide, trypsin, and staphylcoccus aureus strain v8 protease. the cysteine residue of this peptide fragment is essential for glutamine-dependent gmp synthesis activity and is implicated in formation of a hypothetical covalent glutamyl-e ... | 1978 | 213434 |
| [an autopsy case of acute myeloblastic leukemia with persistent high anti ebv-antibody titer (author's transl)]. | | 1978 | 213625 |
| mechanism of action of amantadine on neuromuscular transmission. | | 1978 | 213703 |
| studies on the biosynthesis of cyclitols, xxxvii. on mechanism and function of schiff's base formation as an intermediary reaction step of myo-inositol-1-phosphate synthase from rat testicles. | it could be shown that the formation of a schiff's base by myo-inositol-1-phosphate synthase of rat testicles occurs by binding the aldehyde group of the open form of its substrate, d-glucose 6-phosphate, to a lysyl residue of one or both smaller subunits of the enzyme. the participation of the schiff's base formation in the catalytic process is supported by the observations that (a) no schiff's base is formed if nad is removed from the enzyme, and (b) in the presence of nad, the dehydrogenation ... | 1978 | 214386 |
| co-induction of beta-galactosidase and the lactose-p-enolpyruvate phosphotransferase system in streptococcus salivarius and streptococcus mutans. | the addition of lactose, galactose, or isopropyl-beta-d-thiogalactoside (iptg) to glucose-grown cells of streptococcus salivarius 25975 resulted in the co-induction of both the lactose-p-enolpyruvate phosphotransferase system (lactose-pts) and beta-galactosidase, with the latter the predominant metabolic system. with various strains of streptococcus mutans and streptococcus sanguis 10556, on the other hand, the lactose-pts was the major metabolic pathway with beta-galactosidase induced either to ... | 1978 | 214423 |
| mesoionic xanthine analogs as inhibitors of cyclic amp phosphodiesterase. | several derivatives of two mesoionic xanthine analogs, mesoionic thiazolo[3,2-alpha]pyrimidine-5,7-diones and mesoionic 1,3,4-thiadiazolo[3,2-alpha]pyrimidine-5,7-diones, were synthesized and evaluated as inhibitors of cyclic amp phosphodiesterase. a significant number of these compounds demonstrated theophylline-like activity. | 1978 | 214541 |
| formulation and evaluation of ethiodized oil emulsion for intravenous hepatography. | a study was conducted to prepare and evaluate an ethiodized oil emulsion for intravenous administration that would selectively opacify the liver. several formulations with differing globule size were prepared and compared for their in vivo activity (degree of liver opacification obtained) and stability. results of studies conducted in rabbits and monkeys revealed that the oil globules that concentrate most in liver were 2.0--3.0 micrometer in diameter. the formulation of choice was stable for 6 ... | 1979 | 214542 |
| survey for antibodies to bovine leukemia virus in dairy and beef cattle in japan. | | 1978 | 214603 |
| hepatosplenic myelosis in naturally occurring mouse hepatitis virus infection in the nude mouse. | | 1978 | 214604 |
| inhibition of the binding of low-density lipoprotein to its cell surface receptor in human fibroblasts by positively charged proteins. | a group of proteins and polyamino acids with positively charged domains were shown to inhibit the binding of 125i-ldl to its receptor on the surface of human fibroblasts. the list of inhibitory proteins included platelet factor 4 (which has a cluster of lysine residues at its carboxyl terminus), two lysine-rich histones, poly-l-lysines of chain length greater than 4, and protamine. these proteins were effective in the concentration range of 5--10 microgram/ml. two other positively charged protei ... | 1978 | 214639 |
| nonspecific inhibitors of coronavirus oc43 haemagglutination in human sera. | antibodies against the human coronavirus oc43 in human sera were measured by haemagglutination inhibition (hi), complement fixation (cf), and radial diffusion-haemolysis in gel (hig) techniques. the apparent hi titres in a fraction of sera with no antibodies detectable by the two other methods were found to be reduced considerably after treating the sera with phospholipase c (plc). the plc treatment also reduced the apparent hi titres in some sera containing variable amounts of cf and/or hig ant ... | 1978 | 214680 |
| temperature-sensitive mutants of herpes simplex virus type 1 defective in transcriptional and post-transcriptional functions required for viral dna synthesis. | | 1978 | 214940 |
| [pathogenesis of brain lesions in acute viral respiratory infections (review)]. | | 1978 | 214977 |
| attenuated dynamic responses of primary endings of muscle spindles: a basis for depressed tendon responses in acrylamide neuropathy. | depressed or lost tendon reflexes commonly observed in patients with peripheral neuropathies may result partly from attenuation of the dynamic discharge from the primary endings of muscle spindles. this possibility was investigated in cats with an experimental neuropathy induced with acrylamide (30 mg/kg/day intramuscularly). achilles tendon reflexes and the dynamic discharge from primary muscle spindles were evaluated after five or ten injections of acrylamide. after five injections the animals ... | 1978 | 215077 |
| evidence for antiferromagnetic exchange coupling in the tetranuclear high potential iron-sulfur protein of rhodopseudomonas gelatinosa. | | 1978 | 215143 |
| nitrogenase x: mössbauer and epr studies on reversibly oxidized mofe protein from azotobacter vinelandii op. nature of the iron centers. | under anaerobic conditions the molybdenum-iron protein (mofe protein) from azotobacter vinelandii can be reversibly oxidized with thionine. electron paramagnetic resonance studies reveal that the oxidation proceeds in two distinct phases: the mofe protein can be oxidized by four electrons without loss of the epr signal from the s = 3/2 cofactor centers. a second oxidation step, involving two electrons, leads to the disappearance of the cofactor epr signal. in order to correlate the events during ... | 1978 | 215215 |
| ribonucleic acid synthesis in rat liver during fatty acid stimulated secretion of very low density lipoproteins. | production of very low density lipoproteins by the liver depends on the cellular availability of fatty acids. it is stimulated by the uptake of free fatty acids from the plasma and by increased lipogenesis and is inhibited by actinomycin d, suggesting that rna synthesis is involved in the regulation of apolipoprotein synthesis. this hypothesis has been investigated in rats in vivo and in isolated perfused livers with and without stimulation by fatty acid overload: [14c] orotate incorporation in ... | 1978 | 215235 |
| ongoing activity in severed nerves: source and variation with time. | | 1978 | 215270 |
| the free radical in ribonucleotide reductase from e. coli. | protein b2, one of the subunits of ribonucleotide reductase from escherichia coli, contains a stable free radical. it is characterized by a doublet e.p.r. signal centered around g = 2.0047 and a sharp peak at 410 nm in the optical spectrum. the radical has been assigned to a tyrosyl residue in the protein with its spin density delocalized over the aromatic ring. protein b2 also contains two antiferromagnetically-coupled high-spin iron(iii) atoms, which stabilize the free radical. protein b1, the ... | 1977 | 215392 |
| penetration of cultured mouse fibroblasts (l cells) by rickettsia prowazeki. | the association of rickettsia prowazeki with l cells was examined by using a novel radioactive assay in which [alpha-(32)p]atp-labeled rickettsiae were incubated with l-cell monolayers. rickettsial association with the monolayer involved adherence and internalization steps that could be experimentally distinguished. since r. prowazeki but not l cells possess an atp-adp obligate exchange transport system, addition of excess unlabeled atp resulted in exchange of the labeled atp from external, adhe ... | 1978 | 215542 |
| characterization of human tk- cell lines transformed to a tk+ phenotype by herpes simplex virus type 2 dna. | human tk- cells carrying the hsv-2 tk gene as a result of transformation with virus dna express a tk activity of virus origin and maintain the tk+ phenotype when grown in hat medium. under non-selective conditions, however, reversion to a tk- phenotype occurs with a significant frequency characteristic of each transformed line. once reversion has occurred the tk- phenotype appears to be stable, since only very rare instances of tk- to tk+ reversion have been observed. tk- revertants were suscept ... | 1979 | 215706 |
| evidence for the cell surface locus of presynaptic purine nucleotide receptors in the guinea-pig ileum. | | 1978 | 215742 |
| synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a sv40 beta-globin recombinant genome. | rabbit beta-globin complementary dna (cdna) has been inserted into sv40 dna in place of the gene coding for the virus' major capsid protein, vp1. the recombinant genome, svgt5-rabetag, multiplies efficiently in cv1 monkey kidney cell cultures and is transcribed to yield cytoplasmic, polyadenylated hybrid mrnas containing the beta-globin coding sequence. cells propagating svgt5-rabetag produce substantial quantities of rabbit beta-globin polypeptide. | 1979 | 215915 |
| effects of haemophilus influenzae vaccination on the (para-)sympathic-cyclic nucleotide-histamine axis in rats. | to determine whether haemophilus influenzae could be a factor in human atopy its effects were studied on the (para-)sympathic cyclic nucleotide-histamine axis in rats. haemophilus influenzae vaccination induced changes in the cholinergic system compatible with higher cyclic gmp levels and enhanced histamine release. the authors suggest an involvement of the cholinergic system in haemophilus influenzae vaccination effects. | 1979 | 216288 |
| search for type-c oncornavirus-related genetic information in tissues from patients with systemic lupus erythematosus. | single-stranded 3h-dna probes complementary to the rna of rauscher murine leukemia virus and of simian sarcoma virus were prepared using techniques that permitted complete transcription of the viral genome of each virus. these probes were used in dna-dna hybridization studies with the cellular dna from uncultured specimens of spleens and placentas of patients with systemic lupus erythematosus (sle). no proviral dna sequences related to these viruses were detected in these tissues. the results pr ... | 1978 | 216374 |
| electrogenic events in the ubiquinone-cytochrome b/c2 oxidoreductase of rhodopseudomonas sphaeroides. | the reductant of ferricytochrome c2 in rhodopseudomonas sphaeroides is a component, z, which has an equilibrium oxidation-reduction reaction involving two electrons and two protons with a midpoint potential of 155 mv at ph 7. under energy coupled conditions, the reduction of ferricytochrome c2 by zh2 is obligatorily coupled to an apparently electrogenic reaction which is monitored by a red shift of the endogeneous carotenoids. both ferricytochrome c2 reduction and the associated carotenoid bands ... | 1979 | 216398 |
| coupling of glucagon receptor to adenylyl cyclase. requirement of a receptor-related guanyl nucleotide binding site for coupling of receptor to the enzyme. | effects of glucagon and guanyl nucleotides on the rat liver plasma membrane adenylyl cyclase were studied. it was established that: 1) glucagon stimulates the fully guanyl-5'-yl imidodiphosphate (gmp-p(nh)p)-activated enzyme between 20 and 70%, provided a guanyl nucleotide is present in the assay; 2) glucagon has no effect on adenylyl cyclase activity in membranes activated fully by gmp-p(nh)p and then washed free of nucleotides. it is concluded that occupancy of the guanyl nucleotide binding si ... | 1979 | 216687 |
| bactericidal activity of a superoxide anion-generating system. a model for the polymorphonuclear leukocyte. | the acetaldehyde-xanthine oxidase system in the presence and absence of myeloperoxidase (mpo) and chloride has been employed as a model of the oxygen-dependent antimicrobial systems of the pmn. the unsupplemented xanthine oxidase system was bactericidal at relatively high acetaldehyde concentrations. the bactericidal activity was inhibited by superoxide dismutase (sod), catalase, the hydroxyl radical (oh.) scavengers, mannitol and benzoate, the singlet oxygen (1o2) quenchers, azide, histidine, a ... | 1979 | 216766 |
| [a simple technique to test the functional capacity of human monocytes (author's transl)]. | | 1978 | 217112 |
| s-iga cholera toxin and rotavirus antibody in human colostrum. | s-iga antibodies against cholera toxin and rotavirus were assayed in 43 colostral samples by means of an enzyme-linked immunosorbent assay (elisa). all specimens contained specific s-iga antibodies against both antigens. significant antibody titres to the antigens were demonstrated in almost all colostral samples. | 1979 | 217230 |
| development and regression of exercise-induced cardiac hypertrophy in rats. | adult female rats were exercised by daily swimming. all the increase in heart weight induced by the exercise occurred within 14 days and averaged 30%. the half times of the increases in heart weight and total protein content were about 4.5 days, whereas that of cytochrome c, which was used as a mitochondrial marker, was 6.5 days. the total amounts of dna and of hydroxyproline in the heart, which were used to evaluate the degree of connective tissue hyperplasia, increased only slightly (8% and 10 ... | 1979 | 217279 |
| effect of beta-lapachone on superoxide anion and hydrogen peroxide production in trypanosoma cruzi. | addition of beta-lapachone, an o-naphthoquinone endowed with trypanocidal properties to respiring trypanosoma cruzi epimastigotes induced the release of o2- and h2o2 from the whole cells to the suspending medium. the same beta-lapachone concentration (4 micron) that released h2o2 at maximal rate completely inhibited t. cruzi growth in a liquid medium. the position isomer, alpha-lapachone, did not stimulate o2- and h2o2 release, and did not inhibit epimastigote growth. beta-lapachone was able to ... | 1978 | 217340 |
| role of peripheral side chains of vitamin b12 coenzymes in the reaction catalyzed by dioldehydrase. | | 1979 | 217415 |
| effect of thyrotoxicosis on gluconeogenesis from alanine in the perfused rat liver. | the influence and mechanism of action of t4 treatment on hepatic gluconeogenesis from alanine was studied in isolated rat livers. conversion of alanine into glucose was increased markedly in livers of thyrotoxic rats compared to that in normal rats. estimation of metabolic intermediates of the gluconeogenic pathway showed that t4 treatment produced forward cross-over between pyruvate and phosphoenolpyruvate, which suggests that this point is a control site. alanine transport into the liver cells ... | 1978 | 217582 |
| entamoeba histolytica: genetic control of susceptibility in chicken eggs. | | 1979 | 217709 |
| reconstitution of vitamin d3 25-hydroxylase activity with a cytochrome p-450 preparation from rat liver mitochondria. | | 1979 | 217740 |
| comparative studies on the influence of hormones on metabolite compartmentation in isolated liver cells during gluconeogenesis from lactate. | | 1978 | 217770 |
| effect of intoxication of pregnant rats with trichlorphon on the activities of some brain enzymes in the progeny during postnatal development. | | 1979 | 218149 |
| cell surface changes associated with mutation of visna virus in antibody-treated cell cultures. | | 1979 | 218349 |
| [effect of radioprotectors on camp-dependent protein phosphorylation]. | the capacity of radioprotectors (serotonin, aminoethylisothiuronium) to stimulate in radioprotective doses camp-dependent protein phosphorylation of the cytozol and nuclei of the mouse liver and of the spleen cytozol was shown in vivo. radioprotectors were incapable of modulating in vitro the activity of protein kinases and the stimulating effect of camp. the data presented and previous investigations suggest that the activation of camp-dependent phosphorylation by radioprotectors was due to the ... | 1979 | 218656 |
| an inherited anomaly of human eosinophils and basophils. | inclusions staining gray or blue-gray in romanowsky treated films were found in eosinophils and basophils in a family as an autosomal dominant anomaly without overt clinical effects. associated with the inclusions were distinctive crystals which were different from the crystalloids of eosinophil granules and from charcot-leyden crystals. the restriction of the anomaly to eosinophils and basophils indicates that these are related more closely to each other than to neutrophils and mast cells, and ... | 1978 | 218657 |
| cyclic nucleotides, cyclic nucleotide phosphodiesterase, and development in myxococcus xanthus. | exogenous cyclic nucleotide phosphodiesterase (pd) accelerated fruiting body (fb) formation and increased territory size of aggregates in myxococcus xanthus. both guanosine 3'5'-monophosphate (cgmp) and guanosine 5'-monophosphate (gmp) were antagonistic to the pd effect. adenosine 3'5'-monophosphate (camp) increases fb numbers twofold in the absence but not in the presence of pd. pd induction is not affected by methionine or isoleucine, which inhibit, or by threonine, which stimulates, fb format ... | 1978 | 218712 |
| characterization of an epstein-barr virus-induced dna polymerase. | the addition of iododeoxyuridine to p3hr-i cell cultures led to a large increase in both epstein-barr virus (ebv)-induced dna polymerase activity and early antigen-positive cells. this ebv-induced dna polymerase was separated from the cellular alpha- and beta-polymerases by sequential column chromatography on sepharose 6b, deae-cellulose, and phosphocellulose, resulting in partial purification of about 320-fold. the partially purified-ebv dna polymerase could be distinguished from the cellular d ... | 1979 | 219209 |
| tryptic peptide analysis of gag gene proteins of endogenous mouse type c viruses. | tryptic digests of the internal proteins p30, p15, p12, and p10 of mouse xenotropic, ecotropic, and amphotropic type c viruses were subjected to cation-exchange chromatography. analysis of these maps revealed that the p30 proteins from representative isolates of all three viral subgroups were distinguishable. the p15 proteins were all unique. the p12 proteins of nzb xenotropic and wild-mouse amphotropic viruses were not identical and yielded peptide maps remarkably different from that of the eco ... | 1979 | 219210 |
| the resistance of g mouse fibroblasts to murine leukemia virus infection. | in vitro resistance of g mouse cells was not expressed in the initial cycle of mulv infection but in the propagation of virus to the surrounding cells. | 1978 | 219276 |
| [blood transfusion and viral diseases. recent acquisitions concerning viral hepatitis viruses, cytomegaloviruses and epstein-barr virus]. | in recent years, an increasingly clear picture has been formed of the virus-induced syndromes that may follow a blood transfusion or the use of blood derivatives. up to about 10 years ago, post-infusion infection was predominantly due to serum hepatitis. blumberg's discovery of hbsag (formerly known as australia antigen) has made it possible to check and prevent viral hepatitis, type b, and to recognise such distinct forms as the mononucleosis-like syndrome caused by cytomegalic virus, infectiou ... | 1979 | 219399 |
| cytomegalovirus infection in cancer patients. | | 1978 | 219406 |
| [indirect hemagglutination reaction in viral (transmissible) gastroenteritis in swine]. | | 1979 | 219585 |
| inhibition of rat liver glucose 6-phosphatase by n-alkylmaleimides [proceedings]. | | 1979 | 220112 |
| effects of the aflatoxins on atpase activities in mouse and rat liver. | the effects of the aflatoxins on atpase activities in mouse and rat tissues were investigated in vitro. the hepatic oligomycin-sensitive (o.s.) mg++ atpase was inhibited significantly. the order of inhibition was g1 greater than b1 greater than g2 greater than b2. mouse o.s. mg++ atpase was more sensitive than the corresponding rat enzyme. the oligomycin-insensitive (o.i.) mg++ atpase activities in rat and mouse liver were not altered. although aflatoxins g1 and b1 were more potent inhibitors of ... | 1979 | 220299 |
| extraction of soluble antigens of epstein-barr virus, herpesvirus salmirl, and herpesvirus ateles with the use of glycine. | for extraction of soluble antigen from cells infected with epstein-barr virus, herpesvirus salmirl, and h. ateles, 0.1 m glycine (ph 9.5) was used. this method yielded increased amounts of the antigen containing much less cell debris. lymphoblastoid cells infected with epstein-barr virus could maintain up to 50% viability after the extraction procedure. these cells could be used again after an appropriate interval in culture. the usefulness of this technique is discussed. | 1979 | 220447 |
| influence of intramuscular or intravenous injection of cholecalciferol on serum calcium, phosphorus and magnesium levels in the bovine. | | 1979 | 221431 |
| [effect of potassium orotate and guanine on calcium uptake in small intestine of chickens with different amounts of vitamin d3]. | potassium orotate and guanine are established to accelerate the duodenum physiological response of chickens with experimental rachitis to administration of vitamin d3 in the in vivo experiments. they stumulate the uptake and transport of calcium, increase the content of calcium-binding protein and affect the activity of alkaline phosphatase. guanine is a more effective stimulator as compared to orotate. a rise in the calcium uptake in the duodenum of the chicken which were given guanine started ... | 1979 | 222028 |
| role of albumin in hormone-stimulated lipolysis. | | 1979 | 222292 |
| hydrogenase activity in the dry state: isotope exchange and reversible oxidoreduction of cytochrome c3. | hydrogenase (hydrogen:ferricytochrome c3 oxidoreductase, ec 1.12.2.1) catalyzes three types of reactions, i.e., (1) conversion between hydrogen modifications, para-h2 and ortho-h2, (2) exchange reaction between hydrogen isotopes, and (3) reversible oxidoreduction of an electron carrier with h2 and protons. we observed that purified desulfovibrio hydrogenase in the dry state could catalyze not only the conversion and exchange reactions (yagi, t., tsuda, m., mori, y. and inokuchi, h. (1969) j. am. ... | 1979 | 222328 |
| the ebv-carrying, beta2m/hla deficient burkitt lymphoma line daudi is sensitive to ebv-specific killer t-cells of mononucleosis patients. | the beta2-microglobulin/hla deficient burkitt lymphoma line daudi was tested for sensitivity to ebv-specific cytotoxicity mediated by natural killer (nk)-depleted t-cells from acute mononucleosis patients. while the daudi line was not as sensitive as the reference ebv-genome-positive target line, it was clearly sensitive in the majority of cases. this would speak against a major role of syngeneic restriction in this system. | 1979 | 222436 |
| stereospecific effects of opiate antagonists on superficial and deep nociception and on motor activity suggest involvement of endorphins on different opioid receptors. | | 1979 | 222987 |
| detection of a precursor of bovine leukemia virus structural proteins in purified virions. | gel filtration chromatography of disrupted bovine leukemia virus (blv) resulted in the isolation of the 25,000 dalton major virion internal protein and two previously uncharacterized virion proteins of molecular weight 65,000 and 12,000 respectively. the 65,000 dalton protein does not bind to concanavalin a and its antigenicity is ether-resistant. therefore, this polypeptide is different from the previously described glycoprotein associated with blv. radiommunoprecipitation and competitive radio ... | 1978 | 224756 |
| the nucleotide sequence and genome organization of the polyoma early region: extensive nucleotide and amino acid homology with sv40. | | 1979 | 225042 |
| mecillinam-ampicillin synergism in experimental enterobacteriaceae meningitis. | the in vitro activities of mecillinam, a new beta-amidinopenicillin, and ampicillin, alone and in combination, against an escherichia coli strain and a klebsiella pneumoniae strain were compared, and these results were correlated with their respective activities in vivo in experimental meningitis. the mecillinam-ampicillin combination was synergistic in vitro against both strains when tested by a modified checkerboard technique (bacteriostatic synergy). however when quantitative bactericidal syn ... | 1979 | 228591 |
| adenosine 3',5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate phosphodiesterase activities in 1,2-dimethylhydrazine induced colon adenocarcinoma. | the intracellular concentrations of adenosine 3',5'-cyclic monophosphate (camp) and guanosine 3',5'-cyclic monophosphate (cgmp) in 1,2-dimethylhydrazine (dmh) induced rat colon tumors were previously reported to be one-half and twice, respectively, that measured in normal rat colon tissue. the results of this present study indicate that the total amount of cyclic nucleotide hydrolysis by the phosphodiesterase enzymes (pde) could account for the lower camp and elevated cgmp noted in cancer tissue ... | 1979 | 228847 |
| free radicals in cancer. | it has been hypothesized that free radicals play a significant role in cancer. a historical review indicates a series of rises and falls in the acceptance of this hypothesis and it remains controversial. the strongest evidence for a critical role of free radicals in cancer is based on electron spin resonance (e.s.r.) data from lyophilized (freeze-dried) tumours. recent results indicate that such data are artifactual in the sense that the observed signals are not directly related to free radicals ... | 1978 | 228915 |
| incidence of bluetongue virus precipitating antibodies in sera of some domestic animals in the sudan. | to determine the presence and prevalence of bluetongue (bt) infection in a variety of domestic animal species in different geographical regions of the sudan, a serological study using the agar gel precipitation technique was initiated. a total of 2142 serum samples were examined. of the numbers tested approximately 28% of sheep, 11.2% of goats, 8% of cattle and 4.9% of camels were positive for group-specific antibodies to bt virus antigen, indicating previous exposure to bt infection. none of th ... | 1979 | 229163 |
| lethal effect of complement and lysozyme on polymyxin-treated, serum-resistant gram-negative bacilli. | when genetically serum-resistant escherichia coli, klebsiella pneumoniae, and citrobacter freundii, but not pseudomonas aeruginosa or proteus mirabilis, were exposed to polymyxin b, they became susceptible to the bactericidal action of normal human and rabbit sera. in constrast, beta-lactam and aminoglycoside antibiotics did not render any serum-resistant bacteria serum-sensitive. synergy between polymyxin b and the serum bactericidal system could be demonstrated by the addition of polymyxin b t ... | 1979 | 229175 |
| properties of human epithelioid cells established in vitro by a herpesvirus [ibrv(hmc)] isolated from cytomegalovirus-transformed human cells. | infectious bovine rhinotracheitis virus [ibrv(hmc)], a double-enveloped herpesvirus, was isolated from human embryo lung fibroblasts transformed by cytomegalovirus (cmv). this agent was identified as an ibrv strain that was antigenically related to human cmv. inoculation of a primary human kidney cancer cell culture with ibrv(hmc) resulted in persistent infection and subsequent establishment of a cell line [ibrv(hmc)hkc-1]. virus-related nuclear, cytoplasmic, and cell membrane antigens were dete ... | 1979 | 229291 |
| [responses of the vibrissal projection zone of the cat somatosensory projection zone to afferent activation]. | responses of 375 neurons of si cortex region in the vibrissae projection zone were recorded in unanesthetized cats: responses to electrical stimulation of infraorbital nerve and mechanical stimulation of vibrissae were studied. nerve and vibrissae stimulation evoked complex synaptic potentials (short epsps followed by ipsps) in most neurons primary ipsps were found in other units. the corresponding changes of impulse activity could be recorded in many neurons extracellularly. initial inhibition ... | 1979 | 229432 |
| methotrexate with citrovorum factor rescue for nonmetastatic gestational trophoblastic neoplasms. | fifty-one patients with nonmetastatic gestational trophoblastic neoplasms (nmgtn) were treated with either 4 or 6 mg/kg methotrexate (mtx) and citrovorum factor (cf) rescue to determine if the higher dosage could reduce the number of courses of chemotherapy required to achieve remission. thirty-six of 41 patients treated with 4 mg/kg mtx achieved complete remission with 1 course of chemotherapy. increasing the initial dose of mtx to 6 mg/kg in 10 patients did not reduce the need for subsequent c ... | 1979 | 229448 |
| a clinico-electrophysiological study of three cases of post-infective polyneuritis with severe involvement of cranial nerves. | | 1979 | 229701 |
| interaction of cytochrome c with the phosphorprotein phosvitin. | candida krusei cytochrome c forms a molecular complex with phosphorprotein phosvitin in weakly alkaline solution of low ionic strength. at most, about 22 molecules of cytochrome c bind to a phosvitin molecule. the complex at the binding ratio below about 11 (half of the maximum ratio) as a much higher binding strength. several lines of evidence indicate that the marked difference in the binding strength is due to the difference in negative charges on phosvitin molecule concerned in the binding o ... | 1979 | 229914 |
| pleiotropic mutations within two yeast mitochondrial cytochrome genes block mrna processing. | the mrnas from two yeast mitochondrial genes cob-box (cytochrome b) and oxi-3 (cytochrome oxidase 40,000 dalton subunit) are processed from large (7-10 kb) precursors. certain mutations in each gene block the maturation of the rnas from both genes at a variety of specific steps. the pleiotropic cytochrome b mutants seem to lack a functional trans-acting rna required for the processing of both messengers. in contrast, the oxi-3 mutants may act by producing an activity that inhibits specific steps ... | 1979 | 229970 |
| investigations into the klebsiella aerogenes pentitol operons using specialised transducing phages lambdaprbt and lambdaprbt dal. | | 1979 | 230352 |
| specificity of attachment of certain enterobacteriaceae to mammalian cells. | the specificity of adherence of various enterobacteriaceae to different mammalian cells was studied in vitro. 3h-labelled organisms of the same species isolated from various clinical sources differed significantly in their abilities to adhere to the same mammalian cells. bacteria frequently adhered better to cells derived from sites other than those analogous to their original source. bacteria did not display consistently 'high' or 'low' attachment to a variety of human and tissue-cultured cells ... | 1979 | 231084 |
| cloning of the entire region for nitrogen fixation from klebsiella pneumoniae on a multicopy plasmid vehicle in escherichia coli. | | 1979 | 232231 |
| characterization of avian reoviruses isolated from the synovia and breast blister. | reoviruses texas, s 1133, umi 203, and wvu 2937 induced swelling of the foot pad and inflammatory changes in the synovial membranes when inoculated in the foot pad of 2-week-old gnotobiotic chicks. from differences in virus neutralization as measured with geometric mean (r) value, all four viruses are subtypes of a single serotype. the cell-associated and cell-released virus growth curves were similar, with a lag phase of about 15 hours and a log phase of 15 to 21 hours. viral rna synthesis reac ... | 1979 | 232658 |
| thromboxane b2: a cardiodepressant of isolated rat hearts and inhibitor of sarcolemma na+ - k+ stimulated atpase activity. | the actions of thromboxane b2 on various parameters of cardiac performance were studied using the isolated perfused rat heart model. in concentrations from 100 pg/ml to 1 microgram/ml txb2 significantly reduced the total generated myocardial contractile force. these changes were usually associated with an increase in the coronary perfusion pressure indicating an elevated coronary vascular resitance. significant coronary pressure alterations were seen with txb2 concentrations between 1 ng/ml and ... | 1979 | 233217 |
| [clinico-bacteriological correlations in patents with chronic nonspecific diseases of the lungs]. | | 1975 | 234629 |
| 1-alkyl-2-nitroimidazol-5-yl derivatives. i. | | 1975 | 235267 |
| a purification procedure for the isolation of homogeneous preparations of bovine aorta amine oxidase and a study of its lysyl oxidase activity. | it has been reported that bovine aorta amine oxidase oxidizes lysine residues in tropoelastin to allysine (rucker, r.b. and o'dell, b.l. (1971) biochim. biophys. acta 235, 32-43). pure bovine aorta amine oxidase was isolate by deae-cellulose, hydroxylapatite, bio-gel a-1.5 m and concanavalin a-sepharose 4b chromatography. enzymatic, chromatographic and immunochemical tests disclosed that pure bovine aorta amine oxidase was not a lysyl oxidase capable of oxidizing the lysine residues of tropoelas ... | 1975 | 235299 |
| evaluation of bacterial contamination in blood processing. | in the routine deglycerolization of frozen red blood cells, a bottle of solution intended as a final wash (0.8% nacl, 0.2% dextrose) was found to be abnormally colored and slightly turbid. close inspection revealed a small crack in the base of the bottle. a pure growth of klebsiella pneumoniae was cultured. the rabbit pyrogen test on the solution was positive using 2.5 mul as was the limulus amebocyte lysate (lal) test at a dilution of 1:10-5 for endotoxin. studies of the growth of this organism ... | 1975 | 236609 |
| molecular biology of nitrogen fixation. | | 1975 | 238283 |
| [influence of co2 and ph on the attachment response of the cercaria of diplostomum spathaceum (trematoda) (author's transl)]. | 1. the attachment of the cercaria to artificial substrates (offered via dialyzing membranes) in definite media was investigated under conditions of variable ph and [co2]. 2. a decrease of the ph of the substrate releases only attachments in co2 containing media and consequently acts via co2 systems of the medium. 3. as effective components of co2 systems, dissolved co2 + h2co3 are confirmed. 4. the sensitivity of the reaction on gradients of the co2 partial pressure (in solution) could be establ ... | 1975 | 238346 |
| brain carbonic acid acidosis after acetazolamide. | in cats in barbiturate anesthesia extracellular ph and potassium were continously recorded from brian cortex by implanted microelectrodes. implantation of the electrodes preserved the low permeability of the blood-brain-barrier to hco3-minus and h+ions as indicated by the development of brain acidosis by i.v. injection of hco3-minus. acetazolamide (25 mg/kg) i.v. was followed by a marked brain acidosis which after 10 min had progressed to a drop in ph of 0.203 plus or minus 0.046 (x bar plus or ... | 1975 | 238362 |
| transsynaptic enzyme induction. | | 1975 | 238378 |
| in vitro penetration of tritiated dextrans through rabbit oral mucosa. | | 1975 | 238494 |
| cat haemoglobins a and b: differences in the interaction with cl minus, phosphate and co2. | | 1975 | 238526 |
| intracellular distribution of beta-galactosidases in mucosal cells from hog small intestine. | 1. intracellular distribution of three types of beta-galactosidases (beta-d-galactoside galactohydrolase ec 3.2.1.12) i.e. hetero beta-galactosidase, lactase and acid beta-galactosidase, was studied by examining the properties of subcellular fractions isolated by a systematic fractionation of mucosal cells of the small intestine of the hog. localization of hetero beta-galactosidase in cytosol could be shown. 2. localization of lactase in the brush borders was shown by analyzing the purified brus ... | 1975 | 238631 |
| catecholamine binding to plasma membrane enriched fractions of heart and skeletal muscle. | binding of [3h]epinephrine to plasma membrane enriched fractions from guinea pig heart and rabbit skeletal muscle was investigated using the micropore filtration technique. [3h]epinephrine and [3h]norepinephrine were found to be degraded rapidly in aqueous buffer at ph 7.6 and 37 degrees c. deterioration of the compounds could be prevented by low concentrations of dithiothreitol. binding of [3h]epinephrine to both membrane preparations was a slow process requiring 60 min to approach equilibrium ... | 1975 | 238726 |
| the influence of cholinoceptor activity on the mitotic rate in the crypts of lieberkühn in rat jejunum. | 1. the influence of cholinoceptor stimulation and cholinoceptor blockade on the mitotic rate in epithelial cells lining the crypts of lieberkühn in rat jejunum was studied. 2. cholinoceptor stimulation, either by injection of carbachol or by inhibition of acetylcholinesterase enzymes resulted in an increase in the mitotic rate. this increase in mitotic rate was blocked by tubocurarine, but not by atropine or hexamethonium. atropine by itself increased the mitotic rate whereas tubocurarine alone ... | 1975 | 238778 |
| a new parenteral cephalosporin. sk&f 59962: in vitro and in vivo antibacterial activity and serum levels in experimental animals. | sk&f 59962, a new parenteral cephalosporin was found to have a high order of in vitro and in vivo antibacterial activity against a broad-spectrum of clinical isolates. when tested in vitro against gram-negative organisms, sk&f 59962 was consistently more active than cefazolin and far superior to cephalothin. this new antibiotic had activity equal to that of cephalothin against gram-positive bacteria. enterobacter species were found to be susceptible to sk&f 59962. in mouse infection studies usin ... | 1975 | 238925 |
| release of adrenaline by anaphylaxis in the guinea-pig: its effect on lung lipid content. | the study was undertaken to discover whether the catecholamines released as a result of the stress and hypoxia of anaphylaxis were responsible for the concomitant loss of lipid from the lung. a method is described whereby the respiratory rate and volume and heart-rate of conscious sensitized guinea-pig were measured and the electrocardiogram recorded during anaphylaxis induced by aerosolized antigen. after 7 days or more, some animals were anaesthetized with pentobarbitone and respiration was ar ... | 1975 | 239118 |
| automated system for analytical microbiology v: calibration lines for antibiotics. | the accuracy of an automated system for the microbiological assay of antibiotics was increased by improvement attendant to connection to an on-line computer. the system was used to investigate the suitability of four forms of interpolation formulas by assaying for chlortetracycline and erythromycin. the calibration lines were prepared as point-to-point straight-line approximations and as cubic equations. cubic equations through four calibration points were preferred. since the automated system w ... | 1975 | 239199 |
| [clinical evaluation of a new oral cephalosporin preparation, cephradine dry syrup, in pediatrics (author's transl)]. | | 1975 | 239262 |
| [clinical effects of lividomycin on respiratory tract infections, primarily wet cases of bronchiectasis (author's transl)]. | patients with various respiratory infections, primarily wet cases of bronchiectasis, were treated with lividomycin (lvdm) at a dose of 1 g/day for about a week. the results obtained are as follows: 1. of the 15 patients with refractory bronchiectasis who had previously been treated with various other antibiotics, 11 patients (73.3%) responded to lvdm. although there was no patient who responded to lvdm excellently, good cures were achieved in 5 pateints and fair cures in 6 patients. 2. in bronc ... | 1975 | 239263 |
| antihypertensive effects of beta-blockers in hypertensive rats. | | 1975 | 239268 |
| the influence of alcohol and tobacco on the components of choice reaction time. | in smokers a low dose of alcohol slightly improved decision time on a choice reaction-time task and in combination with tobacco resulted in significant improvement compared with placebo conditions. in nonsmokers decision time deteriorated significantly after a moderate dose of alcohol but not in smokers. the results are attributed to the stimulant action of a smoking dose of nicotine interacting with low levels of alcohol. | 1975 | 239281 |
| boric acid tolerant vibrio cholerae: biological and physical properties. | two boric acid tolerant variants were developed by passage in boric acid containing media from a mouse virulent parent vibrio cholerae strain. the variants were stable, exhibited increased acid resistance, were less virulent to mice, but protected against challenge with the mouse virulent parent strain. | 1975 | 239521 |
| death of staphylococcus aureus in liquid whole egg near ph 8. | incubating and shaking staphylococcus aureus in liquid whole egg causes a decline in viability. during the period of agitation, the natural ph of the egg rises from about 7.2 to between 8.0 and 8.2 as a result of a loss of carbon dioxide. however, if the ph of the egg is prevented from rising, either by not shaking or by addition of a buffer, s. aureus will grow. the cause of death is traced to the presence of lysozyme of egg white. interestingly, the action of lysozyme is not attributable to it ... | 1975 | 239629 |