Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
polymyxin b in combination with meropenem against carbapenemase-producing klebsiella pneumoniae: pharmacodynamics and morphological changes.combination therapy provides a useful therapeutic approach to overcome resistance until new antibiotics become available. in this study, the pharmacodynamics, including the morphological effects, of polymyxin b (pmb) and meropenem alone and in combination against kpc-producing klebsiella pneumoniae clinical isolates was examined. ten clinical isolates were obtained from patients undergoing treatment for mediastinitis. kpcs were identified and mics were measured using microbroth dilution. time-ki ...201728040408
the pharmacodynamics of avibactam in combination with ceftaroline or ceftazidime against β-lactamase-producing enterobacteriaceae studied in an in vitro model of infection.pharmacodynamics of β-lactamase inhibitors are an area of intense interest as new β-lactam/β-lactamase inhibitor combinations enter clinical development and clinical practice. avibactam, a non-β-lactam β-lactamase inhibitor, has been combined with ceftaroline or ceftazidime but these two combinations have not been directly compared.201728039276
rectal carriage of extended-spectrum β-lactamase- and carbapenemase-producing enterobacteriaceae among hospitalised neonates in a neonatal intensive care unit in fez, morocco.the aim of this study was to investigate the faecal carriage and molecular epidemiology of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esble) isolated from rectal samples of neonates hospitalised in a neonatal intensive care unit (nicu) of a university hospital in fez, morocco.201728039104
etiology of early onset septicemia among neonates at the university college hospital, ibadan, nigeria.neonatal septicemia remains a major cause of newborn deaths in developing countries. its burden is further compounded by the emergence of multidrug-resistant pathogens, which is related to a lack of antibiotic protocols resulting in unrestricted use of antibiotics. the absence of reliable antibiotic sensitivity testing makes the formulation of antibiotic guidelines and judicious use of antibiotics difficult. this study sought to identify the current bacterial agents associated with early onset s ...201628036314
antimicrobial resistance of selected invasive bacteria in a tertiary care center: results of a prospective surveillance study.we aimed to report the distribution and resistance patterns of eight invasive clinically relevant bacteria surveyed in the clinical center of serbia (ccs) in belgrade.201628036312
etiological profile of early neonatal bacterial sepsis by multiplex qpcr.given the major impact in terms of morbidity and mortality that episodes of early neonatal sepsis (ens) have on both newborns and health systems, this study aimed to identify the etiological profile of early neonatal bacterial sepsis by a multiplex quantitative real-time polymerase chain reaction (qpcr).201628036311
probiotic properties of lactobacillus fermentum strains isolated from human oral samples and description of their antibacterial activity.gram positive bacteria produce peptides, defined bacteriocins which exhibit good antibacterial activity.201628034294
impact of polymicrobial biofilms in catheter-associated urinary tract infections.recent reports have demonstrated that most biofilms involved in catheter-associated urinary tract infections are polymicrobial communities, with pathogenic microorganisms (e.g. escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae) and uncommon microorganisms (e.g. delftia tsuruhatensis, achromobacter xylosoxidans) frequently co-inhabiting the same urinary catheter. however, little is known about the interactions that occur between different microorganisms and how they impact biofilm f ...201628033847
comparison of phenotypic tests and an immunochromatographic assay and development of a new algorithm for detection of oxa-48-like carbapenemases.oxa-48 is the most prevalent carbapenemase in enterobacteriaceae in europe and the middle east, but it is frequently missed because many isolates display low mics for carbapenems. furthermore, in contrast to metallo-β-lactamases or klebsiella pneumoniae carbapenemases (kpc), no specific inhibitor is available for the phenotypic detection of oxa-48. molecular detection of blaoxa-48 is the "gold standard" but is not available in many laboratories. a few phenotypic assays have been described but ha ...201728031433
copper is a host effector mobilized to urine during urinary tract infection to impair bacterial colonization.urinary tract infection (uti) is a major global infectious disease affecting millions of people annually. human urinary copper (cu) content is elevated during uti caused by uropathogenic escherichia coli (upec). upec upregulates the expression of cu efflux genes during clinical uti in patients as an adaptive response to host-derived cu. whether cu is mobilized to urine as a host response to uti and its role in protection against uti remain unresolved. to address these questions, we tested the hy ...201728031261
chromosomal integration of the klebsiella pneumoniae carbapenemase gene, blakpc, in klebsiella species is elusive but not rare.carbapenemase genes in enterobacteriaceae are mostly described as being plasmid associated. however, the genetic context of carbapenemase genes is not always confirmed in epidemiological surveys, and the frequency of their chromosomal integration therefore is unknown. a previously sequenced collection of blakpc-positive enterobacteriaceae from a single u.s. institution (2007 to 2012; n = 281 isolates from 182 patients) was analyzed to identify chromosomal insertions of tn4401, the transposon mos ...201728031204
emergence of ceftazidime-avibactam resistance due to plasmid-borne blakpc-3 mutations during treatment of carbapenem-resistant klebsiella pneumoniae infections.ceftazidime-avibactam is a novel β-lactam/β-lactamase inhibitor with activity against carbapenem-resistant enterobacteriaceae (cre) that produce klebsiella pneumoniae carbapenemase (kpc). we report the first cases of ceftazidime-avibactam resistance to develop during treatment of cre infections and identify resistance mechanisms. ceftazidime-avibactam-resistant k. pneumoniae emerged in three patients after ceftazidime-avibactam treatment for 10 to 19 days. whole-genome sequencing (wgs) of longit ...201728031201
low frequency of ceftazidime-avibactam resistance among enterobacteriaceae isolates carrying blakpc collected in u.s. hospitals from 2012 to 2015.klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae isolates have been increasingly reported worldwide, and therapeutic options to treat infections caused by these organisms are limited. we evaluated the activity of ceftazidime-avibactam and comparators against 456 enterobacteriaceae isolates carrying blakpc collected from 79 u.s. hospitals during 2012 to 2015. overall, ceftazidime-avibactam (mic50/90, 0.5/2 μg/ml; 99.3% susceptible) and tigecycline (mic50/90, 0.5/1 μg/ml; 98. ...201728031200
emergence of an extended-spectrum β-lactamase-producing serotype k1 klebsiella pneumoniae st23 strain from asian countries.extended-spectrum β-lactamase (esbl) production has been very rare in serotype k1 klebsiella pneumoniae st23 strains, which are well-known invasive community strains. among 92 esbl-producing strains identified in 218 isolates from nine asian countries, serotype k1 k. pneumoniae strains were screened. two esbl-producing k. pneumoniae isolates from singapore and indonesia were determined to be serotype k1 and st23. their plasmids, which contain ctx-m-15 genes, are transferable rendering the effect ...201728031071
surveillance of gram-negative bacteria: impact of variation in current european laboratory reporting practice on apparent multidrug resistance prevalence in paediatric bloodstream isolates.this study evaluates whether estimated multidrug resistance (mdr) levels are dependent on the design of the surveillance system when using routine microbiological data. we used antimicrobial resistance data from the antibiotic resistance and prescribing in european children (arpec) project. the mdr status of bloodstream isolates of escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa was defined using european centre for disease prevention and control (ecdc)-endorsed standardised a ...201628025699
thaulin-1: the first antimicrobial peptide isolated from the skin of a patagonian frog pleurodema thaul (anura: leptodactylidae: leiuperinae) with activity against escherichia coli.patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. in this sense, pleurodema thaul is the first amphibian specie to be studied from this large region of south america. analysis of cdna-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. the predicted mature peptides were synthesized and all of them showed weak or null antimi ...201728025119
cmc stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics.in the present study silver nanoparticles were synthesized by reduction of agno3 using aqueous cmc solution, which acts as both reducing and capping agent. the formation of agno3 nanoparticles was observed visually by color change and these nanoparticles were characterized through uv-vis spectroscopy, ftir, xrd, sem, eds and afm. the ftir peaks observed to be ranging from 3300 to 605cm(-1). the afm image clearly showed the surface morphology of well dispersed nanoparticles. sem image illustrates ...201728024537
pathogens and antimicrobial susceptibility profiles in critically ill patients with bloodstream infections: a descriptive study.surveillance of antimicrobial resistance is vital to guiding empirical treatment of infections. collating and reporting routine data on clinical isolate testing may offer more timely information about resistance patterns than traditional surveillance network methods.201728018869
leveraging the attributes of mucor hiemalis-derived silver nanoparticles for a synergistic broad-spectrum antimicrobial platform.driven by the need to engineer robust surface coatings for medical devices to prevent infection and sepsis, incorporation of nanoparticles has surfaced as a promising avenue to enhance non-fouling efficacy. microbial synthesis of such nanoscale metallic structures is of substantive interest as this can offer an eco-friendly, cost-effective, and sustainable route for further development. here we present a mucor hiemalis-derived fungal route for synthesis of silver nanoparticles, which display sig ...201628018316
preparation of membrane models of gram-negative bacteria and their interaction with antimicrobial peptides studied by cd and nmr.the antibiotic activity of antimicrobial peptides is generally derived via some type of disruption of the cell membrane(s). the most common models used to mimic the properties of bacterial membranes consist of mixtures of various zwitterionic and anionic phospholipids. this approach works reasonably well for gram-positive bacteria. however, since the membranes of gram-negative bacteria contain lipopolysaccharides, as well as zwitterionic and anionic phospholipids, a more complex model is require ...201728013508
comparing the outcomes of patients with carbapenemase-producing and non-carbapenemase-producing carbapenem-resistant enterobacteriaceae bacteremia.carbapenem-resistant enterobacteriaceae (cre) are associated with considerable mortality. as mechanisms of carbapenem resistance are heterogeneous, it is unclear if mortality differs based on resistance mechanisms. we sought to determine whether cre resistance mechanism determination is prognostically informative.201728013264
epidemiology of carbapenem-resistant klebsiella pneumoniae in a network of long-term acute care hospitals.the rapid emergence of carbapenem-resistant klebsiella pneumoniae (crkp) represents a major public health threat, including in the long-term acute care hospital (ltach) setting. our objective in this study was to describe the epidemiologic characteristics of crkp in a network of us ltachs.201728013258
might real-time pharmacokinetic/pharmacodynamic optimisation of high-dose continuous-infusion meropenem improve clinical cure in infections caused by kpc-producing klebsiella pneumoniae?the effect of real-time pharmacokinetic/pharmacodynamic (pk/pd) optimisation of high-dose continuous-infusion meropenem on the clinical outcome of patients receiving combination antimicrobial therapy for treatment of kpc-producing klebsiella pneumoniae (kpc-kp) infections was retrospectively assessed. data for all patients with kpc-kp-related infections who received antimicrobial combination therapy containing high-dose continuous-infusion meropenem optimised by means of therapeutic drug monitor ...201728012683
green production of microalgae-based silver chloride nanoparticles with antimicrobial activity against pathogenic bacteria.silver nanoparticles are powerful antimicrobial agents. here, the synthesis of silver chloride nanoparticles (agcl-nps) was consistently evidenced from a commercially valuable microalgae species, chlorella vulgaris. incubation of c. vulgaris conditioned medium with agno3 resulted in a medium color change to yellow/brown (with uv-vis absorbance at 415nm), indicative of silver nanoparticle formation. energy-dispersive x-ray spectroscopy (eds) of purified nanoparticles confirmed the presence of bot ...201728010768
[epidemiology and risk factors of patients with intra-abdominal postsurgical infection treated with tigecycline: a cohort study].to study a cohort of patients with intra-abdominal postsurgical infection treated with tigecycline to analyze its effectiveness and mortality related factors.201728010058
carbapenem-resistant enterobacteriaceae acquired before liver transplantation: impact on recipient outcomes.carbapenem-resistant enterobacteriaceae (cre) is an emergent microorganism of infections after liver transplant (lt). the aim of this study was to analyze the risk factors for cre acquisition and infection after lt.201728009779
[comparative analysis of antibacterial effect of phytoantibiotics and antibiotics].the objective of this study was to determine the antibacterial activity of individual medicines and containing sum of the phyto-extracts against the bacteria causing nosocomial infections in compare to antibiotics. in the investigation were involved four strains of gram-negative bacteria escherichia coli, klebsiella pneumoniae, staphylococcus aureus and acinetobacter spp. each object was investigated on antibiotic resistance using kirby-bauer diffusion method on 22 types of antibiotics. the obje ...201628009321
structural basis for the inhibition of recbcd by gam and its synergistic antibacterial effect with quinolones.our previous paper (wilkinson et al, 2016) used high-resolution cryo-electron microscopy to solve the structure of the escherichia coli recbcd complex, which acts in both the repair of double-stranded dna breaks and the degradation of bacteriophage dna. to counteract the latter activity, bacteriophage λ encodes a small protein inhibitor called gam that binds to recbcd and inactivates the complex. here, we show that gam inhibits recbcd by competing at the dna-binding site. the interaction surface ...201628009252
genome sequence of kp-rio/2015, a novel klebsiella pneumoniae (podoviridae) phage.klebsiella pneumoniae is a pathogen frequently associated with antibiotic-resistant nosocomial infections. here, we describe the genome of kp-rio/2015, a novel phage of k. pneumoniae belonging to the family podoviridae.201628007847
genetic characterisation of carbapenem-resistant gram-negative bacteria isolated from the university hospital mohamed boudiaf in ouargla, southern algeria.the aim of this study was to investigate the molecular epidemiology and the genetic support of carbapenem-resistant enterobacteriaceae and acinetobacter spp. isolates collected in the university hospital of ouargla, southern algeria.201728007519
a point-prevalence survey of healthcare-associated infection in fifty-two chinese hospitals.healthcare-associated infection (hcai) represents a major problem for patient safety worldwide.201728007308
clinical and bacterial risk factors for mortality in children with carbapenem- resistant enterobacteriaceae bloodstream infections in india.carbapenem-resistant enterobacteriaceae (cre) are an increasing cause of nosocomial infection in hospitalized children worldwide. few studies have investigated risk factors for mortality in children with cre bloodstream infection (bsi). data are particularly scarce in areas where ndm and oxa carbapenemases predominate. here, we investigate mortality rates, clinical and microbiologic risk factors for mortality in 50 pediatric patients with crbsi in india.201628005691
molecular typing and virulence analysis of multidrug resistant klebsiella pneumoniae clinical isolates recovered from egyptian hospitals.klebsiella pneumonia infection rates have increased dramatically. molecular typing and virulence analysis are powerful tools that can shed light on klebsiella pneumonia infections. whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (mdr) patterns, 50% (18/36) indicated carpabenem resistance. gene prevalence for the acrab efflux pump (82.14%) was more than that of the mdtk efflux pump (32.14%) in the mdr isolates. fimh-1 and mrkd genes were prevalent in wound and blood isola ...201628004732
antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative.explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach.201728004519
klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: in vitro study.this study evaluated the action of pfaffia paniculata k., juglans regia l., and rosmarius officinalis l. extracts against planktonic form and biofilm of klebsiella pneumoniae (atcc 4352). minimum inhibitory concentration (mic) and minimum microbicidal concentration (mmc) values were determined for each extract by microdilution broth method, according to clinical and laboratory standards institute. next, antimicrobial activity of the extracts on biofilm was analyzed. for this, standardized suspen ...201628004034
a rapid spin column-based method to enrich pathogen transcripts from eukaryotic host cells prior to sequencing.when analyzing pathogen transcriptomes during the infection of host cells, the signal-to-background (pathogen-to-host) ratio of nucleic acids (na) in infected samples is very small. despite the advancements in next-generation sequencing, the minute amount of pathogen na makes standard rna-seq library preps inadequate for effective gene-level analysis of the pathogen in cases with low bacterial loads. in order to provide a more complete picture of the pathogen transcriptome during an infection, w ...201628002481
secondary bacterial isolates from previously untreated buruli ulcer lesions and their antibiotic susceptibility patterns in southern nigeria.mycolactones, secreted by mycobacterium ulcerans, were previously believed to prevent super infection in buruli ulcer lesions. however, little is known about secondary bacterial infections in these lesions. this study evaluated contaminating bacterial flora and their antibiotic susceptibility patterns in cases of previously untreated buruli ulcer disease from three states in southern nigeria.201728001222
estimating the morbidity and mortality associated with infections due to multidrug-resistant bacteria (mdrb), france, 2012.a study based on 2007 data estimated that 386,000 infections due to multidrug-resistant bacteria (mdrb) occurred in europe that year and 25,000 patients died from these infections. our objective was to estimate the morbidity and mortality associated with these infections in france.201627999665
rapid eucast disc diffusion testing of mdr escherichia coli and klebsiella pneumoniae: inhibition zones for extended-spectrum cephalosporins can be reliably read after 6 h of incubation.the need for rapid antibiotic susceptibility testing increases with escalating levels of antimicrobial resistance in enterobacteriaceae. our objective was to evaluate the accuracy of reading eucast disc diffusion, rosco esbl and carbapenemase detection kits and the mast carbapenemase activity test (cat-id) disc, after 6 h of incubation.201727999046
evidence of transmission of an ndm-5-producing klebsiella pneumoniae in a healthcare facility in new zealand. 201727999016
[nosocomial septicemia in the early stage after stem cell transplantation in children with major β-thalassemia].to explore the clinical characteristics of nosocomial septicemia in the early stage after hematopoietic stem cell transplantation (hsct) in children with major β-thalassemia.201627998866
improvement of 1,3-propanediol production using an engineered cyanobacterium, synechococcus elongatus by optimization of the gene expression level of a synthetic metabolic pathway and production conditions.the introduction of a synthetic metabolic pathway consisting of multiple genes derived from various organisms enables cyanobacteria to directly produce valuable chemicals from carbon dioxide. we previously constructed a synthetic metabolic pathway composed of genes from escherichia coli, saccharomyces cerevisiae, and klebsiella pneumoniae. this pathway enabled 1,3-propanediol (1,3-pdo) production from cellular dhap via glycerol in the cyanobacterium, synechococcus elongatus pcc 7942. the product ...201727998670
plasmid-mediated or chromosomally mediated colistin resistance in klebsiella pneumoniae? 201727998571
intravenous colistin use for multidrug-resistant gram-negative infections in pediatric patients.the emergence of infections due to multidrug-resistant gram-negative bacilli (mdr-gnb) has led to the resurrection of colistin use. the data on colistin use and drug-related adverse effects in children are scarce.201627994915
klebsiella pneumoniae of suspected human origin from free-living common seals on the east coast of england. 201627993969
first report of klebsiella pneumoniae carbapenemase-producing k. pneumoniae sternum osteomyelitis relapsing 6 years after mediastinitis following cardiac surgery. 201727993526
corrigendum to "carbapenem susceptibility among escherichia coli, klebsiella pneumoniae, and enterobacter cloacae isolates obtained from patients in intensive care units in taiwan in 2005, 2007, and 2009" [diagn microbiol infect dis 2015;81(4):290-295]. 201727993423
emergence of sequence type 11 klebsiella pneumoniae coproducing ndm-1 and vim-1 metallo-β-lactamases in a greek hospital.sequence type 11 klebsiella pneumoniae, coproducing ndm-1 and vim-1 metallo-β-lactamases, were isolated in a greek hospital. blandm-1 was part of a tn125 derivative, located on an ~90-kb plasmid similar to the ndm-1-encoding plasmid pb-3002cz. blavim-1 was located in an in-e541-like integron, carried on a multireplicon (inca/c and incr) plasmid of ~180kb.201727993422
detection of a new class c β-lactamase (cmy-139) in klebsiella pneumoniae of food origin from india. 201727987443
detection of eskape bacterial pathogens at the point of care using isothermal dna-based assays in a portable degas-actuated microfluidic diagnostic assay platform.an estimated 1.5 billion microbial infections occur globally each year and result in ∼4.6 million deaths. a technology gap associated with commercially available diagnostic tests in remote and underdeveloped regions prevents timely pathogen identification for effective antibiotic chemotherapies for infected patients. the result is a trial-and-error approach that is limited in effectiveness, increases risk for patients while contributing to antimicrobial drug resistance, and reduces the lifetime ...201727986722
high rates of ctx-m-15-producing escherichia coli and klebsiella pneumoniae in wild boars and barbary macaques in algeria.the present study aimed to screen for the presence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in wild boars and barbary macaques in béjaïa and jijel, algeria.201727984780
assessment of extended-spectrum β-lactamases and integrons among enterobacteriaceae in device-associated infections: multicenter study in north of iran.device-associated nosocomial infections (da-nis), due to mdr enterobacteriaceae, are a major threat to patient safety in icus. we investigated on extended-spectrum β-lactamases (esbl) producing enterobacteriaceae and incidence of integrons in these bacteria isolated from ventilator-associated pneumonia (vap) and catheter-associated urinary tract infections (cautis) in 18 governmental hospitals in the north of iran.201627980729
epidemiology and biomolecular characterization of carbapenem-resistant klebsiella pneumoniae in an italian hospital.to describe the occurrence of crkp infections in a tertiary care hospital and to analyse the allelic profiles of the clinical strains involved and the most frequent carbapenemases.201627980379
genome sequences of multidrug-resistant, colistin-susceptible and -resistant klebsiella pneumoniae clinical isolates from pakistan.the emergence and spread of colistin resistance among multidrug-resistant (mdr) klebsiella pneumoniae represent a critical threat to global health. here, we report the complete genome sequences of 10 mdr, colistin-susceptible and -resistant k. pneumoniae clinical isolates obtained in pakistan between 2010 and 2013.201627979956
complete genome sequence of community-acquired klebsiella pneumoniae kp36, a strain isolated from a patient with an upper urinary tract infection.here, we announce the complete genome sequence of klebsiella pneumoniae kp36, a strain isolated from a patient with a severe community-acquired urinary tract infection. this genome provides insights into the pathogenesis of a pandemic k. pneumoniae strain from a community-acquired urinary tract infection.201627979951
procalcitonin levels in bloodstream infections caused by different sources and species of bacteria.the aim of this study was to evaluate procalcitonin (pct) diagnostic accuracy in discriminating gram-negative (gn) from gram-positive (gp) bloodstream infections and determining the relationship between pct levels, infection sites, and pathogen types.201727979420
novel antibacterial compounds and their drug targets - successes and challenges.infectious diseases are one of the most important and urgent health problems in the world. according to the world health organization (who) statistics, infectious and parasitic diseases are a cause of about 16% of all deaths worldwide and over 40% of deaths in africa. a considerable progress that has been made during last hundred years in the fight against infectious diseases, in particular bacterial infections, can be attributed mainly to three factors: (1) the general improvement of living con ...201627978802
high-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader.routine antimicrobial susceptibility testing (ast) can prevent deaths due to bacteria and reduce the spread of multi-drug-resistance, but cannot be regularly performed in resource-limited-settings due to technological challenges, high-costs, and lack of trained professionals. we demonstrate an automated and cost-effective cellphone-based 96-well microtiter-plate (mtp) reader, capable of performing ast without the need for trained diagnosticians. our system includes a 3d-printed smartphone attach ...201627976700
a novel microfluidic assay for rapid phenotypic antibiotic susceptibility testing of bacteria detected in clinical blood cultures.appropriate antibiotic therapy is critical in the management of severe sepsis and septic shock to reduce mortality, morbidity and health costs. new methods for rapid antibiotic susceptibility testing are needed because of increasing resistance rates to standard treatment.201627974860
antibiofilm and anti-β-lactamase activities of burdock root extract and chlorogenic acid against klebsiella pneumoniae.small phytochemicals have been successfully adopted as antibacterial chemotherapies and are being increasingly viewed as potential antibiofilm agents. some of these molecules are known to repress biofilm and toxin production by certain bacterial and yeast pathogens, but information is lacking with regard to the genes allied with biofilm formation. the present study was performed to investigate the inhibitory effect of burdock root extract (bre) and of chlorogenic acid (cga; a component of bre) o ...201727974734
tigecycline therapy in an infant for ventriculoperitoneal shunt meningitis.shunt infections are seen in 3% to 20% of patients who have cerebrospinal fluid (csf) shunts. although the staphylococcal species are the most common cause of shunt-related infections, gram-negative bacteria are increasingly reported with higher mortality rates. tigecycline, a glycylcycline, is not approved for children. but in the era of nosocomial infections due to multidrug-resistant pathogens, it can be the life-saving option. we report an infant with ventriculoperitoneal shunt-related menin ...201727974589
multiple variants of klebsiella pneumoniae producing carbapenemase in one patient. 201627974041
in situ synthesis of high swell ratio polyacrylic acid/silver nanocomposite hydrogels and their antimicrobial properties.silver nanocomposites embedded within a polymer matrix have attracted attention in recent years. ionic polymer hydrogels comprise networks of chemically or physically cross-linked polymers that swell considerably in an appropriate solvent. in this study, we used a solution of the carboxylic monomer acrylic acid and silver nitrate to prepare nanocomposite hydrogels through ultraviolet (uv)-light irradiation. silver-impregnated biomaterial composed of acrylic acid contains only a monomer and no cr ...201627968959
clinical and genomic analysis of liver abscess-causing klebsiella pneumoniae identifies new liver abscess-associated virulence genes.hypervirulent variants of klebsiella pneumoniae (hvkp) that cause invasive community-acquired pyogenic liver abscess (pla) have emerged globally. little is known about the virulence determinants associated with hvkp, except for the virulence genes rmpa/a2 and siderophores (irobcd/iucabcd) carried by the pk2044-like large virulence plasmid. here, we collected most recent clinical isolates of hvkp from pla samples in china, and performed clinical, molecular, and genomic sequencing analyses. we fou ...201627965935
high prevalence of gut microbiota colonization with broad-spectrum cephalosporin resistant enterobacteriaceae in a tunisian intensive care unit.healthcare-associated infections due to cefotaxime-resistant (ctx-r) enterobacteriaceae have become a major public health threat, especially in intensive care units (icus). often acquired nosocomially, ctx-r enterobacteriaceae can be introduced initially by patients at admission. this study aimed to determine the prevalence and genetic characteristics of ctx-r enterobacteriaceae-intestinal carriage in icu patients, to evaluate the rate of acquisition of these organisms during hospitalization, an ...201627965626
comprehensive genome analysis of carbapenemase-producing enterobacter spp.: new insights into phylogeny, population structure, and resistance mechanisms.knowledge regarding the genomic structure of enterobacter spp., the second most prevalent carbapenemase-producing enterobacteriaceae, remains limited. here we sequenced 97 clinical enterobacter species isolates that were both carbapenem susceptible and resistant from various geographic regions to decipher the molecular origins of carbapenem resistance and to understand the changing phylogeny of these emerging and drug-resistant pathogens. of the carbapenem-resistant isolates, 30 possessed blakpc ...201627965456
molecular and in silico analysis of a new plasmid-mediated ampc β-lactamase (cmh-2) in clinical isolates of klebsiella pneumoniae.two klebsiella strains isolated from urine samples were positive for blaampc by pcr and showed sequence similarity with cmh-1 (98.6%) after sequencing. it also shares 82% similarity with act-1, 85% with mir-1 and 81% with the chromosomal ampc gene of enterobacter cloacae. this gene was associated with the plasmid of inck type. it has an open reading frame of 381 amino acid with four amino acid substitutions at position d144a, c189r, q192e, and a195t as compared to cmh-1. when expressed in e.coli ...201727964935
antimicrobial resistance pattern: a report of microbiological cultures at a tertiary hospital in tanzania.antimicrobial resistance has been declared by the world health organization as a threat to the public health. the aim of this study was to analyze antimicrobial resistance patterns of the common pathogens occurring at the bugando medical centre (bmc), mwanza, tanzania to provide data for antimicrobial stewardship programmes.201627964724
bionano interaction study on antimicrobial star-shaped peptide polymer nanoparticles.'structurally nanoengineered antimicrobial peptide polymers' (snapps), in the form of star-shaped peptide polymer nanoparticles, have been recently demonstrated as a new class of antimicrobial agents with superior in vitro and in vivo efficacy against gram-negative pathogens, including multidrug-resistant species. herein, we present a detailed bionano interaction study on snapps by assessing their antimicrobial activities against several gram-negative bacteria in complex biological matrices. sim ...201627960388
community-acquired pneumonia in the asia-pacific region.community-acquired pneumonia (cap) is an important cause of mortality and morbidity worldwide. aging population, dense urbanization, and poor access to health care make the asia-pacific region vulnerable to cap. the high incidence of cap poses a significant health and economic burden in this region. common etiologic agents in other global regions including streptococcus pneumoniae, mycoplasma pneumoniae, haemophilus influenzae, chlamydophila pneumoniae, staphylococcus aureus, and respiratory vir ...201627960208
multiple-locus variable number tandem repeat analysis of klebsiella pneumoniae: comparison with pulsed-field gel electrophoresis.to assess whether multiple-locus variable-number tandem repeat analysis (mlva) could replace pulsed-field gel electrophoresis (pfge) for genotyping of klebsiella pneumoniae, this study was conducted to compare the typeability, discriminatory power, and concordance of these methods.201627960064
clinical features and outcomes of ipf patients hospitalized for pulmonary infection: a japanese cohort study.many patients with idiopathic pulmonary fibrosis (ipf) undergo hospitalizations due to pulmonary infections. we retrospectively investigated the characteristics of hospitalizations due to pulmonary infection in patients with ipf to elucidate causative pathogens and mortality. we reviewed patients with ipf who were admitted between january 2008 and december 2014 for pulmonary infections including pneumonia and bronchitis. the causative pathogen, the relationship between the site of pneumonia and ...201627959904
survival of antibacterial resistance microbes in hospital-generated recycled wastewater.hospital wastewater has the potential to be a threat to the hospital environment as it can contain pathogenic bacteria that may facilitate the resistant nature of organisms within effluent or water treatment plants. the recycling of hospital wastewater should have good quality. this study was carried out to highlight the incidence of antibiotic resistant bacteria in hospital-generated recycled water. this study was conducted in a tertiary care teaching hospital during june 2013-june 2014. one hu ...201627959872
influence of fimbriae on bacterial adhesion and viscoelasticity and correlations of the two properties with biofilm formation.the surface polymers of bacteria determine the ability of bacteria to adhere to a substrate for colonization, which is an essential step for a variety of microbial processes, such as biofilm formation and biofouling. capsular polysaccharides and fimbriae are two major components on a bacterial surface, which are critical for mediating cell-surface interactions. adhesion and viscoelasticity of bacteria are two major physical properties related to bacteria-surface interactions. in this study, we e ...201727959542
inventory of extended-spectrum-β-lactamase-producing enterobacteriaceae in france as assessed by a multicenter study.the objective of this study was to perform an inventory of the extended-spectrum-β-lactamase (esbl)-producing enterobacteriaceae isolates responsible for infections in french hospitals and to assess the mechanisms associated with esbl diffusion. a total of 200 nonredundant esbl-producing enterobacteriaceae strains isolated from clinical samples were collected during a multicenter study performed in 18 representative french hospitals. antibiotic resistance genes were identified by pcr and sequenc ...201727956424
distribution of ctx-m group i and group iii β-lactamases produced by escherichia coli and klebsiella pneumoniae in lahore, pakistan.extended-spectrum-lactamases (esbls) of the ctx-m type is worrisome issue in many countries of the world from past decade. but little is known about ctx-m beta-lactamase producing bacteria in pakistan. therefore, this study was carried out to investigate the distribution of ctx-m beta-lactamase producing e. coli and klebsiella pneumoniae using phenotypic and molecular techniques. a total of 638 e. coli and 338 klebsiella pneumoniae were isolated from patients attending two hospitals and one diag ...201727956216
carbapenem-resistant klebsiella pneumoniae infection in three new york city hospitals trended downwards from 2006 to 2014.carbapenem-resistant klebsiella pneumoniae (crkp) infection is a rising public health threat since its first outbreaks in new york city (nyc) in the early 2000s. we investigated annual trends of crkp infection in hospital-acquired infections (hais) and community-onset infections (cois) treated in 3 nyc hospitals from 2006 to 2014.201627942542
surveillance of antibiotic-resistant bacteria in king khalid hospital, hafr al-batin, saudi arabia, during 2013.research to understand and control the emergence and spread of antimicrobial resistance has become a public health priority.201627942366
ndm-1 and rmtc-producing klebsiella pneumoniae isolates in turkey.the resistance of aminoglycosides in strains that produce beta-lactamase can be developed through the multidrug resistant encoding genes carried by common plasmids. recently, the association between 16s rrna methyltransferase resistance and beta-lactamase enzymes carried by the same plasmids has drawn increased attention from researchers, particularly the association in aminoglycoside-resistant strains with a minimum inhibitory concentration (mic) of ≥ 256 µg/ml.201627942358
isolation of extended spectrum β-lactamase producing gram negative bacilli from wound swab with their antimicrobial susceptibility pattern.extended spectrum β-lactamases (esbls) producing organisms have become the major clinical concern worldwide. the present study was undertaken to see the frequency of esbls producing gram-negative bacilli with their antibiogram in post surgical wound swab collected over a period of 12 months from july 2011 to june 2012 at dhaka medical college hospital. among 200 samples 121(60.5%) gram negative bacilli and 52(26%) gram-positive bacteria were isolated. escherichia coli (36.42%) was the most predo ...201627941735
colistin resistance in carbapenem-resistant klebsiella pneumoniae: laboratory detection and impact on mortality.polymyxins including colistin are an important "last-line" treatment for infections caused by carbapenem-resistant klebsiella pneumoniae (crkp). increasing use of colistin has led to resistance to this cationic antimicrobial peptide.201727940944
incidence, risk factors and associated mortality of central line-associated bloodstream infections at an intensive care unit in northern india.to evaluate the incidence, risk factors and associated mortality of central line-associated bloodstream infection (clabsi) in an adult intensive care unit (icu) in india.201627940521
clbm is a versatile, cation-promiscuous mate transporter found in the colibactin biosynthetic gene cluster.multidrug transporters play key roles in cellular drug resistance to toxic molecules, yet these transporters are also involved in natural product transport as part of biosynthetic clusters in bacteria and fungi. the genotoxic molecule colibactin is produced by strains of virulent and pathobiont escherichia coli and klebsiella pneumoniae. in the biosynthetic cluster is a multidrug and toxic compound extrusion protein (mate) proposed to transport the prodrug molecule precolibactin across the cytop ...201727939886
first report of ndm-1-producing klebsiella pneumoniae imported from africa to italy: evidence of the need for continuous surveillance.ndm-producing enterobacteriaceae are considered emergent on the african continent and have been increasingly reported in recent years. in contrast, strains producing ndm-type enzymes have been rarely reported in italy, usually associated with sporadic cases or small outbreaks. here we report two cases of infection caused by ndm-1-producing klebsiella pneumoniae (ndm-kp) in two unrelated patients returned from travel to egypt.201727939808
integration of biomass formulations of genome-scale metabolic models with experimental data reveals universally essential cofactors in prokaryotes.the composition of a cell in terms of macromolecular building blocks and other organic molecules underlies the metabolic needs and capabilities of a species. although some core biomass components such as nucleic acids and proteins are evident for most species, the essentiality of the pool of other organic molecules, especially cofactors and prosthetic groups, is yet unclear. here we integrate biomass compositions from 71 manually curated genome-scale models, 33 large-scale gene essentiality data ...201727939572
anthropogenic antibiotic resistance genes mobilization to the polar regions.anthropogenic influences in the southern polar region have been rare, but lately microorganisms associated with humans have reached antarctica, possibly from military bases, fishing boats, scientific expeditions, and/or ship-borne tourism. studies of seawater in areas of human intervention and proximal to fresh penguin feces revealed the presence of escherichia coli strains least resistant to antibiotics in penguins, whereas e. coli from seawater elsewhere showed resistance to one or more of the ...201627938628
assessment of antibiotic resistance in klebsiella pneumoniae exposed to sequential in vitro antibiotic treatments.bacteria treated with different classes of antibiotics exhibit changes in susceptibility to successive antibiotic treatments. this study was designed to evaluate the influence of sequential antibiotic treatments on the development of antibiotic resistance in klebsiella pneumoniae associated with β-lactamase and efflux pump activities.201627938381
detection of acra, acrb, aac(6')-ib-cr, and qepa genes among clinical isolates of escherichia coli and klebsiella pneumoniae.the distribution of drug resistance among clinical isolates of escherichia coli and klebsiella pneumoniae has limited the therapeutic options. the aim of this study was to report the prevalence of quinolone resistance genes among e. coli and k. pneumoniae clinical strains isolated from three educational hospitals of tehran, iran.201727936860
quantitative optical coherence microscopy for the in situ investigation of the biofilm.this paper explores the potential of optical coherence microscopy (ocm) for the <italic<in situ</italic< monitoring of biofilm growth. the quantitative imaging of the early developmental biology of a representative biofilm, <italic<klebsiella pneumonia</italic< (kp-1), was performed using a swept source-based fourier domain ocm system. the growth dynamics of the kp-1 biofilms and their transient response under perturbation was investigated using the enface visualization of microcolonies and thei ...201627936266
high prevalence of faecal carriage of esbl-producing enterobacteriaceae among children in dar es salaam, tanzania.faecal carriage of esbl-producing bacteria is a potential risk for transmission and infection. little is known about faecal carriage of antibiotic resistance in tanzania. this study aimed to investigate the prevalence of faecal carriage of esbl-producing enterobacteriaceae and to identify risk factors for carriage among young children in tanzania.201627936054
molecular identification of extended-spectrum β-lactamase and integron genes in klebsiella pneumonia.infections caused by gram negative bacteria, producing extended-spectrum β-lactamase, including klebsiella pneumoniae are increasing all over the world with high morbidity and mortality. the aim of the present study was determined antimicrobial profile susceptibility and the prevalence of antibiotic resistance genes by multiplex pcr.201627935927
siderophores: more than stealing iron.siderophores are small molecular iron chelators that are produced by microbes and whose most notable function is to sequester iron from the host and provide this essential metal nutrient to microbes. recent studies have proposed additional, noncanonical roles for siderophores, including the acquisition of noniron metals and modulation of host functions. recently, holden et al. (v. i. holden, p. breen, s. houle, c. m. dozois, and m. a. bachman, mbio 7:e01397-16, 2016, http://dx.doi.org/10.1128/mb ...201627935843
from breast cancer to antimicrobial: combating extremely resistant gram-negative "superbugs" using novel combinations of polymyxin b with selective estrogen receptor modulators.novel therapeutic approaches are urgently needed to combat nosocomial infections caused by extremely drug-resistant (xdr) "superbugs." this study aimed to investigate the synergistic antibacterial activity of polymyxin b in combination with selective estrogen receptor modulators (serms) against problematic gram-negative pathogens. in vitro synergistic antibacterial activity of polymyxin b and the serms tamoxifen, raloxifene, and toremifene was assessed using the microdilution checkerboard and st ...201627935770
intense pre-admission carriage and further acquisition of esbl-producing enterobacteriaceae among patients and their caregivers in a tertiary hospital in rwanda.to assess the presence and risk factors of intestinal carriage of extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-pe) among patients admitted to the university teaching hospital of butare and among their attending caregivers, and to analyse the acquisition of esbl-pe carriage during hospital stay and associated factors.201727935649
erratum: endemic indian clones of klebsiella pneumoniae harbouring new delhi metallo beta lactamase 1 on a hybrid plasmid replicon type: a case of changing new delhi metallo beta lactamase plasmid landscapes in india?[this corrects the article doi: 10.4103/0255-0857.188314.].201727934850
does the presence of klebsiella pneumoniae carbapenemase and new delhi metallo-β-lactamase-1 genes in pathogens lead to fatal outcome?infections due to multidrug-resistant (mdr) pathogens are a medical challenge. there is considerable apprehension among clinicians regarding pathogens reported as carrying new delhi metallo-β-lactamase-1 (ndm) and klebsiella pneumoniae carbapenemase (kpc) genes from their patients. in the face of extremely high rates of antimicrobial resistance, it is essential to gauge the clinical significance of isolation of pathogens carrying these genes from clinical samples. this study compares the outcome ...201727934829
emergence of serotype k1 klebsiella pneumoniae st23 strains co-producing the plasmid-mediated ampc beta-lactamase dha-1 and an extended-spectrum beta-lactamase in korea.serotype k1 klebsiella pneumoniae has emerged as an important community pathogen causing various infections, including liver abscesses. although serotype k1 k. pneumoniae community isolates have been reported as susceptible to most classes of antimicrobial agents, a few cases of infection caused by extended-spectrum beta-lactamase (esbl)-producing serotype k1 k. pneumoniae have recently been reported in asian countries. we identified three esbl-producing strains of serotype k1 k. pneumoniae and ...201627933141
draft genome sequences of three hypervirulent carbapenem-resistant klebsiella pneumoniae isolates from bacteremia.hypervirulent klebsiella pneumoniae strains have been increasingly reported worldwide, and there is emergence of carbapenem resistance among them. here, we report the genome sequences of three carbapenem-resistant hypervirulent k. pneumoniae isolates isolated from bacteremic patients at a tertiary-care center in south india.201627932638
searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds.the aims of this study were to design, synthesize and to evaluate 2-hydroxy-3-phenylsulfanylmethyl-[1,4]-naphthoquinones against gram-negative and gram-positive bacterial strains, including methicillin-resistant staphylococcus aureus (mrsa) and its biofilm, to probe for potential lead structures.201727930849
expanding the klebsiella pneumoniae volatile metabolome using advanced analytical instrumentation for the detection of novel metabolites.the purpose of this study was to identify the volatile molecules produced by the pathogenic gram-negative bacterium klebsiella pneumoniae (atcc 13883) during in vitro growth using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (gc×gc-tofms).201727930839
Displaying items 901 - 1000 of 38507